24,017 results match your criteria activity crude


Separation and purification of magnoflorine, spinosin and 6'''-feruloyspinosin from Ziziphi Spinosae Semen by high-speed counter-current chromatography.

J Sep Sci 2021 Apr 13. Epub 2021 Apr 13.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.

In the study, high-speed counter-current chromatography was used for separation and purification of magnoflorine, spinosin and 6'''-feruloyspinosin from Ziziphi Spinosae Semen. With n-butanol-ethyl acetate-water (2:3:5, v/v) as the optimum solvent system, about 75 mg of magnoflorine, 110 mg of spinosin and 40 mg of 6'''-feruloyspinosin were isolated from 0.5 g of crude extract of Ziziphi Spinosae Semen, with the purity of 95. Read More

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Phytochemicals from twigs of and their antioxidation kinetics of oxymyoglobin.

Nat Prod Res 2021 Apr 13:1-5. Epub 2021 Apr 13.

Natural Products Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.

The phytochemical investigation of crude -hexane and ethyl acetate extracts from twigs of (Kurz) led to the isolation of 14 known compounds. Their structures were elucidated by spectroscopic techniques (IR, H NMR, C NMR, and 2D NMR) as well as mass spectrometry. These structures were characterized as -sitosterol (), lupeol (), vanilic acid (), 5,7-dihydroxychromone (), (+)-mellein (), isoliquiritigenin (), 7-hydroxyemodin (), physion (), aromadendrin (), naringenin (), apigenin (), luteolin (), chrysoeriol () and kaempferol (). Read More

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Prevalence of lipase producer Aspergillus niger in nuts and anti-biofilm efficacy of its crude lipase against some human pathogenic bacteria.

Sci Rep 2021 Apr 12;11(1):7981. Epub 2021 Apr 12.

Faculty of Science, Botany and Microbiology Department, South Valley University, Qena, 83523, Egypt.

Nuts are the natural source of healthy lipids, proteins, and omega-3. They are susceptible to fungal and mycotoxins contamination because of their high nutritional value. Twenty-five species comprising 12 genera were isolated from 80 samples of dried fruits and nuts using the dilution plate method. Read More

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Cytochrome P450 Enzyme Inhibition and Herb-Drug Interaction Potential of Medicinal Plant Extracts Used for Management of Diabetes in Nigeria.

Eur J Drug Metab Pharmacokinet 2021 Apr 12. Epub 2021 Apr 12.

Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, 7 Greenhouse Rd, Kingston, RI, 02881, USA.

Background And Objective: The use of herbal medicines is common in Africa, and patients often use a combination of herbs and drugs. Concurrent herbal and pharmaceuticals treatments can cause adverse effects through herb-drug interactions (HDI). This study evaluated the potential risk of HDI for five medicinal plants, Vernonia amygdalina, Ocimum gratissimum, Moringa oleifera, Azadirachta indica, and Picralima nitida, using in vitro assays. Read More

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Enzyme polymer engineered structure strategy to enhance cross-linked enzyme aggregate stability: a step forward in laccase exploitation for cannabidiol removal from wastewater.

Environ Sci Pollut Res Int 2021 Apr 12. Epub 2021 Apr 12.

Université de Sherbrooke Water Research Group, Department of Civil and Building Engineering, Université de Sherbrooke, 2500 boul. de l'Université, Sherbrooke, Québec, J1K 2R1, Canada.

Despite all its advantages and potential, cross-linking enzyme aggregate (CLEA) technology is still not applied at an industrial scale for enzyme insolubilization for bioremediation purposes. In this study, the enzyme polymer engineered structure (EPES) method was used to enhance CLEA stability and reuse. A crude laccase from Trametes hirsuta was successfully insolubilized to form EPES-CLEAs. Read More

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Isolation and Identification of Antibacterial Bioactive Compounds From L2.

Front Microbiol 2021 24;12:645484. Epub 2021 Mar 24.

Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering, College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang, China.

Bacterial metabolites exhibit a variety of biologically active compounds including antibacterial and antifungal activities. It is well known that is considered to be a promising source of bioactive secondary metabolites. Most plant pathogens have an incredible ability to mutate and acquire resistance, causing major economic losses in the agricultural field. Read More

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Particle-stabilized oil foams.

Adv Colloid Interface Sci 2021 Mar 20;291:102404. Epub 2021 Mar 20.

Department of Chemistry, University of Hull, Hull HU6 7RX, UK.

The area of oil foams although important industrially has received little academic attention until the last decade. The early work using molecular surfactants for stabilisation was limited and as such it is difficult to obtain general rules of thumb. Recently however, interest has grown in the area partly fuelled by the understanding gained in the general area of colloidal particles at fluid interfaces. Read More

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Development of an effective method based upon second-order overlapping repeated sample injections for isolation of carotenoids from Lycium barbarum L. fruits with elution-extrusion counter-current chromatography.

J Chromatogr A 2021 Mar 27;1645:462026. Epub 2021 Mar 27.

CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory of Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, P. R. China; University of Chinese Academy of Sciences, Beijing, P. R. China. Electronic address:

Carotenoids are one of the main active components in Lycium barbarum L. fruit, which has a wide range of excellent biological activities. In this study, a novel second-order overlapping repeated injection method with elution-extrusion counter-current chromatography was developed for isolation and preparation of carotenoids from L. Read More

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Phytochemical analysis of medicinal plants of Nepal and their antibacterial and antibiofilm activities against uropathogenic Escherichia coli.

BMC Complement Med Ther 2021 Apr 9;21(1):116. Epub 2021 Apr 9.

Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, Nepal.

Background: A biofilm is an extracellular polymeric substance (EPS) composed of polysaccharides, proteins, nucleic acids, and lipids that impede antibiotics and immune cells, thus providing a shielded environment for bacterial growth. Due to biofilm formation, some microbes can show up to 1000 fold increased resistance towards the antibiotics than the normal planktonic forms. The study was conducted to screen the crude extracts of medicinal plants used in Nepal for their in vitro antibiofilm activities. Read More

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Preparation, characterization, antioxidant and anti-inflammatory activities of acid-soluble pectin from okra (Abelmoschus esculentus L.).

Int J Biol Macromol 2021 Apr 6. Epub 2021 Apr 6.

Engineering Research Center of Bio-Process of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, Anhui, China.

Currently, there are few studies on acid-soluble pectin from okra, especially in biological activity for antioxidant and anti-inflammatory. In this study, the antioxidant properties of acid-soluble okra pectin components and their anti-inflammatory were explored. Firstly, two acid-soluble okra pectic fractions, namely crude acid-soluble okra pectin (CAOP) and acid-soluble okra pectin (AOP), were obtained and exhibited structural and compositional variation. Read More

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The association between dietary inflammatory index, muscle strength, muscle endurance, and body composition in Iranian adults.

Eat Weight Disord 2021 Apr 8. Epub 2021 Apr 8.

Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences (TUMS), Tehran, Iran.

Purpose: Dietary factors may modulate inflammation status which contributed to the various chronic diseases like sarcopenia. Therefore, we aimed to examine the association of dietary inflammatory index (DII) and muscle strength (MS), muscle endurance (ME), and body composition.

Methods: This cross-sectional study was conducted in 270 adults living in Tehran, Iran. Read More

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Comparison of antifungal efficacy of ethanolic extracts of leaf and peel in uncontrolled diabetic patients wearing removable dentures: A randomized controlled clinical trial.

Curr Med Mycol 2020 Sep;6(3):15-20

Department of Oral Medicine and Radiology, Mamata Dental College, Khammam, Telangana, India.

Background And Purpose: The search for the development of a suitable novel antimicrobial agent for fungal diseases continues to be a key problem in the current clinical field. The present investigation was aimed to determine the antifungal effect of the ethanolic crude extracts of leaf (Wfl) and Punica granatum peel (Pgp) in uncontrolled diabetic patients wearing removable dentures.

Materials And Methods: The ethanolic extracts of both plants were prepared using the soxhlet extraction method, and the obtained metabolites were confirmed by thin- layer chromatography. Read More

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September 2020

Insight on the fractionations and structural characterizations of innovative antidiarrheal compounds screened from leaves of Psidium guajava of local origin in Pakistan.

Pak J Pharm Sci 2020 Sep;33(5(Supplementary)):2323-2329

Imran Adrees College of Pharmacy, 3Km Daska Road, Sialkot, Pakistan.

Numerous ailments have been effectively treated with natural plants for long time all over the world. Plants provided a back bone for the exploration of novel medicinal compounds. Therefore, chief focus of our study was to isolate the biologically active compounds from the plant source and evaluate their antidiarrheal potentials, as diarrhea is still the most dominant disease in developing countries. Read More

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September 2020

Antimicrobial activities of silver nanoparticles of extra virgin olive oil and sunflower oil against human pathogenic microbes.

Pak J Pharm Sci 2020 Sep;33(5(Supplementary)):2285-2291

Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena, Egypt.

Silver nanoparticles were synthesized using extra virgin olive oil (Olea europaea L.) and sunflower oil (Helianthus annuus L.) and characterized by UV-vis spectroscopy, X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). Read More

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September 2020

Phytochemical, antimicrobial and time-kill kinetics potentials of Euphorbia nivulia Buch.-Ham.: A Cholistan desert medicinal plant.

Pak J Pharm Sci 2020 Sep;33(5(Special)):2461-2469

Department of Pharmacognosy, Faculty of Pharmacy, Gomal University, Dera Ismail Khan, Pakistan.

Euphorbia nivulia a locally occurring plant species possesses antiseptic, analgesic and anti-inflammatory properties and is ethnopharmacologically used in various ailments like skin, ear disorders, boils, and worm infestation. Preliminary phytochemical screening showed presence of flavonoids, polyphenolics, glycosides, alkaloids, tannins and triterpenoids in (70% aqueous-ethanolic) Euphorbia nivulia crude extract (En cr) and its four fractions, i.e. Read More

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September 2020

Rational engineering of xylanase hyper-producing system in Trichoderma reesei for efficient biomass degradation.

Biotechnol Biofuels 2021 Apr 8;14(1):90. Epub 2021 Apr 8.

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.

Background: Filamentous fungus Trichoderma reesei has been widely used as a workhorse for cellulase and xylanase productions. Xylanase has been reported as the crucial accessory enzyme in the degradation of lignocellulose for higher accessibility of cellulase. In addition, the efficient hydrolysis of xylan needs the co-work of multiple xylanolytic enzymes, which rise an increasing demand for the high yield of xylanase for efficient biomass degradation. Read More

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Highly efficient Co single-atom catalyst for epoxidation of plant oils.

J Chem Phys 2021 Apr;154(13):131103

CAS Key Laboratory of Science and Technology on Applied Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Epoxidation of bio-derived plant oils is a sustainable route to manufacturing plasticizers, additives in lubricants, and other chemicals. The traditional synthetic approaches suffer from the employment of corrosive mineral acid or expensive peroxides (e.g. Read More

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Synergy in Efficacy of Crude Extract and on Resistant .

Front Physiol 2021 22;12:642893. Epub 2021 Mar 22.

State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

In order to explore the synergistic control effect of crude extracts of and on , we used different doses of and crude extracts of singly and in combination, to determine their toxicities to fourth instar . The results showed that the combination of 10% crude extract of with 10 and 10 spores/g concentrations and the combination of 20% crude extract of with 10 and 10 spores/g concentrations had significant effects on the mortality, body weight gain, body length gain, growth rate, and overall performance of than those of the crude extract of and alone. Among them, the 20% crude extract mixed with 10 spores/g and 10% crude extract combined with 10 spores/g , had the best control efficacy. Read More

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Biochemical and synergistic properties of a novel alpha-amylase from Chinese nong-flavor Daqu.

Microb Cell Fact 2021 Apr 7;20(1):80. Epub 2021 Apr 7.

CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, No. 9 Section 4, Renmin Nan Road, Chengdu, 610041, Sichuan, People's Republic of China.

Background: Daqu is the most important fermentation starter for Chinese liquor, with large number of microbes and enzymes being openly enriched in the Daqu system over thousands of years. However, only a few enzymes have been analyzed with crude protein for total liquefying power and saccharifying power of Daqu. Therefore, the complex enzymatic system present in Daqu has not been completely characterized. Read More

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Enzymatic diversity of filamentous fungi isolated from forest soil incremented by sugar cane solid waste.

Environ Technol 2021 Apr 7:1-21. Epub 2021 Apr 7.

Institute of Environmental, Chemical and Pharmaceutical Sciences, Universidade Federal de São Paulo, São Paulo, Brazil.

Fungi are natural degraders of organic matter which can produce enzymes for many industrial and biotechnological applications. In this context, crude enzymatic extracts of fungal isolates were evaluated regarding their hydrolytic and ligninolytic abilities. The fungal strains were isolated from soil samples from Atlantic Rain Forest Park incremented with sugar cane biomass (filter cake), which allowed the selection of efficient lignocellulolytic enzymes. Read More

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Lipase production by microorganisms isolated from the Serra de Ouro Branco State Park.

An Acad Bras Cienc 2021 31;93(1):e20190672. Epub 2021 Mar 31.

Universidade Federal de São João Del-Rei (UFSJ/ DEQUI), Departamento de Engenharia Química, Campus Alto Paraopeba, Rodovia MG 443, Km 07, 36420-000 Ouro Branco, MG, Brazil.

Lipases are hydrolases used in various sectors such as the food, pharmaceutical and chemical synthesis industries. In this study, epiphytic microorganisms were isolated from the Serra of Ouro Branco State Park (Minas Gerais, Brazil) and were subsequently evaluated for their ability to produce extracellular lipases. Among the 46 isolated strains, 25 presented positive results for lipase production in the agar plate screening assay. Read More

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Synthesis and biological evaluation of Ellettaria cardamomum (Cardamom) Phytosomes.

Pak J Pharm Sci 2020 Sep;33(5):2121-2129

Department of Chemistry, University of Agriculture, Faisalabad, Pakistan.

Ellettaria cardamomum (Cardamom) is an excellent antioxidant and its phytochemicals possess astounding detox properties. The bioactives of E. cardamomum have low bioavailability due to complex molecular structure. Read More

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September 2020

Anti-inflammatory and antibacterial activities of P. petiolosa (Christ) Ching ethyl acetate extract against S. aureus in mice.

Pak J Pharm Sci 2020 Sep;33(5):2047-2052

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, P.R. China.

P. petiolosa as a typical Chinese herbal medicine has been generally utilized as Chinese native medicine formulation for treatment of chronic bronchitis, bronchial asthma and pneumoconiosis. The objective of this study was to evaluate the anti-inflammatory and antibacterial activities of P. Read More

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September 2020

A review of the aromatic genus Adenosma: Geographical distribution, traditional uses, phytochemistry and biological activities.

J Ethnopharmacol 2021 Apr 3:114075. Epub 2021 Apr 3.

Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address:

Ethnopharmacological Relevance: The strongly scented genus Adenosma R. Brown (Plantaginaceae) comprises between 26 and 29 species with mainly southeast Asian distributions. Several species are used traditionally, mostly in Asian countries, for medicinal purposes including the treatment of colds and tumors, as well as stomach, liver, and skin disorders. Read More

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Chemical Composition and Ultraviolet Absorption Activity of an Aqueous Alkali Extract from the Fruiting Bodies of the Tinder Conk Mushroom, Fomes fomentarius (Agaricomycetes).

Int J Med Mushrooms 2021 ;23(4):23-37

Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany.

White rot mushroom Fomes fomentarius is a medicinal fungus with great potential to be explored. This work focused on the chemical composition of a basic aqueous extract from F. fomentarius fruiting bodies. Read More

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January 2021