117,813 results match your criteria antibodies detected

Antithymocyte globulin is associated with a lower incidence of de novo donor-specific antibody detection in lung transplant recipients: A single-center experience.

Immun Inflamm Dis 2021 Jul 26. Epub 2021 Jul 26.

Department of Laboratory Medicine and Pathology, Mayo Clinic, Jacksonville, Florida, USA.

Purpose: Induction immunosuppression has improved the long-term outcomes after lung transplant. This is the first report exploring the association of induction immunosuppression with the development of de novo donor-specific human leukocyte antigen (HLA) antibodies (DSA) in lung transplant recipients (LTR).

Methods: Sixty-seven consecutive primary LTR were followed for 3 years posttransplant. Read More

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Humoral Response of Renal Transplant Recipients to the BNT162b2 SARS-CoV-2 mRNA Vaccine Using Both RBD IgG and Neutralizing Antibodies.

Transplantation 2021 Jul 6. Epub 2021 Jul 6.

Sackler Faculty of Medicine, Tel-Aviv University, Tel Aviv, Israel.

Background: Data about SARS-CoV-2 vaccines efficacy in renal transplant recipients (RTR) is lacking.

Methods: To reveal predictors for humoral response to BNT162b2 vaccine among RTR, patients were divided to positive (N=42) and negative (N=78) response groups based on receptor-binding domain (RBD) IgG >= 1.1 and neutralizing antibodies (NA) >= 16 dilution versus RBD IgG < 1. Read More

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Impact of SARS-CoV-2 variant-associated RBD mutations on the susceptibility to serum antibodies elicited by COVID-19 infection or vaccination.

Clin Infect Dis 2021 Jul 26. Epub 2021 Jul 26.

State Key Laboratory for Emerging Infectious Diseases, Carol Yu Centre for Infection, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People's Republic of China.

Background: Several SARS-CoV-2 lineages with mutations at the spike protein receptor binding domain (RBD) have reduced susceptibility to antibody neutralization, and have been classified as Variants of Concern (VOCs) or Variants of Interest (VOIs). Here, we systematically compared the neutralization susceptibility and RBD binding of different VOCs/VOIs, including B.1. Read More

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Application of biomarkers in the diagnosis of uncertain samples of core needle biopsy of thyroid nodules.

Virchows Arch 2021 Jul 26. Epub 2021 Jul 26.

Department of Pathology, Peking University First Hospital, 7 Xishiku Street, Xicheng District, Beijing, 100034, China.

Core needle biopsy (CNB) is now more frequently used for the preoperative diagnosis of thyroid nodules. Based on morphology alone, 5-20% of CNB samples cannot be determined as malignant or benign. Compared to fine-needle biopsy (FNB), samples collected by CNB are more accessible for various tests. Read More

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Factors Associated With IgG and IgM Antibodies, and Placental Histopathological Changes Among Women With Spontaneous Abortion in Mwanza City, Tanzania.

East Afr Health Res J 2017 1;1(2):86-94. Epub 2017 Jul 1.

Department of Medical Parasitology and Entomology, Weill Bugando School of Medicine, Mwanza, Tanzania.

Background: infection in early pregnancy has been associated with significant adverse pregnancy outcomes. Despite being common in the city of Mwanza, its association with spontaneous abortion has never been studied. Here, we report the IgG and IgM seropositivity and histopathological changes of toxoplasmosis among women with spontaneous abortion. Read More

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Serological detection and genetic characterization of foot-and-mouth disease virus from cattle in northern sudan, 2016‑2018.

Vet Anim Sci 2021 Sep 29;13:100188. Epub 2021 Jun 29.

Foot-and-Mouth-Disease Department, Central Veterinary Research Laboratory (CVRL), Soba, P.O. Box 8067, Al Amarat, Khartoum, Sudan.

Northern Sudan is an important corridor cluster between pools of foot-and-mouth disease virus (FMDV) in East and North Africa. It involves almost the whole border area with Egypt and represents a considerable part of a projected disease-free zone in Sudan. The study monitored FMD infection between 2016 and 2018 in Northern Sudan. Read More

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September 2021

Deep Sequencing of T-Cell Receptors for Monitoring Peripheral CD8 T Cells in Chinese Advanced Non-Small-Cell Lung Cancer Patients Treated With the Anti-PD-L1 Antibody.

Front Mol Biosci 2021 9;8:679130. Epub 2021 Jul 9.

Department of Medical Oncology, School of Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China.

Atezolizumab, a high-affinity engineered human anti-PD-L1 antibody, has produced a clinical benefit for patients with advanced non-small-cell lung cancer (NSCLC). However, associated with T-cell regulation, the immunomodulatory effect of PD-L1 blockade and its biomarker in peripheral immunity remains elusive. In a prospective cohort with 12 Chinese advanced NSCLC patients who received atezolizumab 1,200 mg every 3 weeks as a second-line treatment, blood samples were obtained before and 6 weeks after atezolizumab initiation, and when disease progression was confirmed. Read More

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First seroprevalence investigation of epizootic haemorrhagic disease virus in Libya.

Open Vet J 2021 Apr-Jun;11(2):301-308. Epub 2021 Jun 21.

Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", (IZSAM), Teramo, Italy.

Background: Epizootic haemorrhagic disease (EHD) is a vector-borne viral disease of domestic and wild ruminants. Epizootic haemorrhagic disease virus (EHDV) is transmitted by spp. EHDV is a member of the genus within the family. Read More

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Clinical value analysis of IgM and IgG antibodies detected by nucleic acid in patients with COVID-19.

Am J Transl Res 2021 15;13(6):7089-7103. Epub 2021 Jun 15.

Department of Hepatobiliary Surgery, Hanchuan People's Hospital Hanchuan, Hubei Province, China.

Objective: We aimed to analyze the clinical diagnostic value of nucleic acid detection and specific IgM and IgG antibodies in COVID-19 patients. According to the patients' test outcomes of nucleic acid and specific IgM and IgG antibodies, the patients were grouped.

Methods: Medical records of 788 COVID-19 patients were collected for retrospective analysis, including demographic data, clinical characteristics, CT outcome and laboratory indicators. Read More

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Evaluation of the diagnostic potential and DIVA capability of recombinant LigBCon1-5 protein of serovar Pomona in canine leptospirosis.

Iran J Vet Res 2021 ;22(2):120-128

Ph.D. Student in Biotechnology, Institute of Chemistry, Academia Sinica University, Nankang, Taipei, Taiwan.

Background: Canine leptospirosis is a serious public health concern.

Aims: This study aims to investigate the feasibility of conserved first to fifth domains of recombinant immunoglobulin like protein B antigen (rLigBCon1-5) as a serodiagnostic marker for detecting canine leptospirosis.

Methods: A total of 340 unvaccinated canine serum samples were screened using microscopic agglutination test (MAT) and rLigBCon1-5 based immunoglobulin G (IgG) indirect-enzyme-linked immunosorbent assay (I-ELISA). Read More

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January 2021

Pathogenic Role of Circulating Citrullinated Antigens and Anti-Cyclic Monoclonal Citrullinated Peptide Antibodies in Rheumatoid Arthritis.

Front Immunol 2021 30;12:692242. Epub 2021 Jun 30.

Clinical Immunology and Stem Cell Laboratory (CiSTEM) Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine, The Catholic University of Korea, Seoul, South Korea.

We examined whether it is possible to directly detect citrullinated antigens in the serum of rheumatoid arthritis (RA) patients using a monoclonal antibody (mAb) designed to be specific for citrullinated peptides. In order to confirm the potential of the mAb as a direct arthritis-inducing substance through experimental model of RA, a monoclonal antibody (mAb) 12G1 was generated using by immunization of mice with a challenging cyclic citrullinated peptide. Immunohistochemical analysis of RA-affected synovial tissue showed that our mAb 12G1 could indeed detect citrullinated proteins in target tissues. Read More

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The Effect of Immunosuppressive Adjuvant Kynurenine on Type 1 Diabetes Vaccine.

Front Immunol 2021 7;12:681328. Epub 2021 Jul 7.

Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.

Inducing antigen-specific tolerance is a promising treatment for preventing or reversing Type 1 diabetes (T1D). In contrast to a vaccine that induces immune responses against pathogens, a tolerogenic vaccine can suppress immunity against antigens causing diseases by administrating a mixture of self-antigens with an adjuvant that decreases the strength of antigen-specific response. Kynurenine (Kyn) is an endogenous substance that can inhibit the natural killer cell and T cell proliferation and promote the differentiation of naïve T cells into regulatory T cells (T). Read More

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Comparative Analysis of the Measles Antibody Levels in Healthy Medical Personnel of Maternity Ward and Women in Labor.

Front Immunol 2021 8;12:680506. Epub 2021 Jul 8.

Laboratory of Vaccine Prophylaxis and Immunotherapy of Allergic Diseases of I.I.Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.

It has been proven that post-vaccination immunity to measles virus after two doses of vaccine is not able to persistently protect against infection throughout life. The goal of this research was to determine the immune layer to the measles virus among women in labor and maternity ward personnel in the same medical institution. The levels of IgG antibodies to measles virus in the umbilical cord blood of 594 women in labor and 88 workers of the maternity ward were studied by ELISA. Read More

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Seroprevalence of Hepatitis A and Hepatitis E Viruses among Pregnant Women in Northern Iran.

Infect Dis Obstet Gynecol 2021 2;2021:5130586. Epub 2021 Jul 2.

Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Background: Hepatitis A (HAV) and hepatitis E viruses (HEV) are endemic in Iran and are known major causes of acute viral hepatitis. Also, during pregnancy, they are associated with severe outcomes. Therefore, it is vital to evaluate the antibody levels against HAV and HEV in pregnant women to avoid severe outcomes incidence. Read More

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[Immunogenicity and safety of concomitant inoculation of Measles and rubella combined live vaccine and Japanese encephalitis attenuated live vaccine].

Zhonghua Yu Fang Yi Xue Za Zhi 2021 Jul;55(7):835-839

Expanded Program Immunization Department of Zhejiang Provincial Center for Diseases Control and Prevention, Hangzhou 310051, China.

To evaluate the immunogenicity and safety of concomitant inoculation of Measles and Rubella Combined Live-attenuated Japanese encephalitis vaccine. Healthy children aged 8 months in selected cities in Zhejiang Province were randomly assigned to the MR and JVE-L combined vaccination group (the experimental group), and the MR single vaccination group (the control group) was taken before the exemption and 6 weeks after the exemption. Peripheral venous blood 1 ml was used as serum antibody test for measles and rubella, and the levels of measles and rubella antibodies before and after vaccination were evaluated. Read More

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Survival patterns of Streptococcus suis serotypes 1 and 14 in porcine blood indicate cross-reactive bactericidal antibodies in naturally infected pigs.

Vet Microbiol 2021 Jul 16;260:109183. Epub 2021 Jul 16.

Institute of Bacteriology and Mycology, Centre for Infectious Diseases, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany. Electronic address:

Streptococcus suis serotype (cps) 1 and cps14 have been detected in association with severe diseases such as meningitis and polyarthritis in pigs. Though these two cps are very similar, only cps14 is an important zoonotic agent in Asia and only cps1 is described to be associated with diseases in suckling piglets rather than weaning piglets. The main objective of this study was to assess restriction of survival of cps14 and cps1 in porcine blood by IgG and IgM putatively cross-reacting with these two cps. Read More

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A study of rural chicken farmers, diseases and remedies in the Eastern Cape province of South Africa.

Prev Vet Med 2021 Jul 12;194:105430. Epub 2021 Jul 12.

Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa; Discipline of Veterinary Sciences, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, 4811, Australia.

The source of emerging diseases and antimicrobial resistance is of increasing interest to epidemiologists. This paper looks at village chickens as such a source. In addition, infectious diseases constitute a major challenge to the growth and profitability of the rural poultry sector in Sub-Saharan Africa. Read More

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Evaluation of Shear Horizontal Surface Acoustic Wave Biosensors Using "Layer Parameter" Obtained from Sensor Responses during Immunoreaction.

Sensors (Basel) 2021 Jul 20;21(14). Epub 2021 Jul 20.

Graduate School of Science and Technology, Shizuoka University, Shizuoka 432-8561, Japan.

Shear horizontal surface acoustic wave (SH-SAW) biosensors measure the reaction of capture antibodies immobilized on the sensing surface to capture test molecules (antigens) by using the change in SH-SAW propagation characteristics. SH-SAW displacement exists not only on the SH-SAW propagating surface, but also partially penetrates the specimen liquid to a certain depth, which is determined by the liquid properties of the specimen and the operating frequency of the SH-SAW. This phenomenon is called viscosity penetration. Read More

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Specific Increase in Joint Neutrophil Extracellular Traps and Its Relation to Interleukin 6 in Autoimmune Arthritis.

Int J Mol Sci 2021 Jul 16;22(14). Epub 2021 Jul 16.

Division of Rheumatology, Department of Internal Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.

Neutrophils and their extracellular traps have been shown to play an important role in the pathogenesis of rheumatoid arthritis (RA), but the detailed mechanisms in joints are still unclear, and their regulation remains to be solved. Here, we explored neutrophil extracellular trap (NET)osis in experimental models of arthritis and further investigated the effects of interleukin-6 (IL-6) inhibition in neutrophils and NETosis. In skins of peptide GPI-induced arthritis (pGIA), citrullinated protein was detected as well as citrullinated histone expression in immunized skin but this was not specific to pGIA. Read More

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Noggin, an inhibitor of bone morphogenetic protein signaling, antagonizes TGF-β1 in a mouse model of osteoarthritis.

Biochem Biophys Res Commun 2021 Jul 20;570:199-205. Epub 2021 Jul 20.

Department of Orthopaedic Surgery, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China; Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. Electronic address:

Osteoarthritis (OA) is the most common joint disease worldwide; however, disease-modifying treatments are lacking because of the complicated pathological mechanisms. As a breakthrough, aberrant activation of transforming growth factor-β 1 (TGF-β1)in subchondral bone has been confirmed as an essential pathomechanism for OA progression, and has become a potential therapeutic target. In addition to R&D on neutralizing antibodies, small-molecule antagonists and chemical medicines, native antagonists of TGF-β1 could be exploited as another promising approach. Read More

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A three-phase population based sero-epidemiological study: Assessing the trend in prevalence of SARS-CoV-2 during COVID-19 pandemic in Jordan.

One Health 2021 Dec 10;13:100292. Epub 2021 Jul 10.

Jordan University Hospital, Amman, Jordan.

The evolution of the COVID-19 pandemic in Jordan during the first 10 months of the epidemic was peculiar and can be easily categorized in three different phases: a first period featuring a very low number of reported cases, a second period with exponential growth from August with up to 8000 cases on the 18th November 2020, and a third phase with steady and progressive decline of the epidemiological curve. With the aim of better determine the entity of the population exposed to SARS-CoV-2, the Jordan Ministry of Health with the support of the WHO launched three rounds of the nationwide sero-prevalence survey. Using population proportionate to size (PPS) methodology, around 5000 individuals were selected from all Jordan governorates. Read More

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December 2021

Achieving High Poliovirus Antibody Seroprevalence in Areas at Risk of Vaccine-Derived Poliovirus Transmission-Niger Experience.

Open Forum Infect Dis 2021 Jul 6;8(7):ofab210. Epub 2021 May 6.

Polio Eradication Department, World Health Organization, Geneva, Switzerland.

Background: Outbreaks of vaccine-derived poliovirus type 2 (VDPV2) continue to expand across Africa. We conducted a serological survey of polio antibodies in high-polio risk areas of Niger to assess risk of poliovirus outbreaks.

Methods: Children between 1 and 5 years of age were enrolled from structures randomly selected using satellite imaging enumeration in Diffa Province, Niger, in July 2019. Read More

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Seroprevalence of spp. in children (3-13 years old) in Zahedan, Southeast of Iran.

J Parasit Dis 2021 Jun 26;45(2):449-453. Epub 2020 Nov 26.

Infectious Disease and Tropical Medicine Research Center, Resistance Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran.

Child Toxocariasis (CT) is one of the most important helminthic parasitic diseases in Iran. The current study was aimed to determine the seroprevalence of toxocariasis in children in Zahedan, southeast of Iran. In this cross-sectional study, serum samples of 373 children aged 3-13 years old referred to health centers in Zahedan were collected randomly with criteria based on the parameters that were used in earlier studies and examined for anti-Toxocara antibody using a commercial ELISA kit. Read More

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A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19.

NPJ Vaccines 2021 Jul 22;6(1):91. Epub 2021 Jul 22.

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.

The development of effective countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the agent responsible for the COVID-19 pandemic, is a priority. We designed and produced ConVac, a replication-competent vesicular stomatitis virus (VSV) vaccine vector that expresses the S1 subunit of SARS-CoV-2 spike protein. We used golden Syrian hamsters as animal models of severe COVID-19 to test the efficacy of the ConVac vaccine. Read More

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The Clinicopathological Distinction Between Seropositive and Seronegative Immune-Mediated Necrotizing Myopathy in China.

Front Neurol 2021 5;12:670784. Epub 2021 Jul 5.

Department of Neurology, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.

The present study aimed to compare the clinicopathological features of patients with seronegative immune-mediated necrotizing myopathy (IMNM) and those positive for anti-signal recognition particle (SRP) or anti-3-hydroxy-3-methylglutarylcoenzyme-a reductase (HMGCR) antibodies. We retrospectively analyzed the data of patients with IMNM treated in the Neurology Department of Tongji Hospital from January 1, 2013, to December 31, 2019. Among the 117 patients with IMNM, 30. Read More

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Kinetics and seroprevalence of SARS-CoV-2 antibodies: a comparison of 3 different assays.

Sci Rep 2021 07 21;11(1):14893. Epub 2021 Jul 21.

Department of Pediatrics, University Hospital and Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstrasse 74, 01307, Dresden, Germany.

Comparing seroprevalence and antibody kinetics in three different commercially available assays for SARS-CoV-2. Serostatus of COVID-19 patients was analyzed 5 months and 10 months after their infection, using three different assays: Diasorin LIAISON, Euroimmun, Abbott Diagnostics ARCHITECT. Seropositivity at baseline differed significantly depending on the assay (Diasorin 81%, Euroimmun 83%, Abbott 59%). Read More

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Association Between COVID-19 Exposure and Self-reported Compliance With Public Health Guidelines Among Essential Employees at an Institution of Higher Education in the US.

JAMA Netw Open 2021 07 1;4(7):e2116543. Epub 2021 Jul 1.

Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins.

Importance: Detailed analysis of infection rates paired with behavioral and employee-reported risk factors is vital to understanding how transmission of SARS-CoV-2 infection may be exacerbated or mitigated in the workplace. Institutions of higher education are heterogeneous work units that supported continued in-person employment during the COVID-19 pandemic, providing a test site for occupational health evaluation.

Objective: To evaluate the association between self-reported protective behaviors and prevalence of SARS-CoV-2 infection among essential in-person employees during the first 6 months of the COVID-19 pandemic in the US. Read More

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Prevalence of active HCV infection and genotypic distribution among the general population of district Mardan, Pakistan.

Braz J Biol 2021 14;83:e244977. Epub 2021 Jul 14.

Lanzhou University, School of Life Sciences, MOE Key Laboratory of Cell Activities and Stress Adaptations, Lanzhou, Gansu, China.

Hepatitis C virus (HCV) is the serious global public health burden of liver disease. Approximately 170 million people in the world are infected with (HCV). In Pakistan, where the disease has high occurrence rate. Read More

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Participatory and Transdisciplinary Studies of Infection in Humans and Animals in Yunnan Province, China-Lessons Learned.

Trop Med Infect Dis 2021 Jul 15;6(3). Epub 2021 Jul 15.

International Livestock Research Institute, Hanoi 100000, Vietnam.

Brucellosis is an important zoonosis occurring globally. In addition to the risk for disease in humans, the disease causes production losses, since the disease in livestock is characterized by abortion and other reproductive failures. The disease is a public health concern in China, but no information is available on knowledge, perception and awareness of potential risk groups such as farmers, butchers and animal health workers; yet successful control requires compliance of those affected groups to be effective. Read More

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Durable Antibody Responses in Staff at Two Long-Term Care Facilities, during and Post SARS-CoV-2 Outbreaks.

Microbiol Spectr 2021 Jul 21:e0022421. Epub 2021 Jul 21.

Department of Microbiology, Immunology and Pathology, Colorado State Universitygrid.47894.36, Fort Collins, Colorado, USA.

SARS-CoV-2 has had a disproportionate impact on nonhospital health care settings, such as long-term-care facilities (LTCFs). The communal nature of these facilities, paired with the high-risk profile of residents, has resulted in thousands of infections and deaths and a high case fatality rate. To detect presymptomatic infections and identify infected workers, we performed weekly surveillance testing of staff at two LTCFs, which revealed a large outbreak at one of the sites. Read More

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