51,804 results match your criteria activity yeasts

Inhibition of polymicrobial biofilm formation by saw palmetto oil, lauric acid and myristic acid.

Microb Biotechnol 2021 Jun 22. Epub 2021 Jun 22.

School of Chemical Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Korea.

Biofilms are communities of bacteria, fungi or yeasts that form on diverse biotic or abiotic surfaces, and play important roles in pathogenesis and drug resistance. A generic saw palmetto oil inhibited biofilm formation by Staphylococcus aureus, Escherichia coli O157:H7 and fungal Candida albicans without affecting their planktonic cell growth. Two main components of the oil, lauric acid and myristic acid, are responsible for this antibiofilm activity. Read More

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and antifungal activity of buparvaquone against .

Antimicrob Agents Chemother 2021 Jun 21:AAC0069921. Epub 2021 Jun 21.

Laboratory of Fungal Cell Biology, Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

Sporotrichosis has become an important zoonosis in Brazil and is the primary species transmitted by cats. Improvement of animal treatment will help control and limit the spread and geographic expansion of sporotrichosis. Accordingly, buparvaquone, an antiprotozoal hydroxynaphthoquinone agent marketed as Butalex®, was evaluated and against feline-borne isolates of . Read More

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Hsp104 contributes to freeze-thaw tolerance by maintaining proteasomal activity in a spore clone isolated from Shirakami kodama yeast.

J Gen Appl Microbiol 2021 Jun 19. Epub 2021 Jun 19.

Akita Research Institute of Food and Brewing.

The supply of oven-fresh bakery products to consumers has been improved by frozen dough technology; however, freeze-thaw stress decreases the activity of yeast cells. To breed better baker's yeasts for frozen dough, it is important to understand the factors affecting freeze-thaw stress tolerance in baker's yeast. We analyzed the stress response in IB1411, a spore clone from Saccharomyces cerevisiae Shirakami kodama yeast, with an exceptionally high tolerance to freeze-thaw stress. Read More

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Yeasts isolated from a lotic continental environment in Brazil show potential to produce amylase, cellulase and protease.

Biotechnol Rep (Amst) 2021 Jun 29;30:e00630. Epub 2021 May 29.

Universidade Estadual do Oeste do Paraná, Brazil.

Yeasts have wide applicability in the industrial field, as in the production of enzymes used in biocatalysts. Biocatalysts are more efficient when compared to chemical catalysts, with emphasis on hydrolytic enzymes, such as amylase, cellulase and protease. Here we focused on prospecting yeasts, with a high capacity to synthesize hydrolytic enzymes, from a continental lotic ecosystem environment in Brazil. Read More

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Molecular identification, virulence factors and antifungal susceptibility patterns of complex species isolated from clinical samples.

Niger J Clin Pract 2021 Jun;24(6):853-859

Department of Medical Microbiology, School of Medicine, Erciyes University, Kayseri, Turkey.

Aims: The aim of this study was to identify C. parapsilosis complex strains isolated from various clinical samples by sequence analysis and to investigate whether there are any differences between the species in terms of virulence factors and antifungal susceptibility.

Methods: The study included a total of 42 isolates identified as C. Read More

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Method of salivary histatins determination.

Klin Lab Diagn 2021 Jun;66(6):358-363

G.N. Gabrichevsky research institute for epidemiologyµbiology.

Histatins are the most significant antimicrobial peptides (AMP) of saliva and there are 12 types of such AMP. Histatin molecules contain relatively high percent of histidine and tyrosine residues. This property allows to use well known from organic chemistry Pauly reaction for detection of protein bounded histidine and tyrosine residues (BHT), which are in fact characterize the summary content of all histatins in saliva. Read More

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A bamboo leaf-specific aquaporin gene PePIP2;7 is involved in abiotic stress response.

Plant Cell Rep 2021 Jun 7. Epub 2021 Jun 7.

Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and Technology, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan, Beijing, 100102, China.

Key Message: PePIP2;7, a leaf-specific aquaporin gene in bamboo, is upregulated under abiotic stresses. Overexpressing PePIP2;7 confers abiotic stresses tolerance in transgenic Arabidopsis plant and yeast. Aquaporins (AQPs) participate in the regulation of water balance in plants. Read More

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Safety Evaluation of Yeasts With Probiotic Potential.

Front Nutr 2021 21;8:659328. Epub 2021 May 21.

Analytical Chemistry and Food Technology Department and Instituto Regional de Investigación Científica Aplicada (IRICA), Faculty of Chemical Sciences and Technologies, Castilla-La Mancha University, Ciudad Real, Spain.

This work has evaluated the safety aspects of 20 yeast strains, isolated from food environments, selected in previous works due to their probiotic potential. Among the different strains, there are and non- yeasts. Before safety evaluation, differentiation of strains was done by PCR amplification of inter-δ region with pairs of primers δ2-12 and δ12-21, which showed that they were all different from each other and also had different profiles to (the only commercial probiotic yeast). Read More

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Comparative uptake of exogenous thiamine and subsequent metabolic footprint in Saccharomyces cerevisiae and Kluyveromyces marxianus under simulated oenological conditions.

Int J Food Microbiol 2021 Apr 21:109206. Epub 2021 Apr 21.

South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Private Bag X1, Matieland 7602, South Africa. Electronic address:

Managed inoculation of non-Saccharomyces yeast species is regarded as a practical way to introduce new characteristics to wine. However, these yeasts struggle to survive until fermentation is complete. Kluyveromyces marxianus IWBT Y885 is one such yeast. Read More

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Assaying the Molecular Determinants and Kinetics of RNA Pseudouridylation by H/ACA snoRNPs and Stand-Alone Pseudouridine Synthases.

Methods Mol Biol 2021 ;2298:357-378

Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, Lethbridge, AB, Canada.

Posttranscriptional modifications of RNA play an important role in promoting the maturation and functional diversity of many RNA species. Accordingly, understanding the enzymes and mechanisms that underlie RNA modifications is an important aspect in advancing our knowledge of the continually expanding RNA modification field. However, of the more than 160 currently identified RNA modifications, a large portion remains without quantitative detection assays for their biochemical characterization. Read More

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Enhancement of astaxanthin accumulation using black light in Coelastrum and Monoraphidium isolated from Malaysia.

Sci Rep 2021 Jun 3;11(1):11708. Epub 2021 Jun 3.

Euglena Co., Ltd, Tokyo, 108-0014, Japan.

Microalgae are important microorganisms which produce potentially valuable compounds. Astaxanthin, a group of xanthophyll carotenoids, is one of the most powerful antioxidants mainly found in microalgae, yeasts, and crustaceans. Environmental stresses such as intense light, drought, high salinity, nutrient depletion, and high temperature can induce the accumulation of astaxanthin. Read More

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Investigational Agents for the Treatment of Resistant Yeasts and Molds.

Curr Fungal Infect Rep 2021 May 28:1-12. Epub 2021 May 28.

Department of Internal Medicine, Division of Infectious Diseases, University of Texas Health Science Center at Houston, McGovern Medical School, Houston, TX USA.

Purpose Of Review: This review summarizes the investigational antifungals in clinical development with the potential to address rising drug resistance patterns. The relevant pharmacodynamics, spectrum of activity, preclinical studies, and latest clinical trial data are described.

Recent Findings: Agricultural and medicinal antifungal use has been selected for inherently drug-resistant fungi and acquired resistance mechanisms. Read More

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Repositioning Lopinavir, an HIV Protease Inhibitor, as a Promising Antifungal Drug: Lessons Learned from -In Silico, In Vitro and In Vivo Approaches.

J Fungi (Basel) 2021 May 28;7(6). Epub 2021 May 28.

Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes (LEAMER), Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-901, Brazil.

The repurposing strategy was applied herein to evaluate the effects of lopinavir, an aspartic protease inhibitor currently used in the treatment of HIV-infected individuals, on the globally widespread opportunistic human fungal pathogen by using in silico, in vitro and in vivo approaches in order to decipher its targets on fungal cells and its antifungal mechanisms of action. Secreted aspartic proteases (Saps) are the obviously main target of lopinavir. To confirm this hypothesis, molecular docking assays revealed that lopinavir bound to the Sap2 catalytic site of as well as inhibited the Sap hydrolytic activity in a typically dose-dependent manner. Read More

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Bioreactor Co-Cultivation of High Lipid and Carotenoid Producing Yeast and Several Microalgae under Stress.

Microorganisms 2021 May 28;9(6). Epub 2021 May 28.

Faculty of Chemistry, Brno University of Technology, 612 00 Brno, Czech Republic.

The co-cultivation of red yeasts and microalgae works with the idea of the natural transport of gases. The microalgae produce oxygen, which stimulates yeast growth, while CO produced by yeast is beneficial for algae growth. Both microorganisms can then produce lipids. Read More

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Structure-Activity Relationship Assessment of Sophorolipid Ester Derivatives against Model Bacteria Strains.

Molecules 2021 May 19;26(10). Epub 2021 May 19.

Center for Biotechnology and Interdisciplinary Studies (CBIS), Rensselaer Polytechnic Institute, Troy, NY 12180, USA.

Sophorolipids (SLs) are glycolipids that consist of a hydrophilic sophorose head group covalently linked to a hydrophobic fatty acid tail. They are produced by fermentation of non-pathogenic yeasts such as . The fermentation products predominantly consist of the diacetylated lactonic form that coexists with the open-chain acidic form. Read More

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Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains.

Biomolecules 2021 05 17;11(5). Epub 2021 May 17.

Center for Protein Studies, Faculty of Biology, University of Havana, 25 St, La Habana 10400, Cuba.

Cm-p5 is a snail-derived antimicrobial peptide, which demonstrated antifungal activity against the pathogenic strains of . Previously we synthetized a cyclic monomer as well as a parallel and an antiparallel dimer of Cm-p5 with improved antifungal activity. Considering the alarming increase of microbial resistance to conventional antibiotics, here we evaluated the antimicrobial activity of these derivatives against multiresistant and problematic bacteria and against important viral agents. Read More

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The Meiosis-Specific Crs1 Cyclin Is Required for Efficient S-Phase Progression and Stable Nuclear Architecture.

Int J Mol Sci 2021 May 22;22(11). Epub 2021 May 22.

Instituto de Biología Funcional y Genómica (IBFG), Consejo Superior de Investigaciones Científicas, Universidad de Salamanca, 37007 Salamanca, Spain.

Cyclins and CDKs (Cyclin Dependent Kinases) are key players in the biology of eukaryotic cells, representing hubs for the orchestration of physiological conditions with cell cycle progression. Furthermore, as in the case of meiosis, cyclins and CDKs have acquired novel functions unrelated to this primal role in driving the division cycle. Meiosis is a specialized developmental program that ensures proper propagation of the genetic information to the next generation by the production of gametes with accurate chromosome content, and meiosis-specific cyclins are widespread in evolution. Read More

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Exposure to the Methylselenol Precursor Dimethyldiselenide Induces a Reductive Endoplasmic Reticulum Stress in .

Int J Mol Sci 2021 May 22;22(11). Epub 2021 May 22.

Laboratoire de Biologie Structurale de la Cellule, BIOC, École Polytechnique, CNRS-UMR7654, IP Paris, 91128 Palaiseau, France.

Methylselenol (MeSeH) is a major cytotoxic metabolite of selenium, causing apoptosis in cancer cells through mechanisms that remain to be fully established. Previously, we demonstrated that, in , MeSeH toxicity was mediated by its metabolization into selenomethionine by O-acetylhomoserine (OAH)-sulfhydrylase, an enzyme that is absent in higher eukaryotes. In this report, we used a mutant yeast strain, devoid of OAH- sulfhydrylase activity, to identify alternative targets of MeSeH. Read More

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Antimicrobial Activities of LL-37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate against , , and .

Int J Mol Sci 2021 May 12;22(10). Epub 2021 May 12.

Faculty of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien 9-Bancho, Nishinomiya City 663-8179, Japan.

Various peptides and their derivatives have been reported to exhibit antimicrobial activities. Although these activities have been examined against microorganisms, novel methods have recently emerged for conjugation of the biomaterials to improve their activities. Here, we prepared CKR12-PLGA, in which CKR12 (a mutated fragment of human cathelicidin peptide, LL-37) was conjugated with poly (lactic-co-glycolic) acid (PLGA), and compared the antimicrobial and antifungal activities of the conjugated peptide with those of FK13 (a small fragment of LL-37) and CKR12 alone. Read More

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An Eco-Friendly Approach to the Control of Pathogenic Microbes and Malarial Vector Using Magnesium Oxide Nanoparticles (Mg-NPs) Fabricated by .

Int J Mol Sci 2021 May 12;22(10). Epub 2021 May 12.

Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.

The discovery of eco-friendly, rapid, and cost-effective compounds to control diseases caused by microbes and insects are the main challenges. Herein, the magnesium oxide nanoparticles (MgO-NPs) are successfully fabricated by harnessing the metabolites secreted by . The fabricated MgO-NPs were characterized using UV-Vis, XRD, TEM, DLS, EDX, FT-IR, and XPS analyses. Read More

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Drug Repurposing in Medical Mycology: Identification of Compounds as Potential Antifungals to Overcome the Emergence of Multidrug-Resistant Fungi.

Pharmaceuticals (Basel) 2021 May 20;14(5). Epub 2021 May 20.

Aix Marseille Univ, IRD, APHM, MEPHI, 13005 Marseille, France.

Immunodepression, whether due to HIV infection or organ transplantation, has increased human vulnerability to fungal infections. These conditions have created an optimal environment for the emergence of opportunistic infections, which is concomitant to the increase in antifungal resistance. The use of conventional antifungal drugs as azoles and polyenes can lead to clinical failure, particularly in immunocompromised individuals. Read More

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Antimicrobial Polyamide-Alginate Casing Incorporated with Nisin and ε-Polylysine Nanoparticles Combined with Plant Extract for Inactivation of Selected Bacteria in Nitrite-Free Frankfurter-Type Sausage.

Foods 2021 May 4;10(5). Epub 2021 May 4.

Centro Tecnológico de la Carne de Galicia, Parque Tecnológico de Galicia, Avda. Galicia n 4, San Cibrao das Viñas, 32900 Ourense, Spain.

The effects of combining a polyamide-alginate casing incorporated with nisin (100 ppm and 200 ppm) and ε-polylysine (500 ppm and 1000 ppm) nanoparticles and a mixed plant extract as ingredient in sausage formulation (500 ppm; composed of olive leaves (OLE), green tea (GTE) and stinging nettle extracts (SNE) in equal rates) were studied to improve the shelf life and safety of frankfurter-type sausage. The film characteristics and microbiological properties of sausage samples were evaluated. Sausage samples were packaged in polyethylene bags (vacuum condition) and analysed during 45 days of storage at 4 °C. Read More

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Attempts for Developing Novel Sugar-Based and Sugar-Free Sea Buckthorn Marmalades.

Molecules 2021 May 21;26(11). Epub 2021 May 21.

Faculty of Food Science and Engineering, Dunărea de Jos University of Galati, 800008 Galati, Romania.

Sea buckthorn ( L.) is recognized as a valuable source of vitamin C and antioxidants, frequently used as nutraceuticals and cosmeceuticals. In the present study, attempts are made to produce and characterize a novel type of marmalade using sea buckthorn berries processed at 102 °C into marmalade in two combinations, with whole cane or stevia sugar. Read More

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Impact of glycerol feeding profiles on STEAP1 biosynthesis by Komagataella pastoris using a methanol-inducible promoter.

Appl Microbiol Biotechnol 2021 Jun 1;105(11):4635-4648. Epub 2021 Jun 1.

CICS-UBI - Health Sciences Research Centre, University of Beira Interior, 6201-506, Covilhã, Portugal.

Currently, the lack of reliable strategies for the diagnosis and treatment of cancer makes the identification and characterization of new therapeutic targets a pressing matter. Several studies have proposed the Six Transmembrane Epithelial Antigen of the Prostate 1 (STEAP1) as a promising therapeutic target for prostate cancer. Although structural and functional studies may provide deeper insights on the role of STEAP1 in cancer, such techniques require high amounts of purified protein through biotechnological processes. Read More

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Differential interactions of the antimicrobial peptide, RQ18, with phospholipids and cholesterol modulate its selectivity for microorganism membranes.

Biochim Biophys Acta Gen Subj 2021 May 27;1865(9):129937. Epub 2021 May 27.

Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil. Electronic address:

Background: Antimicrobial peptides (AMPs) are molecules with potential application for the treatment of microorganism infections. We, herein, describe the structure, activity, and mechanism of action of RQ18, an α-helical AMP that displays antimicrobial activity against Gram-positive and Gram-negative bacteria, and yeasts from the Candida genus.

Methods: A physicochemical-guided design assisted by computer tools was used to obtain our lead peptide candidate, named RQ18. Read More

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Antimicrobial Activity of First-in-Class Antifungal, Manogepix, and Comparator Agents Testing against Contemporary Invasive Fungal Isolates from an International Surveillance Program (2018-2019): In Vitro Activity of Manogepix against Fungal Isolates.

J Glob Antimicrob Resist 2021 May 26. Epub 2021 May 26.

JMI Laboratories, North Liberty, IA, USA.

Objectives: Manogepix, the active moiety of the prodrug fosmanogepix, is a novel antifungal with activity against major fungal pathogens including Candida (except C. krusei), Aspergillus, and difficult-to-treat/rare moulds. We tested manogepix and comparators against 2,669 contemporary (2018-2019) fungal isolates collected from 82 medical centers located in North America (42. Read More

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Marine microbial L-glutaminase: from pharmaceutical to food industry.

Appl Microbiol Biotechnol 2021 Jun 27;105(11):4453-4466. Epub 2021 May 27.

Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.

Deamination of L-glutamine to glutamic acid with the concomitant release of ammonia by the activity of L-glutaminase (L-glutamine amidohydrolase EC 3.5.1. Read More

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Activation of Prp28 ATPase by phosphorylated Npl3 at a critical step of spliceosome remodeling.

Nat Commun 2021 05 25;12(1):3082. Epub 2021 May 25.

Genomics Research Center, Academia Sinica, Taipei, Taiwan.

Splicing, a key step in the eukaryotic gene-expression pathway, converts precursor messenger RNA (pre-mRNA) into mRNA by excising introns and ligating exons. This task is accomplished by the spliceosome, a macromolecular machine that must undergo sequential conformational changes to establish its active site. Each of these major changes requires a dedicated DExD/H-box ATPase, but how these enzymes are activated remain obscure. Read More

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The genotypes and virulence attributes of C. albicans isolates from oral leukoplakia.

Med Oral Patol Oral Cir Bucal 2021 May 23. Epub 2021 May 23.

Department of Microbiology Faculty of Medical Sciences University of Sri Jayewardenepura Gangodawila, Nugegoda, Sri Lanka

Background: There is a debate as to whether some types of oral leucoplakias (OL) are caused by Candida species, and whether they contribute to the malignant transformation, associated with a minority of such lesions. As no detailed population analysis of yeast isolates from OL is available, we evaluated the virulence attributes, and genotypes of 35 C. albicans from OL, and compared their genotypes with 18 oral isolates from healthy individuals. Read More

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One-step production of functional branched oligoglucosides with coupled fermentation of Pichia pastoris GS115 and Sclerotium rolfsii WSH-G01.

Bioresour Technol 2021 Sep 15;335:125286. Epub 2021 May 15.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China. Electronic address:

Endo-β-1,3-glucanase with high specific activity is a prerequisite for enzymatic preparation of valuable β-oligoglucosides. Heterologous expression in Pichia pastoris GS115 with error-prone PCR technology was implemented, and the mutant strain 7 N12 was obtained. The mutant endo-β-1,3-glucanase showed efficient specific activities for degrading curdlan (366 U mg) and scleroglucan (274. Read More

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September 2021