394 results match your criteria alkaline hydrolysates

Optimization of protein hydrolysis conditions and antioxidant activity of tetrapeptide Asp-Arg-Glu-Leu by elevating the Nrf2/Keap1-p38/PI3K-MafK signaling pathway.

Food Funct 2021 Jun;12(11):4808-4824

Beijing Laboratory of Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China.

Tetrapeptide Asp-Arg-Glu-Leu (DREL) was isolated from Jiuzao protein hydrolysates (JPHs) by alkaline proteinase (AP) and exhibited antioxidant activity in the HepG2 cell model in the previous study. In this study, the hydrolysis method of Jiuzao protein (JP) was further optimized by using different proteinases under different conditions (i.e. Read More

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Effect of different structural flaxseed lignans on the stability of flaxseed oil-in-water emulsion: An interfacial perspective.

Food Chem 2021 Apr 8;357:129522. Epub 2021 Apr 8.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, and Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Wuhan 430062, China. Electronic address:

The influences of the different structural flaxseed lignans on flaxseed oil (FO) emulsions during storage and digestion were investigated, focusing on their interfacial behavior. From perspective of interface, more than 60% of secoisolariciresinol (SECO) and the acidic hydrolysates of flaxseed lignan macromolecule (FLEH) were located on the interface of FO emulsions. It improved the stability of FO emulsions both during storage and digestion by inhibiting of free radical penetration and improving their targeted antioxidative activity. Read More

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Industrial sustainability of microbial keratinases: production and potential applications.

World J Microbiol Biotechnol 2021 Apr 17;37(5):86. Epub 2021 Apr 17.

Instituto de Biociências, Letras e Ciências Exatas - Universidade Estadual Paulista "Júlio de Mesquita Filho", R. Cristóvão Colombo, 2265. Jd Nazareth, Ibilce-Unesp, São José do Rio Preto, São Paulo, Brazil.

Keratinases are proteolytic enzymes with a particular ability to cleave peptide bonds in keratin, and in other proteins. Due to their broad-spectrum of activity, keratinases are considered viable substitutes for chemical and thermal treatments of protein-rich industrial by-products. Among these protein residues, special attention has been given to keratinous materials (feathers, hair, horns, etc. Read More

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Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts.

Foods 2021 Feb 3;10(2). Epub 2021 Feb 3.

Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, Chile.

Defatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with a high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study is to obtain an enzymatic hydrolysate of rapeseed protein that inhibits proliferation on a breast cancer cell line (MCF-7), but not healthy human fibroblast cells. The DRM was solubilized in an alkaline medium to obtain an alkaline rapeseed extract (RAE). Read More

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February 2021

Polyhydroxybutyrate production from ultrasound-aided alkaline pretreated finger millet straw using Bacillus megaterium strain CAM12.

Bioresour Technol 2021 Apr 8;325:124632. Epub 2021 Jan 8.

Centro de Investigación en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile; Scientific and Technological Bioresource Nucleus, BIOREN-UFRO, Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile. Electronic address:

In this study, finger millet straw (FMS) was utilized for the production of Polyhydroxybutyrate (PHB) by Bacillus megaterium strain CAM12. Ultrasound-assisted alkaline (NaOH) pretreatment of FMS under optimized conditions followed by enzymatic saccharification resulted in the maximum delignification (72%), hydrolysis yield (84%), glucose yield (86%) and xylose yield (61%). The effects of different pH, temperature, incubation period, inoculum concentration, agitation speed and FMS enzymatic hydrolysates concentration were investigated to improve the PHB production. Read More

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Digestive tract morphology and enzyme activities of juvenile diploid and triploid Atlantic salmon (Salmo salar) fed fishmeal-based diets with or without fish protein hydrolysates.

PLoS One 2021 11;16(1):e0245216. Epub 2021 Jan 11.

Faculty of Biosciences, Fisheries and Economics, UiT - the Arctic University of Norway, Tromsø, Norway.

Triploid, sterile Atlantic salmon (Salmo salar) could make a contribution to the development of the farming industry, but uncertainties about the performance and welfare of triploids have limited their adoption by farmers. In this study, we compared the ontogeny of digestive tract morphology and enzyme activities (pepsin, trypsin, chymotrypsin, alkaline phosphatase and aminopeptidase) of diploid and triploid Atlantic salmon. Fish were fed diets based on fishmeal (STD) or a mix of fishmeal and hydrolysed fish proteins (HFM) whilst being reared at low temperature from start-feeding to completion of the parr-smolt transformation. Read More

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Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method.

Molecules 2020 Nov 22;25(22). Epub 2020 Nov 22.

Department of Biogeochemistry and Soil Science, University of Science and Technology, 6 Bernardynska St., 85-029 Bydgoszcz, Poland.

The aim of this paper was to determine the effect of the hydrolysis method on the amounts of phenolic compounds in the plant material in soil and, as a consequence, on the parameters to determine the degree of lignins transformation in soils. The study included the plant material (hay, sward, and roots) and soil-Albic Brunic Arenosol (horizon A, AE, and Bsv) samples. Phenolic compounds were isolated at two stages by applying acid hydrolysis followed by alkaline re-hydrolysis. Read More

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November 2020

Baijiu vinasse as a new source of bioactive peptides with antioxidant and anti-inflammatory activity.

Food Chem 2021 Mar 25;339:128159. Epub 2020 Sep 25.

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address:

During production in Chinese baijiu fermentation process, huge amounts of the by-product vinasse are generated and generally utilized as low-value animal feed. We applied alkaline extraction in combination with ultrasonication to recover vinasse proteins, which were then hydrolyzed by complex protease Corolase PP for 8 h to obtain peptide fractions (VPH-1, -2, -3) displaying high DPPH radical scavenging activity. VPH-3 (<3 kDa) separated by ultrafiltration had EC values lower than those of VPH-1 and -2 for reactive oxygen species (ROS) and reactive nitrogen species (RNS) radicals, and significantly inhibited production of NO and pro-inflammatory cytokines in LPS-stimulated RAW264. Read More

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3D PCL/fish collagen composite scaffolds incorporating osteogenic abalone protein hydrolysates for bone regeneration application: and studies.

J Biomater Sci Polym Ed 2021 Feb 22;32(3):355-371. Epub 2020 Oct 22.

Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan, Republic of Korea.

Three-dimensional (3 D) printing is an effective technology that has shown considerable potential for use in tissue regeneration. Of the many materials that have been proposed for this purpose, poly (ε-caprolactone) (PCL) 3 D scaffolds have been received significant attention in the bone tissue engineering field due to its advantageous mechanical properties and biocompatibility. In this study, a novel method was developed for tissue-engineered bone that combines PCL 3 D scaffolds with fish collagen (Col) and the osteogenic abalone intestine gastro-intestinal digests (AIGIDs) from . Read More

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February 2021

Stability and cytotoxicity of DPPH inhibitory peptides derived from biodegradation of chicken feather.

Protein Expr Purif 2021 01 7;177:105748. Epub 2020 Sep 7.

Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Tehran, Iran. Electronic address:

The antioxidant activity and cell viability of feather hydrolysates obtained with the Bacillus licheniformis were evaluated using an in-vitro model. The results indicate that feathers-derived peptides under 3 kDa have antioxidant activity with IC values of 5.03 ± 0. Read More

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January 2021

Physicochemical properties of soy protein hydrolysate and its formulation and stability with encapsulated probiotic under in vitro gastrointestinal environment.

J Food Sci 2020 Oct 31;85(10):3543-3551. Epub 2020 Aug 31.

Institute for Nanoscience and Engineering, University of Arkansas, 731 W. Dickson St., Fayetteville, AR, 72704, U.S.A.

The objective of this study was to prepare protein isolate from defatted soybean and identify an optimal hydrolysis protocol to create improved hydrolysates and ascertain the optimum encapsulation technique for probiotics. Soy protein isolate (SPI) was prepared using an alkaline extraction procedure for solubility within a neutral, beverage-specific pH range. The soy protein hydrolysate (SPH) was prepared from aqueous extracted SPI using pepsin. Read More

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October 2020

Bioactive peptides derived from plant origin by-products: Biological activities and techno-functional utilizations in food developments - A review.

Food Res Int 2020 10 2;136:109504. Epub 2020 Jul 2.

Aydın Adnan Menderes University, Engineering Faculty, Food Engineering Department, 09010 Efeler, Aydın, Turkey. Electronic address:

Agro-industrial by-products containing considerable amounts of protein (10-50%) such as soybean meal, rice bran and coconut pulp are promising bioactive peptide sources with annual disposal rate of 800 million tons in the world. More recently, plant by-products rich in protein content have been studied under various prisms that include recovery techniques, peptide production methods, determination of technological benefits and functional properties, and their applications in foods. The researches in bioactive peptides provide evidence over the techno-functional properties and the health benefits are highly dependent upon their amino acid sequences, molecular weights, conformations and surface properties. Read More

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October 2020

Poly(3-hydroxybutyrate-3-hydroxyvalerate) production from pretreated waste lignocellulosic hydrolysates and acetate co-substrate.

Bioresour Technol 2020 Nov 28;316:123911. Epub 2020 Jul 28.

College of Light Industry Science and Engineering, Tianjin University of Science & Technology, Tianjin 300222, PR China.

The purpose of this study was to explore the potential of producing Poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) by mixed microbial culture (MMC) with lignocellulosic hydrolysates and acetate co-substrate as feedstock. The addition of co-substrate acetate led to the introduction of HV monomer into the polyhydroxyalkanoate (PHA), and the initial mixed sludge suspension (MLSS) increased with the increase of acetate. Almost 1. Read More

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November 2020

Utilization of Noxious Weed Water Hyacinth Biomass as a Potential Feedstock for Biopolymers Production: A Novel Approach.

Polymers (Basel) 2020 Jul 29;12(8). Epub 2020 Jul 29.

Department of Environmental Science and Engineering, Ewha Womans University, Seoul 120-750, Korea.

This study aims to utilize a noxious weed water hyacinth biomass (WH) for polyhydroxybutyrate (PHB) production. Alkaline and peracetic acid pretreatment was employed for the hydrolysis of WH and consequently enzymatic saccharification to produce fermentable sugars for PHB production. The pretreatment competence was determined using various operational parameters. Read More

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Oxidative destruction of phenol on Fe/SiO catalysts.

Water Sci Technol 2020 May;81(10):2189-2201

Far Eastern Federal University, Vladivostok, Russian Federation E-mail:

In the present study, the sol-gel technique helped to obtain Fe-containing samples with a base of amorphous silicon dioxide from rice husks RH-Fe and RH-Fe-300. These materials are characterized by IR spectroscopy, X-ray phase and X-ray spectral analysis. It was shown that the samples contain silicate structures Si-O-Si(Fe) and are in an amorphous state. Read More

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A microalgal-based preparation with synergistic cellulolytic and detoxifying action towards chemical-treated lignocellulose.

Plant Biotechnol J 2021 01 2;19(1):124-137. Epub 2020 Sep 2.

Dipartimento di Biotecnologie, Università di Verona, Verona, Italy.

High-temperature bioconversion of lignocellulose into fermentable sugars has drawn attention for efficient production of renewable chemicals and biofuels, because competing microbial activities are inhibited at elevated temperatures and thermostable cell wall degrading enzymes are superior to mesophilic enzymes. Here, we report on the development of a platform to produce four different thermostable cell wall degrading enzymes in the chloroplast of Chlamydomonas reinhardtii. The enzyme blend was composed of the cellobiohydrolase CBM3GH5 from C. Read More

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January 2021

Keratinases from Coriolopsis byrsina as an alternative for feather degradation: applications for cloth cleaning based on commercial detergent compatibility and for the production of collagen hydrolysate.

Biotechnol Lett 2020 Nov 8;42(11):2403-2412. Epub 2020 Jul 8.

Instituto de Biociências, Letras e Ciências Exatas-Universidade Estadual Paulista Júlio de Mesquita Filho -São José do Rio Preto, São Paulo, Brazil.

Objectives: Keratinases are proteolytic enzymes that emerge as an alternative for dealing with the disposal of chicken feathers. In this study, we aimed to investigate the keratin-degrading enzymes secreted by the fungus Coriolopsis byrsina and their partial biochemical characterization to adapt their use for keratin decomposition, detergent additive applications, and collagen degradation.

Results: We observed the secretion of different proteolytic enzymes that possessed caseinolytic activity that peaked at pH 7. Read More

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November 2020

Cell Proliferation Stimulation Ability and Osteogenic Activity of Low Molecular Weight Peptides Derived from Bovine Gelatin Hydrolysates.

J Agric Food Chem 2020 Jul 7;68(29):7630-7640. Epub 2020 Jul 7.

Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

It has been recognized that collagen peptides of MW 878 Da (CP1) promote osteoblast proliferation and mineralization. The objective of this study is to identify the peptides responsible for proliferation of osteoblast growth, enhancement of ALP (alkaline phosphatase) activity in osteoblasts and promotion of osteoblast mineralization. To this end, the CP1 were fractioned by a series of chromatography procedures, and 51 peptides from the fraction possessing the most powerful cell proliferation ability were identified by LC-MS-MS. Read More

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[Comparative characteristics of enzymatic hydrolysis methods for isolating toxic substances from blood and hair].

Sud Med Ekspert 2020 ;63(3):23-29

St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia.

The aim of study was to develop methods for the hydrolysis with hyaluronidases for the isolation of drugs (phenobarbital, diphenhydramine hydrochloride and phenibut) from biological objects (blood, hair) and to compare their effectivenes with previously developed methods of enzymatic hydrolysis. The studies were carried out using model «blood - model drug substance (MDS)», a natural and artificially colored wool (hair) of laboratory animals - guinea pigs of white, red and black natural colors, which were daily given a solution of MDS and then were subjected to cosmetic effects - coloring. The isolation of MDS from the model complex «blood - MDS» and from wool was carried out using the developed methods of hydrolysis with proteolytic enzymes (papain, chymopsin and chymotrypsin) and hyaluronidase. Read More

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October 2020

Overexpression of SFA1 in engineered Saccharomyces cerevisiae to increase xylose utilization and ethanol production from different lignocellulose hydrolysates.

Bioresour Technol 2020 Oct 21;313:123724. Epub 2020 Jun 21.

College of Life Sciences, Capital Normal University, Beijing 100048, China. Electronic address:

Here, an engineered Saccharomyces cerevisiae strain SFA1 was constructed by overexpressing SFA1 in a reported WXY70 with effective six-gene clusters. Under simulated maize hydrolysate, SFA1 produced an ethanol yield of 0.492 g/g totalsugars within 48 h. Read More

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October 2020

Effects of the Whey Protein Hydrolysates of Various Protein Enzymes on the Proliferation and Differentiation of 3T3-E1 Osteoblasts.

Prev Nutr Food Sci 2020 Mar;25(1):71-77

Department of Public Health Science, Korea University, Seoul 02841, Korea.

In this study, we used various proteinases to investigate the effect of whey protein hydrolysates on proliferation and differentiation of MC3T3-E1 osteoblasts. To confirm hydrolysis of the whey protein hydrolysates, the yield and α-amino acid content were determined. Since osteogenic cell activity is an important factor in osteogenesis, we evaluated the proliferation of osteogenic cells by measuring 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and alkaline phosphatase (ALP) activity. Read More

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[Comparative study on free and bound phenolic acids before and after drying of Salvia miltiorrhiza].

Zhongguo Zhong Yao Za Zhi 2020 Mar;45(5):1090-1096

Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica,Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China School of Pharmacy, Southwest Medical University Luzhou 646000, China.

There were significant differences in phenolic acid content between fresh and dried Salvia miltiorrhiza before and after drying. That is to say, the content of phenolic acid in S. miltiorrhiza significantly increased with the increase of dehydration during the drying process. Read More

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Mild alkaline pretreatment can achieve high hydrolytic and fermentation efficiencies for rice straw conversion to bioethanol.

Prep Biochem Biotechnol 2020 24;50(8):814-819. Epub 2020 Mar 24.

Biofuels and Biorefineries Section, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram, India.

Mild alkaline pretreatment was evaluated as a strategy for effective lignin removal and hydrolysis of rice straw. The pretreatment efficiency of different NaOH concentrations (0.5, 1. Read More

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A bovine lactoferrin-derived peptide induced osteogenesis via regulation of osteoblast proliferation and differentiation.

J Dairy Sci 2020 May 18;103(5):3950-3960. Epub 2020 Mar 18.

School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150090, China; School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China. Electronic address:

Osteoporosis is a disease of aging, characterized by a decrease in bone quality and a reduction in bone strength. Promoting the activity of osteoblasts is a useful strategy for combating the progression of osteoporosis. As a novel bone growth factor, lactoferrin plays a role in the anabolic activity in bone by inducing the proliferation and differentiation of osteoblasts and inhibiting the formation of osteoclasts. Read More

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Comparison of protein extraction methods from excess activated sludge.

Chemosphere 2020 Jun 3;249:126107. Epub 2020 Feb 3.

School of Water Conservancy Engineering, Zhengzhou University, Zhengzhou, 450001, China.

In this study, we used chemical methods (acid-thermal (AT) and alkaline-thermal (AKT)), enzymatic methods (single enzyme (SE) and composite enzyme (CE)) and assisted enzymatic methods (ultrasonic assisted enzyme (USE) and thermal assisted enzyme (TE)) to extract proteins from excess activated sludge. The advantages and applicability of each method were compared and analyzed in terms of their protein extraction rate (R), energy consumption, material consumption and cost, protein hydrolysates and sludge dewatering performance. The results showed that the R of the chemical methods were more than 75%, which were much higher than those of the enzymatic methods. Read More

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Integrating bran starch hydrolysates with alkaline pretreated soft wheat bran to boost sugar concentration.

Bioresour Technol 2020 Apr 20;302:122826. Epub 2020 Jan 20.

Department of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506, United States. Electronic address:

Soft wheat bran (SWB), one of the most abundant byproducts from the wheat milling industry, is a potential candidate for biofuel production. In this study, bran starch hydrolysates were separately integrated with dilute acid pretreated SWB and alkaline pretreated SWB to boost fermentable sugar concentration. Alkaline pretreatment showed higher sugar recoveries than acid pretreatment. Read More

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Fed-batch enzymatic hydrolysis of alkaline organosolv-pretreated corn stover facilitating high concentrations and yields of fermentable sugars for microbial lipid production.

Biotechnol Biofuels 2020 22;13:13. Epub 2020 Jan 22.

1School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, 947 Heping Road, Wuhan, 430081 People's Republic of China.

Background: Lignocellulosic biomass has been commonly regarded as a potential feedstock for the production of biofuels and biochemicals. High sugar yields and the complete bioconversion of all the lignocellulosic sugars into valuable products are attractive for the utilization of lignocelluloses. It is essential to pretreat and hydrolyze lignocelluloses at high solids loadings during industrial processes, which is more economical and environmentally friendly as capital cost, energy consumption, and water usage can be reduced. Read More

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January 2020

Comparative data on effects of alkaline pretreatments and enzymatic hydrolysis on bioemulsifier production from sugarcane straw by Cutaneotrichosporon mucoides.

Bioresour Technol 2020 Apr 28;301:122706. Epub 2019 Dec 28.

Department of Biotechnology, Engineering School of Lorena, University of São Paulo, Estrada Municipal do Campinho, s/n° 12.602-810, Brazil.

Bioemulsifiers are surface active compounds which could be potentially used in food processing, cosmetic sector and oil recovery. Sugarcane straw (SS), was used as the raw substrate for the production of bio-emulsifiers (BE) by Cutaneotrichosporon mucoides. Three different delignification strategies using dilute sodium hydroxide, sodium sulfite and ammonium hydroxide followed by enzymatic hydrolysis (Cellic CTec 2, 7. Read More

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Ethanol Production and Other Bioproducts by Galactomyces geotrichum from Sugarcane Bagasse Hydrolysate.

Curr Microbiol 2020 May 8;77(5):738-745. Epub 2020 Jan 8.

Federal University of Uberlândia, Agricultural Sciences Institute (ICIAG-UFU), Uberlândia, Minas Gerais, 38405-320, Brazil.

This study investigated the enzymatic saccharification of alkaline-pretreated sugarcane bagasse (PSB) and the bioconversion of simple sugars from hydrolysates to ethanol and other bioproducts by the yeast Galactomyces geotrichum. The effects of percentage of dry substrate (3 and 10% w/v) and time of hydrolysis (24 and 72 h) in the content of released sugars were evaluated. The concentrations of monosaccharides and total reducing sugars (TRS) were calculated by high-performance liquid chromatography (HPLC) and by 3. Read More

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Antioxidant activities of peptide hydrolysates obtained from the seeds of Decne (African breadfruit).

Prep Biochem Biotechnol 2020 3;50(5):504-510. Epub 2020 Jan 3.

Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria.

Protein hydrolysates usually possess higher nutritive value than an equivalent mixture of free amino acids. This study was aimed at determining the antioxidant activities of protein hydrolysates of seeds. Soluble protein was isolated from seed flour by alkaline solubilization and acid precipitation, and further hydrolyzed with three proteases (trypsin, pancreatin and pepsin) individually and in a sequential two- and three-protease systems. Read More

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December 2020