22,896 results match your criteria Wound Healing Growth Factors


The effect of methacrylic acid in smooth coatings on dTHP1 and HUVEC gene expression.

Biomater Sci 2014 Dec 6;2(12):1768-1778. Epub 2014 Aug 6.

Institute of Biomaterials and Biomedical Engineering, University of Toronto, Canada.

Without any external additives such as growth factors, polymer beads containing methacrylic acid (MAA) promoted functional vascularization in vivo leading to faster cutaneous wound healing in diabetic mice and improved skin graft integration in Wistar rats. The aim of this work is to understand this material-driven vascularization by investigating the effect of polymer MAA-content, in the absence of surface roughness, on the behaviour of macrophage-like and endothelial cells. Smooth polymer films containing 20, 30 or 40% MAA or methyl methacrylate as a control copolymerized with isodecyl acrylate, were synthesized to study the effect of MAA content in smooth films, without roughness. Read More

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http://dx.doi.org/10.1039/c4bm00159aDOI Listing
December 2014

Role and effect of vein-transplanted human umbilical cord mesenchymal stem cells in the repair of diabetic foot ulcers in rats.

Acta Biochim Biophys Sin (Shanghai) 2020 Jun 2. Epub 2020 Jun 2.

Department of Interventional Radiology, Affiliated Hospital of Nantong University, Nantong 226001, China.

Diabetic foot ulcer (DFU) is one of diabetic complications, which is frequently present and tormented in diabetes mellitus. Most multipotent mesenchymal stromal cells (MSCs) are capable of immune evasion, providing an allogeneic, ready-to-use, cell product option for therapeutic applications. The beneficial effect of MSCs for the treatment of a variety of traumatic injuries, such as open wounds, has been extensively explored. Read More

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http://dx.doi.org/10.1093/abbs/gmaa039DOI Listing

Pre-mRNA processing factor 3 enhances the progression of keratinocyte-derived cutaneous squamous cell carcinoma by regulating the JAK2/STAT3 pathway.

Sci Rep 2020 Jun 1;10(1):8863. Epub 2020 Jun 1.

Department of Dermatology and Venereology, The First Hospital of Jilin University, Changchun, China.

The precise role of pre-mRNA processing factors (PRPs) in human tumorigenesis has not been yet explored. The object of the present study was to explore the effects of PRP3 in a common metastatic skin cancer, keratinocyte-derived cutaneous squamous cell carcinoma (cSCCs). RT-qPCR and western blotting were conducted to measure the expression levels of PRP3 in various cSCC cell lines and cSCC tissues. Read More

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http://dx.doi.org/10.1038/s41598-020-65928-8DOI Listing

[Etiopathogenetic factors of wound healing in chronic post-intubation cicatricial stenosis of the larynx and trachea].

Vestn Otorinolaringol 2020 ;85(2):78-83

National Medical Research Center for Otorhinolaryngology of the FMBA of Russia, Moscow, Russia.

Despite the existing modern high-tech methods of examination and a variety of surgical treatment methods, the problem of diagnosis, treatment and rehabilitation of patients with chronic post-intubation cicatricial stenosis of the larynx and trachea still requires further study. Improving the understanding and correction of cellular, molecular genetic and biochemical disorders in a chronic wound is a key condition for increasing the efficiency of diagnosis, individual prognosis of the clinical course and the conduct of adequate therapeutic and preventive measures for post-intubation cicatricial laryngotracheal stenoses. In this regard, it seemed appropriate to analyze the existing etiopathogenetic factors of pathological wound healing in chronic post-intubation cicatricial stenosis of the larynx and trachea. Read More

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http://dx.doi.org/10.17116/otorino20208502178DOI Listing
January 2020

Platelet-rich plasma for laryngotracheal reconstruction: an experimental study.

Eur Arch Otorhinolaryngol 2020 May 31. Epub 2020 May 31.

Department of Otolaryngology, Uskudar Hospitalturk Hospital, Istanbul, Turkey.

Objectives/hypothesis: This study was designed to evaluate the graft healing effect of topical application of platelet-rich plasma (PRP) for laryngotracheal reconstruction (LTR) in a rabbit model.

Study Design: It is a prospective randomized control animal study.

Materials And Methods: Sixteen healthy New Zealand White rabbits were assigned to two groups of eight animals each. Read More

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http://dx.doi.org/10.1007/s00405-020-06091-8DOI Listing

Electroceutical Management of Bacterial Biofilm and Surgical Infection.

Antioxid Redox Signal 2020 May 29. Epub 2020 May 29.

Indiana University School of Medicine, 12250, Clinical Research IUH Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering (ICRME), 444A MRLB, 975 Walnut Street, Indianapolis, Indiana, United States, 46202-5114;

Significance: In the host-microbe microenvironment, bioelectrical factors influence microbial, host as well as host-microbe interactions. This article discusses relevant mechanistic underpinnings of this novel paradigm. It also addresses how such knowledge may be leveraged to develop novel electroceutical solutions to manage biofilms in the context of surgical infection. Read More

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http://dx.doi.org/10.1089/ars.2020.8086DOI Listing

Accelerated healing by topical administration of Salvia officinalis essential oil on Pseudomonas aeruginosa and Staphylococcus aureus infected wound model.

Biomed Pharmacother 2020 May 24;128:110120. Epub 2020 May 24.

Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran.

Background: Salvia officinalis L. (Lamiaceae) is known to have antibacterial properties possibly conducive to the healing process of infected wounds.

Purpose: The present study aimed to evaluate the effects of an ointment containing Salvia officinalis essential oil (SOO) on an infected wound model. Read More

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http://dx.doi.org/10.1016/j.biopha.2020.110120DOI Listing

Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care.

J Biomed Mater Res B Appl Biomater 2020 May 26. Epub 2020 May 26.

J. Crayton Pruitt Family Department of Biomedical Engineering , University of Florida, Gainesville, Florida, USA.

There is a growing clinical demand in the wound care market to treat chronic wounds such as diabetic foot ulcers. Advanced cell and tissue-based products (CTPs) are often used to address challenging chronic wounds where healing has stalled. These products contain active biologics such as growth factors and cytokines as well as structural components that support and stimulate cell growth and assist in tissue regeneration. Read More

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http://dx.doi.org/10.1002/jbm.b.34635DOI Listing

YiQi GuBen Formula Inhibits PDGF-BB-Induced Proliferation and Migration of Airway Smooth Muscle Cells.

Pharmacology 2020 May 26:1-10. Epub 2020 May 26.

Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, China,

Objective: Increased proliferation and migration of airway smooth muscle cells (ASMCs) are key events in the development of asthma. YiQi GuBen is a traditional Chinese medicinal formula shown to effectively reduce the recurrence rate of asthma and induce anti-asthma effects through multiple pathways; however, its potential role in regulating ASMC proliferation and preventing bronchial asthma remains unexplored.

Methods: This study investigated the effects of YiQi GuBen formula on platelet-derived growth factor (PDGF)-BB-induced ASMC proliferation and migration by methylthiazolyldiphenyl-tetrazolium bromide, wound healing, transwell, and cell cycle assays. Read More

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http://dx.doi.org/10.1159/000504516DOI Listing

Proliferative Status in the Aqueous Humor of Eyes With Congenital Cataract.

J Pediatr Ophthalmol Strabismus 2020 May;57(3):159-168

Purpose: To measure the concentrations of growth factors in the aqueous humor of patients with congenital cataract and to investigate the biological effects of a selected cytokine (fibroblast growth factor 4 [FGF4]) on cell proliferation, migration, and transformation.

Methods: In the aqueous humor obtained from 55 eyes with congenital cataract and 55 eyes with age-related cataract, 40 growth factors were screened and selected cytokines were confirmed with enzyme-linked immunosorbent assays. After the addition of various concentrations of FGF4 (0, 2. Read More

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http://dx.doi.org/10.3928/01913913-20200224-01DOI Listing

Downregulation of PHF19 inhibits cell growth and migration in gastric cancer.

Scand J Gastroenterol 2020 May 23:1-7. Epub 2020 May 23.

Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

The PHD Finger Protein 19 (PHF19), as a sub-component of polycomb repressive complex 2 (PRC2), has been identified to be associated with various biological processes. Aberrant expression of PHF19 has implicated in several cancer types. This study aims to investigate its function and clinical significance in gastric cancer for the first time. Read More

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http://dx.doi.org/10.1080/00365521.2020.1766555DOI Listing

Platelet-rich fibrin and concentrated growth factors as novel platelet concentrates for chronic hard-to-heal skin ulcers: a systematic review and Meta-analysis of randomized controlled trials.

J Dermatolog Treat 2020 Jun 1:1-9. Epub 2020 Jun 1.

Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

This study aimed to determine superior outcomes of platelet-rich fibrin and concentrated growth factors dressings in chronic hard-to-heal skin ulcers. A search in PubMed, EMBASE, Cochrane Library and CINAHL(EBSCO) was performed for randomized controlled trials comparing platelet-rich fibrin or concentrated growth factors dressings to standard wound care in chronic hard-to-heal skin ulcers. Primary outcome was the number of ulcers completely healed. Read More

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http://dx.doi.org/10.1080/09546634.2020.1773386DOI Listing

Evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients.

Regen Ther 2020 Jun 15;14:222-226. Epub 2020 May 15.

Department of Plastic and Reconstructive Surgery, International University of Health and Welfare, School of Medicine, 4-3, Kozunomori, Narita, Chiba, 286-8686, Japan.

Impaired wound healing in critical limb ischemia (CLI) results from multiple factors that affect many cell types and their behavior. Epidermal keratinocytes and dermal fibroblasts play crucial roles in wound healing. However, it remains unclear whether these cell types irreversibly convert into a non-proliferative phenotype and are involved in impaired wound healing in CLI. Read More

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http://dx.doi.org/10.1016/j.reth.2020.03.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229408PMC

Sequential Delivery of Cryogel Released Growth Factors and Cytokines Accelerates Wound Healing and Improves Tissue Regeneration.

Front Bioeng Biotechnol 2020 17;8:345. Epub 2020 Apr 17.

Department of Medicine, School of Medicine, Nazarbayev University, Nur-Sultan, Kazakhstan.

Growth factors and cytokines that are secreted by cells play a crucial role in the complex physiological reaction to tissue injury. The ability to spatially and temporally control their actions to maximize regenerative benefits and minimize side effects will help accelerate wound healing and improve tissue regeneration. In this study, the sequential targeted delivery of growth factor/cytokine combinations with regulatory functions on inflammation and tissue regeneration was examined using an internal splint wound healing model. Read More

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http://dx.doi.org/10.3389/fbioe.2020.00345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212449PMC

Biological responses induced by high molecular weight chitosan administrated jointly with Platelet-derived Growth Factors in different mammalian cell lines.

Int J Biol Macromol 2020 May 8;158:953-967. Epub 2020 May 8.

Departamento de Química Biológica, Laboratorio Interdisciplinario de Dinámica Celular y Nanoherramientas, CONICET-Universidad de Buenos Aires, Instituto de Química Biológica Ciencias Exactas y Naturales (IQUIBICEN), Facultad de Ciencias Exactas y Naturales, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina. Electronic address:

In this work, we studied cellular responses known to be involved in tissue regeneration, such as proliferation, migration and tubulogenesis under High Molecular Weight Chitosan (HMWC) and recombinant Platelet-derived Growth Factor (PDGF) treatments using an in vitro cell culture approach. We also analysed changes in mitochondrial dynamics that could be associated with such biological responses. For this proposes, endothelial human cell lines (EA. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.032DOI Listing

Increased Level of Vascular Endothelial Growth Factors by 4-hexylresorcinol is Mediated by Transforming Growth Factor-β1 and Accelerates Capillary Regeneration in the Burns in Diabetic Animals.

Int J Mol Sci 2020 May 14;21(10). Epub 2020 May 14.

Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung 28644, Korea.

4-Hexyl resorcinol (4HR) is an organic compound and has been used in skin care application. 4HR is an M2-type macrophage activator and elevates vascular endothelial growth factor (VEGF) expression via the hypoxia-inducible factor (HIF)-independent pathway. As endothelial cells are important in wound healing, the human umbilical vein endothelial cells (HUVECs) were treated with 4HR, and changes in VEGF-A, -C, and transforming growth factor-β1 (TGF-β1) expression were investigated. Read More

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http://dx.doi.org/10.3390/ijms21103473DOI Listing

Autologous Matrix of Platelet-Rich Fibrin in Wound Care Settings: A Systematic Review of Randomized Clinical Trials.

J Funct Biomater 2020 May 14;11(2). Epub 2020 May 14.

Graduate Program on Health Technology, Pontifical Catholic University of Paraná, Curitiba, PR 80215-901, Brazil.

Platelet-rich fibrin (PRF) consists of a matrix that provides the necessary elements for wound healing, acting as a biodegradable scaffold for cell migration, proliferation, and differentiation, in addition to the delivery of growth factors and angiogenesis. This study aims to determine the effectiveness of the autologous PRF in the treatment of wounds of different etiologies. We carried out a systematic review of randomized clinical trials, guided by the recommendations of the Cochrane Collaboration using the following databases: Pubmed/MEDLINE, EMBASE, Web of Science, and CENTRAL. Read More

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http://dx.doi.org/10.3390/jfb11020031DOI Listing

The angiogenic effects of exosomes secreted from retinal pigment epithelial cells on endothelial cells.

Biochem Biophys Rep 2020 Jul 11;22:100760. Epub 2020 May 11.

Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto, Japan.

Exosomes are informative microvesicles associated with intercellular communication via the transfer of many molecular constituents such as proteins, lipids, and nucleic acids; environmental changes and the cellular status around cells greatly affect exosome components. Cells of the retinal pigment epithelium (RPE) are key players in retinal homeostasis. Transforming growth factor (TGF)-β and tumour necrosis factor (TNF)-α are increased in the vitreous and retina in several retinal diseases and activate and undergo epithelial-mesenchymal transition (EMT) in RPE cells. Read More

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http://dx.doi.org/10.1016/j.bbrep.2020.100760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218265PMC

Quercetin loaded chitosan tripolyphosphate nanoparticles accelerated cutaneous wound healing in Wistar rats.

Eur J Pharmacol 2020 May 11;880:173172. Epub 2020 May 11.

Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.

Previous studies have shown that quercetin on topical application improved cutaneous wound healing in rats, but hydrophobic nature and less skin penetration limits its potential as topical healing agent. Therefore, present study was planned to investigate wound healing potential of chitosan based quercetin nanoparticles. Quercetin loaded nanoparticles were synthesized by ionic gelation method and characterized by various standard techniques. Read More

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http://dx.doi.org/10.1016/j.ejphar.2020.173172DOI Listing

Wetting properties of blood lipid fractions on different titanium surfaces.

Int J Implant Dent 2020 May 13;6(1):16. Epub 2020 May 13.

Department of Periodontology, Faculty of Dentistry, Istanbul University, İstanbul, Turkey.

Background: Blood is the first tissue contacting the implant surface and starting the biological interactions to enhance osseointegration and stimulate bone formation with the progenitor cytokines, chemokines, and growth factors. The coagulation cascade initiates the first step of osseointegration between implant and neighboring tissues. The wound healing may be inadequate unless the blood wets the implant surface properly. Read More

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http://dx.doi.org/10.1186/s40729-020-00213-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218032PMC

The Role of Adipose-Derived Stem Cells, Dermal Regenerative Templates, and Platelet-Rich Plasma in Tissue Engineering-Based Treatments of Chronic Skin Wounds.

Stem Cells Int 2020 9;2020:7056261. Epub 2020 Jan 9.

Department of Clinic and Experimental Medicine, University of Foggia, Foggia 71122, Italy.

The continuous improvements in the field of both regenerative medicine and tissue engineering have allowed the design of new and more efficacious strategies for the treatment of chronic or hard-to-heal skin wounds, which represent heavy burden, from a medical and economic point of view. These novel approaches are based on the usage of three key methodologies: stem cells, growth factors, and biomimetic scaffolds. These days, the adipose tissue can be considered the main source of multipotent mesenchymal stem cells, especially adipose-derived stem cells (ASCs). Read More

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http://dx.doi.org/10.1155/2020/7056261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199611PMC
January 2020

Can Intra-articular 1α, 25-Dihydroxyvitamin D3 Administration Be Therapeutical in Joint Cartilage Damage?

Acta Chir Orthop Traumatol Cech 2020 ;87(2):90-94

Istanbul Medipol University Faculty of Medicine, Department of Orthopedics and Traumatology, Istanbul, Turkey.

INTRODUCTION Vitamin D-deficiency is known to cause nerve conduction impairments, cancer and chronic diseases, as well as the pathogenesis of osteoarthritis. Our goal with this study is to evaluate the cartilage healing by applying intraarticular 1α, 25 (OH) 2D3 at different doses in rats with normal vitamin D levels and metabolism, which we made focal chondral damage model in the knee joint. MATERIAL AND METHODS 35 male Sprague-Dawley rats aged 20-24 weeks were used in our study. Read More

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Platelet-rich plasma loaded with antibiotics as an affiliated treatment for infected bone defect by combining wound healing property and antibacterial activity.

Platelets 2020 May 12:1-13. Epub 2020 May 12.

Department of Emergency Medicine of Daping Hospital, Army Medical University, Chongqing, China.

To be faced with an infected bone defect and the need to accelerate bone union while controlling infection is a welcome challenge for orthopedists. Platelet-rich plasma (PRP) has been applied in tissue defects given their composition of growth factors however the weak antibacterial effects have limited the use of PRP in the clinical setting. Therefore, the aim of this study was to explore the feasibility of using PRP in a local antibiotic delivery system (PADS) with the characteristics of promoting wound healing of bone infection. Read More

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http://dx.doi.org/10.1080/09537104.2020.1759792DOI Listing

Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing.

Int J Biol Macromol 2020 May 6;159:497-509. Epub 2020 May 6.

Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. Electronic address:

In skin tissue engineering, a biodegradable scaffold is usually used where cells grow, produce its own cytokines, growth factors, and extracellular matrix, until the regenerated tissue gradually replaces the scaffold upon its degradation. However, the role of non-biodegradable scaffold remains unexplored. This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.011DOI Listing

Improved growth factor delivery and cellular activity using concentrated platelet-rich fibrin (C-PRF) when compared with traditional injectable (i-PRF) protocols.

Clin Oral Investig 2020 May 7. Epub 2020 May 7.

Department of Periodontology, University of Bern, Bern, Switzerland.

Objectives: Several studies have recently demonstrated that only marginal improvements in platelet and leukocyte concentrations are achieved following standard injectable platelet-rich fibrin (i-PRF) protocols. Due to these previous findings, a novel harvesting technique was recently developed to collect higher concentrations of platelets/leukocytes specifically from the buffy coat layer (C-PRF) following faster centrifugation protocols. The aim of this study was to investigate the regenerative properties and effects on growth factor release and cellular activity of PRF collected through this novel harvesting technique compared to standard i-PRF protocols. Read More

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http://dx.doi.org/10.1007/s00784-020-03303-7DOI Listing

[Administering Ramucirumab Safely after Inserting an Intestinal Stent, Due to Peritoneal Metastasis of Gastric Cancer-A Case Report].

Gan To Kagaku Ryoho 2020 Feb;47(2):313-315

Dept. of Gastrointestinal and Pediatric Surgery, Tokyo Medical University.

The recent phase Ⅲ trials REGARD and RAINBOW have shown survival benefits and acceptable safetyprofiles of ramucirumab( RAM)alone and RAM plus paclitaxel. Based on this result, RAM is recommended as a secondarytreatment for advanced and recurrent gastric cancer bythe Japanese Gastric Cancer Association. Although the frequencyis not high, gastrointestinal perforation has been reported as a serious side effect. Read More

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February 2020

Zinc Oxide Nanoparticles Functionalized on Hydrogel Grafted Silk Fibroin Fabrics as Efficient Composite Dressing.

Biomolecules 2020 May 4;10(5). Epub 2020 May 4.

Department of Chemistry, Amity School of Applied Sciences, Amity University Haryana, Gurugram 122413, India.

Recent advances in woundcare is targeted towards developing active-dressings, where multiple components are combined to provide a suitable environment for rapid healing. The aim of the present research is to study the preparation of biomimic composite wound dressings by the grafting of hydrogel on silk fibroin fabric. The swelling ability of hydrogel grafted silk fibroin fabric was optimized by changing the initiator concentration. Read More

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http://dx.doi.org/10.3390/biom10050710DOI Listing

Functional Relationship between Osteogenesis and Angiogenesis in Tissue Regeneration.

Int J Mol Sci 2020 May 3;21(9). Epub 2020 May 3.

Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy.

Bone tissue renewal can be outlined as a complicated mechanism centered on the interaction between osteogenic and angiogenic events capable of leading to bone formation and tissue renovation. The achievement or debacle of bone regeneration is focused on the primary role of vascularization occurrence; in particular, the turning point is the opportunity to vascularize the bulk scaffolds, in order to deliver enough nutrients, growth factors, minerals and oxygen for tissue restoration. The optimal scaffolds should ensure the development of vascular networks to warrant a positive suitable microenvironment for tissue engineering and renewal. Read More

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http://dx.doi.org/10.3390/ijms21093242DOI Listing

Amelogenin Downregulates Interferon Gamma-Induced Major Histocompatibility Complex Class II Expression Through Suppression of Euchromatin Formation in the Class II Transactivator Promoter IV Region in Macrophages.

Front Immunol 2020 21;11:709. Epub 2020 Apr 21.

Division of Oral Rehabilitation, Department of Periodontology, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.

Enamel matrix derivatives (EMDs)-based periodontal tissue regenerative therapy is known to promote healing with minimal inflammatory response after periodontal surgery, i. e., it promotes wound healing with reduced pain and swelling. Read More

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http://dx.doi.org/10.3389/fimmu.2020.00709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186442PMC

Osteoblasts migration, attachment and human bone marrow-mesenchymal stem cells osteogenic differentiation towards surface engineered and growth factors conjugated poly(lactic acid) microspheres.

J Mater Sci Mater Med 2020 May 4;31(5):45. Epub 2020 May 4.

Biomaterials Niche Group, School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia, 14300, Nibong Tebal, Pulau Pinang, Malaysia.

Recently, surface engineered biomaterials through surface modification are extensively investigated due to its potential to enhance cellular homing and migration which contributes to a successful drug delivery process. This study is focused on osteoblasts response towards surface engineered using a simple sodium hydroxide (NaOH) hydrolysis and growth factors conjugated poly(lactic acid) (PLA) microspheres. In this study, evaluation of the relationship of NaOH concentration with the molecular weight changes and surface morphology of PLA microspheres specifically wall thickness and porosity prior to in vitro studies was investigated. Read More

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http://dx.doi.org/10.1007/s10856-020-06380-yDOI Listing

Immunomodulatory Properties of Chitosan: Impact on Wound Healing and Tissue Repair.

Endocr Metab Immune Disord Drug Targets 2020 May 2. Epub 2020 May 2.

Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak -124001, Haryana. India.

Objective: Chitosan, a natural polymer from shelled crustaceans, have been employed in an array of biomedical applications as it possesses a combination of fascinating attributes such as biocompatibility, biodegradability, non-toxicity and ease of chemical alteration into different active derivatives. The ability of chitosan to be tailored into different shapes has made it a very popular polymer for designing novel drug delivery systems for topical, transdermal and gene delivery. Recently, multifunctional and immunomodulatory properties of chitosan have attracted a lot of attention. Read More

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http://dx.doi.org/10.2174/1871530320666200503054605DOI Listing

IGF-1C domain-modified chitosan hydrogel accelerates cutaneous wound healing by promoting angiogenesis.

Future Med Chem 2020 Apr 30. Epub 2020 Apr 30.

Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, China.

Complete regeneration after skin injury remains a critical clinical challenge. Hydrogels, modified with growth factors or mimicking peptides, have been applied for functional tissue regeneration by increasing the bioactivity of engineered matrices.  We synthesized an injectable biological hydrogel, C domain of IGF-1 (IGF-1C)-modified chitosan (CS-IGF-1C) hydrogel. Read More

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http://dx.doi.org/10.4155/fmc-2020-0071DOI Listing
April 2020
4.000 Impact Factor

Human-like collagen promotes the healing of acetic acid-induced gastric ulcers in rats by regulating NOS and growth factors.

Food Funct 2020 May 29;11(5):4123-4137. Epub 2020 Apr 29.

Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China.

Human-like collagen (HLC), the collagen produced using fermentation technology, has been demonstrated previously to promote wound healing. However, the healing property of HLC in gastric ulcers remains to be verified. In this study, we investigated the healing efficacy and healing mechanisms of HLC on gastric ulcers. Read More

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http://dx.doi.org/10.1039/d0fo00288gDOI Listing

Minicircle-based GCP-2 ex vivo gene therapy enhanced the reepithelialization and angiogenic capacity.

J Tissue Eng Regen Med 2020 Apr 25. Epub 2020 Apr 25.

Institute for Bio-Medical Convergence, Department of Medicine, Catholic Kwandong University College of Medicine, Gangneung, Republic of Korea.

Recently, minicircle (MC)-based cell therapy has been emerging as a novel technology for nonviral genetic modification. In this study, we investigated the characteristics of granulocyte chemotactic protein-2 (GCP-2)-overexpressing fibroblasts (GCP-2/MC) using MC microporation technology, as well as its therapeutic mechanism in wound healing. GCP-2 parent plasmid and MC containing GCP-2 were generated. Read More

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http://dx.doi.org/10.1002/term.3049DOI Listing

Combining the Benefits of Collagen and Negative Pressure Wound Therapy to Heal a Chronic Diabetic Foot Ulcer: A Case Report.

Wounds 2020 Mar;32(3):E11-E13

Foot and Ankle Specialists of Delaware County, Springfield, PA.

Introduction: Diabetes affects 30 million children and adults in the United States, equivalent to 1 out of every 11 people in the country, and results in costs of $327 billion annually. Interventions that can improve healing rates and/or reduce the size of diabetic ulcers may lower the incidence of infection, rate of amputations, and cost of care. This report is on the use of a collagen wound contact layer in conjunction with negative pressure wound therapy (NPWT) to achieve healing in a chronic diabetic foot ulcer (DFU). Read More

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Protocols for decellularization of human amniotic membrane.

Methods Cell Biol 2020 18;157:37-47. Epub 2019 Dec 18.

Cellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran; Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address:

Human amniotic membrane (HAM) has been used as a very promising biological-based product in health centers, especially for skin and cornea wound healing applications. The excellent properties of this membrane make it a potential candidate in treatment of various skin injuries such as bedsores, burn wounds and diabetic ulcers. Such properties are cytobiocompatibility, a structure very similar to normal skin composed of extracellular matrix (ECM) proteins, various growth factors involved in normal wound healing process and antibacterial agents. Read More

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http://dx.doi.org/10.1016/bs.mcb.2019.11.004DOI Listing
December 2019

Neurotrophins BDNF and NT4/5 accelerate dental pulp stem cell migration.

Biomed J 2020 Apr 21. Epub 2020 Apr 21.

Department of Biomedical Sciences, Arthur A. Dugoni School of Dentistry, University of the Pacific, San Francisco, CA, 94103, USA.

Neurotrophic factors play important roles in neuron survival, growth and differentiation. In the present research, the expression of multiple neurotrophins and their effects on cell migration were studied in the dental pulp stem cells (DPSCs). Human DPSCs from five patients were cultured. Read More

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http://dx.doi.org/10.1016/j.bj.2020.03.010DOI Listing

Sustained release of human platelet lysate growth factors by thermosensitive hydroxybutyl chitosan hydrogel promotes skin wound healing in rats.

J Biomed Mater Res A 2020 Apr 23. Epub 2020 Apr 23.

Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

This study evaluated the effect of thermosensitive hydroxybutyl chitosan (HBC) hydrogel loaded with human platelet lysate (hPL) on skin wound healing in rats. hPLs were generated by freeze-thaw method of platelet-rich plasma from healthy donors. Successful grafting of hydroxybutyl group to chitosan molecular chain to obtain HBC hydrogel was confirmed by Fourier-transform infrared spectroscopy. Read More

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http://dx.doi.org/10.1002/jbm.a.36970DOI Listing

Dandelion Polysaccharide Exerts Anti-Angiogenesis Effect on Hepatocellular Carcinoma by Regulating VEGF/HIF-1α Expression.

Front Pharmacol 2020 8;11:460. Epub 2020 Apr 8.

School of Forensic Medicine, Xinxiang Medical University, Xinxiang, China.

Recent studies have revealed that natural plants-derived polysaccharides exhibit potent anti-tumor activity. Our earlier studies suggest that dandelion polysaccharide (DP) inhibits hepatocellular carcinoma (HCC) cell proliferation and . Here, we investigated the effects of DP on the angiogenesis of HCC and the potential molecular mechanisms by which DP regulates angiogenesis. Read More

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http://dx.doi.org/10.3389/fphar.2020.00460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158757PMC

Genetic correction of Werner syndrome gene reveals impaired pro-angiogenic function and HGF insufficiency in mesenchymal stem cells.

Aging Cell 2020 May 22;19(5):e13116. Epub 2020 Apr 22.

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.

WRN mutation causes a premature aging disease called Werner syndrome (WS). However, the mechanism by which WRN loss leads to progeroid features evident with impaired tissue repair and regeneration remains unclear. To determine this mechanism, we performed gene editing in reprogrammed induced pluripotent stem cells (iPSCs) derived from WS fibroblasts. Read More

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http://dx.doi.org/10.1111/acel.13116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253065PMC

Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair.

Polymers (Basel) 2020 Apr 17;12(4). Epub 2020 Apr 17.

Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan.

Vital pulp therapy is an important endodontic treatment. Strategies using growth factors and biological molecules are effective in developing pulp capping materials based on wound healing by the dentin-pulp complex. Our group developed biodegradable viscoelastic polymer materials for tissue-engineered medical devices. Read More

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http://dx.doi.org/10.3390/polym12040937DOI Listing

[Effects of farnesyltransferase silencing on the migration and invasion of tongue squamous cell carcinoma].

Hua Xi Kou Qiang Yi Xue Za Zhi 2020 Apr;38(2):177-184

Medical Center of Stomatology, Qingdao Municipal Hospital, Qingdao 266071, China.

Objective: This study aimed to explore the effects of silencing farnesyltransferase (FTase) on the migration and invasion of tongue squamous cell carcinoma (TSCC) through RNA interference.

Methods: TSCC cells (CAL27 and SCC-4) were cultured in vitro and then transfected with siRNA to silence FTase expression. The tested cells were categorized as follows: experimental group (three RNA interference groups), negative control group, and blank control group. Read More

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http://dx.doi.org/10.7518/hxkq.2020.02.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184273PMC

Leucocyte- and platelet-rich fibrin regulates expression of genes related to early wound healing in human gingival fibroblasts.

J Clin Periodontol 2020 Apr 17. Epub 2020 Apr 17.

Faculty of Dentistry, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.

Background: Leucocyte- and platelet-rich fibrin (L-PRF) is a blood-derived biomaterial rich in leucocytes and platelets embedded in a high-density fibrin network that can be compressed into a membrane and used in surgical applications to stimulate tissue regeneration and wound healing, especially in oral cavity. This study aimed to determine the combined effects of the growth factors and cells present in L-PRF on fibroblasts that directly face the L-PRF membranes placed during surgical procedures.

Methods: The effect of L-PRF from six donors on the expression of 84 key wound healing genes in normal human gingival fibroblasts was tested by RT-qPCR. Read More

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http://dx.doi.org/10.1111/jcpe.13293DOI Listing

Multifunctional Copper-Containing Mesoporous Glass Nanoparticles as Antibacterial and Proangiogenic Agents for Chronic Wounds.

Front Bioeng Biotechnol 2020 31;8:246. Epub 2020 Mar 31.

School of Clinical Dentistry, University of Sheffield, Sheffield, United Kingdom.

The physiological wound healing process involves a cascade of events which could be affected by several factors resulting in chronic, non-healing wounds. The latter represent a great burden especially when bacterial biofilms are formed. The rise in antibiotic resistance amongst infectious microorganisms leads to the need of novel approaches to treat this clinical issue. Read More

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http://dx.doi.org/10.3389/fbioe.2020.00246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136418PMC

Diabetes-induced glucolipotoxicity impairs wound healing ability of adipose-derived stem cells-through the miR-1248/CITED2/HIF-1α pathway.

Aging (Albany NY) 2020 Apr 15;12(8):6947-6965. Epub 2020 Apr 15.

Department of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.

Despite being an attractive cell type for mesenchymal stem cell (MSC) transplantation therapy for wound healing, human adipose-derived stem cells (hADSCs) from diabetes mellitus (DM) patients result in remarkable retention of stem cell activity due to diabetes-induced glucolipotoxicity. We explored the effect of diabetes and medium containing AGEs on the cell activity, phenotype, multipotency, angiogenic potential, and the therapeutic effect of hADSCs. Then, miRNA-1248 was selected by miRNA microarray analysis to further study the core molecular pathways that regulate the wound healing ability of hADSCs. Read More

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http://dx.doi.org/10.18632/aging.103053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202540PMC

Electrical stimulation promotes the proliferation of human keratinocytes, increases the production of keratin 5 and 14, and increases the phosphorylation of ERK1/2 and p38 MAP kinases.

J Tissue Eng Regen Med 2020 Apr 15. Epub 2020 Apr 15.

Département de Chirurgie, Faculté de Médecine, Axe Médecine Régénératrice, Centre de Recherche du CHU de Québec, Université Laval, Quebec, Canada.

Effective wound healing remains a significant clinical challenge in reducing patient morbidity and improving quality of life. Wound healing is a complex process involving the endogenous electrical field. The electrical field can contribute to wound healing by activating keratinocytes to promote reepithelialization. Read More

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http://dx.doi.org/10.1002/term.3040DOI Listing

Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen.

J Clin Med 2020 Apr 12;9(4). Epub 2020 Apr 12.

Department of Oral and Maxillo Facial Sciences, "Sapienza" University of Rome, Via Caserta 6, 00161 Rome, Italy.

Liquid fibrinogen is an injectable platelet concentrate rich in platelets, leukocytes, and fibrinogen obtained by blood centrifugation. The aim of this study was to analyze the release of different growth factors in the liquid fibrinogen at different times and to assess possible correlations between growth factors and cell counts. The concentration of transforming growth factor beta 1 (TGF-β1), platelet-derived growth factor-AB (PDGF-AB), platelet-derived growth factor-BB (PDGF-BB), bone morphogenetic protein 2 (BMP-2), fibroblast growth factor 2 (FGF-2) and vascular endothelial growth factor (VEGF) released by liquid fibrinogen were examined with ELISA at three time points (T0, time of collection; T7, 7 days; T14, 14 days). Read More

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http://dx.doi.org/10.3390/jcm9041099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230328PMC

Dermatopontin, A Novel Adipokine Promoting Adipose Tissue Extracellular Matrix Remodelling and Inflammation in Obesity.

J Clin Med 2020 Apr 9;9(4). Epub 2020 Apr 9.

Metabolic Research Laboratory, Clínica Universidad de Navarra, 31008 Pamplona, Spain.

Compelling evidence suggests that dermatopontin (DPT) regulates collagen and fibronectin fibril formation, the induction of cell adhesion and the prompting of wound healing. We aimed to evaluate the role of DPT on obesity and its associated metabolic alterations as well as its impact in visceral adipose tissue (VAT) inflammation and extracellular matrix (ECM) remodelling. Samples obtained from 54 subjects were used in a case-control study. Read More

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http://dx.doi.org/10.3390/jcm9041069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230369PMC

Case report of an unusual presentation of Staphylococcus aureus induced toxic shock syndrome/hyperimmunoglobulinemia E syndrome.

Medicine (Baltimore) 2020 Apr;99(15):e19746

Department of Microbiology and Immunology University of Iowa, Iowa City, IA.

Rationale: Toxic shock syndrome (TSS) typically is an acute onset multi-organ infection caused by TSS toxin-1 producing Staphylococcus aureus. Herein we describe a highly unusual case report.

Patient Concerns: A male patient self-referred to the University of Minnesota Hospital with a chronic history of S aureus infection with accompanying fever, hypotension, and nonhealing, football-sized lesion on his leg. Read More

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http://dx.doi.org/10.1097/MD.0000000000019746DOI Listing

A human epidermal growth factor-curcumin bandage bioconjugate loaded with mesenchymal stem cell for in vivo diabetic wound healing.

Mater Sci Eng C Mater Biol Appl 2020 Jun 18;111:110751. Epub 2020 Feb 18.

P.G. Department of Biotechnology, Utkal University, Bhubaneswar, India.

Bone-marrow-derived mesenchymal stem cells (MSCs) are of growing interest for the treatment of diabetic wound healing. However, they are often associated with poor proliferation and viability at the wounded site. Here, it is reported the use of human epidermal growth factor -curcumin bandage bioconjugate (EGF-Cur B) loaded with MSCs (MSCs-EGF-Cur B) at the wounded site for diabetic wound healing. Read More

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http://dx.doi.org/10.1016/j.msec.2020.110751DOI Listing