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J Pharmacol Exp Ther 2022 Jul 2. Epub 2022 Jul 2.

Pharmacology & Molecular Therapeutics, Uniformed Services University of the Health Sciences, United States

Commonly utilized pain therapeutics like opioid medications exert dangerous side effects and lack effectiveness in treating some types of pain. Ketamine is also used to treat pain, but side effects limit its widespread use. (2,6)-hydroxynorketamine (HNK) is a ketamine metabolite that potentially shares some beneficial behavioral effects of its parent drug without causing significant side effects. Read More

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Mol Pharmacol 2022 Jul 2. Epub 2022 Jul 2.

School of Life Sciences, University of Nottingham, United Kingdom

Activation of the G protein-coupled receptors by agonists may result in the activation of one or more G proteins and recruitment of arrestins. The extent of the activation of each of these pathways depends on the intrinsic efficacy of the ligand. Quantification of intrinsic efficacy relative to a reference compound is essential for the development of novel compounds. Read More

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Network pharmacology study to reveal active compounds of Qinggan Yin formula against pulmonary inflammation by inhibiting MAPK activation.

J Ethnopharmacol 2022 Jun 29:115513. Epub 2022 Jun 29.

State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China; Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China. Electronic address:

Ethnopharmacological Relevance: Pneumonia is common and frequently-occurred disease caused by pathogens which predisposes to lung parenchymal inflammation leading pulmonary dysfunction. To prevent and alleviate the symptoms of pneumonia, Qinggan Yin formula (QGY) was composed based on clinical experience and four classical traditional Chinese medicine prescriptions which frequently applied to treat infectious diseases.

Aim Of The Study: Traditional Chinese medicine is a complex mixture and it is difficult to distinguish the effective component molecules. Read More

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DFO treatment protects against depression-like behaviors and cognitive impairment in CUMS mice.

Brain Res Bull 2022 Jun 29. Epub 2022 Jun 29.

College of Life Sciences and Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, 300071 Tianjin, PR China. Electronic address:

Depression has several negative effects on emotion as well as learning and memory abilities. Previous studies showed that depression could exacerbate inflammation, which in turn further aggravated depression. Deferoxamine (DFO) is a chelating agent binding iron and aluminium, and is clinically applied to treat acute ion poisoning and hemochromatosis. Read More

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Interleukin-2 inducible T cell kinase (ITK) may participate in the anti-bacterial immune response of Nile tilapia via regulating T-cell activation.

Fish Shellfish Immunol 2022 Jun 29. Epub 2022 Jun 29.

State Key Laboratory of Estuarine and Coastal Research, School of Life Sciences, East China Normal University, Shanghai, 200241, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.

Interleukin-2 inducible T cell kinase (ITK) plays a predominant role in the T-cell receptor (TCR) signaling cascade to ensure valid T-cell activation and function. Nevertheless, whether it regulates T-cell response of early vertebrates remains unknown. Herein, we investigated the involvement of ITK in the lymphocyte-mediated adaptive immune response, and its regulation to T-cell activation in the Nile tilapia Oreochromis niloticus. Read More

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Protective effects of NAMPT or MAPK inhibitors and NaR on Wallerian degeneration of mammalian axons.

Neurobiol Dis 2022 Jun 29:105808. Epub 2022 Jun 29.

Departments of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address:

Wallerian degeneration (WD) is a conserved axonal self-destruction program implicated in several neurological diseases. WD is driven by the degradation of the NAD synthesizing enzyme NMNAT2, the buildup of its substrate NMN, and the activation of the NAD degrading SARM1, eventually leading to axonal fragmentation. The regulation and amenability of these events to therapeutic interventions remain unclear. Read More

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Ambient NO exposure induces migraine in rats: Evidence, mechanisms and interventions.

Sci Total Environ 2022 Jun 29:157102. Epub 2022 Jun 29.

Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha 410078, PR China. Electronic address:

Migraine is a complex neurological disorder with a high disability rate. Although the precipitating factors of migraine remain unclear, previous studies suggest that when there is excess nitrogen dioxide (NO) pollution in the atmosphere, the medical demand due to migraine attacks increases sharply. However, the main role of NO as a trigger for migraine is not yet well understood. Read More

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Dexamethasone attenuates dry eye-induced pyroptosis by regulating the KCNQ1OT1/miR-214 cascade.

Steroids 2022 Jun 29:109073. Epub 2022 Jun 29.

Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Harbin, China; Key Laboratory of Basic and Clinical Research of Heilongjiang Province, Harbin, China. Electronic address:

Dry eye disease (DED) is an inflammatory disorder of the ocular surface seriously affecting the quality of life of patients. Topical dexamethasone (Dex) administration protects the cornea from the hyperosmotic stress (HS) induced by tears. Pyroptosis participates in the activation of epithelial inflammation during DED. Read More

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oReduced astrocytic mGluR5 in the hippocampus is associated with stress-induced depressive-like behaviors in mice.

Neurosci Lett 2022 Jun 29:136766. Epub 2022 Jun 29.

Department of Neurobiology and Department of General Intensive Care Unit of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China; NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou 310058, China; National Health and Disease Human Brain Tissue Resource Center, Hangzhou 310058, China. Electronic address:

Major depressive disorder (MDD) is one of the most common and disabling mental disorders that characterized by profound disturbances in emotional regulation, motivation, cognition, and the physiology of affected individuals. Although MDD was initially thought to be primarily triggered through neuronal dysfunction, the pathological alterations in astrocytic function have been previously reported in MDD. We report that chronic restraint stress (CRS) induces astrocyte activation and decreases expression of astrocytic mGluR5 in the hippocampal CA1 of susceptible mice exhibited depressive-like behaviors. Read More

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Ce(Ⅲ) activates peroxymonosulfate for the degradation of substituted PAHs.

Chemosphere 2022 Jun 29:135525. Epub 2022 Jun 29.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Substituted polycyclic aromatic hydrocarbons (SPAHs) are being intensively investigated, considering their high toxicity. Additionally, the mechanism of the effect of substituents on the removal of SPAHs and the activation of Ce(III) ions on peroxymonosulfate (PMS) have not been explored. Here we evaluated the removal efficiency of SPAHs in the oxidation system constructed by Ce(Ⅲ) ions and PMS, with emphasized the effect of substituents on SPAHs degradation. Read More

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Porous boron nitride intercalated zero-valent iron particles for highly efficient elimination of organic contaminants and Cr (VI).

Chemosphere 2022 Jun 29:135501. Epub 2022 Jun 29.

National Engineering Lab of Textile Fiber Materials & Processing Technology (Zhejiang), Zhejiang Sci-Tech University, Hangzhou, 310018, PR China; Zhejiang Provincial Innovation Center of Advanced Textile Technology, Shaoxing, 312000, PR China. Electronic address:

Developing novel bifunctional materials to high efficiently degrade organic pollutants and eliminate hexavalent chromium (Cr (VI)) is significantly desired in the wastewater treatment field. The porous boron nitride (p-BN) was fabricated by a two-stage calcination strategy and was innovatively employed to support zero-valent iron (ZVI), achieving the bifunctional material ([email protected]) to degrade carbamazepine (CBZ) and eliminate Cr (VI). [email protected] could degrade more than 98% CBZ in 6 min with the high apparent first-order constant (k) of 0. Read More

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Selenium-sulfur functionalized biochar as amendment for mercury-contaminated soil: High effective immobilization and inhibition of mercury re-activation.

Chemosphere 2022 Jun 29:135552. Epub 2022 Jun 29.

School of Metallurgy and Environment, Central South University, Changsha, 410083, PR China; Chinese National Engineering Research Centre for Control & Treatment of Heavy Metal Pollution, Changsha, 410083, PR China. Electronic address:

The contamination of soils by mercury (Hg) seriously threatens the local ecological environment and public health. S-functionalized amendments are common remediation technology, yet, Hg re-activation often occurs in the commonly used immobilization remediation by S-functionalized amendments, resulting in an unsatisfactory remediation effect. In this study, a novel FeS-Se functionalized biochar composite (FeS-Se-BC) amendment was prepared and applied for the efficient remediation of Hg-polluted soil. Read More

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The Double Knockdown of the Mitochondrial Uncoupling Protein Isoforms Reveals Partial Redundant Roles During Arabidopsis thaliana Vegetative and Reproductive Development.

Plant Sci 2022 Jun 29:111365. Epub 2022 Jun 29.

Departamento de Ciências Químicas e Biológicas (Setor Genética), Instituto de Biociências, UNESP, CEP 18618-689, Botucatu, SP, Brasil. Electronic address:

Mitochondrial uncoupling proteins (UCPs) are specialized proteins capable of dissipating the proton electrochemical gradient generated in respiration independent of ATP synthesis. Three UCP coding genes with distinct expression patterns have been identified in Arabidopsis thaliana (namely UCP1, UCP2 and UCP3). Here, we generated T-DNA double-insertion mutants (ucp1 ucp2, ucp1 ucp3 and ucp2 ucp3) to investigate the functionality of the Arabidopsis UCP isoforms. Read More

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Epicutaneous allergen immunotherapy induces a profound and selective modulation in skin dendritic cell subsets.

J Allergy Clin Immunol 2022 Jun 29. Epub 2022 Jun 29.

CIRI-Centre International de Recherche en Infectiologie; INSERM, U1111; Univ Lyon; Université de Lyon 1; Ecole Normale Supérieure de Lyon; CNRS, UMR 5308, Lyon, France. Electronic address:

Background: Epicutaneous immunotherapy (EPIT) protocols have recently been developed to restore tolerance in patients with food allergy (FA). The mechanisms by which EPIT protocols promote desensitization rely on a profound immune deviation of pathogenic T and B cell responses.

Objective: To date, little is known about the contribution of skin dendritic cells (skDCs) to T cell remodeling and EPIT efficacy. Read More

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Identification and characterization of TGF-β1-responsive Runx2 acetylation sites for matrix Metalloproteinase-13 expression in osteoblastic cells.

Biochimie 2022 Jun 29. Epub 2022 Jun 29.

Department of Biotechnology, College of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur, 603 203, Tamil Nadu, India. Electronic address:

In skeletal tissues, transforming growth factor-beta 1 (TGF-β1) serves a number of activities. For example, in osteoblastic cells, TGF-β1 stimulates the expression of matrix metalloproteinase-13 (MMP-13, a bone remodeling gene), which requires the bone transcription factor Runx2. Although TGF-β1 is known to stimulate Runx2 acetylation, the sites involved in MMP-13 gene activation remain unknown. Read More

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Single-cell RNA sequencing analysis of T helper cell differentiation and heterogeneity.

Biochim Biophys Acta Mol Cell Res 2022 Jun 29:119321. Epub 2022 Jun 29.

Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. Electronic address:

Single-cell transcriptomics has emerged as a powerful tool to investigate cells' biological landscape and focus on the expression profile of individual cells. Major advantage of this approach is an analysis of highly complex and heterogeneous cell populations, such as a specific subpopulation of T helper cells that are known to differentiate into distinct subpopulations. The need for distinguishing the specific expression profile is even more important considering the T cell plasticity. Read More

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Colchicine alleviates inflammation and improves diastolic dysfunction in heart failure rats with preserved ejection fraction.

Eur J Pharmacol 2022 Jun 29:175126. Epub 2022 Jun 29.

Department of Cardiology, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, 210008, PR China. Electronic address:

Purpose: Several studies have reported that colchicine attenuates cardiac inflammation and improves cardiac function in myocardial infarction and atrial fibrillation. However, no study has investigated its effect on heart failure with preserved ejection fraction (HFpEF). Hence, this study aimed to assess its efficacy in a high salt diet (HSD)-induced HFpEF rat model. Read More

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Spent LiFePO4: An old but vigorous peroxymonosulfate activator for degradation of organic pollutants in water.

Environ Res 2022 Jun 29:113780. Epub 2022 Jun 29.

Shanghai Collaborative Innovation Centre for WEEE Recycling, School of Resources and Environmental Engineering, Shanghai Polytechnic University, Shanghai, 201209, China. Electronic address:

Iron-based catalysts have been demonstrated to activate peroxymonosulfate (PMS) to generate reactive radicals, which is however limited by their complex preparation process, high costs and inefficiency for practical applications. Herein we obtain spent LiFePO (SLFP), with powerful catalytic capacity by a simple one-step treatment of the retired LiFePO cathode material, for PMS activation to decontaminate organic pollutants. Lithium defects and oxygen vacancies in SLFP play critical roles for PMS utilization, further confirmed by density functional theory (DFT) calculations. Read More

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Bioinformatics-based analysis reveals IDR-1018-mediated ceRNA regulation network for protective effect on hypoxia-ischemic brain injury in neonatal mice.

Exp Neurol 2022 Jun 29:114159. Epub 2022 Jun 29.

Department of Neonatology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, PR China.

Activation of an innate immune response serves as a key, contributing factor in perinatal brain injury. The current study sought to evaluate the clinical significance of innate defense regulatory peptide 1018 (IDR-1018)-derived peptide mediating ceRNA regulation network as a biomarker in neonatal mice with hypoxic-ischemic brain damage (HIBD). Firstly, bioinformatics analyses were performed to screen the HIBD-related candidate genes, miRNAs, and lncRNAs. Read More

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Podocyte-specific Nlrp3 inflammasome activation promotes diabetic kidney disease.

Kidney Int 2022 Jun 29. Epub 2022 Jun 29.

Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostic, University Hospital, Leipzig, 04103 Leipzig, Germany. Electronic address:

Efficient therapies for diabetic kidney disease (DKD), now the leading cause of kidney failure, are lacking. One hallmark of DKD is sterile inflammation (inflammation in absence of microorganisms), but the underlying molecular mechanisms remain poorly understood. The NLRP3 inflammasome (innate immune system receptors and sensors regulating activation of caspase-1) is a mechanism of sterile inflammation known to be activated by metabolic stimuli and reactive metabolites associated with DKD, including inflammasome activation in podocytes. Read More

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A rationale for the unlike potency of avibactam and ETX2514 against OXA-24 β-lactamase.

Arch Biochem Biophys 2022 Jun 29:109343. Epub 2022 Jun 29.

Department of Physical Chemistry, Universidad de Concepción, Chile; Millennium Nucleus on Catalytic Processes Towards Sustainable Chemistry, Santiago, 4070386, Chile. Electronic address:

Diazabicyclooctanone inhibitors such as ETX2514 and avibactam have shown enhanced inhibitory performance to fight the antibiotic resistance developed by pathogens. However, avibactam is ineffective against Acinetobacter baumannii infections, unlike ETX2514. The molecular basis for this difference has not been tackled from a molecular approach, precluding the knowledge of relevant information. Read More

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Deploying a Novel Custom Mobile Application for STEMI Activation and Transfer in a Large Healthcare System to Improve Cross-Team Workflow. STEMIcathAID Implementation Project.

Am Heart J 2022 Jun 29. Epub 2022 Jun 29.

The Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. Electronic address:

Background: ST-segment elevation myocardial infarction (STEMI) is a high-risk patient medical emergency. We developed a secure mobile application, STEMIcathAID, to optimize care for STEMI patients by providing a digital platform for communication between the STEMI care team members, EKG transmission, cardiac catherization laboratory (cath lab) activation and ambulance tracking. The aim of this report is to describe the implementation of the app into the current STEMI workflow in preparation for a pilot project employing the app for inter-hospital STEMI transfer. Read More

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Visible-light-assisted persulfate activation by SnS/MIL-88B(Fe) Z-scheme heterojunction for enhanced degradation of ibuprofen.

J Colloid Interface Sci 2022 Jun 26;625:965-977. Epub 2022 Jun 26.

Key Laboratory of Organic Compound Pollution Control Engineering (MOE), Shanghai University, Shanghai 200444, PR China; School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, PR China. Electronic address:

Herein, a highly efficient Z-scheme SnS/MIL-88B (Fe) (SnSFe) heterojunction was successfully synthesized to use both as photocatalysts and persulfate (PS) activator for ibuprofen (IBP) degradation. Flower-liked SnS was uniformly loaded on MIL-88B (Fe), and SnSFe retained the original polyhedral morphology of MIL-88B (Fe). The highest removal of IBP was achieved in the presence of SnSFe with 0. Read More

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Crosstalk between macrophages and innate lymphoid cells (ILCs) in diseases.

Int Immunopharmacol 2022 Jun 29;110:108937. Epub 2022 Jun 29.

Central Laboratory, The first affiliated hospital of Wannan Medical College, Wuhu, PR China; Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institutes (Wannan Medical College), Wuhu, PR China; Anhui Province Clinical Research Center for Critical Respiratory Medicine. Electronic address:

Innate lymphoid cells (ILCs) and macrophages are tissue-resident cells that play important roles in tissue-immune homeostasis and immune regulation. ILCs are mainly distributed on the barrier surfaces of mammals to ensure immunity or tissue homeostasis following host, microbial, or environmental stimulation. Their complex relationships with different organs enable them to respond quickly to disturbances in environmental conditions and organ homeostasis, such as during infections and tissue damage. Read More

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The m6A reader YTHDF3-mediated PRDX3 translation alleviates liver fibrosis.

Redox Biol 2022 Jun 24;54:102378. Epub 2022 Jun 24.

Department of Pharmacology, Dalian Medical University, Dalian, China. Electronic address:

Peroxiredoxin 3 (PRDX3) acts as a master regulator of mitochondrial oxidative stress and exerts hepatoprotective effects, but the role of PRDX3 in liver fibrosis is not well understood. N-methyladenosine (m6A) is considered the most prevalent posttranscriptional modification of mRNA. This study aimed to elucidate the effect of PRDX3 on liver fibrosis and the potential mechanism through which the m6A modification regulates PRDX3. Read More

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8-Oxypalmatine, a novel oxidative metabolite of palmatine, exhibits superior anti-colitis effect via regulating Nrf2 and NLRP3 inflammasome.

Biomed Pharmacother 2022 Jun 29;153:113335. Epub 2022 Jun 29.

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China; Dongguan Institute of Guangzhou University of Chinese Medicine, Dongguan 523808, PR China. Electronic address:

Palmatine (PAL) is an isoquinoline alkaloid derived from Fibraureae caulis Pierre that has been used to relieve inflammatory diseases like ulcerative colitis (UC). The metabolites of PAL were believed to contribute significantly to its outstanding biological activities. 8-Oxypalmatine (OPAL), a liver-mediated oxidative metabolite of PAL, has been firstly identified in the present work. Read More

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The influence of Nigella sativa essential oil on proliferation, activation, and apoptosis of human T lymphocytes in vitro.

Biomed Pharmacother 2022 Jun 29;153:113349. Epub 2022 Jun 29.

Department of Physiopathology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland. Electronic address:

In previous work, we tested the immunomodulatory effect of Nigella sativa (NS) fatty oil. Our results demonstrated that unrefined, obtained by cold pressing black cumin seed oil inhibited lymphocytes' proliferation and induced their apoptosis in a dose-dependent manner. In this study, we examined the immunomodulatory properties of essential oil (EO) obtained from the NS seeds by hydrodistillation and its two main constituents: thymoquinone (TQ) and p-cymene. Read More

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Deciphering combinatorial post-translational modifications by top-down mass spectrometry.

Curr Opin Chem Biol 2022 Jun 29;70:102180. Epub 2022 Jun 29.

Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA. Electronic address:

Post-translational modifications (PTMs) create vast structural and functional diversity of proteins, ultimately modulating protein function and degradation, influencing cellular signaling, and regulating transcription. The combinatorial patterns of PTMs increase the heterogeneity of proteins and further mediates their interactions. Advances in mass spectrometry-based proteomics have resulted in identification of thousands of proteins and allowed characterization of numerous types and sites of PTMs. Read More

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The interplay between parental care and OPRM1 in reward responsiveness.

Psychoneuroendocrinology 2022 Jun 21;143:105845. Epub 2022 Jun 21.

College of Life Science, Northwest University, Xi'an 710069, China; College of Medicine, Northwest University, Xi'an 710069, China; Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an 710069, China; Institute of Population and Health, Northwest University, Xi'an 710069, China; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi'an 710069, China. Electronic address:

The Reinforcement Sensitivity Theory (RST) explains a variety of reward-motivated behaviors as the result of the activation of biologically-based systems. Inspired by the influences of parental bonding and opioid peptide on reward system, we investigated the contributions of parental bonding and mu-opioid receptor gene (OPRM1) towards motivation systems (i.e. Read More

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Methyl butyrate attenuates concanavalin A-induced autoimmune hepatitis by inhibiting Th1-cell activation and homing to the liver.

Cell Immunol 2022 Jun 24;378:104575. Epub 2022 Jun 24.

Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, 200040, China. Electronic address:

Con A-induced hepatitis is the most commonly used animal model for simulating autoimmune hepatitis (AIH). In this study, we investigated whether methyl butyrate (MB) alleviates Con A-induced hepatitis and how it affects Con A-stimulated lymphocytes. MB improves liver function in AIH mice, reducing the expression of several inflammatory cytokines and Th1 cell-associated chemokines in the liver, while significantly inhibiting toll-like receptor signaling pathway. Read More

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