2,463,073 results match your criteria animal human

Sec14 family of lipid transfer proteins in yeasts.

Biochim Biophys Acta Mol Cell Biol Lipids 2021 Jun 9:158990. Epub 2021 Jun 9.

Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Bratislava, Slovakia. Electronic address:

The hydrophobicity of lipids prevents their free movement across the cytoplasm. To achieve highly heterogeneous and precisely regulated lipid distribution in different cellular membranes, lipids are transported by lipid transfer proteins (LTPs) in addition to their transport by vesicles. Sec14 family is one of the most extensively studied groups of LTPs. Read More

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Development of a novel model of intervertebral disc degeneration by the intradiscal application of monosodium iodoacetate (MIA) in rat Degenerative change in monosodium iodoacetate (MIA)-injected intervertebral disc.

Spine J 2021 Jun 9. Epub 2021 Jun 9.

Department of Physiology, Korea University, Seoul, Republic of Korea. Electronic address:

Background Context: Low back pain is one of the most common musculoskeletal disorders. While the pathology of intervertebral disc (IVD) degeneration has been modeled using various biological methods, these models are inadequate for simulating similar pathological states in humans.

Purpose: This study investigated whether monosodium iodoacetate (MIA) injection into the IVD of rats could generate a reliable model of IVD degeneration. Read More

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Identification and profiles of microRNAs in different development stages of miniature pig secondary palate.

Genomics 2021 Jun 9. Epub 2021 Jun 9.

Department of geriatric dentistry, Capital Medical University School of Stomatology, Tiantan Xili No.4, Beijing 100050, China; Laboratory of Molecular Signaling and Stem Cells Therapy, Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tiantan Xili No.4, Beijing 100050, China. Electronic address:

Cleft palate is one of the most frequent craniofacial malformation birth defects. Miniature pigs (Sus scrofa) are a valuable alternative large animal model to explore human palate development. Presently, the microRNA (miRNA) expression profiles in miniature pigs during palatogenesis from embryonic day (E) 30 to 50 were identified. Read More

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Zoonotic and Reverse Zoonotic Transmissibility of SARS-CoV-2.

Virus Res 2021 Jun 9:198473. Epub 2021 Jun 9.

National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine", 83 Pushkinska street, Kharkiv, 61023, Ukraine. Electronic address:

The Coronavirus Disease 2019 (COVID-19) is the first known pandemic caused by a coronavirus. Its causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), appears to be capable of infecting different mammalian species. Recent detections of this virus in pet, zoo, wild, and farm animals have compelled inquiry regarding the zoonotic (animal-to-human) and reverse zoonotic (human-to-animal) transmissibility of SARS-CoV-2 with the potential of COVID-19 pandemic evolving into a panzootic. Read More

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Food Chem Toxicol 2021 Jun 9:112334. Epub 2021 Jun 9.

Department of Veterinary Medicine and Animal Production, University of Naples, Via F. Delpino, 1, 80137, Napoli, Italy. Electronic address:

The occurrence of analogues of bisphenol A (BPA), including bisphenol F(BPF) in milk is still not well known. BPF may enter the milk chain at the farm and during milk processing at the dairy company. This study identified the main BPF contamination pathways using a monitoring model based on the identification of the hazard at three stages along the dairy chain: raw milk from the storage tank, pasteurized milk from the storage tank, and cardboard packaged milk. Read More

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Safety and efficacy of an anti-claudin-5 monoclonal antibody to increase blood-brain barrier permeability for drug delivery to the brain in a non-human primate.

J Control Release 2021 Jun 9. Epub 2021 Jun 9.

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan. Electronic address:

Claudin-5 (CLDN-5) is an essential component of the tight junction seal in the blood-brain barrier. Previously, we showed that CLDN-5 modulation in vitro via an anti-CLDN-5 monoclonal antibody (mAb) may be useful for increasing the permeability of the blood-brain barrier for drug delivery to the brain. Based on these findings, here we examined the safety and efficacy of the anti-CLDN-5 mAb in a non-human primate. Read More

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The squirrel monkey model in clinical neuroscience.

Neurosci Biobehav Rev 2021 Jun 9. Epub 2021 Jun 9.

Brain Connectivity and Behaviour Laboratory, Sorbonne Universities, Paris, France; Groupe d'Imagerie Neurofonctionnelle, Institut des Maladies Neurodégénératives-UMR 5293, CNRS, CEA University of Bordeaux, Bordeaux, France. Electronic address:

Clinical neuroscience research relying on animal models brought valuable translational insights into the function and pathologies of the human brain. The anatomical, physiological, and behavioural similarities between humans and mammals have prompted researchers to study cerebral mechanisms at different levels to develop and test new treatments. The vast majority of biomedical research uses rodent models, which are easily manipulable and have a broadly resembling organisation to the human nervous system but cannot satisfactorily mimic some disorders. Read More

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Live-Cell Assessment of Reactive Oxygen Species Levels Using Dihydroethidine.

Methods Mol Biol 2021 ;2275:291-299

Human and Animal Physiology, Wageningen University & Research, Wageningen, Netherlands.

Reactive oxygen species (ROS) play an important role in cellular (patho)physiology. Empirical evidence suggests that mitochondria are an important source of ROS, especially under pathological conditions. Here, we describe a method for ROS measurement using dihydroethidium (HEt) and live-cell microscopy. Read More

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January 2021

Blood-brain barrier leakage and perivascular collagen accumulation precede microvessel rarefaction and memory impairment in a chronic hypertension animal model.

Metab Brain Dis 2021 Jun 12. Epub 2021 Jun 12.

Koç University Research Center for Translational Medicine, Istanbul, Turkey.

Hypertension (HT) is one of the main causes of vascular dementia, lead to cognitive decline. Here, we investigated the relationship between cerebral microvessels, pericytes, extracellular matrix (ECM) accumulation, blood-brain barrier (BBB) breakdown, and memory impairment at mid-life in a chronic hypertension animal model. Spontaneously hypertensive rats (SHRs) (n = 20) are chosen for the model and age matched Wistar rats (n = 16) as controls. Read More

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AIM2-driven inflammasome activation in heart failure.

Cardiovasc Res 2021 Jun 12. Epub 2021 Jun 12.

Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.

Aims: Interleukin-1β (IL-1β) is an important pathogenic factor in cardiovascular diseases including chronic heart failure (HF). The CANTOS trial highlighted that inflammasomes as primary sources of IL-1 β are promising new therapeutic targets in cardiovascular diseases. Therefore, we aimed to assess inflammasome activation in failing hearts to identify activation patterns of inflammasome subtypes as sources of IL-1β. Read More

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NPT520-34 improves neuropathology and motor deficits in a transgenic mouse model of Parkinson's disease.

Brain 2021 Jun 12. Epub 2021 Jun 12.

Neuropore Therapies, Inc., 10835 Road to the Cure, Suite 230, San Diego, CA 92121 USA.

NPT520-34 is a clinical-stage, small molecule being developed for the treatment of Parkinson's disease and other neurodegenerative disorders. The therapeutic potential of NPT520-34 was first suggested by findings from cell-based assays of alpha-synuclein (ASYN) clearance. As reported here, NPT520-34 was subsequently evaluated for therapeutically relevant actions in a transgenic animal model of Parkinson's disease that overexpresses human ASYN and in an acute lipopolysaccharide (LPS)-challenge model using wild-type mice. Read More

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Meglumine Antimoniate-Loaded Aqueous-Core PLA Nanocapsules: Old Drug, New Formulation against Leishmania-Related Diseases.

Macromol Biosci 2021 Jun 12:e2100046. Epub 2021 Jun 12.

Department of Experimental and Clinical Medicine, University "Magna Graecia" of Catanzaro, Campus Universitario "S. Venuta,", Catanzaro, I-88100, Italy.

Leishmaniasis is a human and animal disease endemic in tropical and subtropical areas treated by means of pentavalent antimony as first-line approach. Unfortunately, the formulations available on the market are characterized by significant side effects and a total remission of the disease is difficult to be obtained. The aim of this investigation is to describe the development and characterization of aqueous-core poly-l-lactide (PLA) nanocapsules containing glucantime (meglumine antimoniate, MA) with the aim of increasing the pharmacological efficacy of the active compound. Read More

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Pancreatic enzyme activity in the bile of healthy cats and its association with biliary morphology.

J Vet Intern Med 2021 Jun 12. Epub 2021 Jun 12.

Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, 18-9 Kita, Kita Ward, Sapporo, Hokkaido, Japan.

Background: In human medicine, congenital maljunction of the common bile duct (CBD) and main pancreatic duct (MPD), or pancreatobiliary maljunction (PBM), is a known cause of cholecystitis.

Objective: Pancreatic enzyme activity in the bile (a diagnostic marker for PBM) of healthy cats was measured to determine normal values and evaluate its relationship with biliary morphology.

Animals: Fifty-two healthy cats. Read More

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Nicotinic acid supplementation at a supraphysiological dose increases the bioavailability of NAD precursors in mares.

J Anim Physiol Anim Nutr (Berl) 2021 Jun 12. Epub 2021 Jun 12.

Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia.

NAD deficiency has recently been linked with increased occurrences of congenital abnormalities and embryonic death in human and animal subjects. Early embryonic death is a major component of pregnancy loss in mares and very little is known regarding the requirement for NAD in horses. The aim of this study was to quantify NAD and its metabolites in the plasma and urine of mares after orally administering an acute dose of nicotinic acid and determine the absorption, metabolism and excretion of this essential precursor for NAD biosynthesis. Read More

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Animal models of chronic and recurrent Pseudomonas aeruginosa lung infection - significance of macrolide treatment.

APMIS 2021 Jun 12. Epub 2021 Jun 12.

Department of Clinical Microbiology, Rigshospitalet, Copenhagen University Hospital. Copenhagen, Copenhagen, Denmark.

Animal models of human diseases are invaluable and inevitable elements in identifying and testing novel treatments of serious diseases, including severe infections. Planning and conducting investigator initiated human trials are generally accepted as being enormously challenging. In contrast, it is often underestimated how much planning, including background and modifying experiments, there is needed to establish a relevant infectious disease animal model. Read More

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The Effect of Gamma and Microwave Radiation Sterilization on Periodontological Grafts for Microbiological Evaluation.

APMIS 2021 Jun 12. Epub 2021 Jun 12.

Hacettepe University, Faculty of Dentistry, Department of Periodontology, 06100, Sıhhiye Ankara, Turkey.

Purpose: Periodontological grafts are materials used in dentistry to regenerate lost gingival soft tissues or bone parts. In the case of direct contact with blood, the possibility of disease transmission from the source to the patient is high. This source can be an animal or a human. Read More

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Zebrafish disease models in drug discovery: from preclinical modelling to clinical trials.

Nat Rev Drug Discov 2021 Jun 11. Epub 2021 Jun 11.

Department of Pathology, Massachusetts General Research Institute, Boston, MA, USA.

Numerous drug treatments that have recently entered the clinic or clinical trials have their genesis in zebrafish. Zebrafish are well established for their contribution to developmental biology and have now emerged as a powerful preclinical model for human disease, as their disease characteristics, aetiology and progression, and molecular mechanisms are clinically relevant and highly conserved. Zebrafish respond to small molecules and drug treatments at physiologically relevant dose ranges and, when combined with cell-specific or tissue-specific reporters and gene editing technologies, drug activity can be studied at single-cell resolution within the complexity of a whole animal, across tissues and over an extended timescale. Read More

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Aspects of tree shrew consolidated sleep structure resemble human sleep.

Commun Biol 2021 Jun 11;4(1):722. Epub 2021 Jun 11.

Department of Neuroscience and Movement Sciences, Section of Medicine, University of Fribourg, Fribourg, Switzerland.

Understanding human sleep requires appropriate animal models. Sleep has been extensively studied in rodents, although rodent sleep differs substantially from human sleep. Here we investigate sleep in tree shrews, small diurnal mammals phylogenetically close to primates, and compare it to sleep in rats and humans using electrophysiological recordings from frontal cortex of each species. Read More

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Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue.

Sci Rep 2021 Jun 11;11(1):12409. Epub 2021 Jun 11.

Veterinary Pharmacology and Stem Cell Research Laboratory, Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.

The trend of regenerative therapy for diabetes in human and veterinary practices has conceptually been proven according to the Edmonton protocol and animal models. Establishing an alternative insulin-producing cell (IPC) resource for further clinical application is a challenging task. This study investigated IPC generation from two practical canine mesenchymal stem cells (cMSCs), canine bone marrow-derived MSCs (cBM-MSCs) and canine adipose-derived MSCs (cAD-MSCs). Read More

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Engineered bridge protein with dual affinity for bone morphogenetic protein-2 and collagen enhances bone regeneration for spinal fusion.

Sci Adv 2021 Jun 11;7(24). Epub 2021 Jun 11.

Pritzker School of Molecular Engineering, University of Chicago, Chicago, IL 60637, USA.

The revolutionizing efficacy of recombinant human bone morphogenetic protein (rhBMP-2) for clinical spinal fusion is hindered by safety issues associated with the high dose required. However, it continues to be widely used, for example, in InFUSE Bone Graft (Medtronic). Here, we developed a translational protein engineering-based approach to reduce the dose and thereby improve the safety of rhBMP-2 delivered in a collagen sponge, as in InFUSE Bone Graft. Read More

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Management of Human and Animal Bites.

Oral Maxillofac Surg Clin North Am 2021 Jun 8. Epub 2021 Jun 8.

Oral and Maxillofacial Surgery, Cook County Health, Chicago, IL, USA; Midwestern University, Chicago, IL, USA. Electronic address:

Dogs are the animal most frequently implicated in causing bite injuries to the human face. Dog bite injuries are most prevalent in younger patients. Pasteurella species are commensals of the oral microbiome of dogs and cats and are frequently implicated in infections resulting from dog and cat bite injuries. Read More

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Downregulation of decidual SKP2 is associated with human recurrent miscarriage.

Reprod Biol Endocrinol 2021 Jun 11;19(1):88. Epub 2021 Jun 11.

Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200135, China.

Background: Recurrent miscarriage (RM) is a very frustrating problem for both couples and clinicians. To date, the etiology of RM remains poorly understood. Decidualization plays a critical role in implantation and the maintenance of pregnancy, and its deficiency is closely correlated with RM. Read More

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Mercury in wild animals and fish and health risk for indigenous Amazonians.

Food Addit Contam Part B Surveill 2021 Jun 11:1-9. Epub 2021 Jun 11.

Post Graduation Program in Regional Development and Environment, Federal University of Rondônia, Porto Velho, Brazil.

Total mercury (T-Hg) was determined in fish and wild animal meat consumed in indigenous villages in the Brazilian Amazon region, where there is no history of gold mining. The analyses were performed in an atomic absorption spectrophotometer by generation of cold vapour. Regardless of the dietary habit, 42. Read More

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Analysis of allergen profile in patients sensitized to canine allergen and potential Can f 5 cross-reactivity with human PSA.

Int J Immunopathol Pharmacol 2021 Jan-Dec;35:20587384211023670

Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland.

Can f 5 allergy and possible cross-reactivity with human semen in which there are significant amounts of prostate-specific antigen (PSA) are particularly interesting aspects of allergy to dog. The objective of the study was to confirm cross-reactivity between human PSA and Can f 5 in a study of canine sensitised women. A total of 100 women (aged 18-73, 41 on average) with a positive history of animal fur allergy or positive skin prick tests to canine allergens were selected. Read More

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Monte Carlo determination of dose coefficients at different developmental stages of zebrafish (Danio rerio) in experimental condition.

J Environ Radioact 2021 Jun 8;237:106667. Epub 2021 Jun 8.

State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, 215123, China; Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions. Electronic address:

The release of liquid effluent of nuclear power into aquatic system increases with the rapid development of nuclear facilities in coastal and inland regions. Aquatic model animals are very important for the study of the radiation hazards to non-human biota in water environment and its extrapolation of dose-effect relationship to human models. However, the study of the radiation dose rate calculation model of the aquatic animal zebrafish is still on the homogeneous isotropic model used for the protection of the environment. Read More

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Potentials of single-cell genomics in deciphering cellular phenotypes.

Curr Opin Plant Biol 2021 Jun 8;63:102059. Epub 2021 Jun 8.

Center for Genomics and Systems Biology, Department of Biology, New York University, 12 Waverly Pl, New York, NY 10003, United States. Electronic address:

Single-cell genomics, particularly single-cell transcriptome profiling by RNA sequencing have transformed the possibilities to relate genes to functions, structures, and eventually phenotypes. We can now observe changes in each cell's transcriptome and among its neighborhoods, interrogate the sequence of transcriptional events, and assess their influence on subsequent events. This paradigm shift in biology enables us to infer causal relationships in these events with high accuracy. Read More

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Effect of azithromycin on bronchial wall thickness in severe persistent asthma: A double-blind placebo-controlled randomized clinical trial.

Respir Med 2021 Jun 2;185:106494. Epub 2021 Jun 2.

Department of Radiology, Mashhad Medical Sciences Branch, Islamic Azad University, Mashhad, Iran. Electronic address:

Background: Azithromycin reduced airway remodeling in animal models of asthma. However, its effect on human subjects has not been studied yet. This study aimed to investigate the effect of long-term treatment with azithromycin on airways wall thickness in patients with severe persistent asthma. Read More

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In vitro and in vivo study on angiogenesis of porcine induced pluripotent stem cell-derived endothelial cells.

Differentiation 2021 Jun 4;120:10-18. Epub 2021 Jun 4.

College of Life Science, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address:

Pluripotent stem cells (PSCs) are a promising source of endothelial cells (ECs) for the treatment of cardiovascular diseases. Since clinical application of embryo stem cells (ESCs) involves issues of medical ethics and risk of immune rejection, induced pluripotent stem cells (iPSCs) will facilitate cell transplantation therapy for the cardiovascular diseases. Swine is identified as an ideal large-animal model for human, because of its similar organ size and physiological characteristics. Read More

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Creating Clinically Relevant Aneurysm Sizes in the Rabbit Surgical Elastase Model.

World Neurosurg 2021 Jun 8. Epub 2021 Jun 8.

Hotchkiss Brain Institute, University of Calgary, Alberta, Canada; Department of Radiology, Foothills Medical Centre, Calgary, Alberta; Department of Clinical Neurosciences, Foothills Medical Centre, Calgary, Alberta. Electronic address:

Background: Creating aneurysm sizes in animal models that resemble human aneurysms is essential to study and test neuroendovascular devices. The commonly used rabbit surgical elastase model, however, produces saccular aneurysms that are smaller than those typically treated in humans. The goal of this study was to determine whether an increased vessel stump length and the addition of calcium chloride to the incubation solution has an effect on the resulting aneurysm size. Read More

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Restriction of Iron Loading into Developing Seeds by A YABBY Transcription Factor Safeguards Successful Reproduction in Arabidopsis.

Mol Plant 2021 Jun 8. Epub 2021 Jun 8.

State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China;. Electronic address:

Iron (Fe) storage in plant seeds is not only necessary for seedling establishment following germination, but also a major source of dietary Fe for human and animal life. Seed Fe accumulation was known to be low during early seed development. However, the underlying mechanism and biological significance remain elusive. Read More

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