10,321 results match your criteria activities erythrocytes


Apoptosis Induction in Murine Melanoma (B16F10) Cells by Mannosylerythritol Lipids-B; a Glycolipid Biosurfactant with Antitumoral Activities.

Appl Biochem Biotechnol 2021 Jul 24. Epub 2021 Jul 24.

Graduate Program in Chemical Engineering, Department of Chemical and Food Engineering, Federal University of Santa Catarina, Trindade, s/n, Florianópolis, Santa Catarina, 88040-900, Brazil.

Mannosylerythritol lipids have drawn attention to cosmetic and pharmaceutical industries due to their non-toxicity and excellent biological interactions with human skin, particularly with the deepest epidermal layer. Lamellar liquid crystal structure, formed by MEL-B, is an interesting feature due to its similarity to the stratum corneum molecular arrangement and cell signaling events involved in the deregulation of the cancerous cell membrane. Thus, this work aimed to evaluate the cytotoxicity of commercial mannosylerythritol lipids-B in murine melanoma, fibroblast, and human erythrocytes cells. Read More

View Article and Full-Text PDF

Unlocking the Real Potential of Black Soldier Fly () Larvae Protein Derivatives in Pet Diets.

Molecules 2021 Jul 11;26(14). Epub 2021 Jul 11.

Protix B.V., 5107 NC Dongen, The Netherlands.

Black soldier fly larvae (BSFL)-derived proteins are gaining popularity as sustainable pet food ingredients. According to the literature, these ingredients have strong antioxidant and antimicrobial activities. Due to the ability of BSFL protein derivatives to donate hydrogen atoms and/or electrons to counterpoise unstable molecules, they could possibly help in the prevention of osteoarthritis. Read More

View Article and Full-Text PDF

Protective Effect of Haustorium Extract against Alkoxyl Radical-Induced Cytotoxicity by Improving Glutathione Metabolism by Modulating Nrf2/Haeme Oxygenase-1 Expression.

J Am Coll Nutr 2021 Jul 20:1-7. Epub 2021 Jul 20.

Division of Cell and Molecular Biology, Post Graduate & Research Department of Zoology, St. Joseph's College (Autonomous), Devagiri, Calicut, Kerala, India.

Aim: The study was aimed to assess the ability of haustorium methanolic extract (BHE) on de novo glutathione biosynthesis in normal and pro-oxidant exposed cells via Nuclear factor erythroid 2-related factor 2 (Nrf2) and haeme oxygenase-1 (HO1) signaling in 2,2'-Azobis(2-methylpropionamidine) di-hydrochloride (AAPH) induced cytotoxicity in normal intestinal epithelial cells (IEC-6 cells).

Methods: The in vitro antioxidant activity was determined in terms of radical scavenging and ex vivo hemolysis. The cytoprotective effect was studied using AAP H as the alkoxyl radical inducer in IEC-6 cell model. Read More

View Article and Full-Text PDF

The role of ascorbic acid combined exposure on Imidacloprid-induced oxidative stress and genotoxicity in Nile tilapia.

Sci Rep 2021 Jul 19;11(1):14716. Epub 2021 Jul 19.

Pharmacognosy Group, Department of Pharmaceutical Biosciences, BMC, Uppsala University, Box 591, 751 24, Uppsala, Sweden.

Imidacloprid (Imid), a systemic neonicotinoid insecticide, is broadly used worldwide. It is reported to contaminate aquatic systems. This study was proposed to evaluate oxidative stress and genotoxicity of Imid on Nile tilapia (Oreochromis niloticus) and the protective effect of ascorbic acid (Asc). Read More

View Article and Full-Text PDF

Redox State and Lysosomal Activity in Women with Ovarian Cancer with Tumor Recurrence and Multiorgan Metastasis.

Molecules 2021 Jul 1;26(13). Epub 2021 Jul 1.

Department of Medical Biology and Biochemistry, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-092 Bydgoszcz, Poland.

The aim of the study is to evaluate oxidant-antioxidant balance as well as lysosomal and anti-protease activities in ovarian cancer since it has been emphasized that the crucial inducing factor of carcinogenesis may be reactive oxygen/nitrogen species or, more precisely, oxidative stress-induced inflammation. The study involved 15 women with ovarian cancer, aged 59.9 ± 7. Read More

View Article and Full-Text PDF

Evaluation of the systemic alterations triggers by Porthidium lansbergii lansbergii snake venom.

Acta Trop 2021 Jul 14;222:106047. Epub 2021 Jul 14.

Centro de Estudios Cerebrales, Facultad de Salud, Universidad del Valle, Cali, Colombia.

The systemic effects generated by Porthidium lansbergii lansbergii envenoming, a species found in the northern region of Colombia, is poorly known. The present study aimed to analyze for the first time the mice's behavior, the histological alterations, and changes in biochemical markers levels resulting from the intraperitoneal injection of an LD of P. lansbergii lansbergii snake venom on mice. Read More

View Article and Full-Text PDF

Dietary supplementation with curcumin-loaded nanocapsules in lambs: Nanotechnology as a new tool for nutrition.

Anim Nutr 2021 Jun 17;7(2):521-529. Epub 2021 Mar 17.

Department of Animal Science, Universidade Do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.

Curcumin-containing nanocapsule powder formulations have not been used in ruminant feed to date, despite the fact that curcumin is known to be a functional food additive. The objective of this study was to determine whether ethyl polymethacrylate (Eudragit L-100) nanocapsules loaded with curcumin (N-CU) would improve health and growth of lambs. Thirty-two male Lacaune lambs (body weight [BW] = 16 ± 0. Read More

View Article and Full-Text PDF

Wild garlic extract reduces lipid peroxidation in terbuthylazine-treated human erythrocytes.

Biomarkers 2021 Jul 19:1-8. Epub 2021 Jul 19.

Faculty of Agriculture, University of Banja Luka, Banja Luka, Bosnia and Herzegovina.

Among other negative effects, herbicides induce oxidative stress, leading to lipid peroxidation and protein oxidation. Therefore, there is a growing need to identify natural compounds with sufficient antioxidant capacity and mitigate the negative effects of herbicides without side effects. Our study aimed to examine the protective effect of the phenolic extract of wild garlic (WG) leaves on terbuthylazine-treated erythrocytes. Read More

View Article and Full-Text PDF

Promising effects of Rosa damascena petal extracts as antioxidant and antibacterial agents.

Pak J Pharm Sci 2021 Jan;34(1):1-8

Laboratory of Biochemistry and Molecular Genetics, Faculty of Sciences and Technologies, University Abdelmalek Essaadi Tangier, Morocco.

In the present work, the antioxidant properties of methanolic (MeOH), ethyl acetate (EtOAc) and chloroformic (CHCl) fractions of Rosa damascena petals were evaluated. Antioxidant capacity was assessed by free radical scavenging assays (DPPH•) and ferrous ions (Fe) chelating activity. Antibacterial activity was evaluated using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and IC. Read More

View Article and Full-Text PDF
January 2021

Antimalarial activities of ()-2-(nitroheteroarylmethylene)-3(2)-benzofuranone derivatives: , assessment and β-hematin formation inhibition activity.

Antimicrob Agents Chemother 2021 Jul 6:AAC0268320. Epub 2021 Jul 6.

Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14176, Iran.

A series of ()-2-(nitroheteroarylmethylene)-3(2)-benzofuranones possessing nitroheteroaryls groups of nitroimidazole, nitrofuran and nitrothiophene moieties was screened for antiplasmodium activity against drug-sensitive (3D7) as well as a chloroquine (CQ) and multi-drug resistant (K1) strains of 5-Nitroimidazole and 4-nitroimidazole analogs were highly selective and active against resistant parasites, while 5-nitrofuran and 5-nitrothiophene derivatives were more potent against the 3D7 strain than the K1 strain. Among the synthetic analogues, ()-6-chloro-2-(1-methyl-5-nitroimidazol-2-ylmethylene)-3(2)-benzofuranone () exhibited the highest activity (IC: 0.654 nM) against K1 strain and ()-7-methoxy-2-(5-nitrothiophen-2-ylmethylene)-3(2)-benzofuranone () showed the highest activity (IC: 0. Read More

View Article and Full-Text PDF

Serum Tumor Necrosis Factor-Alpha as a Competent Biomarker for Evaluation of Disease Activity in Early Rheumatoid Arthritis.

Cureus 2021 May 29;13(5):e15314. Epub 2021 May 29.

Biochemistry, Ziauddin University, Karachi, PAK.

Aim The cytokines particularly tumor necrosis factor-alpha (TNF-α) have a substantial role in the pathophysiology of rheumatoid arthritis (RA). The goal of this study was to evaluate the role of serum TNF-α as a competent biomarker of disease activity in RA and to assess the correlation of serum TNF-α with DAS28-ESR (disease activity score-erythrocyte sedimentation rate in 28 joints) and other markers expressed in serum of RA patients. Methodology The study was conducted from May 2020 to October 2020 after approval from the Ethical Review Committee of Ziauddin University. Read More

View Article and Full-Text PDF

Interplay between LPA2 and LPA3 in LPA-mediated phosphatidylserine cell surface exposure and extracellular vesicles release by erythrocytes.

Biochem Pharmacol 2021 Jun 30;192:114667. Epub 2021 Jun 30.

Centre de recherche du CHU de Québec-Université Laval, Centre ARThrite de l'Université Laval, Département de microbiologie-infectiologie et d'immunologie, Université Laval, Québec, QC G1V 4G2, Canada. Electronic address:

Evidence is growing for the role of red blood cells (RBCs) in vascular homeostasis, including thrombogenic events and inflammation. Lysophosphatidic acid (LPA) is known to induce phosphatidylserine (PS) exposure and the release of RBC Extracellular Vesicles (REVs). Using high sensitivity flow cytometry, we examined the effects and the mechanisms by which the LPA species commonly found in human plasma could activate RBCs. Read More

View Article and Full-Text PDF

Antimicrobial Activity and Protective Effect of Tuscan Bee Pollens on Oxidative and Endoplasmic Reticulum Stress in Different Cell-Based Models.

Foods 2021 Jun 18;10(6). Epub 2021 Jun 18.

National Research Council, Institute of Agricultural Biology and Biotechnology (IBBA), Via Moruzzi 1, 56124 Pisa, Italy.

Bee pollen is an apiary product of great interest owing to its high nutritional and therapeutic properties. This study aimed to assess the cellular antioxidant activity and the antihemolytic effect of , , and bee pollens on human erythrocytes. We also tested the antimicrobial potential of each sample on selected Gram-negative and Gram-positive bacteria. Read More

View Article and Full-Text PDF

Antioxidant and Anti-Inflammatory Effects of and : In Silico, Biochemical and Histological Study.

Foods 2021 Jun 15;10(6). Epub 2021 Jun 15.

Department of Biology, University of Ha'il, Ha'il 81451, Saudi Arabia.

In this study, the antioxidant and anti-inflammatory effects of and aqueous extracts were examined in a carrageenan-induced acute inflammation model. Some markers of inflammation such as hematological parameters, fibrinogen and C-reactive protein were measured. Variables reflecting oxidative stress included thiobarbituric acid reactive substances (TBARS), advanced oxidation of protein products (AOPP), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione were determined in both inflamed foci and erythrocytes. Read More

View Article and Full-Text PDF

The Design of Alapropoginine, a Novel Conjugated Ultrashort Antimicrobial Peptide with Potent Synergistic Antimicrobial Activity in Combination with Conventional Antibiotics.

Antibiotics (Basel) 2021 Jun 13;10(6). Epub 2021 Jun 13.

Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, University of Jordan, Amman 11942, Jordan.

(1) Background: Antimicrobial resistance represents an urgent health dilemma facing the global human population. The development of novel antimicrobial agents is needed to face the rising number of resistant bacteria. Ultrashort antimicrobial peptides (USAMPs) are considered promising antimicrobial agents that meet the required criteria of novel antimicrobial drug development. Read More

View Article and Full-Text PDF

Dose-Dependent Oxidative Damage in Erythrocytes and Hepatic Tissue of Wistar Rats Concurrently Exposed with Arsenic and Quinalphos: a Subacute Study.

Biol Trace Elem Res 2021 Jun 29. Epub 2021 Jun 29.

Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-J, R S Pura, 181102, Jammu & Kashmir, India.

Concurrent exposure to a multitude of environmental toxicants pose serious health hazard to humans and animals. The present investigation was conceptualized to determine deleterious effects of concomitant subacute arsenic and quinalphos exposure on antioxidant responses of liver and erythrocytes of Wistar rats. Fifty-four Wistar rats were divided into nine groups with six animals in each. Read More

View Article and Full-Text PDF

Biological responses in pesticide exposed lizards (Podarcis siculus).

Ecotoxicology 2021 Aug 26;30(6):1017-1028. Epub 2021 Jun 26.

Dipartimento di Scienze, Università Roma Tre, Viale G. Marconi 446, 00146, Rome, Italy.

The release of contaminants as herbicides, fungicides and insecticides into the environment has been listed as one of the six major contributors to the global decline of reptiles. Although reptiles may face severe risk from contaminants due to their ecology and physiology, they are currently less studied than other vertebrate groups. In the present work, we investigated if and how different types of field treatment (conventional and organic) affected the health status of Italian wall lizard (Podarcis siculus) individuals in central Italy. Read More

View Article and Full-Text PDF

Experimental Study on Plasmodium knowlesi Normocyte Binding Protein Xa Region II (PkNBPXaII) for Erythrocyte Binding.

Trop Biomed 2021 Jun;38(2):143-148

Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Normocyte binding protein Xa (NBPXa) has been implied to play a significant role in parasite invasion of human erythrocytes. Previous phylogenetic studies have reported the existence of three types of NBPXa for Plasmodium knowlesi (PkNBPXa). PkNBPXa region II (PkNBPXaII) of type 1, type 2 and type 3 were expressed on mammalian cell surface and interacted with human and macaque (Macaca fascicularis) erythrocytes. Read More

View Article and Full-Text PDF

Enhanced splenic volume and contraction in elite endurance athletes.

J Appl Physiol (1985) 2021 06 24. Epub 2021 Jun 24.

Environmental Physiology Group, Department of Health Sciences, Mid Sweden University, Östersund, Sweden.

Splenic contraction, which leads to ejection of stored erythrocytes, is greater in athletes involved in regular freediving or high-altitude activities. As this response facilitates oxygen carrying capacity, similar characteristics may be expected of elite endurance athletes. Therefore, our aims were to compare resting and apnea-induced splenic volume in endurance athletes and untrained individuals, and to assess the athletes' exercise-induced splenic volume. Read More

View Article and Full-Text PDF

Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat.

Sci Prog 2021 Apr-Jun;104(2):368504211021751

Department of Biological Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.

The aquatic ecosystem is under increasing pressure from environmental contaminants due to anthropogenic activities. This study investigated the potential of glyphosate and paraquat to induce DNA damage and other cell abnormalities in juvenile African Catfish, . Juvenile fish were exposed for 96 h to 0. Read More

View Article and Full-Text PDF

Roles of conserved residues in the receptor binding sites of human parainfluenza virus type 3 HN protein.

Microb Pathog 2021 Jun 17;158:105053. Epub 2021 Jun 17.

Department of Virology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China. Electronic address:

Human parainfluenza virus type 3 (hPIV-3) entry and intrahost spread through membrane fusion are initiated by two envelope glycoproteins, hemagglutinin-neuraminidase (HN) and fusion (F) protein. Binding of HN protein to the cellular receptor via its receptor-binding sites triggers conformational changes in the F protein leading to virus-cell fusion. However, little is known about the roles of individual amino acids that comprise the receptor-binding sites in the fusion process. Read More

View Article and Full-Text PDF

Ameliorative role of ascorbic acid on the oxidative stress and genotoxicity induced by acetamiprid in Nile tilapia (Oreochromis niloticus).

Environ Sci Pollut Res Int 2021 Jun 14. Epub 2021 Jun 14.

Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, 32511, Egypt.

On juveniles of Oreochromis niloticus, the protective potential of ascorbic acid (Asc) against oxidative stress and genotoxicity induced by acetamiprid (Aceta) sub-lethal concentrations was investigated in this study. Fishes were divided into six groups and exposed to either Asc (50 ppm), 10 and 20 ppm Aceta, 10 ppm (Aceta)+Asc, 20 ppm (Aceta)+Asc, or the unexposed control group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities and their transcripts were assessed. Read More

View Article and Full-Text PDF

Synthesis and biological evaluation of some 1-naphthol derivatives as antioxidants, acetylcholinesterase, and carbonic anhydrase inhibitors.

Arch Pharm (Weinheim) 2021 Jun 3:e2100113. Epub 2021 Jun 3.

Department of Chemistry, Faculty of Sciences, Atatürk University, Erzurum, Turkey.

A series of some naphthol derivatives 4a-f, 5a,f, 6a, and 7a,b (six novel ones: 4c,d, 5a, 6a, 7a,b) bearing F, Cl, Br, OMe, and dioxole substituents at different positions of the aromatic rings was designed, synthesized, and characterized. The naphthol derivatives were synthesized in three steps, namely the addition reaction of furan via Diels-Alder cycloaddition reaction, copper(II) trifluoromethanesulfonate (Cu(OTf) )-catalyzed aromatization reaction, and the bromination reaction, respectively. The structures of the newly obtained compounds (4c,d, 5a, 6a, 7a,b) were characterized by spectroscopic techniques. Read More

View Article and Full-Text PDF

Modulatory Effect of Chaetomorpha gracilis on Erythrocytes Functions and Metabolic Disorders in Mice Fed a High-cholesterol Diet.

J Oleo Sci 2021 ;70(6):837-848

Laboratory of Animal Physiology, Sfax Faculty of Sciences, University of Sfax.

The current study was carried out to estimate the protective effect of methanolic extract of Chaetomorpha gracilis (MECG) against High Cholesterol Diet (HCD) induced erythrocyte damage in mice. The results of the in vitro assay showed that MECG have higher antioxidant capacities in the DPPH, TAC, ABTS, NBT, NO inhibition assays. The HPLC analysis confirmed that this potential antioxidant seems to be due to the active compounds, in particular polyphenols, flavonoids. Read More

View Article and Full-Text PDF
January 2021

8a, a New Acridine Antiproliferative and Pro-Apoptotic Agent Targeting HDAC1/DNMT1.

Int J Mol Sci 2021 May 24;22(11). Epub 2021 May 24.

Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China.

Epigenetic therapy using histone deacetylase (HDAC) inhibitors has become an attractive project in new drug development. However, DNA methylation and histone acetylation are important epigenetic ways to regulate the occurrence and development of leukemia. Given previous studies, -(2-aminophenyl)benzamide acridine (), as a histone deacetylase 1 (HDAC1) inhibitor, induces apoptosis and shows significant anti-proliferative activity against histiocytic lymphoma U937 cells. Read More

View Article and Full-Text PDF

The Pro-Oxidant Activity of Red Wine Polyphenols Induces an Adaptive Antioxidant Response in Human Erythrocytes.

Antioxidants (Basel) 2021 May 18;10(5). Epub 2021 May 18.

National Research Council, Institute of Food Sciences, 83100 Avellino, Italy.

The protective effect of dealcoholized red wine on human health has been partially associated with its polyphenolic components, suggesting that the pool of polyphenols, including flavonoids and anthocyanins, can be responsible for the functional effects of this beverage. We hypothesize a new role of red wine polyphenols (RWp) in modulating the antioxidant potential of erythrocytes, protecting them against oxidative stress. We previously demonstrated that RWp activated the Plasma Membrane Redox System (PMRS), which is involved in neutralizing plasma free radicals. Read More

View Article and Full-Text PDF

Urolithin A and B Alter Cellular Metabolism and Induce Metabolites Associated with Apoptosis in Leukemic Cells.

Int J Mol Sci 2021 May 22;22(11). Epub 2021 May 22.

Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Leukemia is persistently a significant cause of illness and mortality worldwide. Urolithins, metabolites of ellagic acid and ellagitannins produced by gut microbiota, showed better bioactive compounds liable for the health benefits exerted by ellagic acid and ellagitannins containing pomegranate and walnuts. Here, we assessed the potential antileukemic activities of both urolithin A and urolithin B. Read More

View Article and Full-Text PDF

A C-Type Lectin Highly Expressed in Intestine Functions in AMP Regulation and Prophenoloxidase Activation.

Antibiotics (Basel) 2021 May 7;10(5). Epub 2021 May 7.

School of Marine Science, Ningbo University, Ningbo 315211, China.

A C-type lectin (PtCLec2) from was identified for characterization of its role in defense and innate immunity. PtCLec2 contains a single carbohydrate-recognition domain (CRD) with a conserved QPD motif, which was predicted to have galactose specificity. The mRNA expression of PtCLec2 was predominantly detected in intestine and increased rapidly and significantly upon pathogen challenge. Read More

View Article and Full-Text PDF

Antimicrobial Activities of LL-37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate against , , and .

Int J Mol Sci 2021 May 12;22(10). Epub 2021 May 12.

Faculty of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien 9-Bancho, Nishinomiya City 663-8179, Japan.

Various peptides and their derivatives have been reported to exhibit antimicrobial activities. Although these activities have been examined against microorganisms, novel methods have recently emerged for conjugation of the biomaterials to improve their activities. Here, we prepared CKR12-PLGA, in which CKR12 (a mutated fragment of human cathelicidin peptide, LL-37) was conjugated with poly (lactic-co-glycolic) acid (PLGA), and compared the antimicrobial and antifungal activities of the conjugated peptide with those of FK13 (a small fragment of LL-37) and CKR12 alone. Read More

View Article and Full-Text PDF

Synthesis, Physicochemical Characteristics and Plausible Mechanism of Action of an Immunosuppressive Isoxazolo[5,4-e]-1,2,4-Triazepine Derivative (RM33).

Pharmaceuticals (Basel) 2021 May 15;14(5). Epub 2021 May 15.

Laboratory of Immunobiology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 Weigla Street, 53-114 Wroclaw, Poland.

Previous studies demonstrated strong anti-inflammatory properties of isoxazolo[5,4-e]-1,2,4-triazepine (RM33) in vivo. The aim of this investigation was to describe synthesis, determine physicochemical characteristics, evaluate biological activities in murine and human in vitro models, as well as to propose mechanism of action of the compound. The compound was devoid of cell toxicity up to 100 μg/mL against a reference A549 cell line. Read More

View Article and Full-Text PDF