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    23551 results match your criteria Viral Hemorrhagic Fevers

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    Interactome analysis of the lymphocytic choriomeningitis virus nucleoprotein in infected cells reveals ATPase Na+/K+ transporting subunit Alpha 1 and prohibitin as host-cell factors involved in the life cycle of mammarenaviruses.
    PLoS Pathog 2018 Feb 20;14(2):e1006892. Epub 2018 Feb 20.
    Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, California, United States of America.
    Several mammalian arenaviruses (mammarenaviruses) cause hemorrhagic fevers in humans and pose serious public health concerns in their endemic regions. Additionally, mounting evidence indicates that the worldwide-distributed, prototypic mammarenavirus, lymphocytic choriomeningitis virus (LCMV), is a neglected human pathogen of clinical significance. Concerns about human-pathogenic mammarenaviruses are exacerbated by of the lack of licensed vaccines, and current anti-mammarenavirus therapy is limited to off-label use of ribavirin that is only partially effective. Read More

    Mammarenaviruses deleted from their Z gene are replicative and produce an infectious progeny in BHK-21 cells.
    Virology 2018 Feb 13;518:34-44. Epub 2018 Feb 13.
    Unité de virologie, Département de Biologie des Agents Transmissibles, Institut de Recherche Biomédicale des Armées, 1 place général Valérie André, BP 73 91 223 Brétigny-sur-Orge cedex, France. Electronic address:
    Mammarenaviruses bud out of infected cells via the recruitment of the endosomal sorting complex required for transport through late domain motifs localized into their Z protein. Here, we demonstrated that mammarenaviruses lacking this protein can be rescued and are replicative, despite a 3-log reduction in virion production, in BHK-21 cells, but not in five other cell lines. Mutations of putative late domain motifs identified into the viral nucleoprotein resulted in the almost complete abolition of infectious virion production by Z-deleted mammarenaviruses. Read More

    Sending the Right Message: Wild Game and the West Africa Ebola Outbreak.
    Health Secur 2018 Jan/Feb;16(1):48-57
    The unprecedented scale of the Ebola epidemic in West Africa in 2014-15 caught the world by surprise. Zaire Ebolavirus had not previously been documented in Guinea, Sierra Leone, or Liberia. However, since this strain of filovirus was first identified in 1976, scientists have been studying the disease and its origins. Read More

    Outbreak of Seoul Virus Among Rats and Rat Owners - United States and Canada, 2017.
    MMWR Morb Mortal Wkly Rep 2018 Feb 2;67(4):131-134. Epub 2018 Feb 2.
    In December 2016, the Wisconsin Department of Health Services (WDHS) notified CDC of a patient hospitalized with fever, leukopenia, elevated transaminases, and proteinuria. The patient owned and operated an in-home rattery, or rat-breeding facility, with approximately 100 Norway rats, primarily bred as pets. A family member developed similar symptoms 4 weeks later, but was not hospitalized. Read More

    Identifying protein biomarkers in predicting disease severity of dengue virus infection using immune-related protein microarray.
    Medicine (Baltimore) 2018 Feb;97(5):e9713
    Faculty of Medicine, MAHSA University, Bandar Saujana Putra.
    Dengue virus is one of the most widespread flaviviruses that re-emerged throughout recent decades. The progression from mild dengue to severe dengue (SD) with the complications such as vascular leakage and hemorrhage increases the fatality rate of dengue. The pathophysiology of SD is not entirely clear. Read More

    Alphavirus Replicon DNA Vectors Expressing Ebola GP and VP40 Antigens Induce Humoral and Cellular Immune Responses in Mice.
    Front Microbiol 2017 10;8:2662. Epub 2018 Jan 10.
    Department of Human Parasitology, Wenzhou Medical University, Wenzhou, China.
    Ebola virus (EBOV) causes severe hemorrhagic fevers in humans, and no approved therapeutics or vaccine is currently available. Glycoprotein (GP) is the major protective antigen of EBOV, and can generate virus-like particles (VLPs) by co-expression with matrix protein (VP40). In this study, we constructed a recombinant Alphavirus Semliki Forest virus (SFV) replicon vector DREP to express EBOV GP and matrix viral protein (VP40). Read More

    Chikungunya Outbreak in Kedougou, Southeastern Senegal in 2009-2010.
    Open Forum Infect Dis 2018 Jan 2;5(1):ofx259. Epub 2017 Dec 2.
    Institut Pasteur Dakar, Arbovirus and Viral Hemorrhagic Fevers Unit, Senegal.
    Background: In Senegal, Chikungunya virus (CHIKV), which is an emerging mosquito-borne alphavirus, circulates in a sylvatic and urban/domestic cycle and has caused sporadic human cases and epidemics since 1960s. However, the real impact of the CHIKV sylvatic cycle in humans and mechanisms underlying its emergence still remains unknown.

    Methodology: One thousand four hundred nine suspect cases of CHIKV infection, recruited from 5 health facilities located in Kedougou region, south-eastern Senegal, between May 2009 to March 2010, together with 866 serum samples collected from schoolchildren from 4 elementary schools in May and November 2009 from Kedougou were screened for anti-CHIKV immunoglobulin (Ig)M antibodies and, when appropriate, for viral nucleic acid by real-time polymerase chain reaction (rPCR) and virus isolation. Read More

    A Gap Analysis Survey of US Aircraft Rescue and Fire Fighting (ARFF) Members to Determine Highly Infectious Disease Training and Education Needs.
    Disaster Med Public Health Prep 2018 Jan 21:1-5. Epub 2018 Jan 21.
    7Department of Applied Health Science,Indiana University School of Public Health,Bloomington,Indiana.
    Objective: Despite lessons learned from the recent Ebola epidemic, attempts to survey and determine non-health care worker, industry-specific needs to address highly infectious diseases have been minimal. The aircraft rescue and fire fighting (ARFF) industry is often overlooked in highly infectious disease training and education, even though it is critical to their field due to elevated occupational exposure risk during their operations.

    Methods: A 44-question gap analysis survey was distributed to the ARFF Working Group to determine where highly infectious education and training can be improved. Read More

    An experience in the clinical use of specific immunoglobulin from horse blood serum for prophylaxis of Ebola haemorrhagic fever.
    Vopr Virusol 2017;62(1):25-9
    The aim of this work was to estimate the efficacy and safety of single intramuscular introduction of specific heterologous immunoglobulin as prophylactic drug against Ebola hemorrhagic fever. Materials and methods. The specific heterologous immunoglobulin was introduced as a special prophylactic drug to 28 patients in epidemic situations, after skin hurt with infectious materials or contact with infectious blood. Read More

    Establishing Ebola Virus Disease (EVD) diagnostics using GeneXpert technology at a mobile laboratory in Liberia: Impact on outbreak response, case management and laboratory systems strengthening.
    PLoS Negl Trop Dis 2018 01 5;12(1):e0006135. Epub 2018 Jan 5.
    Department of Public Health Emergencies, Ministry of Health, Monrovia, Montserrado, Liberia.
    The 2014-16 Ebola Virus Disease (EVD) outbreak in West Africa highlighted the necessity for readily available, accurate and rapid diagnostics. The magnitude of the outbreak and the re-emergence of clusters of EVD cases following the declaration of interrupted transmission in Liberia, reinforced the need for sustained diagnostics to support surveillance and emergency preparedness. We describe implementation of the Xpert Ebola Assay, a rapid molecular diagnostic test run on the GeneXpert platform, at a mobile laboratory in Liberia and the subsequent impact on EVD outbreak response, case management and laboratory system strengthening. Read More

    Ensemble method for dengue prediction.
    PLoS One 2018 3;13(1):e0189988. Epub 2018 Jan 3.
    Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland, United States of America.
    Background: In the 2015 NOAA Dengue Challenge, participants made three dengue target predictions for two locations (Iquitos, Peru, and San Juan, Puerto Rico) during four dengue seasons: 1) peak height (i.e., maximum weekly number of cases during a transmission season; 2) peak week (i. Read More

    Improving mapping for Ebola response through mobilising a local community with self-owned smartphones: Tonkolili District, Sierra Leone, January 2015.
    PLoS One 2018 3;13(1):e0189959. Epub 2018 Jan 3.
    Manson Unit, Médecins Sans Frontières, London, United Kingdom.
    Background: During the 2014-16 Ebola virus disease (EVD) outbreak, the Magburaka Ebola Management Centre (EMC) operated by Médecins Sans Frontières (MSF) in Tonkolili District, Sierra Leone, identified that available district maps lacked up-to-date village information to facilitate timely implementation of EVD control strategies. In January 2015, we undertook a survey in chiefdoms within the MSF EMC catchment area to collect mapping and village data. We explore the feasibility and cost to mobilise a local community for this survey, describe validation against existing mapping sources and use of the data to prioritise areas for interventions, and lessons learned. Read More

    Epidemiological, clinical and climatic characteristics of dengue fever in Kaohsiung City, Taiwan with implication for prevention and control.
    PLoS One 2018 2;13(1):e0190637. Epub 2018 Jan 2.
    Kaohsiung Municipal Kai-Syuan Psychiatric Hospital, Kaohsiung, Taiwan.
    Background: The early identification of dengue infection is essential for timely and effective quarantine and vector control measures for preventing outbreaks of the disease. Kaohsiung City is responsible for most of the dengue cases in Taiwan. Thus, this study aims to identify major factors involved in the prevalence of dengue fever by analyzing the epidemiological and clinical characteristics, and to establish associations between weather parameters and dengue occurrence in this City. Read More

    Insecticide treated curtains and residual insecticide treatment to control Aedes aegypti: An acceptability study in Santiago de Cuba.
    PLoS Negl Trop Dis 2018 01 2;12(1):e0006115. Epub 2018 Jan 2.
    Department of Public Health, Institute of Tropical Medicine, Antwerp, Belgium.
    Background: Within the context of a field trial conducted by the Cuban vector control program (AaCP), we assessed acceptability of insecticide-treated curtains (ITCs) and residual insecticide treatment (RIT) with deltamethrin by the community. We also assessed the potential influence of interviewees' risk perceptions for getting dengue and disease severity.

    Methodology/principal Findings: We embedded a qualitative study using in-depth interviews in a cluster randomized trial (CRT) testing the effectiveness of ITCs and RIT in Santiago de Cuba. Read More

    Unexpected high circulation of Plasmodium vivax in asymptomatic children from Kédougou, southeastern Senegal.
    Malar J 2017 Dec 29;16(1):497. Epub 2017 Dec 29.
    Immunology Unit, Institut Pasteur de Dakar, Dakar, Senegal.
    Background: Malaria in Senegal is due essentially to infections by Plasmodium falciparum and, to a lesser extent to Plasmodium malariae and Plasmodium ovale. By the use of molecular methods, detection of Plasmodium vivax has been recently reported in the region of Kedougou, raising the question of appraisal of its potential prevalence in this setting.

    Methods: A retrospective serological study was carried out using 188 samples taken from 2010 to 2011 in a longitudinal school survey during which 48 asymptomatic children (9-11 years) were recruited. Read More

    Transmission of Rift Valley fever virus from European-breed lambs to Culex pipiens mosquitoes.
    PLoS Negl Trop Dis 2017 12 27;11(12):e0006145. Epub 2017 Dec 27.
    Department of Virology, Wageningen Bioveterinary Research, Lelystad, the Netherlands.
    Background: Rift Valley fever virus (RVFV) is a mosquito-borne bunyavirus of the genus Phlebovirus that is highly pathogenic to ruminants and humans. The disease is currently confined to Africa and the Arabian Peninsula, but globalization and climate change may facilitate introductions of the virus into currently unaffected areas via infected animals or mosquitoes. The consequences of such an introduction will depend on environmental factors, the availability of susceptible ruminants and the capacity of local mosquitoes to transmit the virus. Read More

    Mutational analysis of Rift Valley fever phlebovirus nucleocapsid protein indicates novel conserved, functional amino acids.
    PLoS Negl Trop Dis 2017 12 21;11(12):e0006155. Epub 2017 Dec 21.
    MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
    Rift Valley fever phlebovirus (RVFV; Phenuiviridae, Phlebovirus) is an important mosquito-borne pathogen of both humans and ruminants. The RVFV genome is composed of tripartite, single stranded, negative or ambisense RNAs. The small (S) segment encodes both the nucleocapsid protein (N) and the non-structural protein (NSs). Read More

    Individual co-variation between viral RNA load and gene expression reveals novel host factors during early dengue virus infection of the Aedes aegypti midgut.
    PLoS Negl Trop Dis 2017 12 19;11(12):e0006152. Epub 2017 Dec 19.
    Insect-Virus Interactions Group, Department of Genomes and Genetics, Institut Pasteur, Paris, France.
    Dengue virus (DENV) causes more human infections than any other mosquito-borne virus. The current lack of antiviral strategies has prompted genome-wide screens for host genes that are required for DENV infectivity. Earlier transcriptomic studies that identified DENV host factors in the primary vector Aedes aegypti used inbred laboratory colonies and/or pools of mosquitoes that erase individual variation. Read More

    Long-lived CD8+ T cell responses following Crimean-Congo haemorrhagic fever virus infection.
    PLoS Negl Trop Dis 2017 12 19;11(12):e0006149. Epub 2017 Dec 19.
    Division of Virology, National Health Laboratory Service/University of the Free State, Bloemfontein, South Africa.
    Crimean-Congo haemorrhagic fever virus (CCHFV) is a member of the Orthonairovirus genus of the Nairoviridae family and is associated with haemorrhagic fever in humans. Although T lymphocyte responses are known to play a role in protection from and clearance of viral infections, specific T cell epitopes have yet to be identified for CCHFV following infection. A panel of overlapping peptides covering the CCHFV nucleoprotein and the structural glycoproteins, GN and GC, were screened by ELISpot assay to detect interferon gamma (IFN-γ) production in vitro by peripheral blood mononuclear cells from eleven survivors with previous laboratory confirmed CCHFV infection. Read More

    Enhancing the antiviral potency of ER α-glucosidase inhibitor IHVR-19029 against hemorrhagic fever viruses in vitro and in vivo.
    Antiviral Res 2018 02 15;150:112-122. Epub 2017 Dec 15.
    Baruch S. Blumberg Institute, Hepatitis B Foundation, Doylestown, PA, USA. Electronic address:
    Targeting host functions essential for viral replication has been considered as a broad spectrum and resistance-refractory antiviral approach. However, only a few host functions have, thus far, been validated as broad-spectrum antiviral targets in vivo. ER α-glucosidases I and II have been demonstrated to be essential for the morphogenesis of many enveloped viruses, including members from four families of viruses causing hemorrhagic fever. Read More

    Aedes aegypti breeding site in an underground rainwater reservoir: a warning.
    Rev Saude Publica 2017 Dec 11;51:122. Epub 2017 Dec 11.
    Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiología. São Paulo, SP, Brasil.
    We describe the discovery of Aedes aegypti underground breeding site in the Pinheiros neighborhood of São Paulo, SP, during an entomological survey program performed in 2016. Even with intense surveillance and vector control, large numbers of mosquitoes were present in this area. A detailed investigation allowed for the detection of Ae. Read More

    Translating Predictions of Zoonotic Viruses for Policymakers.
    Ecohealth 2017 Dec 11. Epub 2017 Dec 11.
    University of California, Los Angeles, CA, USA.
    Recent outbreaks of Ebola virus disease and Zika virus disease highlight the need for disseminating accurate predictions of emerging zoonotic viruses to national governments for disease surveillance and response. Although there are published maps for many emerging zoonotic viruses, it is unknown if there is agreement among different models or if they are concordant with national expert opinion. Therefore, we reviewed existing predictions for five high priority emerging zoonotic viruses with national experts in Cameroon to investigate these issues and determine how to make predictions more useful for national policymakers. Read More

    Estimating Vector-borne Viral Infections in the Urban Setting of the 2020 Tokyo Olympics, Japan, Using Mathematical Modeling.
    Tokai J Exp Clin Med 2017 Dec 20;42(4):160-164. Epub 2017 Dec 20.
    Basic Clinical Science and Public Health, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
    The first domestic outbreak of dengue fever in Japan since 1945 was reported in Tokyo in 2014. Meanwhile, daily mean summer temperatures are expected to continue to rise world-wide. Such conditions are expected to increase the risk of an arbovirus outbreak at the 2020 Tokyo Olympic Games. Read More

    US State Public Health Departments Special Pathogen Planning.
    J Public Health Manag Pract 2017 Dec 7. Epub 2017 Dec 7.
    Departments of Environmental, Agricultural & Occupational Health (Ms Herstein and Dr Lowe) and Internal Medicine, Division of Infectious Diseases (Dr Hewlett) and College of Medicine (Ms Jelden), University of Nebraska Medical Center, Omaha, Nebraska; Department of Emergency Medicine, Division of Emergency Preparedness, Massachusetts General Hospital, Boston, Massachusetts (Dr Biddinger); Department of Emergency Medicine, Harvard Medical School, Boston, Massachusetts (Dr Biddinger); Department of Environmental and Occupational Health, Indiana University School of Public Health, Bloomington, Indiana (Dr Gibbs and Ms Le); and Nebraska Biocontainment Unit, Omaha, Nebraska (Drs Hewlett and Lowe).
    Context: US state public health departments played key roles in planning for and responding to confirmed and suspected cases of Ebola virus disease (EVD) during the 2014-2016 outbreak, including designating select hospitals as high-level isolation units (HLIUs) for EVD treatment in conjunction with the Centers for Disease Control and Prevention.

    Objective: To identify existing guidelines and perspectives of state health departments pertaining to the management and transport of patients with EVD and other highly hazardous communicable diseases (HHCDs).

    Design: An electronic 8-question survey with subquestions was administered as a fillable PDF. Read More

    Dengue hemorrhagic fever complicated with acute liver failure: a case report.
    J Med Case Rep 2017 Dec 8;11(1):341. Epub 2017 Dec 8.
    Department of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.
    Background: Dengue is a common arboviral infection with a clinically diverse spectrum of presentations. Although hepatic dysfunction is commonly identified in patients will dengue illness, acute liver failure is rare. The etiopathogenesis of hepatic dysfunction is multifactorial and related to direct viral invasion of hepatocytes, immunological factors and hypoxia particularly in cases of shock in dengue hemorrhagic fever. Read More

    Symptoms associated with adverse dengue fever prognoses at the time of reporting in the 2015 dengue outbreak in Taiwan.
    PLoS Negl Trop Dis 2017 Dec 6;11(12):e0006091. Epub 2017 Dec 6.
    Institute of Allied Health Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
    Background: Tainan experienced the most severe dengue epidemic in Taiwan in 2015. This study investigates the association between the signs and symptoms at the time of reporting with the adverse dengue prognoses.

    Methods: A descriptive study was conducted using secondary data from the Dengue Disease Reporting System in Tainan, Taiwan, between January 1 and December 31, 2015. Read More

    Metagenomic analysis reveals Hepatitis A virus in suspected yellow fever cases in Brazil.
    Mem Inst Oswaldo Cruz 2018 Jan;113(1):66-67
    Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Genética Molecular de Microrganismos, Rio de Janeiro, RJ, Brasil.
    Using a metagenomic approach, we identified hepatitis A virus among cases of acute febrile illnesses that occurred in 2008-2012 in Brazil suspected as yellow fever. These findings reinforce the challenge facing routine clinical diagnosis in complex epidemiological scenarios. Read More

    Analysing increasing trends of Guillain-Barré Syndrome (GBS) and dengue cases in Hong Kong using meteorological data.
    PLoS One 2017 4;12(12):e0187830. Epub 2017 Dec 4.
    Department of Applied Mathematics, Hong Kong Polytechnic University, Hong Kong SAR, China.
    Background: Guillain-Barré Syndrome (GBS) is a severe paralytic neuropathy associated with virus infections such as Zika virus and Chikungunya virus. There were also case reports of dengue fever preceding GBS. With the aim to understand the mechanisms of GBS and dengue outbreaks, this ecological study investigates the relationships between GBS, dengue, meteorological factors in Hong Kong and global climatic factors from January 2000 to June 2016. Read More

    Mountains, Melting Pot, and Microcosm: Health Care Delay and Dengue/Zika Interplay on Hawaii Island.
    Creat Nurs 2016 Nov;22(4):233-242
    Human history in the Hawaiian Islands offers a sobering study in the population dynamics of infectious disease. The indigenous population numbering an estimated half million people prior to Western contact in 1778 was reduced to less than 24,000 by 1920. Much of the decline occurred in the earliest decades after contact with Western diseases including measles, chicken pox, polio, tuberculosis, and venereal disease. Read More

    Operational evaluation of rapid diagnostic testing for Ebola Virus Disease in Guinean laboratories.
    PLoS One 2017 30;12(11):e0188047. Epub 2017 Nov 30.
    Epidemiology, Informatics, Surveillance and Laboratory Branch, Department of Global Health Protection, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
    Background: Rapid Diagnostic Tests (RDTs) for Ebola Virus Disease (EVD) at the point of care have the potential to increase access and acceptability of EVD testing and the speed of patient isolation and secure burials for suspect cases. A pilot program for EVD RDTs in high risk areas of Guinea was introduced in October 2015. This paper presents concordance data between EVD RDTs and PCR testing in the field as well as an assessment of the acceptability, feasibility, and quality assurance of the RDT program. Read More

    Internet-based biosurveillance methods for vector-borne diseases: Are they novel public health tools or just novelties?
    PLoS Negl Trop Dis 2017 Nov 30;11(11):e0005871. Epub 2017 Nov 30.
    Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America.
    Internet-based surveillance methods for vector-borne diseases (VBDs) using "big data" sources such as Google, Twitter, and internet newswire scraping have recently been developed, yet reviews on such "digital disease detection" methods have focused on respiratory pathogens, particularly in high-income regions. Here, we present a narrative review of the literature that has examined the performance of internet-based biosurveillance for diseases caused by vector-borne viruses, parasites, and other pathogens, including Zika, dengue, other arthropod-borne viruses, malaria, leishmaniasis, and Lyme disease across a range of settings, including low- and middle-income countries. The fundamental features, advantages, and drawbacks of each internet big data source are presented for those with varying familiarity of "digital epidemiology. Read More

    RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody production.
    PLoS Pathog 2017 Nov 29;13(11):e1006738. Epub 2017 Nov 29.
    Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
    Follicular T helper cells (TFH) are fundamental in orchestrating effective antibody-mediated responses critical for immunity against viral infections and effective vaccines. However, it is unclear how virus infection leads to TFH induction. We here show that dengue virus (DENV) infection of human dendritic cells (DCs) drives TFH formation via crosstalk of RIG-I-like receptor (RLR) RIG-I and MDA5 with type I Interferon (IFN) signaling. Read More

    Single shot of 17D vaccine may not confer life-long protection against yellow fever.
    Mem Inst Oswaldo Cruz 2018 Feb 27;113(2):135-137. Epub 2017 Nov 27.
    Instituto Evandro Chagas, Ananindeua, PA, Brasil.
    The yellow fever (YF) vaccine has been used since the 1930s to prevent YF, which is a severe infectious disease caused by the yellow fever virus (YFV), and mainly transmitted by Culicidae mosquitoes from the genera Aedes and Haemagogus . Until 2013, the World Health Organization (WHO) recommended the administration of a vaccine dose every ten years. A new recommendation of a single vaccine dose to confer life-long protection against YFV infection has since been established. Read More

    Strengthened Ebola surveillance in France during a major outbreak in West Africa: March 2014-January 2016.
    Epidemiol Infect 2017 12 23;145(16):3455-3467. Epub 2017 Nov 23.
    French National Public Health Agency,Saint-Maurice,France.
    Introduction An unprecedented outbreak of Ebola virus diseases (EVD) occurred in West Africa from March 2014 to January 2016. The French Institute for Public Health implemented strengthened surveillance to early identify any imported case and avoid secondary cases.

    Methods: Febrile travellers returning from an affected country had to report to the national emergency healthcare hotline. Read More

    Feeding-induced changes in allatostatin-A and short neuropeptide F in the antennal lobes affect odor-mediated host seeking in the yellow fever mosquito, Aedes aegypti.
    PLoS One 2017 22;12(11):e0188243. Epub 2017 Nov 22.
    Unit of Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden.
    Aedes aegypti is a model species in which the endogenous regulation of odor-mediated host seeking behavior has received some attention. Sugar feeding and host seeking in female A. aegypti are transiently inhibited following a blood meal. Read More

    Detection and quantification of dengue virus using a novel biosensor system based on dengue NS3 protease activity.
    PLoS One 2017 21;12(11):e0188170. Epub 2017 Nov 21.
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.
    Background: The traditional methods, plaque assays and immuno-focus assays, used to titrate infectious dengue virus (DENV) particles are time consuming and labor intensive. Here, we developed a DENV protease activity detection system (DENPADS) to visualize DENV infection in cells based on dengue protease activity.

    Methodology/principal Findings: Dengue NS3 protease cleaves NS4B-NS5. Read More

    Cardiac tamponade in a patient with severe dengue fever.
    Rev Soc Bras Med Trop 2017 Sep-Oct;50(5):701-705
    Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil.
    A 26-year-old postpartum female presented with symptoms characteristic of dengue fever on the 16th day of puerperium. On the third day of the illness, the patient presented a clinical picture consistent with shock. Tests determined primary infection with dengue virus serotype 2. Read More

    Alert: Severe cases and deaths associated with Chikungunya in Brazil.
    Rev Soc Bras Med Trop 2017 Sep-Oct;50(5):585-589
    Departamento de Medicina Clínica, Faculdade de Medicina, Universidade Federal de Pernambuco, Recife, PE, Brasil.
    Since the detection of the Chikungunya virus in America in 2013, two million cases of the disease have been notified worldwide. Severe cases and deaths related to Chikungunya have been reported in India and Reunion Island, estimated at 1 death per 1,000 inhabitants. Joint involvement in the acute and chronic phase is the main clinical manifestation associated with Chikungunya. Read More

    Evaluation of the associations between endothelial dysfunction, inflammation and coagulation in Crimean-Congo hemorrhagic fever patients.
    Arch Virol 2018 Mar 20;163(3):609-616. Epub 2017 Nov 20.
    Department of Biochemistry, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
    Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne zoonotic viral disease. The aim of this study was to evaluate the association between inflammation, coagulation and endothelial dysfunction in CCHF. The study population consisted of 40 patients and 50 healthy controls. Read More

    Development and preliminary evaluation of a multiplexed amplification and next generation sequencing method for viral hemorrhagic fever diagnostics.
    PLoS Negl Trop Dis 2017 Nov 20;11(11):e0006075. Epub 2017 Nov 20.
    Highly Pathogenic Viruses, ZBS 1, Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany.
    Background: We describe the development and evaluation of a novel method for targeted amplification and Next Generation Sequencing (NGS)-based identification of viral hemorrhagic fever (VHF) agents and assess the feasibility of this approach in diagnostics.

    Methodology: An ultrahigh-multiplex panel was designed with primers to amplify all known variants of VHF-associated viruses and relevant controls. The performance of the panel was evaluated via serially quantified nucleic acids from Yellow fever virus, Rift Valley fever virus, Crimean-Congo hemorrhagic fever (CCHF) virus, Ebola virus, Junin virus and Chikungunya virus in a semiconductor-based sequencing platform. Read More

    Shared Genomic Variants: Identification of Transmission Routes Using Pathogen Deep-Sequence Data.
    Am J Epidemiol 2017 Nov;186(10):1209-1216
    Sequencing pathogen samples during a communicable disease outbreak is becoming an increasingly common procedure in epidemiologic investigations. Identifying who infected whom sheds considerable light on transmission patterns, high-risk settings and subpopulations, and the effectiveness of infection control. Genomic data shed new light on transmission dynamics and can be used to identify clusters of individuals likely to be linked by direct transmission. Read More

    The introduction of dengue follows transportation infrastructure changes in the state of Acre, Brazil: A network-based analysis.
    PLoS Negl Trop Dis 2017 Nov 17;11(11):e0006070. Epub 2017 Nov 17.
    Fiocruz, Programa de Computação Científica (PROCC), Rio de Janeiro, RJ, Brazil.
    Human mobility, presence and passive transportation of Aedes aegypti mosquito, and environmental characteristics are a group of factors which contribute to the success of dengue spread and establishment. To understand this process, we assess data from dengue national and municipal basins regarding population and demographics, transportation network, human mobility, and Ae. aegypti monitoring for the Brazilian state of Acre since the first recorded dengue case in the year 2000 to the year 2015. Read More

    Resource Use and Costs of Dengue: Analysis of Data from Phase III Efficacy Studies of a Tetravalent Dengue Vaccine.
    Am J Trop Med Hyg 2017 Dec 12;97(6):1898-1903. Epub 2017 Oct 12.
    Dengue Vaccination Impact and Economics, Sanofi Pasteur, Lyon, France.
    A tetravalent dengue vaccine (CYD-TDV) has recently been approved in 12 countries in southeast Asia and Latin America for individuals aged 9-45 years or 9-60 years (age indication approvals vary by country) living in endemic areas. Data on utilization of medical and nonmedical resources as well as time lost from school and work were collected during the active phase of two phase III efficacy studies performed in 10 countries in the Asia-Pacific region and Latin America (NCT01373281; NCT01374516). We compared dengue-related resource utilization and costs among vaccinated and nonvaccinated participants. Read More

    The challenges of detecting and responding to a Lassa fever outbreak in an Ebola-affected setting.
    Int J Infect Dis 2018 Jan 11;66:65-73. Epub 2017 Nov 11.
    Ministry of Health Liberia, Congo Town, Tubman Boulevard, Monrovia, Montserrado, Liberia.
    Objectives: Lassa fever (LF), a priority emerging pathogen likely to cause major epidemics, is endemic in much of West Africa and is difficult to distinguish from other viral hemorrhagic fevers, including Ebola virus disease (EVD). Definitive diagnosis requires laboratory confirmation, which is not widely available in affected settings. The public health action to contain a LF outbreak and the challenges encountered in an EVD-affected setting are reported herein. Read More

    Optical coherence tomography angiography features of acute macular neuroretinopathy in dengue fever.
    Indian J Ophthalmol 2017 Nov;65(11):1235-1238
    Advanced Eye Center, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
    A 39-year-old female with dengue fever presented with decreased vision in both eyes. Visual acuity was 20/200 and 20/80 in the right eye (OD) and left eye (OS), respectively. Fundus showed granular, grayish-white lesions in the parafoveal region in OD. Read More

    Assessing current temporal and space-time anomalies of disease incidence.
    PLoS One 2017 13;12(11):e0188065. Epub 2017 Nov 13.
    Department of Biostatistics, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America.
    Approaches used to early and accurately characterize epidemiologic patterns of disease incidence in a temporal and spatial series are becoming increasingly important. Cluster tests are generally designed for retrospective detection of epidemiologic anomalies in a temporal or space-time series. Timely identification of anomalies of disease or poisoning incidence during ongoing surveillance or an outbreak requires the use of sensitive statistical methods that recognize an incidence pattern at the time of occurrence. Read More

    Impact of the Ebola outbreak on Trypanosoma brucei gambiense infection medical activities in coastal Guinea, 2014-2015: A retrospective analysis from the Guinean national Human African Trypanosomiasis control program.
    PLoS Negl Trop Dis 2017 Nov 13;11(11):e0006060. Epub 2017 Nov 13.
    Univ. Bordeaux, Inserm, Infectious Diseases in Resource Limited Countries, U1219, ISPED, Bordeaux, France.
    Background: The 2014-2015 Ebola outbreak massively hit Guinea. The coastal districts of Boffa, Dubreka and Forecariah, three major foci of Human African Trypanosomiasis (HAT), were particularly affected. We aimed to assess the impact of this epidemic on sleeping sickness screening and caring activities. Read More

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