444 results match your criteria alpha-amylase short


Fatigue, Stress, and Performance during Alternating Physical and Cognitive Tasks-Effects of the Temporal Pattern of Alternations.

Ann Work Expo Health 2021 Jul 6. Epub 2021 Jul 6.

Department of Occupational Health Sciences and Psychology, Faculty of Health and Occupational Studies, University of Gävle, Kungsbäcksvägen 47, 801 76 Gävle, Sweden.

In occupational life, performing cognitive work tasks in between fatiguing physical work tasks may allow recovery and reduce stress without losing productive working time. The temporal pattern of such alternations is likely a determinant of the recovery effect, influencing both stress and fatigue; the difficulty of the cognitive task (CT) would also be a likely determinant. The aim of this study was to determine to what extent the temporal pattern of alternations between a repetitive physical task and a CT of different difficulties influenced perceived fatigability, performance fatigability, stress-related outcomes, and performance. Read More

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Ameliorative effect of Annona reticulata L. leaf extract on antihyperglycemic activity and its hepato-renal protective potential in streptozotocin induced diabetic rats.

J Ayurveda Integr Med 2021 Jun 16. Epub 2021 Jun 16.

Department of Biotechnology, Dravidian University, Kuppam, 517 426, A.P, India. Electronic address:

Background: Annona reticulata L. is a traditionally important plant due to its versatile source of medicine and industrial products. It is used to treat cardiac problems, wound healing, diabetes, ulcers and bacterial infections. Read More

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Investigation of starch functionality and digestibility in white wheat bread produced from a recipe containing added maltogenic amylase or amylomaltase.

Food Chem 2021 Nov 29;362:130203. Epub 2021 May 29.

Laboratory of Food Chemistry and Biochemistry and Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven, Kasteelpark Arenberg 20, B-3001 Leuven, Belgium. Electronic address:

In the crumb of fresh white wheat bread, starch is fully gelatinized. Its molecular and three-dimensional structure are major factors limiting the rate of its digestion. The aim of this study was to in situ modify starch during bread making with starch-modifying enzymes (maltogenic amylase and amylomaltase) and to investigate the impact thereof on bread characteristics, starch retrogradation and digestibility. Read More

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November 2021

Effects of oral lubrication on satiety, satiation and salivary biomarkers in model foods: A pilot study.

Appetite 2021 10 26;165:105427. Epub 2021 May 26.

Food Colloids and Bioprocessing Group, School of Food Science and Nutrition, University of Leeds, Leeds, LS2 9JT, United Kingdom. Electronic address:

With a dramatic increase in overweight and population with obesity over the last decades, there is an imminent need to tackle this issue using novel strategies. Addressing obesity issues by generating satiety in food to reduce energy intake has been one of those prominent strategies and often textural interventions have been used to generate satiety, specifically in short-term trials. This study aimed to investigate the role of preloads varying in their oral lubricating properties on appetite sensations, food intake, salivary friction and concentration of salivary biomarkers (proteins, α-amylase and mucins) in collected human saliva (n = 17 healthy participants). Read More

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October 2021

Preferential growth stimulation of probiotic bacteria by galactan exopolysaccharide from Weissella confusa KR780676.

Food Res Int 2021 05 23;143:110333. Epub 2021 Mar 23.

Department of Food Science and Technology, Pondicherry University, Pondicherry 605014, India. Electronic address:

Many microbial exopolysaccharides (EPS) are reported to have prebiotic properties. This study reports the prebiotic properties of a galactan exopolysaccharide produced by Weissella confusa KR780676 of fermented food origin. We have reported potential techno-functional properties of this EPS earlier. Read More

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Physiological Characteristics and Anti-Diabetic Effect of KI62.

Food Sci Anim Resour 2021 Mar 1;41(2):274-287. Epub 2021 Mar 1.

Korea Food Research Institute, Wanju 55365, Korea.

The purpose of this study is to examine the physiological characteristics and anti-diabetic effects of KI62. The α-amylase and α-glucosidase inhibitory activity of KI62 was 94.86±3. Read More

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Draft nuclear genome and complete mitogenome of the Mediterranean corn borer, Sesamia nonagrioides, a major pest of maize.

G3 (Bethesda) 2021 May 7. Epub 2021 May 7.

Université Paris-Saclay, CNRS, IRD, UMR Évolution, Génomes, Comportement et Écologie, 91198, Gif-sur-Yvette, France.

The Mediterranean corn borer (Sesamia nonagrioides, Noctuidae, Lepidoptera) is a major pest of maize in Europe and Africa. Here, we report an assembly of the nuclear and mitochondrial genome of a pool of inbred males and females third instar larvae, based on short- and long-read sequencing. The complete mitochondrial genome is 15,330 bp and contains all expected 13 and 24 protein-coding and RNA genes, respectively. Read More

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Salivary Protein Profile and Food Intake: A Dietary Pattern Analysis.

J Nutr Metab 2021 15;2021:6629951. Epub 2021 Apr 15.

MED (Mediterranean Institute for Agriculture, Environment and Development), Institute for Advanced Studies and Research (IIFA)IIFA-Instituto de Investigação e Formação Avançada, University of Évora, Évora, Portugal.

Saliva research has gained interest due to its potential as a source of biomarkers. One of the factors inducing changes in saliva, in the short term, is food intake, and evidence exist about changes in salivary proteome induced by some food components. Since this topic of research is in its early stages, it was hypothesized that saliva protein composition could be associated with different levels of adherence to dietary patterns that contain higher amounts of plant products. Read More

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How Different Snacks Produce a Distinct Effect in Salivary Protein Composition.

Molecules 2021 Apr 21;26(9). Epub 2021 Apr 21.

Mediterranean Institute for Agricultural, Environmental and Development, University of Évora, 7002-556 Évora, Portugal.

Saliva secretion changes in response to different stimulation. Studies performed in animals and humans suggest that dietary constituents may influence saliva composition, although the dynamics of these changes, and how they are specific for each type of food, are little known. The objective of the present study was to access the short-term effects of different foods in salivation and salivary protein composition. Read More

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Biochemical and synergistic properties of a novel alpha-amylase from Chinese nong-flavor Daqu.

Microb Cell Fact 2021 Apr 7;20(1):80. Epub 2021 Apr 7.

CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, No. 9 Section 4, Renmin Nan Road, Chengdu, 610041, Sichuan, People's Republic of China.

Background: Daqu is the most important fermentation starter for Chinese liquor, with large number of microbes and enzymes being openly enriched in the Daqu system over thousands of years. However, only a few enzymes have been analyzed with crude protein for total liquefying power and saccharifying power of Daqu. Therefore, the complex enzymatic system present in Daqu has not been completely characterized. Read More

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Physiological and Perceptual Recovery-Stress Responses to an Elite Netball Tournament.

Int J Sports Physiol Perform 2021 Mar 25:1-10. Epub 2021 Mar 25.

Purpose: To investigate and explore the relationships between physiological and perceptual recovery and stress responses to elite netball tournament workloads.

Methods: Nine elite female netballers were observed across a 3-day (T1-3), 4-match tournament. Participants provided salivary samples for cortisol and alpha-amylase analysis, completed the Short Recovery Stress Scale (SRSS), and reported session ratings of perceived exertion. Read More

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Functionality of Djulis () By-Products and In Vivo Anti-Diabetes Effect in Type 2 Diabetes Mellitus Patients.

Biology (Basel) 2021 Feb 17;10(2). Epub 2021 Feb 17.

Department of Medicinal Botanical and Health Applications, Da-Yeh University, No. 168, University Rd, Dacun, Changhua 51591, Taiwan.

Djulis ( Koidz.) is a species of cereal grain native to Taiwan. It is rich in dietary fibre and antioxidants and therefore reputed to relieve constipation, suppress inflammation, and lower blood glucose. Read More

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February 2021

Modulation of gelatinized wheat starch digestion and fermentation profiles by young apple polyphenols in vitro.

Food Funct 2021 Mar 4;12(5):1983-1995. Epub 2021 Feb 4.

College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi 710119, P. R. China.

To evaluate the effect of young apple polyphenols (YAP) on starch digestion and gut microbiota, complexes of native wheat starch (NWS) with YAP, and their main components chlorogenic acid (CA) and phlorizin (P) were fabricated and gelatinized. Through XRD and FTIR analysis, it was found that the partial crystalline structure of NWS was destroyed during gelatinization, and the addition of P decreased the extent of destruction. Then, the gelatinized starchy samples were subjected to in vitro digestion. Read More

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Effects of glucose intake on stress reactivity in young, healthy men.

Psychoneuroendocrinology 2021 Apr 18;126:105062. Epub 2020 Nov 18.

Department of Biological and Clinical Psychology, University of Trier, D-54290, Trier, Germany. Electronic address:

The psychobiological stress response has a broad impact on energy metabolism, while the availability of energy may, in turn, affect the stress response. Specifically, a reduced cortisol response has been found after 8-11 hours of fasting, while glucose intake has led to an increase in cortisol reactivity. We compared the effects of standardized glucose or artificial sweetener drinks, as well as water, ingested prior to a physical (cold pressor test, CPT) or a psychosocial stressor (Trier Social Stress Test, TSST) after four hours of fasting. Read More

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Hybrid QconCAT-Based Targeted Absolute and Data-Independent Acquisition-Based Label-Free Quantification Enables In-Depth Proteomic Characterization of Wheat Amylase/Trypsin Inhibitor Extracts.

J Proteome Res 2021 03 28;20(3):1544-1557. Epub 2021 Jan 28.

Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstr. 1, 55131 Mainz, Germany.

Wheat amylase/trypsin inhibitors (ATIs) have gained significant relevance as inducers of intestinal and extra-intestinal inflammation. In this study, we present a novel hybrid data-independent acquisition (DIA) liquid chromatography-mass spectrometry (LC-MS) approach, combining QconCAT technology with short microflow LC gradients and DIA and apply the method toward the quantitative proteome analysis of ATI extracts. The presented method is fast, robust, and reproducible and provides precise QconCAT-based absolute quantification of major ATI proteins while simultaneously quantifying the proteome by label-free quantification (LFQ). Read More

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Machine Learning-Based Amino Acid Substitution of Short Peptides: Acquisition of Peptides with Enhanced Inhibitory Activities against α-Amylase and α-Glucosidase.

ACS Biomater Sci Eng 2020 11 2;6(11):6117-6125. Epub 2020 Oct 2.

Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.

We developed a method for efficiently activating functional peptides with a large structural contribution using the peptide-searching method with machine learning. The physicochemical properties of the amino acids were employed as variables. As a model peptide, we used GHWYYRCW, which is a functional peptide that inhibits α-amylase derived from human pancreatic juice. Read More

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November 2020

A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation.

Carbohydr Polym 2021 Mar 13;255:117503. Epub 2020 Dec 13.

Department of Molecular Sciences, Swedish University of Agricultural Sciences, Box 7015, SE-750 07, Uppsala, Sweden. Electronic address:

To determine the internal structure of barley starch without amylopectin isolation, whole starch was hydrolyzed using β-amylase to remove the linear amylose and obtain β-limit dextrins (β-LDs). The β-LDs were treated with extensive α-amylase to prepare α-limit dextrins (α-LDs), and the α-LDs were further hydrolyzed with β-amylase into building blocks. The chain-length distribution of β-LD and building block composition were analyzed by size-exclusion chromatography and anion-exchange chromatography. Read More

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In Vitro Alpha-Amylase and Alpha-Glucosidase Inhibitory Activity and In Vivo Antidiabetic Activity of L. Foliar Extract.

Molecules 2021 Jan 8;26(2). Epub 2021 Jan 8.

Laboratory of Biotechnology, Environment, Agrifood, and Health, University of Sidi Mohamed Ben Abdellah, FSDM-Fez, Fez 30003, Morocco.

L. is a wild perennial woody plant used by the local population for diverse therapeutic purposes. This work aims to study for the first time the potential inhibitory effect of this plant hydroethanolic extract on α-amylase and α-glucosidase activities using in vitro methods and its antidiabetic and antihyperglycemic activities using alloxan-induced diabetic mice as a model for experimental diabetes. Read More

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January 2021

Effects of gender and personality on everyday moral decision-making after acute stress exposure.

Psychoneuroendocrinology 2021 02 1;124:105084. Epub 2020 Dec 1.

Department of Psychology, University of Regensburg, 93053 Regensburg, Germany. Electronic address:

Exposure to acute psychosocial stress has been shown to affect moral decision-making, though little is known about potential gender differences or effects of personality. In two within-subjects design studies, 179 healthy men and women (N = 99 in Study 1, N = 80 in Study 2) were exposed to the Trier Social Stress Test (TSST) and a non-stress control condition (resting period) on two testing days in random order. After stress/resting, moral decision-making was assessed by the Everyday Moral Conflict Situations (EMCS) Scale (Singer et al. Read More

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February 2021

Identification of a Candidate Starch Utilizing Strain of from Bovine Rumen.

Microorganisms 2020 Dec 16;8(12). Epub 2020 Dec 16.

Department of Animal Science, South Dakota State University, Animal Science Complex, Box 2170, Brookings, SD 57007, USA.

The inclusion of starch-rich feedstuffs, a common practice in intensive ruminant livestock production systems, can result in ruminal acidosis, a condition that can severely impact animal performance and health. One of the main causes of acidosis is the rapid accumulation of ruminal short chain fatty acids (SCFAs) resulting from the microbial digestion of starch. A greater understanding of ruminal bacterial amylolytic activities is therefore critical to improving mitigation of acidosis. Read More

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December 2020

Positive and Negative Post Performance-Related Thoughts Predict Daily Cortisol Output in University Music Students.

Front Psychol 2020 13;11:585875. Epub 2020 Nov 13.

Center for Primary Care and Public Health, University of Lausanne, Lausanne, Switzerland.

Psychophysiological research on music performance has focused on musicians' short-term affective, cognitive, and physiological responses. Much less attention has been devoted to the investigation of musicians' psychophysiological activity beyond the performance situation. Musicians report having both positive and negative performance-related thoughts (e. Read More

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November 2020

Short-term Cudrania tricuspidata fruit vinegar administration attenuates obesity in high-fat diet-fed mice by improving fat accumulation and metabolic parameters.

Sci Rep 2020 12 3;10(1):21102. Epub 2020 Dec 3.

Department of Food Science and Biotechnology, Gwangju University, Gwangju, 503-703, Republic of Korea.

Previous studies have suggested that vinegar intake can help to reduce body fat and hyperglycemia. Therefore, this study aimed to evaluate the anti-obesity efficacy of vinegar fermented using Cudrania tricuspidata fruits (CTFV) and its main phenolic constituents and to analyze its molecular mechanism and changes in obesity-related metabolizing enzymatic activities. We found that HFD significantly caused hepatic steatosis; increases in body fats, feed efficiency, liver mass, lipids, insulin, oxidative parameters, cardiovascular-associated risk indices, lipase and α-amylase activities, whereas CTFV efficaciously attenuated HFD-induced oxidant stress, fat accumulation, obesity-related enzymatic activity, and the activation or reduction of obesity-related molecular reactions via improving metabolic parameters including phosphorylated insulin receptor substrate 1, protein tyrosine phosphatase 1B, phosphorylated phosphoinositide 3-kinase/protein kinase B, phosphorylated mitogen-activated protein kinases, sterol regulatory element-binding protein 1c, CCAAT/enhancer-binding protein, and fatty acid synthase; and decreases in adiponectin receptor 1, leptin receptor, adenosine monophosphate-activated protein kinase, acetyl-CoA carboxylase, and peroxisome proliferator-activated receptor, subsequently ameliorating HFD-induced obesity. Read More

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December 2020

Highly branched corn starch: Preparation, encapsulation, and release of ascorbic acid.

Food Chem 2021 May 3;343:128485. Epub 2020 Nov 3.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China. Electronic address:

The aim of this study was to prepare a supporting carrier, namely highly branched corn starch (HBCS), and to investigate its encapsulation property with ascorbic acid (AA). High amylose corn starch was converted into HBCS via dual enzymatic modification by successively using α-amylase and glycogen branching enzyme. The results showed that the ratio of α-1, 6 linkage of HBCS increased by 1. Read More

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Salivary markers of stress system activation and social withdrawal in humans.

J Psychiatr Res 2021 04 22;136:435-443. Epub 2020 Oct 22.

Department of Psychiatry, Leiden University Medical Center (LUMC), the Netherlands.

Social withdrawal is an early and common feature of psychiatric disorders. Hypothalamic-pituitary-adrenal (HPA)-axis activation through increased salivary cortisol (sC) and sympathetic activation through increased salivary alpha-amylase (sAA) may play a role. We aimed to study whether the link between increased sC and sAA on the one hand and depression on the other hand is mediated by social withdrawal. Read More

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Generation of short-chained granular corn starch by maltogenic α-amylase and transglucosidase treatment.

Carbohydr Polym 2021 Jan 7;251:117056. Epub 2020 Sep 7.

Department of Plant and Environmental Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark. Electronic address:

We describe a method for permitting efficient modification by transglucosidase (TGA), from glycoside hydrolase family 31 (GH31), sequentially after the pre-treatment by maltogenic α-amylases (MA) from GH13. TGA treatment without MA pre-treatment had negligible effects on native starch, while TGA treatment with MA pre-treatment resulted in porous granules and increased permeability to enzymes. MA→TGA treatments lead to decreased molecular size of amylopectin molecules, increased α-1,6 branching, and increased amounts of amylopectin chains with the degree of polymerization (DP)<10 and decreased amounts of DP 10-28 after debranching. Read More

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January 2021

1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese.

Poult Sci 2020 Nov 29;99(11):5858-5866. Epub 2020 Aug 29.

School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, People's Republic of China; Institute of Sericulture, Chinese Academy of Agricultural Sciences, Zhenjiang 212003, People's Republic of China. Electronic address:

This study was aimed to investigate whether 1-deoxynojirimycin (DNJ) affects the digestion system of young geese and assess whether mulberry leaf, which contains this substance, has disadvantages that compromise its value as poultry feed. One hundred and twenty-eight 12-day-old male Wanxi white geese were randomly assigned into 4 treatment groups. The control group was fed an ordinary diet without DNJ. Read More

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November 2020

Galactofucan from Laminaria japonica is not degraded by the human digestive system but inhibits pancreatic lipase and modifies the intestinal microbiota.

Int J Biol Macromol 2021 Jan 29;166:611-620. Epub 2020 Oct 29.

Collaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian 116034, China. Electronic address:

The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study. Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized. Read More

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January 2021

Evaluation of the impact of heat on wheat dormancy, late maturity α-amylase and grain size under controlled conditions in diverse germplasm.

Sci Rep 2020 10 20;10(1):17800. Epub 2020 Oct 20.

CSIRO Agriculture and Food, Black Mountain Science and Innovation Park, Canberra, ACT, Australia.

In the Australian wheat belts, short episodes of high temperatures or hot spells during grain filling are becoming increasingly common and have an enormous impact on yield and quality, bringing multi-billion losses annually. This problem will become recurrent under the climate change scenario that forecast increasing extreme temperatures, but so far, no systematic analysis of the resistance to hot spells has yet been performed in a diverse genetic background. We developed a protocol to study the effects of heat on three important traits: grain size, grain dormancy and the presence of Late Maturity α-Amylase (LMA), and we validated it by analysing the phenotypes of 28 genetically diverse wheat landraces and exploring the potential variability existing in the responses to hot spells. Read More

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October 2020

Inhibition of starch digestion by flavonoids: Role of flavonoid-amylase binding kinetics.

Food Chem 2021 Mar 1;341(Pt 2):128256. Epub 2020 Oct 1.

School of Chemistry and Biomolecular Sciences, Faculty of Sciences, University of Ottawa, Canada; School of Nutrition Sciences, Faculty of Health Sciences, University of Ottawa, Canada. Electronic address:

In this study, kinetics of binding between α-amylase and green tea flavonoids were investigated by fluorescence quenching (FQ). Their effect on α-amylase inhibition was evaluated. Whereas epicatechin (EC) and epigallocatechin (EGC) exhibited slow binding kinetics (in the order of minutes), epicatechin gallate (ECG) and epigallocatechin gallate (ECGC) exhibited very rapid binding (in the order of seconds) with Human Salivary α-amylase (HSA) and Porcine Pancreatic α-amylase (PPA). Read More

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Toxoplasma gondii α-amylase deletion mutant is a promising vaccine against acute and chronic toxoplasmosis.

Microb Biotechnol 2020 11 22;13(6):2057-2069. Epub 2020 Sep 22.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Individuals with inhibited immunity may develop lethal toxoplasmosis; thus, a safe and effective vaccine is urged to be developed. Toxoplasma gondii (T. gondii) α-amylase (α-AMY) is one of the enzymes responsible for starch digestion. Read More

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November 2020