1,432,637 results match your criteria antibody


A high sensitive platinum-modified colloidal gold immunoassay for tenuazonic acid detection based on monoclonal IgG.

Food Chem 2021 May 6;360:130021. Epub 2021 May 6.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address:

Due to the threat of tenuazonic acid (TA) to public health, it is urgent to establish rapidly effective and sensitive assay methods for TA. In this study, a TA-specific IgG monoclonal antibody (McAb) with high affinity (Kaff was 1.72 × 10 L/mol) was screened, and the developed icELISA for TA detection has IC of 2. Read More

View Article and Full-Text PDF

An intervention to increase hepatitis C virus diagnosis and treatment uptake among people in custody in Iran.

Int J Drug Policy 2021 May 12;95:103269. Epub 2021 May 12.

Digestive Oncology Research Center, Digestive Diseases Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Background: Iran is among countries with high opioid agonist therapy (OAT) coverage in prisons, which provides an infrastructure to increase feasibility of HCV programs. We aimed to evaluate the impact of an intervention to improve HCV screening, diagnosis, and treatment, including alongside the provision of OAT, in an Iranian prison.

Methods: During July-December 2018, in the Gorgan prison, all incarcerated adults (>18 years) received HCV antibody rapid testing and, if positive, provided a venepuncture sample for HCV RNA testing. Read More

View Article and Full-Text PDF

STAT6: A review of a signaling pathway implicated in various diseases with a special emphasis in its usefulness in pathology.

Pathol Res Pract 2021 May 11;223:153477. Epub 2021 May 11.

Pathology Department, University Hospital of Saint-Etienne, France.

Signal Transducer and Activator of Transcription 6 (STAT6), belonging to a family of seven similar members is primarily stimulated by interleukin(IL)-4 and IL-13, and acts as a T helper type 2 (Th2)-inducing factor. Thus, it is implicated in the pathophysiology of various allergic conditions, such as asthma, atopic dermatitis, eosinophilic esophagitis and food allergies, but also in tumor microenvironment regulation. Furthermore, certain forms of lymphomas, notably the Hodgkin lymphoma group, the primary mediastinal and primary central nervous system lymphoma, as well as some follicular and T cell lymphomas are associated with dysregulation of the STAT6 pathway. Read More

View Article and Full-Text PDF

In-capillary (electrophoretic) digestion-reduction-separation: A smart tool for middle-up analysis of mAb.

J Chromatogr A 2021 May 3;1648:462213. Epub 2021 May 3.

Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM-ENSCM, Université de Montpellier, Montpellier, France. Electronic address:

Comprehensive characterization of physicochemical properties of monoclonal antibodies (mAbs) is a critical process to ensure their quality, efficacy, and safety. For this purpose, mAb analysis at different levels (bottom-up, middle-up) is a common approach that includes rather complex multistep sample preparation (reduction, digestion). To ensure high analysis performance, the development of fully integrated methodologies is highly valuable. Read More

View Article and Full-Text PDF

Pre-transplant red blood cell and platelet transfusion burden in de novo myelodysplastic syndrome undergoing allogeneic transplantation: Pre-transplant red blood cell and platelet transfusion burden in de novo MDS after allogeneic transplantation.

Transplant Cell Ther 2021 May 12. Epub 2021 May 12.

Japanese Data Center for Hematopoietic Cell Transplantation, Nagoya, Japan; Department of Healthcare Administration, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Background: Most patients of myelodysplastic syndrome (MDS) require red blood cell (RBC) and/or platelet transfusion during their disease courses, which could cause an increased risk of iron overload and alloimmunization. However, it remains less clear whether pre-transplant RBC or platelet transfusion burden affects transplant outcomes in patients with MDS.

Objective: The objective was to examine the significance of pre-transplant RBC and platelet transfusion burden on transplant outcomes after allogeneic HCT for adults with de novo MDS. Read More

View Article and Full-Text PDF

Reverse phase protein array reveals correlation of retinoic acid metabolism with cardiomyopathy in Friedreich's ataxia.

Mol Cell Proteomics 2021 May 12:100094. Epub 2021 May 12.

University of Alabama at Birmingham, Department of Biochemistry and Molecular Genetics, 1825 University Blvd., Birmingham, Alabama 35294, USA. Electronic address:

Identifying biomarkers is important for assessment of disease progression, prediction of symptom development, and determination of treatment effectiveness. While unbiased analyses of differential gene expression using next generation sequencing methods are now routinely conducted, proteomics studies are more challenging due to traditional methods predominantly being low-throughput and offering a limited dynamic range for simultaneous detection of hundreds of proteins that drastically differ in their intracellular abundance. We utilized a sensitive and high-throughput proteomic technique, reverse phase protein array (RPPA), to attain protein expression profiles of primary fibroblasts obtained from Friedreich's ataxia (FRDA) patients and unaffected controls. Read More

View Article and Full-Text PDF

Improving the yield of recalcitrant Nanobodies® by simple modifications to the standard protocol.

Protein Expr Purif 2021 May 12:105906. Epub 2021 May 12.

Laboratory of Cellular and Molecular Interactions (CMIM), Vrije Universiteit Brussels, Brussels, Belgium; Laboratory for Biomedical Research, Ghent University Global Campus, Yeonsu-Gu, Incheon, South Korea; Department of Biochemistry and Microbiology, Universiteit Gent, Ledeganckstraat 35, 9000, Gent, Belgium. Electronic address:

Nanobodies are single-domain antibody constructs derived from the variable regions of heavy chain only (VH) camelid IgGs. Their small size and single gene format make them amenable to various molecular biology applications that require a protein affinity-based approach. These features, in addition to their high solubility, allows their periplasmic expression, extraction and purification in E. Read More

View Article and Full-Text PDF

A clinical prediction model to distinguish maturity-onset diabetes of the young from type 1 and type 2 diabetes in the Chinese population.

Endocr Pract 2021 May 12. Epub 2021 May 12.

Department of Endocrinology, Key Laboratory of Endocrinology of the Ministry of Health, Peking Union Medical Collage Hospital, Chinese Academy of Medical Science; Shuaifuyuan No.1, Dongcheng District, Beijing, 100730, China. Electronic address:

Objective: Genetic detection for the diagnosis of maturity-onset diabetes of the young (MODY) in China has low sensitivity and specificity. Better gene detection is urgently needed to distinguish testing subjects. We proposed to use numerous and weighted clinical traits as key indicators for reasonable genetic testing to predict the probability of MODY in the Chinese population. Read More

View Article and Full-Text PDF

Elezanumab, a human anti-RGMa monoclonal antibody, promotes neuroprotection, neuroplasticity, and neurorecovery following a thoracic hemi-compression spinal cord injury in non-human primates.

Neurobiol Dis 2021 May 12:105385. Epub 2021 May 12.

Department of Neurosurgery and The Miami Project to Cure Paralysis, The Miller School of Medicine, University of Miami, Miami, FL, United States.

Spinal cord injury (SCI) is a devastating condition characterized by loss of function, secondary to damaged spinal neurons, disrupted axonal connections, and myelin loss. Spontaneous recovery is limited, and there are no approved pharmaceutical treatments to reduce ongoing damage or promote repair. Repulsive guidance molecule A (RGMa) is upregulated following injury to the central nervous system (CNS), where it is believed to induce neuronal apoptosis and inhibit axonal growth and remyelination. Read More

View Article and Full-Text PDF

Immunoinformatics and Pepscan strategies on the path of a peptide-based serological diagnosis of COVID19.

J Immunol Methods 2021 May 12:113071. Epub 2021 May 12.

Unidad de Química Medicinal, Facultad de Farmacia, Universidad Central de Venezuela, Caracas 1051, Venezuela. Electronic address:

Several diagnostic tools have been developed for clinical and epidemiological assays. RT-PCR and antigen detection tests are more useful for diagnosis of acute disease, while antibody tests allow the estimation of exposure in the population. Currently, there is an urgent need for the development of diagnostic tests for COVID-19 that can be used for large-scale epidemiological sampling. Read More

View Article and Full-Text PDF

Follicular Cells in Pituitary Neuroendocrine Tumors.

Hum Pathol 2021 May 12. Epub 2021 May 12.

Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH USA, Case Western Reserve University School of Medicine, Cleveland, OH, 44106, USA. Electronic address:

Follicular cells (FCs) are thought to be agranular, non-hormone producing stellate cells distributed throughout the adenohypophysis, occasionally arranged around colloid-filled follicles, and thought to be more prominent in the vicinity of necrosis and apoptotic cells. A distinct but similar cell type, the folliculostellate cell (FSC), is a sustentacular cell that is negative for keratins and stains for S100, GFAP and SOX10. While several studies have examined FSCs in pituitary neuroendocrine tumors (PitNETs), the distribution and derivation of FCs in these lesions is unclear. Read More

View Article and Full-Text PDF

Consideration triggered by the choice of heterophilic antibody interference detection tests in measuring ACTH for a teenager boy with a rare adrenal disease.

Clin Chim Acta 2021 May 12. Epub 2021 May 12.

Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing 100730, P.R. China. Electronic address:

Background: Plasma ACTH is an important marker for Cushing syndrome; inaccurate measurement of ACTH concentrations due to the serum heterophile antibody may mislead diagnosis by physicians. We describe the case of a teenage boy with a rare adrenal disease who presented falsely increased ACTH concentrations (20.3 pg/ml; Siemens Immulite). Read More

View Article and Full-Text PDF

Enfortumab vedotin after PD-1 or PD-L1 inhibitors in cisplatin-ineligible patients with advanced urothelial carcinoma (EV‑201): a multicentre, single-arm, phase 2 trial.

Lancet Oncol 2021 May 12. Epub 2021 May 12.

Perlmutter Cancer Center at NYU Langone Health, New York, NY, USA.

Background: Locally advanced or metastatic urothelial carcinoma is generally incurable and has scarce treatment options, especially for cisplatin-ineligible patients previously treated with PD-1 or PD-L1 therapy. Enfortumab vedotin is an antibody-drug conjugate directed at Nectin-4, a protein highly expressed in urothelial carcinoma. We aimed to evaluate the efficacy and safety of enfortumab vedotin in the post-immunotherapy setting in cisplatin-ineligible patients. Read More

View Article and Full-Text PDF

Epitope profiling reveals binding signatures of SARS-CoV-2 immune response in natural infection and cross-reactivity with endemic human CoVs.

Cell Rep 2021 May 6:109164. Epub 2021 May 6.

Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. Electronic address:

A major goal of current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine efforts is to elicit antibody responses that confer protection. Mapping the epitope targets of the SARS-CoV-2 antibody response is critical for vaccine design, diagnostics, and development of therapeutics. Here, we develop a pan-coronavirus phage display library to map antibody binding sites at high resolution within the complete viral proteomes of all known human-infecting coronaviruses in patients with mild or moderate/severe coronavirus disease 2019 (COVID-19). Read More

View Article and Full-Text PDF

Dynamics of B cell repertoires and emergence of cross-reactive responses in patients with different severities of COVID-19.

Cell Rep 2021 May 9:109173. Epub 2021 May 9.

HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China; Li Ka Shing Institute of Health Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China; The Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong SAR, China. Electronic address:

Individuals with the 2019 coronavirus disease (COVID-19) show varying severity of the disease, ranging from asymptomatic to requiring intensive care. Although monoclonal antibodies specific to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been identified, we still lack an understanding of the overall landscape of B cell receptor (BCR) repertoires in individuals with COVID-19. We use high-throughput sequencing of bulk and plasma B cells collected at multiple time points during infection to characterize signatures of the B cell response to SARS-CoV-2 in 19 individuals. Read More

View Article and Full-Text PDF

A novel nano therapeutic using convalescent plasma derived exosomal (CP) for COVID-19: A combined hyperactive immune modulation and diagnostics.

Chem Biol Interact 2021 May 12:109497. Epub 2021 May 12.

Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Ultimo, NSW, 2007, Australia; Priority Research Centre for Healthy Lungs, Hunter Medical Research Institute (HMRI), University of Newcastle, New Lambton Heights, Newcastle, NSW 2305, Australia.

Extracellular vesicles like exosomes are important therapeutic tactics for treating COVID -19. By utilizing convalescent plasma derived exosomes (CP) from COVID-19 recovered persistence could accelerate the treatment strategies in the current state of affairs. Adequate literature has shown that administering the exosome to the in vivo system could be beneficial and could target the pathogens in an effective and precise manner. Read More

View Article and Full-Text PDF

Transmission, infectivity, and neutralization of a spike L452R SARS-CoV-2 variant.

Cell 2021 Apr 20. Epub 2021 Apr 20.

Los Angeles County Public Health Laboratories, Downey, CA 90242, USA.

We identified an emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant by viral whole-genome sequencing of 2,172 nasal/nasopharyngeal swab samples from 44 counties in California, a state in the western United States. Named B.1. Read More

View Article and Full-Text PDF

SUCLA2-coupled regulation of GLS succinylation and activity counteracts oxidative stress in tumor cells.

Mol Cell 2021 May 8. Epub 2021 May 8.

Department of Hepatobiliary and Pancreatic Surgery and Zhejiang Provincial Key Laboratory of Pancreatic Disease of The First Affiliated Hospital, Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310029, China; Zhejiang University Cancer Center, Hangzhou, Zhejiang 310029, China. Electronic address:

Glutaminase regulates glutaminolysis to promote cancer cell proliferation. However, the mechanism underlying glutaminase activity regulation is largely unknown. Here, we demonstrate that kidney-type glutaminase (GLS) is highly expressed in human pancreatic ductal adenocarcinoma (PDAC) specimens with correspondingly upregulated glutamine dependence for PDAC cell proliferation. Read More

View Article and Full-Text PDF

No strong HLA association with MOG antibody disease in the UK population.

Ann Clin Transl Neurol 2021 May 15. Epub 2021 May 15.

Department of Clinical Neurology, John Radcliffe Hospital, Oxford, UK.

Improvements in assays for detecting serum antibodies against myelin oligodendrocyte glycoprotein (MOG) have led to the appreciation of MOG-antibody-associated disease (MOGAD) as a novel disorder. However, much remains unknown about its etiology. We performed human leukocyte antigen (HLA) analysis in 82 MOGAD patients of European ancestry in the UK population. Read More

View Article and Full-Text PDF

A novel method for quantifying ingested food allergens in human sera.

Clin Exp Allergy 2021 May 15. Epub 2021 May 15.

Odense Research Center for Anaphylaxis (ORCA), Dept. of Dermatology & Allergy Center, Odense University Hospital, Odense, Denmark.

Severe reactions in IgE-mediated food-allergic patients can occur within minutes after ingestion, but the eliciting mechanism(s), linking the amount of allergenic food to the heterogeneity of clinical manifestations, are yet unknown. Quantification of IgE reactive allergenic food in circulation is a prerequisite for understanding the kinetics of the allergic immune response, and has been attempted based upon poly-/monoclonal antibody recognition of wheat , cow's milk , and peanut allergens. Read More

View Article and Full-Text PDF

Accurate and Reproducible Diagnosis of Peanut Allergy Using Epitope Mapping.

Allergy 2021 May 15. Epub 2021 May 15.

Department of Pediatrics, Allergy and Immunology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Background: Accurate diagnosis of peanut allergy is a significant clinical challenge. Here, a novel diagnostic blood test using the peanut Bead-Based Epitope Assay ("peanut BBEA") was developed utilizing the LEAP cohort and then validated using two independent cohorts.

Methods: The development of the peanut BBEA diagnostic test followed the National Academy of Medicine's established guidelines with discovery performed on 133 subjects from the non-interventional arm of the LEAP trial and an independent validation performed on 82 subjects from the CoFAR2 and 84 subjects from the POISED study. Read More

View Article and Full-Text PDF

Direct Comparison of Risankizumab and Fumaric Acid Esters in Systemic-Therapy-Naive Patients With Moderate to Severe Plaque Psoriasis: A Randomized Controlled Trial.

Br J Dermatol 2021 May 15. Epub 2021 May 15.

Dermatologikum, Hamburg, Germany.

Background: Fumaric acid esters (FAEs; Fumaderm ) are the most frequently prescribed first-line systemic treatment for moderate-to-severe plaque psoriasis in Germany. Risankizumab (Skyrizi ) is a humanized IgG1 monoclonal antibody that specifically binds to the p19 subunit of interleukin-23.

Objective: This study compared risankizumab treatment with FAEs in psoriasis patients. Read More

View Article and Full-Text PDF

Is the link between elevated TSH and gestational diabetes mellitus dependant on diagnostic criteria and thyroid antibody status: a systematic review and meta-analysis.

Endocrine 2021 May 15. Epub 2021 May 15.

School of Biomedical Sciences, The University of Queensland, Brisbane, QLD, Australia.

Purpose: Clinical studies have investigated the prevalence of gestational diabetes mellitus (GDM) in women with subclinical hypothyroidism (SCH). While some studies demonstrate a clear association, others do not. It is possible this may be due to varied diagnostic criteria for SCH and the presence of thyroid antibodies (TA). Read More

View Article and Full-Text PDF

First case of lethal encephalitis in Western Europe due to European bat lyssavirus type 1.

Clin Infect Dis 2021 May 15. Epub 2021 May 15.

Sorbonne Université, Brain Institute (ICM; INSERM, UMRS 1127; CNRS, UMR 7225), Paris, France.

Background: Inaccurate diagnosis of encephalitis is a major issue as immunosuppressive treatments can be deleterious in case of viral infection. The European bat lyssavirus type 1, a virus related to rabies virus, is endemic in European bats. No human case has yet been reported in Western Europe. Read More

View Article and Full-Text PDF

H3F3A-mutated Giant Cell Tumour of Bone without Giant Cells - Clinical Presentation, Radiology, and Histology of Three Cases.

Histopathology 2021 May 15. Epub 2021 May 15.

Bone Tumour Reference Center at the Institute of Pathology, University Hospital Basel, Basel, Switzerland.

Aims: Giant cell tumour of bone (GCTB) is histologically defined as a lesion containing reactive giant cells and a neoplastic mononuclear cell population; in up to 92% GCTB is characterized by a specific mutation of the histone gene H3F3A. The cellular composition ranges from giant cell-rich to giant cell-poor tumours. The diagnosis of GCTB can be challenging and several other lesions need to be excluded, e. Read More

View Article and Full-Text PDF

High-efficacy, high-manufacturability human VH domain antibody therapeutics from transgenic sources.

Protein Eng Des Sel 2021 Feb;34

Human Health Therapeutics Research Centre, Life Sciences Division, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada.

Interest in single-domain antibodies (sdAbs) stems from their unique structural/pronounced, hence therapeutically desirable, features. From the outset-as therapeutic modalities-human antibody heavy chain variable domains (VHs) attracted a particular attention compared with 'naturally-occurring' camelid and shark heavy-chain-only antibody variable domains (VHHs and VNARs, respectively) due to their perceived lack of immunogenicity. However, they have not quite lived up to their initial promise as the VH hits, primarily mined from synthetic VH phage display libraries, have too often been plagued with aggregation tendencies, low solubility and low affinity. Read More

View Article and Full-Text PDF
February 2021

Protein-based lateral flow assays for COVID-19 detection.

Protein Eng Des Sel 2021 Feb;34

Department of Chemical and Biomolecular Engineering New York University Tandon School of Engineering, Brooklyn, NY 11201, USA.

To combat the enduring and dangerous spread of COVID-19, many innovations to rapid diagnostics have been developed based on proteinprotein interactions of the SARS-CoV-2 spike and nucleocapsid proteins to increase testing accessibility. These antigen tests have most prominently been developed using the lateral flow assay (LFA) test platform which has the benefit of administration at point-of-care, delivering quick results, lower cost, and does not require skilled personnel. However, they have gained criticism for an inferior sensitivity. Read More

View Article and Full-Text PDF
February 2021

(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments.

Chemistry 2021 May 14. Epub 2021 May 14.

University Medical center Utrecht, Medical Microbiology, NETHERLANDS.

Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium  Staphylococcus aureus . WTAs are composed of repeating ribitol phosphate (RboP) residues that are decorated with  d -alanine and  N -acetyl- d -glucosamine (GlcNAc) modifications, in a seemingly random manner. These WTA-modifications play an important role in shaping the interactions of WTA with the host immune system. Read More

View Article and Full-Text PDF

Current situation of viral hepatitis in Egypt.

Microbiol Immunol 2021 May 15. Epub 2021 May 15.

Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.

An estimated 8-10 million people suffer from viral hepatitis in Egypt. Hepatitis A virus (HAV) and hepatitis E virus (HEV) are major causes of viral hepatitis in Egypt since ≥ 50% of the Egyptian population are already exposed to HAV infection by the age of 15 years. In addition, > 60% of the Egyptian population tested seropositive for anti-HEV in the first decade of life. Read More

View Article and Full-Text PDF