Ribosomal DNA status inferred from DNA cloud assays and mass spectrometry identification of agarose-squeezed proteins interacting with chromatin (ASPIC-MS).
- Kamil Krol,
- Justyna Jendrysek,
- Janusz Debski,
- Marek Skoneczny,
- Anna Kurlandzka,
- Joanna Kaminska,
- Michal Dadlez,
- Adrianna Skoneczna
Oncotarget 2017 Feb 15. Epub 2017 Feb 15.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Laboratory of Mutagenesis and DNA Repair, Warsaw, 02-106, Poland.
Ribosomal RNA-encoding genes (rDNA) are the most abundant genes in eukaryotic genomes. To meet the high demand for rRNA, rDNA genes are present in multiple tandem repeats clustered on a single or several chromosomes and are vastly transcribed. To facilitate intensive transcription and prevent rDNA destabilization, the rDNA-encoding portion of the chromosome is confined in the nucleolus. Read More