569 results match your criteria acid-treated mice

Octanoic acid a major component of widely consumed medium-chain triglyceride ketogenic diet is detrimental to bone.

Sci Rep 2021 Mar 26;11(1):7003. Epub 2021 Mar 26.

Department of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, 110062, India.

Octanoic acid is a medium-chained saturated fatty acid found abundantly in the ketogenic dietary supplements containing medium chained triglycerides (MCT) along with decanoic acid. The MCT ketogenic diet is commonly consumed for weight loss but has also showcased neuroprotective potential against neurodegenerative disorders. However, recent clinical findings have reported a critical disadvantage with the long-term consumption of ketogenic diet i. Read More

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Bacteroides ovatus Promotes IL-22 Production and Reduces Trinitrobenzene Sulfonic Acid-Driven Colonic Inflammation.

Am J Pathol 2021 04 28;191(4):704-719. Epub 2021 Jan 28.

Department of Pediatrics, Baylor College of Medicine, Houston, Texas; Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas; Department of Pathology, Texas Children's Hospital, Houston, Texas.

The intestinal microbiota influences the development and function of the mucosal immune system. However, the exact mechanisms by which commensal microbes modulate immunity is not clear. We previously demonstrated that commensal Bacteroides ovatus ATCC 8384 reduces mucosal inflammation. Read More

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Allodynia by Splenocytes From Mice With Acid-Induced Fibromyalgia-Like Generalized Pain and Its Sexual Dimorphic Regulation by Brain Microglia.

Front Neurosci 2020 23;14:600166. Epub 2020 Dec 23.

Department of Pharmacology and Therapeutic Innovation, Nagasaki University Institute of Biomedical Sciences, Nagasaki, Japan.

Fibromyalgia (FM), a disease of unknown etiology characterized by chronic generalized pain, is partly recapitulated in an animal model induced by repeated acid saline injections into the gastrocnemius muscle. Here, we attempted to investigate the sex difference in pain hypersensitivity (mechanical allodynia and hypersensitivity to electrical stimulation) in the repeated acid saline-induced FM-like generalized pain (AcGP) model. The first unilateral acid injection into gastrocnemius muscle at day 0/D0 and second injection at D5 (post day 0, P0) induced transient and long-lasting mechanical allodynia, respectively, on both sides of male and female mice. Read More

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December 2020

Tranexamic Acid Improves Memory and Learning Abilities in Aging Mice.

J Exp Pharmacol 2020 18;12:653-663. Epub 2020 Dec 18.

R&D Department, Daiichi Sankyo Healthcare Co., LTD, Shinagawa-ku, Tokyo 103-8234, Japan.

Purpose: Although the onset mechanism of Alzheimer's disease, which co-occurs with aging, has been extensively studied, no effective methods that improve the decline in memory and learning abilities following aging have been developed. Tranexamic acid provided promising results for ameliorating photo-aging and extending the natural lifespan. However, it is unknown whether it affects the decline in memory and learning abilities due to aging. Read More

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December 2020

Chemical pancreatectomy treats chronic pancreatitis while preserving endocrine function in preclinical models.

J Clin Invest 2021 Feb;131(3)

Division of Pediatric Surgery.

Chronic pancreatitis affects over 250,000 people in the US and millions worldwide. It is associated with chronic debilitating pain, pancreatic exocrine failure, and high risk of pancreatic cancer and usually progresses to diabetes. Treatment options are limited and ineffective. Read More

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February 2021

Unripe Miquel Extract Containing Ellagic Acid Promotes Lipolysis and Thermogenesis In Vitro and In Vivo.

Molecules 2020 Dec 16;25(24). Epub 2020 Dec 16.

Central R&D Center, Bioresources and Technology (B&Tech) Co., Ltd., Gwangju 61239, Korea.

Previously, we demonstrated that a 5% ethanol extract of unripe (5-RCK) and ellagic acid has hypocholesterolemic and antiobesity activity, at least partially mediated by the downregulation of adipogenic and lipogenic gene expression in high-fat diet (HFD)-fed animals. The present study investigated the thermogenic and lipolytic antiobesity effects of 5-RCK and ellagic acid in HFD-induced obese C57BL/6 mice and explored its mechanism of action. Mice fed an HFD received 5-RCK or ellagic acid as a post-treatment or pretreatment. Read More

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December 2020

Activation of the AMPK-SIRT1 pathway contributes to protective effects of Salvianolic acid A against lipotoxicity in hepatocytes and NAFLD in mice.

Front Pharmacol 2020 30;11:560905. Epub 2020 Nov 30.

Molecular Medicine Institute, Zhejiang Chinese Medical University, Hangzhou, China.

Salvianolic acid A (Sal A), a natural polyphenol compound extracted from (known as Danshen in China), possesses a variety of potential pharmacological activities. The aim of this study is to determine mechanisms of hepatoprotective effects of Sal A against lipotoxicity both in cultured hepatocytes and in a mouse model of fatty liver disease. High-fat and high-carbohydrate diet (HFCD)-fed C57BL/6J mice were employed to establish hepatic lipotoxicity in a mouse model. Read More

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November 2020

Histidine-rich Glycoprotein alleviate Ischemia/Reperfusion Liver Injury in Mice with Nonalcoholic Steatohepatitis.

Liver Transpl 2020 Dec 1. Epub 2020 Dec 1.

Center for Advanced Medical Innovation, Kyushu University, Fukuoka, Japan.

Background And Aims: Hepatic ischemia/reperfusion (I/R) injury is a major complication of liver surgery and transplantation, especially in non-alcoholic steatohepatitis (NASH). The mechanism of NASH susceptibility to I/R injury has not been fully clarified. We investigated the role of liver-produced histidine-rich glycoprotein (HRG) in NASH I/R injury. Read More

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December 2020

A comparative study of the phenotype with kainic acid-induced seizure in DBA/2 mice from three different sources.

Lab Anim Res 2020 28;36:39. Epub 2020 Oct 28.

Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Republic of Korea.

The kainic acid-induced seizure mouse model is widely used in epilepsy research. In this study, we applied kainic acid to the subcutaneous injections of three different sources of DBA/2 mice to compare and evaluate the seizure response. The three mouse sources consisted of DBA/2Kor1 (Korea FDA source), DBA/2A (USA source), and DBA/2 (Japan source), and were purchased from different vendors. Read More

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October 2020

Cinnamtannin D1 Protects Pancreatic β-Cells from Glucolipotoxicity-Induced Apoptosis by Enhancement of Autophagy In Vitro and In Vivo.

J Agric Food Chem 2020 Nov 30;68(45):12617-12630. Epub 2020 Oct 30.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

In our previous study, cinnamtannin D1 (CD-1), one of the A-type procyanidin oligomers isolated from , was reported to have the activity of antiapoptosis in palmitic acid-treated pancreatic β cells via alleviating oxidative stress in vitro. In this study, the aim was to further disclose its protective effect and underlying mechanisms against glucolipotoxicity-induced β-cells apoptosis in vitro and in vivo. We found that CD-1 was able to dose-dependently and time-dependently activate autophagy in INS-1 pancreatic βcells. Read More

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November 2020

S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.

Cell Mol Gastroenterol Hepatol 2021 17;11(3):697-724. Epub 2020 Oct 17.

Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming, Yunnan, China; Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China; Department of Hepatobiliary Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China. Electronic address:

Background & Aims: Nonalcoholic fatty liver disease (NAFLD) is becoming a severe liver disorder worldwide. Autophagy plays a critical role in liver steatosis. However, the role of autophagy in NAFLD remains exclusive and under debate. Read More

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October 2020

Atraric Acid Exhibits Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW264.7 Cells and Mouse Models.

Int J Mol Sci 2020 Sep 25;21(19). Epub 2020 Sep 25.

Department of Pharmacy, Sunchon National University, 255 Jungang-Ro, Suncheon 549-742, Korea.

Lichens, composite organisms resulting from the symbiotic association between the fungi and algae, produce a variety of secondary metabolites that exhibit pharmacological activities. This study aimed to investigate the anti-inflammatory activities of the secondary metabolite atraric acid produced by . The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264. Read More

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September 2020

Punicalagin improves hepatic lipid metabolism modulation of oxidative stress and mitochondrial biogenesis in hyperlipidemic mice.

Food Funct 2020 Nov;11(11):9624-9633

Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, Shaanxi, China.

Hyperlipidemia is closely associated with various liver diseases, and effective intervention for prevention and treatment is in great need. Here, we aim to explore the protective effects of punicalagin (PU), a major ellagitannin in pomegranate, on acute hyperlipidemia-induced hepatic lipid metabolic disorders. Male C57bl/6J mice were pretreated with 50 or 200 mg kg-1 day-1 PU for 9 days before the injection of poloxamer 407 to induce acute hyperlipidemia. Read More

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November 2020

Diet containing stearic acid increases food reward-related behaviors in mice compared with oleic acid.

Brain Res Bull 2020 11 18;164:45-54. Epub 2020 Aug 18.

Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangzhou, Guangdong 510642, China; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China. Electronic address:

Obesity is currently a worldwide phenomenon. The consumption of calorie-rich foods is responsible for most obesity cases, but not all humans exposed to high-calorie diets develop obesity. According to recent studies, exposure to fat-rich diets may be the actual cause of obesity. Read More

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November 2020

A heritable profile of six miRNAs in autistic patients and mouse models.

Sci Rep 2020 06 9;10(1):9011. Epub 2020 Jun 9.

Erciyes University, Betul-Ziya Eren Genome and Stem Cell Center, Kayseri, Turkey.

Autism spectrum disorder (ASD) is a group of developmental pathologies that impair social communication and cause repetitive behaviors. The suggested roles of noncoding RNAs in pathology led us to perform a comparative analysis of the microRNAs expressed in the serum of human ASD patients. The analysis of a cohort of 45 children with ASD revealed that six microRNAs (miR-19a-3p, miR-361-5p, miR-3613-3p, miR-150-5p, miR-126-3p, and miR-499a-5p) were expressed at low to very low levels compared to those in healthy controls. Read More

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Allogeneic Bone Marrow Mesenchymal Stem Cell Transplantation in Tooth Extractions Sites Ameliorates the Incidence of Osteonecrotic Jaw-Like Lesions in Zoledronic Acid-Treated Rats.

J Clin Med 2020 May 31;9(6). Epub 2020 May 31.

Research Group Cellular Therapy and Hematopoietic Transplant, Biomedical Research Institute, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, Avenida Buenavista s/n, 30120 Murcia, Spain.

Medication-related osteonecrosis of the jaw (MRONJ) is defined as the exposed necrotic bone involving the maxillofacial structures in bisphosphonate treated patients, and the pathophysiology of this disease remains unclear. The aim of this study was to assess the effects of the allogeneic transplantation of bone marrow-derived mesenchymal stem cells (BM-MSCs) in a model of Wistar mice with induced MRONJ disease. BM-MSCs from five male Wistar rats were characterized and cultured on β-tricalcium phosphate (β-TCP) granules. Read More

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Effect of Glycyrrhizic Acid on Scopolamine-Induced Cognitive Impairment in Mice.

Int Neurourol J 2020 May 31;24(Suppl 1):S48-55. Epub 2020 May 31.

Department of Biomedical Laboratory Science, Catholic Kwandong University, Gangneung, Korea.

Purpose: Cognitive impairment is one of the main symptoms of Alzheimer disease and other dementias. Glycyrrhiza uralensis is a natural product that has a protective effect against cognitive impairment. In this study, we investigated whether glycyrrhizic acid, among the main bioactive components of Glycyrrhiza uralensis, has a neuroprotective effect on scopolamine-induced cognitive impairment. Read More

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Effect of Deglycosylated Rutin by Acid Hydrolysis on Obesity and Hyperlipidemia in High-Fat Diet-Induced Obese Mice.

Nutrients 2020 May 25;12(5). Epub 2020 May 25.

School of Food Biotechnology and Nutrition, Kyungsung University, Busan 48434, Korea.

The present study evaluated the effects of acid-treated rutin on hyperlipidemia and obesity in high-fat diet (HFD)-induced obese mice. The mice consumed a HFD with or without acid-treated rutin for 7 weeks. Body weight gain considerably decreased, by approximately 33%, in the acid-treated rutin (AR) and quercetin (Q) groups compared to that in the HFD group. Read More

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Bile acids, lipid and purine metabolism involved in hepatotoxicity of first-line anti-tuberculosis drugs.

Expert Opin Drug Metab Toxicol 2020 Jun 21;16(6):527-537. Epub 2020 May 21.

Department of Clinical Pharmacology, The First Affiliated Hospital of Soochow University , Suzhou, China.

Objectives: Rifampin (RIF), isoniazid (INH) and pyrazinamide (PZA) are essential components of the short-term first-line anti-tuberculosis (anti-TB) chemotherapy regimen and can cause hepatotoxicity. However, the mechanism of anti-TB drug-induced hepatotoxicity (ATDH) is currently unclear. We investigate the relevant contributions to liver injury and the pathway of the above-mentioned drugs administered alone or in combination. Read More

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Inhibition of Sphingosine-1-Phosphate-Induced Th17 Cells Ameliorates Alcohol-Associated Steatohepatitis in Mice.

Hepatology 2021 Mar;73(3):952-967

Department of Medicine, University of Louisville, Louisville, KY.

Background And Aims: Chronic alcohol consumption is accompanied by intestinal inflammation. However, little is known about how alterations to the intestinal immune system and sphingolipids contribute to the pathogenesis of alcohol-associated liver disease (ALD).

Approach And Results: We used wild-type mice, retinoid-related orphan receptor gamma t (RORγt)-deficient mice, sphingosine kinase-deficient mice, and local gut anti-inflammatory, 5-aminosalicyclic acid-treated mice in a chronic-binge ethanol feeding model. Read More

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Cyanidin-3-O-glucoside improves non-alcoholic fatty liver disease by promoting PINK1-mediated mitophagy in mice.

Br J Pharmacol 2020 08 14;177(15):3591-3607. Epub 2020 Jun 14.

Key Laboratory of Zoonoses Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Background And Purpose: Identifying safe and effective compounds that target to mitophagy to eliminate impaired mitochondria may be an attractive therapeutic strategy for non-alcoholic fatty liver disease. Here, we investigated the effects of cyanidin-3-O-glucoside (C3G) on non-alcoholic fatty liver disease (NAFLD) and the underlying mechanism.

Experimental Approach: Non-alcoholic fatty liver disease was induced by a high-fat diet for 16 weeks. Read More

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Mst1 knockdown alleviates cardiac lipotoxicity and inhibits the development of diabetic cardiomyopathy in db/db mice.

Biochim Biophys Acta Mol Basis Dis 2020 08 19;1866(8):165806. Epub 2020 Apr 19.

Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. Electronic address:

Diabetic cardiomyopathy (DCM) accounts for increasing deaths of diabetic patients, and effective therapeutic targets are urgently needed. Myocardial lipotoxicity, which is caused by cardiac non-oxidative metabolic fatty acids and cardiotoxic fatty acid metabolites accumulation, has gained more attention to explain the increasing prevalence of DCM. However, whether mammalian Ste20-like kinase 1 (Mst1) plays a role in lipotoxicity in type 2 diabetes-induced cardiomyopathy has not yet been illustrated. Read More

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miRNA-mRNA profile and regulatory network in stearic acid-treated β-cell dysfunction.

J Endocrinol 2020 07;246(1):13-27

Department of Nutrition and Food Hygiene (National Key Discipline), Public Health College, Harbin Medical University, Harbin, China.

Chronic exposure of pancreatic β-cells to saturated fatty acid (palmitic or stearic acid) is a leading cause of impaired insulin secretion. However, the molecular mechanisms underlying stearic-acid-induced β-cell dysfunction remain poorly understood. Emerging evidence indicates that miRNAs are involved in various biological functions. Read More

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Asiatic Acid Inhibits OVX-Induced Osteoporosis and Osteoclastogenesis Regulating RANKL-Mediated NF-κb and Nfatc1 Signaling Pathways.

Front Pharmacol 2020 27;11:331. Epub 2020 Mar 27.

School of Biomedical Sciences, the University of Western Australia, Perth, WA, Australia.

is a triterpenoid compound extracted from a medicinal plant . It has been used as a highly efficient compound for the treatment of cancer and hyperlipidemia, as well as possessing potential antiinflammatory properties. However, its effects on bone metabolism and osteoporosis haven't been reported. Read More

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miR-21 promotes osseointegration and mineralization through enhancing both osteogenic and osteoclastic expression.

Mater Sci Eng C Mater Biol Appl 2020 Jun 27;111:110785. Epub 2020 Feb 27.

Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China.

The demand for orthopedic implants continues to increase with the aging population. As the most widely used orthopedic materials, titanium and its alloys have achieved high success rates. However, the lack of bone tissue integration remains a barrier to successful operations. Read More

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Long noncoding RNA lncARSR promotes nonalcoholic fatty liver disease and hepatocellular carcinoma by promoting YAP1 and activating the IRS2/AKT pathway.

J Transl Med 2020 03 13;18(1):126. Epub 2020 Mar 13.

Department of Radiology, Shengjing Hospital of China Medical University, No. 36, Sanhao Street, Heping District, Shenyang, 110004, Liaoning, People's Republic of China.

Background: Nonalcoholic fatty liver disease (NAFLD) is the main cause for hepatocellular carcinoma (HCC). This study was intended to identify the function of long non-coding RNA (lncRNA) lncARSR in NAFLD and its role in human HCC cells (HepG2) proliferation and invasion.

Methods: LncARSR expression was detected both in high fatty acid-treated HepG2 cells and NAFLD mouse model. Read More

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Lymphatic endothelial cells promote T lymphocyte migration into lymph nodes under hyperlipidemic conditions.

Biochem Biophys Res Commun 2020 05 5;525(3):786-792. Epub 2020 Mar 5.

Department of Microbiology, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea. Electronic address:

Lymphatic vessels serve as conduits through which immune cells traffic. Because lymphatic vessels are also involved in lipid transport, their function is vulnerable to abnormal metabolic conditions such as obesity and hyperlipidemia. Exactly how these conditions impact immune cell trafficking, however, is not well understood. Read More

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The role of cellular prion protein in lipid metabolism in the liver.

Prion 2020 12;14(1):95-108

Department of Neurology, Clinical Dementia Center, University Medical Center Göttingen and German Center for Neurodegenerative Diseases (DZNE), Göttingen, Germany.

Cellular prion protein (PrPC) is a plasma membrane glycophosphatidylinositol-anchored protein and it is involved in multiple functions, including neuroprotection and oxidative stress. So far, most of the PrPC functional research is done in neuronal tissue or cell lines; the role of PrPC in non-neuronal tissues such as liver is only poorly understood. To characterize the role of PrPC in the liver, a proteomics approach was applied in the liver tissue of PrPC knockout mice. Read More

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December 2020

Indigenous Microbiota Protects against Inflammation-Induced Osteonecrosis.

J Dent Res 2020 06 28;99(6):676-684. Epub 2020 Feb 28.

The Shapiro Family Laboratory of Viral Oncology and Aging Research, UCLA School of Dentistry, Los Angeles, CA, USA.

Medication-related osteonecrosis of the jaw (MRONJ) is a rare intraoral lesion that occurs in patients undergoing long-term and/or high-dose therapy with nitrogen-containing bisphosphonates, a RANKL inhibitor, antiangiogenic agents, or mTOR inhibitors. The presence of pathogenic bacteria is highly associated with advanced stages of MRONJ lesions; however, the exact role of indigenous microbes in MRONJ development is unknown. Here, we report that the normal oral flora in mice protects against inflammation-induced osteonecrosis. Read More

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Improved in-solution trypsin digestion method for methanol-chloroform precipitated cellular proteomics sample.

J Sep Sci 2020 Jun 9;43(11):2125-2132. Epub 2020 Mar 9.

Department of Chemistry and Biochemistry, University of Texas, Arlington, Texas, USA.

Methanol-chloroform based protein precipitation is an essential step in many liquid chromatography-tandem mass spectrometry-based cellular proteomics applications. However, re-solubilization of the total protein precipitate is difficult using regular in-solution digestion protocol. Sodium deoxycholate is reported as an efficient surfactant for re-solubilization of membrane fractions. Read More

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