Protein Expr Purif 2021 May 12:105906. Epub 2021 May 12.
Laboratory of Cellular and Molecular Interactions (CMIM), Vrije Universiteit Brussels, Brussels, Belgium; Laboratory for Biomedical Research, Ghent University Global Campus, Yeonsu-Gu, Incheon, South Korea; Department of Biochemistry and Microbiology, Universiteit Gent, Ledeganckstraat 35, 9000, Gent, Belgium. Electronic address:
Nanobodies are single-domain antibody constructs derived from the variable regions of heavy chain only (VH) camelid IgGs. Their small size and single gene format make them amenable to various molecular biology applications that require a protein affinity-based approach. These features, in addition to their high solubility, allows their periplasmic expression, extraction and purification in E. Read More