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BALM: Watching the Formation of Tethered Bilayer Lipid Membranes with Submicron Lateral Resolution.

Langmuir 2021 Jul 29. Epub 2021 Jul 29.

Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, ICBMS, UMR 5246, GEMBAS Team, Lederer building, 1 rue Victor Grignard, F-69622 Villeurbanne, France.

Tethered bilayer lipid membranes (tBLMs) are artificial membranes largely used for the study of biological membranes and membrane-associated proteins. To date, the formation of these membranes was essentially monitored by surface averaging techniques like surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D), which cannot provide both local and real-time information in a single approach. Here, we report an original application of backside absorbing layer microscopy (BALM), a novel white-light wide-field optical microscopy, to study tBLMs. Read More

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Differential Use of Pediatric Video Visits by a Diverse Population During the COVID-19 Pandemic: A Mixed-Methods Study.

Front Pediatr 2021 12;9:645236. Epub 2021 Jul 12.

Department of Pediatrics, University of California, Davis, Sacramento, CA, United States.

To describe and explore pediatric ambulatory video visit use by patient characteristics during the coronavirus disease 2019 (COVID-19) pandemic. We conducted an explanatory sequential mixed methods study with integration at the design and methods level. Phase 1 was a cross-sectional analysis of general and specialty pediatric ambulatory encounters to profile the use of video visits by patient characteristics. Read More

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"From doing to knowing": medical students' experiences of working as Healthcare Assistants.

J Interprof Care 2021 Jul 28:1-7. Epub 2021 Jul 28.

Centre for Interprofessional Practice, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, UK.

There is growing recognition that doctors need to deliver person-centered care. More evidence is needed on how to best equip students in an already busy curriculum. Providing medical students with the opportunity to work as Healthcare Assistants (HCAs) can help them develop the desired skills. Read More

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Validation and implementation of the Panbio COVID-19 Ag rapid test for the diagnosis of SARS-CoV-2 infection in symptomatic hospital healthcare workers.

Infect Prev Pract 2021 Jun 16;3(2):100142. Epub 2021 Apr 16.

Department of Medical Microbiology, Jeroen Bosch Hospital, 's Hertogenbosch, the Netherlands.

Background: RT-PCR is the current recommended laboratory method to diagnose SARS-CoV-2 in healthcare workers (HCW). As RT-PCR is not widely available and is time-consuming, it limits decision making on removal from and return to work of possibly contagious HCW.

Aim: In this study we evaluated the Panbio™ COVID-19 Ag rapid test (PanbioCAgRT) in 825 hospital HCW. Read More

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Double Proton Tautomerism via Intra- or Intermolecular Pathways? The Case of Tetramethyl Reductic Acid Studied by Dynamic NMR: Hydrogen Bond Association, Solvent and Kinetic H/D Isotope Effects.

Molecules 2021 Jul 20;26(14). Epub 2021 Jul 20.

Department of Physics, St. Petersburg State University, 198504 St. Petersburg, Russia.

Using dynamic liquid-state NMR spectroscopy a degenerate double proton tautomerism was detected in tetramethyl reductic acid (TMRA) dissolved in toluene- and in CDCl. Similar to vitamin C, TMRA belongs to the class of reductones of biologically important compounds. The tautomerism involves an intramolecular HH transfer that interconverts the peripheric and the central positions of the two OH groups. Read More

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A comprehensive study on the phenomenon of total breakthrough in liquid chromatography.

J Chromatogr A 2021 Jul 9;1653:462399. Epub 2021 Jul 9.

Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, 5 rue de la Doua, Villeurbanne 69100, France. Electronic address:

Differences in elution strength between the sample solvent and the mobile phase usually give rise to undesirable effects on the chromatographic separation, which may range from slight broadening to severe peak deformation or even splitting. In the most extreme case, the retention factor of the analyte at the head of the column is so small at the time of injection that part of the analyte goes through the column with very little interaction with the stationary phase and hence elutes very close to the column dead time. This phenomenon is known as breakthrough. Read More

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Sildenafil Citrate and Risk of Biochemical Recurrence in Prostate Cancer Patients Treated With Radiation Therapy: Post-Hoc Analysis of a Randomized Controlled Trial.

J Sex Med 2021 Jul 15. Epub 2021 Jul 15.

Memorial Sloan Kettering Cancer Center, Department of Radiation Oncology, New York, NY, USA. Electronic address:

Background: Sildenafil citrate has been shown to be protective of sexual function when given concurrently and following prostate radiation therapy (RT), but some evidence suggests an increased biochemical recurrence (BCR) risk in patients taking sildenafil after radical prostatectomy.

Aim: To evaluate whether sildenafil use is associated with increased risk of BCR in patients receiving prostate RT, we performed a secondary analysis of a randomized placebo-controlled trial (RPCT) that compared sildenafil citrate to placebo during and after prostate RT.

Methods: The study population consisted of prostate cancer patients who initiated radiation treatment at our institution and participated in our multi-institutional RPCT that compared 6 months of sildenafil 50 mg once a day to placebo with a 24-month follow-up. Read More

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Forebrain neural precursor cells are differentially vulnerable to Zika virus infection.

eNeuro 2021 Jul 16. Epub 2021 Jul 16.

Department of Anatomy and Neurobiology, Boston University School of Medicine, Boston, MA 02118

Prenatal exposure to Zika virus (ZIKV) can result in microencephaly and congenital Zika syndrome, though some brain cells and structures are spared by the virus for unknown reasons. Here, a novel murine model of fetal ZIKV infection incorporating intraventricular infection and cell type specific electroporation was used to identify the time course of ZIKV infection and to determine the identity of cells that are initially infected or spared during neocortical neurogenesis. time course studies revealed the presence of ZIKV in apical radial glial cells (aRGCs) at early time points following virus exposure, while basal intermediate progenitor cells (bIPCs) became maximally (ZIKV) after 3 days of virus exposure. Read More

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Posterior hypothalamus as a target in the treatment of aggression: From lesioning to deep brain stimulation.

Handb Clin Neurol 2021 ;182:95-106

Neurosurgery Unit, Department of Neuroscience, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

Intermittent explosive disorder can be described as a severe "affective aggression" condition, for which drugs and other supportive therapies are not fully effective. In the first half of the 19th century, experimental studies progressively increased knowledge of aggressive disorders. A neurobiologic approach revealed the posterior hypothalamic region as a key structure for the modulation of aggression. Read More

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Sleep disorders and the hypothalamus.

Handb Clin Neurol 2021 ;182:369-385

Department of Neurology, James Cook University Hospital, Middlesbrough, United Kingdom.

As early as the 1920s, pathological studies of encephalitis lethargica allowed Von Economo to correctly identify hypothalamic damage as crucial for the profound associated sleep-related symptoms that helped define the condition. Only over the last 3 decades, however, has the key role of the hypothalamus in sleep-wake regulation become increasingly recognized. As a consequence, a close relation between abnormal sleep symptomatology and hypothalamic pathology is now widely accepted for a variety of medical disorders. Read More

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From Clinical Clues to Final Diagnosis: The Return of Detective Work to Clinical Medicine in Cardiac Amyloidosis.

Front Cardiovasc Med 2021 25;8:644508. Epub 2021 Jun 25.

Emergency Department, Amyloid Research and Treatment Center, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Policlinico San Matteo Foundation, Internal Medicine Department, University of Pavia, Pavia, Italy.

Cardiac amyloidosis is frequently misdiagnosed, denying patients the opportunity for timely and appropriate management of the disease. The purpose of this review and case studies is to raise awareness of the diagnostic "red flags" associated with cardiac amyloidosis and the currently available non-invasive strategies for diagnosis. The review focuses on the identification of one of the two main types of cardiac amyloidosis, transthyretin amyloid cardiomyopathy, and non-invasive tools to distinguish this from light-chain amyloidosis. Read More

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Beta-Blocker Therapy Is Associated With Increased 1-Year Survival After Hip Fracture Surgery: A Retrospective Cohort Study.

Anesth Analg 2021 Jul 14. Epub 2021 Jul 14.

School of Medical Sciences, Orebro University, Orebro, Sweden.

Background: The high mortality rates seen within the first postoperative year after hip fracture surgery have remained relatively unchanged in many countries for the past 15 years. Recent investigations have shown an association between beta-blocker (BB) therapy and a reduction in risk-adjusted mortality within the first 90 days after hip fracture surgery. We hypothesized that preoperative, and continuous postoperative, BB therapy may also be associated with a decrease in mortality within the first year after hip fracture surgery. Read More

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"Nerve-sparing" laparoscopic treatment of parametrial ectopic pregnancy.

Fertil Steril 2021 Jul 9. Epub 2021 Jul 9.

Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, Italy; Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy.

Objective: To demonstrate laparoscopic surgical management of parametrial ectopic pregnancy.

Design: Video presentation of laparoscopic nerve-sparing treatment of parametrial pregnancy.

Setting: Tertiary university center. Read More

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Clinical relevance of SARS-CoV-2 infection in late pregnancy.

BMC Pregnancy Childbirth 2021 Jul 12;21(1):505. Epub 2021 Jul 12.

Department of Woman, New-Born and Child, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Mangiagalli Centre, Via M. Fanti, 6, 20122, Milan, Italy.

Background: Evidence on the outcome of SARS-CoV-2 infection in pregnancy is generally reassuring but yet not definitive.

Methods: To specifically assess the impact of SARS-CoV-2 infection in late pregnancy, we prospectively recruited 315 consecutive women delivering in a referral hospital located in Lombardy, Italy in the early phase of the epidemic. Restriction of the recruitment to this peculiar historical time period allowed to exclude infections occurring early in pregnancy and to limit the recall bias. Read More

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Reining in BTK: Interdomain Interactions and Their Importance in the Regulatory Control of BTK.

Front Cell Dev Biol 2021 23;9:655489. Epub 2021 Jun 23.

Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, United States.

Since Dr. Ogden Bruton's 1952 paper describing the first human primary immunodeficiency disease, the peripheral membrane binding signaling protein, aptly named Bruton's tyrosine kinase (BTK), has been the target of intense study. Dr. Read More

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Utilization of a New Customizable Scoring Tool to Recruit and Select Pulmonary/Critical Care Fellows.

Cureus 2021 Jun 2;13(6):e15396. Epub 2021 Jun 2.

Educational Evaluation and Policy Studies, Virginia Tech Carilion School of Medicine, Roanoke, USA.

Background: Finding the ideal candidate for a residency/fellowship program has always been difficult. Finding the "perfect" match has always been the ultimate goal. However, many factors affect obtaining that "perfect" match. Read More

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Resequencing and transcriptomic analysis reveal differences in nitrite reductase in jujube fruit (Ziziphus jujuba Mill.).

Plant Methods 2021 Jul 12;17(1):75. Epub 2021 Jul 12.

College of Horticulture, Shanxi Agricultural University, Taigu, 030801, China.

Background: Jujube is a typical fruit tree species from China. 'Muzao', a cracking-susceptible cultivar, and 'Linhuang No. 1', a cracking-resistant cultivar, were selected in a previous study as contrasting research materials. Read More

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Percolation-intercropping strategies to prevent dissemination of phytopathogens on plantations.

Chaos 2021 Jun;31(6):063105

Centro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio VAL 1 - Ecocampus Valsequillo, Km 1.7 carretera a San Baltazar Tetela, San Pedro Zacachimalpa, 72960, Puebla, Puebla, Mexico.

Phytophthora is one of the most aggressive and worldwide extended phytopathogens that attack plants and trees. Its effects produce tremendous economical losses in agronomy and forestry since no effective fungicide exists. We propose to combine percolation theory with an intercropping sowing configuration as a non-chemical strategy to minimize the dissemination of the pathogen. Read More

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A Case of Tumor-Induced Osteomalacia: Finding the Culprit Acetabular Tumor and Successful Resection with a Novel Hip Joint-Preserving Surgery.

J Orthop Case Rep 2021 Mar;11(3):37-41

Department of Medicine, Division of Endocrinology, Diabetes and Metabolism, The University of Pennsylvania, Philadelphia, PA, United States.

Introduction: Tumor-induced osteomalacia (TIO) is a rare paraneoplastic disorder caused by tumors that produce fibroblast growth factor 23 (FGF23) resulting in phosphate wasting and inadequate bone mineralization. Complete resection of the tumor can be curative. However, these tumors are typically difficult to find anatomically due to small size and location. Read More

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Enzymatic oxidation of oleuropein and 3-hydroxytyrosol by laccase, peroxidase, and tyrosinase.

J Food Biochem 2021 Jul 4:e13803. Epub 2021 Jul 4.

Microbial Enzymology Group (MEG), Department of Applied Sciences, Northumbria University, Newcastle Upon Tyne, UK.

The oxidation of oleuropein and 3-hydroxytyrosol by oxidases laccase, tyrosinase, and peroxidase has been studied. The use of a spectrophotometric method and another spectrophotometric chronometric method has made it possible to determine the kinetic parameters V and K for each enzyme. The highest binding affinity was shown by laccase. Read More

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Smartphone use and addiction during the coronavirus disease 2019 (COVID-19) pandemic: cohort study on 184 Italian children and adolescents.

Ital J Pediatr 2021 Jul 2;47(1):150. Epub 2021 Jul 2.

Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties "G. D'Alessandro", University of Palermo, Palermo, Italy.

Background: The lives of many children and adolescents are today increasingly influenced by new technological devices, including smartphones. The coronavirus disease 2019 (COVID-19) pandemic occurred in a time of outstanding scientific progress and global digitalization. Young people had relevant adverse psychological and behavioral effects due to the COVID-19 pandemic, mainly related to infection control measures, which led them to spend more time at home and with major use of technological tools. Read More

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Nurses and midwives' experiences with peer-group clinical supervision intervention: A pilot study.

J Nurs Manag 2021 Jul 2. Epub 2021 Jul 2.

Catherine McAuley School of Nursing and Midwifery, University College Cork, Cork, Ireland.

Aim: This study aimed to evaluate differences in supervisees' understanding of clinical supervision and their perceptions of organisational functioning before and after engaging in peer-group clinical supervision.

Background: Protected reflective time allows discussion of complex issues affecting health care. Peer-group clinical supervision is one model of clinical supervision that could facilitate this, but it is poorly understood. Read More

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Reality Check 2: The Cost-Effectiveness of Policy Disallowing Body Checking in Non-Elite 13- to 14-Year-Old Ice Hockey Players.

Int J Environ Res Public Health 2021 06 11;18(12). Epub 2021 Jun 11.

Department of Paediatrics, Cumming School of Medicine, University of Calgary, Calgary, AB T3B 6A8, Canada.

Sport-related injuries are the leading cause of injury in youth and are costly to the healthcare system. When body checking is disallowed in non-elite levels of Bantam (ages 13-14 years) ice hockey, the injury rate is reduced, but the impact on costs is unknown. This study compared rates of game injuries and costs among non-elite Bantam ice hockey leagues that disallow body checking to those that did not. Read More

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Addressing the Data Acquisition Paradigm in the Early Detection of Pediatric Foot Deformities.

Sensors (Basel) 2021 Jun 28;21(13). Epub 2021 Jun 28.

Intelligence for Embedded Systems-Research Line, SDAS Research Group, Ibarra 100150, Ecuador.

The analysis of plantar pressure through podometry has allowed analyzing and detecting different types of disorders and treatments in child patients. Early detection of an inadequate distribution of the patient's weight can prevent serious injuries to the knees and lower spine. In this paper, an embedded system capable of detecting the presence of normal, flat, or arched footprints using resistive pressure sensors was proposed. Read More

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New Therapies to Correct the Cystic Fibrosis Basic Defect.

Int J Mol Sci 2021 Jun 8;22(12). Epub 2021 Jun 8.

Department of Medicine, Respiratory Division, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC J1H 5N4, Canada.

Rare diseases affect 400 million individuals worldwide and cause significant morbidity and mortality. Finding solutions for rare diseases can be very challenging for physicians and researchers. Cystic fibrosis (CF), a genetic, autosomal recessive, multisystemic, life-limiting disease does not escape this sad reality. Read More

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In silico re-foundation of strain-based healing assessment of fractures treated with an external fixator.

J Mech Behav Biomed Mater 2021 09 3;121:104619. Epub 2021 Jun 3.

Department of Civil and Industrial Engineering, Università di Pisa, Largo Lucio Lazzarino 2, 56122, Pisa, Italy. Electronic address:

In the last decades, the literature has demonstrated a renewed interest in finding quantitative and non-invasive techniques for the assessment of bone fractures, by replacing X-ray images. Many different approaches have been proposed from ultrasounds to vibrations. This study aims to numerically assess the foundation of a method firstly proposed in 70' years, based on strain gauges measurements on external fixators for fracture healing monitoring. Read More

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September 2021

Why Train Together When Racing Is Performed Alone? Drafting in Long-Track Speed Skating.

Int J Sports Physiol Perform 2021 Jun 30:1-6. Epub 2021 Jun 30.

Purpose: In long-track speed skating, drafting is a commonly used phenomenon in training; however, it is not allowed in time-trial races. In speed skating, limited research is available on the physical and psychological impact of drafting. The aim of this study was to determine the influence of "skating alone," "leading," or "drafting" on physical intensity (heart rate and blood lactate) and perceived intensity (perceived exertion) of speed skaters. Read More

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Evolution of plant telomerase RNAs: farther to the past, deeper to the roots.

Nucleic Acids Res 2021 07;49(13):7680-7694

Department of Cell Biology and Radiobiology, Institute of Biophysics of the Czech Academy of Sciences, Brno CZ-61265, Czech Republic.

The enormous sequence heterogeneity of telomerase RNA (TR) subunits has thus far complicated their characterization in a wider phylogenetic range. Our recent finding that land plant TRs are, similarly to known ciliate TRs, transcribed by RNA polymerase III and under the control of the type-3 promoter, allowed us to design a novel strategy to characterize TRs in early diverging Viridiplantae taxa, as well as in ciliates and other Diaphoretickes lineages. Starting with the characterization of the upstream sequence element of the type 3 promoter that is conserved in a number of small nuclear RNAs, and the expected minimum TR template region as search features, we identified candidate TRs in selected Diaphoretickes genomes. Read More

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Compensatory epistasis explored by molecular dynamics simulations.

Hum Genet 2021 Jun 26. Epub 2021 Jun 26.

i3S, Instituto de Investigação e Inovação em Saúde, Population Genetics and Evolution Group, Universidade do Porto, Rua Alfredo Allen 208, 4200-135, Porto, Portugal.

A non-negligible proportion of human pathogenic variants are known to be present as wild type in at least some non-human mammalian species. The standard explanation for this finding is that molecular mechanisms of compensatory epistasis can alleviate the mutations' otherwise pathogenic effects. Examples of compensated variants have been described in the literature but the interacting residue(s) postulated to play a compensatory role have rarely been ascertained. Read More

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Assisted Reproductive Techniques, ADHD, and School Performance.

Pediatrics 2021 Jun 25. Epub 2021 Jun 25.

Departments of Medical Epidemiology and Biostatistics and

Objectives: To examine the long-term impact of assisted reproductive techniques (ART) on offspring neurodevelopment, accounting for parental factors and the role of infertility.

Methods: Linkage of national registers allowed follow-up of >2.4 million children born in Sweden 1986-2012. Read More

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