4,387 results match your criteria adenovirus-mediated gene

Platelet-rich plasma attenuates interleukin-1β-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2α.

Tissue Cell 2021 Sep 8;73:101646. Epub 2021 Sep 8.

Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, 95 Yong an Road, Xicheng District, Beijing, 100050, China. Electronic address:

Osteoarthritis (OA) is a prevailing chronic disease in Orthopedics that characterized with severely damaged cartilage and subchondral bone, thus leading to profound disorders of synovial joints. Platelet-rich plasma (PRP) has been applied as a popular non-operative treatment option for promoting musculoskeletal healing. Our previous work demonstrated that PRP protected chondrocytes from interleukin-1β (IL-1β)-induced apoptosis in vitro. Read More

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September 2021

Comparison of CRISPR and adenovirus-mediated Myd88 knockdown in RAW 264.7 cells and responses to lipopolysaccharide stimulation.

J Biol Methods 2021 15;8(3):e151. Epub 2021 Jul 15.

Military Operational Medicine Research Program, 810 Schreider St./MRDC-RTO/B201, Ft. Detrick, MD 21702, USA.

Genomic manipulation offers the possibility for novel therapies in lieu of medical interventions in use today. The ability to genetically restore missing inflammatory genes will have a monumental impact on our current immunotherapy treatments. This study compared the efficacy of two different genetic manipulation techniques: clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) transfection to adenoviral transduction to determine which method would provide the most transient and stable knockdown of myeloid differentiation primary response 88 (MyD88). Read More

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Using CRISPR Interference as a Therapeutic Approach to Treat TGFβ2-Induced Ocular Hypertension and Glaucoma.

Invest Ophthalmol Vis Sci 2021 09;62(12)

Eugene & Marilyn Glick Eye Institute, Department of Ophthalmology, Indiana University School of Medicine, Indianapolis, Indiana, United States.

Purpose: Primary open angle glaucoma (POAG) is a leading cause of blindness worldwide with elevated intraocular pressure (IOP) as the most important risk factor. POAG IOP elevation is due to pathological changes in the trabecular meshwork (TM). Elevated TGFβ2 contributes to these changes and increases IOP. Read More

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September 2021

Aluminum particles generated during millisecond electric pulse application enhance adenovirus-mediated gene transfer in L929 cells.

Sci Rep 2021 Sep 6;11(1):17725. Epub 2021 Sep 6.

CNRS, Institut Gustave Roussy, Université Paris-Saclay, Aspects métaboliques et systémiques de l'oncogenèse pour de nouvelles approches thérapeutiques, UMR 9018, 114 rue Edouard Vaillant, F-94805, Villejuif, France.

Gene electrotransfer is an attractive method of non-viral gene delivery. However, the mechanism of DNA penetration across the plasma membrane is widely discussed. To explore this process for even larger structures, like viruses, we applied various combinations of short/long and high/low-amplitude electric pulses to L929 cells, mixed with a human adenovirus vector expressing GFP. Read More

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September 2021

Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes.

mSystems 2021 Aug 31:e0046821. Epub 2021 Aug 31.

Division of Protective Immunity and Division of Cancer Pathobiology, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.

Viral infections are associated with extensive remodeling of the cellular proteome. Viruses encode gene products that manipulate host proteins to redirect cellular processes or subvert antiviral immune responses. Adenovirus (AdV) encodes proteins from the early E4 region which are necessary for productive infection. Read More

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Long Noncoding RNA Inhibits Lung Fibroblast Proliferation via a Reduction of β-Catenin.

Int J Mol Sci 2021 Aug 9;22(16). Epub 2021 Aug 9.

Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, OK 74078, USA.

Idiopathic Pulmonary Fibrosis (IPF) is a chronic, progressive, and usually lethal lung disease and it has been widely accepted that fibroblast proliferation is one of the key characteristics of IPF. Long noncoding RNAs (lncRNAs) play vital roles in the pathogenesis of many diseases. In this study, we investigated the role of lncRNA on fibroblast proliferation. Read More

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Adenoviral Transduction of Dickkopf-1 Alleviates Silica-induced Silicosis Development in Lungs of Mice.

Hum Gene Ther 2021 Aug 18. Epub 2021 Aug 18.

The University of Iowa, 4083, The Center for Gene Therapy of Cystic Fibrosis and Other Genetic Diseases, Iowa City, Iowa, United States.

Silicosis is an occupational disease caused by inhalation of silica dust, which is hallmarked by progressive pulmonary fibrosis associated with poor prognosis. Wnt/β-catenin signaling is implicated in the development of fibrosis and is a therapeutic target for fibrotic diseases. Previous clinical studies of patients with pneumoconioses including silicosis revealed an increased concentration of circulating WNT3A and DKK1 proteins and inflammatory cells in bronchoalveolar lavage compared to healthy subjects. Read More

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Liver-Specific Deletion of Mouse CTCF Leads to Hepatic Steatosis via Augmented PPARγ Signaling.

Cell Mol Gastroenterol Hepatol 2021 Aug 4. Epub 2021 Aug 4.

Department of Environmental Medical Biology, Institute of Tropical Medicine, Seoul, Korea; Brain Korea 21 PLUS Project for Medical Science, Seoul, Korea. Electronic address:

Background & Aims: The liver is the major organ for metabolizing lipids, and malfunction of the liver leads to various diseases. Nonalcoholic fatty liver disease is rapidly becoming a major health concern worldwide and is characterized by abnormal retention of excess lipids in the liver. CCCTC-binding factor (CTCF) is a highly conserved zinc finger protein that regulates higher-order chromatin organization and is involved in various gene regulation processes. Read More

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Combined therapy of adenovirus vector mediated IGF-1 gene with anti-CD20 mAbs exerts potential beneficial role on type 1 diabetes in nonobese diabetic mice.

Life Sci 2021 Jul 28:119853. Epub 2021 Jul 28.

Department of Medical College, Shenzhen Polytechnic, Shenzhen 518055, Guangdong Province, PR China. Electronic address:

Aims: To assess the protective effects of combined treatment with anti-CD20 monoclonal antibody (mAb) and adenovirus mediated mouse insulin-like growth factor 1 (Adv-mIGF-1) gene on type 1 diabetes (T1D) in nonobese diabetic (NOD) mice at early stage.

Methods: To simultaneously restore the proportion of Th cells and block the interaction of B cells, NOD model mice were assigned to four groups which received PBS, Adv-mIGF-1 gene and anti-CD20 mAbs alone or combination, respectively. After 16 weeks of therapeutic intervention, blood samples and pancreatic tissues of mice were measured via the methods of ELISA, RT-PCR, western blotting, H&E staining, TUNEL and immunohistochemistry assays. Read More

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SDF4 Is a Prognostic Factor for 28-Days Mortality in Patients With Sepsis Negatively Regulating ER Stress.

Front Immunol 2021 13;12:659193. Epub 2021 Jul 13.

Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Sepsis is a heterogeneous syndrome induced by infection and results in high mortality. Even though more than 100 biomarkers for sepsis prognosis were evaluated, prediction of patient outcomes in sepsis continues to be driven by clinical signs because of unsatisfactory specificity and sensitivity of these biomarkers. This study aimed to elucidate the key candidate genes involved in sepsis response and explore their downstream effects based on weighted gene co-expression network analysis (WGCNA). Read More

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Insulin-like growth factor 1 gene transfer for sporadic Alzheimer's disease: New evidence for trophic factor mediated hippocampal neuronal and synaptic recovery-based behavior improvement.

Hippocampus 2021 Oct 29;31(10):1137-1153. Epub 2021 Jul 29.

Institute for Biochemical Research (INIBIOLP)-National Scientific and Technical Research Council (CONICET)-School of Medical Sciences, National University of La Plata (UNLP), La Plata, Argentina.

Sporadic Alzheimer's disease (sAD) is the most prevalent neurodegenerative disorder with no cure. Patients typically suffer from cognitive impairment imprinted by irreversible neocortex and hippocampal degeneration with overt synaptic and neuron dysfunction. Insulin-like growth factor 1 (IGF1) has proven to be a potent neuroprotective molecule in animal models of age-related neurodegeneration. Read More

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October 2021

Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system.

Sci Rep 2021 Jul 20;11(1):14746. Epub 2021 Jul 20.

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Korea.

Zebra finch is a representative animal model for studying the molecular basis of human disorders of vocal development and communication. Accordingly, various functional studies of zebra finch have knocked down or introduced foreign genes in vivo; however, their germline transmission efficiency is remarkably low. The primordial germ cell (PGC)-mediated method is preferred for avian transgenic studies; however, use of this method is restricted in zebra finch due to the lack of an efficient gene transfer method for the germline. Read More

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Branched-chain amino acid metabolism is regulated by ERRα in primary human myotubes and is further impaired by glucose loading in type 2 diabetes.

Diabetologia 2021 Sep 16;64(9):2077-2091. Epub 2021 Jun 16.

Department of Molecular Medicine and Surgery, Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.

Aims/hypothesis: Increased levels of branched-chain amino acids (BCAAs) are associated with type 2 diabetes pathogenesis. However, most metabolomic studies are limited to an analysis of plasma metabolites under fasting conditions, rather than the dynamic shift in response to a metabolic challenge. Moreover, metabolomic profiles of peripheral tissues involved in glucose homeostasis are scarce and the transcriptomic regulation of genes involved in BCAA catabolism is partially unknown. Read More

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September 2021

EZH2-mediated inhibition of KLF14 expression promotes HSCs activation and liver fibrosis by downregulating PPARγ.

Cell Prolif 2021 Jul 24;54(7):e13072. Epub 2021 May 24.

Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Department of Gastroenterology, Institute of Liver and Gastrointestinal Diseases, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objectives: Induction of deactivation and apoptosis of hepatic stellate cells (HSCs) are principal therapeutic strategies for liver fibrosis. Krüppel-like factor 14 (KLF14) regulates various biological processes, however, roles, mechanisms and implications of KLF14 in liver fibrosis are unknown.

Materials And Methods: KLF14 expression was detected in human, rat and mouse fibrotic models, and its effects on HSCs were assessed. Read More

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Indoleamine 2,3-Dioxygenase 1 (IDO1) Promotes Cardiac Hypertrophy via a PI3K-AKT-mTOR-Dependent Mechanism.

Cardiovasc Toxicol 2021 Aug 21;21(8):655-668. Epub 2021 May 21.

Department of Anesthesiology, First Affiliated Hospital of Jiamusi University, Jiamusi, China.

Indoleamine 2,3-dioxygenase 1 (IDO1) is an enzyme for tryptophan metabolism, involved in immune cell differentiation/maturation and cancer biology. IDO1 is also expressed in cardiomyocytes, but its roles in the cardiovascular system are not fully understood. Here, we reported the functions of IDO1 during cardiac hypertrophy. Read More

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Adenovirus-Mediated Inducible Expression of a PD-L1 Blocking Antibody in Combination with Macrophage Depletion Improves Survival in a Mouse Model of Peritoneal Carcinomatosis.

Int J Mol Sci 2021 Apr 17;22(8). Epub 2021 Apr 17.

Program of Gene Therapy and Regulation of Gene Expression, Cima Universidad of Navarra, 31008 Pamplona, Spain.

Immune checkpoint inhibitors (ICIs) have demonstrated remarkable efficacy in a growing number of malignancies. However, overcoming primary or secondary resistances is difficult due to pharmacokinetics issues and side effects associated with high systemic exposure. Local or regional expression of monoclonal antibodies (mAbs) using gene therapy vectors can alleviate this problem. Read More

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TRAIL induces proliferation in rodent pancreatic beta cells via AKT activation.

J Mol Endocrinol 2021 05 11;66(4):325-338. Epub 2021 May 11.

Center for Gene and Cell Therapy, Akdeniz University, Antalya, Turkey.

Strategies to increase functional pancreatic beta cell mass is of great interest in diabetes-related research. TNF-related apoptosis-inducing ligand (TRAIL) is well known to promote proliferation and survival in various cell types, including vascular smooth muscle and endothelial cells. Correlation between the protective nature of TRAIL on these cells and its proliferative effect is noteworthy. Read More

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Cytokine-induced killer cells carrying recombinant oncolytic adenovirus expressing p21Ras scFv inhibited liver cancer.

J Cancer 2021 10;12(9):2768-2776. Epub 2021 Mar 10.

Graduate School, Kunming Medical University, Kunming, Yunnan, China.

Oncolytic adenovirus-mediated gene therapy is an emerging strategy for cancer treatment. However, oncolytic adenoviruses are mainly administered locally at tumor site. Intravenous administration of oncolytic adenovirus for cancer gene therapy is a problem that needs to be solved urgently. Read More

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Upregulated endonuclease Regnase-1 suppresses osteoarthritis by forming a negative feedback loop of catabolic signaling in chondrocytes.

Arthritis Res Ther 2021 04 14;23(1):114. Epub 2021 Apr 14.

National Creative Research Initiatives Center for Osteoarthritis Pathogenesis and School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 61005, Republic of Korea.

Background: Ribonucleases (RNases) play central roles in the post-transcriptional regulation of mRNA stability. Our preliminary results revealed that the endonuclease Regnase-1 is specifically upregulated in osteoarthritic chondrocytes. We herein explored the possible functions and regulatory mechanisms of Regnase-1 in a mouse model of osteoarthritis (OA). Read More

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- can be regulated by via the NF-κB pathway in hypoxia-induced liver fibrosis.

Ann Transl Med 2021 Mar;9(6):505

Department of Interventional Radiology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.

Background: The identification of the important elements that control hepatic stellate cell (HSC) activation will expand our understanding of the mechanism of liver fibrosis induced by hypoxia and affect the outcome of clinical treatment. A previous research demonstrated that N-Myc downstream-regulated gene 2 () is a potential regulator of fibrosis and a downstream target gene of hypoxia-inducible factor 1 (). In this research, we studied the expression and function of NDRG2 in liver fibrosis induced by hypoxia. Read More

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[Regulation of bFGF and TGF-β2 in human scleral fibroblasts by the lumican gene mutation associated with myopia].

Zhonghua Yan Ke Za Zhi 2021 Apr;57(4):277-283

Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University, Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing 100730, China.

To investigate the regulation of basic fibroblast growth factor (bFGF) and transforming growth factor (TGF)-β2 in human scleral fibroblasts (HSFs) by the adenovirus-mediated Lumican gene mutation, and to illustrate the effect of this mutation on myopia. Experimental study. The HSFs were isolated and cultured from human scleral tissues. Read More

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KLF5-mediated Eppk1 expression promotes cell proliferation in cervical cancer via the p38 signaling pathway.

BMC Cancer 2021 Apr 8;21(1):377. Epub 2021 Apr 8.

Department of Oncology, the Second Hospital of Tangshan, Tangshan, 063000, China.

Background: Epiplakin1 (Eppk1) is part of epidermal growth factor (EGF) signal and takes part in reorganization of cytoskeleton and cell proliferation. However, the role of Eppk1 in cervical cancer (CC) remains unknown.

Methods: To express Eppk1 and KLF5 and their correlation, we used RNA-sequence, RT-qPCR, TCGA database and immunofluorescence staining in vitro and in different pathological cervical tissues. Read More

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PRMT4 drives post-ischemic angiogenesis via YB1/VEGF signaling.

J Mol Med (Berl) 2021 Jul 6;99(7):993-1008. Epub 2021 Apr 6.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430000, China.

Angiogenesis is an integral process in many ischemic disorders, and vascular endothelial growth factor (VEGF) plays an important role in it. Protein arginine methyltransferase 4 (PRMT4), a member of the type I PRMT family, is involved in various biological activities, but its role in endothelial cell (EC) remains elusive. Here, we aimed to investigate the role of PRMT4 in ischemic angiogenesis and explore the possible underlying mechanism. Read More

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Systemic administration of mesenchymal stem cells loaded with a novel oncolytic adenovirus carrying IL-24/endostatin enhances glioma therapy.

Cancer Lett 2021 Jul 2;509:26-38. Epub 2021 Apr 2.

Laboratory of Gene Therapy, Department of Biochemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, PR China. Electronic address:

Oncolytic adenovirus-mediated gene therapy shows promise for cancer treatment; however, the systemic delivery of oncolytic adenovirus to tumors remains challenging. Recently, mesenchymal stem cells (MSCs) have emerged as potential vehicles for improving delivery. Yet, because the oncolytic adenovirus replicates in MSCs, balancing MSC viability with viral load is key to achieving optimal therapeutic effect. Read More

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Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss.

Front Cell Dev Biol 2021 12;9:640867. Epub 2021 Mar 12.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Osteoporosis is a common chronic metabolic bone disease characterized by reduced trabecular bone and increased bone fragility. Monoacylglycerol lipase (MAGL) is a lipolytic enzyme to catalyze the hydrolysis of monoglycerides and specifically degrades the 2-arachidonoyl glycerol (2-AG). Previous studies have identified that 2-AG is the mainly source for arachidonic acid and the most abundant endogenous agonist of cannabinoid receptors. Read More

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FOXA3, a Negative Regulator of Nur77 Expression and Activity in Testicular Steroidogenesis.

Int J Endocrinol 2021 3;2021:6619447. Epub 2021 Mar 3.

School of Biological Sciences and Technology, Chonnam National University, Gwangju, Republic of Korea.

Biosynthesis of testosterone occurs mainly in the testicular Leydig cells. Nur77, an orphan nuclear receptor that is expressed in response to the luteinizing hormone/cyclic adenosine monophosphate (LH/cAMP) signaling pathway, is one of the key factors that regulate steroidogenesis in Leydig cells. The function of Nur77 is modulated through interaction with other proteins. Read More

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A novel role of kallikrein-related peptidase 8 in the pathogenesis of diabetic cardiac fibrosis.

Theranostics 2021 20;11(9):4207-4231. Epub 2021 Feb 20.

Department of Physiology, Navy Medical University, Shanghai, China.

Among all the diabetic complications, diabetic cardiomyopathy, which is characterized by myocyte loss and myocardial fibrosis, is the leading cause of mortality and morbidity in diabetic patients. Tissue kallikrein-related peptidases (KLKs) are secreted serine proteases, that have distinct and overlapping roles in the pathogenesis of cardiovascular diseases. However, whether KLKs are involved in the development of diabetic cardiomyopathy remains unknown. Read More

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SIRT2 ablation inhibits glucose-stimulated insulin secretion through decreasing glycolytic flux.

Theranostics 2021 4;11(10):4825-4838. Epub 2021 Mar 4.

Department of Endocrine and Metabolic Diseases/ Shanghai institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Sirtuins are NAD-dependent protein deacylases known to have protective effects against age-related diseases such as diabetes, cancer, and neurodegenerative disease. SIRT2 is the only primarily cytoplasmic isoform and its overall role in glucose homeostasis remains uncertain. SIRT2-knockout (KO) rats were constructed to evaluate the role of SIRT2 in glucose homeostasis. Read More

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Hypoxia protects H9c2 cells against Ferroptosis through SENP1-mediated protein DeSUMOylation.

Int J Med Sci 2021 4;18(7):1618-1627. Epub 2021 Feb 4.

Beijing Institute of Radiation Medicine, Beijing, 100850, PR China.

Hypoxia affects proliferation, differentiation, as well as death of cardiomyocyte, and plays an important role in the development of myocardial ischemia. However, the detailed mechanisms through which hypoxia regulates cardiomyocyte ferroptosis have not been explored. In this study, we revealed that hypoxia suppresses the proliferation, migration, and erastin-induced ferroptosis of H9c2 cells. Read More

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February 2021

Aerobic glycolysis supports hepatitis B virus protein synthesis through interaction between viral surface antigen and pyruvate kinase isoform M2.

PLoS Pathog 2021 03 15;17(3):e1008866. Epub 2021 Mar 15.

Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan.

As an intracellular pathogen, the reproduction of the hepatitis B virus (HBV) depends on the occupancy of host metabolism machinery. Here we test a hypothesis if HBV may govern intracellular biosynthesis to achieve a productive reproduction. To test this hypothesis, we set up an affinity purification screen for host factors that interact with large viral surface antigens (LHBS). Read More

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