21,637 results match your criteria amplified primers


Inheritance of seedlessness and the molecular characterization of the INO gene in Annonaceae.

Braz J Biol 2021 21;83:e246455. Epub 2021 Jun 21.

Universidade Federal de Minas Gerais - UFMG, Instituto de Ciências Agrárias, Montes Claros, MG, Brasil.

The inheritance of the seedless fruit characteristic of Annona squamosa has not yet been explained. Molecular techniques may aid breeding programs, mainly in the assisted selection of the target gene. The INO gene may be related to seed development in these fruits. Read More

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First report of causing spring dead spot of hybrid bermudagrass in China.

Plant Dis 2021 Jun 23. Epub 2021 Jun 23.

Nanjing Agricultural University, 70578, Agro-grassland Science, Nanjing, Jiangsu, China;

Hybrid bermudagrass (Cynodon dactylon×C. transvaalensis) is widely used as turf in transition zone of China. Spring dead spot (SDS) is one of the most damaging diseases of hybrid bermudagrass. Read More

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First report of Neopestalotiopsis protearum causing seed rot on Camellia oleifera in China.

Plant Dis 2021 Jun 22. Epub 2021 Jun 22.

Central South University of Forestry and Technology, 12571, Changsha, Hunan, China;

Camellia oleifera, an evergreen small tree or shrub with high medicinal and ecological values, is mainly distributed in subtropical regions of China. Camellia oil obtained from Camellia oleifera seeds is rich in unsaturated fatty acids and unique flavors, and has become a rising high-quality edible vegetable oil in south of China (Zhuang 2008). The tea-oil tree Camellia oleifera plays important economic and ecological roles in Hunan province. Read More

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First report of Fusarium sacchari causing sugarcane wilt in Bangladesh.

Plant Dis 2021 Jun 22. Epub 2021 Jun 22.

Bangabandhu Sheikh Mujibur Rahman Agricultural University, 198780, Department of Biotechnology, Salna, Gazipur, Bangladesh;

Wilt is one of the destructive fungal diseases of sugarcane (Saccharum officinarum L.) affecting sugarcane production in many sugarcane growing regions including Bangladesh. In July 2018, sugarcane plants showing wilting symptom including withered, dried leaves and shriveling stalks with brown to pinkish discoloration in internal tissues (Viswanathan, 2013) were collected from sugarcane field from Gazipur district (23°54'41″ N, 90°23'20″ E) of Bangladesh. Read More

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Occurrence of Powdery Mildew Caused by Erysiphe buhrii on Dianthus chinensis in Inner Mongolia, China.

Plant Dis 2021 Jun 22. Epub 2021 Jun 22.

Inner Mongolia Agricultural University, 117454, College of Horticulture and Plant Protection, Hohhot, Inner Mongolia, China;

Dianthus chinensis is widely cultivated for ornamental and medicinal use in China (Guo et al. 2017). The plant has been used in traditional Chinese medicine for the treatment of urinary problems such as strangury and diuresis (Han et al. Read More

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First Report of Passion Fruit Anthracnose Caused by Colletotrichum constrictum.

Plant Dis 2021 Jun 22. Epub 2021 Jun 22.

Chinese Academy of Agricultural Sciences, Research Institute of Pomology, XINGHAINANJIE 98#, XINGCHENG, XINGCHENG, LIAONING, China, 125100.

Passion fruit (Passiflora edulis) is widely cultivated in tropic and subtropic regions. Because of its unique and intense flavour and high acidity, passion fruit juice concentrate is used in making delectable sauces, desserts, candy, ice cream, sherbet, or blending with other fruit juices. Anthracnose of passion fruit is favored by frequent rainfall and average temperatures above 27°C. Read More

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First report of Anthracnose on Peach Fruit Caused by Colletotrichum siamense in Uruguay.

Plant Dis 2021 Jun 18. Epub 2021 Jun 18.

Universidad de la República, Departamento de Protección Vegetal, Garzón 780, Montevideo, Uruguay, 12900;

Peach (Prunus persica L.) is an economically important deciduous fruit crop in Uruguay. Anthracnose caused by species of the genus Colletotrichum is one of the major diseases in peach production, originating significant yield losses in United States (Hu et al. Read More

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First Report of Tomato Powdery Mildew Caused by in Taiwan.

Plant Dis 2021 Jun 18. Epub 2021 Jun 18.

Taiwan Agricultural Research Institute, 56091, Plant Pathology, Taichung, Taiwan;

Tomato ( L.) is one of the common and important economic crops in Taiwan. In July 2018, tomato leaves with unusual yellowing and lesions were observed in Taichung, Changhua, Nantou, and Yunlin counties in Taiwan. Read More

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First report of Fusarium proliferatum on date palm (Phoenix dactylifera L.) in Jordan.

Plant Dis 2021 Jun 18. Epub 2021 Jun 18.

The University of Jordan, Amman, Jordan;

Date palm (Phoenix dactylifera L.) is one of the world's oldest cultivated fruit crops. In Jordan, date palm farming started in the 1990s. Read More

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Pathogen identification of root-knot nematode disease in Yulong, China.

J Nematol 2021 12;53. Epub 2021 Jun 12.

School of Landscape and Horticulture, Southwest Forestry University, Yunnan, Kunming 650233, China.

In September 2020, samples of galled roots with rhizosphere soil were collected from declining in Yulong County, China. The pathogenic nematodes were identified by observing morphological characteristics of females, second-stage juveniles and perineal pattern, sequence alignments, and specific amplification of sequence characterized amplified region (SCAR). The results showed that the perineal pattern of this nematode was round or oval, the dorsal arch was moderately high or low, one side or both of the lateral field extended to form a wing shape, the tail region had punctations, and the morphological characteristics and morphometric values of second-stage juveniles and females were similar to those of . Read More

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First Report of Leaf Spot Caused by Ramularia sphaeroidea on Vicia villosa var. glabrescens in China.

Plant Dis 2021 Jun 15. Epub 2021 Jun 15.

Lanzhou University, College of Pastoral Agriculture Science and Technology, No. 768, Jiayuguan West Road, Lanzhou, Gansu, China, 730020.

Hairy vetch is widely grown in southwestern China for green manure and forage. In December 2019, a leaf disease occurred on 80% plants of in an eight-hectare field in Qujing(N 25°28'12″, E 103°36'22″), Yunnan Province, China. The disease leaves had irregular, brown to dark brown leaf spots with white mold. Read More

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Microscopic Detection, Hematological Evaluation and Molecular Characterization of Piroplasms from Naturally Infected Dogs in Rio de Janeiro, Brazil.

Acta Parasitol 2021 Jun 15. Epub 2021 Jun 15.

Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Rua Professor Hernani Mello, São Domingos, Niterói, Rio de Janeiro, 24210-130, Brasil.

Purpose: This study aimed to analyze the frequency of piroplasmids in the blood of dogs in Rio de Janeiro, compare the performance of microscopic techniques, assess the risk factors associated with infections and also molecularly and morphologically characterize the piroplasmids identified.

Methods: In all, 407 blood samples were collected from dogs between 2018 and 2019. These were subjected to microscopic parasitological techniques for thin and thick smears, stained with Giemsa and using a rapid staining kit. Read More

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Complete genome sequence of hollyhock vein yellowing virus, a novel monopartite begomovirus infecting hollyhock in Pakistan.

Arch Virol 2021 Jun 11. Epub 2021 Jun 11.

Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.

Hollyhock (Alcea rosea, family Malvaceae) is an ornamental plant grown widely in gardens across South Asia. In a bed of ornamental plants near the village of Chakri (Punjab Province, Pakistan) in 2014, hollyhock plants showing two distinct symptom types were identified: yellow vein mosaic and leaf crumple. PCR amplification with universal primers amplified a begomovirus from separate nucleic acid extracts of single plants of each type but amplified a betasatellite only from the plant with the yellow vein mosaic symptoms. Read More

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First report of Meloidogyne arenaria infecting Maidong (Ophiopogon japonicus) in China.

Plant Dis 2021 Jun 11. Epub 2021 Jun 11.

Southwest Foresttry UniversityKunming, Yunnan, China, 650224;

Maidong (Ophiopogon japonicus) is a perennial evergreen plant of the Asparagaceae, occurring mainly in China, Japan, Vietnam, and India. It grows in the damp place on the hillside below 2000 meters above sea level, under the forest or beside the stream;It has been widely cultivated in the Sichuan ofhina for medicinal uses; and it is included in the Chinese Pharmacopoeia. During April 2019, Maidong plants exhibiting symptoms of stunting, leaf wilting, and multiple galls in the roots associated with root-knot nematode (Meloidogyne sp. Read More

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First report of stem rust, caused by Puccinia graminis, and crown rust, caused by Puccinia coronata var. avenae f. sp. avenae, on tall fescue (Festuca arundinacea) in Georgia, USA.

Plant Dis 2021 Jun 11. Epub 2021 Jun 11.

University of Georgia, 1355, 1109 Experiment Street, Griffin, Griffin, Georgia, United States, 30223.

Stem rust, caused by Puccinia graminis, and crown rust, caused by P. coronata, are common rust diseases on cool-season grasses (Karakkat et al. 2018), for which long-distance spore dispersal was recorded in northern US (Harder and Haber 1992). Read More

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Profiling system of oral microbiota utilizing polymerase chain reaction-restriction fragment length polymorphism analysis.

J Oral Biosci 2021 Jun 7. Epub 2021 Jun 7.

Division of Clinical Chemistry, Niigata University Graduate School of Health Sciences, Niigata 951-8518, Japan. Electronic address:

Objectives: Profiling of oral microbiota has traditionally been performed using conventional methods. These methods are relatively time-consuming and labor-intensive. Metagenomic analysis of oral microbiota using high-speed next-generation sequencing is a highly promising technology. Read More

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First report of strains related to the phytoplasma associated with Tanzanian Lethal Decline on on the western coast of Madagascar.

Plant Dis 2021 Jun 9. Epub 2021 Jun 9.

MINAE, DPV, Antananarivo, Madagascar;

Madagascar is a high diversity hotspot in the world, and palms are highly represented with nearly 200 endemic species (Rakotoarinivo et al., 2014). Coconut tree (Cocos nucifera) could have been introduced in Madagascar by Austronesians around AD 400 or 700 (Beaujard, 2011). Read More

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First report of pearl millet bacterial leaf blight caused by Pantoea stewartii subspecies indologenes in India.

Plant Dis 2021 Jun 9. Epub 2021 Jun 9.

University of Mysore, 29243, Applied Botany and Biotechnology, Mysore, Karnataka, India;

Pearl millet (Cenchrus americanus L.) field-grown plants of cv. 7042S shown unusual water-soaked lesions on leaf tips spreading towards the leaf base from Manasagangothri region (12. Read More

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First Report of Aloe vera Rust Caused by Uromyces aloes in an Ornamental Nursery in the United States.

Plant Dis 2021 Jun 8. Epub 2021 Jun 8.

Penn State University, Plant Pathology, University Park, Pennsylvania, United States;

Aloe vera (L.) Burm. f. Read More

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First report of Phaeoacremonium amygdalinum associated with almond dieback and wood disease in Italy.

Plant Dis 2021 Jun 8. Epub 2021 Jun 8.

University of Foggia, 18972, Department of Agriculture, Food, Natural resources and Engineering, Foggia, Puglia, Italy;

In the last 10 years, almond (Prunus dulcis (Mill.) Webb) cultivation areas in the region of Apulia (southern Italy) have increased. A recent survey in young almond orchards recorded characteristic disease symptoms including rapid collapse of branches, chlorosis of leaves (with sudden wilting and death) in June/July, and bud and shoot dieback in December-March. Read More

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First report of Nigrospora oryzae causing wilt in summer cypress (Bassia scoparia) in Pakistan.

Plant Dis 2021 Jun 7. Epub 2021 Jun 7.

University of Agriculture Faisalabad, 66724, Plant Pathology, Mehndi muhala dhobi ghat, st#2, p-126, Gojra, T. T. Singh, Faisalabad, Punjab, Pakistan, 38000.

Summer Cypress (Bassia scoparia) is a large annual herb belonging to the family Amaranthaceae native to Eurasia. It has been introduced in many other countries of the world. In Pakistan, summer cypress is also known as kochia and grown as an ornamental plant for its red fall foliage for landscapes. Read More

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First Report of Brown Leaf Spot of Caused by in China.

Plant Dis 2021 Jun 7. Epub 2021 Jun 7.

chengdu, China;

"Chuanzao 2" is a walnut variety derived from the hybridization of L. and Dode distributed in southwest China, where it is an economically important tree species in rural regions (Xiao et al. 2012). Read More

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[Study of the distribution of KIR3DL2 alleles among ethnic Han Chinese from Zhejiang].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Jun;38(6):589-592

Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou, Zhejiang 310052, China.

Objective: To study the distribution of KIR3DL2 alleles among ethnic Han Chinese from Zhejiang.

Methods: Genomic DNA was extracted by using a magnetic bead method. The full sequence of the KIR3DL2 gene was amplified with four pairs by PCR primers. Read More

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Heterologous expression and characterisation of a keratinase produced by Chryseobacterium carnipullorum.

Protein Expr Purif 2021 Jun 4;186:105926. Epub 2021 Jun 4.

Department of Microbiology and Biochemistry, PO Box 339, University of the Free State, Bloemfontein, Free State, South Africa. Electronic address:

Chryseobacterium carnipullorum 9_R23581, isolated from raw chicken meat, was evaluated for its potential to degrade keratin found in feathers. The focus of this study was to heterologously express and characterise a keratinolytic enzyme produced by C. carnipullorum. Read More

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Analysis of honey environmental DNA indicates that the honey bee (Apis mellifera L.) trypanosome parasite Lotmaria passim is widespread in the apiaries of the North of Italy.

J Invertebr Pathol 2021 Jun 4:107628. Epub 2021 Jun 4.

Department of Agricultural and Food Sciences, University of Bologna, Viale Giuseppe Fanin 46, 40127 Bologna, Italy. Electronic address:

Lotmaria passim is a trypanosomatid that infects honey bees. In this study, we established an axenic culture of L. passim from Italian isolates and then used its DNA as a control in subsequent analyses that investigated environmental DNA (eDNA) to detect this trypasonosomatid. Read More

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Single-nucleotide polymorphism detecting of some candidate genes related to lipid metabolism in Booroola Merino-Afshari sheep by Bayesian model averaging.

Trop Anim Health Prod 2021 Jun 5;53(3):342. Epub 2021 Jun 5.

Department of Animal Science, Faculty of Agriculture, University of Tabriz, 5166616471, Tabriz, Iran.

This study was designed to identify single-nucleotide polymorphisms (SNPs) of some candidate genes related to lipid metabolism and their association with carcass fat in male crossbred lambs. Hence, 96 of almost 11-month-old Booroola Merino-Afshari crossbred lambs (first-generation backcross) were used by considering their phenotypic carcass traits. Then, DNA was extracted and DNA targets were amplified using designed specific primers by PCR procedure. Read More

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A Molecular Approach for the Detection and Quantification of (Herbst) Infestation in Stored Wheat Flour.

Food Technol Biotechnol 2021 Mar;59(1):112-121

Department of Primary Processing, Storage and Handling, Indian Institute of Food Processing Technology, 613005 Thanjavur, Tamil Nadu, India.

Research Background: The presence of insect fragments is one of the major constrains in stored food commodities and it causes considerable loss in the quality of the produce. The management of the pest is viewed as a huge challenge in foodprocessingindustry. Conventionally, the detection of in the food processing industry is carried out by acid hydrolysis and staining methods that are time consuming and lack precision. Read More

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First report of tilapia lake virus emergence in fish farms in the department of Córdoba, Colombia.

Vet World 2021 Apr 10;14(4):865-872. Epub 2021 Apr 10.

Institute of Biological Research of the Tropic, University of Córdoba, Colombia.

Background And Aim: In 2016, the tilapia-producing farms in the department of Córdoba, Colombia, had witnessed outbreaks of disease with clinical signs compatible with those caused by the tilapia lake virus (TiLV). This study was conducted to confirm the presence of TiLV in some fish farms in the department of Córdoba.

Materials And Methods: A descriptive cross-sectional study was conducted in seven farms using a non-random sampling method from July 2016 to December 2017. Read More

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A targeted ancestry informative InDels panel on capillary electrophoresis for ancestry inference in Asian populations.

Electrophoresis 2021 Jun 3. Epub 2021 Jun 3.

West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, P. R. China.

CE is the primary methodology used in forensic DNA typing. Alleles of commonly used types of genetic markers could be separated and detected via CE based on dye color and migration time. Insertion/deletion (InDel) is an ideal genetic marker for forensic DNA analysis due to their abundance in the human genome, low mutation rate, availability of their allele types via CE, and elimination of stutter peaks. Read More

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Identification of Colletotrichum liriopes as the Causative Agent of Anthracnose in Buckwheat (Fagopyrum esculentum) in China.

Plant Dis 2021 Jun 2. Epub 2021 Jun 2.

Lanzhou University, Pastoral Agriculture Sci. & Tech., Jiayuguan Xilu 768, Lanzhou, Gansu, China, 730020;

Buckwheat (Fagopyrum esculentum), belonging to the Polygonaceae family, is one of the most important "functional food" crops in China. In fall 2020, buckwheat plants grown in field exhibiting stem canker symptoms were found in Tongxin county, Ningxia province, China. Symptoms included stem canker, dieback and extensive vascular discoloration. Read More

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