13,488 results match your criteria allows reliable


On-site processing of single chromosomal DNA molecules using optically driven microtools on a microfluidic workbench.

Sci Rep 2021 Apr 12;11(1):7961. Epub 2021 Apr 12.

Department of Intelligent Mechanical Systems Engineering, Kagawa University, Takamatsu, 761-0396, Japan.

We developed optically driven microtools for processing single biomolecules using a microfluidic workbench composed of a microfluidic platform that functions under an optical microscope. The optically driven microtools have enzymes immobilized on their surfaces, which catalyze chemical reactions for molecular processing in a confined space. Optical manipulation of the microtools enables them to be integrated with a microfluidic device for controlling the position, orientation, shape of the target sample. Read More

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Simple and reliable protocol for identifying talented junior players in team sports using small-sided games.

Scand J Med Sci Sports 2021 Apr 12. Epub 2021 Apr 12.

School of Biological Sciences, The University of Queensland, St Lucia, QLD, 4072, Australia.

We designed and tested a protocol for measuring the performance of individuals in small-sided soccer games. We tested our protocol on three different groups of youth players from elite Brazilian football academies. Players in each group played a series of 3v3 games, in which individuals were randomly assigned into new teams and against new opponents for each game. Read More

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The epicondylar ratio can be reliably determined in both computed tomography and X-ray.

Arch Orthop Trauma Surg 2021 Apr 11. Epub 2021 Apr 11.

Department of Orthopaedic Surgery, University of Ulm, Ober Eselsberg 45, 89081, Ulm, Germany.

Purpose: One of the key factors to the successful revision of total knee arthroplasty (rTKA) is the reconstruction of the joint line, which can be determined using the epicondylar ratio (ER). The measurement is established in X-ray and MRI. However, it is not known whether computed tomography (CT) allows a more reliable determination. Read More

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A rapid method for the detection and quantification of legacy and emerging per- and polyfluoroalkyl substances (PFAS) in bird feathers using UPLC-MS/MS.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Mar 10;1172:122653. Epub 2021 Mar 10.

Integrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Electronic address:

The bioaccumulation and toxicity of per- and polyfluoroalkyl substances (PFAS) have raised scientific and public concern in recent decades, leading to regulatory measures for some PFAS (e.g. perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA)). Read More

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Implementation of an in-house real-time reverse transcription-PCR assay for the rapid detection of the SARS-CoV-2 Marseille-4 variant.

J Clin Virol 2021 Mar 31;139:104814. Epub 2021 Mar 31.

IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005, Marseille, France; Aix-Marseille Univ., Institut de Recherche pour le Développement (IRD), Assistance Publique - Hôpitaux de Marseille (AP-HM), Microbes Evolution Phylogeny and Infections (MEPHI), 27 Boulevard Jean Moulin, 13005, Marseille, France. Electronic address:

Introduction: The SARS-CoV-2 pandemic has been associated with the occurrence since summer 2020 of several viral variants that overlapped or succeeded each other in time. Those of current concern harbor mutations within the spike receptor binding domain (RBD) that may be associated with viral escape to immune responses. In our geographical area a viral variant we named Marseille-4 harbors a S477 N substitution in this RBD. Read More

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A novel universal algorithm for filament network tracing and cytoskeleton analysis.

FASEB J 2021 May;35(5):e21582

Department of Physics, Saarland University, Saarbruecken, Germany.

The rapid development of advanced microscopy techniques over recent decades has significantly increased the quality of imaging and our understanding of subcellular structures, such as the organization of the filaments of the cytoskeleton using fluorescence and electron microscopy. However, these recent improvements in imaging techniques have not been matched by similar development of techniques for computational analysis of the images of filament networks that can now be obtained. Hence, for a wide range of applications, reliable computational analysis of such two-dimensional methods remains challenging. Read More

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[Osseous variations in radiological diagnostics of the wrist].

Radiologe 2021 Apr 8. Epub 2021 Apr 8.

Diagnostische und interventionelle Radiologie, Universitätsklinikum Tübingen, BG Unfallklinik Tübingen, Eberhard-Karls-Universität, Tübingen, Deutschland.

Clinical/methodical Issue: The carpal joint is one of the most complex joints in the body comprising multiple bones that allow flexibility while simultaneously providing stability. Variations in osseous structures that may be either cause or result of pathological changes may make radiological reporting challenging. Only the knowledge of important osseous variations allows a reliable assessment of carpal imaging studies. Read More

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Basics for surgeons about the immunohistochemistry role in pancreatic NETs diagnosis.

Eur Rev Med Pharmacol Sci 2021 Mar;25(6):2548-2553

Department of General Surgery, Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy.

Objective: Pancreatic neuroendocrine tumors (pNETs) are neuroendocrine tumors primarily found in the pancreas and upper small intestine. There are ten different pNETs: nine of these are associated with a specific functional syndrome, while one is not associated with a specific hormonal syndrome, and it is called non-functional. Up to 90% of pNETs are classified as non-functional. Read More

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Bioanalytical screening of low levels of dioxins and dioxin-like PCBs in pig meat (pork) for checking compliance with EU maximum and action levels using highly sensitive "third generation" recombinant H4L7.5c2 rat hepatoma cells.

Environ Sci Eur 2021 18;33(1):33. Epub 2021 Mar 18.

Department of Environmental Toxicology, University of California Davis (UCD), One Shields Avenue, Davis, CA 95616 USA.

Background: Low maximum and action levels set by the European Union for polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) in pig meat (pork) have led to a demand for reliable and cost-effective bioanalytical screening methods implemented upstream of gas chromatography/high-resolution mass spectrometry confirmatory technology, that can detect low levels of contamination in EU-regulated foods with quick turn-around times.

Results: Based on the Chemically Activated LUciferase gene eXpression (CALUX) bioassay, extraction and clean-up steps were optimized for recovery and reproducibility within working ranges significantly lower than in current bioassays. A highly sensitive "3rd generation" recombinant rat hepatoma cell line (H4L7. Read More

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Dual-Illumination Ultrasound/ Photoacoustic System for Cervical Cancer imaging.

IEEE Photonics J 2021 Feb 14;13(1). Epub 2020 Dec 14.

Department of Biomedical Engineering, Wayne State University, Detroit, MI, USA.

Early stage cancer detection technologies can provide functional information and potentially decrease the mortality rate caused by cervical cancer. In our previous work, a miniaturized ultrasound and photoacoustic endoscopic system has been developed to image the cervical tissue through the cervical canal to fulfills the need for a safe, low-cost, and high-resolution functional diagnostic system. However, the miniaturized size of endoscope and American National Standards Institute safety limits cause constraints of using high-intensity illumination during imaging. Read More

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February 2021

Image-guided microwave ablation of hepatocellular carcinoma (≤5.0 cm): is MR guidance more effective than CT guidance?

BMC Cancer 2021 Apr 7;21(1):366. Epub 2021 Apr 7.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou City, 450000, Henan Province, China.

Background: Given their widespread availability and relatively low cost, percutaneous thermal ablation is commonly performed under the guidance of computed tomography (CT) or ultrasound (US). However, such imaging modalities may be restricted due to insufficient image contrast and limited tumor visibility, which results in imperfect intraoperative treatment or an increased risk of damage to critical anatomical structures. Currently, magnetic resonance (MR) guidance has been proven to be a possible solution to overcome the above shortcomings, as it provides more reliable visualization of the target tumor and allows for multiplanar capabilities, making it the modality of choice. Read More

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How I treat primary ITP in adult patients who are unresponsive to or dependent on corticosteroid treatment.

Blood 2021 Apr 7. Epub 2021 Apr 7.

Massachusetts General Hospital, Boston, Mississippi, United States.

Approximately 80% of adult patients with immune thrombocytopenia (ITP) fail or become dependent on corticosteroids and require second-line therapy. Several new and effective therapies have been introduced during the last decade and our understanding of disease burden and its effect on quality of life has expanded. It is now recommended that splenectomy, the standard second-line therapy for decades, be delayed for at least 12-24 months allowing more patients to achieve remission on medical therapies before considering surgery. Read More

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Transfer Learning from Simulation to Experimental Data: NMR Chemical Shift Predictions.

J Phys Chem Lett 2021 Apr 7:3662-3668. Epub 2021 Apr 7.

Division of National Supercomputing, Korea Institute of Science and Technology Information, 245 Daehak-Ro, Yuseong-Gu, Daejeon 34141, Republic of Korea.

An accurate prediction of chemical shifts (δ) to elucidate molecular structures has been a challenging problem. Recently, noble machine learning architectures achieve accurate prediction performance, but the difficulty of building a huge chemical database limits the applicability of machine learning approaches. In this work, we demonstrate that the prior knowledge gained from the simulation database is successfully transferred into the problem of predicting an experimentally measured δ. Read More

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Statistical study of Khibiny Alkaline Massif (Kola Peninsula) groundwater quality with respect to elevated aluminum concentrations.

Environ Technol 2021 Apr 7:1-18. Epub 2021 Apr 7.

Department of Chemical and Biochemical Engineering, University of Western Ontario, London, ON, N6A 5B9, Canada.

Current study addresses a problem of elevated aluminum concentrations deteriorating Khibiny Alkaline Massif groundwater quality. The application of chemometric methods to the field dataset 1999-2018 allows to quantitatively describe the groundwater quality, reveal variability patterns and potential sources of elevated aluminum level in the groundwater. The field dataset contains almost 40% more observations of 12 physicochemical groundwater quality parameters than the dataset analyzed in our previous studies on Khibiny groundwater quality assessment reported in the literature. Read More

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Adaptation and validation of the Screening Questionnaire for Family Abuse of the Elderly in the sociocultural context of Colombia.

Health Soc Care Community 2021 Apr 6. Epub 2021 Apr 6.

Center for Evaluation and Surveys Research, National Institute of Public Health, Cuernavaca, Mexico.

In Colombia, like many countries in the world, due to the increase in population of elderly people, mistreatment has increased, which has physical, psychological and social consequences for the individual and major repercussions on society. The detection of abuse is a complex task, among other aspects, due to the concealment of victims and the lack of valid, reliable detection instruments that are in keeping with the sociocultural context. Professionals responsible for dealing with these situations must have an instrument that allows early detection. Read More

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Combined transoral exoscope and OArm-assisted approach for craniovertebral junction surgery: Light and shadows in single-center experience with improving technologies.

J Craniovertebr Junction Spine 2020 Oct-Dec;11(4):293-299. Epub 2020 Nov 26.

Institute of Neurosurgery, Operative Unit, Research Center and Master II Degree Surgical Approaches Craniovertebral Junction, Fondazione Policlinico Universitario A. Gemelli IRCCS, Catholic University, Rome, Italy.

Background: The introduction of recent innovations in the field of intraoperative imaging and neuronavigation, such as OArm Stealth Station, allows to obtain crucial intraoperative data by performing safer and controlled surgical procedures. As part of the improvement of surgical visual magnification and wide expansion of surgical corridors, the 3D-4K exoscope (EX) represents nowadays an interesting and useful tool. Transoral approach (TOA) represents the historical gold standard direct microsurgical route to ventral craniovertebral junction (CVJ). Read More

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November 2020

A sensory bias overrides learned preferences of bumblebees for honest signals in .

Proc Biol Sci 2021 Apr 7;288(1948):20210161. Epub 2021 Apr 7.

Blandy Experimental Farm, University of Virginia, Boyce, VA, USA.

Insect pollinators readily learn olfactory cues, and this is expected to select for 'honest signals' that provide reliable information about floral rewards. However, plants might alternatively produce signals that exploit pollinators' sensory biases, thereby relaxing selection for signal honesty. We examined the innate and learned preferences of for floral scent phenotypes corresponding to different levels of pollen rewards in the presence and absence of the innately attractive floral volatile compound β-trans-bergamotene. Read More

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Rapid Assessment of Resection Margins During Breast Conserving Surgery Using Intraoperative Flow Cytometry.

Clin Breast Cancer 2021 Mar 8. Epub 2021 Mar 8.

Neurosurgical Institute, University of Ioannina School of Medicine, Ioannina, Greece; Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece. Electronic address:

Background: Positive margins are the most important factor for recurrence of the disease after breast-conserving surgery. Several methods have been developed throughout the years to evaluate the margin status during surgery in an attempt to assist the surgeon in excising the whole tumor at once, a goal that has not yet been accomplished.

Patients And Methods: In our study, we compared intraoperative flow cytometry (iFC) with cytology and pathology in order to evaluate 606 samples of margins and tumors corresponding to 99 patients with invasive ductal carcinoma of no special type and invasive lobular carcinoma obtained from breast-conserving surgeries. Read More

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Self-referencing circular dichroism ion yield measurements for improved statistics using femtosecond laser pulses.

Rev Sci Instrum 2021 Mar;92(3):033001

Universität Kassel, Heinrich-Plett-Str. 40, 34132 Kassel, Germany.

The combination of circular dichroism with laser mass spectrometry via the measurement of ion yields is a powerful tool in chiral recognition, but the measured anisotropies are generally weak. The method presented in this contribution reduces the measurement error significantly. A common path optical setup generates a pair of counter-rotating laser foci in the interaction region of a time-of-flight spectrometer. Read More

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Iterative Development of an Innovative Smartphone-Based Dietary Assessment Tool: Traqq.

J Vis Exp 2021 Mar 19(169). Epub 2021 Mar 19.

Division of Human Nutrition and Health, Wageningen University and Research.

To collect dietary intake data in a fast and reliable manner, a flexible and innovative smartphone application (app) called Traqq was developed (iOS/Android). This app can be used as a food record and 24-h recall (or shorter recall periods). Different sampling schemes can be created on either prespecified or random days/times within a predetermined period for both methods, with push notifications to urge the participants to register their food intake. Read More

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Adaptive divergence for rapid adversarial optimization.

PeerJ Comput Sci 2020 18;6:e274. Epub 2020 May 18.

Laboratory of Methods for Big Data Analysis, National Research University Higher School of Economics, Moscow, Russia.

Adversarial Optimization provides a reliable, practical way to match two implicitly defined distributions, one of which is typically represented by a sample of real data, and the other is represented by a parameterized generator. Matching of the distributions is achieved by minimizing a divergence between these distribution, and estimation of the divergence involves a secondary optimization task, which, typically, requires training a model to discriminate between these distributions. The choice of the model has its trade-off: high-capacity models provide good estimations of the divergence, but, generally, require large sample sizes to be properly trained. Read More

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A application for automated H-Reflex measurements and analyses.

Biomed Signal Process Control 2021 Apr 22;66. Epub 2021 Feb 22.

Temple University, College of Engineering, Philadelphia, PA, USA.

Objective: H-Reflex is a test that is carried out to measure the relative excitability of reflex pathways. Although reliable, conventional methods consist of performing many small steps, which requires a high level of attentiveness, and thus can carry an elevated risk of human error, despite proper training. Equipment that is available to perform those tests with different levels of automation are typically proprietary, inextensible by the user, and expensive. Read More

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Novel strategies for the characterization of cancellous bone morphology: Virtual isolation and analysis.

Am J Phys Anthropol 2021 Apr 3. Epub 2021 Apr 3.

Department of Biology, Università di Pisa, Pisa, Italy.

Objectives: The advent of micro-computed tomography (μCT) made cancellous bone more accessible than ever before. Nevertheless, the characterization of cancellous bone is made difficult by its inherent complexity and the difficulties in defining homology across datasets. Here we propose novel virtual methodological approaches to overcome those issues and complement existing methods. Read More

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Prospective Validation of Indocyanine Green Lymphangiography Staging of Breast Cancer-Related Lymphedema.

Cancers (Basel) 2021 Mar 26;13(7). Epub 2021 Mar 26.

Department of Plastic Surgery, Research Unit for Plastic Surgery, Odense University Hospital, 5000 Odense, Denmark.

Indocyanine green lymphangiography (ICG-L) allows real-time investigation of lymphatics. Plastic surgeons performing lymphatic reconstruction use the ICG-L for patient selection and stratification using the MD Anderson (MDA) and the Arm Dermal Backflow (ADB) grading systems. However, the applicability of ICG-L in evaluating breast cancer-related lymphedema (BCRL) is sparse and not well established. Read More

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Advanced Imaging Analysis in Prostate MRI: Building a Radiomic Signature to Predict Tumor Aggressiveness.

Diagnostics (Basel) 2021 Mar 26;11(4). Epub 2021 Mar 26.

Department of Radiology, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.

Radiomics allows the extraction quantitative features from imaging, as imaging biomarkers of disease. The objective of this exploratory study is to implement a reproducible radiomic-pipeline for the extraction of a magnetic resonance imaging (MRI) signature for prostate cancer (PCa) aggressiveness. One hundred and two consecutive patients performing preoperative prostate multiparametric magnetic resonance imaging (mpMRI) and radical prostatectomy were enrolled. Read More

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