27,886 results match your criteria Vibrio Vulnificus Infection


Evaluation of hetero-multivalent lectin binding using a turbidity-based emulsion agglutination assay.

Colloids Surf B Biointerfaces 2018 Nov 28;175:84-90. Epub 2018 Nov 28.

Dept. of Chemical Engineering, Texas A&M University, College Station, TX, 77843, USA. Electronic address:

Lectin hetero-multivalency, binding to two or more different types of ligands, has been demonstrated to play a role in case of both LecA (a Pseudomonas aeruginosa adhesin) and Cholera Toxin subunit B (a Vibrio cholerae toxin). In order to screen the ligand candidates that involve in hetero-multivalent binding from large molecular libraries, we present a turbidity-based emulsion agglutination (TEA) assay that can be conducted in a high-throughput format using the standard laboratory instruments and reagents. The benefit of this assay is that it relies on the use of emulsions that can be formed using ultrasonication, minimizing the bottleneck of substrate surface functionalization. Read More

View Article
November 2018
1 Read

Virulence properties of Vibrio vulnificus isolated from diseased zoea of freshness shrimp Macrobrachium rosenbergii.

Microb Pathog 2018 Dec 3. Epub 2018 Dec 3.

College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. Electronic address:

Macrobrachium rosenbergii is one of the most economically important freshwater shimp, with fast growth and high nutrient content in the agricultural development of China. However, it had been suffering diseases infection, causing mass death and great economic losses. In the present study, a bacteria strain was isolated from the diseased zoea of M. Read More

View Article
December 2018

Marine probiotics: increasing coral resistance to bleaching through microbiome manipulation.

ISME J 2018 Dec 5. Epub 2018 Dec 5.

Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.

Although the early coral reef-bleaching warning system (NOAA/USA) is established, there is no feasible treatment that can minimize temperature bleaching and/or disease impacts on corals in the field. Here, we present the first attempts to extrapolate the widespread and well-established use of bacterial consortia to protect or improve health in other organisms (e.g. Read More

View Article
December 2018

Effects of soluble copper and copper oxide nanoparticle exposure on the immune system of mussels, Mytilus galloprovincialis.

Environ Toxicol 2018 Dec 2. Epub 2018 Dec 2.

Bodega Marine Laboratory, University of California Davis, Davis, California.

Copper and copper oxide nanomaterials (nCuO) can enter the marine environment negatively impacting mussels, an environmental and commercially relevant organism. We analyzed the effects on the immune system of adult mussels exposed to soluble copper (CuSO , 20-50 μg/L) or nCuO (100-450 μg/L). CuSO caused significant copper accumulation in gills and cell-free hemolymph, while nCuO caused cell damage to gills and significant copper accumulation in hemocytes, the most abundant cells in the hemolymph. Read More

View Article
December 2018

Sulfur availability for Vibrio fischeri growth during symbiosis establishment depends on biogeography within the squid light organ.

Mol Microbiol 2018 Dec 1. Epub 2018 Dec 1.

Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, 16802.

The fitness of host-associated microbes depends on their ability to access nutrients in vivo. Identifying these mechanisms is significant for understanding how microbes have evolved to fill specific ecological niches within a host. Vibrio fischeri is a bioluminescent bacterium that colonizes and proliferates within the light organ of the Hawaiian bobtail squid, which provides an opportunity to study how bacteria grow in vivo. Read More

View Article
December 2018

Improvement and evaluation of loop-mediated isothermal amplification combined with a chromatographic flow dipstick assay and utilization in detection of Vibrio cholerae.

Anal Bioanal Chem 2018 Dec 1. Epub 2018 Dec 1.

School of Medicine, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin, 300071, China.

Loop-mediated isothermal amplification (LAMP) is a specific, sensitive, and easy-to-perform nucleic acid analytical technique with wide application for diagnosis of disease. Recently, LAMP combined with use of a lateral chromatographic flow dipstick (LFD) has been widely used in nucleic acid detection. However, the LFD mechanism has not been systematically analyzed, and the optimal combination of labeled primers has not been adequately evaluated. Read More

View Article
December 2018

Survival, Virulent Characteristics, and Transcriptomic Analyses of the Pathogenic Under Starvation Stress.

Front Cell Infect Microbiol 2018 16;8:389. Epub 2018 Nov 16.

College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

Many bacteria have developed strategies for metamorphosis into sophisticated survival forms to survive extended periods of environmental stress. As a global causative agent of vibriosis in marine fish farming, () can efficiently grow and proliferate under environmental stress, but the specific mechanism is not clear. In the present study, survival, virulent characteristics, and transcriptomic analysis of the BH1 were performed under starvation stress. Read More

View Article
November 2018

Intergenerational effects of macroalgae on a reef coral: major declines in larval survival but subtle changes in microbiomes.

Mar Ecol Prog Ser 2018 23;589:97-114. Epub 2018 Feb 23.

School of Biological Sciences and Aquatic Chemical Ecology Center Georgia Institute of Technology, Atlanta, GA 30332-0230.

Tropical reefs are shifting from coral to macroalgal dominance, with macroalgae suppressing coral recovery, potentially via effects on coral microbiomes. Understanding how macroalgae affect corals and their microbiomes requires comparing algae- versus coral-dominated reefs without confounding aspects of time and geography. We compared survival, settlement, and post-settlement survival of larvae, as well as the microbiomes of larvae and adults, of the Pacific coral between an Marine Protected Area (MPA) dominated by corals versus an adjacent fished area dominated by macroalgae. Read More

View Article
February 2018

Biotechnological applications of quorum sensing inhibition as novel therapeutic strategies for multidrug resistant pathogens.

Microb Pathog 2018 Nov 29. Epub 2018 Nov 29.

Department of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Taibah University, Al Madinah Al Munawwarah, 30078, Saudi Arabia; Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, 35516, Egypt. Electronic address:

High incidence of antibiotic resistance among bacterial clinical isolates necessitates the discovery of new targets for inhibition of microbial pathogenicity without stimulation of microbial resistance. This could be achieved by targeting virulence determinants which cause host damage and disease. Many pathogenic bacteria elaborate signaling molecules for cellular communication. Read More

View Article
November 2018

Computational characterization and molecular dynamics simulation of the thermostable direct hemolysin-related hemolysin (TRH) amplified from Vibrio parahaemolyticus.

Microb Pathog 2018 Nov 29. Epub 2018 Nov 29.

Biotechnology Laboratory, ICAR-Central, Inland Fisheries Research Institute, Barrackpore, Kolkata, 700120, West Bengal, India.

Vibrio parahaemolyticus is a major seafood-borne pathogen that causes life-threatening gastroenteric diseases in humans through the consumption of contaminated seafoods. V. parahaemolyticus produces different kinds of toxins, including thermostable direct hemolysin (TDH), TDH-related hemolysin (TRH), and some effector proteins belonging to the Type 3 Secretion System, out of which TDH and TRH are considered to be the major factors for virulence. Read More

View Article
November 2018

Occurrence and virulence of Vibrio parahaemolyticus isolated from seawater and bivalve shellfish of the Gyeongnam coast, Korea, in 2004-2016.

Mar Pollut Bull 2018 Dec 23;137:382-387. Epub 2018 Oct 23.

Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea. Electronic address:

We determined the abundance and virulence of Vibrio parahaemolyticus in seawater and bivalves from the Gyeongnam coast in Korea, a major area for the seafood industry, during 2004-2016. V. parahaemolyticus is one of the most common pathogen causing seafood-borne illnesses in Korea, and increases during the summer. Read More

View Article
December 2018

Transcriptomic Approaches for Studying Quorum Sensing in Vibrio cholerae.

Methods Enzymol 2018 24;612:303-342. Epub 2018 Oct 24.

Faculty of Biology I, Department of Microbiology, Ludwig-Maximilians-University of Munich, Munich, Germany; Munich Center for Integrated Protein Science (CIPSM), Munich, Germany. Electronic address:

Transcriptome analysis using RNA-sequencing (RNA-seq) has now become the standard approach to determine the transcriptional output of an organism. Various modifications to this technology have been developed over the years, usually aiming to improve the annotation of transcript borders, or to identify novel classes of RNAs, such as small regulatory RNAs (sRNAs) and antisense transcripts. RNA-seq has also led to the identification of dozens of new sRNAs in the major human pathogen, Vibrio cholerae. Read More

View Article
October 2018
1 Read

Analysis of enzyme activity, antibacterial activity, antiparasitic activity and physico-chemical stability of skin mucus derived from Amphiprion clarkii.

Fish Shellfish Immunol 2018 Nov 28. Epub 2018 Nov 28.

State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, Fujian Province, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361102, Fujian Province, China; State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, Fujian Province, China. Electronic address:

Recently, mucosal surfaces, especially fish skin and its secreted mucus, have attracted significant interest from immunologists. Amphiprion clarkii, a member of the family Pomacentridae, lives symbiosis with sea anemones and has a good resistance to common seawater bacterial diseases and parasites owing to the protection from its abundant skin mucus. In the present work, the activity of immune-related enzymes (lysozyme, protease, antiprotease, cathepsin B, alkaline phosphatase and peroxidase), the antibacterial activity against two Gram-positive bacteria and five Gram-negative bacteria, the antiparasitic activity against the pathogen of marine white spot disease (Cryptocaryon irritans theronts) and the physico-chemical stability (to pH and heat) of the skin mucus of A. Read More

View Article
November 2018

Comparative study of stress and immune-related transcript outcomes triggered by Vibrio anguillarum bacterin and air exposure stress in liver and spleen of gilthead seabream (Sparus aurata), zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss).

Fish Shellfish Immunol 2018 Nov 28. Epub 2018 Nov 28.

The stress and immune-related effects of short-term (1, 6 and 24 h) air exposure stress (1 min), bath vaccination with Vibrio anguillarum bacterin, and both stressors combined were evaluated in liver and spleen of Sparus aurata, Danio rerio and Onchorhynchus mykiss. Expression profiles of immune (interleukin 1 beta: il1β; tumor necrosis factor alpha: tnfα; interleukin 10: il10; tumor growth factor beta: tgfβ1; immunoglobulin M: igm; lysozyme: lys; complement protein c3: c3) and stress-related genes (glucocorticoid receptor: gr; heat shock protein 70: hsp70; and enolase) were analysed by RT-qPCR. Cortisol level was assessed by radioimmunoassay. Read More

View Article
November 2018

VEGF-like protein from Apostichopus japonicus promotes cell proliferation and migration.

Dev Comp Immunol 2018 Dec 2. Epub 2018 Dec 2.

School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China.

Vascular endothelial growth factor (VEGF) is a key conservative regulator of inflammation response by promoting cell proliferation, migration, and vascular permeability. It also induces the release of inflammatory factors in vertebrates. We previously characterized NLR family pyrin domain containing 3 and HMGB3 homology in Apostichopus japonicus, providing the occurrence of inflammation in this species. Read More

View Article
December 2018
1 Read

Performance of Stool Testing Recommendations for Acute Gastroenteritis when Used to Identify Children with Nine Potential Bacterial Enteropathogens.

Clin Infect Dis 2018 Dec 4. Epub 2018 Dec 4.

Sections of Pediatric Emergency Medicine and Gastroenterology, Department of Pediatrics, Alberta Children's Hospital Research Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.

Background: The ability to identify bacterial pathogens that necessitate specific clinical management or public health action in children with acute gastroenteritis is crucial to patient care and public health. However, existing stool testing guidelines offer inconsistent recommendations, and their performance characteristics are unknown. We evaluated six leading gastroenteritis guidelines (e. Read More

View Article
December 2018
3 Reads

Matrix Assisted Regulation of Antimicrobial Properties: Mechanistic Elucidation with Ciprofloxacin-Based Polymeric Hydrogel Against Vibrio sp.

Bioconjug Chem 2018 Dec 5. Epub 2018 Dec 5.

Design of new drug material through modification of some well known antibiotics to combat pathogenic bacteria must include a complete understanding of matrix regulation, since the human body consists of majorly three types of matrices; solid, semisolid and liquid which has a tendency to regulate antibacterial efficacy along with bactericidal mechanism of several antimicrobial agents. Herein, matrix dependent action of ciprofloxacin based polymeric hydrogel scaffold was explored against a new species of Vibrio, namely Vibrio chemaguriensis Iso1 (V. chemaguriensis), which is resistant to most of the common antibiotics and possess genes that can be linked to pathogenicity. Read More

View Article
December 2018
2 Reads

Intramuscular administration of a DNA vaccine encoding OmpK antigen induces humoral and cellular immune response in flounder (Paralichthys olivaceus) and improves protection against Vibrio anguillarum.

Fish Shellfish Immunol 2018 Dec 1. Epub 2018 Dec 1.

Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No. 1 Wenhai Road, Aoshanwei Town, Qingdao, 266071, China.

Outer membrane protein K (OmpK) is an immunogenic protein that could act as subunit vaccine candidate for Vibrio anguillarum. In this study, a DNA vaccine encoding the OmpK gene of V. anguillarum was constructed and confirmed to express OmpK in vitro and in vivo. Read More

View Article
December 2018
1 Read

Cholera

Rev Prat 2017 12;67(10):1117-1121

Assistance publique-Hôpitaux de Marseille, hôpital de La Timone et Hôpital européen Marseille, Marseille, France.

Cholera. Cholera is caused by Vibrio cholerae, a Gram-negative bacteria. Some strains can synthesize an enterotoxin - the cholera toxin - which is responsible for the disease. Read More

View Article
December 2017
3 Reads

Potential application of SMART II for Vibrio cholerae O1 and O139 detection in ship's ballast water.

Mar Pollut Bull 2018 Nov 8;136:79-83. Epub 2018 Sep 8.

Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA; Maryland Institute of Applied Environmental Health, University of Maryland, College Park, MD, USA. Electronic address:

Ballast water is used to safely stabilize and operate shipping vessels worldwide, in a multitude of aquatic settings, including inland, coastal and open oceans. However, ballast water may pose ecological, public health, and/or economic problems as it may serve as vehicles of transmission of microorganisms. Current ballast water regulations include limits of Escherichia coli, Enterococcus spp. Read More

View Article
November 2018

Antimicrobial resistance and pulsed-field gel electrophoresis typing of Vibrio parahaemolyticus isolated from shrimp mariculture environment along the east coast of China.

Mar Pollut Bull 2018 Nov 14;136:164-170. Epub 2018 Sep 14.

East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Shanghai 200090, China. Electronic address:

Environmental antimicrobial resistance (AMR) has drawn increasing attention due to its great risk to human health. The aim of this study was to investigate AMR and genotyping of Vibrio parahaemolyticus isolates (n = 114) recovered from shrimp mariculture environment in China. The isolates exhibited a high rate of resistance to streptomycin (78. Read More

View Article
November 2018

Comparative study on degradation of propranolol and formation of oxidation products by UV/HO and UV/persulfate (PDS).

Water Res 2018 Oct 24;149:543-552. Epub 2018 Oct 24.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.

The frequent detection of propranolol, a widely used β-blocker, in wastewater effluents and surface waters has raised serious concern, due to its adverse effects on organisms. UV/hydrogen peroxide (UV/HO) and UV/persulfate (UV/PDS) processes are efficient in eliminating propranolol in various waters, but the formation of oxidation products in these processes, as well as the assessment of their toxicity, has not been systematically addressed. In this study, we identified and compared transformation products of propranolol produced by hydroxyl radical (OH) and sulfate radical (SO). Read More

View Article
October 2018

Evaluation of combination effects of Astragalus polysaccharides and florfenicol against acute hepatopancreatic necrosis disease-causing strain of Vibrio parahaemolyticus in Litopenaeus vannamei.

Fish Shellfish Immunol 2018 Nov 28;86:374-383. Epub 2018 Nov 28.

Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.

The effects of oral administration of Astragalus polysaccharides (APS) and florfenicol (FFC), singly or in combination, on the survival performance, disease resistance, and immunity of Litopenaeus vannamei were investigated. After challenge with an AHPND-causing strain of Vibrio parahaemolyticus (VP), shrimp were immediately fed a drug-free diet, diets containing only APS (200 mg·kg) or FFC (15 mg·kg), or diets containing low-dose (7.5 mg·kg FFC + 100 mg·kg APS), medium-dose (15 mg·kg FFC + 200 mg·kg APS), and high-dose (30 mg·kg FFC+400 mg·kg APS) drug combinations for 5 days. Read More

View Article
November 2018

Enhanced sensitivity to cholera toxin in female ADP-ribosylarginine hydrolase (ARH1)-deficient mice.

PLoS One 2018 30;13(11):e0207693. Epub 2018 Nov 30.

Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America.

Cholera toxin, an 84-kDa multimeric protein and a major virulence factor of Vibrio cholerae, uses the ADP-ribosyltransferase activity of its A subunit to intoxicate host cells. ADP-ribosylation is a posttranslational modification of proteins, in which the ADP-ribose moiety of NAD+ is transferred to an acceptor. In mammalian cells, ADP-ribosylation of acceptors appears to be reversible. Read More

View Article
November 2018
1 Read

Molecular characterization and expression analysis of galectins in Japanese pufferfish (Takifugu rubripes) in response to Vibrio harveyi infection.

Fish Shellfish Immunol 2018 Nov 27;86:347-354. Epub 2018 Nov 27.

Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, 116023, China. Electronic address:

Galectins are a family of proteins with conserved carbohydrate recognition domains (CRDs) that bind to specific glycans, including the glycans on the surface of pathogens, and therefore play a role in cytokine secretion, cell activation, migration, adhesion and apoptosis. Currently, galectins have been extensively studied in mammalian species but rarely studied in teleost fish species. In this study, a total of 12 galectin genes were characterized to understand the molecular mechanisms of galectin function in Japanese pufferfish (Takifugu rubripes). Read More

View Article
November 2018

C-type lectin 17A and macrophage-expressed receptor genes are magnified by fungal β-glucan after Vibrio parahaemolyticus infection in Totoaba macdonaldi cells.

Immunobiology 2018 Oct 18. Epub 2018 Oct 18.

Immunology & Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, La Paz, B.C.S, 23096, Mexico. Electronic address:

C-type lectins are a principal carbohydrate recognition mechanism as glucans on cell surfaces. This study identified and investigated molecular characterization and immune roles of a novel c-type lectin 17A from Totoaba macdonaldi (TmCLEC17A), which were described in head-kidney leukocytes after immunostimulation with fungal β-glucan 197A and Vibrio parahaemolyticus infection. This nucleotide sequence from totoaba was acquired using NGS and bioinformatics tools. Read More

View Article
October 2018

Identification of novel sRNAs involved in oxidative stress response in the fish pathogen Vibrio alginolyticus by transcriptome analysis.

J Fish Dis 2018 Nov 29. Epub 2018 Nov 29.

Department of Biology, Shantou University, Shantou, China.

Vibrio alginolyticus as an important pathogen in aquaculture can encounter the oxidative stress produced by the immune system during infection. Previous studies showed that sRNAs have important functions in response to oxidative stress in bacteria; however, less of sRNAs related to oxidative stress response were identified in V. alginolyticus. Read More

View Article
November 2018

Comparative genomics for non-O1/O139 Vibrio cholerae isolates recovered from the Yangtze River Estuary versus V. cholerae representative isolates from serogroup O1.

Mol Genet Genomics 2018 Nov 28. Epub 2018 Nov 28.

Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China Ministry of Agriculture, College of Food Science and Technology, Shanghai Ocean University, Shanghai, People's Republic of China.

Vibrio cholerae, which is autochthonous to estuaries worldwide, can cause human cholera that is still pandemic in developing countries. A number of V. cholerae isolates of clinical and environmental origin worldwide have been subjected to genome sequencing to address their phylogenesis and bacterial pathogenesis, however, little genome information is available for V. Read More

View Article
November 2018

Gene Duplication Drives the Evolution of Redundant Regulatory Pathways Controlling Expression of the Major Toxic Secreted Metalloproteases in LGP32.

mSphere 2018 Nov 28;3(6). Epub 2018 Nov 28.

Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

CsrBs are bacterial highly conserved and multiple-copy noncoding small RNAs (sRNAs) that play major roles in cell physiology and virulence. In the genus, they are known to be regulated by the two-component system VarS/VarA. They modulate the well-characterized quorum sensing pathway controlling virulence and luminescence in and , respectively. Read More

View Article
November 2018
1 Read

Relation between Biofilm and Virulence in Vibrio tapetis: A Transcriptomic Study.

Pathogens 2018 Nov 26;7(4). Epub 2018 Nov 26.

Laboratoire de Biotechnologie et Chimie Marines (LBCM), EA 3884, LBCM, IUEM Université de Bretagne-Sud, 56100 Lorient, France.

Marine pathogenic bacteria are able to form biofilms on many surfaces, such as mollusc shells, and they can wait for the appropriate opportunity to induce their virulence. can develop such biofilms on the inner surface of shells of the clam, leading to the formation of a brown conchiolin deposit in the form of a ring, hence the name of the disease: Brown Ring Disease. The virulence of is presumed to be related to its capacity to form biofilms, but the link has never been clearly established at the physiological or genetic level. Read More

View Article
November 2018
6 Reads

Toxigenic V. cholerae, V. parahaemolyticus, and V. vulnificus in oysters from the Gulf of Mexico and sold in Mexico City.

Int J Environ Health Res 2018 Nov 27:1-11. Epub 2018 Nov 27.

d Centro de Investigación Científica y de Educación Superior de Ensenada , Ensenada , Mexico.

Oysters can accumulate potentially pathogenic bacteria, such as Vibrio cholerae, V. parahaemolyticus, and V. vulnificus. Read More

View Article
November 2018

Structural analysis and proteomics studies on the Myoviridae vibriophage M4.

Arch Virol 2018 Nov 27. Epub 2018 Nov 27.

Division of Electron Microscopy, ICMR-National Institute of Cholera and Enteric Diseases, P-33 C.I.T Road, Scheme-XM, Beleghata, Kolkata, 700010, India.

Bacteriophages play a crucial role in tracking the spread of bacterial epidemics. The frequent emergence of antibiotic-resistant bacterial strains throughout the world has motivated studies on bacteriophages that can potentially be used in phage therapy as an alternative to conventional antibiotic treatment. A recent outbreak of cholera in Haiti took many lives due to a rapid development of resistance to the available antibiotics. Read More

View Article
November 2018
1 Read

Variations in the immune and metabolic response of proactive and reactive Sparus aurata under stimulation with Vibrio anguillarum vaccine.

Sci Rep 2018 Nov 26;8(1):17352. Epub 2018 Nov 26.

CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208, Matosinhos, Portugal.

Environmental insults, such as exposure to pathogens, modulate the behavioural coping style of animals to stressors, and repeated exposure to stressful environments may lead to species-specific infection phenotypes. To analyse the influence of stress behavioural phenotypes on immune and metabolic performance, gilthead sea bream (Sparus aurata L.) were first screened for proactive and reactive coping styles. Read More

View Article
November 2018
2 Reads

Regulatory Hierarchies Controlling Virulence Gene Expression in and .

Front Microbiol 2018 9;9:2686. Epub 2018 Nov 9.

Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin, Ireland.

Gram-negative enteropathogenic bacteria use a variety of strategies to cause disease in the human host and gene regulation in some form is typically a part of the strategy. This article will compare the toxin-based infection strategy used by the non-invasive pathogen , the etiological agent in human cholera, with the invasive approach used by , the cause of bacillary dysentery. Despite the differences in the mechanisms by which the two pathogens cause disease, they use environmentally-responsive regulatory hierarchies to control the expression of genes that have some features, and even some components, in common. Read More

View Article
November 2018
3 Reads

Biological activities of three medicinal plants from district Mirpur, AJK, Pakistan.

Pak J Pharm Sci 2018 Nov;31(6):2341-2346

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

Present research work was aimed to investigate the biological activities i.e. antibacterial, antifungal, antioxidant, cytotoxic and antitumor activities of crude methanolic extract of Anagallis arvensis L. Read More

View Article
November 2018
3 Reads

Effect of temperature on the microflora community composition in the digestive tract of the veined rapa whelk (Rapana venosa) revealed by 16S rRNA gene sequencing.

Comp Biochem Physiol Part D Genomics Proteomics 2018 Oct 23;29:145-153. Epub 2018 Oct 23.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address:

The veined rapa whelk, Rapana venosa, a poikilotherm that is susceptible to temperature, is an important and valuable fishery resource in China but a major invader around the world. We studied the effects of abnormal temperature on the digestive tract microflora of R. venosa to investigate how temperature impacts its digestion and ingestion. Read More

View Article
October 2018

Profiles of bacterial assemblages from microplastics of tropical coastal environments.

Sci Total Environ 2018 Nov 17;655:313-320. Epub 2018 Nov 17.

St. John Island National Marine Laboratory, Tropical Marine Science Institute (TMSI), National University of Singapore, 18 Kent Ridge Road, 119227, Singapore.

Plastic waste is a global issue of an increasing concern in aquatic ecosystems. Microplastics form a large proportion of plastic pollution in marine environments. Although microplastics are prevalent, their distribution along the coasts of tropical regions is not well studied. Read More

View Article
November 2018
4 Reads

Degradation of the Nonsteroidal Anti-Inflammatory Drug Piroxicam by Iron Activated Persulfate: The Role of Water Matrix and Ultrasound Synergy.

Int J Environ Res Public Health 2018 Nov 21;15(11). Epub 2018 Nov 21.

Department of Environmental Engineering, University of Western Macedonia, GR-50100 Kozani, Greece.

This work examined the oxidation of Piroxicam (PIR), a representative nonsteroidal anti-inflammatory drug using iron activated persulfate. The effect of persulfate dosing was vital for the efficiency of the process. The addition of 20 mg/L sodium persulfate (SPS) eliminated 500 μg/L of PIR in less than 20 min at natural pH. Read More

View Article
November 2018

Effects of dietary Bacillus subtilis and Shewanella algae in expression profile of immune-related genes from hemolymph of Litopenaeus vannamei challenged with Vibrio parahaemolyticus.

Fish Shellfish Immunol 2018 Nov 20;86:253-259. Epub 2018 Nov 20.

Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil. Electronic address:

B. subtilis and S. algae effects in growth, survival and innate immunity were assessed on L. Read More

View Article
November 2018
1 Read

A novel LRR-only protein mediates bacterial proliferation in hemolymph through regulating expression of antimicrobial peptides in mollusk Chlamys farreri.

Dev Comp Immunol 2018 Nov 20. Epub 2018 Nov 20.

CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, National Laboratory for Marine Science and Technology, Qingdao, 266237, China; CAS Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266400, China. Electronic address:

Leucine-rich repeat (LRR)-only proteins are involved in innate immune responses through mediating protein-ligand or protein-protein interactions, yet the exact roles of most LRR-only proteins in invertebrates are not well documented. In the present study, a novel LRR-only protein (designated CfLRRop-7) was identified in Zhikong scallop Chlamys farreri. The full-length cDNA sequence of CfLRRop-7 was 1463 bp and contained an open reading frame of 1086 bp, which encoded a protein of 361 amino acids. Read More

View Article
November 2018
4 Reads

The 9-Fluoren Vinyl Ether Derivative SAM461 Inhibits Bacterial Luciferase Activity and Protects From Luminescent Vibriosis.

Front Cell Infect Microbiol 2018 8;8:368. Epub 2018 Nov 8.

Instituto Universitario de Bio-Orgánica "Antonio González", Centro de Investigaciones Biomédicas de Canarias, Universidad de La Laguna, Tenerife, Spain.

is a major pathogen in aquaculture. It is a causative agent of the so-called "luminescent vibriosis," a life-threatening condition caused by bioluminescent spp. that often involves mass mortality of farmed shrimps. Read More

View Article
November 2018
2 Reads

Enhanced Biofilm Formation and Membrane Vesicle Release by Expressing a Commonly Occurring Plasmid Gene, .

Front Microbiol 2018 7;9:2605. Epub 2018 Nov 7.

Department of Molecular Biology, The Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, Umeå University, Umeå, Sweden.

is one of the most prevalent microorganisms forming biofilms on indwelling medical devices, as well as a representative model to study the biology and ecology of biofilms. Here, we report that a small plasmid gene, , enhances biofilm formation of . The gene is widely conserved among naturally occurring colicinogenic plasmids such as ColE1 plasmid, and is also present in some plasmid derivatives used as cloning vectors. Read More

View Article
November 2018
2 Reads

Chemometric Assessment and Best-Fit Function Modelling of the Toxic Potential of Selected Food Packaging Extracts.

Molecules 2018 Nov 20;23(11). Epub 2018 Nov 20.

Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza, 80-233 Gdańsk, Poland.

Food packaging materials constitute an ever more threatening environmental pollutant. This study examined options to specifically assess the ecotoxicity of packaged wastes, such as cans, subjected to various experimental treatments (in terms of extraction media, time of exposure, and temperature) that imitate several basic conditions of the process of food production. The extracts were studied for their ecotoxicity with bioluminescent bacteria. Read More

View Article
November 2018

Mechanistic basis of vitamin B12 and cobinamide salvaging by the Vibrio species.

Biochim Biophys Acta Proteins Proteom 2018 Nov 18;1867(2):140-151. Epub 2018 Nov 18.

Department of Biotechnology, St. Xavier's College, 30 Park Street, Kolkata 700016, India. Electronic address:

Biosynthesis of vitamin B12, which occurs through salvaging pathway or de novo synthesis, is essential for the survival and growth of bacteria. While the mechanism is known for many bacteria, it is elusive yet for diarrhea causing pathogenic bacteria Vibrio cholerae or the other Vibrio species. Sequence analysis using genome databases delineated that majority of the Vibrio species including V. Read More

View Article
November 2018

The antioxidant power of horseradish, Armoracia rusticana, underlies antimicrobial and antiradical effects, exerted in vitro.

Nat Prod Res 2018 Nov 21:1-4. Epub 2018 Nov 21.

b Institute for Coastal Marine Environment (IAMC), National Council Research (CNR) , Messina , Italy.

Armoracia rusticana (AR) was tested for antimicrobial and antioxidants power. The compound demonstrated to inhibit fish pathogens such as Vibrio anguillarum, V. harvey, V. Read More

View Article
November 2018
2 Reads

Characterization and CRISPR-based genotyping of clinical -positive .

Gut Pathog 2018 13;10:48. Epub 2018 Nov 13.

1Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand.

Background: is a causative agent of gastroenteritis. Most of the clinical isolates carry either and/or genes which are considered as the major virulence genes of this pathogen. In this study, the clinical isolates of carrying gene (= 73) obtained from 1886 to 2012 from various countries were investigated for the urease production, haemolytic activity, and biofilm formation. Read More

View Article
November 2018
4 Reads

Immune and Antioxidant Enzyme Response of Longfin Yellowtail (Seriola rivoliana) Juveniles to Ultra-diluted Substances Derived from Phosphorus, Silica and Pathogenic Vibrio.

Homeopathy 2018 Nov 20. Epub 2018 Nov 20.

Centro de Investigaciones Biológicas del Noroeste S.C. (CIBNOR), La Paz, Baja California Sur, México.

Background:  This research aimed to observe the effect of homeopathic treatments prepared from and (H1) and commercial homeopathic medication and (H2) on the immune and antioxidant response in juveniles under usual culture conditions and challenged with .

Methods:  Quantitative polymerase chain reaction analysis was used to study changes in the expression of key genes related to immune response, cytokines (interleukin-1β [IL-1β]), adapter protein for cytokine release (MyD88) and piscidin and spectrophotometric techniques to analyze the activity of antioxidant superoxide dismutase (SOD) and catalase (CAT) enzymes in juveniles at 30 (weaning stage [WS]) and 60 (early juveniles [EJ]) days post-hatching.

Results:  The H1 treatment led to over-expression of the IL-1β and MyD88 genes in fish at WS and EJ with respect to control, contrary to the H2 treatment that led to under-expression of the IL-1β, MyD88 and piscidin genes at the EJ stage. Read More

View Article
November 2018
11 Reads

Dissemination of Genetic Acquisition/Loss Provides a Variety of Quorum Sensing Regulatory Properties in Pseudoalteromonas.

Int J Mol Sci 2018 Nov 18;19(11). Epub 2018 Nov 18.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.

bstract Quorum sensing (QS) enables single-celled bacteria to communicate with chemical signals in order to synchronize group-level bacterial behavior. are marine bacteria found in versatile environments, of which QS regulation for their habitat adaptation is extremely fragmentary. To distinguish genes required for QS regulation in s, comparative genomics was deployed to define the pan-genomics for twelve isolates and previously-sequenced genomes, of which acyl-homoserine lactone (AHL)-based QS traits were characterized. Read More

View Article
November 2018
2 Reads
2.862 Impact Factor

New Invasive Nemertean Species () in England with High Levels of Tetrodotoxin and a Microbiome Linked to Toxin Metabolism.

Mar Drugs 2018 Nov 16;16(11). Epub 2018 Nov 16.

Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, Weymouth, Dorset DT4 8UB, UK.

The marine nemertean originates from the Pacific Ocean but in recent years has been discovered in northern Europe. The species has been associated with high levels of the marine neurotoxin Tetrodotoxin, traditionally associated with Pufferfish Poisoning. This study reports the first discovery of two organisms of in the UK, showing the geographical extent of this species is wider than originally described. Read More

View Article
November 2018
2 Reads

Fabrication of MgO nanostructures and its efficient photocatalytic, antibacterial and anticancer performance.

J Photochem Photobiol B 2018 Nov 12;190:8-20. Epub 2018 Nov 12.

Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappali 620 024, Tamilnadu, India.

Magnesium oxide (MgO) nanostructures were prepared using microwave-assisted (M 1) and hydrothermal (M 2) methods and characterized by XRD, SEM and FT-IR. It exhibits cubic structure with an average crystallite size of 20 nm (M 1) and 14 nm (M 2) and the lattice strain (WH plot) is 0.0017 (M 1), 0. Read More

View Article
November 2018
3 Reads