17,784 results match your criteria Yersinia Enterocolitica


Fluorescence polarization assay: Diagnostic evaluation for porcine brucellosis.

J Microbiol Methods 2018 Dec 3. Epub 2018 Dec 3.

Indian Council Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru 560064, India. Electronic address:

Brucellosis in pigs, caused by the bacterium Brucella suis, is an important zoonotic infection. In the present study, fluorescence polarization assay (FPA) was standardized and compared with indirect enzyme linked immunosorbent assay (iELISA) and competitive ELISA (cELISA) for diagnosis of porcine brucellosis. Test performances were evaluated using representative panel (n = 100), samples from swine brucellosis outbreak (n = 300), samples from brucellosis suspected animals (n = 291) and sera samples from apparently healthy animals (n = 1121). Read More

View Article
December 2018
2 Reads

The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM.

Virulence 2018 Dec 5. Epub 2018 Dec 5.

a Department of Molecular Biology , Umeå University , Umeå , Sweden.

The Gram-negative enteropathogen Yersinia pseudotuberculosis possesses a number of regulatory systems that detect cell envelope damage caused by noxious extracytoplasmic stresses. The CpxA sensor kinase and CpxR response regulator two-component regulatory system is one such pathway. Active Cpx signalling upregulates various factors designed to repair and restore cell envelope integrity. Read More

View Article
December 2018
1 Read

A versatile remote control system for functional expression of bacterial virulence genes based on the tetA promoter.

Int J Med Microbiol 2018 Nov 16. Epub 2018 Nov 16.

Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, Barbarastr. 11, 49076, Osnabrück, Germany. Electronic address:

The expression of bacterial virulence factors is controlled in response to host or environmental factors and most virulence genes are not expressed under laboratory conditions. Investigations of molecular structures and cellular functions of bacterial virulence factors demand systems for experimentally controlled expression. We describe a simple and robust system that is based on the tetA promoter and the cognate repressor TetR. Read More

View Article
November 2018

Performance of Stool Testing Recommendations for Acute Gastroenteritis when Used to Identify Children with Nine Potential Bacterial Enteropathogens.

Clin Infect Dis 2018 Dec 4. Epub 2018 Dec 4.

Sections of Pediatric Emergency Medicine and Gastroenterology, Department of Pediatrics, Alberta Children's Hospital Research Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.

Background: The ability to identify bacterial pathogens that necessitate specific clinical management or public health action in children with acute gastroenteritis is crucial to patient care and public health. However, existing stool testing guidelines offer inconsistent recommendations, and their performance characteristics are unknown. We evaluated six leading gastroenteritis guidelines (e. Read More

View Article
December 2018
3 Reads

Expression and biochemical characterization of a Yersinia intermedia phytase expressed in Escherichia coli.

Recent Pat Food Nutr Agric 2018 Dec 4. Epub 2018 Dec 4.

Laboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296. Brazil.

Background: Phytases are enzymes capable of degrade phytic acid and used in animal feed supplementation in order to impro digestibility throught the release of mineral such phosphorus.

Objective: The main goal of this study was to express and characterize of a Yersinia intermedia phytase expressed in Escherichia coli cells.

Methods: The Y. Read More

View Article
December 2018

La réémergence de la peste.

Rev Prat 2016 Apr;66(4):413-418

Unité de recherche Yersinia, Centre national de référence et Centre collaborateur OMS pour les Yersinia, Institut Pasteur, Paris, France.

Reemergence of plague. Plague was one of the most devastating diseases of human history until vaccination and antibiotic therapy considerably reduced the number of cases. Nowadays plague tends to be considered a disease of ancient times. Read More

View Article

The effects of common yarrow (Achillea millefolium Linnaeus), cinnamon (Cinnamomum zeylanicum Blume) and rosemary (Rosemarinus officinalis Linnaeus) hydrosols on the some immunological and hematological parameters of common carp (Cyprinus carpio L., 1758).

Cell Mol Biol (Noisy-le-grand) 2018 Nov 30;64(14):19-24. Epub 2018 Nov 30.

Fisheries Faculty, Munzur University, TR62000, Tunceli, Turkey.

In this study, the effects of some plant hydrosols (distilled plant waters) based upon some hematological parameters and Nitroblue Tetrazolium (NBT) activities in the common carp (Cyprinus carpio Linnaeus, 1758) infected with Yersinia ruckeri were investigated. In the trial, it was utilized totally 200 common carps with 54.3±6. Read More

View Article
November 2018

Escherichia coli expressed flagellin C (FliC) of Salmonella Typhi improved the protective efficacy of YopE against plague infection.

Vaccine 2019 Jan 26;37(1):19-24. Epub 2018 Nov 26.

Microbiology Division, Defence Research & Development Establishment, Jhansi Road, Gwalior 474002, India.

In the current antibiotic resistance scenario, vaccines may provide best defense against lethal bacterial diseases. So far, there is no idealvaccine available against plague. Despite providing complete protection in small animal models, F1/LcrV based vaccine failed to provide ideal protection in non human primates. Read More

View Article
January 2019

Microbial contamination of moose (Alces alces) and white-tailed deer (Odocoileus virginianus) carcasses harvested by hunters.

Food Microbiol 2019 Apr 14;78:82-88. Epub 2018 Sep 14.

Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Finland.

Hunting is currently a very popular activity, and interest in game meat is increasing. However, only limited research is available on the bacterial quality and safety of moose (Alces alces) and white-tailed deer (Odocoileus virginianus) harvested by hunters. Poor hunting hygiene can spread bacteria onto the carcasses, and inadequate chilling of the carcasses may increase the bacterial load on the carcass surface. Read More

View Article
April 2019
1 Read

Identification of specific sequence motif of YopN of Yersinia pseudotuberculosis required for systemic infection.

Virulence 2018 Nov 29. Epub 2018 Nov 29.

a Department of Molecular Biology, Umeå Centre for Microbial Research UCMR , Umeå University , Umeå , Sweden.

Type III secretion systems (T3SSs) are tightly regulated key virulence mechanisms shared by many Gram-negative pathogens. YopN, one of the substrates, is also crucial in regulation of expression, secretion and activation of the T3SS of pathogenic Yersinia species. Interestingly, YopN itself is also targeted into host cells but so far no activity or direct role for YopN inside host cells has been described. Read More

View Article
November 2018

Antibiotic resistant zoonotic bacteria in Irrawaddy squirrel (Callosciurus pygerythrus).

Vet Med Sci 2018 Nov 29. Epub 2018 Nov 29.

Department of Microbiology and Veterinary Public Health, Chittagong Veterinary and Animal Sciences University (CVASU), Chittagong, Bangladesh.

Irrawaddy squirrel (Callosciurus pygerythrus) may play an important role in the transmission of zoonotic bacteria, but little is known about the carriage of zoonotic bacteria in this common frugivorous rodent in Bangladesh. We aimed to investigate the presence of common zoonotic bacterial pathogens in Irrawaddy squirrel in the southeast part of Bangladesh. A total of 27 rectal and 27 oro-nasal swabs were collected from 27 healthy wild Irrawaddy squirrels. Read More

View Article
November 2018
3 Reads

Insecticidal Toxicity of Involves the Novel Enterotoxin YacT.

Front Cell Infect Microbiol 2018 14;8:392. Epub 2018 Nov 14.

Lehrstuhl für Mikrobielle Ökologie, Fakultät für Grundlagen der Biowissenschaften, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany.

The genus comprises 19 species of which three are known as human and animal pathogens. Some species display toxicity toward invertebrates using the so-called toxin complex (TC) and/or determinants that are not yet known. Recent studies showed a remarkable variability of insecticidal activities when representatives of different species (spp. Read More

View Article
November 2018
3 Reads

Integrative approach using genomes to revisit the historical landscape of plague during the Medieval Period.

Proc Natl Acad Sci U S A 2018 Nov 26. Epub 2018 Nov 26.

Centre for Ecological and Evolutionary Synthesis (CEES), Department of Biosciences, University of Oslo, N-0316 Oslo, Norway;

Over the last few years, genomic studies on , the causative agent of all known plague epidemics, have considerably increased in numbers, spanning a period of about 5,000 y. Nonetheless, questions concerning historical reservoirs and routes of transmission remain open. Here, we present and describe five genomes from the second half of the 14th century and reconstruct the evolutionary history of by reanalyzing previously published genomes and by building a comprehensive phylogeny focused on strains attributed to the Second Plague Pandemic (14th to 18th century). Read More

View Article
November 2018
1 Read

Humoral and cellular immune correlates of protection against bubonic plague by a live Yersinia pseudotuberculosis vaccine.

Vaccine 2019 Jan 19;37(1):123-129. Epub 2018 Nov 19.

Unité de Recherche Yersinia, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France. Electronic address:

Immunization with the live-attenuated Yersinia pseudotuberculosis VTnF1 strain producing a Yersinia pestis F1 pseudocapsule efficiently protects mice against bubonic and pneumonic plague. In clinical trials, demonstration of a plague vaccine's efficacy in humans will not be feasible, and correlates of protection will be needed to bridge the immune response of protected animals to that of vaccinated humans. Using serum transfer and vaccination of antibody-deficient µMT mice, we established that both humoral and cellular responses elicited by VTnF1 independently conferred protection against bubonic plague. Read More

View Article
January 2019
4 Reads

Discovering RNA-Based Regulatory Systems for Virulence.

Front Cell Infect Microbiol 2018 25;8:378. Epub 2018 Oct 25.

Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.

The genus includes three human pathogenic species, , the causative agent of the bubonic and pneumonic plague, and enteric pathogens and that cause a number of gut-associated diseases. Over the past years a large repertoire of RNA-based regulatory systems has been discovered in these pathogens using different RNA-seq based approaches. Among them are several conserved or species-specific RNA-binding proteins, regulatory and sensory RNAs as well as various RNA-degrading enzymes. Read More

View Article
October 2018
1 Read

pYR4 From a Norwegian Isolate of Is a Putative Virulence Plasmid Encoding Both a Type IV Pilus and a Type IV Secretion System.

Front Cell Infect Microbiol 2018 30;8:373. Epub 2018 Oct 30.

Department of Biosciences, University of Oslo, Oslo, Norway.

Enteric redmouth disease caused by the pathogen is a significant problem for fish farming around the world. Despite its importance, only a few virulence factors of have been identified and studied in detail. Here, we report and analyze the complete DNA sequence of pYR4, a plasmid from a highly pathogenic Norwegian isolate, sequenced using PacBio SMRT technology. Read More

View Article
October 2018
3 Reads

Microbiological Safety of Fresh Tilapia ( Oreochromis niloticus) from Kenyan Fresh Water Fish Value Chains.

J Food Prot 2018 Dec;81(12):1973-1981

4 Division for Epidemiology and Microbial Genomics, National Food Institute, Technical University of Denmark, 2800 Kongens, Lyngby, Denmark.

This study evaluated the microbiological safety of fresh Nile tilapia ( Oreochromis niloticus) from Kenyan fresh water fish value chains. One hundred seventy-six fish samples were analyzed. The microbial counts of hygiene indicators, total viable aerobic count (TVC), total coliforms, and fecal coliforms isolated by using culture techniques were enumerated, and microbial pathogens present in the fish samples were identified and characterized by using molecular methods. Read More

View Article
December 2018
8 Reads

Metagenomics of pasteurized and unpasteurized gouda cheese using targeted 16S rDNA sequencing.

BMC Microbiol 2018 Nov 19;18(1):189. Epub 2018 Nov 19.

Division of Food Processing Science and Technology, Office of Food Safety, U. S. Food and Drug Administration, Bedford Park, IL, USA.

Background: The microbiome of cheese is diverse, even within a variety. The metagenomics of cheese is dependent on a vast array of biotic and abiotic factors. Biotic factors include the population of microbiota and their resulting cellular metabolism. Read More

View Article
November 2018
3 Reads

Sequence variants of Yersinia enterocolitica ystB gene detected in wild animals in Poland.

Pol J Vet Sci 2018 Jun;21(2):397-399

Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland.

The purpose of the study was to analyze a part of the nucleotide sequences of ystB gene Y. enterocolitica strains isolated from wild animals. The material for the study consists of 30 Y. Read More

View Article

Listeriolysin O-dependent host surfaceome remodeling modulates Listeria monocytogenes invasion.

Pathog Dis 2018 Nov 1;76(8). Epub 2018 Nov 1.

Institut Pasteur, Unité des Interactions Bactéries Cellules, Paris F-75015, France.

Listeria monocytogenes is a pathogenic bacterium that invades epithelial cells by activating host signaling cascades, which promote bacterial engulfment within a phagosome. The pore-forming toxin listeriolysin O (LLO), which is required for bacteria phagosomal escape, has also been associated with the activation of several signaling pathways when secreted by extracellular bacteria, including Ca2+ influx and promotion of L. monocytogenes entry. Read More

View Article
November 2018
1 Read

PGE2 Augments Inflammasome Activation and M1 Polarization in Macrophages Infected With Typhimurium and .

Front Microbiol 2018 31;9:2447. Epub 2018 Oct 31.

Department of Microbiology and Cell Science, College of Agricultural and Life Sciences, University of Florida, Gainesville, FL, United States.

Eicosanoids are cellular metabolites, which shape the immune response, including inflammatory processes in macrophages. The effects of these lipid mediators on inflammation and bacterial pathogenesis are not clearly understood. Certain eicosanoids are suspected to act as molecular sensors for the recruitment of neutrophils, while others regulate bacterial uptake. Read More

View Article
October 2018
1 Read

Succession and persistence of microbial communities and antimicrobial resistance genes associated with International Space Station environmental surfaces.

Microbiome 2018 Nov 13;6(1):204. Epub 2018 Nov 13.

Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Dr, Pasadena, CA, 91109, USA.

Background: The International Space Station (ISS) is an ideal test bed for studying the effects of microbial persistence and succession on a closed system during long space flight. Culture-based analyses, targeted gene-based amplicon sequencing (bacteriome, mycobiome, and resistome), and shotgun metagenomics approaches have previously been performed on ISS environmental sample sets using whole genome amplification (WGA). However, this is the first study reporting on the metagenomes sampled from ISS environmental surfaces without the use of WGA. Read More

View Article
November 2018
4 Reads

Effect of immunostimulatory feed supplements on the development of acquired immunity in rainbow trout (Oncorhynchus mykiss).

Fish Shellfish Immunol 2018 Nov 8;86:1-3. Epub 2018 Nov 8.

Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria.

Immunostimulatory feed supplements are an increasingly common feature of aquaculture management and their benefit has been confirmed for a wide area of products. However, these investigations have often focused on the benefit of these supplements on the innate immune system. In the current project, we investigated a mixture of two commercial feed supplements (Biotronic Top 3 and Levabon Aquagrow E) with a known protective effect against bacterial infections. Read More

View Article
November 2018
5 Reads

A novel chemical lysis method for maximum release of DNA from difficult-to-lyse bacteria.

Microb Pathog 2018 Nov 8;126:292-297. Epub 2018 Nov 8.

DNA Genotek Inc., 3000 - 500 Palladium Drive, Ottawa, ON, K2V 1C2, Canada.

Molecular detection of microorganisms requires releasing DNA from cells. However, since certain microbial organisms are refractory to lysis by chemical or enzymatic methods, mechanical lysis by bead-beating is typically employed to disrupt difficult-to-lyse microbes. A newly developed chemical lysis method called sporeLYSE enables release of DNA from difficult-to-lyse microbes without bead-beating. Read More

View Article
November 2018
13 Reads

Interrelationships between Multiple Climatic Factors and Incidence of Foodborne Diseases.

Int J Environ Res Public Health 2018 Nov 7;15(11). Epub 2018 Nov 7.

Department of Food and Nutrition, Kunsan National University, Gunsan 54150, Jeonbuk, Korea.

Climatic factors can affect the incidence of foodborne diseases (FBDs). Moreover, microbial network inference is useful for predicting the interrelationships between the incidence of FBDs and climatic factors. However, the interrelationships between FBD pathogens and most climatic factors are unknown. Read More

View Article
November 2018
6 Reads

Prevalence and Antimicrobial Resistance of Escherichia coli, Enterococcus spp. and the Major Foodborne Pathogens in Calves in Latvia.

Foodborne Pathog Dis 2018 Nov 7. Epub 2018 Nov 7.

1 Institute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies , Jelgava, Latvia .

The aim of the present study was to detect the prevalence and antimicrobial resistance of fecal indicators and major foodborne pathogens in feces of calves and to identify the factors associated with increased prevalence of resistant bacteria on farms. Altogether, 180 rectal swabs were collected from 18 farms in Latvia. Samples were investigated to detect the prevalence and antimicrobial resistance of Escherichia coli, Enterococcus spp. Read More

View Article
November 2018
6 Reads

Flagellar motility plays important role in Biofilm formation of Bacillus cereus and Yersinia enterocolitica.

Pak J Pharm Sci 2018 Sep;31(5(Supplementary)):2047-2052

Department of Zoology, Govt. College University, Lahore, Pakistan.

Bacteria live either independently as planktonic cells or in organized surface associated colonies called as biofilms. Biofilms play an important role in increased pathogenesis of bacteria and it is assumed that motility is one of the contributing factors towards biofilm initiation. This study was planned to identify the role of flagella in biofilm formation by constructing flagellated (wild type) and physically disrupted variants (non-motile). Read More

View Article
September 2018
2 Reads

Gastrointestinal Infection Increases Odds of Inflammatory Bowel Disease in a Nationwide Case-Control Study.

Clin Gastroenterol Hepatol 2018 Oct 30. Epub 2018 Oct 30.

Division of Digestive and Liver Disease, Department of Medicine, Columbia University Medical Center, New York, New York; Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Solna, Sweden; Department of Pediatrics, Orebro University Hospital, Orebro, Sweden; Division of Epidemiology and Public Health, School of Medicine, University of Nottingham, UK.

Background & Aims: Gastrointestinal infections have been associated with later development of inflammatory bowel diseases (IBD). However, studies have produced conflicting results. We performed a nationwide case-control study in Sweden to determine whether gastroenteritis is associated with the development of Crohn's disease (CD) or ulcerative colitis (UC). Read More

View Article
October 2018
7 Reads

Structure and gene cluster of the O-polysaccharide of Yersinia rohdei H274-36/78.

Int J Biol Macromol 2018 Oct 29;122:555-561. Epub 2018 Oct 29.

State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Moscow Region, Russian Federation.

A branched O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Yersinia rohdei H274-36/78 and found to contain d-rhamnose, d-mannose, and 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-d-xylo-hexose called yersiniose A (Yer). Partial acid hydrolysis of the O-polysaccharide eliminated Yer residues to give a modified linear polysaccharide. Studies by sugar analysis and H and C NMR spectroscopy, including computational NMR analysis, enabled structure elucidation of a hexasaccharide repeating unit of the O-polysaccharide having two Yer residues attached as monosaccharide side chains. Read More

View Article
October 2018

Caspase-8 induces cleavage of gasdermin D to elicit pyroptosis during infection.

Proc Natl Acad Sci U S A 2018 Nov 31;115(46):E10888-E10897. Epub 2018 Oct 31.

Department of Immunology, Tufts University School of Medicine, Boston, MA 02111;

Cell death and inflammation are intimately linked during infection. Pathogenic inhibits the MAP kinase TGFβ-activated kinase 1 (TAK1) via the effector YopJ, thereby silencing cytokine expression while activating caspase-8-mediated cell death. Here, using in corroboration with costimulation of lipopolysaccharide and (5Z)-7-Oxozeaenol, a small-molecule inhibitor of TAK1, we show that caspase-8 activation during TAK1 inhibition results in cleavage of both gasdermin D (GSDMD) and gasdermin E (GSDME) in murine macrophages, resulting in pyroptosis. Read More

View Article
November 2018
6 Reads

Targeting of the F1 capsular antigen by innate-like B1b cells mediates a rapid protective response against bubonic plague.

NPJ Vaccines 2018 19;3:52. Epub 2018 Oct 19.

1Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, P.O. Box 19, Ness-Ziona, 74100 Israel.

The generation of adaptive immunity by vaccination is usually a prolonged process that requires multiple dosing over several months. Hence, vaccines are administered for disease prevention a relatively long time prior to possible infection as opposed to post-exposure prophylaxis, which typically requires rapid intervention such as antibiotic therapy. The emergence of pathogens resistant to common antibiotic treatments has prompted the search for alternative therapeutic strategies. Read More

View Article
October 2018
4 Reads

Chemical composition of Acacia farnesiana (L) wild fruits and its activity against Mycobacterium tuberculosis and dysentery bacteria.

J Ethnopharmacol 2019 Feb 25;230:74-80. Epub 2018 Oct 25.

Universidad Autónoma de Nuevo León, Facultad de Ciencias Químicas, Av. Universidad S/N, Ciudad Universitaria, CP 66451 San Nicolás de los Garza, Nuevo León, Mexico. Electronic address:

Ethnopharmacological Relevance: In Mexico, plants are an important element of traditional medicine, and many are considered part of Mexican cultural heritage from prehispanic and colonial times. Nevertheless, relatively few systematic scientific studies have been conducted to fully characterize the chemical composition and pharmacological activities of Mexican medicinal plants. Acacia farnesiana is used in Mexican traditional medicine to treat dysentery and tuberculosis and therefore could have bioactive compounds that may explain its traditional use. Read More

View Article
February 2019
11 Reads

Rapid detection of Yersinia enterocolitica serotype O:3 using a duplex PCR assay.

J Microbiol Methods 2018 Nov 23;154:107-111. Epub 2018 Oct 23.

Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria, Rio de Janeiro /RJ, Brazil.

Yersinia enterocolitica, a member of the Enterobacteriaceae family, is a zoonotic agent that causes gastrointestinal diseases and some extraintestinal disorders in humans. Y. enterocolitica ssp. Read More

View Article
November 2018
2 Reads

Pathogen blockade of TAK1 triggers caspase-8-dependent cleavage of gasdermin D and cell death.

Science 2018 Nov 25;362(6418):1064-1069. Epub 2018 Oct 25.

Program in Innate Immunity, Department of Medicine, Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01605, USA.

Limited proteolysis of gasdermin D (GSDMD) generates an N-terminal pore-forming fragment that controls pyroptosis in macrophages. GSDMD is processed via inflammasome-activated caspase-1 or -11. It is currently unknown whether macrophage GSDMD can be processed by other mechanisms. Read More

View Article
November 2018
31.480 Impact Factor

Systemic Yersinia pseudotuberculosis as a Cause of Osteomyelitis in a Captive Ring-tailed Lemur (Lemur catta).

J Comp Pathol 2018 Oct 11;164:27-31. Epub 2018 Sep 11.

Royal (Dick) School of Veterinary Studies, The Roslin Institute, University of Edinburgh, Easter Bush, Edinburgh, UK.

Yersinia pseudotuberculosis and Yersinia enterocolitica are ubiquitous pathogens with wildlife and domestic animal reservoirs. Outbreaks of 'non-plague' yersiniosis in man and non-human primates are reported frequently (including zoological specimens and research breeding colonies) and are usually characterized by enteritis, mesenteric lymphadenitis and occasionally organ abscessation. In people, non-septic reactive arthritis is a common sequela to yersiniosis. Read More

View Article
October 2018
1 Read

YbtT is a low-specificity type II thioesterase that maintains production of the metallophore yersiniabactin in pathogenic enterobacteria.

J Biol Chem 2018 Oct 24. Epub 2018 Oct 24.

Washington University, United States.

Clinical isolates of , , and frequently secrete the small molecule metallophore yersiniabactin (Ybt), which passivates and scavenges transition metals during human infections. YbtT is encoded within the Ybt biosynthetic operon and is critical for full Ybt production in bacteria. However, its biosynthetic function has been unclear because it is not essential for Ybt production by the reconstituted nonribosomal peptide synthetase/polyketide synthase (NRPS/PKS) pathway. Read More

View Article
October 2018
3 Reads

Diversity of Yersinia enterocolitica isolated from pigs in a French slaughterhouse over 2 years.

Microbiologyopen 2018 Oct 22:e751. Epub 2018 Oct 22.

Hygiene and Quality of Poultry and Pig Products Unit, Ploufragan/Plouzané Laboratory, ANSES, University of Bretagne-Loire, Ploufragan, France.

The pig is one of the main reservoirs of Yersinia enterocolitica strains pathogenic to humans. A description of the Y. enterocolitica population in this reservoir, and accurate discriminatory techniques for typing isolates are needed for prevention, outbreak investigation, and surveillance. Read More

View Article
October 2018

exploits CD209 receptors for promoting host dissemination and infection.

Infect Immun 2018 Oct 22. Epub 2018 Oct 22.

Department of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan, Hubei, China

is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen and liver of infected humans and animals. Although the molecular mechanisms for dissemination and infection are unclear, many Gram-negative enteropathogens presumably invade into the small intestine via the Peyer's patches to initiate dissemination. Read More

View Article
October 2018
5 Reads
3.730 Impact Factor

Effects of aeration on microbes and intestinal bacteria in bioaerosols from the BRT of an indoor wastewater treatment facility.

Sci Total Environ 2019 Jan 19;648:1453-1461. Epub 2018 Aug 19.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; National Engineering Laboratory for VOCs Pollution Control Material & Technology, University of Chinese Academy of Sciences, Beijing 101408, China. Electronic address:

The generation and emission of airborne bacteria from a biochemical reaction tank (BRT) for wastewater treatment was investigated by altering the aeration rate. The levels of bioaerosols increased from 715 ± 69 to 1597 ± 135 CFU/m (total airborne bacteria) and from 78 ± 6 to 359 ± 18 CFU/m (intestinal bacteria) as the aeration rate increased from 0.3 to 1. Read More

View Article
January 2019

Examination of the hygienic status of selected organic enrichment materials used in pig farming with special emphasis on pathogenic bacteria.

Porcine Health Manag 2018 11;4:24. Epub 2018 Oct 11.

Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour (ITTN), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15 (Building 116), 30173 Hannover, Germany.

Background: Enrichment materials for pigs, particularly organic materials, are becoming increasingly important in order to reduce abnormal behaviour such as tail biting. However, potential health risks posed by these materials (such as the introduction of pathogens into the herd) have not been sufficiently studied to date. Therefore, 21 different organic materials used as enrichment materials in pig farming were tested for total viable count of mesophilic bacteria, moulds, coliforms, , spp. Read More

View Article
October 2018
3 Reads

BfvR, an AraC-Family Regulator, Controls Biofilm Formation and pH6 Antigen Production in Opposite Ways in Biovar Microtus.

Front Cell Infect Microbiol 2018 2;8:347. Epub 2018 Oct 2.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.

Biofilm formation is critical for blocking flea foregut and hence for transmission of by flea biting. In this study, we identified the regulatory role of the AraC-family transcriptional regulator BfvR (YPO1737 in strain CO92) in biofilm formation and virulence of biovar Microtus. Crystal violet staining, biofilm assay, colony morphology assay, intracellular c-di-GMP concentration determination, and BALB/c mice challenge were employed to reveal that BfvR enhanced biofilm formation while repressed its virulence in mice. Read More

View Article
October 2018
2.620 Impact Factor

A Bacteriophage T4 Nanoparticle-Based Dual Vaccine against Anthrax and Plague.

MBio 2018 Oct 16;9(5). Epub 2018 Oct 16.

Department of Biology, The Catholic University of America, Washington, DC, USA

and , the causative agents of anthrax and plague, respectively, are two of the deadliest pathogenic bacteria that have been used as biological warfare agents. Although Biothrax is a licensed vaccine against anthrax, no Food and Drug Administration-approved vaccine exists for plague. Here, we report the development of a dual anthrax-plague nanoparticle vaccine employing bacteriophage (phage) T4 as a platform. Read More

View Article
October 2018
1 Read

First use of whole-genome sequencing to investigate a cluster of Yersinia enterocolitica, Liverpool, United Kingdom, 2017.

J Med Microbiol 2018 Dec 16;67(12):1747-1752. Epub 2018 Oct 16.

4​North West Health Protection Team, Public Health England, Liverpool, UK.

Purpose: In May 2017 we were notified of a cluster of Yersinia enterocolitica-positive isolates from Liverpool. The purpose of this work was to investigate this cluster of cases and find a possible common source. We combined epidemiological information with whole-genome sequencing (WGS) results, which indicated that these cases were unlikely to be from the same source. Read More

View Article
December 2018

Suspicious granulomatous enterocolitis mimicking appendicitis.

BMJ Case Rep 2018 Oct 14;2018. Epub 2018 Oct 14.

General Surgery, Royal Bolton Hospital, Bolton NHS Foundation Trust, Bolton, UK.

We present the case of a fit and well 12-year-old boy who presented with signs and symptoms suggestive of appendicitis. On laparoscopy, he was found to have a sinister-looking right iliac fossa mass with associated mesenteric lymphadenopathy. He proceeded to have an oncological right hemicolectomy, while the subsequent histology returned an unexpected result. Read More

View Article
October 2018
1 Read

Targeting peptide-enhanced antibody and CD11c dendritic cells to inclusion bodies expressing protective antigen against ETEC in mice.

FASEB J 2018 Oct 15:fj201800289RRR. Epub 2018 Oct 15.

Key Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China.

Enterotoxigenic Escherichia coli (ETEC) remains a massive burden in developing countries with increasing morbidity and mortality rates; it is also an important pathogen in the farming industry and is a leading cause of bacterial diarrhea. Our previous study showed that nanometer-sized inclusion bodies (IBs) of the fimbrial adhesin subunit protein (FaeG), mutation heat-stable enterotoxin a (mSTa), heat-labile enterotoxin b (LTb), and STb (nontargeting) fusion protein as an oral vaccine induced both systemic and mucosal immune responses. In this study, to enhance the protective efficacy to ETEC, we used Yersinia enterocolitica adhesive and M-cell-targeting peptides to analyze high-efficiency antigen-specific immune presentation in the gut. Read More

View Article
October 2018
7 Reads

Author Correction: Proteome analysis reveals a role of rainbow trout lymphoid organs during Yersinia ruckeri infection process.

Sci Rep 2018 Oct 12;8(1):15332. Epub 2018 Oct 12.

Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. Read More

View Article
October 2018

M cell-targeting strategy enhances systemic and mucosal immune responses induced by oral administration of nuclease-producing L. lactis.

Appl Microbiol Biotechnol 2018 Dec 11;102(24):10703-10711. Epub 2018 Oct 11.

Department of Microbiology and Immunology, Gifu Pharmaceutical University, 1-25-4 Daigaku Nishi, Gifu, 501-1196, Japan.

Efficient delivery of antigens to the gut-associated lymphoid tissue (GALT) is the most critical step for the induction of mucosal immunity by oral vaccines. As M cells are the main portal for luminal antigens into the GALT, the M cell-targeting of antigens affords a promising strategy toward the development of effective oral vaccines. Lactococcus lactis is a fascinating recombinant host for oral vaccines, as they survive and produce antigens in the gut and have a particularly safe profile for human use. Read More

View Article
December 2018
10 Reads
3.340 Impact Factor

Ongoing quiescence in the Borborema Plateau Plague focus (Paraiba, Brazil).

An Acad Bras Cienc 2018 Jul-Sep;90(3):3007-3015

Programa de Pós-Graduação em Ciências Biológicas, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, Cidade Universitária, s/n, Castelo Branco, 58051-900 João Pessoa, PB, Brazil.

Plague is a zoonosis caused by Yersinia pestis, whose cycle is based on a reservoir system composed of mammals and their fleas. Its transmission cycle presents long enzootic periods with undetected cases, sometimes misleading that the cycle is extinct. While surveillance activities in Brazil are being carried out only in some focal areas, the serologic results confirm the persistence of Y. Read More

View Article
December 2017
2 Reads

Intrinsic Parameters and Bacterial Growth Prediction in a Brazilian Minimally Ripened Cheese (Coalho) during Refrigerated Storage.

J Food Prot 2018 Nov;81(11):1800-1809

1 Laboratório de Microbiologia de Alimentos, Departamento de Nutrição, Universidade Federal da Paraíba, João Pessoa, Paraíba, 58051-900 Brazil.

This study evaluated the microbiological and physicochemical characteristics in different commercial brands of a Brazilian minimally ripened (coalho) cheese during 60 days of storage under refrigeration. Combinations of maximum and minimum values of water activity and pH determined in cheese samples at refrigeration temperature (7°C) were used in a bacterial growth prediction analysis. Maximum growth rate (Grmax) was estimated for different pathogenic and/or spoilage bacteria using the ComBase Predictor. Read More

View Article
November 2018
1 Read

Prevalence of Species in the Ileum of Crohn's Disease Patients and Controls.

Front Cell Infect Microbiol 2018 21;8:336. Epub 2018 Sep 21.

UMR1149 INSERM, Research Centre on Inflammation, Université Paris Diderot-Sorbonne Paris-Cité, Paris, France.

are common contaminants of food products, but their prevalence in the human gut is poorly documented. have been implicated in Crohn's Disease (CD, an inflammatory bowel disease) however their role in CD is controversial. We performed highly sensitive PCR assays of specific sequences for the gene of and the gene of . Read More

View Article
September 2018
3 Reads
2.620 Impact Factor