33,084 results match your criteria accompanied reduction

Natural history of EBV replication and viral load dynamics after alemtuzumab based allogeneic stem cell transplantation.

Transplant Cell Ther 2021 May 4. Epub 2021 May 4.

Department of Haematology, UCL Cancer Institute, University College London, London, UK; Department of Haematology, University College London Hospitals NHS Foundation Trust, London, UK.

Background: Epstein-Barr virus (EBV) load monitoring post-allogeneic haematopoietic stem cell transplantation (HSCT) enables earlier detection of EBV replication and is often used as a trigger for pre-emptive therapies aiming to reduce EBV-related diseases. Our institutional strategy is to treat patients with clinical signs of EBV-related disease accompanied by a rising viral load, rather than to intervene solely based on viral load. This affords an opportunity to study the natural history of EBV replication and to assess if our strategy reduces over-treatment without compromising outcomes. Read More

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Expression and characterization of 1,4-α-glucan branching enzyme from Microvirga sp. MC18 and its application in the preparation of slowly digestible starch.

Protein Expr Purif 2021 May 4:105898. Epub 2021 May 4.

Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Nutraceuticals containing modified starch with increased content of slowly-digestible starch (SDS) may reduce the prevalence of obesity, diabetes and cardiovascular diseases due to its slow digestion rate. Enzymatic methods for the preparation of modified starch have attracted increasing attention because of their low environmental impact, safety and specificity. In this study, the efficient glucan branching enzyme McGBE from Microvirga sp. Read More

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[Diagnosis, Treatment and Complications of Knee Dislocation: a Retrospective Study].

Acta Chir Orthop Traumatol Cech 2021 ;88(2):107-116

Klinika ortopedie a traumatologie pohybového ústrojí a Lékařské fakulty Univerzity Karlovy a Fakultní nemocnice, Plzeň.

PURPOSE OF THE STUDY Acute knee dislocation is a less common injury of the knee joint. It is, however, a serious injury with a high rate of nerve and vascular damage and it is considered a limb threatening injury with long-term functional disability, which can ultimately lead to amputation. Knee dislocations constitute less than 0. Read More

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January 2021

[Simultaneous Bilateral Femoral Osteotomies in Neurogenic Hip Instability: a Feasibility Study].

Acta Chir Orthop Traumatol Cech 2021 ;88(2):95-100

Oddělení ortopedie a traumatologie, AGEL Středomoravská nemocniční a.s., Nemocnice Přerov.

PURPOSE OF THE STUDY The study aimed to evaluate the intraoperative and early postoperative response to simultaneous bilateral femoral osteotomy usually accompanied by soft tissue release of hip joints, or open reduction, capsuloplasty, pelvic osteotomy or extraarticular shelf procedure. MATERIAL AND METHODS A bilateral surgery was performed in 16 children. Twelve children suffered from (spastic) cerebral palsy and there was one case of paralytic dislocation in a patient with myelomeningocele, while the remaining patients suffered from chromosome I aberrations, Dandy-Walker syndrome and merosin-deficient muscular dystrophy. Read More

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January 2021

[Pathomorphological aspects of urethral strictures of various etiologies].

Urologiia 2021 May(2):5-13

Pirogov Russian National Research Medical University, Moscow, Russia.

Introduction: The study of the structure of strictures of various etiologies is an open and uncertain issue of modern urology.

Aim: To evaluate the morphological and immunohistochemical structure of strictures of various etiologies.

Materials And Methods: The study involved postoperative tissue of a pathologically altered urethra of 110 patients aged 23 to 74 years who underwent treatment at the University Clinic of Urology, Russian National Research Medical University. Read More

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NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus.

Hippocampus 2021 May 7. Epub 2021 May 7.

Department of Translational Neuroscience, Jing'an District Center Hospital of Shanghai, State Key Laboratory of Medical Neurobiology and MOE Frontier Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, China.

Neuron-restrictive silencing factor (NRSF) is a zinc-finger transcription factor that regulates expression of a diverse set of genes. However, NRSF function in brain development still remains elusive. In the present study, we generated NRSF-conditional knockout (NRSF-cKO) mice by hGFAP-Cre/loxp system to study the effect of NRSF deficiency on brain development. Read More

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Altered theta and beta oscillatory synchrony in a genetic mouse model of pathological anxiety.

FASEB J 2021 Jun;35(6):e21585

Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Göttingen, Germany.

While the neural circuits mediating normal, adaptive defensive behaviors have been extensively studied, substantially less is currently known about the network mechanisms by which aberrant, pathological anxiety is encoded in the brain. Here we investigate in mice how deletion of Neuroligin-2 (Nlgn2), an inhibitory synapse-specific adhesion protein that has been associated with pathological anxiety and other psychiatric disorders, alters the communication between key brain regions involved in mediating defensive behaviors. To this end, we performed multi-site simultaneous local field potential (LFP) recordings from the basolateral amygdala (BLA), centromedial amygdala (CeM), bed nucleus of the stria terminalis (BNST), prefrontal cortex (mPFC) and ventral hippocampus (vHPC) in an open field paradigm. Read More

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Effect of Mindfulness-Based Stress Reduction Program on Psychological Symptoms, Quality of Life, and Symptom Severity in Patients with Somatic Symptom Disorder.

Adv Biomed Res 2021 26;10. Epub 2021 Feb 26.

Department of Biostatistics and Epidemiology, Faculty of Health, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Patients with somatic symptom disorder (SSD) had a poor quality of life and suffered from depression, anxiety, and stress. Mindfulness-based stress reduction (MBSR) is a psychological treatment with remarkable effects on several psychological disorders. This study aimed to evaluate the effect of the MBSR program on psychological symptoms, quality of life, and symptom severity in patients with SSD. Read More

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February 2021

Astragaloside IV and Saponins of Rhizoma Polygonati Cure Cyclophosphamide-Induced Myelosuppression in Lung Adenocarcinoma Down-Regulating miR-142-3p.

Front Oncol 2021 20;11:630921. Epub 2021 Apr 20.

Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Our previous study revealed that Shuanghuang Shengbai granule could cure the myelosuppression induced by cyclophosphamide (CTX) in lung cancer. However, its hematopoietic effects and molecular mechanisms remain not fully understood. Therefore, this study was intended to investigate the effects and the underlying mechanisms of Astragaloside IV (AS) and saponins of rhizoma polygonati (SRP), the two main bioactive ingredients of Shuanghuang Shengbai granule, on CTX-induced myelosuppression. Read More

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Glyphosate exposure attenuates testosterone synthesis via NR1D1 inhibition of StAR expression in mouse Leydig cells.

Sci Total Environ 2021 Apr 25;785:147323. Epub 2021 Apr 25.

Northwest A&F University, Yangling 712100, Shaanxi, China; Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address:

Glyphosate is a broad-spectrum herbicide that impairs testosterone synthesis in mammals. Leydig cells (LCs), the primary producers of testosterone, demonstrate rhythmic expression of circadian clock genes both in vivo and in vitro. The nuclear receptor NR1D1 is an important clock component that constitutes the subsidiary transcriptional/translational loop in the circadian clock system. Read More

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Residual-based pattern center calibration in high-resolution electron backscatter diffraction.

Micron 2021 Apr 26;146:103081. Epub 2021 Apr 26.

School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China.

High resolution electron backscatter diffraction is an emerging technique of micro-structural characterization which can be used for local elastic strain measurement. Pattern center (PC) coordinate, an important parameter which affects accuracy of HR-EBSD, should be carefully calibrated before calculation. An integrated digital image correlation (IDIC) algorithm can extract the deformation gradient tensor and return the residual between reference and targeted images simultaneously. Read More

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Ultrasonic Percutaneous Tenotomy for Recalcitrant Lateral Elbow Tendinopathy: Clinical and Sonographic Results at 90 Months.

Am J Sports Med 2021 May 6:3635465211010158. Epub 2021 May 6.

Department of Orthopaedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.

Background: In a study from our institution, ultrasonic percutaneous tenotomy of the brevis and the common extensor tendon for recalcitrant lateral elbow tendinopathy showed excellent safety profiles, high tolerability, efficiency, sustained pain relief, functional improvement, and sonographic evidence of tissue healing in 20 patients at 3 years' follow-up.

Purpose: To explore the long-term clinical and sonographic results of ultrasonic percutaneous tenotomy of the brevis and the common extensor tendon.

Study Design: Case series; Level of evidence, 4. Read More

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Cell death of hippocampal CA1 astrocytes during early epileptogenesis.

Epilepsia 2021 May 6. Epub 2021 May 6.

Institute of Cellular Neurosciences, Medical Faculty, University of Bonn, Bonn, Germany.

Objective: Growing evidence suggests that dysfunctional astrocytes are crucial players in the development of mesial temporal lobe epilepsy (MTLE). Using a mouse model closely recapitulating key alterations of chronic human MTLE with hippocampal sclerosis, here we asked whether death of astrocytes contributes to the initiation of the disease and investigated potential underlying molecular mechanisms.

Methods: Antibody staining was combined with confocal imaging and semiquantitative real-time polymerase chain reaction analysis to identify markers of different cellular death mechanisms between 4 h and 3 days after epilepsy induction. Read More

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[Emergency treatment of dislocation of large joints].

Unfallchirurg 2021 May;124(5):391-406

Klinik für Orthopädie, Unfallchirurgie und Sporttraumatologie, Marienhaus Klinikum Hetzelstift, Stiftstraße 10, 67435, Neustadt an der Weinstraße, Deutschland.

Joint dislocations are always accompanied by rupture of the joint capsule. Depending on the forces exerted on the joint as well as individual bone quality, fractures (dislocation fractures) and injuries to ligaments occur. As blood vessels and nerves can also be damaged, reduction is an urgent measure. Read More

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Prodigiosin induced the caspase-dependent apoptosis in human chronic myelogenous leukemia K562 cell.

Res Pharm Sci 2021 Feb 30;16(1):26-34. Epub 2020 Dec 30.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, I.R. Iran.

Background And Purpose: Chronic myeloid leukemia (CML) as a myeloproliferative disease is characterized by increased cellularity of bone marrow. Implementing the latest treatment protocols is currently accompanied by serious and life-threatening side effects. There are worldwide attempts to find new effective and potent therapeutic agents with minimal side effects on CML patients. Read More

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February 2021

In vitro effects of bis(N-[4-(hydroxyphenyl)methyl]-2-pyridinemethamine)zinc perchlorate monohydrate 4 on the physiology and interaction process of Leishmania amazonensis.

Parasitol Int 2021 May 2:102376. Epub 2021 May 2.

Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Programa de Pós-Graduação em Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

Leishmaniasis is one of the most relevant neglected tropical diseases in the world, affecting 14 million people. Despite the high morbidity, mortality and socio-economic impact, few therapeutic options are available for this disease. To make matters worse, the available molecules have several limitations such as limited efficacy, high cost, side effects and increased resistance. Read More

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CpG ODN as an adjuvant arouses the vigor of B cells by relieving the negative regulation of surface TLR9 to enhance the antibody response to vaccine.

Appl Microbiol Biotechnol 2021 May 5. Epub 2021 May 5.

Department of Molecular Biology in College of Basic Medical Sciences and Institute of Pediatrics in The First Hospital of Jilin University, Jilin University, Changchun, Jilin, 130021, People's Republic of China.

The surface Toll-like receptor 9 (sTLR9) has been identified on the surface of the B cells and was presumed to be a negative regulator of B cell responses. CpG ODN, a TLR9 agonist, has been successfully used as an adjuvant of hepatitis B vaccine to enhance antibody responses. However, it is unknown whether the sTLR9 is involved in regulating the activation and maturation of B cells in the antibody responses induced by CpG ODN-adjuvanted vaccines. Read More

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GIPC2 is an endocrine-specific tumor suppressor gene for both sporadic and hereditary tumors of RET- and SDHB-, but not VHL-associated clusters of pheochromocytoma/paraganglioma.

Cell Death Dis 2021 May 4;12(5):444. Epub 2021 May 4.

Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, China.

Pheochromocytoma/paraganglioma (PPGL) is an endocrine tumor of the chromaffin cells in the adrenal medulla or the paraganglia. Currently, about 70% of PPGLs can be explained by germline or somatic mutations in several broadly expressed susceptibility genes including RET, VHL, and SDHB, while for the remaining, mainly sporadic cases, the pathogenesis is still unclear. Even for known susceptible genes, how mutations in these mostly ubiquitous genes result in tissue-specific pathogenesis remains unanswered, and why RET-mutated tumors almost always occur in the adrenal while SDHB-mutated tumors mostly occur extra-adrenal remains a mystery. Read More

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Influence of surface condition on the degradation behaviour and biocompatibility of additively manufactured WE43.

Mater Sci Eng C Mater Biol Appl 2021 May 13;124:112016. Epub 2021 Mar 13.

Meotec GmbH, Aachen, Germany.

The further development of future Magnesium based biodegradable implants must consider not only the freedom of design, but also comprise implant volume reduction, as both aspects are crucial for the development of higher functionalised implants, such as plate systems or scaffold grafts in bone replacement therapy. As conventional manufacturing methods such as turning and milling are often accompanied by limitations concerning implant design and functionality, the process of laser powder bed fusion (LPBF) specifically for Magnesium alloys was recently introduced. In addition, the control of the degradation rate remains a key aspect regarding biodegradable implants. Read More

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The value of complete remission according to positron emission tomography prior to autologous stem cell transplantation in lymphoma: a population-based study showing improved outcome.

BMC Cancer 2021 May 4;21(1):500. Epub 2021 May 4.

Division of Hematology, Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, Sweden.

Background: Chimeric antigen-receptor T-cell and bispecific antibody therapies will likely necessitate a reconsideration of the role of autologous stem-cell transplantation (ASCT) in lymphoma. Patients who are likely to profit from ASCT need to be better identified.

Methods: Here, we investigated the value of positron emission tomography/computerized tomography (PET/CT) before ASCT. Read More

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Effect of Si-Miao-Yong-An decoction on the differentiation of monocytes, macrophages, and regulatory T cells in ApoE mice.

J Ethnopharmacol 2021 May 1:114178. Epub 2021 May 1.

Department of Cardiology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China. Electronic address:

Ethnopharmacological Relevance: Si-Miao-Yong-An decoction (SMYAD) is a renowned traditional Chinese medicinal formula. SMYAD was originally recorded in the "Shi Shi Mi Lu", which was edited by medical scientist Chen Shi'duo during the Qing Dynasty. SMYAD has been traditionally used to treat thromboangiitis obliterans. Read More

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The synergistic efficacy of hydroxychloroquine with methotrexate is accompanied by increased erythrocyte mean corpuscular volume.

Rheumatology (Oxford) 2021 May 3. Epub 2021 May 3.

Centre for Rheumatology, Division of Medicine, University College London, London, UK. Rayne Institute, 5 University St, Bloomsbury, London WC1E 6JF.

Objectives: To determine whether concomitant hydroxychloroquine modulates the increase in erythrocyte mean corpuscular volume (MCV) caused by methotrexate therapy, and whether this is associated with improved clinical response in rheumatoid arthritis (RA).

Methods: A retrospective observational analysis was conducted on two independent hospital datasets of biologic-naïve, early-RA patients who started oral methotrexate. Baseline characteristics, DAS28-ESR and monthly MCV after starting methotrexate were obtained. Read More

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The effects of centralizing cancer surgery on postoperative mortality: A systematic review and meta-analysis.

J Health Serv Res Policy 2021 May 4:13558196211008942. Epub 2021 May 4.

Biostatistician, Department of Clinical Governance, Azienda Unità Sanitaria Locale - IRCCS di Reggio Emilia, Italy.

Objectives: To review the evidence of the effects of centralization of cancer surgery on postoperative mortality.

Methods: We searched Medline, Embase, Cinahl, Cochrane and Scopus (up to November 2019) for studies that (i) assessed the effects of centralization of cancer surgery policies on in-hospital or 30-day mortality, or (ii) described changes in both postoperative mortality for a surgical intervention and degree of centralization using reduction in the number of hospitals or increases in the proportion of patients undergoing cancer surgery at high volume hospitals as proxy. PRISMA guidelines were followed. Read More

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Extensive weight loss reduces glycan age by altering IgG N-glycosylation.

Int J Obes (Lond) 2021 May 3. Epub 2021 May 3.

Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.

Background: Obesity, a major global health problem, is associated with increased cardiometabolic morbidity and mortality. Protein glycosylation is a frequent posttranslational modification, highly responsive to inflammation and ageing. The prospect of biological age reduction, by changing glycosylation patterns through metabolic intervention, opens many possibilities. Read More

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The impact of social distancing on COVID-19 infections and deaths.

Trop Dis Travel Med Vaccines 2021 May 4;7(1):12. Epub 2021 May 4.

Departamento de Economia, Federal Rural University of Pernambuco, Avenida Dom Manoel de Medeiros, s/n - Dois Irmãos, Recife, PE, Brazil.

Background: To assess the impact of the social isolation index on the number of infections and deaths by COVID-19 in the state of São Paulo (Brazil).

Methods: Daily isolation data, obtained through geolocation information by mobile phone, were evaluated together with the number of daily infections and deaths by COVID-19 in the state of São Paulo. The study was conducted from February 26 to May 19, 2020. Read More

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Increased ozone pollution alongside reduced nitrogen dioxide concentrations during Vienna's first COVID-19 lockdown: Significance for air quality management.

Marlon Brancher

Environ Pollut 2021 Apr 15;284:117153. Epub 2021 Apr 15.

WG Environmental Health, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210, Vienna, Austria. Electronic address:

Background: Lockdowns amid the COVID-19 pandemic have offered a real-world opportunity to better understand air quality responses to previously unseen anthropogenic emission reductions.

Methods And Main Objective: This work examines the impact of Vienna's first lockdown on ground-level concentrations of nitrogen dioxide (NO), ozone (O) and total oxidant (O). The analysis runs over January to September 2020 and considers business as usual scenarios created with machine learning models to provide a baseline for robustly diagnosing lockdown-related air quality changes. Read More

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Vacuolar occupancy is crucial for cell elongation and growth regardless of the underlying mechanism.

Plant Signal Behav 2021 May 3:1922796. Epub 2021 May 3.

Plant Pathology, University of Kaiserslautern, Kaiserslautern, Germany.

In the physiological range, the phytohormone auxin inhibits the growth of underground tissues. In the roots of , cell size inhibition has been shown to be accompanied by auxin-mediated reduction of vacuole size. A tonoplast-localized protein family (Networked 4) with actin-binding capacity was demonstrated to modulate the compactness of the vacuole. Read More

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PRAK Promotes the Pathogen Clearance by Macrophage Through Regulating Autophagy and Inflammasome Activation.

Front Immunol 2021 16;12:618561. Epub 2021 Apr 16.

Department of Immunology, School of Basic Medical Sciences, National Health Commission (NHC) Key Laboratory of Medical Immunology, Peking University, Beijing, China.

The p38 regulated/activated protein kinase (PRAK) is a protein kinase downstream of p38MAPK. The present study investigated its function in the macrophage. Myeloid-specific deletion of resulted in a significant reduction in F4/80CD11b peritoneal macrophages with decreased expression of MHC-II and CD80. Read More

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Impact of a CD36 inhibitor on Porphyromonas gingivalis mediated atherosclerosis.

Arch Oral Biol 2021 Apr 19;126:105129. Epub 2021 Apr 19.

Department of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada. Electronic address:

Objective: To determine if AP5055 drug, an inhibitor of CD36, prevents the increase in Porphyromonas gingivalis (P. gingivalis) mediated atherosclerosis in low-density lipoprotein receptor knockout (LDLR KO) mice by targeting CD36.

Methods: Male LDLR KO mice were infected with P. Read More

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Effects of miniplate anchored Herbst appliance on skeletal, dental and masticatory structures of the craniomandibular apparatus: A finite element study.

Int Orthod 2021 Apr 28. Epub 2021 Apr 28.

Indian Institute of Technology, Department of Mechanical Engineering, 110016 New Delhi, India.

Objective: To analyze the stress distribution in the hard and soft tissue structures of craniomandibular complex during mandibular advancement with miniplate anchored rigid fixed functional appliance (FFA) using Finite Element Analysis (FEA).

Material And Methods: The virtual model consisting of all the maxillofacial bones (up to calvaria), the mandible and temporomandibular joint (TMJ) was generated using the volumetric data from pre-treatment CBCT-scan of a growing patient. The masticatory muscles, other soft tissues, Herbst appliance and plate geometry were modelled mathematically. Read More

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