Search Our Scientific Publications & Authors

Pathol Res Pract 2021 May 5;223:153463. Epub 2021 May 5.

Department of Neurosurgery, Beilun District People's Hospital of Ningbo City, Ningbo, Zhejiang, 315800, China. Electronic address:

The presence and clinical significance of IL-17 and IL-17-expressing cells have been studied for several cancers, although their correlation with tumor development remains controversial. Peripheral blood was collected from healthy donors and glioma patients to isolate peripheral blood mononuclear cells (PBMCs). The percentage of IL-17-expressing cells and the production of inflammatory cytokines in PBMCs and tissues were measured. Read More

Cell Signal 2021 May 7:110036. Epub 2021 May 7.

Departamento de Biología Celular y Molecular and Center for Aging and Regeneration (CARE-ChileUC), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 8331150 Santiago, Chile; Fundación Ciencia & Vida, 7780272 Santiago, Chile.

Platelet-derived growth factors (PDGFs) regulate embryonic development, tissue regeneration, and wound healing through their binding to PDGF receptors, PDGFRα and PDGFRβ. However, the role of PDGF signaling in regulating muscle development and regeneration remains elusive, and the cellular and molecular responses of myogenic cells are understudied. Here, we explore the PDGF-PDGFR gene expression changes and their involvement in skeletal muscle myogenesis and myogenic fate. Read More

Wound Repair Regen 2021 May 10. Epub 2021 May 10.

Department of Applied Biology, Council of Scientific & Industrial Research-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad, India.

Skin regeneration has been a challenging clinical problem especially in cases of chronic wounds such as diabetic foot ulcers, and epidermolysis bullosa-related skin blisters. Prolonged non-healing wounds often lead to bacterial infections increasing the severity of wounds. Current treatment strategies for chronic wounds include debridement of wounds along with antibiotics, growth factors, and stem cell transplantation therapies. Read More

Front Pharmacol 2021 23;12:627098. Epub 2021 Apr 23.

Department of Vascular Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

To investigate the clinical efficacy of epidermal growth factor combined with nano silver dressing in the treatment of diabetic foot wounds. A total of 160 patients with diabetic foot ulcers admitted to the Second Affiliated Hospital of Nanchang University from 2015-06 to 2018-06 were selected to participate in the experiment. A randomized table method was used to randomly divide 160 patients into 4 groups: 40 in the epidermal growth factor group, 40 in the nano-silver dressing group, 40 in the combined group, and 40 in the saline control group (normal saline). Read More

Ann Palliat Med 2021 Apr;10(4):4882-4888

Department of Burns and Plastic, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.

Objective: This article aims to pay attention to the latest research on the expression, activation and function of hypoxia-inducible factor-2α (HIF-2α) under hypoxia and non-hypoxia conditions, and summarizes the current knowledge about the interaction between hypoxia-inducible factor-2 and angiogenesis, hoping to understand its actions in physiology and disease, with the goal of providing a new strategy for the diagnosis and treatment of wounds.

Background: Wound healing is a complex and continuous process, involving coagulation, inflammation, angiogenesis, new tissue formation and extracellular matrix remodeling. Of these, angiogenesis is an essential step. Read More

PLoS One 2021 6;16(5):e0247098. Epub 2021 May 6.

Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Introduction: Pressure ulcer (PU) is known as the third most costly disorder usually caused by prolonged pressure and stagnation in various parts of the body. Although several therapeutic approaches are employing, obstacles in appropriate healing for skin lesions still exist which necessitates new practical alternative or adjunctive treatments. Low level laser therapy (LLLT) as one of the mentioned new strategies have gained attention. Read More

Int Wound J 2021 May 5. Epub 2021 May 5.

Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Monterrey, Nuevo León, Mexico.

Wound healing is a complex process of communication between growth factors, reactive species of oxygen, cells, signalling pathways, and cytokines in the extracellular matrix, in which growth factors are the key regulators. In humans, the main regulators of the cellular responses in wound healing are five growth factors, namely EGF, bFGF, VEGF, and TGF-β1. On the other hand, antioxidants such as astaxanthin, beta-carotene, epigallocatechin gallate, delphinidin, and curcumin have been demonstrated to stimulate cell proliferation, migration and angiogenesis, and control inflammation, to suggest a practical approach to design new strategies to treat non-healing cutaneous conditions. Read More

Pharmaceutics 2021 Apr 29;13(5). Epub 2021 Apr 29.

National Research Council (CNR), Campus Ecotekne, Institute of Clinical Physiology (IFC), University of Salento, Via per Monteroni, 73100 Lecce, Italy.

Blood-derived concentrated growth factors (CGFs) represent a novel autologous biomaterial with promising applications in regenerative medicine. Angiogenesis is a key factor in tissue regeneration, but the role played by CGFs in vessel formation is not clear. The purpose of this study was to characterize the angiogenic properties of CGFs by evaluating the effects of its soluble factors and cellular components on the neovascularization in an in vitro model of angiogenesis. Read More

Ann Clin Lab Sci 2021 Mar;51(2):182-189

Department of Obstetrics, Jiaxing Municipal Maternal and Child Health Care Hospital

Objective: Fetal growth restriction (FGR) refers to impaired and insufficient intrauterine growth potential caused by a variety of adverse factors and is a serious perinatal complication that leads to fetal or neonatal mortality and morbidity. FGR has numerous causes, and its pathogenesis has not been fully understood. Recently, increasing numbers of researchers have begun to focus on the placenta, the only link between the fetus and the mother. Read More

Tissue Cell 2021 Apr 21;72:101548. Epub 2021 Apr 21.

Department of Dermatology, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China.

Dermal fibroblasts are a promising candidate for cellular-based therapies for thermal wound healing because of their capacity of producing extracellular matrix (ECM), promoting wound contraction and the synthesis of type I collagen, and secreting growth factors. miRNAs (MicroRNAs) might mediate the role of TGF-β1(Transforming Growth Factor-beta 1), one of the major profibrotic cytokines, in improving thermal injury repair. In the present study, we observed the abnormal downregulation of TGF-β1 following thermal injury in the burnt dermis (in vivo) and heat-stimulated human dermal fibroblasts (in vitro). Read More

J Drugs Dermatol 2021 May;20(5):538-545

Background: Applied topically, growth factors, cytokines, and other components in bovine colostrum are known to affect collagen biosynthesis, thus offering promise as a therapeutic modality in wound healing, delay in skin aging, and skin rejuvenation.

Objective: To demonstrate the protective effect that liposomal bovine colostrum exerts on skin aging using telomere length as an aging biomarker.

Methods: Human fibroblasts were cultured for 8 weeks with colostrum at three concentrations (0. Read More

Burns 2021 Apr 14. Epub 2021 Apr 14.

Discipline of Plastic Surgery, Surgery Department, (UNIFESP/EPM), Brazil.

Introduction: A burn is a trauma that breaks the skin barrier, causing local and systemic responses. Treatment is complex, multiprofessional and expensive. In addition to surgical treatment, topical dressings can be used to keep the wound moist, reduce the risk of infection and stimulate healing. Read More

Toxicon 2021 Apr 27. Epub 2021 Apr 27.

Department of Orthopaedics, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China. Electronic address:

Melittin is a major active peptide component of bee venom that has been demonstrated to show anti-tumor effects. Osteosarcoma is a type of bone tumor with a high degree of malignancy, and metastasis is the main challenge of osteosarcoma therapy. This study aimed to investigate the role of melittin in the lung metastasis of osteosarcoma. Read More

Bioengineering (Basel) 2021 Apr 27;8(5). Epub 2021 Apr 27.

Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, Greece.

This article provides additional knowledge for cord blood platelet gel (CBPG) production. Recently, it has been shown that CBPG exerts beneficial properties in wound healing applications. CBPG is produced after a two-step centrifugation process, following the addition of calcium gluconate. Read More

Nanomaterials (Basel) 2021 Apr 13;11(4). Epub 2021 Apr 13.

Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, 1083, 142 20 Prague, Czech Republic.

Platelet lysate (PL) provides a natural source of growth factors and other bioactive molecules, and the local controlled release of these bioactive PL components is capable of improving the healing of chronic wounds. Therefore, we prepared composite nanofibrous meshes via the needleless electrospinning technique using poly(vinyl alcohol) (PVA) with a high molecular weight and with a high degree of hydrolysis with the incorporated PL (10% ). The morphology, wettability and protein release from the nanofibers was then assessed from the resulting composite PVA-PL nanomats. Read More

Animals (Basel) 2021 Apr 23;11(5). Epub 2021 Apr 23.

Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università 16, Legnaro, 35020 Padova, Italy.

Skin wound healing is a complex and dynamic process that aims to restore lesioned tissues. Collagen-based skin substitutes are a promising treatment to promote wound healing by mimicking the native skin structure. Recently, collagen from marine organisms has gained interest as a source for producing biomaterials for skin regenerative strategies. Read More

Molecules 2021 Apr 23;26(9). Epub 2021 Apr 23.

Ostrowieckie Centrum Medyczne Civil Partnership Anna Olech-Cudzik, Krzysztof Cudzik Iłżecka 31A, 27-400 Ostrowiec Świętokrzyski, Poland.

, one of the most popular medicinal plants, is the source of a bioactive essential oil characterized by a wide spectrum of biological activity, e.g., antiseptic, analgesic, and anticancer effects. Read More

Int J Mol Sci 2021 Apr 14;22(8). Epub 2021 Apr 14.

Department of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland.

The aim of the present study was to investigate the influence of a novel volume-stable collagen matrix (vCM) on early wound healing events including cellular migration and adhesion, protein adsorption and release, and the dynamics of the hemostatic system. For this purpose, we utilized transwell migration and crystal violet adhesion assays, ELISAs for quantification of adsorbed and released from the matrix growth factors, and qRT-PCR for quantification of gene expression in cells grown on the matrix. Our results demonstrated that primary human oral fibroblasts, periodontal ligament, and endothelial cells exhibited increased migration toward vCM compared to control cells that migrated in the absence of the matrix. Read More

Radiat Res 2021 Apr 29. Epub 2021 Apr 29.

Radiation Combined Injury Program, Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20889.

Radiation combined injury (RCI, radiation exposure coupled with other forms of injury, such as burn, wound, hemorrhage, blast, trauma and/or sepsis) comprises approximately 65% of injuries from a nuclear explosion, and greatly increases the risk of morbidity and mortality when compared to that of radiation injury alone. To date, no U.S. Read More

Biofilm 2021 Dec 3;3:100047. Epub 2021 Apr 3.

Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, India.

Bacterial biofilms are a major cause of delayed wound healing. Consequently, the study of wound biofilms, particularly in host-relevant conditions, has gained importance. Most studies employ refined laboratory media to study biofilms, representing conditions that are not relevant to the infection state. Read More

Front Bioeng Biotechnol 2021 13;9:641505. Epub 2021 Apr 13.

Wenzhou Medical University, Chashan University Park, Wenzhou, China.

In this study, the optimum human gene encoding haFGF was cloned in pET3c and transferred to BL21(DE3) plysS. To enhance the yield of fermentation and the expression level of the target protein, the fermentation parameters, including temperature, pH, dissolved oxygen, glucose concentration, ammonium chloride concentration, induction time, and inducer (IPTG) concentration, were optimized. The optimized fermentation parameters were used in large-scale fermentation (30 L). Read More

Cancer Chemother Pharmacol 2021 Apr 27. Epub 2021 Apr 27.

Department of Medicine I, Clinical Division of Oncology, Comprehensive Cancer Center of the Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.

Purpose: As critical parameter after extravasation of cytotoxic vesicants, anthracyclines were determined in removed tissue from patients requiring surgical intervention due to tissue necrosis. We monitored their distribution within the affected lesion to establish a possible dose-toxicity relation.

Methods: From six patients scheduled for surgery, removed tissue flaps were systematically analysed by HPLC (epirubicin: 5 subjects; doxorubicin: 1 subject). Read More

Ann Anat 2021 Apr 24;237:151745. Epub 2021 Apr 24.

Institute of Functional and Clinical Anatomy, Friedrich Alexander University Erlangen-Nürnberg, Erlangen, Germany; Sechenov University, Department of Operative Surgery and Topographic Anatomy, Moscow, Russia.

Background: Wound healing disorders characterised by impaired or delayed reepithelialisation are a serious medical problem. In the present study, we show that gold-based blood serum therapy is a suitable therapeutic approach and shows a supportive effect in wound closure of human corneal epithelial cells (HCE) in primary in vitro experiments.

Methods: For this purpose, blood from healthy individuals was incubated without (S. Read More

Biomacromolecules 2021 May 26;22(5):1885-1900. Epub 2021 Apr 26.

Centre for Biomedical Engineering, Indian Institute of Technology, Delhi, New Delhi 110016, India.

Diabetes mellitus (DM)-associated impairments in wound healing include prolonged inflammation, the overexpression of matrix metalloproteases (MMPs), and low levels of growth factors at the wound site. To this end, a layer-by-layer scaffold (S) made of cross-linked silk fibroin and hyaluronic acid is developed to deliver chlorhexidine, an antimicrobial agent and an MMP-9 inhibitor, along with the PDGF-BB protein. S exhibited highly porous morphology. Read More

Vopr Kurortol Fizioter Lech Fiz Kult 2021 ;98(2):45-54

Skin and venereal clinic N4, St.-Petersburg, Russia.

The scope of physical methods for correcting the pathological skin scars is rapidly growing but the results of their use are often unsatisfactory. An inadequate approach to therapy without taking into account the assessment of their effectiveness leads to relapses, increased growth of scar tissue or the absence of a clinically significant effect which requires strict scientific evidence in the course of benign scientific studies.

Objective: Scientometric analysis of evidence-based studies on the use of therapeutic physical factors in the correction of post-acne scars. Read More

Front Cell Dev Biol 2021 7;9:654084. Epub 2021 Apr 7.

Department of Pediatric Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Tendon repair is a medical challenge. Our present study investigated the effectiveness of acellular therapy consisting of conditioned medium (CM) of tendon stem cells (TSCs) induced with hepatocyte growth factor (HGF) in promoting the healing of injured Achilles tendon in a rat model. Proteomic analysis of soluble substances in the CM was performed using an array chip, and bioinformatic analysis was carried out to evaluate interactions among the factors. Read More

Ann Med Surg (Lond) 2021 Apr 26;64:102254. Epub 2021 Mar 26.

Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.

Background: The healing process of burns includes coagulation, inflammation, and remodeling. Vascular endothelial growth factor (VEGF) is involved throughout this healing process. Stem cells from the platelet-rich plasma (PRP) with stromal vascular fraction (SVF) can increase concentrations of growth factors, including VEGF. Read More

J Surg Res 2021 Apr 22;265:100-113. Epub 2021 Apr 22.

Department of Pathophysiology, College of Basic Medical Science, China Medical University, Shenyang, China. Electronic address:

Background: Stem cell-based regenerative therapy is a novel approach to severe damaged skin. Perinatal tissues such as placenta are considered as promising alternatives. The present study aimed to investigate the effect of insulin-like growth factor-1 (IGF-1)-expressing placenta-derived mesenchymal stem cells (hPMSCs) on healing of burn wounds. Read More

Exp Eye Res 2021 Apr 22;207:108594. Epub 2021 Apr 22.

The Cole Eye Institute, The Cleveland Clinic, Cleveland, OH, USA. Electronic address:

The TGF beta-1, -2 and -3 isoforms are transcribed from different genes but bind to the same receptors and signal through the same canonical and non-canonical signal transduction pathways. There are numerous regulatory mechanisms controlling the action of each isoform that include the organ-specific cells producing latent TGF beta growth factors, multiple effectors that activate the isoforms, ECM-associated SLRPs and basement membrane components that modulate the activity and localization of the isoforms, other interactive cytokine-growth factor receptor systems, such as PDGF and CTGF, TGF beta receptor expression on target cells, including myofibroblast precursors, receptor binding competition, positive and negative signal transduction effectors, and transcription and translational regulatory mechanisms. While there has long been the view that TGF beta-1and TGF beta-2 are pro-fibrotic, while TGF beta-3 is anti-fibrotic, this review suggests that view is too simplistic, at least in adult tissues, since TGF beta-3 shares far more similarities in its modulation of fibrotic gene expression with TGF beta-1 and TGF beta-2, than it does differences, and often the differences are subtle. Read More

Pril (Makedon Akad Nauk Umet Odd Med Nauki) 2021 Apr 23;42(1):127-139. Epub 2021 Apr 23.

University Clinic for Plastic and Reconstructive Surgery, Skopje, RN Macedonia.

Wound healing is a dynamic and physiological process for restoring the normal architecture and functionality of damaged tissue. Platelet-rich plasma (PRP) is an autologous whole blood product that contains a large number of platelets in a small volume of plasma with complete set of coagulation factors, which are in physiological concentrations. PRP has haemostatic, adhesive properties and acts supraphysiologically in the process of wound healing and osteogenesis. Read More