4 results match your criteria adapters trimmed

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Comparative genome characterization of Leptospira interrogans from mild and severe leptospirosis patients.

Genomics Inform 2021 Sep 30;19(3):e31. Epub 2021 Sep 30.

Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Leptospirosis is a zoonotic disease caused by spirochetes from the genus Leptospira. In Thailand, Leptospira interrogans is a major cause of leptospirosis. Leptospirosis patients present with a wide range of clinical manifestations from asymptomatic, mild infections to severe illness involving organ failure. Read More

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September 2021

First report of Grapevine yellow speckle viroid 1 infecting grapevine (Vitis vinifera L.) in Canada.

Plant Dis 2021 Jun 7. Epub 2021 Jun 7.

Agriculture and AgriFood Canada , Research and development center , 430 Gouin, Saint-Jean-sur-Richelieu, Quebec, Canada, J3B3E6;

Quebec is the third largest wine grape producer in Canada in acreage, tonnage, and wine grape sales (Carisse et al. 2017; Ben Moussa et al. 2019). Read More

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Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo.

Methods Mol Biol 2017 ;1562:269-281

Centre for Biochemistry and Molecular Cell Biology, Institute for Molecular Biology, Georg-August-University, Humboldtallee 23, 37073, Göttingen, Germany.

Several crosslinking methods have been developed to identify interacting RNAs for proteins of interest. Here, we describe variants of the UV crosslinking and analysis of cDNA (CRAC) method that allow target identification of RNA methyltransferases on a genome-wide scale. We present a detailed protocol for the application of CRAC in human cells that stably express the protein of interest fused to a tandem affinity tag. Read More

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February 2018

Analysis of RNA-Seq data with TopHat and Cufflinks for genome-wide expression analysis of jasmonate-treated plants and plant cultures.

Methods Mol Biol 2013 ;1011:305-15

Department of Plant Systems Biology, VIB, Gent, Belgium.

The recent development of various deep sequencing techniques has led to the most powerful transcript profiling method available to date, RNA sequencing or RNA-Seq. Besides the identification of new genes and new splice variants of known genes, RNA-Seq allows to compare the whole transcriptome of any organism under two or more experimental conditions, such as before and after jasmonate treatment. However, the vast amounts of data generated during RNA-Seq experiments require complex computational methods for read mapping and expression quantification. Read More

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October 2013
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