38 results match your criteria activation reinjection

Anomeric Retention of Carbohydrates in Multistage Cyclic Ion Mobility (IMS): De Novo Structural Elucidation of Enzymatically Produced Mannosides.

Anal Chem 2021 04 8;93(15):6254-6261. Epub 2021 Apr 8.

INRAE, UR BIA, F-44316 Nantes, France.

Carbohydrates are complex structures that still challenge analysts today because of their different levels of isomerism, notably the anomerism of the glycosidic bond. It has been shown recently that anomerism is preserved upon gas-phase fragmentation and that high-resolution ion mobility (IMS) can distinguish anomers. However, these concepts have yet to be applied to complex biological products. Read More

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Mannose-Binding Lectin Possesses Agglutination Activity and Promotes Opsonophagocytosis of Macrophages with Calreticulin Interaction in an Early Vertebrate.

J Immunol 2020 12 16;205(12):3443-3455. Epub 2020 Nov 16.

Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Institute of Modern Aquaculture Science and Engineering, School of Life Sciences, South China Normal University, Guangdong 510631, People's Republic of China; and

The innate immune system is an ancient defense system in the process of biological evolution, which can quickly and efficiently resist pathogen infection. In mammals, mannose-binding lectin (MBL) is a key molecule in the innate immune and plays an essential role in the first line of host defense against pathogenic bacteria. However, the evolutionary origins and ancient roles of immune defense of MBL and its mechanism in clearance of microbial pathogens are still unclear, especially in early vertebrates. Read More

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December 2020

Development of thermosensitive resiquimod-loaded liposomes for enhanced cancer immunotherapy.

J Control Release 2021 Feb 13;330:1080-1094. Epub 2020 Nov 13.

Molecular Imaging Program, Department of Radiology, Stanford University, 3165 Porter Drive, Palo Alto, CA 94304, USA. Electronic address:

Resiquimod (R848) is a toll-like receptor 7 and 8 (TLR7/8) agonist with potent antitumor and immunostimulatory activity. However, systemic delivery of R848 is poorly tolerated because of its poor solubility in water and systemic immune activation. In order to address these limitations, we developed an intravenously-injectable formulation with R848 using thermosensitive liposomes (TSLs) as a delivery vehicle. Read More

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February 2021

Activation of 4-1BBL+ B cells with CD40 agonism and IFNγ elicits potent immunity against glioblastoma.

J Exp Med 2021 Jan;218(1)

Department of Neurological Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL.

Immunotherapy has revolutionized the treatment of many tumors. However, most glioblastoma (GBM) patients have not, so far, benefited from such successes. With the goal of exploring ways to boost anti-GBM immunity, we developed a B cell-based vaccine (BVax) that consists of 4-1BBL+ B cells activated with CD40 agonism and IFNγ stimulation. Read More

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January 2021

MCTR1 enhances the resolution of lipopolysaccharide-induced lung injury through STAT6-mediated resident M2 alveolar macrophage polarization in mice.

J Cell Mol Med 2020 09 5;24(17):9646-9657. Epub 2020 Aug 5.

Department of Anesthesia and Critical Care, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Zhejiang, China.

Acute respiratory distress syndrome (ARDS) is a fatal disease characterized by excessive infiltration of inflammatory cells. MCTR1 is an endogenously pro-resolution lipid mediator. We tested the hypothesis that MCTR1 accelerates inflammation resolution through resident M2 alveolar macrophage polarization. Read More

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September 2020

Differential role of lipoteichoic acids isolated from Staphylococcus aureus and Lactobacillus plantarum on the aggravation and alleviation of atopic dermatitis.

Microb Pathog 2020 Oct 29;147:104360. Epub 2020 Jul 29.

Graduate School of Biotechnology, Kyung Hee University, Yongin, 17104, Republic of Korea; Research & Development Center, Skin Biotechnology Center Inc., Yongin, 17104, Republic of Korea. Electronic address:

Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, up-regulates inflammatory cytokine production through the toll-like receptor 2 (TLR2) signaling pathway, and also contributes to anti-inflammatory responses against immune cells stimulated by lipopolysaccharides. In the current study, we examined the effects of LTAs isolated from Staphylococcus aureus (aLTA) and Lactobacillus plantarum (pLTA) on the aggravation and alleviation of atopic dermatitis (AD). aLTA strongly induced CCL2 production in THP-1 cells. Read More

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October 2020

Functional Relevance of the Anaphylatoxin Receptor C3aR for Platelet Function and Arterial Thrombus Formation Marks an Intersection Point Between Innate Immunity and Thrombosis.

Circulation 2018 10;138(16):1720-1735

Department of Cardiology and Cardiovascular Medicine, University Clinic (R.J.S., H.N., P.M., T.G., O.B., M.G., H.F.L.), Eberhard Karls-University Tübingen, Germany.

Background: Platelets have distinct roles in the vascular system in that they are the major mediator of thrombosis, critical for restoration of tissue integrity, and players in vascular inflammatory conditions. In close spatiotemporal proximity, the complement system acts as the first line of defense against invading microorganisms and is a key mediator of inflammation. Whereas the fluid phase cross-talk between the complement and coagulation systems is well appreciated, the understanding of the pathophysiological implications of such interactions is still scant. Read More

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October 2018

Eculizumab treatment in severe pediatric STEC-HUS: a multicenter retrospective study.

Pediatr Nephrol 2018 08 23;33(8):1385-1394. Epub 2018 Mar 23.

Service de Néphrologie Pédiatrique, Hôpital des enfants, CHU Purpan, Toulouse, France.

Background: Hemolytic uremic syndrome related to Shiga-toxin-secreting Escherichia coli infection (STEC-HUS) remains a common cause of acute kidney injury in young children. No specific treatment has been validated for this severe disease. Recently, experimental studies highlight the potential role of complement in STEC-HUS pathophysiology. Read More

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P2y12 Receptor Promotes Pressure Overload-Induced Cardiac Remodeling via Platelet-Driven Inflammation in Mice.

Hypertension 2017 10 21;70(4):759-769. Epub 2017 Aug 21.

From the Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Hubei Key Laboratory of Genetics and Molecular Mechanism of Cardiologic Disorders, Wuhan, China.

Inflammation plays a critical role in adverse cardiac remodeling and heart failure. The P2y12 receptor is one of the predominant activating receptors for platelets, thus initiating inflammatory responses under various diseases. In this study, we investigated the functional significance of P2y12-mediated platelet activation in pressure overload-induced cardiac remodeling. Read More

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October 2017

α- L-iduronidase gene-based therapy using the phiC31 system to treat mucopolysaccharidose type I mice.

J Gene Med 2015 Jan-Feb;17(1-2):1-13

Department of Biophysics, Universidade Federal de São Paulo, São Paulo, Brazil.

Background: Mucopolysaccharidose type I (MPSI) is a lysosomal monogenic disease caused by mutations in the gene for α- L-iduronidase (IDUA). MPSI patients need a constant supply of IDUA to alleviate progression of the disease. IDUA gene transfer using integrative vectors might provide a definitive solution and support advancement to clinical trials, although studies have not yet been satisfactory. Read More

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December 2015

Updating ECP action mechanisms.

Farhad Heshmati

Transfus Apher Sci 2014 Jun 13;50(3):330-9. Epub 2014 Apr 13.

Cochin Hospital, Paris, France. Electronic address:

Extracorporeal Photochemotherapy (ECP) consists in illumination of the patient's leukocytes in the presence of 8-Methoxy Psoralen (8-MOP) and its reinjection to the same patient. ECP is responsible for many cellular events, the most important being the induction of cell apoptosis. Apoptosis appears first in lymphocytes and activated lymphocytes (allo or auto) which are more sensitive and undergo faster apoptosis rather than other cells. Read More

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Experiences with citrate rather than heparin as an anticoagulant for ¹⁸F-FDG labeling of leukocytes.

J Nucl Med Technol 2011 Dec 9;39(4):312-6. Epub 2011 Nov 9.

Department of Nuclear Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway.

Unlabelled: In all reported clinical studies with (18)F-FDG-labeled leukocytes, heparin was used as an anticoagulant during labeling. Theoretically, the substitution of heparin with citrate should be advantageous.

Methods: Blood from healthy controls was sampled in duplicate, anticoagulated with citrate or heparin, and labeled with (18)F-FDG, and the labeling yield was measured. Read More

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December 2011

Enhanced genetic instability and dasatinib sensitivity in mammary tumor cells lacking NEDD9.

Cancer Res 2010 Nov 12;70(21):8907-16. Epub 2010 Oct 12.

Departments of Developmental Therapeutics, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.

Elevated expression of the NEDD9/HEF1/Cas-L scaffolding protein promotes tumor cell invasion and metastasis in multiple cancer cell types. Conversely, generation of mammary tumors in the mouse mammary tumor virus (MMTV)-polyoma virus middle T (PyVT) genetic model is delayed by a Nedd9(-/-) genotype. These activities arise from the role of NEDD9 in assembling complexes and supporting activity of cancer signaling proteins, including FAK, Src, Shc, and AKT, and would support evaluation of NEDD9 expression as an unambiguous biomarker for tumor aggressiveness. Read More

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November 2010

Evaluation of feline monocyte-derived dendritic cells loaded with internally inactivated virus as a vaccine against feline immunodeficiency virus.

Clin Vaccine Immunol 2008 Mar 23;15(3):452-9. Epub 2008 Jan 23.

Retrovirus Center and Virology Section, Department of Experimental Pathology, University of Pisa,Via del Brennero 2, I-56127 Pisa, Italy.

Dendritic cells are the only antigen-presenting cells that can present exogenous antigens to both helper and cytolytic T cells and prime Th1-type or Th2-type cellular immune responses. Given their unique immune functions, dendritic cells are considered attractive "live adjuvants" for vaccination and immunotherapy against cancer and infectious diseases. The present study was carried out to assess whether the reinjection of autologous monocyte-derived dendritic cells loaded with an aldithriol-2-inactivated primary isolate of feline immune deficiency virus (FIV) was able to elicit protective immune responses against the homologous virus in naive cats. Read More

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Melanoma-infiltrating dendritic cells induce protective antitumor responses mediated by T cells.

Melanoma Res 2007 Jun;17(3):169-76

Louis Jeantet Skin Cancer Laboratory, Department of Dermatology, Geneva University Hospital, Geneva, Switzerland.

Dendritic cells are the most potent antigen-presenting cells inducing innate and adaptive immune response. Dendritic cells infiltrate melanomas, but their ability to induce host antitumor immunity remains obscure. In a previous study, we have observed that melanoma-infiltrating dendritic cells have the capacity to process antigens and migrate to lymph nodes to prime T lymphocytes. Read More

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Nonlinear time series analysis of jerk congenital nystagmus.

J Comput Neurosci 2006 Oct 26;21(2):153-70. Epub 2006 May 26.

The School of Mathematics, The University of Manchester, Manchester, UK.

Nonlinear dynamics provides a complementary framework to control theory for the quantitative analysis of the oculomotor control system. This paper presents a number of findings relating to the aetiology and mechanics of the pathological ocular oscillation jerk congenital nystagmus (jerk CN). A range of time series analysis techniques were applied to recorded jerk CN waveforms, and also to simulated jerk waveforms produced by an established model in which the oscillations are a consequence of an unstable neural integrator. Read More

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October 2006

Dedicated pinhole SPECT of intestinal neutrophil recruitment in a mouse model of dextran sulfate sodium-induced colitis.

J Nucl Med 2005 Mar;46(3):526-31

Department of Nuclear Medicine, Academic Medical Center, Amsterdam, The Netherlands.

Unlabelled: Evaluating the efficacy of therapy in experimental inflammatory bowel disease (IBD) requires information about inflammatory activity in bowel segments or leukocyte recruitment and about kinetics in the follow-up of treatment. This study evaluated a noninvasive scintigraphic technique able to assess neutrophil trafficking in a mouse model of dextran sulfate sodium (DSS)-induced colitis.

Methods: Groups of 4 BALB/c mice were assessed at baseline and after 1, 3, 5, and 8 d of treatment with DSS. Read More

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Suppression of CD4+ T lymphocyte effector functions by CD4+CD25+ cells in vivo.

J Immunol 2004 Mar;172(6):3391-8

Institut National de la Santé et de la Recherche Médicale Unité 561, Hôpital Saint Vincent de Paul, Paris, France.

CD4+CD25+ regulatory T cells have been extensively studied during the last decade, but how these cells exert their regulatory function on pathogenic effector T cells remains to be elucidated. Naive CD4+ T cells transferred into T cell-deficient mice strongly expand and rapidly induce inflammatory bowel disease (IBD). Onset of this inflammatory disorder depends on IFN-gamma production by expanding CD4+ T cells. Read More

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Targeting adult and pediatric cancers via cell-based vaccines and the prospect of activated B lymphocytes as a novel modality.

Cancer Biol Ther 2003 Sep-Oct;2(5):466-70

Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania USA.

The delivery of cell-based vaccines that exploit natural mechanisms of antigen presentation represents a promising approach for immunotherapy of cancer. This strategy tests the hypothesis that ex vivo manipulation and reinjection of cellular products can induce immune responses and circumvent immune incompetence to achieve clinically significant results. Whole or modified tumor cell vaccines and antigen-loaded dendritic cells (DC) are under intense clinical and scientific evaluation, but other cell-based approaches including the use of activated B lymphocytes have shown promise in preclinical in vitro models. Read More

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Release of the Ca(2+) oscillation-inducing sperm factor during mouse fertilization.

Dev Biol 2003 Aug;260(2):536-47

Department of Veterinary and Animal Science, University of Massachusetts, Amherst, MA 01003, USA.

A cytosolic sperm protein(s), referred to as the sperm factor (SF), is thought to induce intracellular calcium ([Ca(2+)](i)) oscillations during fertilization in mammalian eggs. These oscillations, which are responsible for inducing complete egg activation, persist for several hours. Nevertheless, whether a protracted release of SF is responsible for the duration of the oscillations is unknown. Read More

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Immunotherapy of established tumors using bone marrow transplantation with antigen gene--modified hematopoietic stem cells.

Nat Med 2003 Jul;9(7):952-8

Immunology & Hematopoiesis Division, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA.

A major focus of cancer immunotherapy is to develop strategies to induce T-cell responses through presentation of tumor antigens by dendritic cells (DCs). Current vaccines are limited in their ability to efficiently transfer antigens to DCs in vivo. Ex vivo-generated DCs can be efficiently loaded with antigen but after reinjection, few DCs traffic to secondary lymphoid organs, the critical sites for antigen presentation. Read More

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Candida albicans mannan-protein conjugate as vaccine candidate.

Immunol Lett 2003 Feb;85(3):251-5

Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38, Bratislava, Slovakia.

Mannan-branched polysaccharide with alpha-(1-->6)-linked mannose residues in the linear backbone, is a major virulence and protective factor of yeast Candida albicans. Injected alone does not induce sufficient level of protective antibodies. In this study, we have conjugated mannan to protein carrier by simple one step reaction using 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) activation reagent. Read More

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February 2003

The hormonal control of birth behavior in the gray short-tailed opossum (Monodelphis domestica).

R Rose B H Fadem

Horm Behav 2000 Mar;37(2):163-7

School of Zoology, University of Tasmania, Hobart, Tasmania, 7001, Australia.

In all major groups of Australian marsupials, prostaglandin F2alpha (PGF) or oxytocin injection initiates birth behavior in adult females, adult males and pouch young. Because inhibitors of PGF synthesis block this initiation, oxytocin may activate birth behavior via the stimulation of PGF synthesis. In this study, the role of PGF and oxytocin in the activation of birth behavior was examined in an American marsupial, the gray short-tailed opossum (Monodelphis domestica). Read More

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Evaluation of fluorescein-labeled autologous leukocytes for examination of retinal circulation in humans.

Curr Eye Res 2000 Jul;21(1):560-5

Departments of Ophthalmology, Nuclear Medicine and Immunohematology, Assistance Publique-Hôpitaux de Paris and Université Paris 7, H opital Lariboisière, and Laboratoire d'Etude de la Microcirculation, Paris, France.

Purpose: Increased leukocyte-endothelium interaction have been suggested as a phenomenon contributing to capillary occlusion and/or rupture of the blood-retina barrier during human retinal vascular diseases. This study was performed to evaluate if fluorescein-labeled autologous leukocytes (FLALs) can be used for examination of leukocyte transit in the human retina.

Methods: The preparation consisted of human dextran-separated leukocytes mixed with fluorescein. Read More

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Extended blood half-life of monomethoxypolyethylene glycol-conjugated hen lysozyme is a key parameter controlling immunological tolerogenicity.

Cell Mol Life Sci 1999 Jul;55(8-9):1187-94

Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.

The blood half-life of a protein is prolonged by conjugating a protein with a linear amphiphilic polymer, monomethoxypolyethylene glycol (mPEG). The conjugation gives a protein immunotolerogenicity; hence, it is likely that the long half-life is crucial for the tolerogenicity. We prepared a tolerogenic mPEG conjugate of hen egg lysozyme (mPEG1. Read More

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Without prior stimulation, tumor-associated lymphocytes from malignant effusions lyse autologous tumor cells in the presence of bispecific antibody HEA125xOKT3.

Clin Cancer Res 1999 Jan;5(1):171-80

Department of Molecular Immunology, German Cancer Research Center, Heidelberg.

Women suffering from advanced stage ovarian or mammary carcinoma frequently develop malignant ascites or pleural effusions consisting of tumor cells and tumor-associated lymphocytes (TALs). Locoregional immunotherapy with bispecific antibodies (bsAbs), which retarget T cells to tumor cells and induce their lysis, has been applied as an adjuvant treatment in the late stage of the disease. Until now, most of these therapies use peripheral blood mononuclear cells (PBMCs) as effector cells that have been stimulated and expanded ex vivo and loaded with bsAb before reinjection. Read More

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January 1999

Role of two series of Ca2+ oscillations in activation of ascidian eggs.

Dev Biol 1998 Nov;203(1):122-33

Molecular Neurobiology Laboratory, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Ibaragi, Tsukuba, 305-0074, Japan.

Changes in [Ca2+]i are an essential factor regulating egg activation. Matured ascidian eggs are arrested at metaphase I, and two series of [Ca2+]i transients have been observed after fertilization: Ca2+ waves just after fertilization (Series I) and [Ca2+]i oscillation between the first and second polar body extrusion (Series II). We investigated mechanisms involved in the elevation of [Ca2+]i and the role of the [Ca2+]i transients during egg activation in Ciona savignyi. Read More

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November 1998

Role of complement in rats injected with liposome-encapsulated hemoglobin.

J Surg Res 1997 Mar;68(2):99-105

Radiology Department, University of Texas Health Science Center at San Antonio 78284, USA.

Previous studies have documented that liposome-encapsulated hemoglobin (LEH) can cause a rapid and transient thrombocytopenia following intravenous injection into small animals. The present study evaluated the role of complement during the LEH-induced thrombocytopenia in rats. We have compared changes in platelet levels in the blood, platelet organ distribution, and total hemolytic complement levels following intravenous administration of LEH in control and complement-depleted rats. Read More

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In vivo labeling of neutrophils using a fluorescent cell linker.

J Leukoc Biol 1996 May;59(5):631-8

Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.

To study neutrophil circulation time and trafficking in vivo requires labeling the cells so that their movement can be followed temporally and spatially. Labeling procedures used to date, however, have relied on ex vivo separation and labeling methods, an undesired consequence of which may be neutrophil activation. Moreover, the labeled cells preferentially stick in the pulmonary circulation for several hours upon reinjection into the host. Read More

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Extracorporeal photochemotherapy treatment for acute lung rejection episode.

J Heart Lung Transplant 1995 Jul-Aug;14(4):793-6

Blood Bank, Hôtel-Dieu Hospital, Broussais, France.

Background And Methods: We investigated extracorporeal photochemotherapy--which consists of the collection of blood mononuclear cells by means of a cell separator, their exposure to ultraviolet A light in the presence of a photoactivatable molecule such as 8-methoxypsoralen, and their intravenous reinjection into the patient--for the treatment of an acute lung rejection episode in a severely infected patient, assuming that its mechanism of action is an immunomodulation rather than an actual immunosuppression.

Results: Three weeks after the simultaneous beginning of antiinfectious and extracorporeal photochemotherapy treatments, the patient improved clinically. Acute lung rejection was no longer detectable histologically 4 weeks after the beginning of extracorporeal photochemotherapy. Read More

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December 1995