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Urban-Rural Compliance Variability to COVID-19 Restrictions of Indigenous Fijian (iTaukei) Funerals in Fiji.

Authors:
Ron Vave

Asia Pac J Public Health 2021 Apr 14:10105395211005921. Epub 2021 Apr 14.

University of Hawai'i at Mānoa, Honolulu, HI, USA.

Research on coronavirus disease 2019 (COVID-19) has focused primarily on impacts in Western societies despite emerging evidence of increased vulnerability among indigenous peoples such as Pacific Islanders. Using Facebook public posts, this research assessed compliance to COVID-19 restrictions such as social gatherings (SG) and social distancing (SD) in non-COVID-19, indigenous Fijian (iTaukei) funerals in Fiji. Results showed 95% of the 20 funerals exceeding SG limits with greater, and highly variable crowd sizes in rural than urban communities. Read More

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Effect of Low-Intensity vs High-Intensity Home-Based Walking Exercise on Walk Distance in Patients With Peripheral Artery Disease: The LITE Randomized Clinical Trial.

JAMA 2021 04;325(13):1266-1276

Wake Forest University, Winston-Salem, North Carolina.

Importance: Supervised high-intensity walking exercise that induces ischemic leg symptoms is the first-line therapy for people with lower-extremity peripheral artery disease (PAD), but adherence is poor.

Objective: To determine whether low-intensity home-based walking exercise at a comfortable pace significantly improves walking ability in people with PAD vs high-intensity home-based walking exercise that induces ischemic leg symptoms and vs a nonexercise control.

Design, Setting, And Participants: Multicenter randomized clinical trial conducted at 4 US centers and including 305 participants. Read More

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Binding of collagen gene products with titanium oxide.

J Biochem 2021 Feb 25. Epub 2021 Feb 25.

Faculty of Environmental Earth Science, Hokkaido University, Sapporo Japan 060-0810.

Titanium is the only metal to which osteoblasts can adhere and on which they can grow and form bone tissue in vivo, resulting in a strong bond between the implant and living bone. This discovery provides the basis for the universal medical application of Ti. However, the biochemical mechanism of bond formation is still unknown. Read More

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February 2021

Development of NaCoO Thick Film Prepared by Screen-Printing Process.

Materials (Basel) 2020 Jun 22;13(12). Epub 2020 Jun 22.

National Institute of Advanced Industrial Science and Technology (AIST), Shimo-Shidami, Moriyama-ku, Nagoya 463-8560, Japan.

The NaCoCuO thick film with the same thermoelectric performance as a NaCoO bulk was formed on an alumina substrate by the screen-printing process. The power factor exceeded 0.3 mW/Km, with the resistivity of 3. Read More

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Proteomic analysis of bone proteins adsorbed onto the surface of titanium dioxide.

Biochem Biophys Rep 2016 Sep 16;7:316-322. Epub 2016 Jul 16.

Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.

Osseointegration is the structural and functional connection between bone tissues and implants such as titanium dioxide (TiO). The bone-TiO interface is thought to contain proteoglycans. However, exhaustive analysis of the proteins in this layer has not been performed. Read More

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September 2016

Physical activity as a vital sign in patients with schizophrenia: Evidence and clinical recommendations.

Schizophr Res 2016 Feb 11;170(2-3):336-40. Epub 2016 Jan 11.

School of Psychiatry, University of New South Wales, Sydney, Australia.

Aim: This study investigated whether in patients with schizophrenia the Physical Activity Vital Sign (PAVS) assessment can identify those at higher risk of cardio-metabolic abnormalities.

Methods: One hundred patients with schizophrenia completed the PAVS-questions exploring whether they adhered to the general physical activity recommendations, underwent a full-fasting metabolic screening, and performed a six-minute walk test (6 MWT).

Results: Those who do not adhere to the health recommendations according to the PAVS (n=61) were significantly older (37. Read More

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February 2016

Inactivation of viruses using novel protein A wash buffers.

Biotechnol Prog 2015 Mar-Apr;31(2):406-13. Epub 2014 Dec 19.

Biogen, Cambridge, MA, 02142.

Low pH viral inactivation is typically performed in the eluate pool following the protein A capture step during the manufacturing of monoclonal antibodies and Fc-fusion proteins. However, exposure to low pH has the potential to alter protein quality. To avoid these difficulties, novel wash buffers capable of inactivating viruses while antibodies or Fc-fusion proteins were bound to protein A or mixed mode resins were developed. Read More

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January 2016

A container closure system that allows for greater recovery of radiolabeled peptide compared to the standard borosilicate glass system.

Appl Radiat Isot 2013 Oct 24;80:99-102. Epub 2013 Jun 24.

Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA.

Objectives: Often peptides used in synthesis of radiopharmaceutical PET tracers are lipophilic and adhere to the walls of container closure systems (CCS) such that costly peptide and product are not fully recoverable after synthesis occurs. This investigation compares a standard United States Pharmacopeia (USP) Type I borosilicate glass CCS to a cyclic polyolefin copolymer Crystal Zenith(®) (CZ) CCS, for (68)Ga-chloride and (68)Ga-DOTATOC ([(68)Ga] Ga-DOTA-D-Phe1-Tyr3-octreotide) retention in the reaction vial after labeling.

Methods: (68)Gallium labeling of DOTATOC was conducted by adding (68)Ga-chloride, 2M HEPES (4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid) or 0. Read More

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October 2013

Outbreak of Ralstonia solanacearum Biovar 2 Causing Brown Rot on Potato in the Aegean Region of Turkey.

Plant Dis 2008 Jun;92(6):973

Plant Protection Central Research Institute, Ankara, Turkey.

Ralstonia solanacearum (Smith) Yabuuchi, Kosako, Yano, Hotta, and Nishiuchi, the cause of brown rot of potato (Solanum tuberosum), was detected for the first time in Turkey in 1995 in five potato fields in the Nevsehir Province of the central Anatolia Region and was eradicated under measures mandated by the government. Occurrence of the pathogen was not reported in other parts of the country. However, in 2006, brown rot symptoms were observed in potato (cv. Read More

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The effect of prebiotics on production of antimicrobial compounds, resistance to growth at low pH and in the presence of bile, and adhesion of probiotic cells to intestinal mucus.

J Appl Microbiol 2006 Apr;100(4):813-20

Department of Physiological Sciences, University of Stellenbosch, Stellenbosch, South Africa.

Aims: Screening of five bile salt-resistant and low pH-tolerant lactic acid bacteria for inhibitory activity against lactic acid bacteria and bacterial strains isolated from the faeces of children with HIV/AIDS. Determining the effect of prebiotics and soy milk-base on cell viability and adhesion of cells to intestinal mucus.

Methods And Results: Lactobacillus plantarum 423, Lactobacillus casei LHS, Lactobacillus salivarius 241, Lactobacillus curvatus DF 38 and Pediococcus pentosaceus 34 produced the highest level of antimicrobial activity (12,800 AU ml(-1)) when grown in MRS broth supplemented with 2% (m/v) dextrose. Read More

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Effects of negatively charged groups (carboxymethyl) on the calcification of poly(2-hydroxyethyl methacrylate).

Biomaterials 2002 Jul;23(14):3053-9

LHEA-GEROM, Laboratoire d'Histologie-Embryologie, Faculté de Médecine & CHU d'Angers, France.

Poly(2-hydroxyethyl methacrylate) (pHEMA) has potentially wide biomedical applications: it is biocompatible, allows immobilization of cells or bioactive molecules and has a hardness comparable to bone. We previously reported that immobilization of alkaline phosphatase (AlkP) in pHEMA can initiate mineralization in a manner that mimics the calcification of cartilage and woven bone. Because numerous proteins known to initiate mineralization possess acidic species, we have modified the neutral electrical surface of pHEMA by carboxymethylation (CM). Read More

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A Collagen-Binding S-Layer Protein in Lactobacillus crispatus.

Appl Environ Microbiol 1995 Jul;61(7):2467-71

Two S-layer-expressing strains, Lactobacillus crispatus JCM 5810 and Lactobacillus acidophilus JCM 1132, were assessed for adherence to proteins of the mammalian extracellular matrix. L. crispatus JCM 5810 adhered efficiently to immobilized type IV and I collagens, laminin, and, with a lower affinity, to type V collagen and fibronectin. Read More

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Adhesion of Lactobacillus amylovorus to Insoluble and Derivatized Cornstarch Granules.

Appl Environ Microbiol 1991 Apr;57(4):1128-33

Biopolymer Research Unit, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, and A. E. Staley Manufacturing Company, Decatur, Illinois 62525.

Approximately 70% of the cells in a suspension of the amylolytic bacterium Lactobacillus amylovorus bind to cornstarch granules within 30 min at 25 degrees C. More than 60% of the bound bacteria were removed by formaldehyde (2%) or glycine (1 M) at pH 2.0. Read More

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Influence of the growth conditions on the hydrophobicity of Renibacterium salmoninarum evaluated by different methods.

FEMS Microbiol Lett 1989 Jul;51(1):71-7

Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago de Compostela, Spain.

The influence of medium and salinity on the cell surface hydrophobicity of Renibacterium salmoninarum was investigated using three different methods: bacterial adherence to hydrocarbons (BATH), salt agglutination test (SAT), and binding to nitrocellulose filters (NCF). The possible relationship among hydrophobicity, haemagglutination and adherence to cell lines was also evaluated. R. Read More

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The Reed-Sternberg cell/lymphocyte rosette. I. Properties of rosettes formed between Hodgkin's cell lines and allogeneic lymphocytes.

Br J Cancer 1989 Feb;59(2):165-73

University Department of Pathology, Southampton General Hospital, UK.

The properties of rosettes formed between the Hodgkin's cell lines, L428 and L591, and allogeneic peripheral blood mononuclear cell populations have been investigated. Immunocytochemical analysis showed that the majority of adherent cells were T-cells of both the CD4 and CD8 subsets. Only relatively few B-cells and monocytes were seen to adhere. Read More

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February 1989

Chromatographic analysis of the hydrophobic interactions of rabbit IgG immunoglobulins and their papain-digested fragments by bis-(p-chlorophenyl)-acetic acid coupled to aminohexyl sepharose.

Authors:
K Furuya S Urasawa

Mol Immunol 1982 May;19(5):705-17

The present study revealed that the IgG immunoglobulins of normal or non-immune rabbit IgG and anti-bovine serum albumin, anti-ovalbumin, anti-bovine IgG and anti-p-chlorobenzoic acid antibodies could non-specifically bind to bis-(p-chlorophenyl)-acetic acid (DDA) coupled to omega-amino-hexyl Sepharose (AHS) by the use of strengthened hydrophobic interactions dependent on the concentration of NaCl. The main binding site on the adsorbent of DDA-substituted AHS (DDA-AHS) was found to be the DDA ligand. The hydrophobic potency of the DDA ligand was thought to be more effective than that of p-chlorobenzoic acid coupled to AHS. Read More

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Purification of estrogen receptors from MCF-7 human breast cancer cells.

Authors:
M T Chong M Lippman

Cancer Res 1980 Sep;40(9):3172-6

We have partially purified human estrogen receptor from MCF-7 breast cancer cells in order to further characterize the chemical and biological properties of the receptor and to prepare specific receptor antibodies for radioimmunoassay. The estrogen receptor from MCF-7 cell cytosol was purified 1,166-fold (27% recovery) over starting cytosol by combining ammonium sulfate precipitation, affinity chromatography, and Sephadex G-100 gel filtration. The affinity resin consisted of estrone 17-(O-carboxymethyl)oxime:bovine serum albumin: Sepharose 4B. Read More

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September 1980

Synthesis and secretion of colonic glycoproteins: evidence for shedding in vivo of low molecular weight membrane components.

Biochim Biophys Acta 1980 May;629(3):553-65

In vivo glycoprotein synthesis and secretion was studied in rat colonic epithelial cells using precursor labelling with radiolabelled glucosamine. Sepharose 4B gel filtration of radiolabelled glycoproteins obtained from isolated colonic epithelial cells revealed two major fractions: (1) high molecular weight mucus in the excluded fraction and (2) lower molecular weight glycoproteins in the included volume. These glycoproteins were further fractionated by affinity chromatography on concanavalin A-Sepharose. Read More

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Independent effects of IgG and complement upon human polymorphonuclear leukocyte function.

J Immunol 1976 Oct;117(4):1282-7

Particle ingestion by polymorphonuclear leukocytes (PMN) is promoted by cell surface recognition and binding of fragments of the third component of complement (C3) and Fc regions of certain immunoglobulin (IgG) molecules. In order to determine the influence of these specific ligandsurface membrane interactions upon other PMN functions, we have employed nonphagocytosable particles (serum-treated Sepharose beads) coated with fragments of C3 and/or IgG, and have investigated whether these provide a sufficient stimulus for the metabolic changes and degranulation that ordinarly accompany phagocytosis by PMN. Sepharose 4B activates complement in fresh normal serum and consequently is coated with fragments of C3 (confirmed by immunoelectrophoretic evidence of factor B and C3 conversion and by immunofluorescence). Read More

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October 1976
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