24 results match your criteria analysis hoct

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Morphological and Molecular Study of Hybrid Oncocytic/Chromophobe Tumor of the Kidney Associated with Sporadic Renal Oncocytosis and Chronic B-Cell Lymphocytic Leukemia: The Possible Contribution of Lymphoma to Renal Oncocytosis.

Pathobiology 2021 Apr 21:1-10. Epub 2021 Apr 21.

Pathology, University Hospital Virgen Macarena, University of Seville, Sevilla, Spain.

Hybrid oncocytic/chromophobe tumor (HOCT) of the kidney arising from a precursor oncocytosis not associated with the Birt-Hogg-Dubé (BHD) syndrome is an unusual and highly interesting neoplasm. Immunohistochemical and molecular findings suggest that HOCT is an entity distinct from both oncocytoma and chromophobe carcinoma. Although uncertainty persists regarding the factors predisposing to the development of HOCT, experimental findings suggest that it may arise due to the effect of toxins or in association with chronic kidney failure. Read More

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Assessment of Interaction of Human OCT 1-3 Proteins and Metformin Using Silico Analyses.

Acta Chim Slov 2020 Dec;67(4):1202-1215

Metformin, a drug frequently used by diabetic patients as the first-line treatment worldwide, is positively charged and is transported into the cell through human organic cation transporter (hOCT 1-3) proteins. We aimed to mimic the cellular uptake of metformin by hOCT1-3 with various bioinformatics methods and tools. 3D structure of OCT1-3 proteins was predicted by considering the structures and function of these proteins. Read More

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December 2020

Sporadic oncocytic tumors with features intermediate between oncocytoma and chromophobe renal cell carcinoma: comprehensive clinicopathological and genomic profiling.

Hum Pathol 2020 10 13;104:18-29. Epub 2020 Jul 13.

Department of Pathology, University of Washington, Seattle, WA, 98105, United States. Electronic address:

Morphology, clinical behavior, and genomic profiles of renal oncocytoma (RO) and its malignant counterpart chromophobe renal cell carcinoma (ChRCC) are distinctly different. However, there is a substantial group of sporadic oncocytic tumors with peculiar hybrid phenotypes as well as a perplexing degree of morphologic and immunohistochemical overlap between classic RO and ChRCC with eosinophilic cytoplasm. The aim of this study is to provide detailed characterization of these hybrid tumors. Read More

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October 2020

Characterization of -Iodobenzylguanidine (mIBG) Transport by Polyspecific Organic Cation Transporters: Implication for mIBG Therapy.

Mol Pharmacol 2020 08 2;98(2):109-119. Epub 2020 Jun 2.

Department of Pharmaceutics, University of Washington, Seattle, Washington

Radiolabeled -iodobenzylguanidine (mIBG) is an important radiopharmaceutical used in the diagnosis and treatment of neuroendocrine cancers. mIBG is known to enter tumor cells through the norepinephrine transporter. Whole-body scintigraphy has shown rapid mIBG elimination through the kidney and high accumulation in several normal tissues, but the underlying molecular mechanisms are unclear. Read More

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Repeatability of quantitative measurements of retinal layers with SD-OCT and agreement between vertical and horizontal scan protocols in healthy eyes.

PLoS One 2019 22;14(8):e0221466. Epub 2019 Aug 22.

Unit of Optometry, Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden.

Purpose: To evaluate the repeatability of the new spectral domain optical coherence tomography (HOCT-1F), and also to evaluate the agreement between vertical and horizontal scan protocols. In addition, we also evaluated the relation between the repeatability and age.

Methods: Three consecutive measurements of the inner limiting membrane-retinal pigment epithelium (ILM-RPE), inner limiting membrane-inner plexiform layer (ILM-IPL) from macular horizontal and vertical scans, and inner limiting membrane-retinal nerve fiber layer (ILM-RNFL) from optic disc horizontal scan. Read More

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Identification of the Tetraspanin CD9 as an Interaction Partner of Organic Cation Transporters 1 and 2.

SLAS Discov 2019 10 18;24(9):904-914. Epub 2019 Jul 18.

Medizinische Klinik D, Experimentelle Nephrologie, Westfälische Wilhelms-Universität, Münster, Germany.

Organic cation transporters (OCTs) are membrane proteins with relevant physiological (because they accept neurotransmitters as substrate) and pharmacological (because of their interaction with drugs) roles. The human OCTs hOCT1 (/hOCT1) and hOCT2 (/hOCT2) are highly expressed in hepatic (hOCT1) and in renal and neuronal tissue (hOCT2), suggesting a possible role in modulating neurotransmitter activity in the liver, kidney, and brain, and their clearance from the blood. Even though there are several data demonstrating that OCTs are regulated under various patho-physiological conditions, it remains largely unknown which proteins directly interact with OCTs and thereby influence their cellular processing, localization, and function. Read More

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October 2019

Hybrid oncocytic/chromophobe renal tumors are molecularly distinct from oncocytoma and chromophobe renal cell carcinoma.

Mod Pathol 2019 11 23;32(11):1698-1707. Epub 2019 Jun 23.

Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.

Hybrid oncocytic/chromophobe tumor (HOCT) of the kidney represents a poorly understood clinicopathologic entity with pathologic features that overlap between benign renal oncocytoma (RO) and malignant chromophobe renal cell carcinoma (ChRCC). Consequently, characterization of HOCT and its separation from the foregoing entities are clinically important. The aim of this study was to describe the pathologic and molecular features of HOCT and to compare them with those of RO and ChRCC. Read More

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November 2019

Hybrid oncocytic/chromophobe renal cell tumor: An integrated genetic and epigenetic characterization of a case.

Exp Mol Pathol 2018 12 18;105(3):352-356. Epub 2018 Oct 18.

Department of Pathology, Portuguese Oncology Institute of Porto, Portugal; Cancer Biology & Epigenetics Group, Research Center, Portuguese Oncology Institute of Porto, Portugal; Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Portugal. Electronic address:

Introduction: Hybrid oncocytic/chromophobe tumor (HOCT) is a renal cell neoplasm displaying overlapping cellular and architectural features of both renal oncocytoma (RO) and chromophobe renal cell carcinoma (chRCC). It has been described in the context of oncocytosis, Birt-Hogg-Dubé syndrome, and also sporadically. Thus far, HOCT immunohischemical profile and cytogenetic alterations have been reported, but not epigenetic alterations. Read More

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December 2018

Multiple drug transporters mediate the placental transport of sulpiride.

Arch Toxicol 2017 Dec 9;91(12):3873-3884. Epub 2017 Jun 9.

Laboratory of Pharmaceutical Analysis and Drug Metabolism, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.

Sulpiride is a typical antipsychotic drug for the treatment of schizophrenia, depression and other psychological disorders. It has been proven that a small amount of sulpiride could cross the human placenta using an ex vivo placental perfusion model. However, the placental transfer mechanism has not been elucidated. Read More

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December 2017

[Oncocytoma versus chromophobe renal cell carcinoma: Is there something in between?].

Pathologe 2016 Mar;37(2):153-8

Institut für Pathologie, Universitätsklinikum Bonn, Sigmund-Freud-Str. 25, 53127, Bonn, Deutschland.

The differentiation of oncocytic tumors of the kidneys is often difficult, particularly in renal biopsies. Differential diagnoses are chromophobe renal cell carcinoma (ChRCC), renal oncocytoma (RO), the oncocytic variant of papillary renal cell carcinoma (OPRCC), the eosinophilic variant of clear cell renal cell carcinoma (CCRCC) and hybrid oncocytic chromophobe tumors (HOCT). In difficult cases that cannot be resolved by morphology alone, immunohistochemistry is usually helpful. Read More

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Atenolol Renal Secretion Is Mediated by Human Organic Cation Transporter 2 and Multidrug and Toxin Extrusion Proteins.

Drug Metab Dispos 2015 Dec 15;43(12):1872-81. Epub 2015 Sep 15.

Department of Pharmaceutics, University of Washington, Seattle, Washington

Atenolol is a β-blocker widely used in the treatment of hypertension. Atenolol is cleared predominantly by the kidney by both glomerular filtration and active secretion, but the molecular mechanisms involved in its renal secretion are unclear. Using a panel of human embryonic kidney cell lines stably expressing human organic cation transporter (hOCT) 1-3, human organic anion transporter (hOAT) 1, hOAT3, human multidrug and toxin extrusion protein (hMATE) 1, and hMATE2-K, we found that atenolol interacted with both organic cation and anion transporters. Read More

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December 2015

Immunohistochemical characterization of renal tumors in patients with Birt-Hogg-Dubé syndrome.

Pathol Int 2015 Mar 19;65(3):126-32. Epub 2015 Jan 19.

Department of Molecular Pathology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.

Birt-Hogg-Dubé syndrome (BHD) is an autosomal dominant disorder associated with a germline mutation of folliculin (FLCN). The affected families are at a high risk for developing multiple renal cell carcinomas (RCC). Little is known about the immunostaining patterns of mutant FLCN-associated RCCs. Read More

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Renal cell carcinoma in tuberous sclerosis complex.

Am J Surg Pathol 2014 Jul;38(7):895-909

Departments of *Pathology **Urology ††Carol and James Herscot Center for Tuberous Sclerosis Complex, Massachusetts General Hospital ‡‡Division of Translational Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston ∥Department of Pathology, University of Massachusetts Medical School, Worcester, MA †Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou §Department of Pathology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, PR China ‡Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN #Department of Medicine, Division of Genomic Medicine, Institute for Human Genetics, University of California, San Francisco, CA ¶Department of Neurology and Epileptology, The Children's Memorial Health Institute, Warsaw, Poland.

Renal cell carcinoma (RCC) occurs in 2% to 4% of patients with tuberous sclerosis complex (TSC). Previous reports have noted a variety of histologic appearances in these cancers, but the full spectrum of morphologic and molecular features has not been fully elucidated. We encountered 46 renal epithelial neoplasms from 19 TSC patients and analyzed their clinical, pathologic, and molecular features, enabling separation of these 46 tumors into 3 groups. Read More

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Inhibition of organic cation transporter 2 and 3 may be involved in the mechanism of the antidepressant-like action of berberine.

Prog Neuropsychopharmacol Biol Psychiatry 2014 Mar 15;49:1-6. Epub 2013 Nov 15.

Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, PR China. Electronic address:

Organic cation transporter 2 (OCT2) and 3 (OCT3) are low-affinity, high-capacity transporters (uptake-2) expressed in the central nervous system (CNS) and other major organs. Proven to be essential components in the CNS functions, OCT2 and OCT3 are suggested as potential targets of antidepressant therapeutics recently. Berberine, an active constituent derived from many medicinal plants, such as Coptis chinensis, has been reported to possess antidepressant-like action in the tail suspension test and forced swim test with elevated serotonin/norepinephrine/dopamine (5-HT/NE/DA) level in mouse brain; however the mechanism has not been elucidated. Read More

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Hybrid oncocytic/chromophobe renal cell tumours do not display genomic features of chromophobe renal cell carcinomas.

Virchows Arch 2013 Jun 25;462(6):633-8. Epub 2013 May 25.

Department of Pathology, Cochin Hospital, Assistance Publique Hôpitaux de Paris, Paris Descartes University, Paris, France.

Hybrid oncocytic/chromophobe tumours (HOCT) are renal tumours recently described displaying histological features of both renal oncocytoma (RO) and chromophobe renal cell carcinoma (ChRCC), raising the question of their precise signification in the RO/ChRCC group. This study aimed to describe clinicopathological features of so called HOCT and to characterise their genomic profile. Five hundred and eighty-three tumours belonging to the ChRCC/RO group were retrospectively reviewed. Read More

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A review of the clinical pharmacokinetics and pharmacodynamics of varenicline for smoking cessation.

Clin Pharmacokinet 2010 Dec;49(12):799-816

Clinical Pharmacology, Primary Care Unit, Pfizer Inc., New London, Connecticut 06320, USA.

Varenicline tartrate (Chantix®/Champix®) is a selective partial agonist of the α(4)β(2) nicotinic acetylcholine receptor and is approved as an aid to smoking cessation. The usual oral dosage in adults is 1 mg twice daily for 12 weeks, with an initial titration week. Several clinical pharmacology studies have characterized the pharmacokinetics of varenicline in adult smokers aged 18-55 years, elderly smokers and nonsmokers aged ≥ 65 years, adolescent smokers aged 12-17 years and subjects with impaired renal function. Read More

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December 2010

Organic cation transporter-mediated renal secretion of ipratropium and tiotropium in rats and humans.

Drug Metab Dispos 2011 Jan 20;39(1):117-22. Epub 2010 Oct 20.

Department of Membrane Transport and Biopharmaceutics, Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical, and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, Japan.

Ipratropium bromide (ipratropium) and tiotropium bromide (tiotropium), anticholinergic agents with bronchodilating properties, are used to treat patients with chronic obstructive pulmonary disease. Because they are actively secreted into urine, the interaction of these agents with organic cation transporters (OCTs/Octs) was examined in rat kidney slices and in cultured cells expressing rat Oct (rOct) or human OCT (hOCT). Uptake of radiolabeled ipratropium in rat kidney slices was significantly inhibited by OCT/Oct substrates including cimetidine, imipramine, and quinidine, but not by organic anion transporter substrates (e. Read More

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January 2011

Imatinib dose escalation in two patients with chronic myeloid leukemia, with low trough imatinib plasma levels measured at various intervals from the beginning of therapy and with suboptimal treatment response, leads to the achievement of higher plasma levels and major molecular response.

Int J Hematol 2010 Jun 1;91(5):897-902. Epub 2010 May 1.

Department of Hemato-Oncology, Faculty of Medicine and Dentistry, University Palacky in Olomouc, Faculty Hospital Olomouc, IP Pavlova 6, 775 20, Olomouc, Czech Republic.

Despite the prognostic value of trough imatinib plasma levels (IPL) identified in some studies, no recommendations for the use of IPL results in routine management of CML patients have been issued. We report two patients in whom daily imatinib dose was increased from 400 to 600 or 800 mg because of low IPL found at various intervals from the beginning of treatment (7 measurements; mean IPL values = 616.33 and 764. Read More

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Sporadic hybrid oncocytic/chromophobe tumor of the kidney: a clinicopathologic, histomorphologic, immunohistochemical, ultrastructural, and molecular cytogenetic study of 14 cases.

Virchows Arch 2010 Apr 19;456(4):355-65. Epub 2010 Mar 19.

Department of Pathology, National University Hospital System, Singapore, Singapore.

Hybrid oncocytic/chromophobe tumors (HOCT) of the kidney have been described in patients with Birt-Hogg-Dubé syndrome (BHD) and in association with renal oncocytosis without BHD. HOCT in patients without evidence of BHD or renal oncocytosis is exceedingly rare, and these cases have been poorly characterized. We have identified and studied 14 cases of HOCT from previously diagnosed renal oncocytomas (398 cases) and chromophobe renal cell carcinomas (351 cases) without evidence of BHD or renal oncocytosis. Read More

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Comparison of different modes in optical coherence tomography and ultrasound biomicroscopy in anterior chamber angle assessment.

J Glaucoma 2009 Aug;18(6):472-8

Department of Ophthalmology, University of California, San Francisco, CA, USA.

Purpose: To compare anterior chamber angle width measurements by low and high-resolution anterior segment optical coherence tomography (AS-OCT) and ultrasound biomicroscopy (UBM).

Patients And Methods: Thirty-six eyes of 32 patients with gonioscopically narrow angles from a university-based glaucoma clinic were recruited in the study. All subjects received AS-OCT {in "Anterior Segment Single" mode [low-resolution optical coherence tomography (LOCT)] and "High-resolution Cornea" mode [high-resolution optical coherence tomography (HOCT)]} and UBM examination in 1 or both eyes. Read More

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Identification and functional characterization of genetic variants of human organic cation transporters in a Korean population.

Drug Metab Dispos 2007 Apr 12;35(4):667-75. Epub 2007 Jan 12.

Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine, 633-165 Gaegum 2-Dong, Jin-Gu, Busan 614-735, Korea.

Genetic variants of three human organic cation transporter genes (hOCTs) were extensively explored in a Korean population. The functional changes of hOCT2 variants were evaluated in vitro, and those genetic polymorphisms of hOCTs were compared among different ethnic populations. From direct DNA sequencing, 7 of 13 coding variants were nonsynonymous single-nucleotide polymorphisms (SNPs), including four variants from hOCT1 (F160L, P283L, P341L, and M408V) and three from hOCT2 (T199I, T201M, and A270S), whereas 6 were synonymous SNPs. Read More

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Drug specificity and intestinal membrane localization of human organic cation transporters (OCT).

Biochem Pharmacol 2005 Dec 2;70(12):1851-60. Epub 2005 Nov 2.

Membrane Transport Group, Biozentrum of the Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, D-06120 Halle, Germany.

This study was performed to investigate which human organic cation transporter, hOCT1, hOCT2 or hOCT3, participates with regard to cation specificity and membrane localization in the intestinal absorption of orally available cationic drugs. Inhibition of N-[methyl-3H]4-phenylpyridinium ([3H]MPP+) uptake by various compounds into Caco-2 cells and into cells (HEK-293 or CHO) that were stably transfected with hOCT1, hOCT2 or hOCT3 was compared. The uptake of [3H]MPP+ into Caco-2 cells was inhibited by atropine, butylscopolamine, clonidine, diphenhydramine, etilefrine, quinine and ranitidine with IC50 values between 6 microM and 4 mM. Read More

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December 2005

Expression and pharmacological profile of the human organic cation transporters hOCT1, hOCT2 and hOCT3.

Br J Pharmacol 2002 Jul;136(6):829-36

Institute of Pharmacology and Toxicology, University of Bonn, Reuterstr. 2 b, D-53113 Bonn, Germany.

1. Organic cation transporters (OCTs) are involved in the elimination of monoamines and cationic xenobiotics. To examine whether some cell lines express several different OCTs, we investigated seven human cell lines for the mRNA expression pattern of the human (h) transporters hOCT1, hOCT2 and hOCT3. Read More

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Variables that discriminate bulimic from nonbulimic adolescent females.

G Post J H Crowther

J Youth Adolesc 1985 Apr;14(2):85-98

Kent State University, USA.

The present study identified variables that discriminated bulimic (N=71) from nonbulimic (N=71) adolescent females within a high school setting. A discriminant function analysis identified six variables that significantly differentiated these two groups. These variables included disturbed eating attitudes, negative perceptions of weight and body image, depressive symptomatology, and alcohol use. Read More

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