62,020 results match your criteria affect binding

A novel and potent thrombolytic fusion protein consisting of anti-insoluble fibrin antibody and mutated urokinase.

Thromb Haemost 2021 Apr 21. Epub 2021 Apr 21.

National Cancer Center Research Institute, Immune Medicine, Tokyo, Japan.

Tissue plasminogen activator (tPA) is used clinically because it has higher binding specificity for insoluble fibrin (IF) than urokinase (UK), but even pro-tPA has catalytic activity in places other than IF. UK has the advantage that it is specifically activated on IF, but it binds IF weakly. Previously, we established a monoclonal antibody (mAb) that recognizes a pit structure formed only in IF. Read More

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Single nucleotide polymorphisms affect miRNA target prediction in bovine.

PLoS One 2021 21;16(4):e0249406. Epub 2021 Apr 21.

Department of Production and Animal Health, São Paulo State University (Unesp), School of Veterinary Medicine, Araçatuba, São Paulo, Brazil.

Single nucleotide polymorphisms (SNPs) can have significant effects on phenotypic characteristics in cattle. MicroRNAs (miRNAs) are small, non-coding RNAs that act as post-transcriptional regulators by binding them to target mRNAs. In the present study, we scanned ~56 million SNPs against 1,064 bovine miRNA sequences and analyzed, in silico, their possible effects on target binding prediction, primary miRNA formation, association with QTL regions and the evolutionary conservation for each SNP locus. Read More

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Insights into the mechanism of shortened developmental duration and accelerated weight gain associated with Wolbachia infection in Hylyphantes graminicola.

Integr Zool 2021 Apr 21. Epub 2021 Apr 21.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.

Wolbachia infection is known to affect host reproduction and development. To date, however, the underlying mechanism related to the effects of Wolbachia on host development has not yet been reported. Here, we compared the developmental duration and body weight in different instars of Wolbachia-positive (W ) and Wolbachia-negative (W ) spiders (Hylyphantes graminicola) and detected the relative expression levels of six insulin-related genes and three ecdysone-related genes using reverse transcription qPCR (RT-qPCR). Read More

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Rotational Dynamics of Water at the Phospholipid Bilayer Depending on the Head Groups Studied by Molecular Dynamics Simulations.

Langmuir 2021 Apr 21. Epub 2021 Apr 21.

Department of Chemistry, Faculty of Pure and Applied Sciences, University of Tsukuba, Tsukuba 305-8571, Ibaraki, Japan.

Hydration states are a crucial factor that affect the self-assembly and properties of soft materials and biomolecules. Although previous experiments have revealed that the hydration state strongly depends on the chemical structure of lipid molecules, the mechanisms at the molecular level remain unknown. Classical and density-functional tight-binding (DFTB) molecular dynamics (MD) simulations are employed to determine the mechanisms underlying dissimilar water dynamics between lipid membranes with phosphatidylcholine (PC) and phosphatidylethanolamine (PE) head groups. Read More

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A multidrug resistance-associated protein inhibitor is a potential enhancer of the benzyl isothiocyanate-induced apoptosis induction in human colorectal cancer cells.

J Biochem Mol Toxicol 2021 Apr 21:e22791. Epub 2021 Apr 21.

Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.

The increasing drug efflux through the ATP-binding cassette (ABC) transporters is the most plausible mechanism that mediates resistance to the anticancer phytochemicals, such as benzyl isothiocyanate (BITC), as well as chemotherapy drugs. To identify a potential component to overcome this resistance by combinatory utilization, we focused on multidrug resistance-associated proteins (MRPs) pumping various drug metabolites with glutathione as well as the organic anions. The pharmacological treatment of an MRP inhibitor, MK571, significantly potentiated the BITC-induced antiproliferation, coincided with the enhanced accumulation of BITC and glutathione in human colorectal cancer HCT-116 cells. Read More

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Homophilic and heterophilic cadherin bond rupture forces in homo- or hetero-cellular systems measured by AFM-based single-cell force spectroscopy.

Eur Biophys J 2021 Apr 20. Epub 2021 Apr 20.

Institute of Biophysics, University of Bremen, Bremen, Germany.

Cadherins enable intercellular adherens junctions to withstand tensile forces in tissues, e.g. generated by intracellular actomyosin contraction. Read More

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Amino Acid Digestibility and Digestible Indispensable Amino Acid Score (DIAAS)-Like Values of Black Soldier Fly Larvae Fed Different Forms and Concentrations of Calcium Using the Precision-Fed Cecectomized Rooster Assay.

J Anim Sci 2021 Apr 21. Epub 2021 Apr 21.

Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Black soldier fly larvae (BSFL) is an alternative protein source for animals, including dogs and cats. Dietary calcium source is an essential nutrient for BSFL development in the pupal stage. Calcium carbonate (CaCO3) and calcium chloride (CaCl2) are common calcium sources, but differ in solubility, acid-binding capacity, and calcium concentration. Read More

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Revealing the threat of emerging SARS-CoV-2 mutations to antibody therapies.

bioRxiv 2021 Apr 12. Epub 2021 Apr 12.

The ongoing massive vaccination and the development of effective intervention offer the long-awaited hope to end the global rage of the COVID-19 pandemic. However, the rapidly growing SARS-CoV-2 variants might compromise existing vaccines and monoclonal antibody (mAb) therapies. Although there are valuable experimental studies about the potential threats from emerging variants, the results are limited to a handful of mutations and Eli Lilly and Regeneron mAbs. Read More

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A G protein-coupled receptor-like module regulates cellulose synthase secretion from the endomembrane system in Arabidopsis.

Dev Cell 2021 Apr 13. Epub 2021 Apr 13.

School of Biosciences, University of Melbourne, Parkville 3010 VIC, Australia; Max-Planck Institute for Molecular Plant Physiology, Am Muehlenberg 1, 14476 Potsdam, Germany; Department of Plant & Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark; Copenhagen Plant Science Center, University of Copenhagen, 1871 Frederiksberg C, Denmark; Joint International Research Laboratory of Metabolic & Developmental Sciences, State Key Laboratory of Hybrid Rice, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Cellulose is produced at the plasma membrane of plant cells by cellulose synthase (CESA) complexes (CSCs). CSCs are assembled in the endomembrane system and then trafficked to the plasma membrane. Because CESAs are only active in the plasma membrane, control of CSC secretion regulates cellulose synthesis. Read More

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RNA-Sequencing Analysis of Paternal Low-Protein Diet-Induced Gene Expression Change in Mouse Offspring Adipocytes.

G3 (Bethesda) 2019 Jul;9(7):2161-2170

RIKEN Cluster for Pioneering Research, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.

Increasing evidence indicates that parental diet affects the metabolism and health of offspring. It is reported that paternal low-protein diet (pLPD) induces glucose intolerance and the expression of genes involved in cholesterol biosynthesis in mouse offspring liver. The aim of the present study was to determine the effect of a pLPD on gene expression in offspring white adipose tissue (WAT), another important tissue for the regulation of metabolism. Read More

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Control of guest binding behavior of metal-containing host molecules by ligand exchange.

Shigehisa Akine

Dalton Trans 2021 Apr 23;50(13):4429-4444. Epub 2021 Mar 23.

Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.

This review describes the control of guest binding behavior of metal-containing host molecules that is driven by ligand exchange reactions at the metal centers. Recently, a vast number of metal-containing host molecules including metal-assisted self-assembled structures have been developed, and the structural transformation after construction of the host framework has now been of interest from the viewpoint of functional switching and tuning. Among the various kinds of chemical transformations, ligand exchange has a great advantage in the structural conversions of metal-containing hosts, because ligand exchange usually proceeds under mild conditions that do not affect the host framework. Read More

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Role of ERβ in Adipocyte Metabolic Response to Wheel Running Following Ovariectomy.

J Endocrinol 2021 Apr 1. Epub 2021 Apr 1.

V Vieira-Potter, Department of Nutrition and Exercise Physiology, University of Missouri System, Columbia, United States.

Estrogen receptor  (ERb), one of the two major estrogen receptors, acts via genomic and non-genomic signaling pathways to affect many metabolic functions, including mitochondrial biogenesis and respiration. This study assessed the effect of ERb classical genomic activity on adipocyte-specific and systemic metabolic responses to wheel running exercise in a rodent model of menopause. Female mice lacking the ERb DNA-binding domain (ERbDBDKO, N = 20) and wild-type (WT, N = 21) littermate controls were fed high-fat diet (HFD), ovariectomized (OVX), and randomized to control (no running wheel) and exercise (running wheel access) groups and were followed for eight weeks. Read More

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Computational study to evaluate the potency of phytochemicals in and the impact of point mutation on cyclin-dependent kinase 2-associated protein 1.

J Biomol Struct Dyn 2021 Apr 20:1-15. Epub 2021 Apr 20.

Department of Chemistry, National Institute of Technology, Raipur, India.

A protein's function is closely related to its structural properties. Mutations can affect the functionality of a protein. Different cancer tissues have found disordered expression of the cyclin-dependent kinase 2-associated Protein 1 (CDK2AP1) gene. Read More

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The Interplay Between Postsynaptic Striatal D2/3 Receptor Availability, Adversity Exposure and Odd Beliefs: A [11C]-Raclopride PET Study.

Schizophr Bull 2021 Apr 20. Epub 2021 Apr 20.

Department of Psychiatry, Psychotherapy and Psychosomatics, Psychiatric University Hospital Zurich, Zurich Program for Sustainable Development of Mental Health Services (ZInEP), University of Zurich, Zurich, Switzerland.

Background: Between unaffected mental health and diagnosable psychiatric disorders, there is a vast continuum of functioning. The hypothesized link between striatal dopamine signaling and psychosis has guided a prolific body of research. However, it has been understudied in the context of multiple interacting factors, subclinical phenotypes, and pre-postsynaptic dynamics. Read More

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Endocrine toxicities of immune checkpoint inhibitors.

Nat Rev Endocrinol 2021 Apr 19. Epub 2021 Apr 19.

Department of Medicine, Vanderbilt University Medical Center and Vanderbilt Ingram Cancer Center, Nashville, TN, USA.

Immune checkpoint inhibitors (ICIs) are monoclonal antibodies that target two key signalling pathways related to T cell activation and exhaustion, by binding to and inhibiting cytotoxic T lymphocyte antigen 4 (CTLA4) or PD1 and its ligand PDL1. ICIs, such as nivolumab, pembrolizumab and ipilimumab, are approved for the treatment of numerous and diverse cancer types, in various combination regimens, and are now an established cornerstone of cancer therapeutics. Toxicities induced by ICIs are autoimmune in nature and are referred to as immune-related adverse events (irAEs); these events can affect any organ system in an unpredictable fashion. Read More

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In silico drug repurposing for filarial infection predicts nilotinib and paritaprevir as potential inhibitors of the Wolbachia 5'-aminolevulinic acid synthase.

Sci Rep 2021 Apr 19;11(1):8455. Epub 2021 Apr 19.

Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, KNUST, Kumasi, Ghana.

Filarial infections affect millions of individuals and are responsible for some notorious disabilities. Current treatment options involve repeated mass drug administrations, which have been met with several challenges despite some successes. Administration of doxycycline, an anti-Wolbachia agent, has shown clinical effectiveness but has several limitations, including long treatment durations and contraindications. Read More

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Genetic variants of the EGFR ligand-binding domain and their association with structural alterations in Arab cancer patients.

BMC Res Notes 2021 Apr 19;14(1):146. Epub 2021 Apr 19.

Department of Life Sciences, Health Biotechnology Program, College of Graduate Studies, Arabian Gulf University, PO Box 26671, Manama, Kingdom of Bahrain.

Objective: This study aimed to identify novel genetic variants in the CR2 extracellular domain of the epidermal growth factor receptor (EGFR) in healthy individuals and patients with six different types of adenocarcinoma, in Arabian peninsula populations. It also aimed to investigate the effects of these variants on the EGFR structure and their eventual relevance to tumorigenesis.

Results: We detected seven new EGFR genetic variants in 168 cancer patients and 114 controls. Read More

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The Role of Cation Diffusion Facilitator CDF-1 in Lipid Metabolism in Caenorhabditis elegans.

G3 (Bethesda) 2021 Apr 19. Epub 2021 Apr 19.

Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming, Yunnan 650091, China.

Zinc is one of the most important trace elements as it plays a vital role in many biological processes. As well, aberrant zinc metabolism has been implicated in lipid-related metabolic diseases. Previously, we showed that zinc antagonizes iron to regulate sterol regulatory element-binding proteins and the stearoyl-CoA desaturase (SREBP-SCD) pathway in lipid metabolism in the model organism Caenorhabditis elegans. Read More

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CDK14/β-catenin/TCF4/miR-26b positive feedback regulation modulating pancreatic cancer cell phenotypes in vitro and tumor growth in mice model in vivo.

J Gene Med 2021 Apr 19:e3343. Epub 2021 Apr 19.

Department of Post-anesthetic ICU, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Introduction: Chemotherapy and radiotherapy have been reported to be basically ineffective for pancreatic ductal adenocarcinoma (PDAC) patients; thus, gene therapy might provide a novel approach for them. CDK14, a new oncogenic member of CDK family involving in pancreatic cancer cell response to Gemcitabine (GEM) treatment, has been reported to be regulated by miRNAs. In this study, we aimed to investigate whether miR-26b regulated CDK14 expression to affect the phenotype of pancreatic cancer cells. Read More

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Ligand Binding Prolongs Androgen Receptor Protein Half-Life by Reducing its Degradation.

J Endocr Soc 2021 May 5;5(5):bvab035. Epub 2021 Mar 5.

Division of Endocrinology and Metabolism, Department of Medicine, University of Rochester Medical Center, Rochester, New York 14642, USA.

Androgens are important in female reproduction, but the molecular actions of androgens in female reproductive tissues are not fully understood. We investigated the androgen-responsive transcriptome in human and mouse granulosa cells (GCs) and surprisingly found that the gene-regulation activity of androgen receptor (AR) in these cells is negligible. We then investigated extranuclear actions of AR and found that in human and mouse GCs, as well as in prostate cancer cells, dihydrotestosterone (DHT) dramatically increases the half-life of its own receptor protein. Read More

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Advancing peptide siRNA-carrier designs through L/D-amino acid stereochemical modifications to enhance gene silencing.

Mol Ther Nucleic Acids 2021 Jun 19;24:462-476. Epub 2021 Mar 19.

Department of Oral Health Sciences, James B. Edwards College of Dental Medicine, Medical University of South Carolina (MUSC), Charleston, SC 29425, USA.

The 599 peptide has been previously shown to effectively deliver small interfering RNAs (siRNAs) to cancer cells, inducing targeted-oncogene silencing, with a consequent inhibition of tumor growth. Although effective, this study was undertaken to advance the 599 peptide siRNA-carrier design through L/D-amino acid stereochemical modifications. Consequently, 599 was modified to generate eight different peptide variants, incorporating either different stereochemical patterns of L/D-amino acids or a specific D-amino acid substitution. Read More

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Role of Polymorphisms of NKG2D Receptor and Its Ligands in Acute Myeloid Leukemia and Human Stem Cell Transplantation.

Front Immunol 2021 30;12:651751. Epub 2021 Mar 30.

Laboratory of Tumor Biology and Immunotherapy, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czechia.

Natural killer cells possess key regulatory function in various malignant diseases, including acute myeloid leukemia. NK cell activity is driven by signals received through ligands binding activating or inhibitory receptors. Their activity towards elimination of transformed or virally infected cells can be mediated through MICA, MICB and ULBP ligands binding the activating receptor NKG2D. Read More

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PMMA-Based Continuous Hemofiltration Modulated Complement Activation and Renal Dysfunction in LPS-Induced Acute Kidney Injury.

Front Immunol 2021 1;12:605212. Epub 2021 Apr 1.

Nephrology, Dialysis and Transplantation Unit, Department of Medical and Surgical Science, University of Foggia, Foggia, Italy.

Sepsis-induced acute kidney injury (AKI) is a frequent complication in critically ill patients, refractory to conventional treatments. Aberrant activation of innate immune system may affect organ damage with poor prognosis for septic patients. Here, we investigated the efficacy of polymethyl methacrylate membrane (PMMA)-based continuous hemofiltration (CVVH) in modulating systemic and tissue immune activation in a swine model of LPS-induced AKI. Read More

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Towards a Functional Cure of HIV-1: Insight Into the Chromatin Landscape of the Provirus.

Front Microbiol 2021 18;12:636642. Epub 2021 Mar 18.

Laboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Leuven, Belgium.

Despite potent combination antiretroviral therapy, HIV-1 infection persists due to irreversible integration of the virus in long-living cells of the immune system. The main focus of HIV-1 cure strategies has been on HIV-1 eradication, yet without great success so far. Therefore, HIV-1 remission or a functional cure, whereby the virus is silenced rather than eradicated, is considered as an alternative strategy. Read More

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Quantitative Chemical Proteomics Reveals Resveratrol Inhibition of A549 Cell Migration Through Binding Multiple Targets to Regulate Cytoskeletal Remodeling and Suppress EMT.

Front Pharmacol 2021 26;12:636213. Epub 2021 Mar 26.

School of Medicine and Holistic Integrative Medicine and College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.

Resveratrol (RSV), a health-promoting natural product, has been shown to affect various cellular processes in tumor cells. However, the specific protein targets of RSV and the mechanism of action (MOA) of its anticancer effect remain elusive. In this study, the pharmacological activity of RSV was first evaluated in A549 cells, and the results showed that RSV significantly inhibited A549 cell migration but did not affect cell viability. Read More

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Epigenetics drive the evolution of sex chromosomes in animals and plants.

Philos Trans R Soc Lond B Biol Sci 2021 Jun 19;376(1826):20200124. Epub 2021 Apr 19.

The Francis Crick institute, London, UK.

We review how epigenetics affect sex chromosome evolution in animals and plants. In a few species, sex is determined epigenetically through the action of Y-encoded small RNAs. Epigenetics is also responsible for changing the sex of individuals through time, even in species that carry sex chromosomes, and could favour species adaptation through breeding system plasticity. Read More

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Interaction of pharmaceutical metabolites with blood proteins and membrane lipids in the view of bioconcentration: A preliminary study based on in vitro assessment.

Sci Total Environ 2021 Apr 9;783:146987. Epub 2021 Apr 9.

Department of Environmental Analysis, Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland.

Pharmaceuticals pose a real threat to the environment, which has been proven in many studies to date. However, still little is known about the transformation products (TPs) of these compounds, which can also interact with organisms, causing adverse effects like noticeable toxicity or bioconcentration. Many recent works confirm that metabolites of pharmaceuticals are present in the environment, and preliminary studies suggest that they may be equally dangerous to or even more so than their parent compounds. Read More

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MicroRNAs and angiogenesis: a new era for the management of colorectal cancer.

Cancer Cell Int 2021 Apr 17;21(1):221. Epub 2021 Apr 17.

Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200071, China.

MicroRNAs (miRNAs) are a class of small noncoding RNA molecules containing only 20-22 nucleotides. MiRNAs play a role in gene silencing and translation suppression by targeting and binding to mRNA. Proper control of miRNA expression is very important for maintaining a normal physiological environment because miRNAs can affect most cellular pathways, including cell cycle checkpoint, cell proliferation, and apoptosis pathways, and have a wide range of target genes. Read More

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Osteocalcin promotes proliferation, differentiation, and survival of PC12 cells.

Biochem Biophys Res Commun 2021 Apr 14;557:174-179. Epub 2021 Apr 14.

Division of Applied Pharmacology, Department of Health Promotion, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu 803-8580, Japan. Electronic address:

Involvement of the bone matrix protein osteocalcin (OC) in the development of learning and memory, and the prevention of anxiety-like behaviors in mice. However, the direct effects of OC on neurons are still unknown comparing to the mechanism how OC affects systemic energy expenditure and glucose homeostasis. In this study, we investigated the effect of OC on proliferation, differentiation, and survival of neurons using the rat pheochromocytoma cell line PC12. Read More

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Role of IGFBP-2 in oral cancer metastasis.

Biochim Biophys Acta Mol Basis Dis 2021 Apr 14:166143. Epub 2021 Apr 14.

Department of Medical Science, Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan. Electronic address:

Cancer metastasis is one of most main causes of failure in cancer treatment. Nonetheless, more than half of oral cancer patients were diagnosed as advanced oral cancer with dramatically decreased 5-year survival rate to lower than 20%, while the stages become more advanced. In order to improve oral cancer treatment, the identification of cancer metastatic biomarkers and mechanisms is critical. Read More

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