3,999 results match your criteria analyzed immunohistochemically


Cancer-associated fibroblasts in gastric cancer affect malignant progression via the CXCL12-CXCR4 axis.

J Cancer 2021 19;12(10):3011-3023. Epub 2021 Mar 19.

Department of Pathology, Medical College of Soochow University, Soochow University, Suzhou, China.

Cancer-associated fibroblasts (CAFs) are principal constituents of the tumor microenvironment (TME) and play a critical role in tumor progression. The CXCL12/CXCR4 axis regulates multiple facets of the TME. The aim of this study was to determine the relationship between CXCL12 expression in CAFs and the malignant progression of gastric cancer (GC). Read More

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Breast adipose tissue macrophages (BATMs) have a stronger correlation with breast cancer survival than breast tumor stroma macrophages (BTSMs).

Breast Cancer Res 2021 Apr 13;23(1):45. Epub 2021 Apr 13.

Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Marchioninistr. 15, 81377, Munich, Germany.

Background: An abundance of tumor-associated macrophages has been shown to be an independent prognostic factor for a poor prognosis of human breast cancer (BC). Adipose tissue accounts for the largest proportion of the breast and has also been identified as an independent indicator of poor survival in BC. This study aims to elucidate if the influence of adipose tissue in BC might be mediated by macrophages. Read More

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Different role of MSLN and CA125 co-expression as a prognostic predictor between perihilar and distal bile duct carcinoma.

Oncol Lett 2021 May 23;21(5):414. Epub 2021 Mar 23.

Department of Surgery, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.

Recent studies have suggested that the interaction of mesothelin (MSLN) and cancer antigen 125 (CA125) enhances tumor metastases. The aim of the present study was to clarify the impact of MSLN and CA125 co-expression on the prognosis of patients with extrahepatic bile duct carcinoma (BDC). Tissue samples from patients who underwent surgical resection between 2007 and 2015 for perihilar or distal BDC were immunohistochemically examined. Read More

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Inhibition of STAT3 prevents bone metastatic progression of prostate cancer in vivo.

Prostate 2021 Apr 6. Epub 2021 Apr 6.

Section for Oncology, Department of Radiation Sciences, Umeå University, Umeå, Sweden.

Background: Prostate cancer (PC) metastasizes to the skeleton forming predominantly sclerotic lesions, and there is currently no cure for bone metastatic disease. The transcription factor signal transducer and activator of transcription 3 (STAT3) is implicated as a metastatic driver, but its potential as therapeutic target in bone metastasis has not been investigated. In this study, we evaluated for the first time a STAT3 inhibitor, Napabucasin, as a therapeutic option for bone metastatic PC. Read More

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An Efficacy Study of a New Radical Treatment for Acne Vulgaris Using Fat Injection.

Aesthet Surg J 2021 Apr 2. Epub 2021 Apr 2.

Center of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Harbin Medical University, Nangang, Harbin, People's Republic of China.

Background: Fat grafting is a popular operative approach for rejuvenation. Some patients needing facial fat grafting also have acne, a common disorder with a high incidence. Fat grafting may improve areas with acne in some patients. Read More

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Comparison of clinical outcomes and FOXP3, IL-17A responses in Helicobacter pylori infection in children versus adults.

Helicobacter 2021 Apr 5:e12795. Epub 2021 Apr 5.

Faculty of Medicine, Pediatrics, Kırıkkale University, Kırıkkale, Turkey.

Background: The purpose of this study was to compare the clinical symptoms and pathological consequences of Helicobacter pylori (H. pylori) infection between children and adults and determine the levels of expression of FOX3P and IL-17A to examine the Th17/Treg balance.

Methods: Forty pediatric and 40 adult patients who were followed up at the Pediatric Gastroenterology and Internal Medicine Gastroenterology Departments were enrolled in the study. Read More

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Comparative clinicopathological aspects of chronic tonsillitis and adenoiditis in children.

Rom J Morphol Embryol 2020 Jul-Sep;61(3):895-904

Department of Infant Care-Pediatrics-Neonatology, Discipline of Pediatrics, Department of Histology, University of Medicine and Pharmacy of Craiova, Romania;

Chronic palatal and nasopharyngeal inflammations are common lesions in pediatric pathology, with major effects on children's development. The study included 34 cases of chronic tonsillitis and adenoiditis for which we quantified immunohistochemically and analyzed the distribution of inflammatory elements in the follicular, extrafollicular and epithelial compartments, in relation to the composite histological scores and the clinico-epidemiological profile of the lesions. The cases were more frequent under the age of 10, in female patients, coming from urban areas, with the diagnosis of tonsillitis. Read More

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Involvement of inflammatory cells in chronic rhinosinusitis with nasal polyps.

Rom J Morphol Embryol 2020 Jul-Sep;61(3):871-877

Department of ENT, University of Medicine and Pharmacy of Craiova, Romania;

Inflammation plays an important role in the pathogenesis of nasal polyps. Understanding the biomolecular action mechanisms of inflammatory elements can contribute to improving the prognosis of these lesions. The study analyzed the distribution and immunohistochemically quantified eosinophils [eosinophil major basic protein (BMK-13)], lymphocytes [cluster of differentiation (CD) 4, CD8, CD20] and plasmocytes (CD138) in both the epithelial and stromal compartment in relation to composite scores, which included specific histopathological parameters for 50 sinonasal polyps. Read More

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Integrin α2β1 Represents a Prognostic and Predictive Biomarker in Primary Ovarian Cancer.

Biomedicines 2021 Mar 12;9(3). Epub 2021 Mar 12.

Department of General, Visceral and Transplant Surgery, University Hospital, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377 Munich, Germany.

Currently, the same first-line chemotherapy is administered to almost all patients suffering from primary ovarian cancer. The high recurrence rate emphasizes the need for precise drug treatment in primary ovarian cancer. Being crucial in ovarian cancer progression and chemotherapeutic resistance, integrins became promising therapeutic targets. Read More

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High vimentin expression with E-cadherin expression loss predicts a poor prognosis after resection of grade 1 and 2 pancreatic neuroendocrine tumors.

BMC Cancer 2021 Mar 31;21(1):334. Epub 2021 Mar 31.

Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.

Background: Pancreatic neuroendocrine tumors (pNETs) are a heterogeneous group of neoplasms with malignant behaviors that can develop from inert slow growth or low malignancy to aggressive metastasis during follow-up. Recently, vimentin and E-cadherin were shown to be prognostic markers in some malignant tumors but were not evaluated in pNETs. The aim of this study was to evaluate the expression and prognostic significance of vimentin and E-cadherin in grade 1 and 2 pNETs. Read More

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Up-regulation of HDAC6 Results in Poor Prognosis and Chemoresistance in Patients With Advanced Ovarian High-grade Serous Carcinoma.

Anticancer Res 2021 Mar;41(3):1647-1654

Department of Pathology, Saitama Medical University International Medical Center, Saitama, Japan;

Background: Ovarian high-grade serous carcinoma (HGSC) gradually acquires chemoresistance after recurrence. Our previous study on ovarian clear-cell carcinoma found histone deacetylase 6 (HDAC6) overexpression led to chemoresistance. This study aimed to evaluate HDAC6 as a predictor of chemoresistance and a therapeutic target for ovarian HGSC. Read More

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Multicohort Retrospective Validation of a Predictive Biomarker for Topoisomerase I Inhibitors.

Clin Colorectal Cancer 2020 Dec 10. Epub 2020 Dec 10.

Division of Hematology Oncology, Department of Medicine, Boston University School of Medicine, Boston, MA. Electronic address:

Purpose: The camptothecin (CPT) analogs topotecan and irinotecan specifically target topoisomerase I (topoI) and are used to treat colorectal, gastric, and pancreatic cancer. Response rate for this class of drug varies from 10% to 30%, and there is no predictive biomarker for patient stratification by response. On the basis of our understanding of CPT drug resistance mechanisms, we developed an immunohistochemistry-based predictive test, P-topoI-Dx, to stratify the patient population into those who did and did not experience a response. Read More

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December 2020

Glypican-1 is a novel immunohistochemical marker to differentiate poorly differentiated squamous cell carcinoma from solid predominant adenocarcinoma of the lung.

Transl Lung Cancer Res 2021 Feb;10(2):766-775

Department of Pathology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.

Background: The histological classification of non-small cell lung cancer (NSCLC) is essential in determining new cancer-specific targeted therapies. However, the accurate typing of poorly differentiated is difficult, particularly for poorly differentiated squamous cell carcinoma and adenocarcinoma of the lung with limited immunohistochemical markers. Thus, novel immunohistochemical markers are required. Read More

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February 2021

The expression patterns and prognostic significance of pleckstrin homology-like domain family A (PHLDA) in lung cancer and malignant mesothelioma.

J Thorac Dis 2021 Feb;13(2):689-707

Department of Pathology, University of São Paulo Medical School (USP), São Paulo, Brazil.

Background: Pleckstrin homology domain family A (PHLDA) genes play important roles in cancer cellular processes, including inhibiting Akt activation, repressing growth factor signaling, inhibiting the negative feedback of EGFR/ErbB2 signaling cells, and inducing apoptosis. However, the prognostic significance of PHLDA in non-small cell lung cancer (NSCLC) and malignant pleural mesothelioma (MM) remains unclear. The present study investigates the associations between PHLDA expression patterns and their prognostic value in lung adenocarcinoma (LUAD) and MM. Read More

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February 2021

Cell membrane and nuclear expression of programmed death ligand-1 in prostate needle biopsy tissue in prostate cancer patients undergoing primary radiation therapy.

Urol Oncol 2021 Mar 9. Epub 2021 Mar 9.

Department of Urology, Ajou University School of Medicine, Suwon, South Korea. Electronic address:

Background: Programmed death ligand-1 (PD-L1) expression in cancer is often associated with cancer aggressiveness and responsiveness to treatment with PD-1 pathway inhibitors. We conducted a systematic study on the expression of membranous PD-L1 (mPD-L1) and nuclear PD-1-L1 (nPD-L1) in prostate needle biopsy specimens of prostate cancer patients who underwent primary radiotherapy and analyzed the association between PD-L1 expression and clinicopathological characteristics and prognosis of patients.

Method: A total of 971 cancer-containing prostate needle biopsy cores from 172 patients were immunohistochemically stained with anti-PD-L1 antibody. Read More

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Sialadenoma papilliferum: clinicopathologic, Immunohistochemical, molecular analyses of new five cases and review of the literature.

Diagn Pathol 2021 Mar 12;16(1):22. Epub 2021 Mar 12.

Department of Pathology; Key Laboratory of Cancer Prevention and Therapy; Tianjin's Clinical Research Center for Cancer; National Clinical Research Center for Cancer, Tianjin Cancer Institute and Hospital, Tianjin Medical University, Tianjin, PR China.

Background: Sialadenoma papilliferum (SP) is an extremely rare benign neoplasm of salivary glands. To explore and define the clinicopathological features of SP, we retrospectively analyzed 89 cases previously reported and five new cases.

Methods: The clinical features, histopathology, immunohistochemistry and molecular analysis of our cases were further performed and the related literatures were reviewed and analyzed. Read More

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G Protein-Coupled Estrogen Receptor Correlates With Dkk2 Expression and Has Prognostic Impact in Ovarian Cancer Patients.

Front Endocrinol (Lausanne) 2021 19;12:564002. Epub 2021 Feb 19.

Department of Obstetrics and Gynecology, University Hospital, Ludwig-Maximilians-University (LMU) Munich, Munich, Germany.

Purpose: Wnt pathway modulator Dickkopf 2 (Dkk2) and signaling of the G protein-coupled estrogen receptor (GPER) seem to have essential functions in numerous cancer types. For epithelial ovarian cancer (EOC), it has not been proven if either Dkk2 or the GPER on its own have an independent impact on overall survival (OS). So far, the correlation of both factors and their clinical significance has not systematically been investigated before. Read More

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February 2021

First Evidence for a Role of Siglec-8 in Breast Cancer.

Int J Mol Sci 2021 Feb 18;22(4). Epub 2021 Feb 18.

Department of Obstetrics and Gynecology, University Hospital, LMU Munich, 80337 Munich, Germany.

Sialic acid-binding immunoglobulin-like lectins (Siglecs) are involved in various immune cell-mediated diseases. Their role in cancer is poorly investigated, and research focusses on Siglec-expression on immune cells interacting with tumor cells. This study evaluates the role of Siglec-8 in breast cancer (BC). Read More

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February 2021

Nanog is a promising chemoresistant stemness marker and therapeutic target by iron chelators for esophageal cancer.

Int J Cancer 2021 Mar 4. Epub 2021 Mar 4.

Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

Esophageal cancer is a disease showing poor prognosis. Although combination chemotherapy using cisplatin (CDDP) and 5-fluorouracil is standard for unresectable esophageal cancer, the response rate is 35%. Cancer stem cells (CSCs) and inflammation are reportedly responsible for the poor prognosis of esophageal cancer. Read More

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Reg4 and its downstream transcriptional activator CD44ICD in stage II and III colorectal cancer.

Oncotarget 2021 Feb 16;12(4):278-291. Epub 2021 Feb 16.

Division of Gastroenterology, Washington University School of Medicine, Saint Louis, MO 63110, USA.

Reg4 is highly expressed in gastrointestinal malignancies and acts as a mitogenic and pro-invasive factor. Our recent works suggest that Reg4 binds with CD44 and induces its proteolytic cleavage to release intra-cytoplasmic domain of CD44 (CD44ICD). The goal of this study is to demonstrate clinical significance of the Reg4-CD44/CD44ICD pathway in stage II/III colon cancer and its association with clinical parameters of aggression. Read More

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February 2021

Investigation of sensory nerve endings in pulvinar, ligamentum teres, and hip joint capsule: A prospective immunohistochemical study of 36 cases with developmental hip dysplasia.

Acta Orthop Traumatol Turc 2021 Jan;55(1):33-37

Department of Orthopaedic Surgery, Harran University, School of Medicine, Şanlıurfa, Turkey.

Objective: The aim of this study was to immunohistochemically identify and characterize the presence of sensory nerve endings (SNEs) in pulvinar, ligamentum teres (LT), and hip joint capsule (HJC) of children with developmental dysplasia of the hip (DDH).

Methods: Pulvinar, LT, and HJC specimens were obtained from 38 hips of 36 children (31 girls, five boys; mean age=49 months; age range=18-132 months) during open reduction surgery for DDH. All specimens underwent subsequent routine tissue processing (formalin fixation and paraffin embedding). Read More

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January 2021

Clinicopathological, Immunohistochemical, and Gene Expression Profiling of Patients with Sporadic Colorectal Cancer.

Arch Iran Med 2021 Feb 1;24(2):86-93. Epub 2021 Feb 1.

Department of Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Background: The DNA mismatch repair (MMR) system is one of the molecular pathways involved in colorectal cancer (CRC) carcinogenesis that consists of several genes, including (MutL homolog 1), (MutS homolog 6), (MutS homolog 2), and (MutS homolog 3). The protein encoded by (post-meiotic segregation 2) is also essential for MMR. Here, we address the correlation between immunohistochemical and transcriptional expression of PMS2 with the tumor grade and clinical stage of non-hereditary/sporadic CRC disease. Read More

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February 2021

Identification of oral squamous cell carcinoma markers MUC2 and SPRR1B downstream of TANGO.

J Cancer Res Clin Oncol 2021 Feb 23. Epub 2021 Feb 23.

Department of Oral and Maxillofacial Surgery, Nara Medical University, Kashihara, Japan.

Purpose: Transport and Golgi organization protein 1 (TANGO) promotes angiogenesis and lymphangiogenesis in oral squamous cell carcinoma (OSCC). To elucidate the underlying mechanisms, this study aims to identify and characterize elements downstream of TANGO that mediate its involvement in OSCC.

Methods: In this study, microarray analysis compared gene expression between control and TANGO-repressed HSC3 cells. Read More

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February 2021

Primary Signet Ring Cell/Histiocytoid Carcinoma of the Eyelid: Somatic Mutations in and Other Clinically Actionable Mutations Imply Early Use of Targeted Agents.

Curr Oncol 2021 Feb 16;28(1):918-927. Epub 2021 Feb 16.

Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei 11217, Taiwan.

Primary signet ring cell/histiocytoid carcinoma of the eyelid is a rare ocular malignancy and its diagnosis is often delayed. This neoplasm presents as an insidious, diffusely infiltrative mass in the periocular area that later infiltrates the orbit. An exenteration is usually indicated; however, nearly one-third of patients develop local recurrence or metastasis. Read More

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February 2021

Eosinophils may serve as CEA-secreting cells for allergic bronchopulmonary aspergillosis (ABPA) patients.

Sci Rep 2021 Feb 17;11(1):4025. Epub 2021 Feb 17.

The First Affiliated Hospital of Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.

Allergic bronchopulmonary aspergillosis (ABPA) is a condition characterized by an exaggerated response of the immune system to the fungus Aspergillus. This study aimed to assess the relationship between carcinoembryonic antigen (CEA) and eosinophils in ABPA patients. We describes a case of a 50-year-old patient who was diagnosed with ABPA presenting with high level of CEA and eosinophils. Read More

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February 2021

Immunohistochemical Expression of MMP-9, TIMP-1, and Vimentin and its Correlation With Inflammatory Reaction and Clinical Parameters in Oral Epithelial Dysplasia.

Appl Immunohistochem Mol Morphol 2021 Feb 9. Epub 2021 Feb 9.

Postgraduate Program in Dentistry Department of Pathology, Health Sciences Center, Federal University of Santa Catarina, Florianópolis, Santa Catarina Postgraduate Program in Business, Federal University of Paraná, Curitiba, Paraná, Brazil.

The aim of this study was to investigate the immunoexpression of matrix metalloproteinase 9 (MMP-9), tissue inhibitor of metalloproteinase 1 (TIMP-1), and vimentin (VIM) and its association with the inflammatory reaction (IR) and clinical parameters in oral epithelial dysplasia (ED). The sample was composed of 66 cases of ED, 27 oral squamous cell carcinoma (OSCC), and 28 non-neoplastic epithelium (NNE). ED was graded according to the binary system as low-risk ED (n=42) and high-risk epithelial dysplasia (HRED: n=24). Read More

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February 2021

Differential Action of Connexin Hemichannel and Pannexin Channel Therapeutics for Potential Treatment of Retinal Diseases.

Int J Mol Sci 2021 Feb 10;22(4). Epub 2021 Feb 10.

School of Optometry and Vision Science, New Zealand National Eye Centre, Centre for Brain Research, Brain Research New Zealand-Rangahau Roro Aotearoa, University of Auckland, Auckland 1142, New Zealand.

Dysregulation of retinal function in the early stages of light-induced retinal degeneration involves pannexins and connexins. These two types of proteins may contribute to channels that release ATP, leading to activation of the inflammasome pathway, spread of inflammation and retinal dysfunction. However, the effect of pannexin channel block alone or block of both pannexin channels and connexin hemichannels in parallel on retinal activity in vivo is unknown. Read More

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February 2021

Roles for hENT1 and dCK in gemcitabine sensitivity and malignancy of meningioma.

Neuro Oncol 2021 Feb 8. Epub 2021 Feb 8.

Department of Molecular Cancer Science, Yamagata University School of Medicine, Yamagata, Japan.

Background: High-grade meningiomas are aggressive tumors with high morbidity and mortality rates that frequently recur even after surgery and adjuvant radiotherapy. However, limited information is currently available on the biology of these tumors and no alternative adjuvant treatment options exist. Although we previously demonstrated that high-grade meningioma cells were highly sensitive to gemcitabine in vitro and in vivo, the underlying molecular mechanisms remain unknown. Read More

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February 2021

Clinical, ultrasound and histopathological correlation of clinically N0 neck nodes in patients with cancers of the pharynx and larynx.

Rom J Morphol Embryol 2020 Apr-Jun;61(2):433-439

Department of Internal Medicine II, Victor Babeş University of Medicine and Pharmacy, Timişoara, Romania; Department of ENT, Carol Davila University of Medicine and Pharmacy, Prof. Dr. Dorin Hociotă Institute of Phonoaudiology and Functional ENT Surgery, Bucharest, Romania;

Background: The presence of metastatic cervical adenopathy is essential for treatment planning and prognosis assessment. Treatment of patients with head and neck cancer with clinically negative cervical lymphadenopathy (N0) remains controversial. Neck palpation, as the method used in tumor, node, metastasis (TNM) staging, has limitations and can provide false negative results in some cases. Read More

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February 2021

Salivary Duct Carcinoma With Rhabdoid Features-No or Aberrant Expression of E-cadherin and Genetic Changes in CDH1: Immunohistochemical and Genetic Analyses of 17 Cases.

Am J Surg Pathol 2021 Apr;45(4):439-449

Department of Pathology, Shizuoka General Hospital, Shizuoka City.

Salivary duct carcinoma is a relatively uncommon malignancy of the salivary glands; however, it frequently occurs as a carcinomatous component of carcinoma ex pleomorphic adenoma. We previously reported salivary duct carcinoma with rhabdoid features (SDCRF) as an extremely rare subtype of salivary duct carcinoma, and that it occurred as a salivary counterpart of pleomorphic lobular carcinoma of the breast (PLCB). We collected new cases of SDCRF for this study, in which we examined a total of 17 cases immunohistochemically and genetically. Read More

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