6,009 results match your criteria antibiotics substrates

Poultry litter stabilization by two-stage composting-vermicomposting process: Environmental, energetic and economic performance.

Chemosphere 2021 Oct 15;281:130872. Epub 2021 May 15.

Instituto de Desarrollo Tecnológico para la Industria Química (INTEC, UNL-CONICET), Güemes N(◦) 3450, Santa Fe, S3000GLN, Argentina; Facultad de Ingeniería y Ciencias Hídricas, Universidad Nacional del Litoral, Ruta Nacional N° 168 Km 472.4, 3000, Santa Fe, Argentina.

Poultry litter (PL) is a heterogeneous mixture that contains bedding materials, antibiotics, dead skin, feed scraps, water, feathers and the resulting microbiota from poultry production cycle. Although its treatment does not receive attention, it is an important organic resource generated in the north-east region of Santa Fe Province since animal primary production is one of the main economic activities there and in the whole country. The objective of this work was to analyse the economic and energy aspects and the environmental impact of two scenarios corresponding to treat two different mixtures of organic wastes: Eucalyptus sp. Read More

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October 2021

Protein Transfer through an F Plasmid-Encoded Type IV Secretion System Suppresses the Mating-Induced SOS Response.

mBio 2021 Jul 13:e0162921. Epub 2021 Jul 13.

Department of Microbiology and Molecular Genetics, McGovern Medical School, Houston, Texas, USA.

Bacterial type IV secretion systems (T4SSs) mediate the conjugative transfer of mobile genetic elements (MGEs) and their cargoes of antibiotic resistance and virulence genes. Here, we report that the pED208-encoded T4SS (Tra) translocates not only this F plasmid but several plasmid-encoded proteins, including ParA, ParB1, single-stranded DNA-binding protein SSB, ParB2, PsiB, and PsiA, to recipient cells. Conjugative protein translocation through the Tra T4SS required engagement of the pED208 relaxosome with the TraD substrate receptor or coupling protein. Read More

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The G132S Mutation Enhances the Resistance of β-Lactamase against Sulbactam.

Biochemistry 2021 Jul 12;60(28):2236-2245. Epub 2021 Jul 12.

Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333CC Leiden, The Netherlands.

The current rise of antibiotic resistant forms of is a global health threat that calls for new antibiotics. The β-lactamase BlaC of this pathogen prevents the use of β-lactam antibiotics, except in combination with a β-lactamase inhibitor. To understand if exposure to such inhibitors can easily result in resistance, a BlaC evolution experiment was performed, studying the evolutionary adaptability against the inhibitor sulbactam. Read More

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Twin-arginine translocation (Tat) mutants in serovar Typhimurium have increased susceptibility to cell wall targeting antibiotics.

FEMS Microbes 2021 14;2:xtab004. Epub 2021 Apr 14.

Department of Microbiology and Immunology, Midwestern University, 19555 N 59th Avenue, Glendale, AZ 85308, USA.

The twin-arginine translocation (Tat) system is a protein secretion system that is conserved in bacteria, archaea and plants. In Gram-negative bacteria, it is required for the export of folded proteins from the cytoplasm to the periplasm. There are 30 experimentally verified Tat substrates in , including hydrogenase subunits, enzymes required for anaerobic respiration and enzymes involved in peptidoglycan remodeling during cell division. Read More

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[Application of methyltransferases in microbial synthesis of natural products].

Sheng Wu Gong Cheng Xue Bao 2021 Jun;37(6):1869-1886

College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

Methyltransferases (MTs) constitute a large group of enzymes that catalyze the transfer of a methyl moiety, most frequently from S-adenosyl-L-methionine, to their substrates. It plays an essential role in regulation of gene expression and synthesis of many natural compounds. Owing to its broad substrate spectrum, MTs make important contributions to diversify the spectrum of products through methylation modifications. Read More

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Exploration of inhibition potential of isoniazid and its metabolites towards CYP2E1 in human liver microsomes through LC-MS/MS analysis.

J Pharm Biomed Anal 2021 Sep 24;203:114223. Epub 2021 Jun 24.

Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), S.A.S Nagar, 160062, Punjab, India. Electronic address:

Isoniazid (INH) is the first-line anti-tubercular drug that is used both for the prophylaxis as well as the treatment of tuberculosis (TB). The patients with TB are more vulnerable to secondary infections and other health complications, hence, they are usually administered a cocktail of drugs. This increases the likelihood of drug-drug interactions (DDIs). Read More

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September 2021

Antimicrobial Peptides from Black Soldier Fly () as Potential Antimicrobial Factors Representing an Alternative to Antibiotics in Livestock Farming.

Animals (Basel) 2021 Jun 29;11(7). Epub 2021 Jun 29.

Research Center for Environmental Ecology and Engineering, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430073, China.

Functional antimicrobial peptides (AMPs) are an important class of effector molecules of innate host immune defense against pathogen invasion. Inability of microorganisms to develop resistance against the majority of AMPs has made them alternatives to antibiotics, contributing to the development of a new generation of antimicrobials. Due to extensive biodiversity, insects are one of the most abundant sources of novel AMPs. Read More

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The Intestinal Microbiota: Impacts of Antibiotics Therapy, Colonization Resistance, and Diseases.

Int J Mol Sci 2021 Jun 20;22(12). Epub 2021 Jun 20.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.

Trillions of microbes exist in the human body, particularly the gastrointestinal tract, coevolved with the host in a mutually beneficial relationship. The main role of the intestinal microbiome is the fermentation of non-digestible substrates and increased growth of beneficial microbes that produce key antimicrobial metabolites such as short-chain fatty acids, etc., to inhibit the growth of pathogenic microbes besides other functions. Read More

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Antimicrobial Peptide Dendrimers and Quorum-Sensing Inhibitors in Formulating Next-Generation Anti-Infection Cell Therapy Dressings for Burns.

Molecules 2021 Jun 24;26(13). Epub 2021 Jun 24.

Department of Microbiology and Molecular Medicine, University of Geneva, 1211 Geneva, Switzerland.

Multidrug resistance infections are the main cause of failure in the pro-regenerative cell-mediated therapy of burn wounds. The collagen-based matrices for delivery of cells could be potential substrates to support bacterial growth and subsequent lysis of the collagen leading to a cell therapy loss. In this article, we report the development of a new generation of cell therapy formulations with the capacity to resist infections through the bactericidal effect of antimicrobial peptide dendrimers and the anti-virulence effect of anti-quorum sensing MvfR (PqsR) system compounds, which are incorporated into their formulation. Read More

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Ruthenium Complexes in the Fight against Pathogenic Microorganisms. An Extensive Review.

Pharmaceutics 2021 Jun 13;13(6). Epub 2021 Jun 13.

Department of General and Inorganic Chemistry, Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy, 020956 Bucharest, Romania.

The widespread use of antibiotics has resulted in the emergence of drug-resistant populations of microorganisms. Clearly, one can see the need to develop new, more effective, antimicrobial agents that go beyond the explored 'chemical space'. In this regard, their unique modes of action (e. Read More

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Effectors of the Stenotrophomonas maltophilia Type IV Secretion System Mediate Killing of Clinical Isolates of Pseudomonas aeruginosa.

mBio 2021 06 29;12(3):e0150221. Epub 2021 Jun 29.

Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, Illinois, USA.

Previously, we documented that Stenotrophomonas maltophilia encodes a type IV secretion system (T4SS) that allows the organism to kill, in contact-dependent fashion, heterologous bacteria, including wild-type Pseudomonas aeruginosa. Bioinformatic screens based largely on the presence of both a C-terminal consensus sequence and an adjacent gene encoding a cognate immunity protein identified 13 potential antibacterial effectors, most of which were highly conserved among sequenced strains of S. maltophilia. Read More

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Rapid Biocidal Activity of -Halamine-Functionalized Polydopamine and Polyethylene Imine Coatings.

Langmuir 2021 07 23;37(26):8037-8044. Epub 2021 Jun 23.

Department of Chemical and Materials Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 807618, Taiwan.

Microorganisms easily adhere to the surface of substrates and further form biofilms, which present problems in various fields. Therefore, the development of surfaces with antimicrobial adhesion or viability is a promising approach. In this study, we were committed to develop a rapid sterilizing coating. Read More

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Leveraging Physiologically-Based Pharmacokinetic (PBPK) Modeling and Experimental Data to Guide Dosing Modification of CYP3A-Mediated Drug-Drug Interactions (DDIs) in the Pediatric Population.

Drug Metab Dispos 2021 Jun 21. Epub 2021 Jun 21.

UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, United States

Solithromycin is a novel fluoroketolide antibiotic that is both a substrate and time-dependent inhibitor of cytochrome P450 (CYP) 3A. Solithromycin has demonstrated efficacy in adults with community-acquired bacterial pneumonia (CABP) and has also been investigated in pediatric patients. The objective of this study was to develop a framework for leveraging physiologically-based pharmacokinetic (PBPK) modeling to predict CYP3A-mediated drug-drug interaction (DDI) potential in the pediatric population using solithromycin as a case study. Read More

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In situ preparation of porous metal-organic frameworks [email protected] on poly-ether-ether-ketone with synergistic antibacterial activity.

Colloids Surf B Biointerfaces 2021 Sep 11;205:111920. Epub 2021 Jun 11.

Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, School of Materials and Energy, Southwest University, Chongqing, 400715, PR China. Electronic address:

Poly-ether-ether-ketone (PEEK) is a promising material in oral repair and orthopedic implantation field due to its stability and proper elastic modulus. However, the lack of simple but effective strategy to functionalize PEEK and improve its antibacterial function hinders its further biomedical application. In this study, a sulfonated 3D porous PEEK is fabricated via sulfonation treatment, and then decorated with the in situ synthesized zeolitic imidazolate framework-8 (ZIF-8), in which Ag ions were loaded with high loading capacity. Read More

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September 2021

Impact of Urbanization on Antibiotic Resistome in Different Microplastics: Evidence from a Large-Scale Whole River Analysis.

Environ Sci Technol 2021 07 16;55(13):8760-8770. Epub 2021 Jun 16.

Key Laboratory of Urban Environment and Health, Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.

Microplastics (MPs) are becoming ubiquitous in environments and viewed as carriers of antibiotic resistance genes (ARGs). Rivers connecting differently urbanized areas contribute a significant input of MPs and ARGs to the environment. However, a systematic study assessing the role of urbanization in shaping antibiotic resistome and mobilome in riverine MPs is lacking. Read More

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Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.

Chem Rev 2021 Jul 15;121(13):7957-8094. Epub 2021 Jun 15.

Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Universidad Nacional de Rosario, Ocampo y Esmeralda S/N, 2000 Rosario, Argentina.

Antimicrobial resistance is one of the major problems in current practical medicine. The spread of genes coding for resistance determinants among bacteria challenges the use of approved antibiotics, narrowing the options for treatment. Resistance to carbapenems, last resort antibiotics, is a major concern. Read More

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Facile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings.

Sci Rep 2021 06 14;11(1):12410. Epub 2021 Jun 14.

Department of Chemical and Biological Engineering and Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, 12180, USA.

In situ generation of antibacterial and antiviral agents by harnessing the catalytic activity of enzymes on surfaces provides an effective eco-friendly approach for disinfection. The perhydrolase (AcT) from Mycobacterium smegmatis catalyzes the perhydrolysis of acetate esters to generate the potent disinfectant, peracetic acid (PAA). In the presence of AcT and its two substrates, propylene glycol diacetate and HO, sufficient and continuous PAA is generated over an extended time to kill a wide range of bacteria with the enzyme dissolved in aqueous buffer. Read More

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Preparation of a bacterial flocculant by using caprolactam as a sole substrate and its application in amoxicillin removal.

J Environ Manage 2021 Sep 10;294:113026. Epub 2021 Jun 10.

Hubei Key Laboratory of Purification and Application of Plant Anti-Cancer Active Ingredients, Chemistry and Biology Science College, Hubei University of Education, Wuhan, 430205, China. Electronic address:

High cost is one of the limiting factors in the industrial production of bioflocculant. Simultaneous preparation of bioflocculant from the contaminants in wastewater was considered as a potential approach to reduce the production cost. In this study, caprolactam was verified as sole feedstock for the growth of strain Alcaligenes faecalis subsp. Read More

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September 2021

Reprogramming of the Caseinolytic Protease by ADEP Antibiotics: Molecular Mechanism, Cellular Consequences, Therapeutic Potential.

Front Mol Biosci 2021 13;8:690902. Epub 2021 May 13.

Microbial Bioactive Compounds, Interfaculty Institute of Microbiology and Infection Medicine, University of Tuebingen, Tübingen, Germany.

Rising antibiotic resistance urgently calls for the discovery and evaluation of novel antibiotic classes and unique antibiotic targets. The caseinolytic protease Clp emerged as an unprecedented target for antibiotic therapy 15 years ago when it was observed that natural product-derived acyldepsipeptide antibiotics (ADEP) dysregulated its proteolytic core ClpP towards destructive proteolysis in bacterial cells. A substantial database has accumulated since on the interaction of ADEP with ClpP, which is comprehensively compiled in this review. Read More

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The repertoire of ABC proteins in .

Comput Struct Biotechnol J 2021 8;19:2905-2920. Epub 2021 May 8.

Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.

ATP-binding cassette (ABC) transporters belong to one of the largest membrane protein superfamilies, which function in translocating substrates across biological membranes using energy from ATP hydrolysis. Currently, the classification of ABC transporters in is not complete. Therefore, the sequence-function relationship of all ABC proteins encoded within the genome was analyzed. Read More

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Fate of antibiotics and antibiotic resistance genes during aerobic co-composting of food waste with sewage sludge.

Sci Total Environ 2021 Aug 7;784:146950. Epub 2021 Apr 7.

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, People's Republic of China. Electronic address:

Aerobic composting is widely used on transforming organic solid waste into proliferating products. However, the removal of antibiotics and antibiotic resistance genes (ARGs) in the process of co-composting of food waste with sewage sludge has been rarely reported to date. Therefore, we investigated a laboratory-scale composting using food waste and sewage sludge as substrates to study changes in antibiotics and ARGs during composting. Read More

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Investigation on the uptake of ciprofloxacin, chloramphenicol and praziquantel by button mushrooms.

Food Chem 2021 Nov 13;362:130092. Epub 2021 May 13.

German Federal Institute for Risk Assessment, Department Safety in the Food Chain, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany. Electronic address:

Button mushrooms are widely produced edible basidiomycetes. Commercially, they are cultivated on substrates containing fermented horse manure and chicken feces. Since pharmacologically active substances (PAS) might be introduced into the food chain via animal treatment, their residues may be present in manure used for mushroom growth. Read More

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November 2021

How anammox responds to the emerging contaminants: Status and mechanisms.

J Environ Manage 2021 Sep 1;293:112906. Epub 2021 Jun 1.

School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, China. Electronic address:

Numerous researches have been carried out to study the effects of emerging contaminants in wastewater, such as antibiotics, nanomaterials, heavy metals, and microplastics, on the anammox process. However, they are fragmented and difficult to provide a comprehensive understanding of their effects on reactor performance and the metabolic mechanisms in anammox bacteria. Therefore, this paper overviews the effects on anammox processes by the introduced emerging contaminants in the past years to fulfill such knowledge gaps that affect our perception of the inhibitory mechanisms and limit the optimization of the anammox process. Read More

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September 2021

Mix-and-Match System for the Enzymatic Synthesis of Enantiopure Glycerol-3-Phosphate-Containing Capsule Polymer Backbones from , , and .

mBio 2021 06 26;12(3):e0089721. Epub 2021 May 26.

Institute of Clinical Biochemistry, Hannover Medical School, Hannover, Germany.

Capsule polymers are crucial virulence factors of pathogenic bacteria and are used as antigens in glycoconjugate vaccine formulations. Some Gram-negative pathogens express poly(glycosylglycerol phosphate) capsule polymers that resemble Gram-positive wall teichoic acids and are synthesized by TagF-like capsule polymerases. So far, the biotechnological use of these enzymes for vaccine developmental studies was restricted by the unavailability of enantiopure CDP-glycerol, one of the donor substrates required for polymer assembly. Read More

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Bacteriostatic Behavior of PLA-BaTiO Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging Test.

Molecules 2021 May 14;26(10). Epub 2021 May 14.

Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan.

The present work investigated the effect of Polylactic acid (PLA) fibers produced by centrifugal spinning with incorporated BaTiO particles to improve their bacteriostatic behavior. The PLA matrix and three composites, presenting three different amounts of fillers, were subjected to UV/O treatment monitoring the possible modifications that occurred over time. The morphological and physical properties of the surfaces were characterized by different microscopic techniques, contact angle, and surface potential measurements. Read More

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L. Leaf: A Systematic Scoping Review on Biological Safety and Herb-Drug Interactions.

Evid Based Complement Alternat Med 2021 7;2021:5511221. Epub 2021 May 7.

Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health Malaysia, Setia Alam 40170, Shah Alam, Malaysia.

Introduction: The L. leaf is gaining interest as a potential therapeutic agent for alleviating dengue- and non-dengue-associated thrombocytopaenia. In that regard, safety considerations are as important as efficacy potential. Read More

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Strategies for Surface Immobilization of Whole Bacteriophages: A Review.

ACS Biomater Sci Eng 2021 06 26;7(6):1987-2014. Epub 2021 May 26.

Université Grenoble Alpes, CNRS, CEA, IRIG, SyMMES, 38000 Grenoble, France.

Bacteriophage immobilization is a key unit operation in emerging biotechnologies, enabling new possibilities for biodetection of pathogenic microbes at low concentration, production of materials with novel antimicrobial properties, and fundamental research on bacteriophages themselves. Wild type bacteriophages exhibit extreme binding specificity for a single species, and often for a particular subspecies, of bacteria. Since their specificity originates in epitope recognition by capsid proteins, which can be altered by chemical or genetic modification, their binding specificity may also be redirected toward arbitrary substrates and/or a variety of analytes in addition to bacteria. Read More

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Local interactions with the Glu166 base and the conformation of an active site loop play key roles in carbapenem hydrolysis by the KPC-2 β-lactamase.

J Biol Chem 2021 Jan-Jun;296:100799. Epub 2021 May 20.

Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas, USA; Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston Texas, USA. Electronic address:

The Klebsiella pneumoniae carbapenemase-2 (KPC-2) is a common source of antibiotic resistance in Gram-negative bacterial infections. KPC-2 is a class A β-lactamase that exhibits a broad substrate profile and hydrolyzes most β-lactam antibiotics including carbapenems owing to rapid deacylation of the covalent acyl-enzyme intermediate. However, the features that allow KPC-2 to deacylate substrates more rapidly than non-carbapenemase enzymes are not clear. Read More

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evaluation of intestinal human CYP3A inhibition by macrolide antibiotics in CYP3A-humanised mice.

Xenobiotica 2021 Jul 20;51(7):764-770. Epub 2021 May 20.

Department of Biopharmaceutics, Graduate School of Clinical Pharmacy, Meiji Pharmaceutical University, Tokyo, Japan.

It is important to predict drug-drug interactions inhibition of intestinal cytochrome P450 3A (CYP3A) which is a determinant of bioavailability of orally administered CYP3A substrates. However, inhibitory effects of macrolide antibiotics on CYP3A-mediated metabolism are not entirely identical between humans and rodents.We investigated the effects of macrolide antibiotics, clarithromycin and erythromycin, on and metabolism of triazolam, a CYP3A substrate, in CYP3A-humanised mice generated by using a mouse artificial chromosome vector carrying a human gene. Read More

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Biochemical characterization of OXA-244, an emerging OXA-48 variant with reduced β-lactam hydrolytic activity.

J Antimicrob Chemother 2021 Jul;76(8):2024-2028

Team ReSIST, INSERM U1184, School of Medicine Université Paris-Saclay, LabEx LERMIT, and Joint Research Unit EERA 'Evolution and Ecology of Resistance to Antibiotics', Institut Pasteur-APHP-University Paris Saclay, Le Kremlin-Bicêtre, France.

Background: OXA-48-producing Enterobacterales have widely disseminated globally with an increasing number of variants identified. Among them, OXA-244 is increasingly reported, despite detection difficulties.

Objectives: To determine the steady-state kinetic parameters of OXA-244. Read More

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