J Biol Chem 2021 Apr 8:100648. Epub 2021 Apr 8.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan; Japan Agency for Medical Research and Development, CREST, Tokyo, Japan. Electronic address:
Natural antibodies, predominantly immunoglobulin M (IgM), play an important role in the defense against pathogens and in maintaining homeostasis against oxidized molecules known as oxidation-specific epitopes, such as those contained in oxidized low-density lipoproteins. However, due to the complexity of the oxidized products, very few individual epitopes have been characterized in detail. In the present study, to identify endogenous sources of oxidation-specific epitopes, we stimulated mouse spleen and peritoneal cavity (PerC) cells in vitro with bovine serum albumin (BSA) modified with a variety of lipid peroxidation-related carbonyl compounds, and identified the acrolein-modified BSA (acrBSA) as the most efficient trigger studied for the production of IgM in PerC cells. Read More