9,595 results match your criteria acids isolate

Characterization of Iturin V, a Novel Antimicrobial Lipopeptide from a Potential Probiotic Strain Lactobacillus sp. M31.

Probiotics Antimicrob Proteins 2021 May 13. Epub 2021 May 13.

CSIR-Institute of Microbial Technology, Chandigarh, India.

Members of lactic acid bacteria group are known to produce various antimicrobial substances. Cyclic lipopeptides are one such potent class of amphipathic natural biosurfactants that exhibit bactericidal and immunomodulatory properties. In this study, we aimed to investigate antimicrobial and immunomodulatory activities of a lipopeptide secreted by a LAB isolate strain M31 identified as a member of the genus Lactobacillus. Read More

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Anaerococcus faecalis sp. nov., Isolated from Swine Faeces.

Curr Microbiol 2021 May 13. Epub 2021 May 13.

Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea.

An obligate anaerobic, Gram-stain-positive, non-spore forming, non-motile, catalase and oxidase-negative, coccoid-shaped bacterium designated AGMB00486 was isolated from swine faeces. The optimal growth of the isolate occurred at pH 8.0 and 37 ℃. Read More

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Tryptophan Operon Diversity Reveals Evolutionary Trends among Geographically Disparate Chlamydia trachomatis Ocular and Urogenital Strains Affecting Tryptophan Repressor and Synthase Function.

mBio 2021 May 11;12(3). Epub 2021 May 11.

Department of Pediatrics, University of California San Francisco, Oakland, California, USA

The obligate intracellular pathogen () is the leading cause of bacterial sexually transmitted infections and blindness globally. To date, urogenital strains are considered tryptophan prototrophs, utilizing indole for tryptophan synthesis within a closed-conformation tetramer comprised of two α (TrpA)- and two β (TrpB)-subunits. In contrast, ocular strains are auxotrophs due to mutations in TrpA, relying on host tryptophan pools for survival. Read More

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gen. nov., sp. nov., a novel glycoside hydrolase positive bacterial strain with ginsenoside hydrolysing activity.

Int J Syst Evol Microbiol 2021 May;71(5)

AceEMzyme Co., Ltd., Academic Industry Cooperation, 327 Jungang-ro Anseong-si, Gyeonggi-do 17579, Republic of Korea.

The conversion of major ginsenosides into minor ginsenosides attracts a lot of interest because of their biological and pharmaceutical activities. Therefore, for the conversion of ginsenosides, finding a novel competent glycoside hydrolase-producing bacterial strain is useful for future research studies and the mass production of minor ginsenosides. Wastewater samples were collected and screened for novel glycoside hydrolase bacterial strains using Reasoner's 2A+aesculin agar medium. Read More

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Planococcus soli sp. nov., isolated from antarctic soil.

Antonie Van Leeuwenhoek 2021 May 11. Epub 2021 May 11.

Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, 100081, Beijing, People's Republic of China.

A novel bacterial strain, designated C23, was isolated from a soil sample obtained from King George Island, Antarctica. Phenotypic, phylogenetic, chemotaxonomic and molecular analyses were performed on the new isolate. Strain C23 formed orange colonies on agar plates and was Gram-stain-positive. Read More

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Molecular and biological characterization of Potato virus Y detected in zucchini in China.

Virusdisease 2021 Mar 18;32(1):117-122. Epub 2021 Mar 18.

College of Hortculture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, 010019 Inner Mongolia China.

Potato virus Y (PVY) is an important viral pathogen of the plant family . However, it has not been reported that PVY infects zucchini ( L). Zucchini plants showing symptoms of leaf mosaic and fruit ringspot were collected from Inner Mongolia, China. Read More

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Cultivation of Nostoc sp. LS04 in municipal wastewater for biodiesel production and their deoiled biomass cellular extracts as biostimulants for Lactuca sativa growth improvement.

Chemosphere 2021 Apr 26;280:130644. Epub 2021 Apr 26.

Centro de Investigación en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile; Scientific and Technological Bioresource Nucleus, BIOREN-UFRO, Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile. Electronic address:

In this study, seven different cyanobacteria (LS01-LS07) were isolated from paddy field water and among them, the isolate LS04 was able to grow well on municipal wastewater. The LS04 isolate was identified as Nostoc sp. (designated as Nostoc sp. Read More

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sp. nov., a new member of family isolated from sand fly rearing substrate.

Int J Syst Evol Microbiol 2021 May;71(5)

Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, USA.

A Gram-stain-negative, rod-shaped, non-motile, non-spore-forming, aerobic bacterium, designated type strain SSI9, was isolated from sand fly ( Scopoli; ) rearing substrate and subjected to polyphasic taxonomic analysis. Strain SSI9 contained phosphatidylethanolamine as a major polar lipid, MK-7 as the predominant quinone, and Cω6/Cω, iso-C, iso-C 3-OH and C as the major cellular fatty acids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that SSI9 represents a member of the genus , of the family Sphingobacteriaceae sharing 96. Read More

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Phenotypic and genomic characterization of a strain causing disease in provides insights into its niche adaptation and pathogenic mechanism.

Microb Genom 2021 May;7(5)

Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin 300384, PR China.

The virulence of is variable depending on its virulence determinants. A strain, in which the virulence is governed by the and genes, can cause acute hepatopancreatic necrosis disease (AHPND) in shrimps. Some that are non-AHPND strains also cause shrimp diseases and result in huge economic losses, while their pathogenicity and pathogenesis remain unclear. Read More

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Direct identification and molecular characterization of zoonotic hazards in raw milk by metagenomics using as a model pathogen.

Microb Genom 2021 May;7(5)

Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.

Metagenomics is a valuable diagnostic tool for enhancing microbial food safety because (i) it enables the untargeted detection of pathogens, (ii) it is fast since primary isolation of micro-organisms is not required, and (iii) it has high discriminatory power allowing for a detailed molecular characterization of pathogens. For shotgun metagenomics, total nucleic acids (NAs) are isolated from complex samples such as foodstuff. Along with microbial NAs, high amounts of matrix NAs are extracted that might outcompete microbial NAs during next-generation sequencing and compromise sensitivity for the detection of low abundance micro-organisms. Read More

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Impact of arabinoxylan on characteristics, stability and lipid oxidation of oil-in-water emulsions: Arabinoxylan from wheat bran, corn bran, rice bran, and rye bran.

Food Chem 2021 Apr 20;358:129813. Epub 2021 Apr 20.

College of Food Science, Sichuan Agricultural University, No.46 Xinkang Road, Yucheng District, Yaan, Sichuan 625014, China. Electronic address:

To investigate the impact of arabinoxylan (AX) on the physical and oxidative stability of oil-in-water emulsions, AX from wheat bran, corn bran, rice bran, or rye bran was incorporated into the production of whey protein isolate-stabilised emulsions. Decreased interfacial charge and increased mean particle diameters were recorded in all fresh emulsions with 0.1%-0. Read More

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First report of Tomato black ring virus infecting raspberry and blackberry in Poland.

Plant Dis 2021 May 2. Epub 2021 May 2.

Warsaw University of Life Sciences, 49561, Plant Protection, Section of Phytopathology, Warsaw, Poland;

Raspberry ( L.) and blackberry ( L.) are infected by at least 29 viruses, including the Tomato black ring virus (TBRV) (Martin et al. Read More

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Proteomic Charting of Imipenem Adaptive Responses in a Highly Carbapenem Resistant Clinical Isolate.

Antibiotics (Basel) 2021 Apr 28;10(5). Epub 2021 Apr 28.

University Medical Center Groningen, Department of Medical Microbiology and Infection Prevention, University of Groningen, Hanzeplein 1, P.O. Box 30001, 9700 RB Groningen, The Netherlands.

Gram-negative bacteria belonging to the complex are increasingly implicated in difficult-to-treat nosocomial infections, as exemplified by a recently characterized highly carbapenem-resistant clinical isolate with sequence type (ST) 232. While mechanisms of carbapenem resistance are well-understood, little is known about the responses of highly drug-resistant bacteria to these antibiotics. Our present study was therefore aimed at charting the responses of the ST232 isolate to the carbapenem imipenem, using a 'stable isotope labeling of amino acids in cell culture' approach for quantitative mass spectrometry. Read More

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High-Quality Nucleic Acid Isolation from Hard-to-Lyse Bacterial Strains Using PMAP-36, a Broad-Spectrum Antimicrobial Peptide.

Int J Mol Sci 2021 Apr 16;22(8). Epub 2021 Apr 16.

Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Korea.

The efficiency of existing cell lysis methods to isolate nucleic acids from diverse bacteria varies depending on cell wall structures. This study tested a novel idea of using broad-spectrum antimicrobial peptides to improve the lytic efficiency of hard-to-lyse bacteria and characterized their differences. The lysis conditions of using recombinant porcine myeloid antimicrobial peptide 36 (PMAP-36), a broad-spectrum pig cathelicidin, was optimized, and RNA isolation was performed with cultured pellets of ten bacterial species using various membranolytic proteins. Read More

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Biomimetic, Smart, and Multivalent Ligands for G-Quadruplex Isolation and Bioorthogonal Imaging.

ACS Chem Biol 2021 Apr 29. Epub 2021 Apr 29.

ICMUB, CNRS UMR6302, UBFC Dijon, 9, Avenue Alain Savary, 21078 Dijon, France.

G-quadruplexes (G4s) continue to gather wide attention in the field of chemical biology as their prevalence in the human genome and transcriptome strongly suggests that they play key regulatory roles in cell biology. G4-specific, cell-permeable small molecules (G4-ligands) innovatively permit the interrogation of cellular circuitries in order to assess to what extent G4s influence cell fate and functions. Here, we report on multivalent, biomimetic G4-ligands referred to as TASQs that enable both the isolation and visualization of G4s in human cells. Read More

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Mucilaginibacter segetis sp. nov., Isolated from Soil.

Curr Microbiol 2021 Apr 28. Epub 2021 Apr 28.

School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.

A bacterial strain, SD-g, was isolated from a soil sample collected on Dokdo Island, South Korea. Cells were observed to be Gram stain negative and short rod shaped, and colonies to be pink in color. Growth of the isolate was observed at 4-30 °C, pH 6-8, and in the presence of 0-2. Read More

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First report of Boeremia exigua var. exigua causing branch blight on walnut in China.

Plant Dis 2021 Apr 28. Epub 2021 Apr 28.

Gansu Agricultural University, College of Plant Protection, No. 1 Yingmen Village, Anning District,, Lanzhou, Gansu, China, 730070;

Walnut (Juglans sinensis L.) is an important economic tree. Its fruit are rich in omega-3 essential fatty acids, which are valuable nutritionally (Cheon et al, 2013). Read More

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First Report of and Causing Canker Disease of Sacha Inchi in Hainan, China.

Plant Dis 2021 Apr 27. Epub 2021 Apr 27.

Hainan University, 74629, Hainan University, Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants (Hainan University), Ministry of Education /Key Laboratory of Germplasm Resources of Tropical Special Ornamental Plants of Hainan Province, College of Forest , Haikou 570228, Hainan, China;

Sacha inchi ( L.) belongs to the family Euphorbiaceae. It is a perennial wooden oilseed crop, and also exhibits a good source of polyunsaturated fatty acids, protein and other bioactive compounds, such as tocopherols, carotenes and phytosterols (Chirinos et al. Read More

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Methylobacterium radiodurans sp. nov., a novel radiation-resistant Methylobacterium.

Arch Microbiol 2021 Apr 24. Epub 2021 Apr 24.

Division of Applied Food System, Major in Food Science and Technology, Seoul Women's University, Seoul, 01797, Korea.

A Gram-negative, aerobic, flagellated, rod-shaped, and pink-pigmented bacterium, strain 17Sr1-43, was isolated from a soil sample collected in Nowongu, Seoul, Korea. The isolate could grow at 18-37 °C (optimum, 28-30 °C), pH 6.0-8. Read More

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Integrating metagenetics and high-throughput screening for bioprospecting marine thraustochytrids producers of long-chain polyunsaturated fatty acids.

Bioresour Technol 2021 Apr 15;333:125176. Epub 2021 Apr 15.

Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), 81531-970 Curitiba, PR, Brazil. Electronic address:

Omega-3 produced by marine thraustochytrids has appeared as an alternative to fish oil and an eco-friendly solution to overfishing. Herein, an integrative analysis of metagenetics and high-throughput screening was used for bioprospecting marine thraustochytrids from southern Brazil mangrove and coastal seawater. All sampled environments showed biodiversity and abundance of SAR clade. Read More

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Butyribacter intestini gen. nov., sp. nov., a butyric acid-producing bacterium of the family Lachnospiraceae isolated from human faeces, and reclassification of Acetivibrio ethanolgignens as Acetanaerobacter ethanolgignens gen. nov., comb. nov.

Syst Appl Microbiol 2021 Mar 30;44(3):126201. Epub 2021 Mar 30.

BGI-Shenzhen, Shenzhen 518083, China; Shenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome, BGI-Shenzhen, Shenzhen, China; Qingdao-Europe Advanced Institute for Life Sciences, BGI-Shenzhen, Qingdao 266555, China; BGI College & Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, China. Electronic address:

A novel, non-motile, Gram-stain-positive, non-spore-forming, obligate anaerobic bacterium, designated strain TF01-11, was isolated from human faeces. The isolate was characterized by phylogenetic and phenotypic properties, as well as by determination of its whole genome sequence. The growth temperature and pH ranges were 30-42 °C and 6. Read More

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Anaerotalea alkaliphila gen. nov., sp. nov., an alkaliphilic, anaerobic, fermentative bacterium isolated from a terrestrial mud volcano.

Extremophiles 2021 May 23;25(3):301-309. Epub 2021 Apr 23.

Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.

Diversity of extremophilic microorganisms in mud volcanoes is largely unexplored. Here, we report the isolation of a novel alkaliphilic, mesophilic, fermentative bacterium (strain F-3ap) from a terrestrial mud volcano located at the Taman peninsula, Russia. Cells of strain F-3ap are Gram-stain-positive non-motile rods. Read More

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Isolation of fast-growing thraustochytrids and seasonal variation on the fatty acid composition of thraustochytrids from mangrove regions of Navi Mumbai, India.

J Environ Manage 2021 Apr 17;290:112597. Epub 2021 Apr 17.

Department of Food Engineering and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai, 400019, India. Electronic address:

This study was aimed to isolate fast-growing thraustochytrids and the influence of seasonal variation in fatty acid composition from the mangrove habitat. The thraustochytrids were isolated from fallen yellowish or green mangrove leaves, in four seasons, including winter, summer, rainy, and post rainy season in one year. The thraustochytrids were analyzed for biomass production, total lipid content, and fatty acid profile. Read More

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Pikeienuella piscinae gen. nov., sp. nov., a novel genus in the family Rhodobacteraceae.

J Microbiol 2021 Apr 20. Epub 2021 Apr 20.

Department of Microbiology, Pukyong National University, Busan, 48513, Republic of Korea.

A novel bacterium, designated strain RR4-56, was isolated from a biofilter of a seawater recirculating aquaculture system. The 16S rRNA gene sequence analysis showed that the isolate was closely related to Halovulum dunhuangense YYQ-30 (92.6%), Albimonas donghaensis DS2 (91. Read More

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Allelopathic effects of Ulva linza on marine phytoplankton and identification of the allelochemicals.

Environ Sci Pollut Res Int 2021 Apr 20. Epub 2021 Apr 20.

Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong, People's Republic of China, 266003.

Green tides have been increasing in frequency and severity in coastal areas in recent years, and thus, the adverse effects of green tides on the environment have attracted much research attention. Allelopathy is one of the most significant effects of green tide algae on the surrounding organisms. In this study, a series of experiments were developed to fully investigate the allelopathic effects of the green tide alga Ulva linza on two common coastal phytoplankton species and to isolate and identify the chemical compositions of the allelochemicals. Read More

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Evolution of physicochemical and antioxidant properties of whey protein isolate during fibrillization process.

Food Chem 2021 Apr 8;357:129751. Epub 2021 Apr 8.

Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Whey protein isolate (WPI) fibrils have great potential for applications in future food manufacture due to their improved properties. However, the evolution of their properties during fibrillization is still not fully understood. Here, we investigate variational characteristics of WPI fibrils during formation process. Read More

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Vibrio nitrifigilis sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll.

Antonie Van Leeuwenhoek 2021 Apr 17. Epub 2021 Apr 17.

Institute of Oceanography, National Taiwan University, P.O. Box 23-13, Taipei, 10617, Taiwan.

A nitrogen-fixing isolate of facultatively anaerobic, marine bacterium, designated strain NFV-1, was recovered from the lagoon sediment of Dongsha Island, Taiwan. It was a Gram-negative rod which exhibited motility with monotrichous flagellation in broth cultures. The strain required NaCl for growth and grew optimally at about 25-35 °C, 3% NaCl and pH 7-8. Read More

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Massilia aromaticivorans sp. nov., a  BTEX Degrading Bacterium Isolated from Arctic Soil.

Curr Microbiol 2021 May 17;78(5):2143-2150. Epub 2021 Apr 17.

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.

A novel BTEX degrading bacterial strain, designated ML15P13, was isolated from Arctic soil at the Svalbard Islands, Norway, using an enrichment culture technique. This isolate is Gram-negative, aerobic, motile with multiple flagella at one polar end, and rod-shaped. Growth was observed at 4-35 °C, pH 6. Read More

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Silylium Ions: From Elusive Reactive Intermediates to Potent Catalysts.

Chem Rev 2021 Apr 16. Epub 2021 Apr 16.

Institut für Chemie, Technische Universität Berlin, Strasse des 17 Juni 115, 10623 Berlin, Germany.

The history of silyl cations has all the makings of a drama but with a happy ending. Being considered reactive intermediates impossible to isolate in the condensed phase for decades, their actual characterization in solution and later in solid state did only fuel the discussion about their existence and initially created a lot of controversy. This perception has completely changed today, and silyl cations and their donor-stabilized congeners are now widely accepted compounds with promising use in synthetic chemistry. Read More

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Isolation of Extracellular Vesicles Derived from Mesenchymal Stromal Cells by Ultracentrifugation.

Bio Protoc 2020 Dec 20;10(24):e3860. Epub 2020 Dec 20.

Laboratori Experimental de Nefrologia i Trasplantament (LENIT), Institut d'Investigacions Biomèdiques August Pi iSunyer (IDIBAPS), Barcelona, Spain.

Extracellular vesicles (EVs) are a heterogeneous group of membranous vesicles that differ on their biogenesis and release pathways, such as exosomes, microvesicles and apoptotic bodies. They are involved in cell-to-cell communication delivering signal molecules (proteins, nucleic acids, lipids, ) that can regulate different physiological processes, as well as the development and progression of several diseases. There are different methods and commercial kits to isolate EVs and depending on the methodology one could obtain EVs with different degrees of efficiency, purity and it can be more or less time-consuming. Read More

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December 2020