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Employing metabolomic approaches to determine the influence of age on experimental autoimmune encephalomyelitis (EAE).

Mol Immunol 2021 Apr 16;135:84-94. Epub 2021 Apr 16.

Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea; Division of Bio-Medical Science & Technology, KIST-School, Korea University of Science and Technology (UST), Seoul, 02792, Republic of Korea. Electronic address:

The immune system plays a critical role not only in homeostasis of the body but also in pathogenesis. Autoimmunity and dysregulation of the immune balance are closely related to age. To examine the influence of age on autoimmunity, the pathophysiological features of experimental autoimmune encephalomyelitis (EAE) induced at different ages were elucidated on the basis of plasma-level metabolic changes. Read More

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Comparative genomic analysis of the genus Novosphingobium and the description of two novel species Novosphingobium aerophilum sp. nov. and Novosphingobium jiangmenense sp. nov.

Syst Appl Microbiol 2021 Mar 30;44(3):126202. Epub 2021 Mar 30.

Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, P.R. China. Electronic address:

Members of the genus Novosphingobium are well known for their metabolically versatile and great application potential in pollution elimination. The three novel bacterial strains, designated 4Y4, 4Y9, and 1Y9A, were isolated from aquaculture water and characterized by using a polyphasic taxonomic approach. The 16S rRNA gene sequences phylogenetic analysis revealed that the three strains belonged to the genus Novosphingobium. Read More

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Antimicrobial susceptibility pattern of oral gram negative anaerobes from Indian subjects.

Anaerobe 2021 Apr 16:102367. Epub 2021 Apr 16.

Central Research Laboratory, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Bauxite Road, Belgaum, Karnataka, India.

Objectives: There is paucity of information on the antimicrobial susceptibility pattern of oral anaerobic bacteria. In this study, an attempt has been made to evaluate the antimicrobial susceptibility/resistance trend of oral Gram negative bacteria from Indian subjects.

Methods: Minimum inhibitory concentrations (MIC) of 304 isolates against twelve different antibiotics were determined using gradient diffusion MIC strips. Read More

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Streptomyces adelaidensis sp. nov., an actinobacterium isolated from the root of Callitris preissii with potential for plant growth-promoting properties.

Arch Microbiol 2021 Apr 19. Epub 2021 Apr 19.

Department of Medical Biotechnology, College of Medicine and Public Health, Flinders University, Adelaide, Australia.

An endophytic actinobacterium, strain CAP261 was isolated from the surface sterilized root of Callitris preissii (Australian native pine tree). As a result of a polyphasic taxonomy study, this strain was identified as a member of the genus Streptomyces. This strain was an aerobic actinobacterium with well-developed substrate mycelia with loop spore chains and the spore surfaces are verrucose. Read More

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DNA Methylation at Birth is Associated with Childhood Serum Immunoglobulin E Levels.

Epigenet Insights 2021 5;14:25168657211008108. Epub 2021 Apr 5.

Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Miaoli.

Immunoglobulin E (IgE) is known to play an important role in allergic diseases. Epigenetic traits acquired due to modification of deoxyribonucleic acid (DNA) methylation (DNAm) in early life may have phenotypic consequences through their role in transcriptional regulation with relevance to the developmental origins of diseases including allergy. However, epigenome-scale studies on the longitudinal association of cord blood DNAm with IgE over time are lacking. Read More

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Halobellus ruber sp. nov., a deep red-pigmented extremely halophilic archaeon isolated from a Korean solar saltern.

Antonie Van Leeuwenhoek 2021 Apr 17. Epub 2021 Apr 17.

Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon, 22012, Republic of Korea.

A novel halophilic archaeon, strain MBLA0160, was isolated from a solar saltern in Sorae, Republic of Korea. The cells are deep-red pigmented, Gram-negative, rod shaped, motile, and lysed in distilled water. The strain MBLA0160 grew at 25-45 °C (optimum 37 °C), in 15-30% (w/v) NaCl (optimum 20%) and 0. Read More

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Vibrio nitrifigilis sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll.

Antonie Van Leeuwenhoek 2021 Apr 17. Epub 2021 Apr 17.

Institute of Oceanography, National Taiwan University, P.O. Box 23-13, Taipei, 10617, Taiwan.

A nitrogen-fixing isolate of facultatively anaerobic, marine bacterium, designated strain NFV-1, was recovered from the lagoon sediment of Dongsha Island, Taiwan. It was a Gram-negative rod which exhibited motility with monotrichous flagellation in broth cultures. The strain required NaCl for growth and grew optimally at about 25-35 °C, 3% NaCl and pH 7-8. Read More

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Massilia aromaticivorans sp. nov., a  BTEX Degrading Bacterium Isolated from Arctic Soil.

Curr Microbiol 2021 Apr 17. Epub 2021 Apr 17.

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.

A novel BTEX degrading bacterial strain, designated ML15P13, was isolated from Arctic soil at the Svalbard Islands, Norway, using an enrichment culture technique. This isolate is Gram-negative, aerobic, motile with multiple flagella at one polar end, and rod-shaped. Growth was observed at 4-35 °C, pH 6. Read More

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Hypoglycemic attacks and growth failure are the most common manifestations of citrin deficiency after one year of age.

J Inherit Metab Dis 2021 Apr 16. Epub 2021 Apr 16.

Department of Pediatrics, Tohoku University School of Medicine, Sendai, Japan.

Citrin deficiency develops in different symptomatic periods from the neonatal period to adulthood. Some infantile patients are diagnosed by newborn mass screening or symptoms of neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD), some patients in childhood may develop hepatopathy or dyslipidemia as failure to thrive and dyslipidemia caused by citrin deficiency (FTTDCD), and some adults are diagnosed after developing adult-onset type 2 citrullinemia (CTLN2) with hyperammonemia or encephalopathy. A diagnosis is needed before the development of severe phenotypic CTLN2 but is often difficult to obtain because newborn mass screening cannot detect all patients with citrin deficiency, and undiagnosed patients often appear healthy in childhood. Read More

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Microbacterium helvum sp. nov., a novel actinobacterium isolated from cow dung.

Arch Microbiol 2021 Apr 16. Epub 2021 Apr 16.

Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.

A Gram-positive, aerobic, non-motile, non-spore-forming, short rod-shaped strain, NEAU-LLC, was isolated from cow dung in Shangzhi City, Heilongjiang Province, Northeast China and identified by a polyphasic taxonomic study. Colonies was light yellow, round, with entire margin. Strain NEAU-LLC was grown at 15-45 ℃ and pH 6. Read More

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Halomonas maris sp. nov., a moderately halophilic bacterium isolated from sediment in the southwest Indian Ocean.

Arch Microbiol 2021 Apr 15. Epub 2021 Apr 15.

Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China.

A halophilic, Gram-staining-negative, rod-shaped, flagellated and motile bacterium, strain QX-1, was isolated from deep-sea sediment at a depth of 3332 m in the southwestern Indian Ocean. Strain QX-1 growth was observed at 4-50 °C (optimum 37 °C), pH 5.0-11. Read More

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Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress.

NPJ Biofilms Microbiomes 2021 Apr 13;7(1):34. Epub 2021 Apr 13.

Genetics of Biofilms Laboratory, Institut Pasteur, UMR CNRS2001, Paris, France.

Communities of bacteria called biofilms are characterized by reduced diffusion, steep oxygen, and redox gradients and specific properties compared to individualized planktonic bacteria. In this study, we investigated whether signaling via nitrosylation of protein cysteine thiols (S-nitrosylation), regulating a wide range of functions in eukaryotes, could also specifically occur in biofilms and contribute to bacterial adaptation to this widespread lifestyle. We used a redox proteomic approach to compare cysteine S-nitrosylation in aerobic and anaerobic biofilm and planktonic Escherichia coli cultures and we identified proteins with biofilm-specific S-nitrosylation status. Read More

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mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2.

BMC Microbiol 2021 Apr 13;21(1):111. Epub 2021 Apr 13.

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.

Background: Streptococcus suis type 2 (SS2) is an important zoonotic pathogen. We have previously reported the structure of LuxS protein and found that the luxS gene is closely related to biofilm, virulence gene expression and drug resistance of SS2. However, the mechanism of luxS mediated SS2 stress response is unclear. Read More

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sp. nov., isolated from blood of an immunocompromised patient.

Int J Syst Evol Microbiol 2021 Apr;71(4)

Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Republic of Korea.

A novel Gram-stain-negative, facultative aerobic and rod-shaped bacterium, designated as MKL-01 and isolated from the blood of immunocompromised patient, was genotypically and phenotypically characterized. The colonies were found to be creamy yellow and convex. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that strain MKL-01 was most closely related to LMG 5886, present within a large cluster in the genus . Read More

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gen. nov., sp. nov., a novel marine bacterium isolated from a bacterial consortium that degrades polyethylene terephthalate.

Int J Syst Evol Microbiol 2021 Apr;71(4)

Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of China, State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.

A novel Gram-stain-negative, aerobic, gliding, rod-shaped and carotenoid-pigmented bacterium, designated A20-9, was isolated from a microbial consortium of polyethylene terephthalate enriched from a deep-sea sediment sample from the Western Pacific. Growth was observed at salinities of 1-8 %, at pH 6.5-8 and at temperatures of 10-40 °C. Read More

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A combined RAD-Seq and WGS approach reveals the genomic basis of yellow color variation in bumble bee Bombus terrestris.

Sci Rep 2021 Apr 12;11(1):7996. Epub 2021 Apr 12.

Department of Biology, The Pennsylvania State University, 208 Mueller Labs, University Park, PA, USA.

Bumble bees exhibit exceptional diversity in their segmental body coloration largely as a result of mimicry. In this study we sought to discover genes involved in this variation through studying a lab-generated mutant in bumble bee Bombus terrestris, in which the typical black coloration of the pleuron, scutellum, and first metasomal tergite is replaced by yellow, a color variant also found in sister lineages to B. terrestris. Read More

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Design of novel peptide-inhibitors against the conserved bacterial transcription terminator, Rho.

J Biol Chem 2021 Apr 9:100653. Epub 2021 Apr 9.

Laboratory of Transcription, Center for DNA Fingerprinting and Diagnostics, Inner Ring Road, Uppal, Hyderabad-39, India. Electronic address:

The transcription terminator Rho regulates many physiological processes in bacteria, such as antibiotic sensitivity, DNA repair, RNA-remodeling, etc, and hence, is a potential antimicrobial target, which is unexplored. The bacteriophage P4 capsid protein, Psu, moonlights as a natural Rho antagonist. Here, we report the design of novel peptides based on the C-terminal region of Psu using phenotypic screening methods. Read More

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Microbulbifer hainanensis sp. nov., a moderately halopilic bacterium isolated from mangrove sediment.

Antonie Van Leeuwenhoek 2021 Apr 12. Epub 2021 Apr 12.

College of Food and Pharmaceutical Sciences, Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, Ningbo, 315800, People's Republic of China.

A new bacterium was successfully isolated from a mangrove sediment sample in Haikou City, Hainan Province, China. The organism is a Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, named NBU-8HK146. Strain NBU-8HK146 was able to grow at temperatures of 10-40 °C, at salinities of 0-11% (w/v) and at pH 5. Read More

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Rouxiella aceris sp. nov., isolated from tree sap and the emended description of the genus Rouxiella.

Antonie Van Leeuwenhoek 2021 Apr 11. Epub 2021 Apr 11.

Department of Biological Sciences and Biotechnology, Hannam University, Daejon, 34054, Republic of Korea.

Polyphasic taxonomic studies were performed for the seven strains, which were isolated from sap extracted from Acer pictum in Mt. Halla in Jeju, Republic of Korea. Cells of all the isolates were Gram-reaction-negative, facultatively anaerobic, short rods and contained the major isoprenoid quinone of Q-8, the predominant fatty acids of C and C cyclo and the major polar lipids including phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid. Read More

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A multilayered cross-species analysis of GRAS transcription factors uncovered their functional networks in plant adaptation to the environment.

J Adv Res 2021 Mar 29;29:191-205. Epub 2020 Oct 29.

Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

Introduction: Environmental stress is both a major force of natural selection and a prime factor affecting crop qualities and yields. The impact of the GRAS [gibberellic acid-insensitive (GAI), repressor of GA1-3 mutant (RGA), and scarecrow (SCR)] family on plant development and the potential to resist environmental stress needs much emphasis.

Objectives: This study aims to investigate the evolution, expansion, and adaptive mechanisms of of important representative plants during polyploidization. Read More

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Whole-genome sequence-based analysis of the Paenibacillus aquistagni strain DK1, a polyethylene-degrading bacterium isolated from landfill.

World J Microbiol Biotechnol 2021 Apr 11;37(5):80. Epub 2021 Apr 11.

Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N. Monte Alegre, Ribeirão Preto, SP, 14040-903, Brazil.

Polyethylene-degrading bacteria have been emerging as a rational and safe alternative in bioremediation strategies. In this context, some Paenibacillus species produce enzymes involved in the biodegradation of pollutants. Among the enzymes involved in the biodegradation of polyethylene, the alkane hydroxylases, encoded by alkB homologous genes, play a key role in this process. Read More

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Rhizobium flavescens sp. nov., Isolated from a Chlorothalonil-Contaminated Soil.

Curr Microbiol 2021 Apr 11. Epub 2021 Apr 11.

Department of Microbiology, Key Lab of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.

A Gram-negative, facultative anaerobic, non-lagellated and rod-shaped bacterium FML-4 was isolated from a chlorothalonil-contaminated soil in Nanjing, China. Phylogenetic analyses of 16S rRNA genes revealed that the strain FML-4 shared the highest sequence similarity of 97.1% with Ciceribacter thiooxidans KCTC 52231, followed by Rhizobium rosettiformans CCM 7583 (97. Read More

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Pseudomonas anatoliensis sp. nov and Pseudomonas iridis sp. nov. isolated from fish.

Syst Appl Microbiol 2021 Mar 26;44(3):126198. Epub 2021 Mar 26.

Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain; Institut Mediterrani d'Estudis Avançats (IMEDEA, CSIC-UIB), Campus UIB, 07122 Palma de Mallorca, Spain. Electronic address:

In a study carried out between 2013 and 2018 in fish farms in Turkey, several putative novel species were isolated. The 16S rRNA nucleotide sequences of fourteen strains of Gram-negative rods, which were isolated from asymptomatic and symptomatic rainbow trouts (Onchorhynchus mykiss), placed them under the genus Pseudomonas. The similarity values of the concatenated nucleotide sequences of the rpoD, rpoB, gyrB and 16S rRNA genes clustered these isolates into the P. Read More

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The endemic Chamaecytisus albidus is nodulated by symbiovar genistearum of Bradyrhizobium in the Moroccan Maamora Forest.

Syst Appl Microbiol 2021 Mar 26;44(3):126197. Epub 2021 Mar 26.

Centre de Biotechnologies Végétale et Microbienne, Biodiversité et Environnement, Faculté des Sciences, Université Mohammed V de Rabat, 4, Avenue Ibn Battouta, Rabat, Morocco. Electronic address:

Out of 54 isolates from root nodules of the Moroccan-endemic Chamaecytisus albidus plants growing in soils from the Maamora cork oak forest, 44 isolates formed nodules when used to infect their original host plant. A phenotypic analysis showed the metabolic diversity of the strains that used different carbohydrates and amino acids as sole carbon and nitrogen sources. The isolates grew on media with pH values ranging from 6 to 8. Read More

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Phenotypic Identification and Genotypic Characterization of Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Iran.

Curr Microbiol 2021 Apr 10. Epub 2021 Apr 10.

Medical Microbiology Research Center, Qazvin University of Medical Science, Qazvin, Iran.

One of the mechanisms of Klebsiella pneumoniae and Escherichia coli resistance to β-lactam antibiotics is the production of β-lactamase enzymes. Among these are the AmpC β-lactamases, which confer resistance to a class of antibiotics. However, little is known about the AmpC β-lactamases of K. Read More

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Determination of antimicrobial resistance profiles and mechanisms of using a Raman spectroscopy-based metabolomic approach.

Appl Environ Microbiol 2021 Apr 9. Epub 2021 Apr 9.

Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

Rapid identification of antimicrobial resistance (AMR) profiles and mechanisms is critical for clinical management and drug development. However, the current AMR detection approaches take up to 48 h to obtain a result. Here we demonstrate a Raman spectroscopy-based metabolomic approach to rapidly determine the AMR profile of , a major cause of foodborne gastroenteritis worldwide. Read More

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sp. nov., isolated from charcoal.

Int J Syst Evol Microbiol 2021 Apr;71(4)

Institut für Mikrobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.

A beige-pigmented, oxidase-positive bacterial isolate, Wesi-4, isolated from charcoal in 2012, was examined in detail by applying a polyphasic taxonomic approach. Cells of the isolates were rod shaped and Gram-stain negative. Examination of the 16S rRNA gene sequence of the isolate revealed highest sequence similarities to the type strains of and (both 97. Read More

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Marivivens aquimaris sp. nov., isolated from seawater.

Arch Microbiol 2021 Apr 9. Epub 2021 Apr 9.

Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup, Jeonbuk, 56212, Republic of Korea.

A Gram-stain-negative, strictly aerobic, non-flagellated, rod-shaped bacterium, designated GSB7, was isolated from seawater collected at the Yellow Sea coast of South Korea. Catalase and oxidase activities were positive. Growth occurred at pH 6. Read More

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Inhella proteolytica sp. nov. and Inhella gelatinilytica sp. nov., two novel species of the genus Inhella isolated from aquaculture water.

Arch Microbiol 2021 Apr 8. Epub 2021 Apr 8.

Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, People's Republic of China.

The two novel bacterial strains designated 1Y17 and 4Y10 from aquaculture water were characterized using a polyphasic taxonomic approach. Phylogenetic analysis of 16S rRNA gene sequences revealed that strains 1Y17 and 4Y10 belonged to the genus Inhella and were close to Inhella crocodyli CCP-18, Inhella inkyongensis IMCC1713 and Inhella fonticola TNR-25. Strains 1Y17 and 4Y10 shared 98. Read More

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Metabolic fitness of is indispensable for functional drug efflux, ergosterol, and chitin biosynthesis.

Curr Med Mycol 2020 Sep;6(3):9-14

Amity Institute of Biotechnology, Amity University Haryana, Gurugram (Manesar)-122413, India.

Background And Purpose: The increment in fungal infections, particularly due to species, is alarming due to the emergence of multidrug resistance (MDR). Hence, the identification of novel drug targets to circumvent the problem of MDR requires immediate attention. The metabolic pathway, such as glyoxylate cycle (GC), which utilizes key enzymes (isocitrate lyase [ICL] and malate synthase [MLS]), enables to adapt under glucose-deficient conditions. Read More

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September 2020