7,158 results match your criteria absolute quantification


Appraising regional anthropogenic heat flux using high spatial resolution NTL and POI data: A case study in the Beijing-Tianjin-Hebei region, China.

Environ Pollut 2021 Oct 11;292(Pt A):118359. Epub 2021 Oct 11.

State Key Laboratory of Remote Sensing Science, Faculty of Geographical Science, Beijing Normal University, Beijing, 100875, China.

Rapid urbanization and the aggregation of human activities in cities have resulted in large amounts of anthropogenic heat (AH) emission, which affects urban climate. Quantifying and assessing the AH emission values accurately and analyzing their spatial distribution characteristics is important to understand the energy exchange processes of urban areas. In this study, the high spatial resolution anthropogenic heat flux (AHF) quantification and spatial distribution analysis were conducted using multi-source data in the Beijing-Tianjin-Hebei region (BTH region) of China. Read More

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October 2021

TSSK4 upregulation in alveolar epithelial type-II cells facilitates pulmonary fibrosis through HSP90-AKT signaling restriction and AT-II apoptosis.

Cell Death Dis 2021 Oct 13;12(10):938. Epub 2021 Oct 13.

The Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allery & Clinical Immunology, Guangzhou Medical University, Guangzhou, Guangdong, 510260, China.

Alveolar epithelial injury is one of the important pathological changes in idiopathic pulmonary interstitial fibrosis (IPF), but the regulatory mechanism remains unclear. Here, we reported that alveolar epithelial type-II cells (AT II) play important roles in pathological process of pulmonary fibrosis. Through iTRAQ (isobaric tagging for relative and absolute quantification) quantitative proteomics, TSSK4 was identified to be upregulated in bleomycin-induced fibrotic mice model, which was further confirmed in clinical IPF patients' tissue specimens. Read More

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October 2021

Yeast-based reference materials for quantitative metabolomics.

Anal Bioanal Chem 2021 Oct 13. Epub 2021 Oct 13.

Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Waehringer Str. 38, 1090, Vienna, Austria.

We introduce a new concept of yeast-derived biological matrix reference material for metabolomics research relying on in vivo synthesis of a defined biomass, standardized extraction followed by absolute quantification with isotope dilution. The yeast Pichia pastoris was grown using full control- and online monitoring fed-batch fermentations followed by fast cold methanol quenching and boiling ethanol extraction. Dried extracts served for the quantification campaign. Read More

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October 2021

Defining matrix Gla protein expression in the Dunkin-Hartley guinea pig model of spontaneous osteoarthritis.

BMC Musculoskelet Disord 2021 Oct 12;22(1):870. Epub 2021 Oct 12.

Department of Rehabilitation, Shengjing Hospital of China Medical University, No.16, Puhe Street, Shenyang North New Area, Shenyang, 110134, Liaoning Province, China.

Background: Matrix Gla (γ-carboxyglutamate) protein (MGP) is considered a strong inhibitor of ectopic calcification, and it has been associated with OA severity, although not conclusively. We utilized male Dunkin-Hartley (DH) guinea pigs to investigate the expression of MGP throughout aging and disease pathogenesis in a spontaneous model.

Method: Twenty-five male DH guinea pigs were obtained and nurtured to several timepoints, and then randomly and equally divided by age into five subgroups (1-, 3-, 6-, 9-, and 12-months, with the 1-month group as the reference group). Read More

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October 2021

Characterization and Identification of Bioactive Polyphenols in the Roxb. Pericarp Extract.

Molecules 2021 Sep 24;26(19). Epub 2021 Sep 24.

Department of Nutritional Science, Faculty of Health and Welfare Sciences, Okayama Prefectural University, Okayama 719-1197, Japan.

In this study, we present the isolation and characterization of the structure of six gallotannins (-), three ellagitannins (-), a neolignan glucoside (), and three related polyphenolic compounds (gallic acid, and ) from Roxb. pericarp extract (TBE). Among the isolates, the structure of compound possessing a previously unclear absolute configuration was unambiguously determined through nuclear magnetic resonance and circular dichroism analyses. Read More

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September 2021

Co-Density Distribution Maps for Advanced Molecule Colocalization and Co-Distribution Analysis.

Sensors (Basel) 2021 Sep 24;21(19). Epub 2021 Sep 24.

Advanced Research Center on Electronic Systems (ARCES) for Information and Communication Technologies "E. De Castro", Alma Mater Studiorum-University of Bologna, I-40125 Bologna, Italy.

Cellular and subcellular spatial colocalization of structures and molecules in biological specimens is an important indicator of their co-compartmentalization and interaction. Presently, colocalization in biomedical images is addressed with visual inspection and quantified by co-occurrence and correlation coefficients. However, such measures alone cannot capture the complexity of the interactions, which does not limit itself to signal intensity. Read More

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September 2021

High-accuracy quantitative principle of a new compact digital PCR equipment: Lab On An Array.

Genomics Inform 2021 Sep 30;19(3):e34. Epub 2021 Sep 30.

Department of Bioconvergence Engineering, Dankook University, Yongin 16890, Korea.

Digital PCR (dPCR) is the third-generation PCR that enables real-time absolute quantification without reference materials. Recently, global diagnosis companies have developed new dPCR equipment. In line with the development, the Lab On An Array (LOAA) dPCR analyzer (Optolane) was launched last year. Read More

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September 2021

Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout.

Proteome Sci 2021 Oct 11;19(1):12. Epub 2021 Oct 11.

Center for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, PR China.

Background: Gout is a common and complex form of immunoreactive arthritis based on hyperuricemia, while the symptoms would turn to remission or even got worse. So, it is hard to early identify whether an asymptomatic hyperuricemia (AHU) patient will be susceptible to get acute gout attack and it is also hard to predict the process of gout remission to flare. Here, we report that the plasma proteins profile can distinguish among acute gout (AG), remission of gout (RG), AHU patients, and healthy controls. Read More

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October 2021

[Screening Serum Differential Proteins in Children with Acute Promyelocytic Leukemia Based on iTRAQ Technique].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Oct;29(5):1462-1470

Institute of Hematology, Henan Provincial People's Hospital, Henan Key Laboratory of Stem Cell Differentiation and Modification; Department of Hematology, Henan Provincial People's Hospital; Zhengzhou 450003, Henan Province, China,E-mail:

Objective: To screen the serum differentially expressed proteins of APL in children.

Methods: Serum protein expression profiles from 20 cases of normal healthy controls, and 20 cases of APL patients were detected by iTRAQ (isobaric tag for relative and absolute quantification)labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry(2DLC-MS/MS), and analyzed by bioinformatics software. S100A8, LRG1 and SPARC were validated by ELISA. Read More

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October 2021

Benzalkonium chlorides (C12) inhibits growth but motivates microcystins release of Microcystis aeruginosa revealed by morphological, physiological, and iTRAQ investigation.

Environ Pollut 2021 Oct 6;292(Pt A):118305. Epub 2021 Oct 6.

National Joint Local Engineering Research Center for Shale Gas Exploration and Development, Chongqing Institute of Geology and Mineral Resources, Chongqing, 401120, China.

Due to the large-scale outbreak of Corona Virus Disease (2019), amounts of disinfecting agents was regularly used in public environments and their potential toxicity towards organisms needed to be appreciated. Thus, one mostly used cationic disinfectant, benzalkonium chlorides (BAC(C12)), was selected to assess its potential toxicity one common cyanobacteria Microcystis aeruginosa (M. aeruginosa) in this study. Read More

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October 2021

Age-specific burden of cervical cancer associated with HIV: a global analysis with a focus on sub-Saharan Africa.

Int J Cancer 2021 Oct 9. Epub 2021 Oct 9.

Department of Global HIV, Hepatitis and STIs Programmes, World Health Organization, Geneva, Switzerland.

HIV substantially worsens human papillomavirus (HPV) carcinogenicity and contributes to an important population excess of cervical cancer, particularly in sub-Saharan Africa (SSA). We estimated HIV- and age-stratified cervical cancer burden at a country, regional, and global level in 2020. Proportions of cervical cancer a) diagnosed in women living with HIV (WLHIV), and b) attributable to HIV, were calculated using age-specific estimates of HIV prevalence (UNAIDS) and relative risk. Read More

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October 2021

Multiple heart-cutting two dimensional liquid chromatography and isotope dilution tandem mass spectrometry for the absolute quantification of proteins in human serum.

Anal Chim Acta 2021 Nov 3;1184:339022. Epub 2021 Sep 3.

Department of Physical and Analytical Chemistry, University of Oviedo, Calle Julián Clavería 8, 33006, Oviedo, Spain.

We evaluate here the combination of two-dimensional liquid chromatography (2D-LC) in the multiple heart cutting mode and isotope dilution tandem mass spectrometry for the direct analysis of tryptic digests of serum samples. As a proof of concept, we attempt the quantification of proteotypic peptides of Apolipoprotein AIV (APOA4), Complement C3 (C3) and Vitronectin (VTN) which have been previously identified as potential candidate biomarkers of glaucoma. Using this 2D-LC strategy, analyte enrichment steps are avoided and the sample preparation involved after enzymatic digestion amounted to a simple centrifugation, evaporation of the supernatant and reconstitution in the 1D mobile phase. Read More

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November 2021

Absolute quantification of senescence mediators in cells using multiple reaction monitoring liquid chromatography-Tandem mass spectrometry.

Anal Chim Acta 2021 Nov 2;1184:339009. Epub 2021 Sep 2.

Department of Biochemistry and Molecular Medicine, College of Medicine, Alfaisal University, Riyadh, 11533, Saudi Arabia. Electronic address:

Background: The identification of unique senescence markers remains challenging. Current hallmarks of senescent cells, including increased senescence-associated β-galactosidase activity, increased levels of cell cycle regulators such as p16, p27, and p53, and altered levels of sirtuins and lamins, are detected commonly by Western blot and immunohistochemistry methods. Mass spectrometry outperforms these conventional quantification methods in terms of high throughput, specificity, and reproducibility. Read More

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November 2021

A simultaneous identification and quantification strategy for determination of sulfhydryl-containing metabolites in normal- and high-fat diet hamsters using stable isotope labeling combined with LC-MS.

Anal Chim Acta 2021 Nov 1;1184:339016. Epub 2021 Sep 1.

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, PR China; School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310058, PR China. Electronic address:

Sulfur-containing metabolites are related to several physiologic disorders and metabolic diseases. In this study, a simultaneous identification and quantification strategy in one batch for determination of sulfhydryl-containing metabolites was developed using stable isotope labeling combined with liquid chromatography-tandem mass spectrometry (SIL-LC-MS). In the proposed method, a pair of isotope labeling reagents, D/D-N-ethylmaleimide (D/D-NEM), was used to derivatize sulfhydryl-containing metabolites in blood and plasma of normal- and high-fat-diet (NFD and HFD) hamsters for reduced (-SH) and total (-SH, -S-S-, S-glutathionylated proteins) analysis. Read More

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November 2021

Uncertainty-aware body composition analysis with deep regression ensembles on UK Biobank MRI.

Comput Med Imaging Graph 2021 Sep 23;93:101994. Epub 2021 Sep 23.

Uppsala University, Department of Surgical Sciences, Uppsala, Sweden; Antaros Medical AB, BioVenture Hub, Mölndal, Sweden.

Along with rich health-related metadata, medical images have been acquired for over 40,000 male and female UK Biobank participants, aged 44-82, since 2014. Phenotypes derived from these images, such as measurements of body composition from MRI, can reveal new links between genetics, cardiovascular disease, and metabolic conditions. In this work, six measurements of body composition and adipose tissues were automatically estimated by image-based, deep regression with ResNet50 neural networks from neck-to-knee body MRI. Read More

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September 2021

Small-angle X-ray scattering to quantify the incorporation and analyze the disposition of magnetic nanoparticles inside cells.

J Colloid Interface Sci 2021 Sep 29;608(Pt 1):1-12. Epub 2021 Sep 29.

Instituto de Física La Plata (IFLP- CONICET), Departamento de Física, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), c.c. 67, 1900 La Plata, Argentina. Electronic address:

Access to detailed information on cells loaded with nanoparticles with nanoscale precision is of a long-standing interest in many areas of nanomedicine. In this context, designing a single experiment able to provide statistical mean data from a large number of living unsectioned cells concerning information on the nanoparticle size and aggregation inside cell endosomes and accurate nanoparticle cell up-take is of paramount importance. Small-angle X-ray scattering (SAXS) is presented here as a tool to achieve such relevant data. Read More

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September 2021

AI-based radiation dose quantification for estimation of heart disease risk in breast cancer survivors after radiotherapy.

Int J Radiat Oncol Biol Phys 2021 Oct 5. Epub 2021 Oct 5.

Image Sciences Institute, University Medical Center Utrecht, Utrecht, the Netherlands; Department of Biomedical Engineering and Physics, Amsterdam University Medical Centers - Location AMC, University of Amsterdam, Amsterdam, the Netherlands; Department of Radiology and Nuclear Medicine, Amsterdam University Medical Centers - Location AMC, University of Amsterdam, Amsterdam, the Netherlands; Amsterdam Cardiovascular Sciences, Amsterdam UMC - location AMC.

Purpose: To investigate whether dose planned to cardiac structures is associated with the risk of heart disease (HD) in breast cancer patients treated with radiotherapy, and whether this association is modified by presence of coronary artery calcification (CAC).

Materials And Methods: Radiotherapy planning CT scans and corresponding dose distribution maps of 5,561 patients were collected, 5300 patients remained after exclusion of ineligible patients and duplicates. 1,899 patients received their CT scan before 2011, allowing long follow-up. Read More

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October 2021

Transcriptomic evidences for microbial carbon and nitrogen cycles in the deoxygenated seawaters of Bohai Sea.

Environ Int 2021 Oct 4;158:106889. Epub 2021 Oct 4.

State Key Laboratory of Marine Environmental Science, Fujian Key Laboratory of Marine Carbon Sequestration, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, Fujian, PR China. Electronic address:

Eutrophication-induced water deoxygenation occurs continually in coastal oceans, and alters community structure, metabolic processes, and the energy shunt, resulting in a major threat to the ecological environment. Seasonal deoxygenation events have occurred in the Bohai Sea (China), however, how these affect the functional activity of microorganisms remains unclear. Here, through the use of absolute quantification of 16S rRNA genes amplicon sequencing and metatranscriptomics approaches, we investigated the structure of the microbial community and the patterns of transcriptional activity in deoxygenated seawaters. Read More

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October 2021

Automated Morphometric Analysis of the Hip Joint on MRI from the German National Cohort Study.

Radiol Artif Intell 2021 Sep 2;3(5):e200213. Epub 2021 Jun 2.

Institute of Signal Processing and Systems Theory, University of Stuttgart, Pfaffenwaldring 47, 70550 Stuttgart, Germany (M.F., M.Z., B.Y.); Department of Diagnostic and Interventional Radiology, Section on Experimental Radiology, University Hospital Tübingen, Tübingen, Germany (S.S.W., T.H., M.N., F.S.); and Empirical Inference Department, Max Planck Institute for Intelligent Systems, Tübingen, Germany (T.H.).

Purpose: To develop and validate an automated morphometric analysis framework for the quantitative analysis of geometric hip joint parameters in MR images from the German National Cohort (GNC) study.

Materials And Methods: A secondary analysis on 40 participants (mean age, 51 years; age range, 30-67 years; 25 women) from the prospective GNC MRI study (2015-2016) was performed. Based on a proton density-weighted three-dimensional fast spin-echo sequence, a morphometric analysis approach was developed, including deep learning-based landmark localization, bone segmentation of the femora and pelvis, and a shape model for annotation transfer. Read More

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September 2021

Target-Triggered Nanomaterial Self-Assembly Induced Electromagnetic Hot-Spot Generation for SERS-Fluorescence Dual-Mode In Situ Monitoring MiRNA-Guided Phototherapy.

Anal Chem 2021 Oct 5. Epub 2021 Oct 5.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Jiangsu 221004, Xuzhou, China.

A multifunctional theranostic nanosystem that integrates dynamic monitoring and therapeutic functions is necessary for precision tumor medicine. Herein, an entropy-driven self-assembly nanomachine is developed that overcomes the mechanism differences of different diagnostic modes and is applied to miRNA surface-enhanced Raman scattering (SERS)-fluorescence dual-mode dynamic monitoring and synergy phototherapy. It is worth noting that the activated dual-mode theranostic nanosystem (DTN) is capable of tumor in situ fluorescence imaging and SERS absolute quantification of the target. Read More

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October 2021

Hierarchical Censored Bayesian Analysis of Visual Field Progression.

Transl Vis Sci Technol 2021 Oct;10(12)

City, University of London, Optometry and Visual Sciences, London, UK.

Purpose: To develop a Bayesian model (BM) for visual field (VF) progression accounting for the hierarchical, censored and heteroskedastic nature of the data.

Methods: Three versions of a hierarchical BM were developed: a simple linear (Hi-linear); censored at 0 dB (Hi-censored); heteroskedastic censored (Hi-HSK). For the latter, we modeled the test variability according to VF sensitivity using a large test-retest cohort (1396 VFs, 146 eyes with glaucoma). Read More

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October 2021

FTSH4 and OMA1 mitochondrial proteases reduce moderate heat stress-induced protein aggregation.

Plant Physiol 2021 Oct;187(2):769-786

Department of Cellular Molecular Biology, Faculty of Biotechnology, University of Wroclaw, Wroclaw, 50-383, Poland.

The threat of global warming makes uncovering mechanisms of plant tolerance to long-term moderate heat stress particularly important. We previously reported that Arabidopsis (Arabidopsis thaliana) plants lacking mitochondrial proteases FTSH4 or OMA1 suffer phenotypic changes under long-term stress of 30°C, while their growth at 22°C is not affected. Here we found that these morphological and developmental changes are associated with increased accumulation of insoluble mitochondrial protein aggregates that consist mainly of small heat-shock proteins (sHSPs). Read More

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October 2021

Quantification of mtDNA content in cultured cells by direct droplet digital PCR.

Mitochondrion 2021 Oct 1;61:102-113. Epub 2021 Oct 1.

Department of Physiology and Cell Biology, University of South Alabama, Mobile, AL 36688, USA. Electronic address:

Although alterations in cellular mitochondrial DNA (mtDNA) content have been linked to various pathological conditions, the mechanisms that govern mtDNA copy number (mtCN) control remain poorly understood. Moreover, techniques for mtDNA quantification do not allow for direct comparisons of absolute mtCNs between labs. Here we report the development of a direct droplet digital PCR technique for the determination of mtCNs in whole-cell lysates. Read More

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October 2021

Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, and .

J Proteome Res 2021 Oct 4. Epub 2021 Oct 4.

Department of Physical and Analytical Chemistry, University of Oviedo, 33006 Oviedo, Spain.

We report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here for two desert black cobra species within the genus , and . The experimental design includes the decomplexation of the venom samples by reverse-phase chromatography independently coupled to four mass spectrometry systems: the combined bottom-up and top-down molecular MS for protein identification and a parallel reverse-phase microbore high-performance liquid chromatograph (RP-μHPLC) on-line to inductively coupled plasma (ICP-MS/MS) elemental mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QToF MS). This allows to continuously record the absolute sulfur concentration throughout the chromatogram and assign it to the parent venom proteins separated in the RP-μHPLC-ESI-QToF parallel run via mass profiling. Read More

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October 2021

Topoisomerase Assays.

Curr Protoc 2021 Oct;1(10):e250

Departments of Immunology and Biochemistry, University of Washington, Seattle, Washington.

Topoisomerases are enzymes that play essential roles in DNA replication, transcription, chromosome segregation, and recombination. All cells have two major forms of DNA topoisomerases: type I enzymes, which make single-stranded cuts in DNA, and type II enzymes, which cut and decatenate double-stranded DNA. DNA topoisomerases are important targets of approved and experimental anti-cancer agents. Read More

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October 2021

A Portable Digital Loop-Mediated Isothermal Amplification Platform Based on Microgel Array and Hand-Held Reader.

ACS Sens 2021 Oct 4. Epub 2021 Oct 4.

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

Digital polymerase chain reaction (dPCR) has found widespread applications in molecular diagnosis of various diseases owing to its sensitive single-molecule detection capability. However, the existing dPCR platforms rely on the auxiliary procedure to disperse DNA samples, which needs complicated operation, expensive apparatus, and consumables. Besides, the complex and costly dPCR readers also impede the applications of dPCR for point-of-care testing (POCT). Read More

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October 2021

Isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative analysis of the salivary proteome during healthy pregnancy and pregnancy gingivitis.

J Clin Periodontol 2021 Oct 3. Epub 2021 Oct 3.

Singapore Oral Microbiomics Initiative, National Dental Research Institute Singapore, National Dental Center, Singapore.

Aim: The present study aimed to investigate the salivary proteome profiles of pregnant women with gingivitis (PG) or without gingivitis (HP) and non-pregnant healthy controls (HC) by employing iTRAQ-based proteomics.

Materials And Methods: Saliva samples were collected from 30 Chinese women comprising 10 subjects in each of the three groups (PG, HP, and HC). The samples were subjected to iTRAQ-based proteomics analysis, and ELISA was performed to validate the results. Read More

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October 2021

Development of a real-time RT-PCR method for the detection of virus (CTV) and its implication in studying virus distribution in planta.

3 Biotech 2021 Oct 11;11(10):431. Epub 2021 Sep 11.

Plant Virology Laboratory, ICAR-Central Citrus Research Institute, Nagpur, Maharashtra 440033 India.

Tristeza is an economically important disease of the citrus caused by virus (CTV) of genus  and family Closteroviridae. The disease has caused tremendous losses to citrus industry worldwide by killing millions of trees, reducing the productivity and total production. Enormous efforts have been made in many countries to prevent the viral spread and the losses caused by the disease. Read More

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October 2021

Comparison of different deep learning architectures for synthetic CT generation from MR images.

Phys Med 2021 Sep 29;90:99-107. Epub 2021 Sep 29.

Division of Nuclear Medicine and Molecular Imaging, Department of Medical Imaging, Geneva University Hospital, CH-1211 Geneva 4, Switzerland.

Purpose: Among the different available methods for synthetic CT generation from MR images for the task of MR-guided radiation planning, the deep learning algorithms have and do outperform their conventional counterparts. In this study, we investigated the performance of some most popular deep learning architectures including eCNN, U-Net, GAN, V-Net, and Res-Net for the task of sCT generation. As a baseline, an atlas-based method is implemented to which the results of the deep learning-based model are compared. Read More

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September 2021

Emerging methods for and novel insights gained by absolute quantification of mitochondrial DNA copy number and its clinical applications.

Pharmacol Ther 2021 Sep 27:107995. Epub 2021 Sep 27.

Biomedical Physiology & Kinesiology, Simon Fraser University, Burnaby, Canada; Centre for Cell Biology, Development, and Disease, Simon Fraser University, Burnaby, Canada. Electronic address:

The past thirty years have seen a surge in interest in pathophysiological roles of mitochondria, and the accurate quantification of mitochondrial DNA copy number (mCN) in cells and tissue samples is a fundamental aspect of assessing changes in mitochondrial health and biogenesis. Quantification of mCN between studies is surprisingly variable due to a combination of physiological variability and diverse protocols being used to measure this endpoint. The advent of novel methods to quantify nucleic acids like digital polymerase chain reaction (dPCR) and high throughput sequencing offer the ability to measure absolute values of mCN. Read More

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September 2021