2,033 results match your criteria agent hepg2

Effects of Bacillus subtilis iturin A on HepG2 cells in vitro and vivo.

AMB Express 2021 May 10;11(1):67. Epub 2021 May 10.

Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, 710072, Shaanxi, China.

Iturin A with cyclic peptide and fatty acid chain isolated from Bacillus subtilis fermentation shows a variety of biological activities. Among them, the anticancer activity attracted much attention. However, the molecular mechanism of its inhibitory effect on hepatocellular carcinoma was still unclear. Read More

View Article and Full-Text PDF

Acute Toxicity and DNA Instability Induced by Exposure to Low Doses of Triclosan and Phthalate DEHP, and Their Combinations, .

Front Genet 2021 20;12:649845. Epub 2021 Apr 20.

Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, Santos, Brazil.

Triclosan (TCS) is an antimicrobial agent widely used in personal care products (PCP) and the di-(2-ethyl hydroxy-phthalate) (DEHP) is a chemical compound derived from phthalic acid, used in medical devices and plastic products with polyvinyl chloride (PVCs). As result of their extensive use, TCS and DEHP have been found in the environment and previous studies demonstrated the association between their exposure and toxic effects, mostly in aquatic organisms, but there is a shortage in the literature concerning the exposure of TCS and DEHP in human cells. The aim of the present study was to assess the impact of exposure to TCS and DEHP, as well as their combinations, on biomarkers related to acute toxicity and DNA instability, in HepG2 cells, by use of cytokinesis-block micronucleus cytome (CBMNCyt) assay. Read More

View Article and Full-Text PDF

Putative mechanism for cancer suppression by PLGA nanoparticles loaded with smoke extract.

J Microencapsul 2021 May 5:1-14. Epub 2021 May 5.

Department of Biology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.

Synthesis and investigation of biological activity of smoke-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles. smoke-loaded PLGA nanoparticles (PHSE-PNP) were produced by double emulsion solvent evaporation method and characterised by scanning electron microscopy (SEM), dynamic light scattering (DLS), and -potential. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) for toxicity evaluation, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assay for antioxidant power, chorioallantoic membrane (CAM), qPCR, and scratch assay for angiogenesis and mouse cancer model for antitumor effects of PHSE-PNP's were used. Read More

View Article and Full-Text PDF

RRx-001 Increases Erythrocyte Preferential Adhesion to the Tumor Vasculature.

Int J Mol Sci 2021 Apr 29;22(9). Epub 2021 Apr 29.

Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093-0412, USA.

Red blood cells (RBCs) serve a variety of functions beyond mere oxygen transport both in health and pathology. Notably, RRx-001, a minimally toxic pleiotropic anticancer agent with macrophage activating and vascular normalization properties currently in Phase III trials, induces modification to RBCs which could promote vascular adhesion similar to sickle cells. This study assessed whether RBCs exposed to RRx-001 adhere to the tumor microvasculature and whether this adhesion alters tumor viability. Read More

View Article and Full-Text PDF

Expression and purification of a recombinant ELRL-MAP30 with Dual-targeting anti-tumor bioactivity.

Protein Expr Purif 2021 Apr 29:105893. Epub 2021 Apr 29.

College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. Electronic address:

MAP30 (Momordica antiviral protein 30kD) is a single-chain Ⅰ-type ribosome inactivating protein with a variety of biological activities, including anti-tumor ability. It was reported that MAP30 would serve as a novel and relatively safe agent for prophylaxis and treatment of liver cancer. To determine whether adding two tumor targeting peptides could improve the antitumour activities of MAP30, we genetically modified MAP30 with an RGD motif and a EGFRi motif, which is a ligand with high affinity for αvβ3 integrins and with high affinity for EGFR. Read More

View Article and Full-Text PDF

Effect of on the Liver Protection of Non-Alcoholic Fatty Liver Disease Based on In Vitro and In Vivo Models.

Curr Issues Mol Biol 2021 Apr 28;43(1). Epub 2021 Apr 28.

Department of Food and Nutrition, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, Korea.

Edible insects, (silkworm; SW), which feed on mulberry leaves, have been consumed by humans for a long time as supplements or traditional medication. Non-alcoholic fatty liver disease (NAFLD) is a liver metabolic disorder that affects many people worldwide. We examined the hepatoprotective effects of SW using in vitro and high-fat and high-fructose (HFHF) diet-induced obese in vivo model mice by real-time PCR, immunoblot analysis, and fecal microbiota analysis. Read More

View Article and Full-Text PDF

Cytotoxic Effects and Intracellular Localization of Bin Toxin from in Human Liver Cancer Cell Line.

Toxins (Basel) 2021 04 19;13(4). Epub 2021 Apr 19.

Institute of Molecular Biosciences, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, Thailand.

Binary toxin (Bin toxin), BinA and BinB, produced by has been used as a mosquito-control agent due to its high toxicity against the mosquito larvae. The crystal structures of Bin toxin and non-insecticidal but cytotoxic parasporin-2 toxin share some common structural features with those of the aerolysin-like toxin family, thus suggesting a common mechanism of pore formation of these toxins. Here we explored the possible cytotoxicity of Bin proteins (BinA, BinB and BinA + BinB) against Hs68 and HepG2 cell lines. Read More

View Article and Full-Text PDF

Effect of Quercetin on ABCC6 Transporter: Implication in HepG2 Migration.

Int J Mol Sci 2021 Apr 8;22(8). Epub 2021 Apr 8.

Department of Sciences, University of Basilicata, 85100 Potenza, Italy.

Quercetin is a member of the flavonoid group of compounds, which is abundantly present in various dietary sources. It has excellent antioxidant properties and anti-inflammatory activity and is very effective as an anti-cancer agent against various types of tumors, both in vivo and in vitro. Quercetin has been also reported to modulate the activity of some members of the multidrug-resistance transporters family, such as P-gp, ABCC1, ABCC2, and ABCG2, and the activity of ecto-5'-nucleotidase (NT5E/CD73), a key regulator in some tumor processes such as invasion, migration, and metastasis. Read More

View Article and Full-Text PDF

Apigenin-loaded galactose tailored PLGA nanoparticles: A possible strategy for liver targeting to treat hepatocellular carcinoma.

Colloids Surf B Biointerfaces 2021 Apr 20;204:111778. Epub 2021 Apr 20.

Infectious Diseases and Immunology Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India. Electronic address:

Hepatocellular carcinoma (HCC) is the most common hepatic malignancy worldwide. Recent reports focusing on the efficacy of apigenin-loaded nanoparticles (NPs) in combating the progress of HCC encouraged us to develop galactose-tailored PLGA NPs loaded with apigenin (API-GAL-NPs) for active liver targeting to treat HCC. Two kinds of apigenin NPs, such as apigenin-PLGA NPs (API-NPs) and API-GAL-NPs were fabricated and characterized by size, surface morphology, encapsulation efficacy, and in vitro drug release kinetics. Read More

View Article and Full-Text PDF

Chemical profiling, antiviral and antiproliferative activities of the essential oil of Decne grown in Egypt.

Food Funct 2021 Apr 29. Epub 2021 Apr 29.

Pharmacognosy Department, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt and Center for Drug Discovery Research and Development, Ain Shams University, Egypt.

Here, we investigated the chemical composition of the edible Phlomis aurea oil and its anticancer potential on three human cancer cell lines, as well as its antiviral activity against Herpes simplex-1 (HSV-1). Exploring Phlomis aurea Decne essential oil by gas chromatography coupled with mass spectrometry (GC/MS) revealed the presence of four major components: germacrene D (51.56%), trans-β-farnesene (11. Read More

View Article and Full-Text PDF

New iridium bis-terpyridine complexes: synthesis, characterization, antibiofilm and anticancer potentials.

Biometals 2021 Jun 26;34(3):701-713. Epub 2021 Apr 26.

Department of Biology, Art & Science Faculty, Pamukkale University, 20160, Denizli, Turkey.

This study represents synthesis, characterization, screening of antibiofilm efficacy, and cytotoxicity of iridium bis-terpyridine complexes. The complexes were characterized by NMR, MS, FTIR, UV/Visible, and fluorescence spectroscopies. The efficacy of biofilm inhibition and eradication of iridium complexes was evaluated using a crystal violet assay test and verified by fluorescence microscopy. Read More

View Article and Full-Text PDF

Hepato-protective role of itraconazole mediated cytochrome p450 pathway inhibition in liver fibrosis.

Pak J Pharm Sci 2020 Nov;33(6(Supplementary)):2751-2758

Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Pakistan.

Liver is a vital organ and is routinely exposed to toxins. Carbon tetrachloride is one such noxious agent which cause toxicity in liver when CYP450 enzyme bio-activates it. Many hepatoprotective agents are available in market with severe side effects. Read More

View Article and Full-Text PDF
November 2020

Antiviral Activity of Interferon Alpha-Inducible Protein 27 Against Hepatitis B Virus Gene Expression and Replication.

Front Microbiol 2021 31;12:656353. Epub 2021 Mar 31.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China.

Despite the availability of effective vaccines, hepatitis B virus (HBV) is still a major health issue, and approximately 350 million people have been chronically infected with HBV throughout the world. Interferons (IFNs) are the key molecules in the innate immune response that restrict several kinds of viral infections the induction of hundreds of IFN-stimulated genes (ISGs). The objective of this study was to confirm if interferon alpha-inducible protein 27 (IFI27) as an ISG could inhibit HBV gene expression and DNA replication both in cell culture and in a mouse model. Read More

View Article and Full-Text PDF

Effect of 5-azacytidine (5-aza) on UCP2 expression in human liver and colon cancer cells.

Int J Med Sci 2021 19;18(10):2176-2186. Epub 2021 Mar 19.

College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea.

The function of the uncoupling protein 2 (UCP2) is different for each cancer cell. However, the mechanism of expression is still unclear. DNA methylation affects protein expression and is one factor that transforms normal cells into cancer cells. Read More

View Article and Full-Text PDF

Antimicrobial and anticancer properties of Carica papaya leaves derived di-methyl flubendazole mediated silver nanoparticles.

J Infect Public Health 2021 May 20;14(5):577-587. Epub 2021 Feb 20.

Department of Biotechnology, Prathyusha Engineering College, Chennai 602025, India. Electronic address:

Background: In this study, a biologically active molecule, di-methyl flubendazole isolated from the extract of Carica papaya leaves confirmed by using GC-MS, H NMR, and C NMR analysis was applied to synthesize silver nanoparticles (AgNPs). The AgNPs with plant sources an alternative therapeutic agent for synthetic compound used in cancer chemotherapy.

Methods: The AgNPs were characterized using UV, FT-IR, XRD, FESEM with EDX and TEM. Read More

View Article and Full-Text PDF

Microindoline 581, an Indole Derivative from Sp. RP581 as A Novel Selective Antineoplastic Agent to Combat Hepatic Cancer Cells: Production, Optimization and Structural Elucidation.

Iran J Pharm Res 2020 ;19(4):290-305

Molecular Biotechnology Laboratory, Department of Biology, Faculty of Science, Shiraz University, Iran.

Screening of bioactive compounds with potential binding affinity to DNA as one of the target molecules in fighting against cancer cells has gained the attention of many scientists. Finding such compounds in the cellular content of microorganisms, especially marine bacteria as valuable and rich natural resources, is of great importance. sp. Read More

View Article and Full-Text PDF
January 2020

Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From SN-8.

Front Microbiol 2021 26;12:644226. Epub 2021 Mar 26.

College of Food Science and Engineering, Ocean University of China, Qingdao, China.

In this study, a novel exopolysaccharide (EPS) was extracted from Shen Nong's (SN)-8 which can be obtained from Dajiang. After the purification step, EPS-8-2 was obtained with molecular weights of 1.46 × 10 Da. Read More

View Article and Full-Text PDF

Herbal formula of Bushen Jianpi combined with sorafenib inhibits hepatocellular carcinoma growth by promoting cell apoptosis and blocking the cell cycle.

J Tradit Chin Med 2021 04;41(2):194-202

Oncology Department, Shanghai TCM-Integrated Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China.

Objective: To investigate the efficacy of an herbal formula of Bushen Jianpi ( BSJP) combined with sorafenib on hepatocellular carcinoma (HCC) in vitro and in vivo, and to study the underlying mechanisms of action.

Methods: BSJP, a mixture of 12 raw herbs, was extracted in 70% alcohol/30% water and freeze-dried into a powder. The in vitro effects of BSJP alone, sorafenib alone, and their combination on cell survival, apoptosis, and cell cycle distribution were evaluated in HCC cell lines HCCLM3, HepG2, and SMMC-7721. Read More

View Article and Full-Text PDF

Effects of fetuin-A-containing calciprotein particles on posttranslational modifications of fetuin-A in HepG2 cells.

Sci Rep 2021 Apr 5;11(1):7486. Epub 2021 Apr 5.

Department of Metabolism, Endocrinology and Molecular Medicine, Osaka City University Graduate School of Medicine, Osaka, Japan.

Fetuin-A is an inhibitor of ectopic calcification that is expressed mainly in hepatocytes and is secreted into the circulation after posttranslational processing, including glycosylation and phosphorylation. The molecular weight (MW) of fully modified fetuin-A (FM-fetuin-A) is approximately 60 kDa in an immunoblot, which is much higher than the estimated MW by amino acid sequence. Under conditions of calcification stress such as advanced stage chronic kidney disease, fetuin-A prevents calcification by forming colloidal complexes, which are referred to as calciprotein particles (CPP). Read More

View Article and Full-Text PDF

Synthesis and Characterization of a Minophosphonate Containing Chitosan Polymer Derivatives: Investigations of Cytotoxic Activity and in Silico Study of SARS-CoV-19.

Polymers (Basel) 2021 Mar 26;13(7). Epub 2021 Mar 26.

PG & Research, Department of Chemistry, Nehru Memorial College, Affiliated Bharathidasan University, Puthanamapatti, Tamilnadu 621007, India.

Chitosan is broadly used as a biological material since of its excellent biological activities. This work describes investigations of chitosan interaction with SARS-CoV-2, which is occupied by human respiratory epithelial cells through communication with the human angiotension-converting enzyme II (ACE2). The β-chitosan derivatives are synthesized and characterized by FT-IR, nuclear magnetic resonance (H and C NMR), mass spectrometry, X-ray diffraction, TGA, DSC, and elemental analysis. Read More

View Article and Full-Text PDF

Design, Synthesis, Molecular Modeling and Antitumor Evaluation of Novel Indolyl-Pyrimidine Derivatives with EGFR Inhibitory Activity.

Molecules 2021 Mar 25;26(7). Epub 2021 Mar 25.

Pharmaceutical Organic Chemistry Department, Faculty of Pharmacy, Helwan University, Ein-Helwan, Helwan, Cairo 11795, Egypt.

Scaffolds hybridization is a well-known drug design strategy for antitumor agents. Herein, series of novel indolyl-pyrimidine hybrids were synthesized and evaluated in vitro and in vivo for their antitumor activity. The in vitro antiproliferative activity of all compounds was obtained against MCF-7, HepG2, and HCT-116 cancer cell lines, as well as against WI38 normal cells using the resazurin assay. Read More

View Article and Full-Text PDF

Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury.

Molecules 2021 Mar 15;26(6). Epub 2021 Mar 15.

Research Institute, Ato Q&A Co., LTD, Jeonju-si 55069, Korea.

, also known as Kushen, has traditionally been used as a herbal medicine. In the present study we evaluated the ameliorative effects of kushenol C (KC) from against tBHP (tert-Butyl hydroperoxide)-induced oxidative stress in hepatocellular carcinoma (HEPG2) cells and acetaminophen (APAP)-induced hepatotoxicity in mice. KC pretreatment protected the HEPG2 cells against oxidative stress by reducing cell death, apoptosis and reactive oxygen species (ROS) generation. Read More

View Article and Full-Text PDF

Combining combretastatin A4 phosphate with ginsenoside Rd synergistically inhibited hepatocellular carcinoma by reducing HIF-1α via PI3K/AKT/mTOR signalling pathway.

J Pharm Pharmacol 2021 Mar;73(2):263-271

Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, P.R. China.

Objectives: Combretastatin A4 phosphate (CA4P), a vascular disrupting agent (VDA), can cause rapid tumour vessel occlusion. Subsequently, extensive necrosis is discovered in the tumour center, which induces widespread hypoxia and the rise of the α subunit of hypoxia-inducible factor-1 (HIF-1α). The aim of this study was to evaluate the inhibition of hepatocellular carcinoma growth by combining CA4P with HIF-1 α inhibitor and investigate the mechanism of this combination. Read More

View Article and Full-Text PDF

Anti-inflammatory Effects of Alcohol Are Associated with JNK-STAT3 Downregulation in an Inflammation Model in HepG2 Cells.

Dis Markers 2021 18;2021:6622701. Epub 2021 Mar 18.

Department of Trauma, Hand and Reconstructive Surgery, University Hospital Frankfurt, Goethe University, Frankfurt am Main, Germany.

Background: In several preclinical and models of acute inflammation, alcohol (ethanol, EtOH) has been described as an immunomodulatory agent. Similarly, in different pathologies, clinical observations have confirmed either pro- or anti-inflammatory effects of EtOH. The liver plays an important role in immunity and alcohol metabolism; therefore, we analysed dose- and time-dependent effects of EtOH on the inflammatory response of human liver cells in an model of acute inflammation. Read More

View Article and Full-Text PDF

Effects of anesthetic agents on inflammation in Caco-2, HK-2 and HepG2 cells.

Exp Ther Med 2021 May 16;21(5):487. Epub 2021 Mar 16.

Department of Anesthesiology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China.

Anesthetic agents are often used in surgical procedures to relieve pain in patients with traumatic injuries. Several anesthetic agents can cause immunosuppression by suppressing the secretion of immune factors such as cytokines. However, the effects of different anesthetic agents on inflammation are not completely understood. Read More

View Article and Full-Text PDF

Hepatitis B virus induces sorafenib resistance in liver cancer via upregulation of cIAP2 expression.

Infect Agent Cancer 2021 Mar 23;16(1):20. Epub 2021 Mar 23.

Department of General Surgery, Second Affiliated Hospital of Nanchang University, 1 Minde Road, Nanchang, Jiangxi, China.

Background: HBV promotes cell survival by upregulating the expression of the cellular inhibitor of apoptosis protein 2 (cIAP2), however whether it is involved in HBV-induced sorafenib resistance in liver cancer remains unclear.

Methods: cIAP2 overexpression and knockdown was adopted to assess the involvement of cIAP2 in HBV-induced sorafenib resistance. Anti-HBV drug lamivudine and Akt inhibitor were used to investigate the impact of HBV replication on cIAP2 expression and sorafenib resistance. Read More

View Article and Full-Text PDF

Design and synthesis of diosgenin derivatives as apoptosis inducers through mitochondria-related pathways.

Eur J Med Chem 2021 May 10;217:113361. Epub 2021 Mar 10.

College of Pharmacy, Qiqihar Medical University, Qiqihar, 161006, Heilongjiang, PR China. Electronic address:

Diosgenin (DSG) has attracted attention recently as a potential anticancer therapeutic agent due to its profound antitumor activity. To better utilize DSG as an antitumor compound, two series of DSG-amino acid ester derivatives (3a-3g and 7a-7g) were designed and synthesized, and their cytotoxic activities against six human cancer cell lines (K562, T24, MNK45, HepG2, A549, and MCF-7) were evaluated. The results obtained showed that a majority of derivatives exhibited cytotoxic activities against these six human tumor cells. Read More

View Article and Full-Text PDF

Cytotoxic Effect of Bromelain on HepG2 Hepatocellular Carcinoma Cell Line.

Appl Biochem Biotechnol 2021 Mar 18. Epub 2021 Mar 18.

Department of Chemical Engineering, JNTUA College of Engineering, Ananthapuram, 515002, Andhra Pradesh, India.

Cancer is a complicated long-term disease due to computable key molecular players involved in aggravating the disease. Among various kinds of cancer, hepatocellular carcinoma (HCC) is the ninth leading cause of cancer. Recently, plant-based products are gaining a lot of attention in the field of research because of their anti-tumor properties. Read More

View Article and Full-Text PDF

Preventive activity of Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in and models.

J Toxicol Environ Health A 2021 Mar 17:1-13. Epub 2021 Mar 17.

Laboratório De Mutagênese, Universidade De Franca, Franca, São Paulo, Brazil.

Desf. is a plant found in South America, especially in Brazil. Oleoresin and the leaves of this plant is used as a popular medicinal agent. Read More

View Article and Full-Text PDF

Antisense microRNA185 loaded liposome for efficient inhibition of the hepatic endogenous microRNA185 level.

Eur J Pharm Sci 2021 Jun 13;161:105803. Epub 2021 Mar 13.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China; NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050, China. Electronic address:

MicroRNA185 (miR185), an endogenous noncoding RNA with 23 nucleotides, is one of key posttranscriptional modulators of cholesterol metabolism in hepatic cells. The antisense inhibitor of miR185 (miR185i) could decrease cholesterol level in vivo, providing a promising agent for anti-atherosclerosis strategy. In this work, a novel LipomiR185i was constructed by thin film hydration method and post-PEGylation as DOPE: DOTAP: Chol: DSPE-PEG at the molar ratio of 1:1:1:0. Read More

View Article and Full-Text PDF