104,043 results match your criteria acidic conditions


Rapid analysis of phenyl isothiocyanate derivatives of amino acids present in Czech meads.

J Chromatogr A 2021 Apr 3;1644:462134. Epub 2021 Apr 3.

University of Pardubice, Faculty of Chemical Technology, Department of Analytical Chemistry, Studentská 573, CZ-53210 Pardubice, Czech Republic. Electronic address:

Amino acids (AAs) are minor compounds occurring in meads contributing to their final organoleptic properties. Determination of AAs profile and content can help to assess the mead authenticity, adulteration and thus its quality. This work deals with the optimization of rapid analysis of 21 AAs present in mead using reversed-phase high performance liquid chromatography with spectrophotometric detection after simple derivatization procedure with phenyl isothiocyanate agent without any sample pre-treatment. Read More

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Triacylglycerol lipidome from human meibomian gland epithelial cells: Description, response to culture conditions, and perspective on function.

Exp Eye Res 2021 Apr 10:108573. Epub 2021 Apr 10.

University of Alabama at Birmingham, School of Optometry, Department of Optometry and Vision Science, Birmingham, AL, USA.

Preliminary work has shown that select triacylglycerols (TAGs) are upregulated in a preclinical model of MGD, suggesting that TAGs may be an important outcome variable in research involving human meibomian gland epithelial cells (HMGECs). The purpose of this study was to explore the HMGEC TAG lipidome in culture conditions known to influence differentiation. HMGECs were differentiated in DMEM/F12 with 10 ng/ml EGF, FBS (2% or 10%), and rosiglitazone (0, 20, or 50 μM) for two or five days. Read More

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Soils from abandoned shooting range facilities as contamination source of potentially toxic elements: distribution among soil geochemical fractions.

Environ Geochem Health 2021 Apr 13. Epub 2021 Apr 13.

Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208, Matosinhos, Portugal.

Civilian and military shooting range facilities cause environmental issues in several countries due to the accumulation of Potentially Toxic Elements; as a result of weathering of ammunitions accumulated into the soils. The contents and distribution of Cu, Ni, Pb and Zn were analyzed in 12 soils in an abandoned clay target shooting range at two different depths (0-15 and 15-30 cm). Single extractions (CaCl and DTPA) and Tessier sequential extraction were conducted to assess the PTE mobility and the PTE distribution in the different soil geochemical fractions at both depths. Read More

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Synthesis of β-Hydroxy-α,α-difluorosulfonamides from Carbanions of Difluoromethanesulfonamides.

J Org Chem 2021 Apr 13. Epub 2021 Apr 13.

Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.

The synthesis of β-hydroxy-α,α-difluorosulfonamides was achieved by reacting difluoromethanesulfonamides with KHMDS in the presence of an aldehyde or ketone. The reaction exhibited a dramatic counterion effect with KHMDS or NaHMDS usually giving excellent yields in minutes, while lithium bases gave little or no product. Excellent yields and high diastereomeric ratios were achieved with -benzyl--phenylfluorenyl (PhF)-protected chiral amino aldehydes derived from amino acids. Read More

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Intestinal microbiota drives cholestasis-induced specific hepatic gene expression patterns.

Gut Microbes 2021 Jan-Dec;13(1):1-20

Translational Research Laboratory, Gastroenterology and Hepatology, Ente Ospedaliero Cantonale, Università Della Svizzera Italiana, Lugano, Switzerland.

Intestinal microbiota regulates multiple host metabolic and immunological processes. Consequently, any difference in its qualitative and quantitative composition is susceptible to exert significant effects, in particular along the gut-liver axis. Indeed, recent findings suggest that such changes modulate the severity and the evolution of a wide spectrum of hepatobiliary disorders. Read More

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Characterization of an L-α,β-diaminopropionic acid polymer with comb-like structure isolated from a poly(ε-L-lysine)-producing Streptomyces sp.

Appl Microbiol Biotechnol 2021 Apr 12. Epub 2021 Apr 12.

Department of Life Science and Biotechnology, Graduate School of Science and Engineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka, 564-8680, Japan.

Polymers of basic amino acids function as polycationic compounds under physiological conditions and exhibit intriguing biological properties, such as antimicrobial and antiviral activities, immunopotentiating ability, and DNA-binding activity. Poly(ε-L-lysine) (ε-PL) produced by some strains of Streptomyces spp. is a cationic homopolymer of L-lysine linking between ε-amino and α-carboxylic acid functional groups and has been used as a food preservative based on its biocompatibility and biodegradability. Read More

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Triple SILAC identified progestin-independent and dependent PRA and PRB interacting partners in breast cancer.

Sci Data 2021 Apr 12;8(1):100. Epub 2021 Apr 12.

Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.

Progesterone receptor (PR) isoforms, PRA and PRB, act in a progesterone-independent and dependent manner to differentially modulate the biology of breast cancer cells. Here we show that the differences in PRA and PRB structure facilitate the binding of common and distinct protein interacting partners affecting the downstream signaling events of each PR-isoform. Tet-inducible HA-tagged PRA or HA-tagged PRB constructs were expressed in T47DC42 (PR/ER negative) breast cancer cells. Read More

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Intrahepatic Cholestasis of Pregnancy Is Associated with Reduced Nitric Oxide Synthase (iNOS) in Plasma and Placentas: A Pilot Study.

Med Sci Monit 2021 Apr 13;27:e930176. Epub 2021 Apr 13.

Department of Obstetrics and Gynecology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, China (mainland).

BACKGROUND Intrahepatic cholestasis of pregnancy (ICP) is a condition specific to pregnancy, leading to increased fetal morbidity and mortality. Nitric oxide synthase (iNOS) may be a factor regulating the vasodilation of blood vessels, which are relevant to ischemic-hypoxic conditions. We aimed to explore the potential relationship between iNOS and ICP. Read More

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Halotolerant bacteria mitigate the effects of salinity stress on soybean growth by regulating secondary metabolites and molecular responses.

BMC Plant Biol 2021 Apr 12;21(1):176. Epub 2021 Apr 12.

School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.

Background: Salinity is a major threat to the agriculture industry due to the negative impact of salinity stress on crop productivity. In the present study, we isolated rhizobacteria and evaluated their capacities to promote crop growth under salt stress conditions.

Results: We isolated rhizospheric bacteria from sand dune flora of Pohang beach, Korea, and screened them for plant growth-promoting (PGP) traits. Read More

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Astrogliosis and episodic memory in late life: higher GFAP is related to worse memory and white matter microstructure in healthy aging and Alzheimer's disease.

Neurobiol Aging 2021 Feb 26;103:68-77. Epub 2021 Feb 26.

Department of Neurology, University of Colorado Alzheimer's & Cognition Center, Linda Crnic Institute for Down Syndrome, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.

Astrocytes play a formative role in memory consolidation during physiological conditions; when dysregulated, astrocytes release glial fibrillary acidic protein (GFAP), which has been linked with negative memory outcomes in animal studies. We examined the association between blood GFAP, memory, and white matter (WM) integrity, accounting for blood markers of AD pathology (i.e. Read More

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February 2021

Impact of moderate exercise on fatty acid oxidation in pancreatic β-cells and skeletal muscle.

J Endocrinol Invest 2021 Apr 12. Epub 2021 Apr 12.

Centre Européen D'étude du Diabète, Unité Mixte de Recherche de L'Université de Strasbourg « Diabète et Thérapeutique », Strasbourg, France.

Fatty acids (FA) play a crucial role in glycaemia regulation in healthy and metabolic disorders conditions through various mechanisms. FA oxidation is one of the processes involved in lipid metabolism and can be modulated by exercise. Nowadays, physical activity is known to be an effective strategy for the prevention and treatment of Type 2 Diabetes. Read More

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Physiological effects of nutrients on insulin release by pancreatic beta cells.

Mol Cell Biochem 2021 Apr 12. Epub 2021 Apr 12.

Grupo de investigación en Biología celular y funcional e ingeniería de biomoléculas, Universidad Antonio Nariño-Sede Circunvalar. Cra, 3 Este # 47A - 15, Bl 5, Bogotá, Colombia.

Obesity and type 2 diabetes (T2D) are growing health problems associated with a loss of insulin sensitivity. Both conditions arise from a long-term energy imbalance, and frequently, lifestyle measures can be useful in its prevention, including physical activity and a healthy diet. Pancreatic β-cells are determinant nutrient sensors that participate in energetic homeostasis needs. Read More

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Effect of pH on the performance of an acidic biotrickling filter for simultaneous removal of HS and siloxane from biogas.

Water Sci Technol 2021 Apr;83(7):1511-1521

Ebara Jitsugyo Co., Ltd., Ginza, Chuo-ku, Tokyo, Japan.

Acidic biotrickling filters (BTF) can be used for simultaneous removal of hydrogen sulfide (HS) and siloxane from biogas. In this study, the performance of a BTF under different acidic pH conditions was investigated. The removal profile of HS showed that 90% of HS removal was achieved during the first 0. Read More

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Call for More Effective Regulation of Clinical Trials with Advanced Therapy Medicinal Products Consisting of or Containing Genetically Modified Organisms in the European Union.

Hum Gene Ther 2021 Apr 11. Epub 2021 Apr 11.

Biogen Idec Inc, 2191, Regulatory Chemistry, Manufacturing and Controls, Maidenhead, Berkshire, United Kingdom of Great Britain and Northern Ireland;

Advanced Therapy Medicinal Products (ATMPs), such as gene therapies that consist of or contain Genetically Modified Organisms (GMOs) need to comply with the European Union (EU) GMO legislation, as implemented in each EU Member State, before a clinical trial can commence. Complying with GMO requirements is complex, varies significantly across EU Member States and is leading to delays to clinical trials with ATMPs (see Supplemental materials). Such delays and varying implementation of the GMO legislation makes the EU less attractive as a region to conduct clinical trials with investigational gene therapies. Read More

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Cometabolic Vinyl Chloride Degradation at Acidic pH Catalyzed by Acidophilic Methanotrophs Isolated from Alpine Peat Bogs.

Environ Sci Technol 2021 Apr 12. Epub 2021 Apr 12.

Department of Civil and Environmental Engineering, KAIST, Daejeon 34141, South Korea.

Remediation of toxic chlorinated ethenes via microbial reductive dechlorination can lead to ethene formation; however, the process stalls in acidic groundwater, leading to the accumulation of carcinogenic vinyl chloride (VC). This study explored the feasibility of cometabolic VC degradation by moderately acidophilic methanotrophs. Two novel isolates, strain JS1 and strain MJC1, were obtained from distinct alpine peat bogs located in South Korea. Read More

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GPR120/FFAR4 Pharmacology: Focus on Agonists in Type 2 Diabetes Mellitus Drug Discovery.

J Med Chem 2021 Apr 10. Epub 2021 Apr 10.

Department of Biotechnology, Chemistry, and Pharmacy, DoE 2018-2022, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.

The G-protein coupled receptors (GPCRs) activated by free fatty acids (FFAs) have emerged as new and exciting drug targets, due to their plausible translation from pharmacology to medicines. This perspective aims to report recent research about GPR120/FFAR4 and its involvement in several diseases, including cancer, inflammatory conditions, and central nervous system disorders. The focus is to highlight the importance of GPR120 in Type 2 diabetes mellitus (T2DM). Read More

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Iodinated BSA Nanoparticles for Macrophage-Mediated CT Imaging and Repair of Gastritis.

Anal Chem 2021 Apr 12. Epub 2021 Apr 12.

State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China.

The development of a specific and noninvasive technology for understanding gastritic response together with efficient therapy is an urgent clinical issue. Herein, we fabricated a novel iodinated bovine serum albumin (BSA) nanoparticle based on gastritic microenvironment for computed tomography (CT) imaging and repair of acute gastritis. Derived from the characteristic mucosa defect and inflammatory cell (e. Read More

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Chitin Deacetylation Using Deep Eutectic Solvents: -Supported Process Optimization.

ACS Sustain Chem Eng 2021 Mar 5;9(10):3874-3886. Epub 2021 Mar 5.

Department of Catalysis and Chemical Reaction Engineering, National Institute of Chemistry, Hajdrihova ulica 19, 1000 Ljubljana, Slovenia.

Chitin is the most abundant marine biopolymer, being recovered during the shell biorefining of crustacean shell waste. In its native form, chitin displays a poor reactivity and solubility in most solvents due to its extensive hydrogen bonding. This can be overcome by deacetylation. Read More

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Effects of water deficit on leaves and fruit quality during the development period in tomato plant.

Food Sci Nutr 2021 Apr 16;9(4):1949-1960. Epub 2021 Feb 16.

INRA-Centre d'Avignon UR1115 Plantes et Systèmes de Culture Horticoles Avignon France.

In nature, plants are often exposed to a multitude of environmental constraints that severely limit crop productivity. Water deficit is one of the factors that most affects agricultural production. The aim of this work is to evaluate the effect of water deficit on morphology, development, nutritional behavior, as well as chlorophyll fluorescence and certain important metabolic parameters (soluble sugars, organic acids, starch, carotenoid, and vitamin C) of the cultivated tomato ( cv Plovdiv). Read More

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Laser-induced graphene for bioelectronics and soft actuators.

Nano Res 2021 Apr 7:1-18. Epub 2021 Apr 7.

Department of Biomedical, Biological & Chemical Engineering, University of Missouri, Columbia, Missouri 65211 USA.

Laser-assisted process can enable facile, mask-free, large-area, inexpensive, customizable, and miniaturized patterning of laser-induced porous graphene (LIG) on versatile carbonaceous substrates (e.g., polymers, wood, food, textiles) in a programmed manner at ambient conditions. Read More

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