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Recent applications of mass spectrometry for the characterization of cannabis and hemp phytocannabinoids: From targeted to untargeted analysis.

J Chromatogr A 2021 Aug 25;1655:462492. Epub 2021 Aug 25.

Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, Rome 00185, Italy; CNR NANOTEC, Campus Ecotekne, University of Salento, Via Monteroni, Lecce 73100, Italy.

This review is a collection of recent applications of mass spectrometry studies for the characterization of phytocannabinoids in cannabis and hemp plant material and related products. The focus is mostly on recent applications using mass spectrometry as detector, in hyphenation to typical separation techniques (i.e. Read More

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Gas exchange measurements in the unsteady state.

Plant Cell Environ 2021 Sep 4. Epub 2021 Sep 4.

LI-COR Biosciences, Lincoln, Nebraska, USA.

Leaf level gas exchange is a widely used technique that provides real-time measurement of leaf physiological properties, including CO assimilation (A), stomatal conductance to water vapour (g ) and intercellular CO (C ). Modern open-path gas exchange systems offer greater portability than the laboratory-built systems of the past and take advantage of high-precision infrared gas analyzers and optimized system design. However, the basic measurement paradigm has long required steady-state conditions for accurate measurement. Read More

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September 2021

Gycyrrhizic acid alleviates atherosclerotic lesions in rats with diabetes mellitus.

Mol Med Rep 2021 Nov 3;24(5). Epub 2021 Sep 3.

Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China.

Gycyrrhizic acid (GA), an inhibitor of high mobility group box 1 (HMGB1), inhibits inflammatory responses and is involved in the occurrence and development of several inflammation‑related diseases. However, the role of GA in the atherosclerotic lesions caused by diabetes mellitus (DM) remains unknown. In the present study, Sprague Dawley rats were selected to desi=gn a diabetic atherosclerosis (AS) model. Read More

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November 2021

Spherical micro-hole grid for high-resolution retarding field analyzer.

J Synchrotron Radiat 2021 Sep 19;28(Pt 5):1669-1671. Epub 2021 Aug 19.

Research Organization for Nano and Life Innovation, Waseda University, 513 Wasedatsurumaki, Shinjuku, Tokyo 162-0041, Japan.

A wide-acceptance-angle spherical grid composed of numerous micro cylindrical holes was developed to be used for the retarding grid of a display-type retarding field analyzer (RFA) and to enhance the energy resolution (E/ΔE). Each cylindrical hole with a diameter of 50 µm and a depth of 80 µm is directed to the spherical center. The inner radius of the spherical grid is 40 mm. Read More

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September 2021

Voltage standing wave ratio reading circuit design for inductance capacitance wireless passive ammonia sensors.

Rev Sci Instrum 2021 Aug;92(8):085003

Science and Technology on Electronic Test and Measurement Laboratory, North University of China, Taiyuan 030051, China.

It is not easy to conduct wired tests on sensors in harsh environments, and network analyzers are large, heavy, and inconvenient to carry. At the same time, the price of network analyzers is usually very high, which greatly limits their application. In this study, a voltage standing wave ratio reading circuit is designed to test inductively coupled (LC) wireless passive sensors. Read More

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Planning SQC strategies and adapting QC frequency for patient risk.

Clin Chim Acta 2021 Aug 28;523:1-5. Epub 2021 Aug 28.

Westgard QC, Inc., Madison, WI, USA.

Background: Risk-based Statistical QC strategies are recommended by the CLSI guidance for Statistical Quality Control (C24-Ed4). Using Parvin's patient risk model, QC frequency can be determined in terms of run size, i.e. Read More

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Mobile diagnostics and consultation for the prevention of the metabolic syndrome and its secondary diseases in Brandenburg-study protocol of a regional prospective cohort study: the Mobile Brandenburg Cohort.

Pilot Feasibility Stud 2021 Aug 30;7(1):166. Epub 2021 Aug 30.

University Outpatient Clinic, Sports Medicine and Sports Orthopedics, University of Potsdam, Am Neuen Palais 10, 14469, Potsdam, Germany.

Background: The metabolic syndrome (MetS) is a risk cluster for a number of secondary diseases. The implementation of prevention programs requires early detection of individuals at risk. However, access to health care providers is limited in structurally weak regions. Read More

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Sysmex UF-5000 Automatic Urine Sediment Analyzer Can Improve the Accuracy of Epithelial Cell Detection.

Ann Clin Lab Sci 2021 Jul;51(4):562-569

Department of Clinical Laboratory Medicine, Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi'an, Shaanxi, China

Objective: The aim of this study was to evaluate the consistency and accuracy of all the parameters of the urine samples detected by two automated urine sediment analyzers from Sysmex Corporation.

Methods: Two automated analyzers and manual microscopy examined 1,059 urine samples. The sensitivity, specificity, positive predictive value, and negative predictive value were evaluated. Read More

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The validity and reliability of computer-aided semen analyzers in performing semen analysis: a systematic review.

Transl Androl Urol 2021 Jul;10(7):3069-3079

American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.

Background: Computer-aided sperm analyzers (CASA) are currently used worldwide for semen analysis. However, there are doubts about their reliability to fully substitute the human operator. Therefore, this study aimed to systematically review the current literature comparing results from semen evaluation by both CASA-based and manual approaches. Read More

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High-Resolution Native Mass Spectrometry.

Chem Rev 2021 Aug 20. Epub 2021 Aug 20.

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Native mass spectrometry (MS) involves the analysis and characterization of macromolecules, predominantly intact proteins and protein complexes, whereby as much as possible the native structural features of the analytes are retained. As such, native MS enables the study of secondary, tertiary, and even quaternary structure of proteins and other biomolecules. Native MS represents a relatively recent addition to the analytical toolbox of mass spectrometry and has over the past decade experienced immense growth, especially in enhancing sensitivity and resolving power but also in ease of use. Read More

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Reliability of knee extensor neuromuscular structure and function and functional tests' performance.

J Bodyw Mov Ther 2021 Jul 12;27:584-590. Epub 2021 May 12.

Exercise Research Laboratory, School of Physical Education, Physiotherapy and Dance, Federal University of Rio Grande do Sul - Porto Alegre, Rio Grande do Sul, Brazil.

Introduction: The aim of this study was to evaluate the intra and inter-rater and inter-analyzer reliability of neuromuscular variables and functional tests.

Methods: Cross-sectional crossover design. Two independent raters and analyzers evaluated twenty-two healthy subjects. Read More

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Hemoglobin quantification in red blood cells via dry mass mapping based on UV absorption.

J Biomed Opt 2021 Aug;26(8)

Georgia Institute of Technology & Emory Univ. School of Medicine, United States.

Significance: The morphological properties and hemoglobin (Hb) content of red blood cells (RBCs) are essential biomarkers to diagnose or monitor various types of hematological disorders. Label-free mass mapping approaches enable accurate Hb quantification from individual cells, serving as promising alternatives to conventional hematology analyzers. Deep ultraviolet (UV) microscopy is one such technique that allows high-resolution, molecular imaging, and absorption-based mass mapping. Read More

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Repeatability and comparability of the Galilei-G4 and Cassini in measuring corneal power and astigmatism in normal and post-refractive surgery eyes.

Sci Rep 2021 08 9;11(1):16141. Epub 2021 Aug 9.

Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, 6565 Fannin, NC205, Houston, TX, 77030, USA.

To assess the repeatability and comparability of the Galilei G4 versus the Cassini topographer in post-refractive eyes and in normal eyes, including older patients representative of an initial cataract evaluation. Simulated keratometric (simK), total corneal and posterior corneal power and astigmatism were evaluated in both post-refractive and normal eyes. Repeatability was measured by calculating within-subject standard deviation (Sw), coefficient of variation (CoV), and intraclass correlation coefficient (ICC). Read More

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Evaluation of femtosecond laser-induced breakdown spectroscopy system as an offline coal analyzer.

Sci Rep 2021 Aug 5;11(1):15968. Epub 2021 Aug 5.

State Key Lab of Power Systems, Department of Energy and Power Engineering, International Joint Lab on Low Carbon Clean Energy Innovation, Tsinghua University, Beijing, 100084, China.

Developments in femtosecond laser induced breakdown spectroscopy (fs-LIBS) applications during the last two decades have further centered on innovative métier tie-in to the advantageous properties of femtosecond laser ablation (fs-LA) introduced into LIBS. Yet, for industrially-oriented application like coal analysis, no research has exposed to view the analytical capabilities of fs-LA in enhancing the physical processes of coal ablation and the impact into quantitative correlation of spectra and data modeling. In a huge coal market, fast and accurate analysis of coal property is eminently important for coal pricing, combustion optimization, and pollution reduction. Read More

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The association of air pollutants exposure with subclinical inflammation and carotid atherosclerosis.

Int J Cardiol 2021 Nov 31;342:108-114. Epub 2021 Jul 31.

1st Cardiology Clinic, 'Hippokration' General Hospital, National and Kapodistrian University of Athens, School of Medicine, Athens, Greece.

Background: Air pollution is a well-described environmental factor with evidence suggesting a firm association with cardiovascular diseases. The purpose of this study was to determine the association of exposure to gaseous air pollutants on atherosclerosis burden.

Methods: 1955 inhabitants of the Corinthia region, aged 40 years or older, underwent clinical and biochemical assessment as well as carotid ultrasonography to evaluate carotid intima-media thickness (cIMT) and plaque burden. Read More

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November 2021

Role of immature platelet fraction (IPF) in sepsis patients: A systematic review.

J Family Med Prim Care 2021 Jun 2;10(6):2148-2152. Epub 2021 Jul 2.

Internal Medicine Department, Creighton University Hospital Program, Omaha, Nebraska, USA.

Sepsis is extremely common amongst critically ill patients and requires early diagnosis. Hence, identifying a biomarker that could acknowledge sepsis at its prior stage is of vital significance. Immature platelets are a percentage of circulating platelets that contain RNA and is a newer parameter that is measured using automated hematology analyzers in diagnosing sepsis. Read More

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Comparison of Unit Resolution Versus High-Resolution Accurate Mass for Parallel Reaction Monitoring.

J Proteome Res 2021 Sep 28;20(9):4435-4442. Epub 2021 Jul 28.

Department of Genome Sciences, University of Washington, 3720 15th Street NE, Seattle, Washington 98195, United States.

Parallel reaction monitoring (PRM) is an increasingly popular alternative to selected reaction monitoring (SRM) for targeted proteomics. PRM's strengths over SRM are that it monitors all product ions in a single spectrum, thus eliminating the need to select interference-free product ions prior to data acquisition, and that it is most frequently performed on high-resolution instruments, such as quadrupole-orbitrap and quadrupole-time-of-flight instruments. Here, we show that the primary advantage of PRM is the ability to monitor all transitions in parallel and that high-resolution data are not necessary to obtain high-quality quantitative data. Read More

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September 2021

Improving the sensitivity of cellulose fiber-based lateral flow assay by incorporating a water-dissolvable polyvinyl alcohol dam.

Cellulose (Lond) 2021 Jul 17:1-11. Epub 2021 Jul 17.

Department of Chemical Engineering, University of New Brunswick, Fredericton, NB E3B 5A3 Canada.

Lateral flow assay (LFA) is an important point-of-care (POC) test platform due to the associated portability, on-site testing, and low cost for diagnosis of pathogen infections and disease biomarkers. However, compared to high-end analyzers in hospitals, LFA devices, in particular, paper- based LFA tests, fall short in accuracy. This study focuses on two ways to improve LFAs: (1) using cellulose fibers, rather than glass fibers for a sample pad, and (2) incorporating a one-step simple, facile, and low cost PVA dam into the LFA. Read More

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Assessment of the applicability of a low-cost sensor-based methane monitoring system for continuous multi-channel sampling.

Environ Monit Assess 2021 Jul 23;193(8):509. Epub 2021 Jul 23.

Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama, 338-8570, Japan.

Systems that are made of several low-cost gas sensors with automatic gas sampling may have the potential to serve as reliable fast methane analyzers. However, there is a lack of reports about such types of systems evaluated under field conditions. Here, we developed a continuous methane monitoring system with automated gas sampling unit using low-cost gas sensors, TGS 2611 and MQ-4, that use a simple cloud-based data acquisition platform. Read More

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Disruptive innovations in the clinical laboratory: catching the wave of precision diagnostics.

Crit Rev Clin Lab Sci 2021 Jul 23:1-17. Epub 2021 Jul 23.

Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, Canada.

Disruptive innovation is an invention that disrupts an existing market and creates a new one by providing a different set of values, which ultimately overtakes the existing market. Typically, when disruptive innovations are introduced, their performance is initially less than existing standard technologies, but because of their ability to bring the cost down, and with gradual improvement, they end up replacing established service standards.Disruptive technologies have their fingerprints in health care. Read More

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Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity.

Sci Rep 2021 Jul 22;11(1):14978. Epub 2021 Jul 22.

Department of Laboratory Medicine, National Cancer Center Hospital, 5-1-1, Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan.

The chylous turbidity of blood samples is one of the causes of false-high hemoglobin (Hgb) concentration measurements by the colorimetric method, which has been widely applied in hematology analyzers. In such cases, additional manual procedures are required to correct Hgb concentrations. We therefore examined the effectiveness of an optical method for measuring Hgb concentrations in samples with chylous turbidity using Hgb-O in the reticulocyte channel equipped in XN-series analyzers (Sysmex, Kobe, Japan). Read More

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Mass spectrometry-based methods for the advanced characterization and structural analysis of lignin: A review.

Mass Spectrom Rev 2021 Jul 22. Epub 2021 Jul 22.

Department of Chemistry, Humboldt University Berlin, Berlin, Germany.

Lignin is currently one of the most promising biologically derived resources, due to its abundance and application in biofuels, materials and conversion to value aromatic chemicals. The need to better characterize and understand this complex biopolymer has led to the development of many different analytical approaches, several of which involve mass spectrometry and subsequent data analysis. This review surveys the most important analytical methods for lignin involving mass spectrometry, first looking at methods involving gas chromatography, liquid chromatography and then continuing with more contemporary methods such as matrix assisted laser desorption ionization and time-of-flight-secondary ion mass spectrometry. Read More

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Evaluation of Sigma metric approach for monitoring the performance of automated analyzers in hematology unit of Alexandria Main University Hospital.

Int J Lab Hematol 2021 Jul 18. Epub 2021 Jul 18.

Department of Clinical and Chemical pathology, Faculty of Medicine, University of Alexandria, Azarita Medical Campus, Alexandria, Egypt.

Introduction: Sigma metric offers a quantitative framework for evaluating process performance in clinical laboratories. This study aimed to evaluate the analytical performance of automated analyzers in hematology unit of Alexandria Main University Hospital using the sigma metric approach.

Materials And Methods: Quality control data were collected for 6 months, and sigma value was calculated from hematology analyzers SYSMEX (XN 1000, XT 1800i), ADVIA (2120i, 2120), and coagulation analyzers SYSMEX CA 1500 (3610, 6336). Read More

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An artificial intelligence-assisted diagnostic platform for rapid near-patient hematology.

Am J Hematol 2021 10 2;96(10):1264-1274. Epub 2021 Aug 2.

Department of Laboratory Medicine, Boston Children's Hospital, Boston, Massachusetts, USA.

Hematology analyzers capable of performing complete blood count (CBC) have lagged in their prevalence at the point-of-care. Sight OLO (Sight Diagnostics, Israel) is a novel hematological platform which provides a 19-parameter, five-part differential CBC, and is designed to address the limitations in current point-of-care hematology analyzers using recent advances in artificial intelligence (AI) and computer vision. Accuracy, repeatability, and flagging capabilities of OLO were compared with the Sysmex XN-Series System (Sysmex, Japan). Read More

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October 2021

Analytical Performance and Comparability of Three New Coagulation Analyzers.

Clin Lab 2021 Jul;67(7)

Background: Analytical performance should be evaluated before a new coagulation analyzer is adopted in a clinical laboratory. The objective of this study was to evaluate analytical performances of three new coagulation analyzers (STA-R Max3, CN-6000, and Cobas t511) and compare them based on the following four coagulation parameters: prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen, and D-dimer.

Methods: A total of 427 plasma samples, including fresh and frozen/thawed plasma spanning wide ranges. Read More

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A machine learning PROGRAM to identify COVID-19 and other diseases from hematology data.

Future Sci OA 2021 Aug 12;7(7):FSO733. Epub 2021 Jun 12.

Sysmex New Zealand Ltd, Auckland, New Zealand.

Aim: We propose a method for screening full blood count metadata for evidence of communicable and noncommunicable diseases using machine learning (ML).

Materials & Methods: High dimensional hematology metadata was extracted over an 11-month period from Sysmex hematology analyzers from 43,761 patients. Predictive models for age, sex and individuality were developed to demonstrate the personalized nature of hematology data. Read More

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Software Options for the Analysis of MS-Proteomic Data.

Methods Mol Biol 2021 ;2361:35-59

Department of Experimental Oncology, European Institute of Oncology (IEO), IRCCS, Milan, Italy.

Mass spectrometry (MS)-based proteomics is currently the most successful approach to measure and compare peptides and proteins in a large variety of biological samples. Modern mass spectrometers, equipped with high-resolution analyzers, provide large amounts of data output. This is the case of shotgun/bottom-up proteomics, which consists in the enzymatic digestion of protein into peptides that are then measured by MS-instruments through a data dependent acquisition (DDA) mode. Read More

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January 2021

[Spectrum peak detection algorithm based on trend accumulation without base deduction].

Se Pu 2021 Jun;39(6):670-677

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China.

The detection and analysis of spectral peaks play an important role in research on chromatography technology. However, in the process of collecting and transmitting chromatographic data, it is very difficult to detect spectral peaks owing to the interference of different levels of noise. Most of the traditional spectral peak detection algorithms follow three steps: spectral smoothing, baseline correction, and spectral peak recognition, which require high denoising and curve smoothing, and therefore increase the complexity of the algorithm. Read More

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Clinical performance evaluation of Elecsys HIV Duo, Anti-HCV II, HBsAg II, Anti-HBc II, and Syphilis assays for routine screening of first-time blood donor samples at a French blood donation center.

Transfus Clin Biol 2021 Jun 30. Epub 2021 Jun 30.

Etablissement Français du Sang, Provence Alpes-Côte d'Azur, 149, boulevard Baille, 13005 Marseille, France.

Objectives: Implementing fully automated analyzers has become a crucial safety step in blood donation centers. The Elecsys® assays were evaluated on the cobas e 801 module (Roche Diagnostics) for routine first-time blood donor screening.

Materials & Methods: Five Elecsys infectious disease assays were tested on the cobas e 801 module at Etablissement Français du Sang, Montpellier, France (March-April 2018). Read More

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Polarization effects of X-ray monochromators modeled using dynamical scattering theory.

Acta Crystallogr A Found Adv 2021 Jul 27;77(Pt 4):262-267. Epub 2021 May 27.

Materials Measurement Laboratory, NIST, US Department of Commerce, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.

The difference in the diffracted intensity of the σ- and π-polarized components of an X-ray beam in powder diffraction has generally been treated according to equations based on dipole scattering, also known as kinematic X-ray scattering. Although this treatment is correct for powders and post-sample analyzers known to be of high mosaicity, it does not apply to systems configured with nearly perfect crystal incident-beam monochromators. Equations are presented for the polarization effect, based on dynamical diffraction theory applied to the monochromator crystal. Read More

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