28,320 results match your criteria a549 human


microRNA-204 shuttled by mesenchymal stem cell-derived exosomes inhibits the migration and invasion of non-small-cell lung cancer cells via the KLF7/AKT/HIF-1α axis.

Neoplasma 2021 Apr 13. Epub 2021 Apr 13.

Department of Respiratory Medicine, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China.

Non-small-cell lung cancer (NSCLC) remains the leading cause of cancer-related death worldwide. Accumulating researches have highlighted the ability of exosome-encapsulated microRNAs (miRNAs or miRs) as potential circulating biomarkers for lung cancer. The current study aimed to evaluate the significance of mesenchymal stem cells (MSCs)-derived exosomal miR-204 in the invasion, migration, and epithelial-mesenchymal transition (EMT) of NSCLC cells. Read More

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Effect of long noncoding RNAs on epithelial-mesenchymal transition in A549 cells and fibrotic human lungs.

J Cell Biochem 2021 Apr 13. Epub 2021 Apr 13.

Department of Thoracic Surgery, Lung Transplantation Center, Kartal Kosuyolu High Specialty Educational and Research Hospital, Istanbul, Turkey.

Long noncoding RNAs (LncRNAs) regulate epithelial-mesenchymal transition (EMT). EMT involves myofibroblast differentiation and pulmonary fibrosis (PF). We aimed to determine the expression profiles of HOTAIR, CARLo-5, and CD99P1 LncRNAs in EMT-mediated myofibroblast differentiation in A549 cells and fibrotic human lungs and to explain their roles. Read More

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Inhibitory effects of terrein on lung cancer cell metastasis and angiogenesis.

Oncol Rep 2021 Jun 13;45(6). Epub 2021 Apr 13.

Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand.

Cancer metastasis is the leading cause of mortality in cancer patients. Over 70% of lung cancer patients are diagnosed at advanced or metastatic stages, and this results in an increased incidence of mortality. Terrein is a secondary bioactive fungal metabolite isolated from . Read More

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Metformin impairs cisplatin resistance effects in A549 lung cancer cells through mTOR signaling and other metabolic pathways.

Int J Oncol 2021 Jun 13;58(6). Epub 2021 Apr 13.

Multidisciplinary Laboratory of Food and Health, School of Applied Sciences, State University of Campinas, Limeira, SP 13484‑350, Brazil.

Lung cancer is the leading cause of cancer‑associated death worldwide and exhibits intrinsic and acquired therapeutic resistance to cisplatin (CIS). The present study investigated the role of mTOR signaling and other signaling pathways after metformin (MET) treatment in control and cisplatin‑resistant A549 cells, mapping pathways and possible targets involved in CIS sensitivity. MTT, flow cytometry, clonogenic assay, western blotting, proteomic analysis using the Stable Isotope Labeling by Amino acids in Cell culture (SILAC) approach and reverse transcription‑quantitative PCR were performed. Read More

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[Retracted] Daidzein exerts anticancer activity towards SKOV3 human ovarian cancer cells by inducing apoptosis and cell cycle arrest, and inhibiting the Raf/MEK/ERK cascade.

Int J Mol Med 2021 Jun 13;47(6). Epub 2021 Apr 13.

Department of Gynecology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China.

Following the publication of the above paper, a concerned reader drew to the Editor's attention that several figures contained data that bore striking similarities to data published in other papers; notably, the western blot data shown in Fig. 6 appeared to have been presented in other studies, notably in Fig. 7B of another paper published around the same time and written by different authors based at different research institutions [Li P, Zhang Z, Zhang F, Zhou H and Sun W: Effects of 3‑tetrazolyl methyl‑3‑hydroxy‑oxindole hybrid (THOH) on cell proliferation, apoptosis, and G2/M cell cycle arrest occurs by targeting platelet‑derived growth factor D (PDGF‑D) and the MEK/ERK signaling pathway in human lung cell lines SK‑LU‑1, A549, and A‑427. Read More

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BIODISTRIBUTION OF FLOURESCENCE-LABELLED EGF PROTEIN FROM SLOW RELEASE NANOZOLID DEPOTS IN MOUSE.

Int J Pharm 2021 Apr 9:120588. Epub 2021 Apr 9.

Experimental Cancer Medicine, Clinical Research Center, Karolinska Institutet, Stockholm Sweden; Clinical Research Center (KFC) and Center for Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital Huddinge, Stockholm, Sweden. Electronic address:

Aim: The study was designed to evaluate the ability of the calcium sulfate based NanoZolid® drug delivery technology to locally release the epidermal growth factor (EGF) protein while maintaining its biological activity.

Methods: NanoZolid-formulated EGF protein labelled with a near infrared dye (EGF-NIR) depots or EGF-NIR dissolved in PBS were injected subcutaneously into mice bearing EGF receptor (EGFR) positive human A549 lung cancer tumors inoculated subcutaneously. The release and biodistribution of the EGF-NIR were investigated in vivo longitudinally up to 96 hours post administration, utilizing whole body fluorescence imaging. Read More

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Histone Deacetylase Inhibitory and Cytotoxic Activities of the Constituents from the Roots of .

Iran J Pharm Res 2020 ;19(4):51-58

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Four prenylated flavonoids, including isosophoranone, sophoraflavanone G, alopecurone J, alopecurone P, and a resveratrol derivative HPD (2-(4-hydroxyphenyl)-2,3-dihydrobenzo[b] furan-3,4,6-triol), were isolated from the roots of . The cytotoxic activity of obtained compounds was evaluated against A2780, A549, HeLa, and HCT116 human cancer cell lines. We also evaluated their histone deacetylase (HDAC) inhibitory activities. Read More

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January 2020

Inhibitory effects of lactoferrin on pulmonary inflammatory processes induced by lipopolysaccharide through modulating the TLR4-related pathway.

J Dairy Sci 2021 Apr 7. Epub 2021 Apr 7.

Key Laboratory of Quality and Safety Control for Milk and Dairy Products, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China. Electronic address:

This study tested the ability of lactoferrin to modulate pulmonary inflammation. To construct in vitro and in vivo inflammatory lung models, cells from the human lung adenocarcinoma cell line (A549) were exposed to lipopolysaccharide (LPS, 1 µg/mL), and mice (CD-1) were intratracheally administrated LPS [10 mg/kg of body weight (BW), tracheal lumen injection], respectively. The A549 cells were preincubated with lactoferrin (10 mg/mL), and the mice were intraperitoneally injected with lactoferrin (100 mg/kg of BW), followed by LPS treatment. Read More

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A novel method for sensor-based quantification of single/multicellular force dynamics and stiffening in 3D matrices.

Sci Adv 2021 Apr 9;7(15). Epub 2021 Apr 9.

Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Cells in vivo generate mechanical traction on the surrounding 3D extracellular matrix (ECM) and neighboring cells. Such traction and biochemical cues may remodel the matrix, e.g. Read More

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Novel chiral naphthalimide-cycloalkanediamine conjugates: Design, synthesis and antitumor activity.

Bioorg Chem 2021 Mar 24;112:104859. Epub 2021 Mar 24.

EntreChem SL, Vivero Ciencias de la Salud, Santo Domingo de Guzmán, 33011 Oviedo, Spain. Electronic address:

A novel series of enantiopure naphthalimide-cycloalkanediamine conjugates were designed, synthetized and evaluated for in vitro cytotoxicity against human colon adenocarcinoma (LoVo), human lung adenocarcinoma (A549), human cervical carcinoma (Hela) and human promyelocytic leukemia cell lines (HL-60). The cytotoxicity of the compounds was highly dependent on size and relative stereochemistry of the cycloalkyl ring as well as length of the spacer. By contrast, any kind of enantioselection was observed for each pair of enantiomers. Read More

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Affinity of Mycobacterium tuberculosis strains for M059K microglial cells after migration through A549 alveolar epithelium.

Eur J Clin Microbiol Infect Dis 2021 Apr 9. Epub 2021 Apr 9.

Department of Medical Microbiology, Nelson R Mandela School of Medicine, School of Laboratory Medicine and Medical Science, University of KwaZulu-Natal, Durban, South Africa.

Tuberculosis (TB) remains a major threat worldwide while central nervous system TB (CNS-TB) is one of the most severe forms of extrapulmonary TB. CNS-TB develops as a secondary infection during the hematogenous spread of Mycobacterium tuberculosis (M. tuberculosis) from the lungs to the CNS. Read More

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Circulating miR-320a Acts as a Tumor Suppressor and Prognostic Factor in Non-small Cell Lung Cancer.

Front Oncol 2021 23;11:645475. Epub 2021 Mar 23.

Department of Zoology, Central University of Punjab, Bathinda, India.

Dysregulated expression profiles of microRNAs (miRNAs) have been observed in several types of cancer, including non-small cell lung cancer (NSCLC); however, the diagnostic and prognostic potential of circulating miRNAs in NSCLC remains largely undefined. Here we found that circulating miR-320a was significantly down-regulated (~5.87-fold; < 0. Read More

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POU2F2 promotes the proliferation and motility of lung cancer cells by activating AGO1.

BMC Pulm Med 2021 Apr 8;21(1):117. Epub 2021 Apr 8.

Department of Thoracic Surgery, The First Affiliated Hospital of Fujian Medical University, No.20 Chazhong Road, Fuzhou City, 350005, Fujian Province, China.

Background: To detect and investigate the expression of POU domain class 2 transcription factor 2 (POU2F2) in human lung cancer tissues, its role in lung cancer progression, and the potential mechanisms.

Methods: Immunohistochemical (IHC) assays were conducted to assess the expression of POU2F2 in human lung cancer tissues. Immunoblot assays were performed to assess the expression levels of POU2F2 in human lung cancer tissues and cell lines. Read More

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Targeting cyclin-dependent kinase 6 by vanillin inhibits proliferation of breast and lung cancer cells: Combined computational and biochemical studies.

J Cell Biochem 2021 Apr 8. Epub 2021 Apr 8.

Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, India.

Cyclin-dependent kinase 6 (CDK6) is a member of serine/threonine kinase family, and its overexpression is associated with cancer development. Thus, it is considered as a potential drug target for anticancer therapies. This study showed the CDK6 inhibitory potential of vanillin using combined experimental and computational methods. Read More

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Profiling the interaction of a novel toxic pyruvate dehydrogenase kinase inhibitor with human serum albumin.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Mar 23;256:119733. Epub 2021 Mar 23.

School of Food & Biological Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address:

To discover novel pyruvate dehydrogenase kinase (PDK) inhibitors, a new compound 2,2-dichloro-1-(4-((4-isopropylphenyl)amino)-3-nitrophenyl)ethan-1-one, namely XB-1 was identified, which inhibited PDK activity with a half maximal inhibitory concentration (IC) value of 337.0 nM, and reduced A549 cell proliferation with a half maximal effective concentration (EC) value of 330.0 nM. Read More

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Bioactive half-sandwich Rh and Ir bipyridyl complexes containing artemisinin.

J Inorg Biochem 2021 Mar 3;219:111408. Epub 2021 Mar 3.

Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK. Electronic address:

Reaction of dihydroartemisinin (DHA) with 4-methyl-4'-carboxy-2,2'-bipyridine yielded the new ester derivative L1. Six novel organometallic half-sandwich chlorido Rh(III) and Ir(III) complexes (1-6) containing pentamethylcyclopentadienyl, (Cp*), tetramethylphenylcyclopentadienyl (Cp), or tetramethylbiphenylcyclopentadienyl (Cp), and N,N-chelated bipyridyl group of L1, have been synthesized and characterized. The complexes were screened for inhibitory activity against the Plasmodium falciparum 3D7 (sensitive), Dd2 (multi-drug resistant) and NF54 late stage gametocytes (LSGNF54), the parasite strain Trichomonas vaginalis G3, as well as A2780 (human ovarian carcinoma), A549 (human alveolar adenocarcinoma), HCT116 (human colorectal carcinoma), MCF7 (human breast cancer) and PC3 (human prostate cancer) cancer cell lines. Read More

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Targeting stanniocalcin-1-expressing tumor cells elicits efficient antitumor effects in a mouse model of human lung cancer.

Cancer Med 2021 Apr 7. Epub 2021 Apr 7.

Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.

Lung cancer is the most common cause of cancer-related death in developed countries; therefore, the generation of effective targeted therapeutic regimens is essential. Recently, gene therapy approaches toward malignant cells have emerged as attractive molecular therapeutics. Previous studies have indicated that stanniocalcin-1 (STC-1), a hormone involved in calcium and phosphate homeostasis, positively regulates proliferation, apoptosis resistance, and glucose metabolism in lung cancer cell lines. Read More

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Geraniol exerts its antiproliferative action by modulating molecular targets in lung and skin carcinoma cells.

Phytother Res 2021 Apr 7. Epub 2021 Apr 7.

Bioprospection and Product Development Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.

Geraniol, an acyclic monoterpene present in several plant species' essential oils, is utilized as a food additive. It possesses potent antiproliferative and antitumor effects ascribed to its antiinflammatory, and antioxidant properties. The study aimed to understand geraniol's mechanism in human lung and skin cancer cells by employing molecular and cell target-based assays. Read More

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