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Dichloromethane increases mutagenic DNA damage and transformation ability in cholangiocytes and enhances metastatic potential in cholangiocarcinoma cell lines.

Chem Biol Interact 2021 Jul 17;346:109580. Epub 2021 Jul 17.

Laboratory of Environmental Toxicology, Chulabhorn Research Institute, Laksi, Bangkok, Thailand; Center of Excellence on Environmental Health and Toxicology (EHT), Thailand. Electronic address:

Dichloromethane (DCM), a widely used chlorinated solvent, is classified by IARC (2017) as probably carcinogenic to humans. Exposure to DCM has been associated with increased incidence of cholangiocarcinoma (CCA) in humans. This study aimed to investigate how DCM could contribute to CCA development by investigating the effects of DCM on DNA damage and cell transformation in cholangiocytes (MMNK-1) and on metastatic potential as measured by invasion and cell migration in malignant CCA cell lines (HuCCA-1 and RMCCA-1). Read More

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Safety and feasibility of resuming bariatric surgery under the cloud of COVID-19.

Ann R Coll Surg Engl 2021 Jul;103(7):524-529

Bedfordshire Hospitals NHS Foundation Trust, UK.

Introduction: Because of the COVID-19 pandemic, numerous bariatric surgical units globally have halted weight loss surgery. Obesity itself has been shown to be a predictor of poor outcome in people infected with the virus. The aim of this study was to report our experience as a high-volume bariatric institution resuming elective weight loss surgery safely amidst emergency admissions of COVID-19-positive patients. Read More

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Neonatal stroke enhances interaction of microglia-derived extracellular vesicles with microglial cells.

Neurobiol Dis 2021 Jun 18:105431. Epub 2021 Jun 18.

Department of Neurology, UCSF, San Francisco, CA, USA. Electronic address:

Microglial cells support brain homeostasis under physiological conditions and modulate brain injury in a context-dependent and brain maturation-dependent manner. Microglial cells protect neonatal brain from acute stroke. While microglial signaling via direct cell-cell interaction and release of variety of molecules is intensely studied, less is known about microglial signaling via release and uptake of extracellular vesicles (EVs). Read More

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A Polylactosamine-Specific Lectin From Adenia Hondala Induces Apoptosis And Necrosis In Human Epithelial Colon Cancer Ht-29 Cells.

Protein Pept Lett 2021 Jun 15. Epub 2021 Jun 15.

Department of Studies in Biochemistry, Karnatak University, Dharwad-580003, India.

Background: Altered expression of N-glycans such as polylactosamine is observed in colon cancer. AHL, a polylactosamine specific lectin from Adenia hondala from a medicinal plant from the Passifloraceae family, has been reported earlier.

Objective: The aim of the present study is to study the interaction of AHL with human colon cancer epithelial HT-29 cells and colon cancer tissues. Read More

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High-speed fluoroscopic imaging for investigation of three-dimensional knee kinematics before and after marathon running.

Gait Posture 2021 Jun 8;88:231-237. Epub 2021 Jun 8.

Key Laboratory of Exercise and Health Sciences of Ministry of Education, School of Kinesiology, Shanghai University of Sport, Shanghai, China. Electronic address:

Background: Knee injuries often occur during or shortly after marathon running, and are linked to altered knee kinematics.

Research Question: The kinematics of healthy knees during pre- and post-marathon running have not been examined with high-speed fluoroscopy. This study aimed to evaluate the effects of marathon running on knee kinematics during walking and running by using a combined high-speed fluoroscopy and MRI technique. Read More

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Doxorubicin Loaded Dextran-coated Superparamagnetic Iron Oxide Nanoparticles with Sustained Release Property: Intracellular Uptake, Pharmacokinetics, and Biodistribution Study.

Curr Pharm Biotechnol 2021 Jun 4. Epub 2021 Jun 4.

National Engineering Research Center for Miniaturized Detection Systems, Northwest University, Xi'an, China.

Background: Due to the short biological half-life and serious side effects (especially for heart and kidney), the application of Doxorubicin (Dox) in clinical therapy is strictly limited. To overcome these shortcomings, a novel sustained release formulation of doxorubicin-loaded dextran-coated superparamagnetic iron oxide nanoparticles (Dox-DSPIONs) was prepared.

Objective: The purpose of this study was to evaluate the intracellular uptake behavior of Dox-DSPIONs and to investigate their pharmacokinetics and biodistribution properties. Read More

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Mitochondrial and transcriptome responses in rat dopaminergic neuronal cells following exposure to the insecticide fipronil.

Neurotoxicology 2021 Jul 25;85:173-185. Epub 2021 May 25.

Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA. Electronic address:

The phenylpyrazole fipronil is an insecticide that inhibits γ -amino-butyric acid (GABA) ionotropic receptors in the central nervous system. Experimental evidence suggests that fipronil acts as a neurotoxin and it is implicated in neurodegenerative diseases; however, the mechanisms of neurotoxicity are not fully elucidated. The objective of this study was to quantify mechanisms of fipronil-induced neurotoxicity in dopamine cells. Read More

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PI3K, mTOR and GSK3 modulate cytokines' production in peripheral leukocyte in temporal lobe epilepsy.

Neurosci Lett 2021 06 9;756:135948. Epub 2021 May 9.

Neuroscience Program, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil; Department of Pharmacology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address:

Introduction: Epilepsy is a common pathological condition that predisposes individuals to seizures, as well as cognitive and emotional dysfunctions. Different studies have demonstrated that inflammation contributes to the pathophysiology of epilepsy. Indeed, seizures change the peripheral inflammatory pattern, which, in turn, could contribute to seizures. Read More

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Compromised right ventricular contractility in an ovine model of heart transplantation following 24 h donor brain stem death.

Pharmacol Res 2021 Jul 24;169:105631. Epub 2021 Apr 24.

Critical Care Research Group, The Prince Charles Hospital, Queensland, Australia; School of Medical Sciences, Griffith University, Queensland, Australia; Prince Charles Hospital Northside Clinical Unit, Faculty of Medicine, University of Queensland, Australia; Faculty of Health, School of Biomedical Sciences, Queensland University of Technology, Australia; Cardiothoracic Surgery and Transplantation, The Alfred Hospital, and Monash University, Melbourne, Australia.

Background: Heart failure is an inexorably progressive disease with a high mortality, for which heart transplantation (HTx) remains the gold standard treatment. Currently, donor hearts are primarily derived from patients following brain stem death (BSD). BSD causes activation of the sympathetic nervous system, increases endothelin levels, and triggers significant inflammation that together with potential myocardial injury associated with the transplant procedure, may affect contractility of the donor heart. Read More

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Endogenous cortisol excess confers a unique lipid signature and metabolic network.

J Mol Med (Berl) 2021 Apr 21. Epub 2021 Apr 21.

Group of Endocrine Disorders, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Carrer Rosselló, 149, 08036, Barcelona, Spain.

Chronic cortisol excess induces several alterations on protein, lipid and carbohydrate metabolism resembling those found in the metabolic syndrome. However, patients exposed to prolonged high levels of cortisol in Cushing syndrome (CS) present exceeding cardiometabolic alterations not reflected by conventional biomarkers. Using 3 ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS) platforms, we aimed to characterise the serum metabolome of 25 patients with active endogenous CS and 25 control subjects matched by propensity score (sex, BMI, diabetes mellitus type 2 (T2D), high blood pressure (HBP) and dyslipidaemia) to search for potential disease-specific biomarkers and pathways associated to the clinical comorbidities. Read More

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Blood pressure monitoring in kidney transplantation: a systematic review on hypertension and target organ damage.

Nephrol Dial Transplant 2021 Mar 25. Epub 2021 Mar 25.

CNR-Institute of Clinical Physiology; Clinical Epidemiology and Physiopathology of Renal Diseases and Hypertension, Reggio Calabria, Italy.

Background: Sparse studies show that ambulatory blood pressure monitoring (ABPM) is superior to office BP (oBP) measurements to predict target organ damage and cardiovascular (CV) events in kidney transplant recipients (KTRs). We performed a systematic review aimed at determining the potential associations between BP recordings by different methods and renal and CV outcomes in this population.

Methods: Major medical databases were searched for studies enrolling adult KTRs undergoing 24h ABPM compared to office or home BP measurements. Read More

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High Fat Diet Dysbiotic Mechanism of Decreased Gingival Blood Flow.

Front Physiol 2021 3;12:625780. Epub 2021 Mar 3.

Department of Physiology, School of Medicine, University of Louisville, Louisville, KY, United States.

The gut microbiome has a very important role in human health and its influence on the development of numerous diseases is well known. In this study, we investigated the effect of high fat diet (HFD) on the onset of dysbiosis, gingival blood flow decreases, and the periodontal matrix remodeling. We established a dysbiosis model (HFD group) and probiotic model by GG (LGG) treatment for 12weeks. Read More

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Mortality Risk Assessment at the Admission in Patient With Proximal Femur Fractures: Electrolytes and Renal Function.

Geriatr Orthop Surg Rehabil 2021 9;12:2151459321991503. Epub 2021 Feb 9.

Department of Orthopaedics and Traumatology (DiChirOnS), University of Palermo, Palermo, Italy.

In patients over 65y.o. who were surgically treated for a hip fracture, electrolytes have not been specifically studied as predictors of mortality. Read More

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February 2021

Food anthocyanins decrease concentrations of TNF-α in older adults with mild cognitive impairment: A randomized, controlled, double blind clinical trial.

Nutr Metab Cardiovasc Dis 2021 03 5;31(3):950-960. Epub 2020 Dec 5.

School of Medicine, Faculty of Science, Medicine and Health, University of Wollongong, Wollongong, NSW, 2522, Australia; Illawarra Health & Medical Research Institute, Wollongong, NSW, 2522, Australia. Electronic address:

Background & Aims: Vascular function, blood pressure and inflammation are involved in the pathogenesis of major chronic diseases, including both cardiovascular disease (CVD) and mild cognitive impairment (MCI). This study investigated the effects of food anthocyanins on microvascular function, 24-h ambulatory blood pressure (ABP) and inflammatory biomarkers in older adults with MCI.

Methods And Results: Thirty-one participants with MCI [19 female, 12 male, mean age 75. Read More

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Chemical composition and phytotoxicity of essential oil from invasive plant, Ambrosia artemisiifolia L.

Ecotoxicol Environ Saf 2021 Mar 16;211:111879. Epub 2021 Jan 16.

Xinjiang University, Resource and Environment Science College, Urumqi 830046, China. Electronic address:

Essential oils have been evaluated as appropriate phytotoxins with mechanisms of action that are different from those of synthetic herbicides applied in weed management activities, but little is known about the effect of Ambrosia artemisiifolia essential oil (EO) on weeds. Here, the chemical composition of A. artemisiifolia EO was analyzed using a Gas Chromatography-Mass Spectrometry system. Read More

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Discrimination between Fresh and Frozen-Thawed Fish Involved in Food Safety and Fraud Protection.

Foods 2020 Dec 18;9(12). Epub 2020 Dec 18.

Department of Health, Animal Science and Food Safety, Università Degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy.

This study aims to discriminate fresh fish from frozen/thawed by identification of the key metabolites that are altered during the freezing/thawing processing. Atlantic salmon ( and bullet tuna () were selected as they are representative of broad consumption, and susceptible to pathogen contamination. Atlantic salmon samples were subjected to the following regimes: -20 °C (24h) and -35 °C (15 h) freezing, then thawed respectively in the blast chiller and in the cold room and analyzed immediately or after 10 days; (2) bullet tuna samples were frozen at -18 °C and thawed after 15, 30 and 90 days. Read More

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December 2020

Anti-Leishmania amazonensis activity of the terpenoid fraction from Eugenia pruniformis leaves.

An Acad Bras Cienc 2020 7;92(4):e20201181. Epub 2020 Dec 7.

Universidade Federal Fluminense, Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Rua Dr. Mário Vianna, 523, Santa Rosa, 24241-000 Niterói, RJ, Brazil.

Leishmaniasis is caused by protozoan parasites belonging to the genus Leishmania and includes cutaneous, mucocutaneous and visceral clinical forms. Drugs currently available for leishmaniasis treatment present high toxicity, and development of parasite resistance. Plants constitute an important source of compounds with leishmanicidal potential. Read More

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December 2020

Endothelin-1 decreases the expression of Ephrin-A and B subtypes in cultured rat astrocytes through ET receptors.

Neurosci Lett 2021 01 3;741:135393. Epub 2020 Dec 3.

Laboratory of Pharmacology, Kobe Pharmaceutical University, 4-19-1 Motoyama-Kita Higashinada, Kobe 668-8558 Japan.

Ephrin family proteins are cell surface molecules that regulate several cellular functions through cell-cell interactions. During nervous tissue repair after injury, the expression of ephrin subtypes in astrocytes is altered, affecting the axonal elongation and migration of neuronal precursors. However, the mechanism regulating the expression of ephrin subtypes in astrocytes has not been investigated. Read More

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January 2021

Effects of ultraviolet radiation to Solea senegalensis during early development.

Sci Total Environ 2021 Apr 14;764:142899. Epub 2020 Oct 14.

CESAM (Centre for Environmental and Marine Studies) & Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

Ultraviolet radiation (UVR) reaching the Earth surface is increasing and scarce information is available regarding effects of this stressor to early life stages of marine vertebrates. Therefore, this work aims to study the effects of UVR exposure during early development stages of the flatfish Solea senegalensis. Firstly, fish were exposed to UVR (six daily doses between 3. Read More

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Health risks of chlorothalonil, carbendazim, prochloraz, their binary and ternary mixtures on embryonic and larval zebrafish based on metabolomics analysis.

J Hazard Mater 2021 02 10;404(Pt B):124240. Epub 2020 Oct 10.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address:

Chlorothalonil (CTL), carbendazim (CBZ), and prochloraz (PCZ) are fungicides widely used in many countries. The use of these fungicides raises concerns because they are often applied together or used in the same agricultural area. However, the toxicity of these fungicides or mixtures, especially to aquatic organisms, has received limited attention. Read More

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February 2021

Exposure to nanoceria impacts larval survival, life history traits and fecundity of Aedes aegypti.

PLoS Negl Trop Dis 2020 09 25;14(9):e0008654. Epub 2020 Sep 25.

University of Central Florida College of Medicine, Department of Internal Medicine, Orlando, Florida, United States of America.

Effectively controlling vector mosquito populations while avoiding the development of resistance remains a prevalent and increasing obstacle to integrated vector management. Although, metallic nanoparticles have previously shown promise in controlling larval populations via mechanisms which are less likely to spur resistance, the impacts of such particles on life history traits and fecundity of mosquitoes are understudied. Herein, we investigate the chemically well-defined cerium oxide nanoparticles (CNPs) and silver-doped nanoceria (AgCNPs) for larvicidal potential and effects on life history traits and fecundity of Aedes (Ae. Read More

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September 2020

A novel time-dimension and circadian rhythm-dependent strategy for pharmacodynamic evaluation of Uncaria in the regulation of neurotransmitter circadian metabolic homeostasis in spontaneously hypertensive rats.

Biomed Pharmacother 2020 Nov 9;131:110704. Epub 2020 Sep 9.

Experiment Center, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China; Key Laboratory of Traditional Chinese Medicine Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan, 250355, PR China; Shandong Provincial Key Laboratory of Traditional Chinese Medicine for Basic Research, Shandong University of Traditional Chinese Medicine, Jinan, 250355, PR China. Electronic address:

In the present study, we aimed to use metabolomics platforms to examine circadian-regulated neurotransmitters across a 24-h day and the effects of Uncaria administration on daily rhythmicity in order to establish a strategy for evaluating the spatiotemporal efficacy evaluation strategy of Uncaria. By using targeted ultrahigh performance liquid chromatography-mass spectrometry metabolomics, we quantified 32 neurotransmitter metabolites every 4 h over 24 h. To assess 24-h metabolite rhythmicity, we performed cosinor analysis. Read More

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November 2020

Effect of an Intracerebroventricular Injection of Aggregated Beta-amyloid (1-42) on Daily Rhythms of Oxidative Stress Parameters in the Prefrontal Cortex.

Neuroscience 2021 03 19;458:99-107. Epub 2020 Aug 19.

Laboratorio de Cronobiología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis Instituto Multidisciplinario de Investigaciones Biológicas de San Luis (IMIBIO-SL), CONICET, Ejército de Los Andes 950, CP D5700HHW San Luis, Argentina. Electronic address:

Accumulation of amyloid peptides in the brain plays a key role in the pathogenesis of Alzheimer's disease (AD). Aggregated beta-amyloid (Aβ) peptide increases intracellular reactive oxygen species associated to a deficient antioxidant defense system. Prefrontal cortex plays a key role in memory and learning and is especially susceptible to oxidative stress. Read More

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Endocrine-disrupting chemicals rapidly affect intercellular signaling in Leydig cells.

Toxicol Appl Pharmacol 2020 10 31;404:115177. Epub 2020 Jul 31.

RECETOX, Faculty of Science, Masaryk University, Kamenice 753/5, building A29, 625 00 Brno, Czech Republic. Electronic address:

A decline in male fertility possibly caused by environmental contaminants, namely endocrine-disrupting chemicals (EDCs), is a topic of public concern and scientific interest. This study addresses a specific role of testicular gap junctional intercellular communication (GJIC) between adjacent prepubertal Leydig cells in endocrine disruption and male reproductive toxicity. Organochlorine pesticides (lindane, methoxychlor, DDT), industrial chemicals (PCB153, bisphenol A, nonylphenol and octylphenol) as well as personal care product components (triclosan, triclocarban) rapidly dysregulated GJIC in murine Leydig TM3 cells. Read More

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October 2020

Differential effects of spinal cord transection on glycinergic and GABAergic synaptic signaling in sub-lesional lumbar motoneurons.

J Chem Neuroanat 2021 Apr 9;113:101847. Epub 2020 Jul 9.

Institut de Neurosciences de la Timone, UMR 7289, CNRS and Aix Marseille Université, Campus Santé Timone, 13385, Marseille, France.

This review takes stock on the impact of complete spinal cord transection (SCT) on the plasticity of inhibitory synaptic transmission on sub-lesional lumbar motoneurons (MNs), differentiating between studies in neonate and adult rats. After neonatal SCT, normal maturational up-regulation of glycine receptors was observed. On the other hand, the developmental downregulation of the GABAA receptors, as well as the up-regulation of the co-transporter KCC2 were prevented, but not the normal decrease of NKCC1. Read More

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Hemodynamic Effects of a Multidisciplinary Geriatric Rehabilitation Program.

Clin Interv Aging 2020 30;15:1035-1043. Epub 2020 Jun 30.

University Hospital Marien Hospital Herne, Department of Internal Medicine I, Ruhr-University Bochum, Bochum, Germany.

Background: Geriatric rehabilitation programs primarily aim at an increase of mobility and functional autonomy of the elderly. The cardiovascular effects of these programs, however, remain elusive. Since regular physical exercise is associated with numerous beneficial cardiovascular effects including a reduction of blood pressure (BP), the present prospective study investigates the hemodynamic effects of a representative standardized rehabilitation program. Read More

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October 2020

In vitro effects of boric acid on human liver hepatoma cell line (HepG2) at the half-maximal inhibitory concentration.

J Trace Elem Med Biol 2020 Dec 30;62:126573. Epub 2020 May 30.

Middle East Technical University, Graduate School of Arts and Sciences, Department of Biological Sciences, Ankara, Turkey. Electronic address:

Background: Boron is a prominent part of the human diet and one of the essential trace elements for humans. Dietary boron is mostly transformed into boric acid within the body and has been associated with desirable health outcomes. Non-dietary resources of boron, such as boron-based drugs and occupational exposure, might lead to excessive boron levels in the blood and provoke health adversities. Read More

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December 2020

Brown adipose tissue recruitment in a rodent model of severe burns.

Burns 2020 May 19. Epub 2020 May 19.

Department of Surgery, University of Texas Medical Branch, Galveston, TX, United States; Shriners Hospitals for Children, Galveston, TX, United States. Electronic address:

Background: Severe burns results in a prolonged hypermetabolic response. Brown adipose tissue (BAT), abundant in uncoupling protein 1 (UCP1), plays a key role in non-shivering thermogenesis. We set out to determine if BAT is recruited in response to severe burns. Read More

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Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice.

PLoS Negl Trop Dis 2020 06 1;14(6):e0008379. Epub 2020 Jun 1.

Laboratory of Pathophysiology, Butantan Institute, São Paulo, São Paulo, Brazil.

Few studies have addressed gene expression of hemostasis-related factors during acute thrombo-hemorrhagic diseases. Bites by the lanced-headed viper Bothrops jaracaca induce rapid hemostatic disturbances in victims, leading to systemic bleedings, thrombocytopenia and consumption coagulopathy. Although circulating levels of coagulation factors recover rapidly after administration of specific antivenom therapy, it is unclear if B. Read More

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Combined Danio rerio embryo morbidity, mortality and photomotor response assay: A tool for developmental risk assessment from chronic cyanoHAB exposure.

Sci Total Environ 2019 Dec 31;697:134210. Epub 2019 Aug 31.

Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, 97331, USA; Sinnhuber Aquatic Research Laboratory, Oregon State University, 28645 East Highway 34, Corvallis, OR 97333, USA.

Freshwater harmful algal blooms produce a broad array of bioactive compounds, with variable polarity. Acute exposure to cyanotoxins can impact the liver, nervous system, gastrointestinal tract, skin, and immune function. Increasing evidence suggests chronic effects from low-level exposures of cyanotoxins and other associated bioactive metabolites of cyanobacterial origin. Read More

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December 2019