87,615 results match your criteria acids identified

The potential of immature poken () extract in the weight management, lipid and glucose metabolism.

J Complement Integr Med 2021 May 10. Epub 2021 May 10.

Research & Design Center, TCI CO., Ltd., Taipei, Taiwan.

Objectives: The prevalence of obesity was increasing globally, with nearly half a billion of the world's population now considered to be overweight or obese. The immature poken () was a good source of flavonoids and phenolic acids, which may exert an anti-obesity effect. However, the current efficacy in clinical trials was still unclear. Read More

View Article and Full-Text PDF

Perspective: Framework for Developing Recommended Intakes of Bioactive Dietary Substances.

Adv Nutr 2021 May 7. Epub 2021 May 7.

Weaver & Associates Consulting, LLC, Colorado Springs, CO, USA.

Dietary bioactives are food substances that promote health but are not essential to prevent typical deficiency conditions. Examples include lutein and zeaxanthin, omega-3 fatty acids, and flavonoids. When quality evidence is available, quantified intake recommendations linking dietary bioactives with specific health benefits will enable health professionals to provide evidence-based information to consumers. Read More

View Article and Full-Text PDF

Super-complex mixtures of aliphatic- and aromatic acids may be common degradation products after marine oil spills: A lab-study of microbial oil degradation in a warm, pre-exposed marine environment.

Environ Pollut 2021 Apr 28;285:117264. Epub 2021 Apr 28.

Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark. Electronic address:

When assessing oil spills in marine environments, focus has often been on describing degradation and removal of hydrocarbons. However, more and more attention is now given to the formation of mineral oil transformation products, and their potential toxicity and persistency in the environment. The aim of this study was to investigate the formation of dissolved acidic degradation products from crude oil in sea water from the Persian Gulf in a lab-experiment. Read More

View Article and Full-Text PDF

UVC-induced degradation of cilastatin in natural water and treated wastewater.

Chemosphere 2021 Apr 27;280:130668. Epub 2021 Apr 27.

Laboratory of Aquatic Chemistry, School of Environmental Engineering, Technical University of Crete, GR-73100, Chania, Crete, Greece. Electronic address:

This work reports for the first time the UVC photodegradation of cilastatin, a renal dehydropeptidase inhibitor co-adminstered with the imipenem antibiotic. Initially, solutions of cilastatin at varying concentrations were prepared in ultra-pure water and the direct photolysis of cilastatin was monitored under 254-nm irradiation. Degradation was slower at higher initial cilastatin concentrations, due to absorption saturation. Read More

View Article and Full-Text PDF

Myomixer is expressed during embryonic and post-larval hyperplasia, muscle regeneration and differentiation of myoblats in rainbow trout (Oncorhynchus mykiss).

Gene 2021 May 4:145688. Epub 2021 May 4.

INRAE, UR1037 Laboratory of Fish Physiology and Genomics, 35000 Rennes, France. Electronic address:

In contrast to mice or zebrafish, trout exhibits post-larval muscle growth through hypertrophy and formation of new myofibers (hyperplasia). The muscle fibers are formed by the fusion of mononucleated cells (myoblasts) regulated by several muscle-specific proteins such as Myomaker or Myomixer. In this work, we identified a unique gene encoding a Myomixer protein of 77 amino acids (aa) in the trout genome. Read More

View Article and Full-Text PDF

Targeting lipid metabolism is an emerging strategy to enhance the efficacy of anti-HER2 therapies in HER2-positive breast cancer.

Cancer Lett 2021 May 4. Epub 2021 May 4.

Medical Oncology Unit, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Venezian 1, 20133, Milan, Italy; IFOM, the FIRC Institute of Molecular Oncology, Via Adamello 16, Milan, Italy. Electronic address:

De novo or acquired resistance of cancer cells to currently available Human Epidermal Growth Factor Receptor 2 (HER2) inhibitors represents a clinical challenge. Several resistance mechanisms have been identified in recent years, with lipid metabolism reprogramming, a well-established hallmark of cancer, representing the last frontier of preclinical and clinical research in this field. Fatty Acid Synthase (FASN), the key enzyme required for fatty acids (FAs) biosynthesis, is frequently overexpressed/activated in HER2-positive (HER2+) breast cancer (BC), and it crucially sustains HER2+ BC cell growth, proliferation and survival. Read More

View Article and Full-Text PDF

SmKFB5 Protein Regulate Phenolic Acids Biosynthesis via Controlling the Degradation of Phenylalanine Ammonia-Lyase in Salvia miltiorrhiza.

J Exp Bot 2021 May 7. Epub 2021 May 7.

Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

Phenolic acids are the major secondary metabolites and significant bioactive constituents of medicinal plant Salvia miltiorrhiza. Many enzyme-encoding genes and transcription factors involved in phenolic acids biosynthesis have been identified, but the underlying posttranslational regulatory mechanisms are poorly understood. Here, we demonstrated that the S. Read More

View Article and Full-Text PDF

Effects of the Maillard reaction on the epitopes and immunoreactivity of tropomyosin, a major allergen in .

Food Funct 2021 May 7. Epub 2021 May 7.

College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, Fujian 361021, China.

Scallop (Chlamys nobilis) causes an IgE-mediated food allergy; however, studies of the allergens in its musculus are not sufficiently comprehensive. In this context, the target protein was purified from scallops and confirmed to be the major allergen tropomyosin (TM) using proteomic technology and serological testing. Subsequently, seven potential IgE epitopes of TM were obtained using phage display technology with IgE enrichment from the serum of scallop-sensitized patients and identified via inhibition enzyme-linked immunosorbent assays. Read More

View Article and Full-Text PDF

Evolutionary and functional analyses demonstrate conserved ferroptosis protection by Arabidopsis GPXs in mammalian cells.

FASEB J 2021 Jun;35(6):e21550

Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, China.

Species have evolved unique mechanisms to combat the effects of oxidative stress inside cells. A particularly devastating consequence of an unhindered oxidation of membrane lipids in the presence of iron results in cell death, known as ferroptosis. Hallmarks of ferroptosis, including peroxidation of polyunsaturated fatty acids, are conserved among animals and plants, however, early divergence of an ancestral mammalian GPX4 (mGPX4) has complicated our understanding of mechanistic similarities between species. Read More

View Article and Full-Text PDF

Multifaceted Roles of Adipose Tissue-Derived Exosomes in Physiological and Pathological Conditions.

Front Physiol 2021 20;12:669429. Epub 2021 Apr 20.

Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

Adipose tissue functions importantly in the bodily homeostasis and systemic metabolism, while obesity links to multiple disorders. Beyond the canonical hormones, growth factors and cytokines, exosomes have been identified to play important roles in transmission of information from adipose tissue to other organs. Exosomes are nanoscale membrane vesicles secreted by donor cells, and transfer the genetic information to the recipient cells where the encapsulated nucleic acids and proteins are released. Read More

View Article and Full-Text PDF

Metabolomic and phenotypic implications of the application of fertilization products containing microcontaminants in lettuce (Lactuca sativa).

Sci Rep 2021 May 6;11(1):9701. Epub 2021 May 6.

Department of Environmental Chemistry, Institute of Environmental, Assessment and Water Research (IDAEA), Spanish National Research Council (CSIC), c/Jordi Girona, 18-26, 08034, Barcelona, Spain.

Cultivation practice using organic amendments is plausible to ensure global food security. However, plant abiotic stress due to the presence of metals and organic microcontaminants (OMCs) in fertilization products cannot be overlooked. In this study, we monitored lettuce metabolism and phenotypic response following the application of either sewage sludge (SS), the organic fraction of municipal solid waste, swine manure (SM), chemical fertilizers (CF), or no amendment (C) in a greenhouse facility. Read More

View Article and Full-Text PDF

Plasma amino acids and oxylipins as potential multi-biomarkers for predicting diabetic macular edema.

Sci Rep 2021 May 6;11(1):9727. Epub 2021 May 6.

Department of Systems Biotechnology, Konkuk University, Seoul, Republic of Korea.

To investigate the pathophysiologic characteristics of diabetic complications, we identified differences in plasma metabolites in subjects with type 2 diabetes (T2DM) with or without diabetic macular edema (DME) and a disease duration > 15 years. An cohort of older T2DM patients with prolonged disease duration was established, and clinical information and biospecimens were collected following the guidelines of the National Biobank of Korea. DME phenotypes were identified by ophthalmologic specialists. Read More

View Article and Full-Text PDF

Impurity profiling of Compound Amino Acid Injection (6AA) using ion-pair high performance liquid chromatography coupled with corona-charged aerosol detection and high resolution mass spectrometry.

J Pharm Biomed Anal 2021 Apr 24;201:114099. Epub 2021 Apr 24.

Institute of Medicinal Biotechnology, Peking Union Medical College & Chinese Academy of Medical Sciences, No. 1, Tian Tan Xi Li, 100050, Beijing, PR China. Electronic address:

The complex industrial production process of amino acids (AAs) leads to the existence of a certain amount of impurities in Compound Amino Acid Injection (6AA). It is difficult to obtain its comprehensive and systematic impurity profile using conventional ultraviolet (UV) detectors due to lack of a suitable chromophore in the structures of AAs and their impurities. In our study, a universal ion-pair high performance liquid chromatography (HPLC) method combined with high resolution mass spectrometer (HRMS) and charged aerosol detection (CAD) was developed to identify and determine the content of impurities in Compound Amino Acid Injection (6AA), respectively. Read More

View Article and Full-Text PDF

A fibrinogen-related protein mediates the recognition of various bacteria and haemocyte phagocytosis in oyster Crassostrea gigas.

Fish Shellfish Immunol 2021 May 3. Epub 2021 May 3.

Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Southern Laboratory of Ocean Science and Engineering (Guangdong, Zhuhai), Zhuhai, 519000, China; Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.

The family of fibrinogen-related proteins (FREPs) is a group of proteins with fibrinogen-like (FBG) domains, which play important roles as pattern recognition receptors (PRRs) in the innate immune responses. In the present study, a fibrinogen-like protein was identified from the oyster Crassostrea gigas (defined as CgFREP1). The open reading frame of CgFREP1 was of 966 bp that encoded a predicted polypeptide of 321 amino acids comprising a signal peptide and a fibrinogen-like domain. Read More

View Article and Full-Text PDF

Heterocyclic amines formation and mitigation in processed meat and meat products: a review.

J Food Prot 2021 May 6. Epub 2021 May 6.

3Institute of Research and Development, Duy Tan University, Da Nang, 550000, Viet Nam 4Faculty of Environmental and Chemical Engineering, Duy Tan University, Da Nang, 550000, Viet Nam.

This review assesses heterocyclic amines (HCAs) formation and mitigation in processed meat and meat products. HCAs are formed when amino acids react with creatine during thermal processing of meat and meat products. The formation of HCAs depends on various factors, including the temperature and cooking time, fat contents, and the presence of HCA precursors, such as water, and lipids, meat marinating. Read More

View Article and Full-Text PDF

Integrating transcriptome and metabolome analyses of the response to cold stress in pumpkin (Cucurbita maxima).

PLoS One 2021 6;16(5):e0249108. Epub 2021 May 6.

Qingdao Institute of Agricultural Science Research, Qingdao, Shandong, China.

Cucurbita maxima belong to the genus Cucurbita and are of nutritional and economic importance. Physiological activity, transcriptome, and metabolome analyses of leaf samples from the C. maxima inbreding line IL7 treated at 5 °C and 25 °C were performed. Read More

View Article and Full-Text PDF

Guttation droplets of sugarcane red rot pathogen : Formation, toxigenic properties, and composition.

Mycologia 2021 May 6:1-11. Epub 2021 May 6.

Department of Soils and Environment, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625104, Tamil Nadu, India.

Production of guttation droplets in the cultures of was often noticed. Hence, formation of guttation droplets, exudation of toxin in guttation, and guttation droplet chemical composition were assessed to find the ecological importance of guttation. Among the tested culture media, more guttation was noticed in oat meal agar medium and host extracts broth medium. Read More

View Article and Full-Text PDF

Metabolomics in Glaucoma: A Systematic Review.

Invest Ophthalmol Vis Sci 2021 May;62(6)

School of Public Health, Medical College of Soochow University, Suzhou, China.

Purpose: Glaucoma remains a poorly understood disease, and identifying biomarkers for early diagnosis is critical to reducing the risk of glaucoma-related visual impairment and blindness. The aim of this review is to provide current metabolic profiles for glaucoma through a summary and analysis of reported metabolites associated with glaucoma.

Methods: We searched PubMed and Web of Science for metabolomics studies of humans on glaucoma published before November 11, 2020. Read More

View Article and Full-Text PDF

Molecular basis of various forms of maple syrup urine disease in Chilean patients.

Mol Genet Genomic Med 2021 May 6:e1616. Epub 2021 May 6.

Pediatrics Department, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.

Background: Maple syrup urine disease (MSUD) is an autosomal recessive inherited metabolic disorder caused by the deficient activity of the branched-chain α-keto acid dehydrogenase (BCKD) enzymatic complex. BCKD is a mitochondrial complex encoded by four genes: BCKDHA, BCKDHB, DBT, and DLD. MSUD is predominantly caused by mutations in the BCKDHA, BCKDHB, and DBT genes which encode the E1α, E1β, and E2 subunits of the BCKD complex, respectively. Read More

View Article and Full-Text PDF

Plasma lipid metabolites associate with diabetic polyneuropathy in a cohort with type 2 diabetes.

Ann Clin Transl Neurol 2021 May 6. Epub 2021 May 6.

Department of Neurology, University of Michigan, Ann Arbor, Michigan.

Objective: The global rise in type 2 diabetes is associated with a concomitant increase in diabetic complications. Diabetic polyneuropathy is the most frequent type 2 diabetes complication and is associated with poor outcomes. The metabolic syndrome has emerged as a major risk factor for diabetic polyneuropathy; however, the metabolites associated with the metabolic syndrome that correlate with diabetic polyneuropathy are unknown. Read More

View Article and Full-Text PDF

A natural knockout of the MYO7A gene leads to pre-weaning mortality in pigs.

Anim Genet 2021 May 6. Epub 2021 May 6.

Topigs Norsvin Research Center, Beuningen, The Netherlands.

The pig breeding system provides a unique framework to study recessive defects and the consequence on the phenotype. We examined a commercial synthetic Duroc population for recessive defects and identified a haplotype on chromosome 9 significantly affecting pre-weaning mortality. To identify the causal variant underlying the mortality, we examined sequence data of four carrier animals and 21 non-carrier animals from the same population. Read More

View Article and Full-Text PDF

Distinct identities of leaf phloem cells revealed by single cell transcriptomics.

Plant Cell 2021 May;33(3):511-530

Institute for Molecular Physiology and Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University Düsseldorf, Düsseldorf 40225, Germany.

The leaf vasculature plays a key role in solute translocation. Veins consist of at least seven distinct cell types, with specific roles in transport, metabolism, and signaling. Little is known about leaf vascular cells, in particular the phloem parenchyma (PP). Read More

View Article and Full-Text PDF

Analysis of Omega-3 Fatty Acid Content in Fish Oil Products.

J Pharm Pract 2021 May 6:8830738211015051. Epub 2021 May 6.

219251Presbyterian College School of Pharmacy, Clinton, SC, USA.

Background: Omega-3 fatty acids eicosapentaenoic acid (EPA) and docosehexaenoic acid (DHA), often found in fish oil supplements, have been linked to cardiovascular benefits in proper doses.

Objectives: Quantify serving sizes and EPA and DHA content of fish oil products and determine which products contain appropriate amounts of EPA and DHA per serving to lower cholesterol.

Methods: Products were identified through the National Institutes of Health's Dietary Supplement Label Database using the search term "fish oil. Read More

View Article and Full-Text PDF

Short-chain fatty acids-producing probiotics: A novel source of psychobiotics.

Crit Rev Food Sci Nutr 2021 May 6:1-31. Epub 2021 May 6.

Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

Psychobiotics-live microorganisms with potential mental health benefits, which can modulate the microbiota-gut-brain-axis via immune, humoral, neural, and metabolic pathways-are emerging as novel therapeutic options for the effective treatment of psychiatric disorders Recently, microbiome studies have identified numerous putative psychobiotic strains, of which short-chain fatty acids (SCFAs) producing bacteria have attracted special attention from neurobiologists. Recent studies have highlighted that SCFAs-producing bacteria such as , and have a very specific function in various psychiatric disorders, suggesting that these bacteria can be potential novel psychobiotics. SCFAs, potential mediators of microbiota-gut-brain axis, might modulate function of neurological processes. Read More

View Article and Full-Text PDF

Compound heterozygous p. Arg949Trp and p. Gly970Ala mutations deteriorated the function of PEX1p: A study on PEX1 in a patient with Zellweger syndrome.

J Cell Biochem 2021 May 6. Epub 2021 May 6.

Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Postal Code 81746-73441, Iran.

The peroxisome is responsible for a variety of vital pathways in primary metabolism, including the very long-chain fatty-acid oxidation and plasmalogen lipid biosynthesis. Autosomal recessive disorder of the Zellweger spectrum (ZSD) is a major subset of peroxisome biogenesis disorders (PBDs) that can be caused by mutations in any of the 14 PEX genes. Zellweger syndrome (ZS) is the foremost common and severe phenotype within the heterogeneous ZSD. Read More

View Article and Full-Text PDF

One-Pot Extractive Transesterification of Fatty Acids Followed by DMOX Derivatization for Location of Double Bonds Using GC-EI-MS.

Methods Mol Biol 2021 ;2306:105-121

Research School of Biology, Australian National University, Canberra, ACT, Australia.

Fatty acids are an essential structural and energy storage component of cells and hence there is much interest in their metabolism, requiring identification and quantification with readily available instrumentation, such as GC-MS. Fatty acid methyl esters (FAMEs) can be generated and extracted directly from biological tissue, in a one-pot process, and following high resolution GC, their respective chain length, degrees of unsaturation, and other functionalities can be readily identified using EI-MS. Defining the positions of the double bonds in the alkyl chain requires conversion of the FAMEs into their respective dimethyloxazoline (DMOX) derivatives. Read More

View Article and Full-Text PDF
January 2021

Impact of dietary mercury intake during pregnancy on the health of neonates and children: a systematic review.

Nutr Rev 2021 May 5. Epub 2021 May 5.

S. Saavedra and J.D. Gonzalez-Sanz are with the Department of Nursing, University of Huelva, Huelva, Spain. Á. Fernández-Recamales, A. Sayago, and R. González-Domínguez are with the AgriFood Laboratory, Faculty of Experimental Sciences, University of Huelva, Huelva, Spain; and the Agrifood Campus of International Excellence (ceiA3), University of Huelva, Huelva, Spain. A. Cervera-Barajas is with the Department of Nursing, University of Seville, Seville, Spain.

Context: Growing evidence suggests that prenatal exposure to methyl mercury through the maternal diet could have great influence on the neurological and physical development of neonates and young children.

Objective: The aim of this review was to evaluate the clinical repercussions of maternal exposure to methyl mercury during pregnancy on the health of fetuses, neonates, and children up to 8 years of age.

Data Sources: The Web of Science, PubMed, CINAHL, Scopus, and Dialnet Plus databases were searched for articles published in English or Spanish from 1990 to 2020. Read More

View Article and Full-Text PDF

Mutational Analysis of a Familial Adenomatous Polyposis Pedigree with Bile Duct Polyp Phenotype.

Can J Gastroenterol Hepatol 2021 12;2021:6610434. Epub 2021 Apr 12.

Shengli Clinical Medical College of Fujian Medical University, Fuzhou 350001, China.

A large number of colorectal cancers have a genetic background in China. However, due to insufficient awareness, the diagnostic rate remains low and merely 5-6% of colorectal cancer patients are diagnosed with hereditary colorectal cancer. Familial adenomatous polyposis (FAP) is an autosomal dominant genetic disease caused by mutations in the adenomatous polyposis coli (APC) gene. Read More

View Article and Full-Text PDF

Noninvasive monitoring of evolving urinary metabolic patterns in neonatal encephalopathy.

Pediatr Res 2021 May 5. Epub 2021 May 5.

Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.

Background: Infants with moderate and severe neonatal encephalopathy (NE) frequently suffer from long-term adverse outcomes. We hypothesize that the urinary metabolome of newborns with NE reflects the evolution of injury patterns observed with magnetic resonance imaging (MRI).

Methods: Eligible patients were newborn infants with perinatal asphyxia evolving to NE and qualifying for therapeutic hypothermia (TH) included in the HYPOTOP trial. Read More

View Article and Full-Text PDF

[Pharmacological Interaction between Diets and Commensal Bacteria for the Creation of Lipid Environment in the Control of Health and Diseases].

Yakugaku Zasshi 2021 ;141(5):681-688

Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research, and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition.

The intestine is exposed to a variety of exogenous materials that are harmful, harmless, or useful, such as pathogenic viruses and bacteria, intestinal bacteria, or food components. As such, the intestinal immune system is important for the regulation of immunological homeostasis and biological defense. Accumulating evidence indicates that gut environmental factors, such as dietary components and intestinal bacteria are critical for controlling intestinal immunity, and thereby, health and disease. Read More

View Article and Full-Text PDF
January 2021