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Pharmaceutics 2021 Feb 17;13(2). Epub 2021 Feb 17.

Department of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea.

Polydimethylsiloxane (PDMS) is a biocompatible polymer that has been applied in many fields. However, the surface hydrophobicity of PDMS can limit successful implementation, and this must be reduced by surface modification to improve biocompatibility. In this study, we modified the PDMS surface with a hydrogel and investigated the effect of this on hydrophilicity, bacterial adhesion, cell viability, immune response, and biocompatibility of PDMS. Read More

Theranostics 2021 6;11(8):3948-3960. Epub 2021 Feb 6.

Toronto General Hospital Research Institute, Division of Cardiovascular Surgery, University Health Network, Toronto, Ontario, Canada.

Pacemaker implantation is currently used in patients with symptomatic bradycardia. Since a pacemaker is a lifetime therapeutic device, its energy consumption contributes to battery exhaustion, along with its voltage stimulation resulting in local fibrosis and greater resistance, which are all detrimental to patients. The possible resolution for those clinical issues is an injection of a conductive hydrogel, poly-3-amino-4-methoxybenzoic acid-gelatin (PAMB-G), to reduce the myocardial threshold voltage for pacemaker stimulation. Read More

Food Funct 2021 Feb;12(3):1087-1096

Flinders Institute for Nanoscale Science and Technology, College of Science and Engineering, Flinders University, Bedford Park, South Australia, 5042, Australia.

We have developed a simple process for the entrapment of nutrients in shear stress induced non-covalent physically entangled tannic acid-gelatin gel in a thin film vortex fluidic device (VFD) operating under continuous flow. This allows control of the porosity and surface area of the pores in order to improve the nutrient entrapment capacity. The VFD microfluidic platform simplifies the processing procedure of physically entangled biopolymers, as a time and cost saving one-step process devoid of any organic solvents, in contrast to the conventional homogenization process, which is also inherently complex, involving multiple-step processing. Read More

Mater Sci Eng C Mater Biol Appl 2021 Feb 17;119:111639. Epub 2020 Oct 17.

Research Unit of Advanced, Composite, Nanomaterials and Nanotechnology, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str., Zografou Campus, 15780 Athens, Greece.

Additive manufacturing holds promise for the fabrication of three-dimensional scaffolds with precise geometry, to serve as substrates for the guided regeneration of natural tissue. In this work, a bioinspired approach is adopted for the synthesis of hybrid hydroxyapatite hydrogels, which were subsequently printed to form 3D scaffolds for bone tissue engineering applications. These hydrogels consist of hydroxyapatite nanocrystals, biomimetically synthesized in the presence of both chitosan and l-arginine. Read More

Int J Biol Macromol 2021 Jan 18;167:1598-1612. Epub 2020 Nov 18.

School of Pharmaceutical Science, South-Central University for Nationalities, Wuhan 430074, China; National Demonstration Center for Experimental Ethnopharmacology Education, South-Central University for Nationalities, Wuhan 430074, China. Electronic address:

A major drawback of oral treatment of inflammatory bowel disease (IBD) is the non-specific distribution of drugs during long-term treatment. Despite its effectiveness as an anti-inflammatory drug, curcumin (CUR) is limited by its low bioavailability in IBD treatment. Herein, a pH-sensitive composite hyaluronic acid/gelatin (HA/GE) hydrogel drug delivery system containing carboxymethyl chitosan (CC) microspheres loaded with CUR was fabricated for IBD treatment. Read More

Acta Biomater 2020 12 25;118:83-99. Epub 2020 Aug 25.

Department of Prosthodontics, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. Electronic address:

The guided bone regeneration (GBR) concept has been extensively utilized to treat maxillofacial bone defects in clinical practice. However, the repair efficacy of currently available GBR membranes is often compromised by their limited bone regeneration potential and deficient antibacterial activity. In this study, inspired by the bi-layered structure design of the commonly used Bio-Gidemembrane, we designed and fabricated a new kind of multifunctional bi-layered "GBR scaffold" combining solution electrospinning writing (SEW) and solution electrospinning (SES) techniques using a single SEW printer. Read More

Biomater Sci 2020 Aug 24;8(15):4225-4238. Epub 2020 Jun 24.

Department of Endocrinology and Metabolism, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.

Diabetic wounds remain a serious clinical challenge whereas current therapies have limited effects on reducing the high disability and morbidity. Impaired vascularization is closely associated with delayed healing of diabetic wounds and liraglutide (Lira), a GLP-1R receptor agonist, has been reported to promote the angiogenic ability of endothelial cells. However, its application is hindered owing to the unsustainable drug concentration. Read More

J Funct Biomater 2020 Jun 3;11(2). Epub 2020 Jun 3.

Institute of Cell Biophysics, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", 142290 Pushchino, Moscow Region, Russia.

The extracellular cell surface-associated and soluble heat shock protein 90 (Hsp90) is known to participate in the migration and invasion of tumor cells. Earlier, we demonstrated that plasma membrane-associated heparan sulfate proteoglycans (HSPGs) bind the extracellular Hsp90 and thereby promote the Hsp90-mediated motility of tumor cells. Here, we showed that a conjugate of 2,5-dihydroxybenzoic acid with gelatin (2,5-DHBA-gelatin), a synthetic polymer with heparin-like properties, suppressed the basal (unstimulated) migration and invasion of human glioblastoma A-172 and fibrosarcoma HT1080 cells, which was accompanied by the detachment of a fraction of Hsp90 from cell surface HSPGs. Read More

Curr Mol Pharmacol 2020 May 4. Epub 2020 May 4.

Department of Pharmaceutics, ISF College of Pharmacy, Moga-144002 (Punjab). India.

Purpose: Formulation, optimization and anticancer activity of spray-dried Doxorubicin loaded folic acid conjugated Gelatin nanoparticles (DOX-FA-GN).

Method: Doxorubicin loaded gelatin nanoparticles (DOX-GN) were prepared by the Coacervation phase separation method, optimized using DoE and then conjugated with folic acid by covalent coupling to formulate Doxorubicin loaded folic acid conjugated nanoparticles (DOX-FA-GN). The formulated nanoparticles were characterized to evaluate its physicochemical properties. Read More

Bioelectrochemistry 2020 Aug 18;134:107536. Epub 2020 Apr 18.

Centre for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, Valencia, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valencia, Spain.

Electrical stimulation (ES) has provided enhanced chondrogenesis of mesenchymal stem cells (MSCs) cultured in micro-mass without the addition of exogenous growth factors. In this study, we demonstrate for the first time that ES of MSCs encapsulated in an injectable hyaluronic acid (HA) - gelatin (GEL) mixture enhances the chondrogenic potential of the hydrogel. Samples were stimulated for 21 days with 10 mV/cm at 60 kHz, applied for 30 min every 6 h a day. Read More

Fluids Barriers CNS 2020 Feb 11;17(1):16. Epub 2020 Feb 11.

Fischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, 3110 A. James Clark Hall, College Park, MD, 20742, USA.

Background: The endothelial cell-cell junctions of the blood-brain barrier (BBB) play a pivotal role in the barrier's function. Altered cell-cell junctions can lead to barrier dysfunction and have been implicated in several diseases. Despite this, the driving forces regulating junctional protein presentation remain relatively understudied, largely due to the lack of efficient techniques to quantify their presentation at sites of cell-cell adhesion. Read More

Int J Biol Macromol 2020 Apr 22;148:777-784. Epub 2020 Jan 22.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China; College of Tea combined with Food Science and Technology, Anhui Agricultural University, Hefei 230036, China; International Joint Laboratory on Tea Chemistry and Health Effects of Ministry of Education, Hefei 230036, China. Electronic address:

The wounds of diabetic patients are difficult to heal, which could lead to a limb amputation or even death. The experiment aims to develop a new type of nanoparticles that could accelerate wound healing. Epigallocatechin gallate, ascorbic acid, gelatin and chitosan nanoparticles (EV NPS) were prepared by ion cross-linking method, and their properties were studied. Read More

Food Chem 2020 May 24;313:126078. Epub 2019 Dec 24.

College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Ningbo Research Institute, Zhejiang University, Hangzhou 315100, China. Electronic address:

Effects of Chlorogenic acid-Gelatin (CGA-Gel) combined with partial freezing on quality change of sword prawn (Parapenaeopsis hardwickii) stored at -5 °C were evaluated for 23 days. Changes in sensory score, total viable counts (TVC), and physiochemical indexes including pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid reactive substances (TBARS) and Ca-ATPase were examined. All shrimp treated with CGA and CGA-Gel had lower total viable counts compared to control (P < 0. Read More

FASEB J 2020 01 5;34(1):1710-1727. Epub 2019 Dec 5.

Brain Tumour Research Centre, Institute of Biological and Biomolecular Sciences (IBBS), School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK.

Despite the importance of the tumor microenvironment in regulating tumor progression, few in vitro models have been developed to understand the effects of non-neoplastic cells and extracellular matrix (ECM) on drug resistance in glioblastoma (GBM) cells. Using CellTrace-labeled human GBM and microglial (MG) cells, we established a 2D co-culture including various ratios of the two cell types. Viability, proliferation, migration, and drug response assays were carried out to assess the role of MG. Read More

Int J Mol Sci 2019 Nov 29;20(23). Epub 2019 Nov 29.

Brain Tumour Research Centre, Institute of Biological and Biomedical Sciences (IBBS), School of Pharmacy and Biomedical Sciences, University of Portsmouth, White Swan Road, Portsmouth PO1 2DT, UK.

The role of astrocytes in the glioblastoma (GBM) microenvironment is poorly understood; particularly with regard to cell invasion and drug resistance. To assess this role of astrocytes in GBMs we established an all human 2D co-culture model and a 3D hyaluronic acid-gelatin based hydrogel model (HyStem™-HP) with different ratios of GBM cells to astrocytes. A contact co-culture of fluorescently labelled GBM cells and astrocytes showed that the latter promotes tumour growth and migration of GBM cells. Read More

J Cell Biochem 2020 03 23;121(3):2077-2088. Epub 2019 Oct 23.

Department of Periodontology and Implantology, Faculty of Dental Sciences and Centre for Regenerative Medicine and Stem Cell Research, Sri Ramachandra Medical College and Research Institute, Chennai, India.

Spinal cord injury induces scar formation causes axonal damage that leads to the degeneration of axonal function. Still, there is no robust conceptual design to regenerate the damaged axon after spinal injury. Therefore, the present study demonstrates that human gingival derived neuronal stem cells (GNSCs) transplants in the injectable caffeic acid bioconjugated hydrogel (CBGH) helps to bridge the cavity and promote the engraftment and repopulation of transplants in the injured spinal tissue. Read More

ACS Omega 2019 Jun 28;4(6):11293-11300. Epub 2019 Jun 28.

Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt.

The cytotoxic potential of allicin was evaluated on different cancer cell lines, particularly, hepatic (HepG-2), breast (MCF-7), lung (A-549), and prostatic (PC-3), where allicin scored an IC score of 19.26 μM on HepG-2. In order to increase the cell uptake, optimized allicin-loaded gelatin nanoparticles (GNPs) were prepared where the optimum formulation was surface-conjugated to glycyrrhetinic acid. Read More

Int J Biol Macromol 2019 Nov 23;140:959-964. Epub 2019 Aug 23.

Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address:

Flaxseed oil, due to high amount of unsaturation, is susceptible to oxidation which could reduce oil nutritional value and cause off-flavor. In this study, flaxseed oil was encapsulated in new wall materials of tertiary conjugate of gelatin-flaxseed mucilage (FM)-oxidized tannic acid (OTA) which are available and food grade with nutritional value, controlled-release ability. The effect of different percentages of oil (15, 30 and 50% w/w concentration of total dry matter) on encapsulation efficiency (EE) and loading (EL) was investigated. Read More

Biomolecules 2019 08 5;9(8). Epub 2019 Aug 5.

Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.

Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields. In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering. In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels. Read More

Carbohydr Polym 2019 Jul 14;215:189-197. Epub 2019 Mar 14.

Marmara University, Department of Chemical Engineering, 34722, Istanbul, Turkey. Electronic address:

Herein, electroconductive polymeric films consisting of hyaluronic acid (HyA), gelatin (Gel), poly(ethylene oxide) (PEO) reinforced by reduced graphene oxide (RGO) were used in drug release studies to investigate usability of the films as drug carrier in the future. Irbesartan (IRB) used for the treatment of cardiovascular diseases was loaded to the polymeric films and its release kinetic was investigated. Afterwards, the obtained controlled drug release data were simulated using different dynamic differential mathematical models such as 1, 2, 3 degree and Higuchi model. Read More

Mater Sci Eng C Mater Biol Appl 2019 May 9;98:628-634. Epub 2019 Jan 9.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.. Electronic address:

Tissue sealants are used for hemorrhage control which is imperative in many surgical procedures. It is a highly challenging task to obtain the ideal tissue sealant. Only a few commercially tissue sealants are available to be used for internal tissue or organ hemorrhage control. Read More

J Cell Physiol 2019 03 24;234(3):1967-1977. Epub 2018 Aug 24.

Department of Periodontology and Implantology, Faculty of Dental Sciences, Centre for Regenerative Medicine and Stem Cell Research, Sri Ramachandra Medical College and Research Institute, Porur, India.

Present-day scaffolds are useful in cell therapy to a reasonable extent, but in pursuit of improvising the scaffold to improve the outcome, we tested a new injectable caffeic acid-bioconjugated gelatin hydrogel scaffold (CBGH; with tunable stiffness -10%). Two-dimensional (2D) form of human umbilical cord tissue-derived mesenchymal stem cells (HUCMSCs) culture performed based on our previously reported methods and characterized by using multipotent and pluripotent analysis. In addition, neurogenesis was induced in the presence of retinoic acid or neural growth factor or epidermal growth factor categorized by neuronal markers. Read More

J Cancer Res Ther 2018 Jan-Mar;14(2):287-291

Department of Physics, Medical Gel Dosimetry Laboratory, School of Advanced Sciences, VIT University, Vellore, Tamil Nadu, India.

Aims: The aim of this study is to evaluate the dose rate and energy dependency of methacrylic acid gelatin tetrakis (hydroxymethyl) phosphonium chloride (MAGAT) gel in unflattened photon beam using X-ray computed tomography (CT) and ultraviolet (UV)-visible spectroscopy.

Materials And Methods: MAGAT gel was prepared and it was exposed to 6 MV flattened and unflattened beams. The dose selected for irradiation was ranging from 3 to 15 Gy with an increment of 3 Gy. Read More

Medicine (Baltimore) 2017 Dec;96(52):e9437

Department of Interventional Radiology Department of Radiology Department of Pain, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.

To evaluate the different arteriographic manifestations of acute arterial massive hemorrhage of the gastrointestinal (GI) tract and the efficacy of emergency transcatheter arterial embolization (ETAE).A total of 88 patients with acute massive GI bleeding who experienced failure of initial endoscopy and/or conservative treatment were referred to our interventional department for acute GI arteriography from January 2007 to June 2015. After locating the source of bleeding, appropriate embolic agents, such as spring coil, hydroxyl methyl acrylic acid gelatin microspheres, polyvinyl alcohol (PVA) particles, etc. Read More

Cell Prolif 2018 Apr 15;51(2):e12407. Epub 2017 Nov 15.

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237, China.

Objectives: Stem cell factor (SCF) is considered as a commonly indispensable cytokine for proliferation of haematopoietic stem cells (HSCs), which is used in large dosages during ex vivo culture. The work presented here aimed to reduce the consumption of SCF by sustained release but still support cells proliferation and maintain the multipotency of HSCs.

Materials And Methods: Stem cell factor was physically encapsulated within a hyaluronic acid/gelatin double network (HGDN) hydrogel to achieve a slow release rate. Read More

Biopolymers 2018 Feb 15;109(2). Epub 2017 Nov 15.

Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka, 560-8531, Japan.

Bioprinting has a great potential to fabricate three-dimensional (3D) functional tissues and organs. In particular, the technique enables fabrication of 3D constructs containing stem cells while maintaining cell proliferation and differentiation abilities, which is believed to be promising in the fields of tissue engineering and regenerative medicine. We aimed to demonstrate the utility of the bioprinting technique to create hydrogel constructs consisting of hyaluronic acid (HA) and gelatin derivatives through irradiation by visible light to fabricate 3D constructs containing human adipose stem cells (hADSCs). Read More

J Biomater Appl 2018 01 13;32(6):775-787. Epub 2017 Nov 13.

1 Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1 Ssangyoung-Dong, Cheonan, South Korea.

The objective of the present study was to develop a novel hybrid multichannel biphasic calcium phosphate granule (MCG)-based composite system for cartilage regeneration. First, hyaluronic acid-gelatin (HG) hydrogel was coated onto MCG matrix (MCG-HG). Poly(lactic-co-glycolic acid) (PLGA) microspheres was separately prepared and modified with polydopamine subsequent to BMP-7 loading (B). Read More

Biopolymers 2018 Jan 6;109(1). Epub 2017 Nov 6.

Department of Molecular Science and Technology, Ajou University, Suwon, Republic of Korea.

Development of bioadhesives with tunable mechanical strength, high adhesiveness, biocompatibility, and injectability is greatly desirable in all surgeries to replace or complement the sutures and staples. Herein, the dual catalytic activity of horseradish peroxidase is exploited to in situ form the hydroxyphenyl propionic acid-gelatin/thiolated gelatin (GH/GS) adhesive hydrogels including two alternative crosslinks (phenol-phenol and disulfide bonds) with fast gelation (few seconds - several minutes) and improved physicochemical properties. Their elastic moduli increase from 6. Read More

J Biomater Appl 2017 Oct 24;32(4):433-445. Epub 2017 Sep 24.

1 Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Chungnam, Cheonan City, Republic of Korea.

An ideal bone substitute should be made of biocompatible materials that mimic the structure, characteristics, and functions of natural bone. Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system. In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure. Read More

Materials (Basel) 2017 Jul 19;10(7). Epub 2017 Jul 19.

Department of Integrative Engineering, Chung-Ang University, Seoul 156756, Korea.

In this work, crosslinkers were prepared by conjugating high- and low-molecular-weight gelatin with different mole ratios of itaconic acid (IA) with double bonds. Then, the gelatin-itaconic acid (gelatin-IA) crosslinkers were compared with the gelatin-methacrylate (gelatin-MA) crosslinkers. The molecular weights and structures of gelatin-MA and gelatin-IA were confirmed using gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR). Read More