Methods Mol Biol 2017 ;1562:269-281
Centre for Biochemistry and Molecular Cell Biology, Institute for Molecular Biology, Georg-August-University, Humboldtallee 23, 37073, Göttingen, Germany.
Several crosslinking methods have been developed to identify interacting RNAs for proteins of interest. Here, we describe variants of the UV crosslinking and analysis of cDNA (CRAC) method that allow target identification of RNA methyltransferases on a genome-wide scale. We present a detailed protocol for the application of CRAC in human cells that stably express the protein of interest fused to a tandem affinity tag. Read More