7 results match your criteria anomerism

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Anomeric Retention of Carbohydrates in Multistage Cyclic Ion Mobility (IMS): De Novo Structural Elucidation of Enzymatically Produced Mannosides.

Anal Chem 2021 04 8;93(15):6254-6261. Epub 2021 Apr 8.

INRAE, UR BIA, F-44316 Nantes, France.

Carbohydrates are complex structures that still challenge analysts today because of their different levels of isomerism, notably the anomerism of the glycosidic bond. It has been shown recently that anomerism is preserved upon gas-phase fragmentation and that high-resolution ion mobility (IMS) can distinguish anomers. However, these concepts have yet to be applied to complex biological products. Read More

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Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.

Anal Chem 2020 10 11;92(19):13211-13220. Epub 2020 Sep 11.

Center for Biomedical Mass Spectrometry, Boston University School of Medicine, 670 Albany Street, Room 508, Boston, Massachusetts 02118, United States.

Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment. However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers. Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS). Read More

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October 2020

Effect of the Anomeric Configuration on the Micellization of Hexadecylmaltoside Surfactants.

Langmuir 2019 Oct 14;35(43):13904-13914. Epub 2019 Oct 14.

Enza Biotech AB , Scheelevägen 22 , 22363 Lund , Sweden.

The self-assembly of the two anomeric forms of -hexadecyl-d-maltopyranoside (denoted α-CG and β-CG) has been studied in dilute aqueous solution by means of surface tension measurements, scattering methods (dynamic light scattering, static light scattering, and small-angle X-ray and neutron scattering), and cryo-transmission electron microscopy at different surfactant concentrations and temperatures. Surface tension measurements demonstrate differences in the surfactant adsorption at the air-water interface, where α-CG shows a lower CMC than β-CG. Similarly, micelle morphology was found to profoundly depend on anomerism. Read More

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October 2019

Membrane property and biofunction of phospholiposome incorporated with anomeric galactolipids.

Springerplus 2016 17;5:655. Epub 2016 May 17.

Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai, 200237 People's Republic of China.

There has been increasing interest in the construction of liposomes containing a targeting reagent for target-specific drug delivery. Glycoconjugates that can be recognized by transmembrane glycoprotein receptors have been extensively used to form glyco-liposomal drug carriers. However, the impact of anomerism, which is a common identity of natural glycoconjugates, on the glyco-liposomal properties has been hardly probed in previous studies. Read More

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Nanoscale reversed-phase liquid chromatography-mass spectrometry of permethylated N-glycans.

Anal Bioanal Chem 2013 Mar 11;405(8):2469-80. Epub 2013 Jan 11.

Finnish Red Cross Blood Service, Helsinki, Finland.

Reversed-phase liquid chromatography on the nanoscale coupled to electrospray tandem mass spectrometry was used to analyse a mixture of four commercial glycan standards, and the method was further adapted to N-glycans enzymatically released from alpha-1-acid glycoprotein and immunoglobulin gamma. Glycans were permethylated to enable their separation by reversed-phase chromatography and to facilitate interpretation of fragmentation data. Prior to derivatization of glycans by permethylation, they were reduced to cancel anomerism because, although feasible, it was not desired to separate α- and β-anomers. Read More

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Unambiguous structural elucidation of base-modified purine nucleosides using NMR.

Magn Reson Chem 2008 Jan;46(1):1-8

Department of Chemistry, Open University, Walton Hall, Milton Keynes MK7 6AA, UK.

A general and unambiguous approach has been developed for structural elucidation of modified purine nucleosides using NMR spectroscopy. Systematic assignment of proton and carbon signals of modified nucleosides was firmly established by COSY and the anomerism of the glycosidic linkage of synthetic nucleosides clearly elucidated by NOESY experiments. Characteristic properties of 15N-isotopic labelling at specific positions of nucleosides were also employed for structural studies. Read More

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January 2008

Triterpenoid carotenoids and related lipids. Triterpenoid monohydroxy- and monoglucosyloxy-carotenoids from Streptococcus faecium UNH 564P.

Biochem J 1974 Jun;139(3):761-9

1. The characterization of two novel triterpenoid xanthophylls occurring in Streptococcus faecium UNH 564P is described. 2. Read More

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