28,356 results match your criteria amniotic fluid

Placental lesions and SARS-Cov-2 infection: Diffuse placenta damage associated to poor fetal outcome.

Placenta 2021 Jul 15;112:97-104. Epub 2021 Jul 15.

Institut de Pathologie Multisite des Hospices Civils de Lyon, 69677, Bron Cedex, Lyon, France; SOFFOET-Société Française de Fœtopathologie, 75014, Paris, France; Université Claude Bernard Lyon 1, Faculté de Médecine Lyon Est, 69008, France.

Introduction: Pregnant women with covid-19 are more likely to experience preterm birth. The virus seems to be associated with a wide range of placental lesions, none of them specific.

Method: We collected cases of Covid-19 maternal infection during pregnancy associated with poor pregnancy outcomes, for which we received the placenta. Read More

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Amphiregulin stimulates human chorionic gonadotropin expression by inducing ERK1/2-mediated ID3 expression in trophoblast cells.

Placenta 2021 Jul 23;112:73-80. Epub 2021 Jul 23.

Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. Electronic address:

Introduction: The human chorionic gonadotropin (hCG) is a dimer consisting of an α subunit and a β subunit which is encoded by the CGB gene and is unique to hCG. hCG is a hormone mainly synthesized by syncytiotrophoblast cells in the placenta, plays a critical role in stimulating progesterone production that is necessary for maintaining normal pregnancy in the early stage. Epidermal growth factor receptor (EGFR) belongs to the receptor tyrosine kinase family which has been shown to regulate various physiological and pathological events. Read More

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A comprehensive and universal approach for embryo testing in patients with different genetic disorders.

Clin Transl Med 2021 Jul;11(7):e490

Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.

Background: In vitro fertilization (IVF) with preimplantation genetic testing (PGT) has markedly improved clinical pregnancy outcomes for carriers of gene mutations or chromosomal structural rearrangements by the selection of embryos free of disease-causing genes and chromosome abnormalities. However, for detecting whole or segmental chromosome aneuploidies, gene variants or balanced chromosome rearrangements in the same embryo require separate procedures, and none of the existing detection platforms is universal for all patients with different genetic disorders.

Methods: Here, we report a cost-effective, family-based haplotype phasing approach that can simultaneously evaluate multiple genetic variants, including monogenic disorders, aneuploidy, and balanced chromosome rearrangements in the same embryo with a single test. Read More

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Mesenchymal stem cells from different sources show distinct therapeutic effects in hyperoxia-induced bronchopulmonary dysplasia in rats.

J Cell Mol Med 2021 Jul 29. Epub 2021 Jul 29.

Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Mesenchymal stem cells (MSCs) have been shown as an effective medicinal means to treat bronchopulmonary dysplasia (BPD). The widely used MSCs were from Wharton's jelly of umbilical cord (UC-MSCs) and bone marrow (BM-MSCs). Amniotic fluid MSCs (AF-MSCs) may be produced before an individual is born to treat foetal diseases by autoplastic transplantation. Read More

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Gilbert's Syndrome With Diabetes Mellitus.

Cureus 2021 Jul 22;13(7):e16557. Epub 2021 Jul 22.

Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, CHN.

Gilbert's syndrome (GS) could lead to the high bilirubin, and gestational diabetes mellitus might reverse this index. A primigravida with a pregnancy of 38 weeks and 4 days was identified having gestational diabetes mellitus (GDM) with Gilbert's syndrome. General laboratory tests were normal except mild unconjugated hyperbilirubinaemia and hyperglycemia. Read More

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Birth weight and intra-amniotic inflammatory and infection-related complications in pregnancies with preterm prelabor rupture of membranes: a retrospective cohort study.

J Matern Fetal Neonatal Med 2021 Jul 28:1-11. Epub 2021 Jul 28.

Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czechia.

Objective: To assess the association between the birth weight of newborns and microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation in pregnancies with preterm prelabor rupture of membranes.

Methods: A total of 528 pregnancies with preterm prelabor rupture of membranes were included in this retrospective cohort study. Transabdominal amniocentesis to determine the presence of MIAC (through culturing and molecular biology methods) and intra-amniotic inflammation (according to amniotic fluid interleukin-6 level) was performed as part of standard clinical management. Read More

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Analysis of microbial differences in amniotic fluid between advanced and normal age pregnant women.

J Transl Med 2021 Jul 27;19(1):320. Epub 2021 Jul 27.

Department of Prenatal Diagnosis, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, 210004, China.

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Vaginal misoprostol versus intracervical Foley catheter for cervical ripening in postdate primigravid women: a randomized clinical trial.

BMC Pregnancy Childbirth 2021 Jul 27;21(1):533. Epub 2021 Jul 27.

The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran.

Background: Being one of the most common indications of labor induction, postdate pregnancy can lead to serious maternal and fetal complications. In this study we aimed to compare vaginal misoprostol with intracervical Foley catheter (FC) for cervical ripening in postdate primigravid women.

Methods: This randomized clinical trial included 120 primigravid women aged 18-35 years with singleton,  postdate pregnancies, and Bishop score ≤ 4. Read More

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The role of echocardiography in amniotic fluid embolism: a case series and review of the literature.

Can J Anaesth 2021 Jul 26. Epub 2021 Jul 26.

Division of Critical Care, Department of Medicine, Jewish General Hospital, McGill University, 3755 ch. de la Côte-Sainte-Catherine Rd, Suite H-364.1, Montreal, QC, H3T 1E2, Canada.

Purpose: Amniotic fluid embolism (AFE) is a rare, but often fatal condition characterized by sudden hemodynamic instability and coagulopathy occurring during labour or in the early postpartum period. As the mechanisms leading to shock and the cardiovascular effects of AFE are incompletely understood, the purpose of this case series is to describe how AFE presents on echocardiography and review limited reports in the literature.

Clinical Features: We describe three cases of AFE at the Jewish General Hospital, a tertiary care centre in Montreal, Canada. Read More

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Longitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one-time oral dosing in pregnancy.

Clin Transl Sci 2021 Jul 26. Epub 2021 Jul 26.

Department of Clinical Pharmacology and Experimental Therapeutics, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.

To utilize noninvasive collection of amniotic fluid in the setting of preterm premature rupture of membranes (PPROMs) to report the time concentration profile of azithromycin in amniotic fluid over 7 days from a single dose, and evaluate the correlation between azithromycin concentration and inflammatory markers in amniotic fluid. Prospective cohort study of five pregnant patients admitted with PPROMs and treated with a single 1 g oral azithromycin dose. Amniotic fluid was collected from pads and used to quantify azithromycin concentration as well as TNFa, IL-1a, IL-1b, IL-6, IL-8, and IL-10 concentrations. Read More

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The Inflammatory Milieu of Amniotic Fluid Increases with Chorio-Deciduitis Grade in Inflammation-Restricted to Choriodecidua, but Not Amnionitis, of Extra-Placental Membranes.

J Clin Med 2021 Jul 8;10(14). Epub 2021 Jul 8.

Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul 03080, Korea.

No information exists about whether intra-amniotic inflammatory response increases with a chorio-deciduitis grade in the context of both inflammation-restricted to chorio-decidua and amnionitis of extra-placental membranes among spontaneous preterm births. The objective of current study is to examine this issue. A study population included 195 singleton pregnant women with chorio-deciduitis, and who spontaneously delivered at preterm (21. Read More

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Dynamic Changes of Metabolite Profiles in Maternal Biofluids During Gestation Period in Huanjiang Mini-Pigs.

Front Vet Sci 2021 6;8:636943. Epub 2021 Jul 6.

Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.

The biochemical parameters related to nitrogenous metabolism in maternal biofluids may be linked and even reflect the fetal metabolism and growth. The present study have measured the concentrations of various parameters related to amino acid (AA) and lipid metabolism, as well as different metabolites including the free AAs in maternal plasma and amniotic and allantoic fluid corresponding to fetuses with different body weight (BW) during different gestation periods, in order to identify the possible relationships between biochemical parameters and fetal growth. A total of 24 primiparous Huanjiang mini-pigs were fed with a standard diet. Read More

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Prenatal case of Simpson-Golabi-Behmel syndrome with a de novo 370Kb-sized microdeletion of Xq26.2 compassing partial GPC3 gene and review.

Mol Genet Genomic Med 2021 Jul 22:e1750. Epub 2021 Jul 22.

Department of Medical Genetics, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, Hunan, China.

Background: Simpson-Golabi-Behmel syndrome type 1 (SGBS1) is a rare X-linked recessive disorder characterized by pre- and postnatal overgrowth and a broad spectrum of anomalies including craniofacial dysmorphism, heart defects, renal, and genital anomalies. Due to the ultrasound findings are not pathognomonic for this syndrome, most clinical diagnosis of SGBS1 are made postnatally.

Methods: A pregnant woman with abnormal prenatal sonographic findings was advised to perform molecular diagnosis. Read More

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Molecular evidence suggesting the persistence of residual SARS-CoV-2 and immune responses in the placentas of pregnant patients recovered from COVID-19.

Cell Prolif 2021 Jul 22:e13091. Epub 2021 Jul 22.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Objectives: Recent studies have shown the presence of SARS-CoV-2 in the tissues of clinically recovered patients and persistent immune symptoms in discharged patients for up to several months. Pregnant patients were shown to be a high-risk group for COVID-19. Based on these findings, we assessed SARS-CoV-2 nucleic acid and protein retention in the placentas of pregnant women who had fully recovered from COVID-19 and cytokine fluctuations in maternal and foetal tissues. Read More

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Neonatal polycystic kidney disease: a novel variant.

BMJ Case Rep 2021 Jul 21;14(7). Epub 2021 Jul 21.

Obstetrics and Gynaecology, Royal College of Surgeons in Ireland, Dublin, Ireland.

Polycystic kidney disease (PKD) is a condition typified by multiple renal cysts and renal enlargement. Classification is usually determined by mode of inheritance-autosomal dominant PKD (ADPKD) or autosomal recessive PKD (ARPKD). ARPKD frequently presents in fetal life, but here we report a rare case of a family with two siblings diagnosed with ADPKD manifesting in utero with novel genetic findings. Read More

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The human amniotic fluid stem cell secretome triggers intracellular Ca oscillations, NF-κB nuclear translocation and tube formation in human endothelial colony-forming cells.

J Cell Mol Med 2021 Jul 20. Epub 2021 Jul 20.

Department of Biology and Biotechnology "Lazzaro Spallanzani", Laboratory of General Physiology, University of Pavia, Pavia, Italy.

Second trimester foetal human amniotic fluid-derived stem cells (hAFS) have been shown to possess remarkable cardioprotective paracrine potential in different preclinical models of myocardial injury and drug-induced cardiotoxicity. The hAFS secretome, namely the total soluble factors released by cells in their conditioned medium (hAFS-CM), can also strongly sustain in vivo angiogenesis in a murine model of acute myocardial infarction (MI) and stimulates human endothelial colony-forming cells (ECFCs), the only truly recognized endothelial progenitor, to form capillary-like structures in vitro. Preliminary work demonstrated that the hypoxic hAFS secretome (hAFS-CM ) triggers intracellular Ca oscillations in human ECFCs, but the underlying mechanisms and the downstream Ca -dependent effectors remain elusive. Read More

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Intra-amniotic Injection of Mouse Embryos.

Bio Protoc 2018 May 20;8(10):e2854. Epub 2018 May 20.

Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center of Brain Science, Key Laboratory of Medical Molecular Virology of Ministry of Education/Ministry of Health and Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Recent outbreaks of infectious neuro-developmental diseases such as congenital Zika syndrome - have led to a demand for prognosis data from animal models. We developed an intra-amniotic injection mice model that allows Zika virus (ZIKV) infected mice to grow to puberty. In this system, ZIKV is injected into the amniotic fluid of pregnant mice and infected embryos thereafter. Read More

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Disseminated intravascular coagulation type of amniotic fluid embolism: a challenging case report with favorable outcome.

Pan Afr Med J 2021 1;38:325. Epub 2021 Apr 1.

Anesthesia-Intensive Care Department B, Maternity and Neonatology Teaching Center of Monastir, Monastir, Tunisia.

Amniotic fluid embolism (AFE) is an unforeseeable, life-threatening complication of pregnancy and child birth. Although rare in an absolute sense, most contemporary series of maternal deaths from developed countries report AFE as a leading cause of mortality in the pregnant population. It has a heterogeneous presentation. Read More

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Classifying and Evaluating Fetuses With Ventriculomegaly in Genetic Etiologic Studies.

Front Genet 2021 2;12:682707. Epub 2021 Jul 2.

Medical Genetic Diagnosis and Therapy Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fujian Key Laboratory for Prenatal Diagnosis and Birth Defect, Fuzhou, China.

The association between genetics and fetuses with ventriculomegaly (VM) is unknown. This study aimed to classify and evaluate abnormal copy number variations (CNVs) in fetuses with VM. From December 2016 to September 2020, amniotic fluid or umbilical cord blood from 293 pregnant women carrying fetuses with VM was extracted for single-nucleotide polymorphism microarray (SNP array). Read More

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Current and Emerging Strategies for Prediction and Diagnosis of Prelabour Rupture of the Membranes: A Narrative Review.

Saadia Ghafoor

Malays J Med Sci 2021 Jun 30;28(3):5-17. Epub 2021 Jun 30.

Kakshal Hospital, Kakshal, Peshawar, Khyber Pakhtunkhwa, Pakistan.

Prelabour rupture of membranes (PROM) refers to the disruption of foetal membranes before the onset of labour, resulting in the leakage of amniotic fluid. PROM complicates 3% and 8% of preterm and term pregnancies, respectively. Accurate and timely diagnosis is crucial for effective management to prevent adverse maternal- and foetal-outcomes. Read More

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Clinical significance and mechanisms associated with segmental UPD.

Mol Cytogenet 2021 Jul 20;14(1):38. Epub 2021 Jul 20.

Cytogenetics Department, Laboratory Corporation of America, Research Triangle Park, NC, 27709, USA.

Whole chromosome uniparental disomy (UPD) has been well documented with mechanisms largely understood. However, the etiology of segmental limited UPD (segUPD) is not as clear. In a 10-year period of confirming (> 300) cases of whole chromosome UPD, we identified 86 segmental cases in both prenatal and postnatal samples. Read More

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Vitelline Vessel Remnant-Derived Funisitis Is a Sensitive and Specific Predictor of Histological Evidence of Amniotic Fluid Infection.

Pediatr Dev Pathol 2021 Jul 21:10935266211033271. Epub 2021 Jul 21.

Department of Pathology & Laboratory Medicine, University of Calgary Cumming School of Medicine, Calgary, Alberta, Canada.

Background: 4-11% of umbilical cords contain vitelline vessel remnants (VVRs). A recent study has described neutrophilic inflammation arising from VVRs and suggested an association with amniotic fluid infection (AFI).

Methods: During routine placental pathology sign-out over a six month period, we identified 70 cords with VVRs. Read More

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Maternal Rat Metabolomics: Amniotic Fluid and Placental Metabolic Profiling Workflows.

J Proteome Res 2021 Jul 20. Epub 2021 Jul 20.

UMR 1253 iBrain, Université de Tours, Inserm, Tours 37000, France.

Studying the metabolome of specific gestational compartments is of growing interest in the context of fetus developmental disorders. However, the metabolomes of the placenta and amniotic fluid (AF) are poorly characterized. Therefore, we present the validation of a fingerprinting methodology. Read More

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Role of Intravenous Magnesium Sulphate in Term Neonates with Hypoxic Ischemic Encephalopathy (HIE) in a Low-income Country: A Randomised Clinical Trial.

J Coll Physicians Surg Pak 2021 Jul;30(7):817-820

Department of Paediatric Medicine Unit-II, Services Hospital, Lahore, Pakistan.

Objective: To determine the effects of magnesium sulphate in term neonates with hypoxic ischemic encephalopathy (HIE) in reducing mortality and morbidity.

Study Design: Randomised clinical trial.

Place And Duration Of Study: Department of Neonatology, Services Hospital, Lahore, Pakistan from April to December 2019. Read More

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Comparing effects and action mechanisms of BPA and BPS on HTR-8/SVneo placental cells.

Biol Reprod 2021 Jul 16. Epub 2021 Jul 16.

University of Bologna, Department of Biological, Geological, and Environmental Sciences, Ravenna, Italy.

Bisphenol A (BPA) is one of the most investigated compound as a suspected endocrine disrupting chemical. It has been found at nM concentrations in the maternal serum, cord serum, and amniotic fluid and also permeates placental tissues. Attempts are being made to replace BPA with the analog Bisphenol S (BPS). Read More

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The Relationship between Prenatal Diagnosis Indications and Abnormal Chromosomal Karyotypes: A retrospective cohort of 4646 cases in Beijing from 2012-2019.

Curr Top Med Chem 2021 Jul 14. Epub 2021 Jul 14.

Clinical Laboratory, First Medical Center of Chinese PLA General Hospital, Beijing, China.

Background And Objective: Chromosomal abnormalities are genetic disorders caused by abnormal chromosome number or structure and can endanger multiple organs, morphology, and function of the systems. This study aims to investigate the relationship between prenatal diagnosis indications and abnormal karyotypes to improve prenatal screening.

Methods: The karyotype analyses were carried out on 4646 pregnant women with prenatal diagnosis indications referred to the first medical center of Chinese PLA General Hospital from 2012 to 2019. Read More

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Viral populations of SARS-CoV-2 in upper respiratory tract, placenta, amniotic fluid and umbilical cord blood support viral replication in placenta.

Clin Microbiol Infect 2021 Jul 11. Epub 2021 Jul 11.

Microbiology Department, Vall D'Hebron Institut de Recerca (VHIR), Vall D'Hebron Hospital Universitari, Vall D'Hebron Barcelona Hospital Campus, Barcelona, Spain.

A woman at the first stage of labour infected with SARS-CoV-2 was admitted to our hospital with symptomatology compatible with mild respiratory disease. Respiratory and non-respiratory samples from the mother were all positive for the virus, but not from the baby. Laboratory tests rejected vertical transmission. Read More

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Probable viremia and positive placental swabs for SARS-CoV-2 in a preterm pregnant woman with mild COVID-19.

J Med Virol 2021 Jul 14. Epub 2021 Jul 14.

Department of Obstetrics and Gynaecology, School of Medicine, Zonguldak Bulent Ecevit University, Zonguldak, Turkey.

This study aimed to report a case of mild novel coronavirus disease (COVID-19) in a pregnant woman with probable viremia, as reverse transcription-polymerase chain reaction (RT-PCR) testing of endometrial and placental swabs for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was positive. A 26-year-old multigravida at 35 weeks 2 days of gestation, who had extensive thigh and abdominal cellulitis, tested SARS-CoV-2 positive by RT-PCR performed on samples from the endometrium and maternal side of the placenta. However, other samples (amniotic fluid, fetal side of the placenta, umbilical cord, maternal vagina, and neonatal nasopharynx) tested negative for SARS-CoV-2. Read More

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Application of Multiple Short Tandem Repeat Loci for Rapid Diagnosis of Down Syndrome and Edward Syndrome.

Clin Lab 2021 Jul;67(7)

Background: Chromosomal diseases with chromosomal abnormalities are one of the most common genetic diseases in humans, including abnormal numbers and structural abnormalities. Patau syndrome (also known as trisomy 13), Edward syndrome (also called trisomy 18), and Down syndrome (also known as trisomy 21) are all clinically fatal diseases caused by abnormal numbers of autosomes. However, there is no reliable and effective cure for chromosomal diseases, mainly relying on fast and accurate prenatal diagnosis technology to reduce the rate of birth defects. Read More

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