J Virol Methods 2020 07 12;281:113880. Epub 2020 May 12.
Department of Microbiology, Immunology, and Parasitology, USA; Department of Ophthalmology, USA; The Stanley S. Scott Cancer Center, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA, 70112, USA; The Louisiana Vaccine Center, New Orleans, LA, 70112, USA. Electronic address:
The adenoviral genome encodes coordinately expressed early and late gene transcriptional units that specify a complex collection of extensively spliced overlapping mRNAs. These complexities confound the generation of compatible, validated and optimized qPCR assays that permit comprehensive evaluation of adenoviral transcription. We have developed and evaluated a compilation of qPCR assays that represent the majority of the human adenovirus 5 (hAdV5) genome and allow for absolute and relative quantification of transcriptional activity. Read More