591 results match your criteria antibiotics straightforward

Interbase-FRET binding assay for pre-microRNAs.

Sci Rep 2021 Apr 30;11(1):9396. Epub 2021 Apr 30.

Department of Chemistry and Molecular Biology, University of Gothenburg, 412 96, Gothenburg, Sweden.

The aberrant expression of microRNAs (miRs) has been linked to several human diseases. A promising approach for targeting these anomalies is the use of small-molecule inhibitors of miR biogenesis. These inhibitors have the potential to (i) dissect miR mechanisms of action, (ii) discover new drug targets, and (iii) function as new therapeutic agents. Read More

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Seven Common Allergen Groups Causing Eyelid Dermatitis: Education and Avoidance Strategies.

Clin Ophthalmol 2021 12;15:1477-1490. Epub 2021 Apr 12.

Department of Ophthalmology, Mayo Clinic, Scottsdale, AZ, USA.

Objective: Eyelid dermatitis is most commonly attributed to allergic response. This retrospective clinical study identifies common allergens with eyelid involvement and addresses a literary gap by providing a clear approach for effective management of periorbital allergic contact dermatitis (ACD) recurrence.

Methods: Charts of 215 patients diagnosed with periorbital dermatitis who were patch tested with Mayo Clinic Standard Series, Extended Standard Series, and personal products from 2013 to 2017 were examined. Read More

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Differential diagnosis of brain lesions in a metastatic endometrial carcinosarcoma patient.

Ecancermedicalscience 2021 9;15:1182. Epub 2021 Feb 9.

Division of Advanced Internal Medicine, Department of Medicine, National University Hospital, National University Health System, 1E Kent Ridge Road, Singapore 119228, Singapore.

The differential diagnosis of ring-enhancing brain lesions in a patient with metastatic malignancy may initially seem straightforward, and easily attributed to brain metastases. On rare occasions, the physician needs to avoid anchoring bias by re-evaluating the entire clinical context in which these ring-enhancing brain lesions are found. We report a case of cerebral toxoplasmosis mimicking brain metastases in a patient with metastatic cancer and without a prior history of human immunodeficiency virus. Read More

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February 2021

Charged aerosol detection in early and late-stage pharmaceutical development: selection of regressionmodels at optimum power function value.

J Chromatogr A 2021 Mar 12;1641:461997. Epub 2021 Feb 12.

Analytical Research & Development, MRL, Merck & Co., Inc., Rahway, NJ 07065, USA. Electronic address:

In recent years, the use of quantitative liquid chromatography (LC) coupled charged aerosol detection (CAD) for poor UV absorbing analytes in multicomponent mixtures has grown exponentially across academic and industrial sectors. The ballpark of previous LC-CAD reports is focused on practical applications, as well as optimization of critical parameters such as: response dependencies on temperature, nebulization process, analyte volatility, and mobile-phase composition. However, straightforward approaches to deal with the characteristic nonlinear response of CAD still scarce. Read More

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Septic gonococcal arthritis in a pediatric patient: Rare case report.

Int J Surg Case Rep 2021 Mar 24;80:105701. Epub 2021 Feb 24.

Community Memorial Health System, Ventura, CA, USA.

Introduction: Septic arthritis is an orthopedic emergency that requires rapid diagnosis and treatment due to the rapid destruction to cartilage. The responsible organism and etiology differs depending on patient age, especially in children. Gonococcal Arthritis in toddlers is a rare occurrence with few documented cases in the literature. Read More

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Green-tea-synthesized silver nanoparticles as a sensing platform for determination of tetracycline in honey samples.

J Sci Food Agric 2021 Feb 20. Epub 2021 Feb 20.

INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Bahía Blanca, Argentina.

Background: Silver nanoparticles (AgNPs) can be easily obtained in aqueous solution by chemical reduction using appropriate reducing agents and stabilizers. The development of environmentally friendly methods using non-toxic solvents and reagents has become an alternative for the synthesis of these particles and their future application as sensor probes for agricultural products. In this work, a straightforward method based on green tea extracts as reducing and capping agent is proposed for the synthesis of AgNPs, followed by their evaluation as a sensing platform for determination of tetracycline in honey samples. Read More

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February 2021

Consensus Report by the Italian Academy of Osseointegration on the Use of Antibiotics and Antiseptic Agents in Implant Surgery.

Int J Oral Maxillofac Implants 2021 Jan-Feb;36(1):103-105

Purpose: Beneficial aspects of short and ultrashort antibiotic administration protocols could be clinically correlated to the reduced side effects on the gastrointestinal microflora. The aim of this Consensus Conference was to establish the necessity of an antibiotic prophylaxis and its dosage to reduce the risk of early implant failure in healthy (ASA 1 or 2), periodontally healthy patients, undergoing basic dental implant surgery (straightforward cases). Additionally, the need for an antiseptic protocol, used before and after the implant surgery, was evaluated. Read More

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February 2021

Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana.

J Vis Exp 2021 01 16(167). Epub 2021 Jan 16.

Center for Immunotherapy, Vaccines and Virotherapy, The Biodesign Institute, Arizona State University; School of Life Sciences, Arizona State University; Molecular Biosciences/Biotechnology Undergraduate Program, Arizona State University;

High demand for antibodies as therapeutic interventions for various infectious, metabolic, autoimmune, neoplastic, and other diseases creates a growing need in developing efficient methods for recombinant antibody production. As of 2019, there were more than 70 FDA-approved monoclonal antibodies, and there is exponential growth potential. Despite their promise, limiting factors for widespread use are manufacturing costs and complexity. Read More

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January 2021

Pelargonium sidoides root extract for the treatment of acute cough due to lower respiratory tract infection in adults: a feasibility double-blind, placebo-controlled randomised trial.

BMC Complement Med Ther 2021 Jan 29;21(1):48. Epub 2021 Jan 29.

School of Primary Care, Population Sciences and Medical Education, University of Southampton, Aldermoor Health Centre, Aldermoor Close, Southampton, SO16 5ST, UK.

Background: Pelargonium sidoides DC (Geraniaceae) root extract, EPs®7630 or "Kaloba®", is a widely used herbal remedy for respiratory infections, with some evidence of effectiveness for acute bronchitis. However, it is not yet widely recommended by medical professionals in the UK. There is a need to undertake appropriately designed randomised trials to test its use as an alternative to antibiotics. Read More

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January 2021

Establish axenic cultures of armored and unarmored marine dinoflagellate species using density separation, antibacterial treatments and stepwise dilution selection.

Sci Rep 2021 Jan 8;11(1):202. Epub 2021 Jan 8.

Department of Science, School of Science and Technology, The Open University of Hong Kong, Ho Man Tin, Hong Kong.

Academic research on dinoflagellate, the primary causative agent of harmful algal blooms (HABs), is often hindered by the coexistence with bacteria in laboratory cultures. The development of axenic dinoflagellate cultures is challenging and no universally accepted method suit for different algal species. In this study, we demonstrated a promising approach combined density gradient centrifugation, antibiotic treatment, and serial dilution to generate axenic cultures of Karenia mikimotoi (KMHK). Read More

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January 2021

Mussel-Inspired Polymeric Coatings to Realize Functions from Single and Dual to Multiple Antimicrobial Mechanisms.

ACS Appl Mater Interfaces 2021 Jan 5;13(2):3089-3097. Epub 2021 Jan 5.

College of Materials Science & Engineering, Zhejiang University of Technology, Hangzhou 310014, P. R. China.

Numerous efforts to fabricate antimicrobial surfaces by simple yet universal protocols with high efficiency have attracted considerable interest but proved to be particularly challenging. Herein, we designed and fabricated a series of antimicrobial polymeric coatings with different functions from single to multiple mechanisms by selectively utilizing diethylene glycol diglycidyl ether (PEGDGE), polylysine, and poly[glycidylmethacrylate--3-(dimethyl(4-vinylbenzyl)ammonium)propyl sulfonate] (poly(GMA--DVBAPS)) via straightforward mussel-inspired codeposition techniques. Bactericidal polylysine endowed the modified surfaces with a high ability (∼90%) to kill attached bacteria, while PEGDGE components with unique surface hydration prevented bacterial adhesion, avoiding the initial biofilm formation. Read More

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January 2021

One-step electrochemically induced counterion exchange to construct free-standing carboxylated cellulose nanofiber/metal composite hydrogels.

Carbohydr Polym 2021 Feb 3;254:117464. Epub 2020 Dec 3.

School of Resource and Environmental Sciences, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Engineering Center of Natural Polymers-Based Medical Materials, Wuhan University, Wuhan 430079, China. Electronic address:

The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications. Herein, we report a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels. Metal ions were electrochemically and locally released from the electrode and chelated with carboxylated cellulose nanofibers, leading to the in-situ formation of composite hydrogels. Read More

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February 2021

Functionalization of eggshell membranes with CuO-ZnO based p-n junctions for visible light induced antibacterial activity against Escherichia coli.

Sci Rep 2020 12 1;10(1):20960. Epub 2020 Dec 1.

National Institute of Materials Physics, Atomistilor 405A, 077125, Magurele, Romania.

Biopolymers provide versatile platforms for designing naturally-derived wound care dressings through eco-friendly pathways. Eggshell membrane (ESM), a widely available, biocompatible biopolymer based structure features a unique 3D porous interwoven fibrous protein network. The ESM was functionalized with inorganic compounds (Ag, ZnO, CuO used either separately or combined) using a straightforward deposition technique namely radio frequency magnetron sputtering. Read More

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December 2020

Eosinopenia as predictor of infection in patients admitted to an internal medicine ward: a cross-sectional study.

Porto Biomed J 2020 Nov-Dec;5(6):e084. Epub 2020 Nov 11.

Assistente Hospitalar Graduada Sénior em Medicina Interna, Internal Medicine Department, Centro Hospitalar Universitário Cova da Beira, Covilhã, Portugal.

Background: The identification of infection in an internal medicine ward is crucial but not always straightforward. Eosinopenia has been proposed as a marker of infection, but specific cutoffs for prediction are not established yet. We aim to assess whether there is difference in eosinophil count between infected and noninfected patients and, if so, the best cutoffs to differentiate them. Read More

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November 2020

Generation of a lenalidomide-sensitive syngeneic murine in vivo multiple myeloma model by expression of Crbn.

Exp Hematol 2021 01 11;93:61-69.e4. Epub 2020 Nov 11.

Department of Internal Medicine III, Ulm University Hospital, Ulm, Germany; Department for Hematology, Oncology and Tumor Immunology, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany. Electronic address:

The immunomodulatory drugs (IMiDs) thalidomide, lenalidomide, and pomalidomide are approved drugs for the treatment of multiple myeloma. IMiDs induce cereblon (CRBN) E3 ubiquitin ligase-mediated ubiquitination and degradation of Ikaros transcription factors Ikaros (IKZF1) and Aiolos (IKZF3), which are essential for multiple myeloma. However, because of a single amino acid substitution of valine to isoleucine in mouse CRBN at position 391, mice are not susceptible to IMiD-induced degradation of neosubstrates. Read More

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January 2021

A Comprehensive Toolkit for Quick and Easy Visualization of Marker Proteins, Protein-Protein Interactions and Cell Morphology in .

Front Plant Sci 2020 15;11:569194. Epub 2020 Oct 15.

Institute for Plant Sciences, Faculty of Mathematics and Natural Sciences, University of Cologne, Cologne, Germany.

Even though stable genomic transformation of sporelings and thalli of is straightforward and efficient, numerous problems can arise during critical phases of the process such as efficient spore production, poor selection capacity of antibiotics or low transformation efficiency. It is therefore also desirable to establish quick methods not relying on stable transgenics to analyze the localization, interactions and functions of proteins of interest. The introduction of foreign DNA into living cells via biolistic mechanisms has been first reported roughly 30 years ago and has been commonly exploited in established plant model species such as or . Read More

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October 2020

Risk prediction models to guide antibiotic prescribing: a study on adult patients with uncomplicated upper respiratory tract infections in an emergency department.

Antimicrob Resist Infect Control 2020 11 2;9(1):171. Epub 2020 Nov 2.

Department of Clinical Epidemiology, Office of Clinical Epidemiology, Analytics and Knowledge, Tan Tock Seng Hospital, Singapore, Singapore.

Background: Appropriate antibiotic prescribing is key to combating antimicrobial resistance. Upper respiratory tract infections (URTIs) are common reasons for emergency department (ED) visits and antibiotic use. Differentiating between bacterial and viral infections is not straightforward. Read More

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November 2020

Oral Antibiotic Treatment Options for Acne Vulgaris.

Hilary Baldwin

J Clin Aesthet Dermatol 2020 Sep 1;13(9):26-32. Epub 2020 Sep 1.

Dr. Baldwin is the Medical Director of the Acne Treatment and Research Center in Brooklyn, New York and is with Rutgers Robert Wood Johnson Medical Center in New Brunswick, New Jersey.

Acne vulgaris is the most common dermatological disease in the United States, affecting up to 85 percent of teenagers. While the American Academy of Dermatology has established guidelines regarding acne treatment in general, the variability among acne treatments, even within a given class, prevents establishment of a straightforward regimen. For example, moderate to severe acne is generally treated with an oral antibiotic, although several options are available-both across and within antibiotic classes. Read More

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September 2020

Glutathione-Responsive Multifunctional "Trojan Horse" Nanogel as a Nanotheranostic for Combined Chemotherapy and Photodynamic Anticancer Therapy.

ACS Appl Mater Interfaces 2020 Nov 27;12(45):50896-50908. Epub 2020 Oct 27.

School of Materials and Energy & Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and Devices, Southwest University, Chongqing 400715, P. R. China.

It remains a great challenge to design a multifunctional and robust nanoplatform for stimuli-responsive drug delivery toward a lesion, which tactfully integrates multiple molecules with therapeutic and diagnostic characteristics. Herein, we reported a facile and ingenious cross-linked nanogel (DSA) based on the chemical cross-link of drugs as a straightforward strategy to overcome the instability of the assembly. In DSA, doxorubicin (DOX) and 5-aminolevulinic acid (ALA) were cross-linked with a disulfide linker for realizing synergistic anticancer therapy. Read More

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November 2020

PEGylation of Polyethylenimine Lowers Acute Toxicity while Retaining Anti-Biofilm and β-Lactam Potentiation Properties against Antibiotic-Resistant Pathogens.

ACS Omega 2020 Oct 29;5(40):26262-26270. Epub 2020 Sep 29.

Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.

Bacterial biofilms, often impenetrable to antibiotic medications, are a leading cause of poor wound healing. The prognosis is worse for wounds with biofilms of antimicrobial-resistant (AMR) bacteria, such as methicillin-resistant (MRSA), methicillin-resistant (MRSE), and multi-drug resistant (MDR-PA). Resistance hinders initial treatment of standard-of-care antibiotics. Read More

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October 2020

Surface Zwitterionization of Expanded Poly(tetrafluoroethylene) via Dopamine-Assisted Consecutive Immersion Coating.

ACS Appl Mater Interfaces 2020 Sep 1;12(37):41000-41010. Epub 2020 Sep 1.

R&D Center for Membrane Technology, Chung Yuan Christian University, Chungli, Taoyuan 32023, Taiwan.

Expanded polytetrafluoroethylene (ePTFE) is one of the materials widely used in the biomedical field, yet its application is being limited by adverse reactions such as thrombosis when it comes in contact with blood. Thus, a simple and robust way to modify ePTFE to be biologically inert is sought after. Modification of ePTFE without high-energy pretreatment, such as immersion coating, has been of interest to researchers for its straightforward process and ease in scaling up. Read More

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September 2020

Antibiotic dosage prescribed in oral implant surgery: A meta-analysis of cross-sectional surveys.

PLoS One 2020 18;15(8):e0236981. Epub 2020 Aug 18.

Department of Oral Health Sciences, Section Periodontology, Catholic University of Leuven & University Hospitals Leuven, Leuven, Belgium.

This study aimed to assess the dosage and types of antibiotics prescribed in oral implant surgery, compare them among the different subpopulations (country and prescription regimens) and against the evidence-based recommended dosage: a 2-gram single preoperative dose of amoxicillin. A meta-analysis of cross-sectional surveys was conducted, which reports the overall dosage (and type) of antibiotics prescribed in combination with implant placement. PubMed, Cochrane, Science, Direct, and EMBASE via OVID were searched until April 2019. Read More

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October 2020

Single-Cell Analysis of Signaling Proteins Provides Insights into Proapoptotic Properties of Anticancer Drugs.

Anal Chem 2020 09 27;92(18):12498-12508. Epub 2020 Aug 27.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Single-cell DNA analysis technology has provided unprecedented insights into many physiological and pathological processes. In contrast, technologies that allow protein analysis in single cells have lagged behind. Herein, a method called single-cell Plasmonic ImmunoSandwich Assay (scPISA) that is capable of measuring signaling proteins and protein complexes in single living cells is described. Read More

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September 2020

Synthesis of Degraded Limonoid Analogs as New Antibacterial Scaffolds against .

Antibiotics (Basel) 2020 Aug 6;9(8). Epub 2020 Aug 6.

Department of Organic Chemistry, Faculty of Sciences, Campus Universitario Río San Pedro s/n, Torre Sur, 4a; planta, University of Cádiz, 11510 Puerto Real, 11009 Cádiz, Spain.

and methicillin-resistant (MRSA) have become serious infections in humans and ruminants. strains are showing rapid changes to develop resistance in traditional antibiotic-containing systems. In the continuous fierce fight against the emergent multi-drug resistant bacterial strains, straightforward and scalable synthetic procedures to produce new active molecules are in demand. Read More

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Monitoring of Hydrogen Emission from Bacteria in Food, Animals and in the Blood of Humans Suffering from Lyme Disease by A Specific Hydrogen Sensor.

Antibiotics (Basel) 2020 Jul 21;9(7). Epub 2020 Jul 21.

Student Research Centre SFZ, D-88662 Überlingen, Germany.

A novel straightforward analytical technique was developed to monitor the emission of hydrogen from anaerobic bacteria cultured in sealed headspace vials using a specific hydrogen sensor. The results were compared with headspace gas chromatography carried out in parallel. This technique was also applied to investigate the efficacy of chemical antibiotics and of natural compounds with antimicrobial properties. Read More

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Knowledge and Prescription Habits toward Preoperative Antibiotics in Implant Dentistry: A Survey Analysis in a Subset of Dentists in Saudi Arabia.

J Contemp Dent Pract 2020 May 1;21(5):568-574. Epub 2020 May 1.

Periodontics Division, Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh, Kingdom of Saudi Arabia, Phone: +966 505556442, e-mail:

Aim: To investigate and evaluate current knowledge and habits in prescribing preoperative antibiotic, and toward specific practice situations, in a subset of dental practitioners performing routine dental implant surgery in Saudi Arabia.

Materials And Methods: A cross-sectional questionnaire-based study was developed, and relevant information was gathered through a web-based survey from a dentist who performed surgical implant placement. Participant demographics, clinical experience, and educational background were obtained. Read More

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Is perioperative antibiotic necessary in straightforward implant placement procedures?

J Oral Implantol 2020 Jul 14. Epub 2020 Jul 14.

Istanbul Aydin University, Department of Periodontology.

The purpose of this non-random retrospective cohort study was to evaluate the impact of prophylactic antibiotic on early outcomes including postoperative pain, swelling, bleeding and cyanosis in patients undergoing dental implant placement before prosthetic loading. Seventy-five patients (45 males, 30 females) whose dental implant placement were completed, included to the study. Patients used prophylactic antibiotics were defined as the experimental group and those who did not, were defined as the control group. Read More

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Lateral Gene Transfer Shapes Diversity of spp.

Front Cell Infect Microbiol 2020 23;10:293. Epub 2020 Jun 23.

Department of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, United States.

spp. are pathognomonic for bacterial vaginosis, which increases the risk of preterm birth and the transmission of sexually transmitted infections. spp. Read More

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Pharmacomodulations of the benzoyl-thiosemicarbazide scaffold reveal antimicrobial agents targeting d-alanyl-d-alanine ligase in bacterio.

Eur J Med Chem 2020 Aug 18;200:112444. Epub 2020 May 18.

Medicinal Chemistry Research Group (CMFA), Louvain Drug Research Institute (LDRI), Université catholique de Louvain (UCLouvain), 73 Avenue Mounier, B1.73.10, 1200, Bruxelles, Belgium. Electronic address:

d-Alanyl-d-alanine ligase (Ddl) is a validated and attractive target among the bacterial enzymes involved in peptidoglycan biosynthesis. In the present work, we investigated the pharmacomodulations of the benzoylthiosemicarbazide scaffold to identify new Ddl inhibitors with antibacterial potency. Five novel series of thiosemicarbazide analogues, 1,2,4-thiotriazole-3-thiones, 1,3,4-thiadiazoles, phenylthiosemicarbazones, diacylthiosemicarbazides and thioureas were synthesized via straightforward procedures, then tested against Ddl and on susceptible or resistant bacterial strains. Read More

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Sustainable Straightforward Synthesis and Evaluation of the Antioxidant and Antimicrobial Activity of Sinapine and Analogues.

J Agric Food Chem 2020 Jul 16;68(26):6998-7004. Epub 2020 Jun 16.

URD Agro-Biotechnologies Industrielles (ABI), CEBB, AgroParisTech, 51110, Pomacle, France.

Naturally occurring sinapine was successfully synthesized through a proline-mediated Knoevenagel-Doebner condensation in ethanol. This synthetic process involving biobased syringaldehyde, Meldrum's acid, and choline chloride offers a sustainable alternative to the existing low-yield pathways. This two-step strategy gives access to sinapine in a 52% overall yield and has been implemented in the synthesis of sinapine analogues, using 4-hydroxybenzaldehyde, 3,4-dihydroxybenzaldehyde, and vanillin as precursors, giving target molecules with 34-61% overall isolated yields. Read More

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