7,779 results match your criteria ablation vivo

Interleukin-6 is an activator of pituitary stem cells upon local damage, a competence quenched in the aging gland.

Proc Natl Acad Sci U S A 2021 Jun;118(25)

Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute, Katholieke Universiteit Leuven, 3000 Leuven, Belgium;

Stem cells in the adult pituitary are quiescent yet show acute activation upon tissue injury. The molecular mechanisms underlying this reaction are completely unknown. We applied single-cell transcriptomics to start unraveling the acute pituitary stem cell activation process as occurring upon targeted endocrine cell-ablation damage. Read More

View Article and Full-Text PDF

Cascade targeting tumor mitochondria with CuS nanoparticles for enhanced photothermal therapy in the second near-infrared window.

Biomater Sci 2021 Jun 23. Epub 2021 Jun 23.

i-Lab and Division of Nanobiomedicine, CAS Key Laboratory of Nano-Bio Interface, CAS Center for Excellence in Nanoscience, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China. and School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei 230026, China.

Photothermal therapy, assisted by local heat generation using photothermal nanoparticles (NPs), is an emerging strategy to treat tumors noninvasively. To improve treatment outcomes and to alleviate potential side effects on normal tissue cells, utilizing the optically transparent second near-infrared (NIR-II) window and actively targeting tumors are critical. Considering that mitochondria are heat sensitive and play an important role in the up-regulation of metabolic activity in tumor cells, herein we report a cascade targeting scheme that enables active photothermal ablation of tumor mitochondria. Read More

View Article and Full-Text PDF

Rigidity Bridging Flexibility to Harmonize Three Excited-State Deactivation Pathways for NIR-II-Fluorescent-Imaging-Guided Phototherapy.

Adv Healthc Mater 2021 Jun 23:e2101003. Epub 2021 Jun 23.

Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, Chemical Biology Center, College of Chemistry, Central China Normal University, Wuhan, 430079, China.

Small organic phototherapeutic molecules of the second near-infrared (NIR-II) window (1000-1700 nm) serve as promising candidates for theranostics. However, developing such versatile agents for fluorescence-guided photodynamic/photothermal therapy remains a demanding task stirred by competitive energy dissipation pathways, including radiative decay, internal conversion, and intersystem crossing. To the best of current knowledge, the current paradigm for addressing the issue has deliberately approached the optimum balance among three deactivation processes through offsetting from each other, possibly leading to a comprehensively compromised theranostic efficacy. Read More

View Article and Full-Text PDF

ATE1 inhibits liver cancer progression through RGS5-mediated suppression of Wnt/β-catenin signaling.

Mol Cancer Res 2021 Jun 22. Epub 2021 Jun 22.

liver Cancer Laboratory, Department of Surgery, Xiangya Hospital Central South University

Arginyltransferase (ATE1) plays critical roles in many biological functions including cardiovascular development, angiogenesis, adipogenesis, muscle contraction, and metastasis of cancer. However, the role of ATE1 in hepatocellular carcinoma (HCC) remains unknown. In this study, we find that ATE1 plays an essential role in growth and malignancy of liver cancer. Read More

View Article and Full-Text PDF

Targeting HSPA8 inhibits proliferation via downregulating BCR-ABL and enhances chemosensitivity in imatinib-resistant chronic myeloid leukemia cells.

Exp Cell Res 2021 Jun 19:112708. Epub 2021 Jun 19.

Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, PR China.

The resistance to tyrosine kinase inhibitors is currently a major problem for chronic myeloid leukemia (CML) treatment and HSPA8 is highly expressed and a hallmark of poor prognosis in several human cancers. However, its role in imatinib-resistant CML (IR-CML) cells remains undetermined. Here, we determined HSPA8 was overexpressed in IR-CML cells and associated with imatinib resistance. Read More

View Article and Full-Text PDF

Near-Infrared Light-Driven Multifunctional Tubular Micromotors for Treatment of Atherosclerosis.

ACS Appl Mater Interfaces 2021 Jun 22. Epub 2021 Jun 22.

Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China.

One of the difficulties in atherosclerosis treatment is that the ablation of inflammatory macrophages, repair of vascular endothelial injury, and anti-tissue proliferation should be considered. However, there are few studies that can solve the abovementioned problems simultaneously. Herein, we present a kind of near-infrared (NIR) light-driven multifunctional mesoporous/macroporous tubular micromotor which can rapidly target the damaged blood vessels and release different drugs. Read More

View Article and Full-Text PDF

Rictor is a novel regulator of TRAF6/TRAF3 in osteoclasts.

J Bone Miner Res 2021 Jun 22. Epub 2021 Jun 22.

Department of Cell Biology, School of Basic Medical Science, Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Southern Medical University, Guangzhou, 510515, China.

Tumor necrosis factor receptor-associated factors (TRAFs) are crucial for receptor activator of nuclear factor-κB (RANK) activation in osteoclasts. However, the upstream mechanisms of TRAF members in the osteoclastic lineage remain largely unknown. Here, we demonstrated that Rictor, a key component of mechanistic target of rapamycin complex 2 (mTORC2), was crucial for TRAF6/TRAF3 expression in osteoclasts. Read More

View Article and Full-Text PDF

A Novel Controlled PTEN-Knockout Mouse Model for Prostate Cancer Study.

Front Mol Biosci 2021 3;8:696537. Epub 2021 Jun 3.

Department of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA, United States.

Prostate cancer (PCa) is associated with advanced age, but how age contributes to prostate carcinogenesis remains unknown. The prostate-specific Pten conditional knockout mouse model closely imitates human PCa initiation and progression. To better understand how age impacts PCa in an experimental model, we have generated a spatially and temporally controlled Pten-null PCa murine model at different ages (aged vs. Read More

View Article and Full-Text PDF

Deep-Tissue Photothermal Therapy Using Laser Illumination at NIR-IIa Window.

Nanomicro Lett 2020 Jan 24;12(1):38. Epub 2020 Jan 24.

Molecular Imaging Program at Stanford, Stanford University, Palo Alto, CA, 94301, USA.

Photothermal therapy (PTT) using near-infrared (NIR) light for tumor treatment has triggered extensive attentions because of its advantages of noninvasion and convenience. The current research on PTT usually uses lasers in the first NIR window (NIR-I; 700-900 nm) as irradiation source. However, the second NIR window (NIR-II; 1000-1700 nm) especially NIR-IIa window (1300-1400 nm) is considered much more promising in diagnosis and treatment as its superiority in penetration depth and maximum permissible exposure over NIR-I window. Read More

View Article and Full-Text PDF
January 2020

Functional development and regeneration of hair cells in the zebrafish lateral line.

J Physiol 2021 Jun 18. Epub 2021 Jun 18.

Department of Biomedical Science, University of Sheffield, Sheffield, S10 2TN, UK.

Key Points: We investigated hair-cell regeneration in the zebrafish lateral line following the application of the ototoxic compound copper. In early-larval zebrafish (<10-days post-fertilization), regenerated hair cells drive action potentials (APs) in the afferent neurons 24-hours post-copper treatment (24 hpt). Full regeneration of the early-larval neuromasts, the organs containing the hair cells, requires ∼48 hours due to the progressive addition of hair cells and synaptic refinement. Read More

View Article and Full-Text PDF

Evaluation of Microwave Ablation Efficacy by Strain Elastography and Shear Wave Elastography in ex Vivo Porcine Liver.

Ultrasound Med Biol 2021 Jun 14. Epub 2021 Jun 14.

Department of Thoracic Surgery, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, Guangdong Province, China. Electronic address:

The aim of this study was to evaluate the efficacy of microwave ablation by ultrasound (US), strain elastography (SE) and shear-wave elastography (SWE). An ex vivo model of porcine liver was adopted. According to ablation power and duration, 30 samples were divided into three groups: group 1 (45 W, 30 s), group 2 (45 W, 15 s) and group 3 (30 W, 30 s). Read More

View Article and Full-Text PDF

Dual Targeting Smart Drug Delivery System for Multimodal Synergistic Combination Cancer Therapy with Reduced Cardiotoxicity.

Acta Biomater 2021 Jun 14. Epub 2021 Jun 14.

Institute of radiation and Polymer Technology, Bangladesh Atomic Energy Commission, Dhaka 1000, Bangladesh. Electronic address:

This study first reports the development of a smart drug delivery system (DDS) for multimodal synergistic cancer therapy combining chemo-photothermal-starvation approaches. A magnetic photothermal agent was synthesized by preparing iron oxide (IO) nanoparticles (NPs) with covalently attached indocyanine green (ICG) and glucose oxidase (GOx) ([email protected]). Synthesized [email protected] NPs were co-encapsulated with doxorubicin (Dox) and EGCG ((-)-epigallocatechin-3-gallate) inside PLGA (poly(lactic-co-glycolic acid)) NPs using multiple emulsion solvent evaporation method. Read More

View Article and Full-Text PDF

Comparison of Tissue Temperature Profile and Lesion Geometry for Radiofrequency Ablation with High Power-Short Duration and Moderate Power-Moderate Duration: Effects of Thermal Latency and Contact Force on Lesion Formation.

Circ Arrhythm Electrophysiol 2021 Jun 17. Epub 2021 Jun 17.

Heart Rhythm Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK.

- With short radiofrequency (RF) applications, tissue temperature continues to rise after RF-termination ("thermal latency"), which may result in lesion growth after RF-termination. The purpose was to compare tissue temperature profile (thermal latency), lesion size and the incidence of steam pop and thrombus between RF-ablation with very-high-power-very-short-RF(90W/4s), high-power-short-RF(50W/10s) and moderate-power-moderate-RF(30W/30s) in a canine thigh muscle preparation and beating heart. - In the thigh muscle preparation (5dogs), a 3. Read More

View Article and Full-Text PDF

MYC-mediated early glycolysis negatively regulates proinflammatory responses by controlling IRF4 in inflammatory macrophages.

Cell Rep 2021 Jun;35(11):109264

Arthritis and Tissue Degeneration Program, David Z. Rosensweig Genomics Research Center, Hospital for Special Surgery, New York, NY, USA; Department of Medicine, Weill Cornell Medical College, New York, NY, USA; BCMB Allied Program, Weill Cornell Graduate School of Medical Sciences, New York, NY, USA. Electronic address:

MYC activates different metabolic programs in a cell-type- and cell-status-dependent manner. However, the role of MYC in inflammatory macrophages has not yet been determined. Metabolic and molecular analyses reveal that MYC, but not hypoxia inducible factor 1 (HIF1), is involved in enhancing early glycolytic flux during inflammatory macrophage polarization. Read More

View Article and Full-Text PDF

Apoferritin-Engineered Nanoprobe for Tumor-Targeted Triple-NIR Imaging and Phototherapy.

Anal Chem 2021 Jun 14. Epub 2021 Jun 14.

State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China.

Considering that pathological hallmarks are directly related to structural and chemical information of tumor, noninvasive, real-time, spatially resolved quantitative chemical imaging is significant for treatment decisions. The discovery of the transparency window of biological tissues and the advancement of near-infrared technology provide exciting prospects for imaging. Herein, an engineering apoferritin-conjugated cypate nanoprobe is fabricated for near-infrared photoacoustic imaging and fluorescence imaging in the first and second window. Read More

View Article and Full-Text PDF

NLRP3 Inflammasome Assembly in Neutrophils Is Supported by PAD4 and Promotes NETosis Under Sterile Conditions.

Front Immunol 2021 28;12:683803. Epub 2021 May 28.

Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, United States.

Neutrophil extracellular trap formation (NETosis) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome assembly are associated with a similar spectrum of human disorders. While NETosis is known to be regulated by peptidylarginine deiminase 4 (PAD4), the role of the NLRP3 inflammasome in NETosis was not addressed. Here, we establish that under sterile conditions the cannonical NLRP3 inflammasome participates in NETosis. Read More

View Article and Full-Text PDF

Assessing Early Cardiac Outflow Tract Adaptive Responses Through Combined Experimental-Computational Manipulations.

Ann Biomed Eng 2021 Jun 11. Epub 2021 Jun 11.

Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, 304 Weill Hall, Ithaca, NY, 14853-7202, USA.

Mechanical forces are essential for proper growth and remodeling of the primitive pharyngeal arch arteries (PAAs) into the great vessels of the heart. Despite general acknowledgement of a hemodynamic-malformation link, the direct correlation between hemodynamics and PAA morphogenesis remains poorly understood. The elusiveness is largely due to difficulty in performing isolated hemodynamic perturbations and quantifying changes in-vivo. Read More

View Article and Full-Text PDF

Caldesmon ablation in mice causes umbilical herniation and alters contractility of fetal urinary bladder smooth muscle.

J Gen Physiol 2021 Jul 11;153(7). Epub 2021 Jun 11.

Institute of Vegetative Physiology, Center of Physiology, Faculty of Medicine, University of Cologne, Cologne, Germany.

The actin-, myosin-, and calmodulin-binding protein caldesmon (CaD) is expressed in two splice isoforms: h-CaD, which is an integral part of the actomyosin domain of smooth muscle cells, and l-CaD, which is widely expressed and is involved in many cellular functions. Despite extensive research for many years, CaD's in vivo function has remained elusive. To explore the role of CaD in smooth muscle contraction in vivo, we generated a mutant allele that ablates both isoforms. Read More

View Article and Full-Text PDF

Enzymatically synthesised MnO nanoparticles for efficient near-infrared photothermal therapy and dual-responsive magnetic resonance imaging.

Nanoscale 2021 Jun 11. Epub 2021 Jun 11.

School of Chemistry and Chemical Engineering, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, Huazhong University of Science and Technology, Wuhan 430074, China.

Manganese dioxide (MnO2) nanoparticles (NPs) are highly attractive for biomedical applications due to their biocompatibility, stimuli-responsive magnetic resonance imaging (MRI) properties and capability to modulate the hypoxic tumour microenvironment (TME). However, conventional MnO2 NPs do not possess photothermal therapy (PTT) functions except for hybrids with other photothermal materials. Herein, we first reveal the extraordinary photothermal conversion efficiency (44%) of enzymatically synthesised MnO2 NPs (Bio-MnO2 NPs), which are distinct from chemically synthesised MnO2 NPs. Read More

View Article and Full-Text PDF

Bipolar ablation's unique paradigm: Duration and power as respectively distinct primary determinants of transmurality and steam pop formation.

Heart Rhythm O2 2020 Oct 24;1(4):290-296. Epub 2020 Jun 24.

Department of Electrophysiology Clinical Research and Innovations, Texas Heart Institute, Houston, Texas.

Background: Bipolar radiofrequency (RF) ablation strategies are increasingly used, mainly to target deep myocardial reentrant circuits responsible for ventricular tachycardia that cannot be extinguished with traditional unipolar RF ablation. Because this strategy is novel, factors that affect lesion geometry and steam pop formation require further investigation.

Objective: To assess the effect of contact force, power, and time on the resulting lesion geometry and the risk of steam pop formation during bipolar RF ablation of thick myocardial tissue. Read More

View Article and Full-Text PDF
October 2020

The role of Mfn2 in the structure and function of ER-mitochondrial tethering in vivo.

J Cell Sci 2021 Jun 10. Epub 2021 Jun 10.

Department of Pathology, Case Western Reserve University, Cleveland, OH, USA.

The mitochondria-ER contacts (MERCs) plays an essential role in multiple cell physiological process. While Mfn2 was the first protein implicated in the formation of MERCs, it is debated whether it acts as a tether or antagonizer, largely based on in vitro studies. To understand the role of Mfn2 in MERCs in vivo, we characterized ultrastructural and biochemical changes of MERCs in pyramidal neurons of hippocampus in Mfn2 conditional knockout (KO) mice and in Mfn2 overexpression (OE) mice and found Mfn2 ablation caused reduced close contacts while Mfn2 OE caused increased close contacts between ER and mitochondria in vivo. Read More

View Article and Full-Text PDF

Targeting the miR-6734-3p/ZEB2 axis hampers development of non-small cell lung cancer (NSCLC) and increases susceptibility of cancer cells to cisplatin treatment.

Bioengineered 2021 Dec;12(1):2499-2510

Department of Respiratory and Critical Care Medicine, People's Hospital of Quzhou, Quzhou City, Zhejiang Province, China.

The unclear pathogenesis mechanisms and resistance of cancer cells to chemical drugs serious limits the development of effective treatment strategies for non-small cell lung cancer (NSCLC). In this study, we managed to investigate this issue, and identify potential cancer associated biomarkers for NSCLC diagnosis, prognosis and treatment. This study found that miR-6734-3p was downregulated in both NSCLC clinical specimens (tissues and serum) and cells, compared to the normal tissues and cells. Read More

View Article and Full-Text PDF
December 2021

miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3.

Cell Rep 2021 Jun;35(10):109225

CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, China; Department of Plastic & Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China. Electronic address:

Maintaining a suitable level of sensitivity to environmental cues is crucial for proper function of adult stem cells. Here, we explore how the intrinsic sensitivity of skin hair follicle (HF) progenitors to growth stimuli is dynamically regulated. We discover miR-24 is an miRNA whose expression in HF progenitors inversely correlates with their growth potency in vivo. Read More

View Article and Full-Text PDF

Transglutaminase 2 promotes tumorigenicity of colon cancer cells by inactivation of the tumor suppressor p53.

Oncogene 2021 Jun 8. Epub 2021 Jun 8.

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany.

Despite a high clinical need for the treatment of colorectal carcinoma (CRC) as the second leading cause of cancer-related deaths, targeted therapies are still limited. The multifunctional enzyme Transglutaminase 2 (TGM2), which harbors transamidation and GTPase activity, has been implicated in the development and progression of different types of human cancers. However, the mechanism and role of TGM2 in colorectal cancer are poorly understood. Read More

View Article and Full-Text PDF

Silencing of cystatin SN (CST1) abrogates cancer progression and stem cell properties in papillary thyroid carcinoma (PTC).

FEBS Open Bio 2021 Jun 8. Epub 2021 Jun 8.

Department of Ultrasound, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Liyushan South Road No.137, Urumqi, 830054, People's Republic of China.

Papillary thyroid carcinoma (PTC) accounts for approximately 80% of total thyroid cancer worldwide. Although the prognosis for early-stage PTC is favorable, the 5-year survival rate of patients with late-stage PTC is still very poor. Cystatin SN (cystatin 1, CST1) facilitates the progression of multiple cancers, but its role in regulating papillary thyroid carcinoma (PTC) pathogenesis is still largely unknown. Read More

View Article and Full-Text PDF

A Synergistic DNA-polydopamine-MnO Nanocomplex for Near-Infrared-Light-Powered DNAzyme-Mediated Gene Therapy.

Nano Lett 2021 Jun 8;21(12):5377-5385. Epub 2021 Jun 8.

Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, People's Republic of China.

DNAzyme is emerging for gene therapy. The administration of the catalytic activity of DNAzyme has proven important but challenging for clinical applications. Herein, we report a synergistic DNA-polydopamine-MnO nanocomplex, which enables near-infrared (NIR)-light-powered catalytic activity of DNAzyme . Read More

View Article and Full-Text PDF

A NIR-I light-responsive superoxide radical generator with cancer cell membrane targeting ability for enhanced imaging-guided photodynamic therapy.

Chem Sci 2020 Sep 5;11(37):10279-10286. Epub 2020 Sep 5.

Hefei National Laboratory for Physical Sciences at Microscale, iChem, University of Science and Technology of China Hefei P. R. China.

Photodynamic therapy (PDT), as an emerging treatment modality, which takes advantage of reactive oxygen species (ROS) generated upon light illumination to ablate tumours, has suffered from a limited treatment depth, strong oxygen dependence and short ROS lifespan. Herein, we developed a highly efficient NIR-I light (808 nm laser) initiated theranostic system based on a fluorescent photosensitizer (EBD-1) with cancer cell membrane targeting ability, which can realize large penetration depth in tissue, generate superoxide radicals (O˙) to relieve the oxygen-dependence, confine the ROS oxidation at the cell membrane, and self-report the cell viability during the PDT process. experiments demonstrated that EBD-1 under 808 nm light successfully accomplished remarkable cancer ablation. Read More

View Article and Full-Text PDF
September 2020

γ-Glutamyl transpeptidase-activatable near-infrared nanoassembly for tumor fluorescence imaging-guided photothermal therapy.

Theranostics 2021 13;11(14):7045-7056. Epub 2021 May 13.

School of Engineering, China Pharmaceutical University, Nanjing 211198, P. R. China.

Precise treatment of tumors is attracting increasing attention. Molecular probes simultaneously demonstrating the diagnostic signal and pharmacological effect in response to tumor microenvironment are highly desired. γ-glutamyl transpeptidase (GGT) is a biomarker with significantly up-regulated expression in the tumor area. Read More

View Article and Full-Text PDF

Cathepsin B-responsive multifunctional peptide conjugated gold nanorods for mitochondrial targeting and precise photothermal cancer therapy.

J Colloid Interface Sci 2021 May 26;601:714-726. Epub 2021 May 26.

Department of Polymer Materials, Zhejiang Sci-Tech University, Hangzhou 310018, China. Electronic address:

Nanomaterials have shown great potential in cancer therapy, but the phenomenon of poor tumor recognition without cellular organelle accumulation usually leads to reduced therapeutic effects and enhanced side effects. Herein, we resolved this issue by employing a multifunctional peptide coating mainly composed of, from the inside out, a mitochondrial targeting segment, a cathepsin B-responsive segment and a zwitterionic antifouling segment. Then gold nanorods were modified with a peptide via ligand exchange, displaying excellent photothermal property and superior stability both before and after enzyme treatment. Read More

View Article and Full-Text PDF

RNA-guided gene editing of the murine gammaherpesvirus 68 genome reduces infectious virus production.

PLoS One 2021 4;16(6):e0252313. Epub 2021 Jun 4.

Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York, United States of America.

Epstein-Barr virus (EBV) and Kaposi sarcoma herpesvirus (KSHV) are cancer-causing viruses that establish lifelong infections in humans. Gene editing using the Cas9-guideRNA (gRNA) CRISPR system has been applied to decrease the latent load of EBV in human Burkitt lymphoma cells. Validating the efficacy of Cas9-gRNA system in eradicating infection in vivo without off-target effects to the host genome will require animal model systems. Read More

View Article and Full-Text PDF