13,424 results match your criteria abundant normal

Identification METTL18 as a Potential Prognosis Biomarker and Associated With Immune Infiltrates in Hepatocellular Carcinoma.

Front Oncol 2021 26;11:665192. Epub 2021 May 26.

Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

Methyltransferase-like 18 (METTL18), a METTL family member, is abundant in hepatocellular carcinoma (HCC). Studies have indicated the METTL family could regulate the progress of diverse malignancies while the role of METTL18 in HCC remains unclear. Data of HCC patients were acquired from the cancer genome atlas (TCGA) and gene expression omnibus (GEO). Read More

View Article and Full-Text PDF

Systemic Characterization of Novel Immune Cell Phenotypes in Recurrent Pregnancy Loss.

Front Immunol 2021 28;12:657552. Epub 2021 May 28.

Institute of Reproductive Health, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Recurrent pregnancy loss (RPL) is a disturbing disease in women, and 50% of RPL is reported to be associated with immune dysfunction. Most previous studies of RPL focused mainly on the relationship between RPL and either T cells or natural killer (NK) cells in peripheral blood and the decidua; few studies presented the systemic profiles of the peripheral immune cell subsets in RPL women. Herein, we simultaneously detected 63 immune cell phenotypes in the peripheral blood from nonpregnant women (NPW), women with a history of normal pregnancy (NP) and women with a history of RPL (RPL) by multi-parameter flow cytometry. Read More

View Article and Full-Text PDF

Current and Future Roles of Circular RNAs in Normal and Pathological Endometrium.

Front Endocrinol (Lausanne) 2021 26;12:668073. Epub 2021 May 26.

Department of Gynecology, Shenzhen Second People's Hospital/The First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.

The uterine endometrium, which lines the mammalian uterus, is essential for embryo implantation. This lining undergoes significant changes during sexual and menstrual cycles. The endometrium is also associated with hormone-related diseases such as endometriosis and endometrial cancer. Read More

View Article and Full-Text PDF

Recent advances in biotechnological production of polyunsaturated fatty acids by .

Crit Rev Food Sci Nutr 2021 Jun 14:1-15. Epub 2021 Jun 14.

School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, People's Republic of China.

Owing to the important physiological functions, polyunsaturated fatty acids (PUFAs) play a vital role in protecting human health, such as preventing cancer, cardiovascular disease, and diabetes. Specifically, has been identified as the most popular non-conventional oleaginous yeast, which can accumulate the abundant intracellular lipids, indicating that has great potential as an industrial host for production of PUFAs. Notably, some novel engineering strategies have been applied to endow and improve the abilities of to synthesize PUFAs, including construction and optimization of PUFAs biosynthetic pathways, improvement of preucrsors acetyl-coA and NADPH supply, inhibition of competing pathways, knockout of β-oxidation pathways, regulation of oxidative stress defense pathways, and regulation of genes involved in upstream lipid metabolism. Read More

View Article and Full-Text PDF

Disturbed craving regulation to gaming cues in internet gaming disorder: Implications for uncontrolled gaming behaviors.

J Psychiatr Res 2021 Jun 1;140:250-259. Epub 2021 Jun 1.

Center for Cognition and Brain Disorders, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang Province, China; Zhejiang Key Laboratory for Research in Assessment of Cognitive Impairments, Hangzhou, Zhejiang Province, China. Electronic address:

Background: The ability to control craving for games is very important to abstain from Internet gaming disorder (IGD) and abundant clinical evidence has suggested that craving dysregulation is the essential pathogenesis for IGD. However, the neural mechanism underlying this feature remains unclear.

Methods: Subjective evaluation and fMRI data from 44 participants (IGD participants: 21; recreational Internet game users (RGUs): 23) were collected while they were performing a regulation of craving task. Read More

View Article and Full-Text PDF

CoS nanowires grown on TiCT are promising electrodes for supercapacitors: High capacitance and remarkable cycle capability.

J Colloid Interface Sci 2021 Jun 4;602:123-130. Epub 2021 Jun 4.

Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, School of Physics and Electronic Engineering, Harbin Normal University, Harbin 150025, People's Republic of China. Electronic address:

Benefitting from the large interlayer spacing, ultrahigh conductivity and abundant surface chemistry, TiCT has been a promising electrode material for supercapacitors (SCs). CoS has attracted much attention due to its low cost, weak Co-S bond and relatively high theoretical capacity. Herein, CoS nanowires were grown on few-layered TiCT by one-step solvothermal method as a SC electrode. Read More

View Article and Full-Text PDF

Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions.

Plant Cell 2021 Jun 12. Epub 2021 Jun 12.

School of Life Sciences & Institute of Plant and Food Science & Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.

DNA methylation in the non-CG context is widespread in the plant kingdom and abundant in mammalian tissues such as the brain and pluripotent cells. Non-CG methylation in Arabidopsis thaliana is coordinately regulated by DOMAINS REARRANGED METHYLTRANSFERASE (DRM) and CHROMOMETHYLASE (CMT) proteins but has yet to be systematically studied in major crops due to difficulties in obtaining genetic materials. Here, utilizing the highly efficient multiplex CRISPR-Cas9 genome-editing system, we created single- and multiple-knockout mutants for all nine DNA methyltransferases in rice (Oryza sativa) and profiled their whole-genome methylation status at single-nucleotide resolution. Read More

View Article and Full-Text PDF

ZIF-based boronic acid functionalized metal-organic frameworks for the enrichment of cis-diol-containing luteolin from food samples prior to  HPLC.

Mikrochim Acta 2021 Jun 11;188(7):229. Epub 2021 Jun 11.

Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Heilongjiang Province, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, 150025, People's Republic of China.

Zeolite imidazole framework-based boronic acid-functionalized metal-organic frameworks ([email protected]@BA-Zr-MOFs) were developed as an adsorbent for solid phase extraction (SPE) of luteolin (LTL) from peanut shell samples. Herein, ZIF-67 as a support matrix, polydopamine (PDA) as a coating to introduce amino and hydroxyl groups on the matrix surface to fix metal-organic frameworks (MOFs), zirconium tetrachloride (ZrCl) as a precursor, terephthalic acid (TPA), and 3-carboxyphenylboronic acid (3-CPBA) as the mutual organic building blocks, and 3-CPBA was also a boronate affinity functional monomer. The effects of synthesis conditions, SPE conditions, selectivity, competitivity, reproducibility, and reusability were evaluated in detail. Read More

View Article and Full-Text PDF

Chemoenzymatic modular assembly of O-GalNAc glycans for functional glycomics.

Nat Commun 2021 06 11;12(1):3573. Epub 2021 Jun 11.

Department of Chemistry, Georgia State University, Atlanta, GA, 30303, USA.

O-GalNAc glycans (or mucin O-glycans) play pivotal roles in diverse biological and pathological processes, including tumor growth and progression. Structurally defined O-GalNAc glycans are essential for functional studies but synthetic challenges and their inherent structural diversity and complexity have limited access to these compounds. Herein, we report an efficient and robust chemoenzymatic modular assembly (CEMA) strategy to construct structurally diverse O-GalNAc glycans. Read More

View Article and Full-Text PDF

Distribution of complete blood count constituents in gestational diabetes mellitus.

Medicine (Baltimore) 2021 Jun;100(23):e26301

Department of Obstetrics and Gynecology, Shenzhen Longhua District Central Hospital, Guangdong Medical University, Shenzhen, Guangdong, China.

Background Trial Design: The incidence rate of gestational diabetes is high. In the long run, it harms the health of both the mother and child. In order to understand the distribution of hematological cells with gestational diabetes mellitus (GDM), a longitudinal cohort study was conducted from 2012 to 2018. Read More

View Article and Full-Text PDF

BRCA1 binds TERRA RNA and suppresses R-Loop-based telomeric DNA damage.

Nat Commun 2021 06 10;12(1):3542. Epub 2021 Jun 10.

Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.

R-loop structures act as modulators of physiological processes such as transcription termination, gene regulation, and DNA repair. However, they can cause transcription-replication conflicts and give rise to genomic instability, particularly at telomeres, which are prone to forming DNA secondary structures. Here, we demonstrate that BRCA1 binds TERRA RNA, directly and physically via its N-terminal nuclear localization sequence, as well as telomere-specific shelterin proteins in an R-loop-, and a cell cycle-dependent manner. Read More

View Article and Full-Text PDF

Interannual variation in riparian vegetation cover and its relationship with river flow under a high level of human intervention: an example from the Yongding River Basin.

Environ Monit Assess 2021 Jun 10;193(7):406. Epub 2021 Jun 10.

College of Water Sciences, Beijing Normal University, Beijing, 100875, China.

Riparian vegetation cover is significantly affected by a river's hydrological conditions. Especially in arid and semiarid areas, low flow will degrade riparian vegetation, and recent, intensive human activities in the Yongding River Basin have caused a sharp decrease in river flow. We analyzed interannual change in riparian vegetation, river flow effects, and land use on vegetation coverage using the 40 years (1977-2016) of remote sensing images and river flow, combined with 38 years (1980-2018) of land use data. Read More

View Article and Full-Text PDF

Consuming Different Structural Parts of Bamboo Induce Gut Microbiome Changes in Captive Giant Pandas.

Curr Microbiol 2021 Jun 9. Epub 2021 Jun 9.

Chengdu Research Base of Giant Panda Breeding, Chengdu, 610081, China.

Giant pandas consume different structural parts of bamboo (shoots, leaves and culms) during different seasons. Previous research showed different bamboo parts have varying nutritional content and that a long-term diet consisting of a single part of bamboo resulted in remarkable metabolic changes within captive giant pandas. However, the effects on the gut microbiome of giant pandas, as a result of a single bamboo part diet, have not been investigated. Read More

View Article and Full-Text PDF

Argonaute Binding within Human Nuclear RNA and its Impact on Alternative Splicing.

RNA 2021 Jun 9. Epub 2021 Jun 9.


Mammalian RNA interference (RNAi) is often linked to the regulation of gene expression in the cytoplasm. Synthetic RNAs, however, can also act through the RNAi pathway to regulate transcription and splicing. While nuclear regulation by synthetic RNAs can be robust, a critical unanswered question is whether endogenous functions for nuclear RNAi exist in mammalian cells. Read More

View Article and Full-Text PDF

Association between colorectal cancer and Fusobacterium nucleatum and Bacteroides fragilis bacteria in Iranian patients: a preliminary study.

Infect Agent Cancer 2021 Jun 9;16(1):41. Epub 2021 Jun 9.

Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Background And Aim: Recent studies have proposed that commensal bacteria might be involved in the development and progression of gastrointestinal disorders such as colorectal cancer (CRC). Therefore, in this study, the relative abundance of Fusobacterium nucleatum, Bacteroides fragilis, Streptococcus bovis/gallolyticus, and Enteropathogenic Escherichia coli (EPEC) in CRC tissues, and their association with clinicopathologic characteristics of CRC was investigated in Iranian patients. Moreover, the role of these bacteria in the CRC-associated mutations including PIK3CA, KRAS, and BRAF was studied. Read More

View Article and Full-Text PDF

Gut microbiota of adults with different metabolic phenotypes.

Nutrition 2021 Apr 28;90:111293. Epub 2021 Apr 28.

Postgraduate Program in Medicine (Cardiology), Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Electronic address:

Objective: The aim of this study was to investigate the gut microbiota of individuals with different metabolic phenotypes and to compare their characteristics.

Methods: This was a cross-sectional study with 109 adults, classified according to metabolic status, by body mass index, and homeostasis model assessment. Anthropometric and biochemical characteristics and blood pressure were evaluated. Read More

View Article and Full-Text PDF

Asporin inhibits collagen matrix-mediated intercellular mechanocommunications between fibroblasts during keloid progression.

FASEB J 2021 Jul;35(7):e21705

Department of Dermatology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.

Keloids are fibrotic lesions that grow unceasingly and invasively and are driven by local mechanical stimuli. Unlike other fibrotic diseases and normal wound healing, keloids exhibit little transformation of dermal fibroblasts into α-SMA myofibroblasts. This study showed that asporin is the most strongly expressed gene in keloids and its gene-ontology terms relate strongly to ECM metabolism/organization. Read More

View Article and Full-Text PDF

miR-361-5p as a promising qRT-PCR internal control for tumor and normal breast tissues.

PLoS One 2021 8;16(6):e0253009. Epub 2021 Jun 8.

Molecular Genetics Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran.

Background: One of the most widely used evaluation methods in miRNA experiments is qRT-PCR. However, selecting suitable internal controls (IC) is crucial for qRT-PCR experiments. Currently, there is no consensus on the ICs for miRNA qRT-PCR experiments in breast cancer. Read More

View Article and Full-Text PDF

[Hypoxia Accelerate β-Actin Expression through Transcriptional Activation of ACTB by Nuclear Respiratory Factor-1].

Mol Biol (Mosk) 2021 May-Jun;55(3):460-467

Institute of Special Environmental Medicine, Nantong University, 226019 China.

Cytoskeletal protein β-actin is abundant both in the cytoplasm and the nucleus, its mRNA is commonly utilized an internal control for gene expression analysis. Recent reports demostrated that hypoxia influences the levels of β-actin in a variety of cells. The mechanism underlying this change are not yet elucidated. Read More

View Article and Full-Text PDF

Au-to-Ag coordinate-covalent bonding and other supramolecular interactions with covalent bonding strength.

Chem Sci 2020 Sep 11;11(41):11179-11188. Epub 2020 Sep 11.

Department of Chemistry, University of North Texas 1155 Union Circle #305070 Denton Texas 76203 USA

An efficient strategy for designing charge-transfer complexes using coinage metal cyclic trinuclear complexes (CTCs) is described herein. Due to opposite quadrupolar electrostatic contributions from metal ions and ligand substituents, [Au(μ-Pz-(i-CH))]·[Ag(μ-Tz-(-CF))] (Pz = pyrazolate, Tz = triazolate) has been obtained and its structure verified by single crystal X-ray diffraction - representing the 1 crystallographically-verified stacked adduct of monovalent coinage metal CTCs. Abundant supramolecular interactions with aggregate covalent bonding strength arise from a combination of M-M' (Au → Ag), metal-π, π-π interactions and hydrogen bonding in this charge-transfer complex, according to density functional theory analyses, yielding a computed binding energy of 66 kcal mol between the two trimer moieties - a large value for intermolecular interactions between adjacent d centres (nearly doubling the value for a recently-claimed Au(i) → Cu(i) polar-covalent bond: , 2017, , E5042) - which becomes 87 kcal mol with benzene stacking. Read More

View Article and Full-Text PDF
September 2020

A resected case of focal autoimmune pancreatitis with pancreatic duct wall thickening representing periductal lymphoplasmacytic infiltrate.

Clin J Gastroenterol 2021 Jun 6. Epub 2021 Jun 6.

Department of Gastroenterology, Saku Central Hospital Advanced Care Center, #106 Monteseresso 3671-46, Nakagomi, Saku-shi, Nagano, 385-0051, Japan.

This case revealed that branch pancreatic duct wall thickening by endoscopic ultrasonography represented periductal lymphoplasmacytic infiltrate as a characteristic histopathological finding of autoimmune pancreatitis, which may help in the diagnosis of autoimmune pancreatitis. An 80-year-old man was referred because fluorodeoxyglucose-positron emission tomography for lung cancer indicated abnormal uptake in the pancreatic head. Computed tomography showed an enhanced mass with cystic structures in the pancreatic head. Read More

View Article and Full-Text PDF

SnoRNA copy regulation affects family size, genomic location and family abundance levels.

BMC Genomics 2021 Jun 5;22(1):414. Epub 2021 Jun 5.

Département de biochimie et de génomique fonctionnelle, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec, J1E 4K8, Canada.

Background: Small nucleolar RNAs (snoRNAs) are an abundant class of noncoding RNAs present in all eukaryotes and best known for their involvement in ribosome biogenesis. In mammalian genomes, many snoRNAs exist in multiple copies, resulting from recombination and retrotransposition from an ancestral snoRNA. To gain insight into snoRNA copy regulation, we used Rfam classification and normal human tissue expression datasets generated using low structure bias RNA-seq to characterize snoRNA families. Read More

View Article and Full-Text PDF

TSHZ3 functions as a tumor suppressor by DNA methylation in colorectal cancer.

Clin Res Hepatol Gastroenterol 2021 Jun 2:101725. Epub 2021 Jun 2.

Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China. Electronic address:

Objective: Teashirt zinc finger homeobox 3 (TSHZ3) is currently reported to be aberrantly expressed in several tumors, but the detailed functions and epigenetic mechanisms of TSHZ3 in colorectal cancer (CRC) remain unclear.

Materials And Methods: In this study, the TSHZ3 expression in 118 CRC and normal adjacent tissues (NATs) was evaluated, and the methylation status of the TSZH3 promoter region in CRC tissues and cell lines was also analyzed.

Results: The results of PCR analysis showed that TSHZ3 was significantly down-regulated in CRC tissues, and patients with low TSHZ3 levels had a poorer 5-year overall survival (OS) rate. Read More

View Article and Full-Text PDF

Structural dependent Cr(VI) adsorption and reduction of biochar: hydrochar versus pyrochar.

Sci Total Environ 2021 Aug 14;783:147084. Epub 2021 Apr 14.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Institute of Environmental & Applied Chemistry, College of Chemistry, Central China Normal University, Wuhan 430079, People's Republic of China. Electronic address:

Hydrochar and pyrochar are two typical biochars, and possess different intrinsic structures and chemical properties as well as pollutant removal abilities. However, their structural dependent pollutant removal performances and the related mechanisms are far less studied. In this study, we systematically compared the Cr(VI) removal processes of hydrochar and pyrochar in dark and under simulated sunlight at pH 5. Read More

View Article and Full-Text PDF

Simultaneous enhancement of power generation and chlorophenol degradation in nonmodified microbial fuel cells using an electroactive biofilm carbon felt anode.

Sci Total Environ 2021 Aug 19;783:147045. Epub 2021 Apr 19.

School of Environment, Northeast Normal University, Changchun 130117, PR China. Electronic address:

Microbial fuel cells (MFCs) are an emerging technique presenting remarkable potential. In the current MFC, an electroactive biofilm anode was inoculated with activated sludge from a local municipal sewage treatment plant. The output voltage peaked at 0. Read More

View Article and Full-Text PDF

Conjugated microporous polyarylimides immobilization on carbon nanotubes with improved utilization of carbonyls as cathode materials for lithium/sodium-ion batteries.

J Colloid Interface Sci 2021 May 19;601:446-453. Epub 2021 May 19.

School of Materials Science and Engineering, Changchun University of Science and Technology, Changchun 130022, China; Key Laboratory of Polyoxometalate Science of the Ministry of Education and Faculty of Chemistry, Northeast Normal University, 5628 Renmin Street, Changchun 130024, PR China. Electronic address:

Aromatic polyimide (PI)-based compounds have been widely studied for lithium-ion batteries (LIBs) and sodium-ion batteries (SIBs) due to their higher specific energy density, economical, environmentally friendly and adjustable redox potential window. However, their solubility in aprotic electrolytes, inherently poor conductivity and low active site utilization limit their application in large-scale energy storage system (ESS). Here, we synthesized two aromatic PI-based conjugated microporous polymers (CMPs) and integrated them with multi-walled carbon nanotubes (CNT) ([email protected] and [email protected]) for using as cathode materials for LIBs and SIBs. Read More

View Article and Full-Text PDF

Human sperm functioning is related to the aquaporin-mediated water and hydrogen peroxide transport regulation.

Biochimie 2021 Jun 2. Epub 2021 Jun 2.

Department of Molecular Medicine, Human Physiology Unit, University of Pavia, Pavia, I-27100, Italy. Electronic address:

Aquaporins (AQPs) are transmembrane water channels and some of them are permeable in addition to water to other small solutes including hydrogen peroxide. The sperm cells of mammals and fishes express different AQPs, although there is no agreement in the literature on their localization. In humans, AQP3 and AQP11 are expressed mainly in the tail, AQP7 in the head and AQP8 in the midpiece. Read More

View Article and Full-Text PDF

Comparative analysis of mite genomes reveals positive selection for diet adaptation.

Commun Biol 2021 Jun 3;4(1):668. Epub 2021 Jun 3.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Diet is a powerful evolutionary force for species adaptation and diversification. Acari is one of the most abundant clades of Arachnida, exhibiting diverse dietary types, while the underlying genetic adaptive mechanisms are not fully understood. Based on comparative analyses of 15 Acari genomes, we found genetic bases for three specialized diets. Read More

View Article and Full-Text PDF

Effects of human collagen α-1 type I-derived proteins on collagen synthesis and elastin production in human dermal fibroblasts.

BMB Rep 2021 Jun 3. Epub 2021 Jun 3.

Department of Biomedical Sciences, College of Medicine, Inha University; Program in Biomedical Science & Engineering, Inha University, Incheon 22212, Korea.

Collagen type I is the most abundant form of collagen in human tissues, and is composed of two identical α-1 type I chains and an α-2 type I chain organized in a triple helical structure. A previous study has shown that human collagen α-2 type I (hCOL1A2) promotes collagen synthesis, wound healing, and elastin production in normal human dermal fibroblasts (HDFs). However, the biological effects of human collagen α-1 type I (hCOL1A1) on various skin properties have not been investigated. Read More

View Article and Full-Text PDF

CSPG4 is a Potential Therapeutic Target in Anaplastic Thyroid Cancer.

Thyroid 2021 Jun 3. Epub 2021 Jun 3.

Weill Cornell Medicine, 12295, Surgery, New York, New York, United States;

Background: Anaplastic thyroid cancer (ATC) is a rare cancer with poor prognosis and few treatment options. The objective of this study was to investigate new immune-associated therapeutic targets by identifying ATC-derived, human leukocyte antigen (HLA) class II-presenting peptides. One protein that generated multiple peptides in ATC was chondroitin sulfate -proteoglycan-4 (CSPG4), a transmembrane proteoglycan with increased expression in multiple aggressive cancers but not yet investigated in ATC. Read More

View Article and Full-Text PDF