29,625 results match your criteria activating transcription


FoxO1 in Micropterus salmoides: Molecular characterization and its roles in glucose metabolism by glucose or insulin-glucose loading.

Gen Comp Endocrinol 2021 May 9:113811. Epub 2021 May 9.

National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address:

Forkhead box O1 (FoxO1), a nuclear transcription factor, plays an important role in insulin-mediated glucose metabolism. In this study, FoxO1 gene from largemouth bass (Micropterus salmoides) was cloned and characterized, and its effects on hepatic glucose metabolism regulated by insulin-AKT pathway were investigated in response to glucose or insulin-glucose injection. The full-length cDNA of FoxO1 consisted of 2541 bp and encoded 680 amino acids. Read More

View Article and Full-Text PDF

Activating transcription factor 4-dependent lactate dehydrogenase activation as a protective response to amyloid beta toxicity.

Brain Commun 2021 26;3(2):fcab053. Epub 2021 Mar 26.

Department of Genetics, Evolution and Environment, Institute of Healthy Ageing, University College London, London WC1E 6BT, UK.

Accumulation of amyloid beta peptides is thought to initiate the pathogenesis of Alzheimer's disease. However, the precise mechanisms mediating their neurotoxicity are unclear. Our microarray analyses show that, in models of amyloid beta 42 toxicity, genes involved in the unfolded protein response and metabolic processes are upregulated in brain. Read More

View Article and Full-Text PDF

LncRNA NCK1-AS1 exerts oncogenic property in gastric cancer by targeting the miR-22-3p/BCL9 axis to activate the Wnt/β-catenin signaling.

Environ Toxicol 2021 May 11. Epub 2021 May 11.

Department of Gastroenterology, Jinhu People's Hospital, Huaian, Jiangsu, China.

Long noncoding RNAs (lncRNAs) exert crucial effects on the development of many malignancies, including gastric cancer. Herein, we investigated the role of lncRNA noncatalytic region of tyrosine kinase adaptor protein 1 (NCK1) divergent transcript (NCK1-DT, also known as NCK1-AS1) in gastric cancer. Reverse transcription quantitative polymerase chain reaction demonstrated that NCK1-AS1 exhibited high expression in gastric cancer tissues and cells. Read More

View Article and Full-Text PDF

A comparative study of toxicity of graphdiyne and graphene oxide to human umbilical vein endothelial cells.

J Appl Toxicol 2021 May 10. Epub 2021 May 10.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

The success of graphene oxide (GO) has attracted extensive research interests in developing novel 2D nanomaterials (NMs). Graphdiyne (GDY) is a new member of carbon-based 2D NMs possessing sp- and sp -hybridized carbon atoms. However, the toxicity of GDY is less investigated as GO. Read More

View Article and Full-Text PDF

Spermidine inhibits vascular calcification in chronic kidney disease through modulation of SIRT1 signaling pathway.

Aging Cell 2021 May 9:e13377. Epub 2021 May 9.

Department of Cardiology, Laboratory of Heart Center, Heart Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Vascular calcification is a common pathologic condition in patients with chronic kidney disease (CKD) and aging individuals. It has been established that vascular calcification is a gene-regulated biological process resembling osteogenesis involving osteogenic differentiation. However, there is no efficient treatment available for vascular calcification so far. Read More

View Article and Full-Text PDF

The Cell Culture Environment Regulates the Transcription Factor MafB in BV-2 Microglia.

Matters (Zur) 2021 29;2021. Epub 2021 Jan 29.

Center for Neurotherapeutics Discovery, Department of Neuroscience, Department of Immunology and Microbiology, Department of Neurology, Department of Pediatrics, University of Rochester Medical Center.

Microglia experience dramatic molecular and functional changes when transferred from the central nervous system (CNS) to a cell culture environment. Investigators largely attribute these findings to the loss of CNS-specific microenvironmental cues that dictate the gene-regulatory networks specified by master regulator transcription factors such as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B (MafB). MafB regulates macrophage differentiation and activation by activating or repressing target genes critical to these processes. Read More

View Article and Full-Text PDF
January 2021

Pathological β-Cell Endoplasmic Reticulum Stress in Type 2 Diabetes: Current Evidence.

Front Endocrinol (Lausanne) 2021 22;12:650158. Epub 2021 Apr 22.

ULB Center for Diabetes Research, Medical Faculty, Université Libre de Bruxelles, Brussels, Belgium.

The notion that in diabetes pancreatic β-cells express endoplasmic reticulum (ER) stress markers indicative of increased unfolded protein response (UPR) signaling is no longer in doubt. However, what remains controversial is whether this increase in ER stress response actually contributes importantly to the β-cell failure of type 2 diabetes (akin to 'terminal UPR'), or whether it represents a coping mechanism that represents the best attempt of β-cells to adapt to changes in metabolic demands as presented by disease progression. Here an intercontinental group of experts review evidence for the role of ER stress in monogenic and type 2 diabetes in an attempt to reconcile these disparate views. Read More

View Article and Full-Text PDF

Protective effect and mechanisms of action of Mongolian medicine Sulongga-4 on pyloric ligation-induced gastroduodenal ulcer in rats.

World J Gastroenterol 2021 Apr;27(16):1770-1784

School of Mongolian Medicine, Inner Mongolia University for Nationalities, Tongliao 028000, Inner Mongolia Autonomous Region, China.

Background: Sulongga-4 (SL-4) is a herbal formula used in traditional Mongolian medical clinics for the treatment of peptic ulcers and gastroenteritis, even though its pharmacological mechanism has not been well characterized.

Aim: To evaluate the protective effect and identify the mechanisms of action of SL-4 on gastroduodenal ulcer induced by pyloric ligation (PL) in rats.

Methods: PL was performed to induce gastric and duodenal ulcers in rats, which were then treated with oral SL-4 (1. Read More

View Article and Full-Text PDF

Dual role of miR-1 in the development and function of sinoatrial cells.

J Mol Cell Cardiol 2021 May 5. Epub 2021 May 5.

Department of Biosciences, Università degli Studi di Milano, Milan, Italy. Electronic address:

miR-1, the most abundant miRNA in the heart, modulates expression of several transcription factors and ion channels. Conditions affecting the heart rate, such as endurance training and cardiac diseases, show a concomitant miR-1 up- or down-regulation. Here, we investigated the role of miR-1 overexpression in the development and function of sinoatrial (SAN) cells using murine embryonic stem cells (mESC). Read More

View Article and Full-Text PDF

GCN4 regulates secondary metabolism through activating antioxidant genes expression under nitrogen limitation in .

Appl Environ Microbiol 2021 May 7. Epub 2021 May 7.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture; Microbiology Department, College of Life Sciences, Agricultural University, Jiangsu, Nanjing 210095, People's Republic of China.

Nitrogen limitation has been widely reported to affect the growth and development of fungi, and the transcription factor GCN4 (general control nonderepressible 4) is involved in nitrogen restriction. Here, we found that nitrogen limitation highly induced the expression of GCN4 and promoted the synthesis of ganoderic acid (GA), an important secondary metabolite in The activated GCN4 is involved in regulating GA biosynthesis. Besides, the accumulation of ROS is also affecting the synthesis of GA under nitrogen restrictions. Read More

View Article and Full-Text PDF

The Ca -binding protein sorcin stimulates transcriptional activity of the unfolded protein response mediator ATF6.

FEBS Lett 2021 May 7. Epub 2021 May 7.

Section of Cell Biology and Functional Genomics, Department of Metabolism, Digestion & Reproduction, Imperial College London, UK.

Sorcin is a calcium-binding protein involved in maintaining endoplasmic reticulum (ER) Ca stores. We have previously shown that overexpressing sorcin under the rat insulin promoter was protective against high fat diet-induced pancreatic beta cell dysfunction in vivo. Activating Transcription Factor 6 (ATF6) is a key mediator of the unfolded protein response (UPR) that provides cellular protection during the progression of ER stress. Read More

View Article and Full-Text PDF

Influence of genetic variants in asparaginase pathway on the susceptibility to asparaginase-related toxicity and patients' outcome in childhood acute lymphoblastic leukemia.

Cancer Chemother Pharmacol 2021 May 7. Epub 2021 May 7.

Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.

Background: Asparaginase (ASNase) is a key component in the treatment protocols of childhood acute lymphoblastic leukemia (ALL). Asparagine synthetase (ASNS) and the basic region leucine zipper activating transcription factor 5 (ATF5) mediate the anti-leukemic effect of ASNase. Only a few reports studied the association between polymorphisms in these genes and treatment-related toxicity and response. Read More

View Article and Full-Text PDF

Exportin-4 coordinates nuclear shuttling of TOPLESS family transcription corepressors to regulate plant immunity.

Plant Cell 2021 May;33(3):697-713

Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.

The regulated nucleocytoplasmic exchange of macromolecules is essential for the eukaryotic cell. However, nuclear transport pathways defined by different nuclear transport receptors (NTRs), including importins and exportins, and their significance in activating distinct stress responses are poorly understood in plants. Here, we exploited a CRISPR/Cas9-based genetic screen to search for modifiers of CONSTITUTIVE EXPRESSION OF PATHOGENESIS-RELATED GENE 5 (cpr5), an Arabidopsis thaliana nucleoporin mutant that activates autoimmune responses that partially mimic effector-triggered immunity (ETI). Read More

View Article and Full-Text PDF

Mitochondrial Disruption Is Involved in the Effect of Nuclear Factor of Activated T cells, Cytoplasmic 4 on Aggravating Cardiomyocyte Hypertrophy.

J Cardiovasc Pharmacol 2021 May;77(5):557-569

Department of Pharmacy, School of Medicine, Guangxi University of Science and Technology, Liuzhou, Guangxi, People's Republic of China; and.

Abstract: Nuclear factor of activated T cells, cytoplasmic 4 (NFATc4), a nuclear transcription factor, has been implicated in cardiac hypertrophy through the enhancement of hypertrophic gene expression. However, the role of NFATc4 in mitochondrial modulation is mostly unknown. The current study aimed to investigate the role of NFATc4 in regulating mitochondrial function during phenylephrine (PE)-induced cardiac hypertrophy. Read More

View Article and Full-Text PDF

Two bHLH transcription factors, bHLH48 and bHLH60, associate with phytochrome interacting factor 7 to regulate hypocotyl elongation in Arabidopsis.

Cell Rep 2021 May;35(5):109054

State Key Laboratory of Genetic Engineering and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, People's Republic of China. Electronic address:

The basic helix-loop-helix (bHLH) transcription factor PHYTOCHROME-INTERACTING FACTOR 7 (PIF7) is a central regulator that promotes stem growth by activating growth-related gene expression during shade-avoidance responses. Studying the co-factors of PIF7 can facilitate understanding of the mechanism of PIFs and light signal transduction. Here, we describe the identification of two bHLH transcription factors, bHLH48 and bHLH60 (bHLH48/bHLH60), as essential partners for PIF7-dependent modulation of hypocotyl elongation and function downstream of phytochrome B. Read More

View Article and Full-Text PDF

Cooperation of ATF4 and CTCF promotes adipogenesis through transcriptional regulation.

Cell Biol Toxicol 2021 May 5. Epub 2021 May 5.

Guangdong Provincial Key Laboratory of Biomedical Imaging and Guangdong Provincial Engineering Research Center of Molecular Imaging, the Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong Province, 519000, People's Republic of China.

Adipogenesis is a multi-step process orchestrated by activation of numerous TFs, whose cooperation and regulatory network remain elusive. Activating transcription factor 4 (ATF4) is critical for adipogenesis, yet its regulatory network is unclarified. Here, we mapped genome-wide ATF4 binding landscape and its regulatory network by Chip-seq and RNA-seq and found ATF4 directly modulated transcription of genes enriching in fat cell differentiation. Read More

View Article and Full-Text PDF

LncRNA AFAP1-AS1 regulates proliferation and apoptosis of endometriosis through activating STAT3/TGF-β/Smad signaling via miR-424-5p.

J Obstet Gynaecol Res 2021 May 4. Epub 2021 May 4.

Reproductive Center, The First People's Hospital of Yueyang, Yueyang, China.

Aim: Endometriosis is a common gynecological disorder characterized by chronic pelvic pain and infertility, which negatively affects women's health worldwide. AFAP1-AS1 has been implicated in endometriosis lesions recently, but its mechanism of endometriosis progression remains unclear.

Methods: Endometrial stromal cells (ESCs) were used to identify the role of AFAP1-AS1 in endometriosis. Read More

View Article and Full-Text PDF

Activating transcription factor 4 regulates angiogenesis under lipid overload via methionine adenosyltransferase 2A-mediated endothelial epigenetic alteration.

FASEB J 2021 Jun;35(6):e21612

Department of Cardiology, West China Hospital, Sichuan University, Chengdu, P.R. China.

Lipid overload is intimately connected with the change of endothelial epigenetic status which impacts cellular signaling activities and endothelial function. Activating transcription factor 4 (ATF4) is involved in the regulation of lipid metabolism and meanwhile an epigenetic modifier. However, the role of ATF4 in the angiogenesis under lipid overload is not well understood. Read More

View Article and Full-Text PDF

Tumor necrosis factor-induced ArhGEF10 selectively activates RhoB contributing to human microvascular endothelial cell tight junction disruption.

FASEB J 2021 Jun;35(6):e21627

Department of Pediatrics, Yale School of Medicine, Yale University, New Haven, CT, USA.

Capillary endothelial cells (ECs) maintain a semi-permeable barrier between the blood and tissue by forming inter-EC tight junctions (TJs), regulating selective transport of fluid and solutes. Overwhelming inflammation, as occurs in sepsis, disrupts these TJs, leading to leakage of fluid, proteins, and small molecules into the tissues. Mechanistically, disruption of capillary barrier function is mediated by small Rho-GTPases, such as RhoA, -B, and -C, which are activated by guanine nucleotide exchange factors (GEFs) and disrupted by GTPase-activating factors (GAPs). Read More

View Article and Full-Text PDF

Periplocin and cardiac glycosides suppress the unfolded protein response.

Sci Rep 2021 May 4;11(1):9528. Epub 2021 May 4.

Department of Cell Signaling, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan.

The unfolded protein response (UPR) controls protein homeostasis through transcriptional and translational regulation. However, dysregulated UPR signaling has been associated with the pathogenesis of many human diseases. Therefore, the compounds modulating UPR may provide molecular insights for these pathologies in the context of UPR. Read More

View Article and Full-Text PDF

OPA1 deletion in brown adipose tissue Improves thermoregulation and systemic metabolism via FGF21.

Elife 2021 May 4;10. Epub 2021 May 4.

Fraternal Order of Eagles Diabetes Research Center and Division of Endocrinology and Metabolism, University of Iowa, Iowa City, United States.

Adrenergic stimulation of brown adipocytes alters mitochondrial dynamics, including the mitochondrial fusion protein optic atrophy 1 (OPA1). However, direct mechanisms linking OPA1 to brown adipose tissue (BAT) physiology are incompletely understood. We utilized a mouse model of selective OPA1 deletion in BAT (OPA1 BAT KO) to investigate the role of OPA1 in thermogenesis. Read More

View Article and Full-Text PDF

The histone reader PHF7 cooperates with the SWI/SNF complex at cardiac super enhancers to promote direct reprogramming.

Nat Cell Biol 2021 May 3. Epub 2021 May 3.

Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Direct cardiac reprogramming of fibroblasts to cardiomyocytes presents an attractive therapeutic strategy to restore cardiac function following injury. Cardiac reprogramming was initially achieved through overexpression of the transcription factors Gata4, Mef2c and Tbx5; later, Hand2 and Akt1 were found to further enhance this process. Yet, staunch epigenetic barriers severely limit the ability of these cocktails to reprogramme adult fibroblasts. Read More

View Article and Full-Text PDF

Sympathectomy decreases pain behaviors and nerve regeneration by downregulating monocyte chemokine CCL2 in dorsal root ganglia in the rat tibial nerve crush model.

Pain 2021 Apr 30. Epub 2021 Apr 30.

Pain Research Center, Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, U.S.A. Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China. *Present address.

Abstract: Peripheral nerve regeneration is associated with pain in several preclinical models of neuropathic pain. Some neuropathic pain conditions and preclinical neuropathic pain behaviors are improved by sympathetic blockade. In this study we examined the effect of a localized "microsympathectomy," i. Read More

View Article and Full-Text PDF

Ume6 acts as a stable platform to coordinate repression and activation of early meiosis-specific genes in .

Mol Cell Biol 2021 May 3. Epub 2021 May 3.

Department of Biochemistry, 561 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada, 2H7 L2A

In response to nutrient starvation the budding yeast abandons mitotic proliferation and embarks on a differentiation process leading through meiosis to the formation of haploid spores. This process is driven by cascading waves of meiosis-specific gene expression. The early meiosis-specific genes are repressed during mitotic proliferation by DNA-binding protein Ume6 in combination with repressors Rpd3 and Sin3. Read More

View Article and Full-Text PDF

LncRNA MIR31HG is activated by STAT1 and facilitates glioblastoma cell growth via Wnt/β-catenin signaling pathway.

Neurosci Res 2021 Apr 30. Epub 2021 Apr 30.

Neurosurgery Department, Marina Bay Central Hospital, Dongguan, 523899, Guangdong, China. Electronic address:

Long non-coding RNAs (lncRNAs) have been reported to biologically regulate tumor progression. LncRNA MIR31HG has been identified as an oncogene in several cancer types, but its role and mechanism in glioblastoma (GBM) remain to be explored. In the present study, we detected strongly-expressed MIR31HG in GBM cells through RT-qPCR analysis. Read More

View Article and Full-Text PDF

miR-204-5p and miR-211 Synergistically Downregulate the α-Casein Content and Contribute to the Lower Allergy of Goat Milk.

J Agric Food Chem 2021 May 3;69(18):5353-5362. Epub 2021 May 3.

Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, Shaanxi 712100, P. R. China.

α-Casein (encoded by the 11 gene) is associated with higher rates of allergy than other milk protein components for humans. microRNAs (miRNAs) as small noncoding RNA molecules regulate gene expression and influence diverse biological processes. However, little is known about the regulation of milk protein synthesis by miRNAs in ruminants. Read More

View Article and Full-Text PDF

Enhancing Fatty Acid Catabolism of Macrophages Within Aberrant Breast Cancer Tumor Microenvironment Can Re-establish Antitumor Function.

Front Cell Dev Biol 2021 15;9:665869. Epub 2021 Apr 15.

Institute of Cancer Prevention and Treatment, Harbin Medical University, Harbin, China.

Triple-negative breast cancer (TNBC) remains an intractable challenge owing to its aggressive nature and lack of any known therapeutic targets. Macrophages play a crucial role in cancer promotion and poor prognosis within the tumor microenvironment (TME). The phagocytosis checkpoint in macrophages has broader implications for current cancer immunotherapeutic strategies. Read More

View Article and Full-Text PDF

Identification of UL69 Gene and Protein in Cytomegalovirus-Transformed Human Mammary Epithelial Cells.

Front Oncol 2021 16;11:627866. Epub 2021 Apr 16.

Department Pathogens & Inflammation-EPILAB EA4266, University of Bourgogne France-Comté, Besançon, France.

A growing body of evidence addressing the involvement of human cytomegalovirus (HCMV) in malignancies had directed attention to the oncomodulation paradigm. HCMV-DB infected human mammary epithelial cells (HMECs) in culture showed the emergence of clusters of rapidly proliferating, spheroid-shaped transformed cells named CTH (CMV-Transformed HMECs) cells. CTH cells assessment suggests a direct contribution of HCMV to oncogenesis, from key latent and lytic genes activating oncogenic pathways to fueling tumor evolution. Read More

View Article and Full-Text PDF

RUNX1 regulates SMAD1 by transcriptionally activating the expression of USP9X, regulating the activation of hepatic stellate cells and liver fibrosis.

Eur J Pharmacol 2021 Apr 30;903:174137. Epub 2021 Apr 30.

Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming City, Yunan Province, 650101, China.

Liver fibrosis (LF) is a common pathological process with high morbidity and mortality. Runt-related transcription factor 1 (RUNX1) is a transcription factor that could cause nephropathy and renal fibrosis, but its role in LF is unclear. Therefore, this study aimed to investigate the role RUNX1 in LF. Read More

View Article and Full-Text PDF

The Cdk8 kinase module regulates interaction of the Mediator complex with RNA polymerase II.

J Biol Chem 2021 Apr 29:100734. Epub 2021 Apr 29.

Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. Electronic address:

The Cdk8 kinase module (CKM) is a dissociable part of the coactivator complex Mediator, which regulates gene transcription by RNA polymerase II (Pol II). The CKM has both negative and positive functions in gene transcription that remain poorly understood at the mechanistic level. In order to reconstitute the role of the CKM in transcription initiation, we prepared recombinant CKM from the yeast Saccharomyces cerevisiae. Read More

View Article and Full-Text PDF