22,273 results match your criteria analysis charged


Effect of pH adjustment and ratio of oppositely charged polymers on the mechanistic performance and sustained release of volatile perfume in interpolyelectrolyte complex microcapsules.

Int J Pharm 2021 May 4:120672. Epub 2021 May 4.

College of Pharmacy, Ajou University, Suwon 16499, Republic of Korea. Electronic address:

In this study, volatile perfume was encapsulated in microcapsules (MCs) via interpolyelectrolyte complexes (IPECs) of oppositely charged polymers, with high encapsulation efficiency, to be delivered in a sustained manner. Positively charged chitosan (CTS) and negatively charged Eudragit® S100 (ES100) were used as eco-friendly biopolymers. Limonene (LMN) was selected as the model perfume. Read More

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Test of Lepton-Flavor Universality in B→K^{*}ℓ^{+}ℓ^{-} Decays at Belle.

Phys Rev Lett 2021 Apr;126(16):161801

INFN-Sezione di Napoli, 80126 Napoli.

We present a measurement of R_{K^{*}}, the branching fraction ratio B(B→K^{*}μ^{+}μ^{-})/B(B→K^{*}e^{+}e^{-}), for both charged and neutral B mesons. The ratio for the charged case R_{K^{*+}} is the first measurement ever performed. In addition, we report absolute branching fractions for the individual modes in bins of the squared dilepton invariant mass q^{2}. Read More

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Development of acidic phospholipid containing immobilized artificial membrane column to predict drug-induced phospholipidosis potency.

J Chromatogr A 2021 Apr 15;1647:462147. Epub 2021 Apr 15.

Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China.

Drug-induced phospholipidosis (DIPLD) represents a big concern for both regulatory authorities and pharmaceutical companies in drug discovery. Many researches pointed out that the negatively charged intralysosomal lipids play an important role in the formation of DIPLD. To better mimic this negatively charged lipid surface, a novel immobilized artificial membrane (IAM) column was prepared via in situ copolymerization of 12-methacryloyl n-dodecylphosphocholine (MDPC) and 12-methacryloyl n-dodecylphosphoric acid (MDPA). Read More

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The macroeconomics of abortion: A scoping review and analysis of the costs and outcomes.

PLoS One 2021 6;16(5):e0250692. Epub 2021 May 6.

Ipas, Chapel Hill, North Carolina, United States of America.

Background: Although abortion is a common gynecological procedure around the globe, we lack synthesis of the known macroeconomic costs and outcomes of abortion care and abortion policies. This scoping review synthesizes the literature on the impact of abortion-related care and abortion policies on economic outcomes at the macroeconomic level (that is, for societies and nation states).

Methods And Findings: Searches were conducted in eight electronic databases. Read More

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Structural studies of hemoglobin from two flightless birds, ostrich and turkey: insights into their differing oxygen-binding properties.

Acta Crystallogr D Struct Biol 2021 May 26;77(Pt 5):690-702. Epub 2021 Apr 26.

Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India.

Crystal structures of hemoglobin (Hb) from two flightless birds, ostrich (Struthio camelus) and turkey (Meleagris gallopova), were determined. The ostrich Hb structure was solved to a resolution of 2.22 Å, whereas two forms of turkey Hb were solved to resolutions of 1. Read More

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Effect of Fluorescent Labels on DNA Affinity for Gold Nanoparticles.

Nanomaterials (Basel) 2021 Apr 29;11(5). Epub 2021 Apr 29.

Institute of Chemical Biology and Fundamental Medicine, SB RAS, Lavrentiev ave. 8, 630090 Novosibirsk, Russia.

Fluorophore (FD) labeling is widely used for detection and quantification of various compounds bound to nanocarriers. The systems, composed of gold nanoparticles (GNPs) and oligonucleotides (ONs) labeled with FDs, have wide applications. Our work was aimed at a systemic study of how FD structure (in composition of ON-FDs) influenced the efficiency of their non-covalent associates' formation with GNPs (ON-FD/GNPs). Read More

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Molecular Mechanism of Small-Molecule Inhibitors in Blocking the PD-1/PD-L1 Pathway through PD-L1 Dimerization.

Int J Mol Sci 2021 Apr 30;22(9). Epub 2021 Apr 30.

Key Laboratory for Bio-Based Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510630, China.

Programmed cell death-1 (PD-1), which is a molecule involved in the inhibitory signal in the immune system and is important due to blocking of the interactions between PD-1 and programmed cell death ligand-1 (PD-L1), has emerged as a promising immunotherapy for treating cancer. In this work, molecular dynamics simulations were performed on complex systems consisting of the PD-L1 dimer with (S)-BMS-200, (R)-BMS-200 and (MOD)-BMS-200 (i.e. Read More

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: Test case of a screen confession.

Authors:
Dana Amir

Int J Psychoanal 2020 Jun 6;101(3):597-610. Epub 2019 Dec 6.

Department of Counseling and Human Development, University of Haifa, Haifa, Israel.

An analysis of the transcripts of interviews by Claude Lanzmann with Benjamin Murmelstein in the documentary , dealing with Jewish collaboration with the Nazis in World War II, reveals the speaker producing a "screen confession," an ambiguous text that dissociates between explicit and implicit contents, and while presenting an eloquent and coherent narrative actually rewrites emotional, maybe even factual, history. Unlike Freud's "screen memory," which refers to the way in which a marginal memory covers another emotionally charged one which cannot be remembered, the notion of screen confession refers not to memory itself but to how it is construed in language. Omitted from this kind of confession are not the concrete facts, but their meaning. Read More

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Oscillatory shear-induced bcc-fcc martensitic transformation in a colloidal suspension with long-range repulsive interactions.

J Chem Phys 2021 Apr;154(16):164903

Soft Condensed Matter, Debye Institute for Nanomaterials Science, Department of Physics, Utrecht University, Princetonplein 1, 3584 CC Utrecht, The Netherlands.

We perform non-equilibrium Brownian dynamics simulations to investigate the out-of-equilibrium phase behavior of a suspension of charged colloids under external oscillatory shear. We independently vary the frequency f and the maximum strain amplitude γ of the oscillations and map out an out-of-equilibrium phase diagram in the f-γ plane. Similar to what has been observed in earlier studies on colloidal hard spheres, we find the formation of a twinned face-centered-cubic phase in a specific range of γ, which displays a martensitic transition to a body-centered-cubic crystal within half of the oscillation cycle. Read More

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Linear Free Energy Relationships for Enzymatic Reactions: Fresh Insight from a Venerable Probe.

Acc Chem Res 2021 May 3. Epub 2021 May 3.

Department of Chemistry, University at Buffalo, SUNY, Buffalo, New York 14260-3000, United States.

ConspectusLinear free energy relationships (LFERs) for substituent effects on reactions that proceed through similar transition states provide insight into transition state structures. A classical approach to the analysis of LFERs showed that differences in the slopes of Brønsted correlations for addition of substituted alkyl alcohols to ring-substituted 1-phenylethyl carbocations and to the β-galactopyranosyl carbocation intermediate of reactions catalyzed by β-galactosidase provide evidence that the enzyme catalyst modifies the curvature of the energy surface at the saddle point for the transition state for nucleophile addition. We have worked to generalize the use of LFERs in the determination of enzyme mechanisms. Read More

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Analysis of electrostatic coupling throughout the laboratory evolution of a designed retroaldolase.

Protein Sci 2021 May 2. Epub 2021 May 2.

Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA.

The roles of local interactions in the laboratory evolution of a highly active, computationally designed retroaldolase (RA) are examined. Partial Order Optimum Likelihood (POOL) is used to identify catalytically important amino acid interactions in several RA95 enzyme variants. The series RA95. Read More

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Analysis of Binding Modes of Antigen-Antibody Complexes by Molecular Mechanics Calculation.

J Chem Inf Model 2021 May 3. Epub 2021 May 3.

Graduate School of Pharmaceutical Sciences, Chiba UniversityRINGGOLD, Inohana 1-8-1, Chuo-ku, Chiba 260-8675, Japan.

Antibodies are one of the most important protein molecules in biopharmaceutics. Due to the recent advance in technology for producing monoclonal antibodies, many structural data are available on the antigen-antibody complexes. To characterize the molecular interaction in antigen-antibody recognition, we computationally analyzed 500 complex structures by molecular mechanics calculations. Read More

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Amination of cotton fiber using polyethyleneimine and its application as an adsorbent to directly remove a harmful cyanobacterial species, Microcystis aeruginosa, from an aqueous medium.

Environ Res 2021 Apr 29;197:111235. Epub 2021 Apr 29.

Division of Environmental Science & Ecological Engineering, Korea University, Seoul, 02841, Republic of Korea. Electronic address:

In the present study, we applied an adsorption-based strategy for the removal of a harmful cyanobacterial species, Microcystis aeruginosa, using cotton fiber. Considering the negatively charged surface properties of M. aeruginosa cells in aqueous phases, aminated cotton fibers were prepared through polyethyleneimine (PEI) modification on the pristine cotton fibers. Read More

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Simultaneous determination of six main components in Bushen Huoxue prescription by HPLC-CAD.

J Pharm Biomed Anal 2021 Apr 23;201:114087. Epub 2021 Apr 23.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, No. 1166 Liutai Avenue, Wenjiang District, Chengdu, Sichuan, 611137, China. Electronic address:

Background: The Bushen Huoxue prescription is a traditional Chinese medicine formula treating diabetic retinopathy, which was developed by our research group. Catalpol, puerarin, salvianolic acid B, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd are six of main effective components, which could be partly representative of this prescription. The corona charged aerosol detector (corona CAD) is one kind of universal detectors equipped with the high performance liquid chromatography (HPLC). Read More

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A study of Kollicoat® MAE100P film's Structure and Properties.

Int J Pharm 2021 Apr 28:120622. Epub 2021 Apr 28.

College of Pharmacy, University of Iowa, Iowa City, Iowa, USA.

Generally, an organic-solvent-based film is denser and tougher than a corresponding aqueous-dispersion-based film. However, Kollicoat® MAE100P films prepared from aqueous dispersions had greater tensile strengths compared to the films cast from organic solutions. It was proposed that MAE100P polymer particles in aqueous media had a core-shell structure with a hydrophilic shell and a hydrophobic core. Read More

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PN junction and band to band tunneling in carbon nanotube transistors at room temperature.

Nanotechnology 2021 Apr 30. Epub 2021 Apr 30.

Electrical Engineering, Technion Israel Institute of Technology Faculty of Electrical Engineering, Technion city, Haifa, 32000, ISRAEL.

We demonstrate band to band tunneling (BTBT) in a carbon-nanotube (CNT) field effect transistor. We employ local electrostatic doping assisted by charged traps within the oxide to produce an intramolecular PN junction along the CNT. These characteristics apply for both metallic (m-CNTs) and semiconducting (SC-CNTs) CNTs. Read More

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Early career midwives' perception of their teamwork skills following a specifically designed, whole-of-degree educational strategy utilising groupwork assessments.

Midwifery 2021 Apr 14;99:102997. Epub 2021 Apr 14.

The University of Sydney, School of Medicine, University Centre for Rural Health, PO Box 3074 Lismore NSW Australia 2480. Electronic address:

Objective: The aim of this study was to investigate whether a specifically designed whole-ofdegree strategy utilising groupwork assessments was effective in facilitating the development of early career midwives' teamwork skills.

Design And Methods: A qualitative study using in-depth, semi-structured interviews was undertaken with early career midwives who had graduated within the previous two years. This study is the final cycle of a larger participatory action research project. Read More

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Identification and Amino Acid Analysis of Allergenic Epitopes of a Novel Allergen Paramyosin (Rap v 2) from .

J Agric Food Chem 2021 Apr 30. Epub 2021 Apr 30.

College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province 266003, P.R. China.

Besides tropomyosin (TM) that is widely recognized as a major allergen in molluscs, a 99-kDa novel allergen (Rap v 2) was recently found in the sea snail and identified as paramyosin (PM). However, the allergenic epitopes of PM in any molluscs have not been identified yet. In the present study, seven allergenic epitopes of Rap v 2 were identified by immunoinformatics tools, dot-blot inhibition assay, and basophil degranulation assay. Read More

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Spectroscopic characterization of a thermodynamically stable doubly charged diatomic molecule: MgAr.

Phys Chem Chem Phys 2021 Apr 30. Epub 2021 Apr 30.

Laboratory of Physical Chemistry, ETH Zurich, CH-8093 Zurich, Switzerland.

Although numerous doubly positively charged diatomic molecules (diatomic dications) are known from investigations using mass spectrometry and ab initio quantum chemistry, only three of them, NO2+, N22+ and DCl2+, have been studied using rotationally resolved optical spectroscopy and only about a dozen by vibrationally resolved double-ionization methods. So far, no thermodynamically stable diatomic dication has been characterized spectroscopically, primarily because of experimental difficulties associated with their synthesis in sufficient densities in the gas phase. Indeed, such molecules typically involve, as constituents, rare-gas, halogen, chalcogen, and metal atoms. Read More

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Quartz Crystal Microbalance with Dissipation Monitoring of Dynamic Viscoelastic Changes of Tobacco BY-2 Cells under Different Osmotic Conditions.

Biosensors (Basel) 2021 Apr 27;11(5). Epub 2021 Apr 27.

College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.

The plant cell mechanics, including turgor pressure and wall mechanical properties, not only determine the growth of plant cells, but also reflect the functional and structural changes of plant cells under biotic and abiotic stresses. However, there are currently no appropriate techniques allowing to monitor the complex mechanical properties of living plant cells non-invasively and continuously. In this work, quartz crystal microbalance with dissipation (QCM-D) monitoring technique with overtones (3-9) was used for the dynamic monitoring of adhesions of living tobacco BY-2 cells onto positively charged N,N-dimethyl-N-propenyl-2-propen-1-aminiumchloride homopolymer (PDADMAC)/SiO QCM crystals under different concentrations of mannitol (C) and the subsequent effects of osmotic stresses. Read More

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Visual and Optical Absorbance Detection of Melamine in Milk by Melamine-Induced Aggregation of Gold Nanoparticles.

Nanomaterials (Basel) 2021 Apr 28;11(5). Epub 2021 Apr 28.

Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut 22200, Terengganu, Malaysia.

The present study reported a facile method for the determination of melamine in milk powder products based on the aggregation of reactant-free 5 nm gold nanoparticles (AuNPs). The strong electrostatic attraction between the positively charged exocyclic amine groups present in the melamine molecule and the negatively charged ions bound to the AuNPs induced aggregation of the AuNPs, resulting in visible color changes that could be seen with the naked eye and monitored by ultraviolet-visible (UV-Vis) absorbance spectra. The method shows high sensitivity with detection limits of 1 × 10 M for visual detection and 1 × 10 M for UV-Vis analysis, which is far below the safety limit of melamine ingestion in infant formula (1 ppm = 7. Read More

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Conserved Conformational Hierarchy across Functionally Divergent Glycosyltransferases of the GT-B Structural Superfamily as Determined from Microsecond Molecular Dynamics.

Int J Mol Sci 2021 Apr 28;22(9). Epub 2021 Apr 28.

Bioinformatics and Computational Biology Program, University of Minnesota, Minneapolis, MN 55455, USA.

It has long been understood that some proteins undergo conformational transitions en route to the Michaelis Complex to allow chemistry. Examination of crystal structures of glycosyltransferase enzymes in the GT-B structural class reveals that the presence of ligand in the active site triggers an open-to-closed conformation transition, necessary for their catalytic functions. Herein, we describe microsecond molecular dynamics simulations of two distantly related glycosyltransferases that are part of the GT-B structural superfamily, HepI and GtfA. Read More

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Analysis of Ionic Domains on a Proton Exchange Membrane Using a Numerical Approximation Model Based on Electrostatic Force Microscopy.

Polymers (Basel) 2021 Apr 13;13(8). Epub 2021 Apr 13.

Tabula Rasa College, Keimyung University, Daegu 42601, Korea.

Understanding the ionic channel network of proton exchange membranes that dictate fuel cell performance is crucial when developing proton exchange membrane fuel cells. However, it is difficult to characterize this network because of the complicated nanostructure and structure changes that depend on water uptake. Electrostatic force microscopy (EFM) can map surface charge distribution with nano-spatial resolution by measuring the electrostatic force between a vibrating conductive tip and a charged surface under an applied voltage. Read More

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Evaluation of Electrochemical Stability of Sulfonated Anthraquinone-Based Acidic Electrolyte for Redox Flow Battery Application.

Molecules 2021 Apr 24;26(9). Epub 2021 Apr 24.

Department of Chemical Engineering, University of Chemistry and Technology, Technická 5, Praha 6, 166 28 Prague, Czech Republic.

Despite intense research in the field of aqueous organic redox flow batteries, low molecular stability of electroactive compounds limits further commercialization. Additionally, currently used methods typically cannot differentiate between individual capacity fade mechanisms, such as degradation of electroactive compound and its cross-over through the membrane. We present a more complex method for in situ evaluation of (electro)chemical stability of electrolytes using a flow electrolyser and a double half-cell including permeation measurements of electrolyte cross-over through a membrane by a UV-VIS spectrometer. Read More

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The Role of Polymeric Coatings for a Safe-by-Design Development of Biomedical Gold Nanoparticles Assessed in Zebrafish Embryo.

Nanomaterials (Basel) 2021 Apr 14;11(4). Epub 2021 Apr 14.

Department of Biotechnology and Biosciences, University of Milano-Bicocca, 20126 Milan, Italy.

In the biomedical field, gold nanoparticles (GNPs) have attracted the attention of the scientific community thanks to their high potential in both diagnostic and therapeutic applications. The extensive use of GNPs led researchers to investigate their toxicity, identifying stability, size, shape, and surface charge as key properties determining their impact on biological systems, with possible strategies defined to reduce it according to a Safe-by-Design (SbD) approach. The purpose of the present work was to analyze the toxicity of GNPs of various sizes and with different coating polymers on the developing vertebrate model, zebrafish. Read More

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Association of Bovine Leukemia Virus-Induced Lymphoma with BoLA-DRB3 Polymorphisms at DNA, Amino Acid, and Binding Pocket Property Levels.

Pathogens 2021 Apr 6;10(4). Epub 2021 Apr 6.

Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Bovine leukemia virus (BLV) causes enzootic bovine leucosis, a malignant B-cell lymphoma in cattle. The DNA sequence polymorphisms of bovine leukocyte antigen (BoLA) have exhibited a correlation with BLV-induced lymphoma in Holstein cows. However, the association may vary between different cattle breeds. Read More

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Enhanced Structural Stability and Electrochemical Performance of LiNiCoMnO Cathode Materials by Ga Doping.

Materials (Basel) 2021 Apr 7;14(8). Epub 2021 Apr 7.

Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China.

Structural instability during cycling is an important factor affecting the electrochemical performance of nickel-rich ternary cathode materials for Li-ion batteries. In this work, enhanced structural stability and electrochemical performance of LiNiCoMnO cathode materials are achieved by Ga doping. Compared with the pristine electrode, Li[NiCoMn]GaO electrode exhibits remarkably improved electrochemical performance and thermal safety. Read More

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Experimental Verification of the Balance between Elastic Pressure and Ionic Osmotic Pressure of Highly Swollen Charged Gels.

Gels 2021 Apr 1;7(2). Epub 2021 Apr 1.

Faculty of Advanced Life Science, Hokkaido University, Sapporo 001-0021, Japan.

The equilibrium swelling degree of a highly swollen charged gel has been thought to be determined by the balance between its elastic pressure and ionic osmotic pressure. However, the full experimental verification of this balance has not previously been conducted. In this study, we verified the balance between the elastic pressure and ionic osmotic pressure of charged gels using purely experimental methods. Read More

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Critical Impact of Nitrogen Vacancies in Nonradical Carbocatalysis on Nitrogen-Doped Graphitic Biochar.

Environ Sci Technol 2021 Apr 29. Epub 2021 Apr 29.

Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon 852, Hong Kong, China.

Nitrogen-doped graphitic biochar (NBC) has boosted the development of nonradical peroxymonosulfate (PMS) activation in environmental remediation. However, the specific role of nitrogen species played in NBC-based nonradical carbocatalysis remains vaguely interpreted. To pinpoint the critical nitrogen speciation, a sophisticated thermo-mechanochemical manipulation was exploited to prepare a series of NBCs with similar dimensional structures and oxygen levels but different nitrogen species (. Read More

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Pragmatic mAb lead molecule engineering from a developability perspective.

Biotechnol Bioeng 2021 Apr 29. Epub 2021 Apr 29.

Kymab Ltd., Bennet Building, Babraham Research Campus, Cambridge, CB22 3AT.

As the number of antibody drugs being approved and marketed increases, our knowledge of what makes potential drug candidates a successful product has increased tremendously. One of the critical parameters that has become clear in the field is the importance of mAb 'developability'. Efforts are being increasingly focused on simultaneously selecting molecules that exhibit both desirable biological potencies and manufacturability attributes. Read More

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