12,174 results match your criteria anion-exchange chromatography

Purification and Characterization of a New Halocin HA4 from Haloferax larsenii HA4 Isolated from a Salt Lake.

Probiotics Antimicrob Proteins 2021 Jul 20. Epub 2021 Jul 20.

Department of Genetics, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.

Halocins are antimicrobial peptides secreted by different members of haloarchaea. Halocin HA4 was purified from Haloferax larsenii HA4 using combination of ultrafiltration and chromatographic techniques. It was found to be ~ 14 kDa with unique N-terminal sequence, HN-AEEEIFXPDX, which did not show homology with the known sequence suggesting a new/novel compound. Read More

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Candidate reference measurement procedure based on HPAEC-PAD for the development of certified reference materials for monosaccharides in serum.

Anal Bioanal Chem 2021 Jul 20. Epub 2021 Jul 20.

Biometrology Group, Division of Chemical and Biological Metrology, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-Gu, Daejeon, 34113, Republic of Korea.

To achieve the measurement reliability of monosaccharides used as diagnostic markers in clinical fields, it is essential to establish certified reference materials (CRMs). The purpose of this study is to develop a serum CRM by adopting high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) as a new candidate reference measurement procedure for the measurement of glucose and galactose, common diagnostic markers of diabetes and galactosemia, respectively. Using various monosaccharides as internal standards, the accuracy of the HPAEC-PAD method was tested by measuring glucose CRM following treatment with three different deproteinization methods: ultrafiltration, protein precipitation by trichloroacetic acid (TCA), and protein precipitation by acetonitrile. Read More

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Simultaneous quantitation of inorganic ions in oligonucleotides using mixed-mode liquid chromatography coupled with a charged aerosol detector.

J Pharm Biomed Anal 2021 Jul 8;204:114244. Epub 2021 Jul 8.

Department of Analytical Development, Biogen, 225 Binney St, Cambridge, MA 02142, USA.

The amounts of inorganic ions (e.g. sodium, potassium, magnesium, calcium, chloride, and phosphate) in intrathecally administered oligonucleotide drug products need to be controlled in order to meet the final target formulation composition. Read More

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Evaluation of guanidine-based multimodal anion exchangers for protein selectivity and orthogonality.

J Chromatogr A 2021 Jul 9;1653:462398. Epub 2021 Jul 9.

Department of Chemical and Biological Engineering and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, United States. Electronic address:

In this paper, we examined the chromatographic behavior of a new class of guanidine-based multimodal anion exchange resins. The selectivities and protein recoveries on these resins were first evaluated using linear gradient chromatography with a model acidic protein library at pH 5, 6 and 7. While a single-guanidine based resin exhibited significant recovery issues at high ligand density, a bis-guanidine based resin showed high recoveries of all but two of the proteins evaluated in the study. Read More

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Biorefining Potential of Wild-Grown , and and the Feasibility of White-Rot Fungi-Mediated Pretreatments.

Front Plant Sci 2021 2;12:679966. Epub 2021 Jul 2.

Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Coimbra, Portugal.

, and are high-biomass-producing perennial Poalean species that grow abundantly and spontaneously in warm temperate regions, such as in Mediterranean-type climates, like those of Southern Europe, Western United States coastal areas, or in regions of South America, South Africa and Australia. Given their vigorous and spontaneous growth, biomass from the studied grasses often accumulates excessively in unmanaged agro-forestry areas. Nonetheless, this also creates the demand and opportunity for the valorisation of these biomass sources, particularly their cell wall polymers, for biorefining applications. Read More

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Expression, purification and X-ray crystal diffraction analysis of alcohol dehydrogenase 1 from Artemisia annua L.

Protein Expr Purif 2021 Jul 14;187:105943. Epub 2021 Jul 14.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China. Electronic address:

Alcohol dehydrogenase 1 identified from Artemisia annua (AaADH1) is a 40 kDa protein that predominately expressed in young leaves and buds, and catalyzes dehydrogenation of artemisinic alcohol to artemisinic aldehyde in artemisinin biosynthetic pathway. In this study, AaADH1 encoding gene was subcloned into vector pET-21a(+) and expressed in Escherichia coli. BL21(DE3), and purified by Co affinity chromatography. Read More

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A fit-for-purpose copper speciation method for the determination of exchangeable copper relevant to Wilson's disease.

Anal Bioanal Chem 2021 Jul 16. Epub 2021 Jul 16.

LGC Limited, National Measurement Laboratory (NML), Queens Road, Teddington, Middlesex, TW11 0LY, UK.

Exchangeable copper (CuEXC), mainly comprised copper (Cu) bound to albumin, has been proposed as a specific marker of Cu overload in Wilson's disease (WD). To the author's knowledge, there are no methods capable of determining reliably CuEXC to meet the requirements and challenges faced by a clinical trial. The present work describes a novel speciation strategy for the determination of the main Cu-species in human serum by anion-exchange high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Read More

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Surface-confined guanidinium ionic liquid as a new type of stationary phase for hydrophilic interaction liquid chromatography.

J Sep Sci 2021 Jul 16. Epub 2021 Jul 16.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116023, China.

Guanidinium-based ionic liquids possess lower toxicity and greater designability than commonly used species and have presented good performances in liquid-phase extraction and stationary phase for capillary GC. In the present work, a novel type of surface-confined guanidinium ionic liquid stationary phase was developed by bonding a hexaalkylguanidinium ionic liquid N,N,N',N'-tetramethyl-N",N"-diallylguanidinium bromide onto the surface of 3-mercaptopropyl modified silica. The obtained surface-confined guanidinium ionic liquid silica materials were characterized by elemental analysis, infrared spectrum, and thermogravimetric analysis, and then packed as a HPLC column for the evaluation of chromatographic retention behavior. Read More

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Cationic Covalent Organic Framework as an Ion Exchange Material for Efficient Adsorptive Separation of Biomolecules.

ACS Appl Mater Interfaces 2021 Jul 15. Epub 2021 Jul 15.

Ralph E. Martin Department of Chemical Engineering, University of Arkansas, Fayetteville, Arkansas 72701, United States.

Although covalent organic frameworks (COFs) have earned significant interest in separation applications, the use of COFs in biomolecule separation remains unexplored. We examined the ionic COF Py-BPy-COF as an ion exchange material for biomolecule separation. After characterizing the properties of the synthesized COF with a variety of techniques, binding experiments with both large and small biomolecules were performed. Read More

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Precision analysis for the determination of steric mass action parameters using eight tobacco host cell proteins.

J Chromatogr A 2021 Jun 30;1652:462379. Epub 2021 Jun 30.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, Aachen 52074, Germany; Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, Aachen 52074, Germany. Electronic address:

Plants are advantageous as biopharmaceutical manufacturing platforms because they allow the economical and scalable upstream production of proteins, including those requiring post-translational modifications, but do not support the replication of human viruses. However, downstream processing can be more labor-intensive compared to fermenter-based systems because the product is often mixed with abundant host cell proteins (HCPs). Modeling chromatographic separation can minimize the number of process development experiments and thus reduce costs. Read More

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Fast and high-resolution purification of a PEGylated protein using a z laterally-fed membrane chromatography device.

J Chromatogr A 2021 Jun 29;1652:462375. Epub 2021 Jun 29.

Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4L7, Canada. Electronic address:

PEGylated proteins comprise a class of value-added biopharmaceuticals. High-resolution separation techniques are required for the purification of these molecules. In this study, we discuss the application of a newly developed z laterally-fed membrane chromatography (or zLFMC) device for carrying out high-resolution purification of a PEGylated protein drug. Read More

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Glycoconjugation of Shigella flexneri type 2a O-polysaccharide with CRM as a potential vaccine candidate for shigellosis.

Biologicals 2021 Jul 8. Epub 2021 Jul 8.

Department of Biological Sciences, BITS Pilani Hyderabad Campus, Jawahar Nagar, Hyderabad Campus, Kapra Mandal, Medchal, Telangana, 500078, India. Electronic address:

Shigellosis, a diarrheal disorder caused by an entero-invasive bacterium Shigella, is a major concern among children often leading to mortality. As most of these strains have developed universal antibiotic resistance, the development of a vaccine is crucial in combating the infection. The O-specific polysaccharide (O-PSs) from S. Read More

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Structural elucidation and physicochemical characteristics of a novel high-molecular-weight fructan from halotolerant Bacillus sp. SCU-E108.

Food Chem 2021 Jun 30;365:130496. Epub 2021 Jun 30.

College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, PR China; Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu 610065, PR China. Electronic address:

An exopolysaccharide, EPS-B108, was isolated from the fermented broth (with a yield of 11.3 g/L) of halotolerant Bacillus sp. SCU-E108 by ethanol precipitation, anion-exchange and gel-filtration chromatography, and well characterized by means of physical, chemical and spectral techniques. Read More

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The Determination of Carbohydrates by High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection (HPAEC-PAD).

Methods Mol Biol 2021 ;2356:57-68

Johannes Gutenberg University Mainz, Microbiology and Wine Research at the Institute for Molecular Physiology, Mainz, Germany.

Chromatography techniques are widely used to separate, identify, and quantify molecules depending on their physicochemical properties. Standard methods range from simple size exclusion to separation based on affinity or ion exchange. Here, we present a method for the direct analysis of carbohydrates in Magnaporthe oryzae using high-performance anion-exchange chromatography (HPAEC) coupled with pulsed amperometric detection (PAD). Read More

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January 2021

Perception on aggregation induced multicolor emission and emission centers in carbon nanodots using successive dilution, anion exchange chromatography, and multi-way statistics.

Sci Rep 2021 Jul 7;11(1):13996. Epub 2021 Jul 7.

Department of Chemistry, Institute for Advanced Studies in Basic Sciences, 45137-66731, Zanjan, Iran.

Exploration in the way of understanding the optical behavior and structure of carbon nanodots has been increased due to their vast application. Their emission dependency on excitation wavelengths is the more prevalent and controversial subject. In this report we considered the optical structure of hydrothermally synthesized carbon nanodots using citric acid and 2,3-diaminopyridine as precursors. Read More

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[Preparation and chromatographic properties of 1-vinyl-3-dodecylimidazole bromide silica-bonded stationary phase].

Se Pu 2020 Nov;38(11):1263-1269

College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.

As the main components of cell membranes, lipids play important roles in organisms. Lipids have been proved to be closely associated with the occurrence and development of serious diseases, such as cancers and metabolic diseases. The development of novel separation materials for use in high-performance liquid chromatography (HPLC) is essential for high-efficiency lipid separation. Read More

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November 2020

Extraction and Characterization of Hemicelluloses from a Softwood Acid Sulfite Pulp.

Polymers (Basel) 2021 Jun 22;13(13). Epub 2021 Jun 22.

CNRS, University Bordeaux, Bordeaux INP, LCPO, UMR 5629, 33600 Pessac, France.

Hemicelluloses were extracted from a softwood acid sulfite pulp in a three-step procedure. Further delignification step resulted in a holocellulose pulp containing only 1.7 wt. Read More

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Structural Characterization of Pectic Polysaccharides in the Cell Wall of Stevens Variety Cranberry Using Highly Specific Pectin-Hydrolyzing Enzymes.

Polymers (Basel) 2021 Jun 2;13(11). Epub 2021 Jun 2.

Department of Food Science and Agricultural Chemistry, Macdonald Campus, McGill University, S.te-Anne-de-Bellevue, QC H9X 3V9, Canada.

The potential of poly- and oligosaccharides as functional ingredients depends on the type and glycosidic linkages of their monosaccharide residues, which determine their techno-functional properties, their digestibility and their fermentability. To isolate the pectic polysaccharides of cranberry, alcohol insoluble solids were first obtained from pomace. A sequential extraction with hot phosphate buffer, chelating agents (CH), diluted (DA) and concentrated sodium hydroxide was then carried out. Read More

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Crystal structure of a homotrimeric verrucomicrobial --1,4-mannosidase active in the hindgut of the wood-feeding termite .

J Struct Biol X 2021 24;5:100048. Epub 2021 May 24.

School of Engineering Sciences in Chemistry, Biotechnology, and Health (CBH), KTH Royal Institute of Technology, Roslagstullsbacken 21, SE-11421 Stockholm, Sweden.

The termite causes extensive damage due to the high efficiency and broad specificity of the ligno- and hemicellulolytic enzyme systems produced by its symbionts. Thus, the gut microbiome is expected to constitute an excellent source of enzymes that can be used for the degradation and valorization of plant biomass. The symbiont bacterium strain TAV5 belongs to the phylum Verrucomicrobia and thrives in the hindgut of . Read More

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Hydrophobicity of polymer based anion-exchange columns for ion chromatography.

Heliyon 2021 Jun 14;7(6):e07290. Epub 2021 Jun 14.

Department of Chemistry, Lomonosov Moscow State University, 1-3 Leninskie Gory, 119991 Moscow, Russian Federation.

The regularities of the retention of alkanoic and alkanesulfonic acids homologues were investigated for the set of 36 anion-exchange columns produced by various manufacturers. The role of hydrophobic and electrostatic interactions in the retention and separation of organic anions was studied. The methylene selectivity increments α(CH) were measured for the studied columns with 10 mM sodium hydroxide eluent. Read More

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Ion exchange solid phase microextraction coupled to liquid chromatography/laminar flow tandem mass spectrometry for the determination of perfluoroalkyl substances in water samples.

J Chromatogr A 2021 Aug 9;1651:462335. Epub 2021 Jun 9.

Department of Chemistry and Biochemistry, The University of Toledo, Toledo, OH, 436062, United States; Dr. Nina McClelland Laboratories for Water Chemistry and Environmental Analysis, The University of Toledo, Toledo, OH, 43606, United States; School of Green Chemistry and Engineering, The University of Toledo, Toledo, OH, 43606. Electronic address:

Per- and polyfluoroalkyl substances (PFAS) are toxic and bioaccumulative compounds that are persistent in the environment due to their water and heat resistant properties. These compounds have been demonstrated to be ubiquitous in the environment, being found in water, soil, air and various biological matrices. The determination of PFAS at ultra-trace levels is thus critical to assess the extent of contamination in a particular matrix. Read More

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Distribution, accumulation and speciation of selenium at the different growth stages of four garlic clones.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 Jun 22:1-14. Epub 2021 Jun 22.

Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina.

Selenium (Se) is an essential micronutrient for humans. Garlic ( L.) metabolises Se into important Se-amino acids like Se-methylselenocysteine (Se-MetSeCys), precursor of methylselenol, an active species for cancer prevention. Read More

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A one-year study of human milk oligosaccharide profiles in the milk of healthy UK mothers and their relationship to maternal FUT2 genotype.

Glycobiology 2021 Jun 15. Epub 2021 Jun 15.

Department of Food Science and Technology, University of California, Davis, USA.

Human milk oligosaccharides (HMOs) are indigestible carbohydrates with prebiotic, pathogen decoy, and immunomodulatory activities that are theorized to substantially impact infant health. The objective of this study was to monitor HMO concentrations over one year to develop a long-term longitudinal dataset. HMO concentrations in the breast milk of healthy lactating mothers of the Cambridge Baby Growth and Breastfeeding Study (CBGS-BF) were measured at birth, 2 weeks, 6 weeks, 3 months, 6 months and 12 months postpartum. Read More

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Efficient co-production of propionic acid and succinic acid by using membrane separation coupled technology.

Eng Life Sci 2021 Jun 7;21(6):429-437. Epub 2021 Jun 7.

State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. China.

To improve the fermentation efficiency of , a semi-continuous coupled fermentation process was established to achieve co-production of propionic acid (PA) and succinic acid (SA). First, the optimal proportion of glucose (Glc) and glycerol (Gl) as a mixed carbon source was determined, and the feeding procedure of Gl was optimized to make more energy flow in the direction of product synthesis. Then, ZGD630 anion exchange resin was used for efficient adsorption of PA, thereby eliminating the feedback inhibition effect of PA. Read More

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Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola.

Enzyme Microb Technol 2021 Aug 20;148:109801. Epub 2021 Apr 20.

Biocatalysis and Biosensing Laboratory, Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria. Electronic address:

Laccase is predominantly found in lignin degrading filamentous white rot fungi, where it is involved in the oxidative degradation of this recalcitrant heteropolymer. In brown rot fungi it is much less prevalent: laccases from only a few brown rots have been detected and only two have been characterized. This study tries to understand the role of this ligninolytic enzyme in brown rots by investigating the catalytic properties of laccases secreted by Fomitopsis pinicola FP58527 SS1. Read More

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High stability amino-derived reversed-phase/anion-exchange mixed-mode phase based on polysilsesquioxane microspheres for simultaneous separation of compound drugs.

J Pharm Biomed Anal 2021 Sep 3;203:114187. Epub 2021 Jun 3.

School of Pharmaceutical Science & Technology, Tianjin University, 92 Weijin Road, Tianjin, 300072, China. Electronic address:

A series of amino-derived mixed-mode chromatographic stationary phases were synthesized based on porous mercaptopropyl-functionalized polysilsesquioxane mesoporous microspheres synthesized by a co-condensation of methyltrimethoxysilane (MTMS) and mercaptopropyltrimethoxysilane (MPTMS). Through controlling the ratio of MTMS and MPTMS, the modified stationary phases with different amino densities were prepared by a "thiol-ene" click chemistry reaction. The morphology, pore structure, and functional groups of the microspheres were characterized by scanning electron microscope (SEM), nitrogen adsorption-desorption test, Fourier transform infrared spectroscopy (FT-IR), elemental analysis, and zeta potential, respectively. Read More

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September 2021

Determination of selenite and selenomethionine in kefir grains by reversed-phase high-performance liquid chromatography-inductively coupled plasma-optical emission spectrometry.

J Sep Sci 2021 Jun 8. Epub 2021 Jun 8.

Department of Chemistry, Faculty of Art and Science, Yıldız Technical University, Esenler, İstanbul, Turkey.

A new and efficient reversed-phase high-performance liquid chromatography-inductively coupled plasma-optical emission spectrometry method was developed for the simultaneous separation and determination of SeO and seleno-dl-methionine in kefir grains. For the system, limits of detection and quantitation values for SeO and seleno-dl-methionine were calculated as 0.52/1. Read More

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Correlation between protein desorption behavior and its adsorption enthalpy change in polymer grafted anion exchange chromatography.

Colloids Surf B Biointerfaces 2021 Sep 18;205:111853. Epub 2021 May 18.

Bio-Process Engineering Laboratory, Biomedical Engineering Center, Yamaguchi University, Tokiwadai 2-16-1, Ube 755-8611, Japan.

Thermodynamic studies on protein adsorption onto chromatographic surfaces mainly focus on the molecular level interaction between proteins and ligands. Yet, not much attention is given to the study of polymer grafted ligand architecture effect on thermodynamic parameters, nor to the relation between chromatographic parameters and the directly obtained thermodynamic parameters. These relations are needed in order to confer meaning and to ease future data interpretation of thermodynamic studies of protein adsorption. Read More

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September 2021

Sustainable valorization of meat processing wastewater with synergetic eutectic mixture based purification of R-Phycoerythrin from porphyrium cruentium.

Bioresour Technol 2021 Sep 2;336:125357. Epub 2021 Jun 2.

Green Separation Engineering Laboratory, SASTRA Deemed to be University, Thanjavur, Tamil Nadu 613401, India. Electronic address:

Wastewater effluent from meat processing industries are enriched with nutrients but remain underutilized. Therefore, an efficient method was administered by employing this resource for the cultivation of Porphyreum cruentium. The cultured biomass was exposed to a one-step disruption and selective purification method using a protic deep eutectic solvent to obtain a commercially significant pigment, R-Phycoerythrin (R-PE). Read More

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September 2021

Purification and characterization of a novel acid-tolerant and heterodimeric β-glucosidase from pumpkin (Cucurbita moschata) seed.

J Biosci Bioeng 2021 May 30. Epub 2021 May 30.

Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea; Center for Agricultural Microorganism and Enzyme, Seoul National University, Seoul 08826, Republic of Korea. Electronic address:

A novel β-glucosidase was purified from pumpkin (Cucurbita moschata) seed by anion exchange chromatography and gel permeation chromatography, and its molecular mass was determined to be 42.8 kDa by gel permeation chromatography. The heterodimeric structure consisting of two subunits, free from disulfide bonds, was determined by native-PAGE analysis followed by zymography. Read More

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