1,572,060 results match your criteria amino acid


Characterizing unexpected interactions of a glutamine transporter inhibitor with members of the SLC1A transporter family.

J Biol Chem 2022 Jun 22:102178. Epub 2022 Jun 22.

School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, NSW, Australia. Electronic address:

The Solute Carrier 1A (SLC1A) family comprises a group of membrane proteins that act as dual-function amino acid transporters and chloride channels and includes the alanine serine cysteine transporters (ASCTs) as well as the excitatory amino acid transporters (EAATs). ASCT2 is regarded as a promising target for cancer therapy, as it can transport glutamine and other neutral amino acids into cells and is upregulated in a range of solid tumors. The compound L-γ-glutamyl-p-nitroanilide (GPNA) is widely used in studies probing the role of ASCT2 in cancer biology; however, the mechanism by which GPNA inhibits ASCT2 is not entirely clear. Read More

View Article and Full-Text PDF

Mutation of the α5 nicotinic acetylcholine receptor subunit increases ethanol and nicotine consumption in adolescence and impacts adult drug consumption.

Neuropharmacology 2022 Jun 22:109170. Epub 2022 Jun 22.

Pharmacology Graduate Group, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, USA; Department of Psychiatry, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, USA. Electronic address:

Alcohol and nicotine are commonly used during adolescence, establishing long-lasting neuroplastic alterations that influence subsequent drug use and abuse. Drinking- and smoking-related traits have been extensively associated with variation in CHRNA5 - the gene that encodes the α5 subunit of neuronal nicotinic acetylcholine receptors (nAChRs). The single nucleotide polymorphism (SNP) rs16969968 in CHRNA5 encodes an amino acid substitution (D398N) that alters the function and pharmacokinetics of α5-containing nAChR. Read More

View Article and Full-Text PDF

Effects of different types of nitrogen sources in water on the formation potentials of nitrogenous disinfection by-products in chloramine disinfection process based on isotope labeling.

Sci Total Environ 2022 Jun 22:156692. Epub 2022 Jun 22.

Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China; Engineering Research Center for Water Pollution Source Control & Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China. Electronic address:

Nitrogenous disinfection by-products (N-DBPs) raise increasing concerns because of their high genotoxicity, cytotoxicity, and carcinogenicity compared to carbonaceous disinfection by-products (C-DBPs). Nitrogen-containing disinfectants, dissolved organic nitrogen (DON), and inorganic nitrogen may all promote the formation of N-DBPs. Therefore, it is urgent to explore the dominant nitrogen source of N-DBPs under the coexistence of multiple nitrogen sources. Read More

View Article and Full-Text PDF

Evaluation of crotamine based probes as intracellular targeted contrast agents for magnetic resonance imaging.

Bioorg Med Chem 2022 Jun 15;69:116863. Epub 2022 Jun 15.

High-Field MR Center, Max Planck Institute for Biological Cybernetics, Tübingen, Germany.

Crotamine is a lysine and cysteine rich 42 amino acids long bio-active polypeptide, isolated from the venom of a South American rattlesnake, that can also be used as cell penetrating peptide. A facile synthetic scheme for coupling cargo molecules like fluorophores (carboxyfluorescein) or MRI probes (Gd-DO3A-based macrocycle) is presented. The toxicity, cellular internalization and steady-state accumulation after long-term incubation for 18 h, as well as magnetic resonance relaxivities and cellular relaxation rates of crotamine based probes were evaluated and compared to its shorter synthetic fragment CyLoP-1. Read More

View Article and Full-Text PDF

Characterization of novel antimicrobial peptides designed on the basis of amino acid sequence of peptides from egg white hydrolysate.

Int J Food Microbiol 2022 Jun 18;378:109802. Epub 2022 Jun 18.

Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address:

Salmonella enterica subsp. enterica serotype Typhimurium (S. Typhimurium) is one of the most prevalent foodborne pathogens responsible for food poisoning and is spread through the consumption of contaminated poultry products. Read More

View Article and Full-Text PDF

Mechanism of drug resistance of BVDV induced by F224S mutation in RdRp: A case study of VP32947.

Comput Biol Chem 2022 Jun 20;99:107715. Epub 2022 Jun 20.

Gansu Province Livestock Embryo Engineering Research Center, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address:

Bovine viral diarrhea virus (BVDV) is an enveloped virus with an RNA genome, causing serious economic losses to the areas dominated by livestock industry. Currently, although several compounds with biological activities of inhibiting virus replication have been reported, amino acid mutations (especially F224S mutation) frequently occurring in the RNA-dependent RNA polymerase (RdRp) have greatly reduce their value of further research. In this study, we introduced an effective and rapid in silico strategy to explore the differences in the binding modes of VP32947 between the wild/mutant-type RdRp at the molecular level, and further explained the main reasons for the variations in the inhibitory activities of VP32947 against the two types of enzymes. Read More

View Article and Full-Text PDF

Polystyrene nanoplastics induce profound metabolic shift in human cells as revealed by integrated proteomic and metabolomic analysis.

Environ Int 2022 Jun 15;166:107349. Epub 2022 Jun 15.

School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. Electronic address:

Nanoplastics (NPLs) are widespread in our environment. However, their impacts on human health and precise toxicity mechanisms remain poorly understood. Here we studied the internalization, release, and cytotoxicity of polystyrene nanoplastics (PSNPs) using the renal tubular epithelial cell line HKC and human derived liver cell line HL-7702. Read More

View Article and Full-Text PDF

Bioinspired Palladium-Catalyzed Intramolecular C(sp3)-H Activation for the Collective Synthesis of Proline Natural Products.

Angew Chem Int Ed Engl 2022 Jun 25. Epub 2022 Jun 25.

Shanghai Jiao Tong University, School of Chemistry and Chemical Engineering, 800 Dongchuan Road, B329 Chemsitry Building, Shanghai Jiao Tong University, 200240, Shanghai, CHINA.

Bioinspired palladium-catalyzed intramolecular cyclization of amino acid derivatives containing a vinyl iodide moiety by C-H activation enabled rapid access to a wide range of functionalized proline derivatives with an exocyclic olefin. To demonstrate the practicality of this methodology, the functionalized prolines were used as intermediates for the synthesis of several natural products: lucentamycin A, oxotomaymycin, oxoprothracarcin, and barmumycin. Read More

View Article and Full-Text PDF

Impact of Increasing Standardized Ileal Digestible Valine:Lysine in Diets Containing 30% Dried Distiller Grains with Solubles on Growing Pig Performance.

J Anim Sci 2022 Jun 25. Epub 2022 Jun 25.

Christensen Farms, Sleepy Eye, MN 56085, United States.

A total of 2,430 pigs (DNA 600 x Topigs Norsvin 70, initially 39.4 kg) were used in a 28-d trial to determine the standardized ileal digestible (SID) Val:Lys requirement for pigs fed diets containing 30% DDGS. Treatments included 5 diets containing 30% DDGS with SID Val:Lys ratios of 60, 65, 70, 75, and 80%, plus a corn soybean meal (SBM) diet, for a total of 6 dietary treatments. Read More

View Article and Full-Text PDF

Imputation of the major histocompatibility complex region identifies major independent variants associated with bullous pemphigoid and dermatomyositis in Han Chinese.

J Dermatol 2022 Jun 25. Epub 2022 Jun 25.

Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

As autoimmune skin diseases, both bullous pemphigoid (BP) and dermatomyositis (DM) show significant associations with the major histocompatibility complex (MHC) region. In fact, the coexistence of BP and DM has been previously reported. Therefore, we hypothesized that there may be a potential genetic correlation between BP and DM. Read More

View Article and Full-Text PDF

Variant Annotation and Functional Prediction: SnpEff.

Authors:
Pablo Cingolani

Methods Mol Biol 2022 ;2493:289-314

AstraZeneca, Oncology R&D, Arlington, MA, USA.

Variant annotations, in general, refer to the process of information enrichment of genomic variants from a sequencing experiment. Typically these annotations include functional predictions, such as predicting the amino acid sequence changes from the DNA variant, predicting whether the variant will induce a splice anomaly, or predicting nonsense mediated decay. But other annotations also include combining with genomic databases, adding conservation scores, or comparing to allele frequencies from large population databases. Read More

View Article and Full-Text PDF
January 2022

Essential role of aerobic glycolysis in epithelial-to-mesenchymal transition during carcinogenesis.

Clin Transl Oncol 2022 Jun 25. Epub 2022 Jun 25.

Department of Medical Oncology, Dr. BRA IRCH, All India Institute of Medical Sciences, 110029, New Delhi, India.

Epithelial-to-mesenchymal transition (EMT) confers the most lethal characteristics to cancer cells i.e., metastasis and resistance to chemo-and-radio-therapy, and therefore exhibit an appealing target in the field of oncology. Read More

View Article and Full-Text PDF

Protective effect of the stressed supernatant from Lactococcus lactis subsp. lactis and its metabolic analysis.

Arch Microbiol 2022 Jun 25;204(7):428. Epub 2022 Jun 25.

School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.

There are numerous factors restricting wide application of lactic acid bacteria (LAB) in dairy industry, causing urgent demands for novel bioprotectants. Protective effects and metabolites of Lactococcus lactis subsp. lactis (L. Read More

View Article and Full-Text PDF

Exposure of Candida parapsilosis to the silver(I) compound SBC3 induces alterations in the proteome and reduced virulence.

Metallomics 2022 Jun 25. Epub 2022 Jun 25.

SSPC Pharma Research Centre, Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland.

The antimicrobial properties of silver have been exploited for many centuries and continue to gain interest in the fight against antimicrobial drug resistance. The broad-spectrum activity and low toxicity of silver have led to its incorporation into a wide range of novel antimicrobial agents, including N-heterocyclic carbene (NHC) complexes. The antimicrobial activity and in vivo efficacy of the NHC silver(I) acetate complex SBC3, derived from 1,3-dibenzyl-4,5-diphenylimidazol-2-ylidene (NHC*), has previously been demonstrated, although the mode(s) of action of SBC3 remains to be fully elucidated. Read More

View Article and Full-Text PDF

Molecular basis and functional development of enzymes related to amino acid metabolism.

Authors:
Tohru Yoshimura

Biosci Biotechnol Biochem 2022 Jun 25. Epub 2022 Jun 25.

Graduate School of Bioagricultural Sciences, Nagoya University.

Enzymology, the study of enzyme structures and reaction mechanisms can be considered a classical discipline. However, enzymes cannot be freely designed to catalyze desired reactions yet, and enzymology is by no means a complete science. I have long studied the reaction mechanisms of enzymes related to amino acid metabolism, such as aminotransferases and racemases, which depend on pyridoxal 5'-phosphate, a coenzyme form of vitamin B6. Read More

View Article and Full-Text PDF

Bringing it in: a transporter of extracellular amino acids for regulation of plant immunity.

Authors:
Jian Chen

Plant Physiol 2022 Jun 25. Epub 2022 Jun 25.

International Genome Center, Jiangsu University, Zhenjiang 212013, China.

View Article and Full-Text PDF

Exploration of Lactiplantibacillus fabifermentans and Furfurilactobacillus rossiae as potential cocoa fermentation starters.

J Appl Microbiol 2022 Jun 25. Epub 2022 Jun 25.

Dipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente, Università degli Studi di Milano, Milan, Italy.

Aims: To investigate the characteristics of two minority autochthonous LAB species, with particular regard to those properties that could be exploited in an improved cocoa fermentation process from a quality and safety point of view.

Methods And Results: Bacterial, yeast and mould strains characteristic of spontaneously fermented Dominican cocoa beans were isolated and identified by 16S or 26S rRNA gene sequencing. The potential of two autochthonous strains of LAB belonging to the species Lactiplantibacillus fabifermentans and Furfurilactibacillus rossiae were investigated. Read More

View Article and Full-Text PDF

An example of parenchymal renal sparing in the context of complex malformations due to a novel mutation in the PBX1 gene.

Birth Defects Res 2022 Jun 25. Epub 2022 Jun 25.

UOC Genetica Medica, IRCCS Istituto Giannina Gaslini, Genoa, Italy.

Introduction: PBX1 encodes the pre-B cell leukemia factor 1, a Three Amino acid Loop Extension (TALE) transcription factor crucial to regulate basic developmental processes. PBX1 loss-of-function variants have been initially described in association with renal malformations in both isolated and syndromic forms.

Case Report: Herein, we report a male infant presenting multiple organ malformations (cleidosternal dysostosis, micrognathia, left lung hypoplasia, wide interatrial defect, pulmonary hypertension, total anomalous pulmonary venous return, intestinal malrotation) and carrying the heterozygous de novo c. Read More

View Article and Full-Text PDF

Effect of glycinebetaine on metabolite profiles of cold-stored strawberry revealed by H NMR-based metabolomics.

Food Chem 2022 Nov 10;393:133452. Epub 2022 Jun 10.

Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; NUS Environmental Research Institute (NERI), #02-01, T-Lab Building (TL), 5A Engineering Drive 1, Singapore 117411, Singapore. Electronic address:

Glycinebetaine (GB) has long been used as a preservative for refrigerated fruits, but the effect of GB on the global metabolites of cold-stored strawberries is still unclear. In this study, the effects of exogenous application of GB on quality-related metabolites of cold-stored strawberries were investigated by nuclear magnetic resonance (NMR)-based metabolomic analysis. The results showed that the application of GB (especially at the concentration of 10 mM) on cold-stored strawberries effectively stabilized the sugars (d-xylose and d-glucose) and amino acids (tyrosine, leucine, and tryptophan) content, and lowered the acid (acetic acid) content as well. Read More

View Article and Full-Text PDF
November 2022

PITRM1 interaction studies with amyloidogenic nonapeptide mutants of familial Alzheimer's disease.

J Biomol Struct Dyn 2022 Jun 24:1-12. Epub 2022 Jun 24.

Neuroscience Lab, Centre for Molecular and Nanomedical Sciences, Centre for Nanoscience and Nanotechnology, School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, India.

Amyloid β-protein (ABP) is found to be the major cause for the development of neurodegeneration which leads to Alzheimer's. The Aβ nonapeptide segment, QKLVFFAED (amino acids 15-23) is the highly amyloidogenic central region of Aβ. Familial mutation in Aβ increases the aggregation property of the peptide compared to the Native (Wild) amyloid-beta (Aβ) and these mutations fall on the Aβ nonapeptide segment. Read More

View Article and Full-Text PDF

Utilization of lignocellulosic biofuel conversion residue by diverse microorganisms.

Biotechnol Biofuels Bioprod 2022 Jun 24;15(1):70. Epub 2022 Jun 24.

Department of Bacteriology, University of Wisconsin-Madison, 1550 Linden Dr, Madison, WI, 53706, USA.

Background: Lignocellulosic conversion residue (LCR) is the material remaining after deconstructed lignocellulosic biomass is subjected to microbial fermentation and treated to remove the biofuel. Technoeconomic analyses of biofuel refineries have shown that further microbial processing of this LCR into other bioproducts may help offset the costs of biofuel generation. Identifying organisms able to metabolize LCR is an important first step for harnessing the full chemical and economic potential of this material. Read More

View Article and Full-Text PDF

Mutations in PmUFGT3 contribute to color variation of fruit skin in Japanese apricot (Prunus mume Sieb. et Zucc.).

BMC Plant Biol 2022 Jun 24;22(1):304. Epub 2022 Jun 24.

College of Horticulture, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095, China.

Background: Japanese apricot (Prunus mume Sieb. et Zucc.) is popular for both ornamental and processing value, fruit color affects the processing quality, and red pigmentation is the most obvious phenotype associated with fruit color variation in Japanese apricot, mutations in structural genes in the anthocyanin pathway can disrupt the red pigmentation, while the formation mechanism of the red color trait in Japanese apricot is still unclear. Read More

View Article and Full-Text PDF

Psychobiotic Potential of Gamma-Aminobutyric Acid-Producing Marine Enterococcus faecium SH9 from Marine Shrimp.

Probiotics Antimicrob Proteins 2022 Jun 25. Epub 2022 Jun 25.

Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt.

Psychobiotics are a novel class of probiotics with potential to confer mental wellness via production of neuroactive compounds such as gamma-aminobutyric acid (GABA). The demand for new biological sources of GABA has increased steadily. Therefore, the current study reports the isolation of 17 presumptive lactic acid bacteria (LAB) from marine samples and their screening for GABA synthesis from monosodium glutamate (MSG) using thin-layer chromatography (TLC). Read More

View Article and Full-Text PDF

A multiparametric niche-like drug screening platform in acute myeloid leukemia.

Blood Cancer J 2022 Jun 24;12(6):95. Epub 2022 Jun 24.

Université Paris Cité, Génomes, biologie cellulaire et thérapeutique U944, INSERM, CNRS, F-75010, Paris, France.

Functional precision medicine in AML often relies on short-term in vitro drug sensitivity screening (DSS) of primary patient cells in standard culture conditions. We designed a niche-like DSS assay combining physiologic hypoxia (O 3%) and mesenchymal stromal cell (MSC) co-culture with multiparameter flow cytometry to enumerate lymphocytes and differentiating (CD11/CD14/CD15+) or leukemic stem cell (LSC)-enriched (GPR56+) cells within the leukemic bulk. After functional validation of GPR56 expression as a surrogate for LSC enrichment, the assay identified three patterns of response, including cytotoxicity on blasts sparing LSCs, induction of differentiation, and selective impairment of LSCs. Read More

View Article and Full-Text PDF

Clinical and genetic spectrum of Mitochondrial DNA depletion syndromes: a report of 6 cases with 4 novel variants.

Mitochondrion 2022 Jun 21. Epub 2022 Jun 21.

Cairo University Children Hospital , Pediatric Neurology and Metabolic Division, Cairo , Egypt.

Mitochondrial DNA (mtDNA) depletion syndromes (MDS) are a heterogeneous group of rare autosomal recessive genetic disorders characterized by a decrease in the number of mtDNA copies inside the organ involved. There are three distinct forms of MDS including the hepatocerebral, the myopathic and the encephalomyopathic forms. The diversity in the clinical and genetic spectrum of these disorders makes the diagnosis challenging. Read More

View Article and Full-Text PDF

Derivation of splice junction-specific antibodies using a unique hapten targeting strategy and directed evolution.

N Biotechnol 2022 Jun 21. Epub 2022 Jun 21.

Abcam, 688 East Main Street, Branford, CT 06405, USA. Electronic address:

Alternative splicing of RNA occurs frequently in eukaryotic cells and can result in multiple protein isoforms that are nearly identical in amino acid sequence, but have unique biological roles. Moreover, the relative abundance of these unique isoforms can be correlative with diseased states and potentially used as biomarkers or therapeutic targets. However, due to high sequence similarities among isoforms, current proteomic methods are incapable of differentiating native protein isoforms derived from most alternative splicing events. Read More

View Article and Full-Text PDF

Amino acid coordination complex mediates cisplatin entrapment within PEGylated liposome: an implication in colorectal cancer therapy.

Int J Pharm 2022 Jun 21:121946. Epub 2022 Jun 21.

Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Cis-Diaminedichloroplatinum (cisplatin, CDDP) remained among the most widely used anti-cancer agents; however, management of the dose-limiting side effects is still a great hurdle to its therapeutic potential. In the framework of this investigation, novel approach was developed for CDDP encasement within liposome based on the formation of a coordination bond between the platinum (II) atom and a carboxylic group in aspartic acid (AA) and glutamic acid (GA). We have also compared two methods of preparation based on equilibration and conventional lipid film hydration. Read More

View Article and Full-Text PDF

Artificial intelligence channelizing protein-peptide interactions pipeline for host-parasite paradigm in IL-10 and IL-12 reciprocity by SHP-1.

Biochim Biophys Acta Mol Basis Dis 2022 Jun 21:166466. Epub 2022 Jun 21.

National Centre for Cell Science, NCCS Complex, Ganeshkhind, SP Pune University Campus, Pune 411007, INDIA. Electronic address:

Identification of molecular targets in any cellular phenomena is a challenge and a path that one endeavors upon independently. We have identified a phosphatase SHP-1 as a point of intervention of IL-10 and IL-12 reciprocity in leishmaniasis. The therapeutic model that we have developed uniquely targets this protein but the pipeline in general can be used by the researchers for their unique targets. Read More

View Article and Full-Text PDF

Communities of niche-optimized strains (CoNoS) - Design and creation of stable, genome-reduced co-cultures.

Metab Eng 2022 Jun 21. Epub 2022 Jun 21.

Institut für Bio- und Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, Jülich, Germany. Electronic address:

Current bioprocesses for production of value-added compounds are mainly based on pure cultures that are composed of rationally engineered strains of model organisms with versatile metabolic capacities. However, in the comparably well-defined environment of a bioreactor, metabolic flexibility provided by various highly abundant biosynthetic enzymes is much less required and results in suboptimal use of carbon and energy sources for compound production. In nature, non-model organisms have frequently evolved in communities where genome-reduced, auxotrophic strains cross-feed each other, suggesting that there must be a significant advantage compared to growth without cooperation. Read More

View Article and Full-Text PDF

The antibacterial activity of a novel NK-lysin homolog and its molecular characterization and expression in the striped catfish, Pangasianodon hypophthalmus.

Fish Shellfish Immunol 2022 Jun 21. Epub 2022 Jun 21.

College of Marine Sciences, South China Agricultural University, Guangzhou, China; Guangdong Province Engineering Research Centre of Aquatic Immunization and Aquaculture Health Techniques, South China Agricultural University, Guangzhou, China; University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, China. Electronic address:

Aeromonas hydrophila was a common bacterial pathogen in aquaculture resulting in considerable losses to the striped catfish aquaculture industry. As an emergent antimicrobial peptide (AMP), NK-lysin (NKL) had activity against various microorganisms. However, the antibacterial activity of NKL from striped catfish (Pangasianodon hypophthalmus) both in vitro and vivo remains unclear. Read More

View Article and Full-Text PDF