BMC Cell Biol 2017 05 25;18(1):23. Epub 2017 May 25.
Laboratory of Cellular and Molecular Biology, Center for Cancer Research, 37 Convent Drive, Bldg.37/Rm2066, NCI, NIH, Bethesda, MD, 20892-4256, USA.
Background: In Dictyostelium discoideum, vesicular transport of the adenylyl cyclase A (ACA) to the posterior of polarized cells is essential to relay exogenous 3',5'-cyclic adenosine monophosphate (cAMP) signals during chemotaxis and for the collective migration of cells in head-to-tail arrangements called streams.
Results: Using fluorescence in situ hybridization (FISH), we discovered that the ACA mRNA is asymmetrically distributed at the posterior of polarized cells. Using both standard estimators and Monte Carlo simulation methods, we found that the ACA mRNA enrichment depends on the position of the cell within a stream, with the posterior localization of ACA mRNA being strongest for cells at the end of a stream. Read More