45,280 results match your criteria Vesicular Palmoplantar Eczema


Epidemiological features and spatial clusters of hand, foot, and mouth disease in Qinghai Province, China, 2009-2015.

BMC Infect Dis 2018 Dec 5;18(1):624. Epub 2018 Dec 5.

Institute for Infectious Disease Control and Prevention, Qinghai Provincial Center for Disease Control and Prevention, Xining, Qinghai, China.

Background: Hand, Foot, and Mouth Disease (HFMD) is most frequently caused by Enterovirus71 (EV-A71) or Coxsackie virus A16 (CV-A16), infants and young children are at greatest risk. Describing the epidemiology of HFMD can help develop and better target interventions, including the use of pediatric EV-A71 vaccination.

Methods: We obtained data from the national surveillance system for HFMD cases with onset dates from 2009 to 2015. Read More

View Article
December 2018
4 Reads

Optimization of Production of Vesicular Stomatitis Virus (VSV) in suspension serum-free culture medium at high cell density.

J Biotechnol 2018 Nov 30. Epub 2018 Nov 30.

Department of Bioprocess Engineering, National Research Council Canada, Building Montreal, Montréal, Canada; Department of Bioengineering McGill University, Montréal, Canada.

During the last decade, oncolytic viruses such as vesicular stomatitis virus (VSV) have gained tremendous popularity as efficient vaccines for infectious diseases as well as for the treatment of cancer. Our laboratory has developed two stable cell lines, 293SF-3F6 (derived from HEK293 A cells) and SF-BMAdR cells (a variant of A549 that expresses the E1 region of human adenovirus). These two cell lines were adapted to grow efficiently in suspension culture and in serum-free medium. Read More

View Article
November 2018

Different functional states of fusion protein gB revealed on human cytomegalovirus by cryo electron tomography with Volta phase plate.

PLoS Pathog 2018 Dec 3;14(12):e1007452. Epub 2018 Dec 3.

Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, United States of America.

Human cytomegalovirus (HCMV) enters host by glycoprotein B (gB)-mediated membrane fusion upon receptor-binding to gH/gL-related complexes, causing devastating diseases such as birth defects. Although an X-ray crystal structure of the recombinant gB ectodomain at postfusion conformation is available, the structures of prefusion gB and its complex with gH/gL on the viral envelope remain elusive. Here, we demonstrate the utility of cryo electron tomography (cryoET) with energy filtering and the cutting-edge technologies of Volta phase plate (VPP) and direct electron-counting detection to capture metastable prefusion viral fusion proteins and report the structures of glycoproteins in the native environment of HCMV virions. Read More

View Article
December 2018
1 Read

Oncogenic dependence of glioma cells on kish/TMEM167A regulation of vesicular trafficking.

Glia 2018 Dec 2. Epub 2018 Dec 2.

Instituto Cajal, CSIC, Madrid, Spain.

Genetic lesions in glioblastoma (GB) include constitutive activation of PI3K and EGFR pathways to drive cellular proliferation and tumor malignancy. An RNAi genetic screen, performed in Drosophila melanogaster to discover new modulators of GB development, identified a member of the secretory pathway: kish/TMEM167A. Downregulation of kish/TMEM167A impaired fly and human glioma formation and growth, with no effect on normal glia. Read More

View Article
December 2018

Unilateral periorbital swelling with vesicular golden crust forehead rash.

J Paediatr Child Health 2018 Dec;54(12):1397

Paediatric Department, Hospital Tuanku Ja'afar Seremban, Malaysia.

View Article
December 2018

Unilateral periorbital swelling with vesicular golden crust forehead rash.

J Paediatr Child Health 2018 Dec;54(12):1394

Paediatric Department, Hospital Tuanku Ja'afar Seremban, Malaysia.

View Article
December 2018

Transcytosis Assay for Transport of Glycosphingolipids across MDCK-II Cells.

Bio Protoc 2018 Oct;8(20)

Division of Gastroenterology, Boston Children's Hospital, Boston, Massachusetts 02115, USA.

Absorption and secretion of peptide and protein cargoes across single-cell thick mucosal and endothelial barriers occurs by active endocytic and vesicular trafficking that connects one side of the epithelial or endothelial cell (the lumen) with the other (the serosa or blood). Assays that assess this pathway must robustly control for non-specific and passive solute flux through weak or damaged intercellular junctions that seal the epithelial or endothelial cells together. Here we describe an cell culture Transwell assay for transcytosis of therapeutic peptides linked covalently to various species of the glycosphingolipid GM1. Read More

View Article
October 2018

Potent Antitumor T-Cell Memory Is Generated by Curative Viral Oncolytic Immunotherapy But Not Curative Chemotherapy.

Anticancer Res 2018 Dec;38(12):6621-6629

Department of Pediatrics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, Pittsburgh, PA, U.S.A.

Background: Late developing breast cancer metastases are common and lethal despite treatment with adjuvant chemotherapy at the time of primary tumor excision. Stimulation of an antitumor immune response is an alternative strategy for preventing this devastating development.

Materials And Methods: A mouse model of the human epidermal growth factor receptor 2 (HER2/neu)-positive mammary cancer was used to compare the antitumor memory T-cell response following tumor cure by viral oncolytic immunotherapy, chemotherapy, surgical excision, or surgical excision plus virus infection. Read More

View Article
December 2018
1 Read

Effective angiogenesis requires regulation of phosphoinositide signaling.

Adv Biol Regul 2018 Nov 26. Epub 2018 Nov 26.

Cancer Program, Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Victoria, 3800, Australia. Electronic address:

Phosphoinositide signaling regulates numerous downstream effectors that mediate cellular processes which influence cell cycle progression, migration, proliferation, growth, survival, metabolism and vesicular trafficking. A prominent role for phosphoinositide 3-kinase, which generates phosphatidylinositol 3,4,5-trisphosphate, a phospholipid that activates a plethora of effectors including AKT and FOXO during embryonic and postnatal angiogenesis, has been described. In addition, phosphatidylinositol 3-phosphate signaling is required for endosomal trafficking, which contributes to vascular remodeling. Read More

View Article
November 2018

Etidronate attenuates tactile allodynia by spinal ATP release inhibition in mice with partial sciatic nerve ligation.

Naunyn Schmiedebergs Arch Pharmacol 2018 Dec 4. Epub 2018 Dec 4.

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, 981-8558, Japan.

Etidronate is widely used as a therapeutic agent for osteoporosis. We have recently shown that intrathecal administration of etidronate into mice produces an analgesic effect against the capsaicin-induced nociceptive behavior. However, the effect of etidronate on neuropathic pain at the spinal level remains unknown. Read More

View Article
December 2018
2 Reads

Coupling factors involved in preserving bone balance.

Cell Mol Life Sci 2018 Dec 4. Epub 2018 Dec 4.

Division of Endocrinology and Metabolism, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul, 05505, South Korea.

Coupling during bone remodeling refers to the spatial and temporal coordination of bone resorption with bone formation. Studies have assessed the subtle interactions between osteoclasts and osteoblasts to preserve bone balance. Traditionally, coupling research related to osteoclast function has focused on bone resorption activity causing the release of growth factors embedded in the bone matrix. Read More

View Article
December 2018

Rhabdoviruses, Antiviral Defense, and SUMO Pathway.

Viruses 2018 12 3;10(12). Epub 2018 Dec 3.

INSERM UMR-S 1124, Université Paris Descartes, 75006 Paris, France.

Small Ubiquitin-like MOdifier (SUMO) conjugation to proteins has essential roles in several processes including localization, stability, and function of several players implicated in intrinsic and innate immunity. In human, five paralogs of SUMO are known of which three are ubiquitously expressed (SUMO1, 2, and 3). Infection by rhabdoviruses triggers cellular responses through the activation of pattern recognition receptors, which leads to the production and secretion of interferon. Read More

View Article
December 2018

The large protein 'L' of Peste-des-petits-ruminants virus exhibits RNA triphosphatase activity, the first enzyme in mRNA capping pathway.

Virus Genes 2018 Dec 3. Epub 2018 Dec 3.

Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.

Peste-des-petits-ruminants is a highly contagious and fatal disease of goats and sheep caused by non-segmented, negative strand RNA virus belonging to the Morbillivirus genus-Peste-des-petits-ruminants virus (PPRV) which is evolutionarily closely related to Rinderpest virus (RPV). The large protein 'L' of the members of this genus is a multifunctional catalytic protein, which transcribes and replicates the viral genomic RNA as well as possesses mRNA capping, methylation and polyadenylation activities; however, the detailed mechanism of mRNA capping by PPRV L protein has not been studied. We have found earlier that the L protein of RPV has RNA triphosphatase (RTPase), guanylyltransferase (GTase) and methyltransferase activities, and unlike vesicular stomatitis virus (VSV), follows the conventional pathway of mRNA capping. Read More

View Article
December 2018
3 Reads

A SUMOylation dependent switch of Rab7 governs intracellular life and pathogenesis of Typhimurium.

J Cell Sci 2018 Dec 3. Epub 2018 Dec 3.

Regional Centre for Biotechnology, 3 milestone Gurgaon Faridabad Expressway, Faridabad, 121001, India

Typhimurium (ST) is an intracellular pathogen that causes gastroenteritis in humans. Aided by a battery of effector proteins, ST resides intracellularly in a specialized vesicle, called -containing vacuole (SCV) that utilizes the host endocytic vesicular-transport pathway (VTP). Here we probed the possible role of SUMOylation, a post-translation modification pathway, in SCV biology. Read More

View Article
December 2018

GABA release selectively regulates synapse development at distinct inputs on direction-selective retinal ganglion cells.

Proc Natl Acad Sci U S A 2018 Dec 3. Epub 2018 Dec 3.

Department of Biological Structure, University of Washington, Seattle, WA 98195;

Synaptic inhibition controls a neuron's output via functionally distinct inputs at two subcellular compartments, the cell body and the dendrites. It is unclear whether the assembly of these distinct inhibitory inputs can be regulated independently by neurotransmission. In the mammalian retina, γ-aminobutyric acid (GABA) release from starburst amacrine cells (SACs) onto the dendrites of on-off direction-selective ganglion cells (ooDSGCs) is essential for directionally selective responses. Read More

View Article
December 2018

Management of placental site trophoblastic tumor: Two case reports.

Medicine (Baltimore) 2018 Nov;97(48):e13439

Interdisciplinary Department of Medicine, Gynecology and Obstetrics Clinic, University of Bari.

Rationale: Placental site trophoblastic tumor (PSTT) is a very rare malignant tumor, belonging to a family of pregnancy-related illnesses, called gestational trophoblastic diseases (GTD). Less than 300 cases of PSTT have been reported in literature, with an incidence of ≈ 1/50,000-100,000 pregnancies representing only 0.23% to 3. Read More

View Article
November 2018
2 Reads

Prolactin prevents the kainic acid-induced neuronal loss in the rat hippocampus by inducing prolactin receptor and putatively increasing the VGLUT1 overexpression.

Neurosci Lett 2018 Nov 27;694:116-123. Epub 2018 Nov 27.

Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Sto. Tomás, 11340, Ciudad de México, Mexico. Electronic address:

Neuroprotective effects of short prolactin (PRL) pre-treatment against kainic acid (KA)-induced damage include neuron loss avoidance in all hippocampal regions and attenuation of seizures. Recent evidence points PRL receptor (PRL-R) as mediator of such neuroprotective effects and seizures as regulators of neuronal marker transcript expression in the hippocampus. Here, we investigated if a daily PRL dose of 100 μg or vehicle for 14 days in ovariectomized rats (OVX) prevents neuron loss induced by KA administered on the third day of PRL treatment in a systemic single dose of 7. Read More

View Article
November 2018

The Isoform Selective Roles of PI3Ks in Dendritic Cell Biology and Function.

Front Immunol 2018 15;9:2574. Epub 2018 Nov 15.

Institut Necker Enfants Malades, Institut National de la Santé et de la Recherche Médicale, Unité 1151, Paris, France.

Phosphoinositide-3 kinases (PI3Ks) generate 3-phosphorylated phosphoinositide lipids that are implicated in many biological processes in homeostatic states and pathologies such as cancer, inflammation and autoimmunity. Eight isoforms of PI3K exist in mammals and among them the class I PI3K, p110γ, and PI3Kδ, and class III Vps34 being the most expressed and well characterized in immune cells. Following engagement of pathogen recognition receptors (PRRs), PI3Ks coordinate vital cellular processes of signaling and vesicular trafficking in innate phagocytes such as macrophages and professional antigen presenting dendritic cells (DCs). Read More

View Article
November 2018
4 Reads

Highly Stable Artificial Cells from Galactopyranose-Derived Single-Chain Amphiphiles.

J Am Chem Soc 2018 Nov 29. Epub 2018 Nov 29.

Single-chain amphiphiles (SCAs) capable of spontaneously self-assembling into large vesicular structures are attractive components of synthetic cells due to the simplicity of bilayer formation and increased membrane permeability. However, SCAs commonly used for vesicle formation suffer from a range of problems, including restricted working pH ranges, instability to free divalent cations, and the inhibition of biocatalysts. Construction of more robust membranes from SCAs, particularly in the presence of biological catalysts, would have significant benefits. Read More

View Article
November 2018

Cell cycle arrest in G2/M phase enhances replication of interferon-sensitive cytoplasmic RNA viruses via inhibition of antiviral gene expression.

J Virol 2018 Nov 28. Epub 2018 Nov 28.

Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte North Carolina, USA

Vesicular stomatitis virus (VSV, a rhabdovirus) and its variant VSV-ΔM51 are widely used model systems to study mechanisms of virus-host interactions. Here, we investigated how cell cycle affects replication of these viruses using an array of cell lines with different levels of impairment of antiviral signaling, and a panel of chemical compounds arresting cell cycle at different phases. We observed that all compounds inducing cell cycle arrest in G2/M phase strongly enhanced replication of VSV-ΔM51 in cells with functional antiviral signaling. Read More

View Article
November 2018

Penoscrotal lymphangioma circumscriptum following circumcision.

Turk J Urol 2018 Sep 1;44(5):437-440. Epub 2018 Sep 1.

Department of General Surgery, Post Graduate Institute of Medical Education And Research (PGIMER), Chandigarh, India.

We present a 15 year old boy with multiple vesicular lesions involving the scrotum and penile skin which developed following circumcision. Surgical excision of the penoscrotal skin with local flap cover was done. Histopathology was consistent with lymphangioma circumscriptum. Read More

View Article
September 2018

Potent Anti-Proliferation on the Colon Cancer Cell Line (HT-29) of Liposomal Formulations Entrapped with Semi-Purified Job's Tears ( Linn.) Fractions.

J Nanosci Nanotechnol 2019 Apr;19(4):1996-2007

Manose Health and Beauty Research Center, 179 Moo 10, Klong Cholprathan Road, Chiang Mai 50200, Thailand.

Liposomes, entrapped with Job's Tears fractions, were prepared by the supercritical carbon dioxide fluid (scCO₂) technique with and without sonication. Physical characteristics, which were the particle size, zeta potential, vesicular morphology, microviscosity and bioactivities including anti-proliferative, apoptotic and antioxidative activities of the S1L-S5L liposomal systems, were investigated. The potent anti-proliferative activity with the IC value of 4. Read More

View Article

Carvacrol Attenuates Hippocampal Neuronal Death after Global Cerebral Ischemia via Inhibition of Transient Receptor Potential Melastatin 7.

Cells 2018 Nov 26;7(12). Epub 2018 Nov 26.

Department of Physiology, College of Medicine, Hallym University, Chuncheon 24252, Korea.

Over the last two decades, evidence supporting the concept of zinc-induced neuronal death has been introduced, and several intervention strategies have been investigated. Vesicular zinc is released into the synaptic cleft, where it then translocates to the cytoplasm, which leads to the production of reactive oxygen species and neurodegeneration. Carvacrol inhibits transient receptor potential melastatin 7 (TRPM7), which regulates the homeostasis of extracellular metal ions, such as calcium and zinc. Read More

View Article
November 2018

CUTANEOUS MANIFESTATIONS OF HEMATOLOGIC MALIGNANCIES.

Hematol Oncol 2018 Nov 28. Epub 2018 Nov 28.

Di.S.Sal. Section of Dermatology, University of Genoa, San Martino Polyclinic Hospita, Genoa, Italy.

In recent years, dermatologic manifestations in oncohematologic patients have become more common. The aim of our study was to determine the incidence and heterogeneity of skin manifestations in patients followed at our Hematology Department. This observational monocentrical study was conducted on 60 patients. Read More

View Article
November 2018
2 Reads

Glutamate, glutamate transporters, and circadian rhythm sleep disorders in neurodegenerative diseases.

ACS Chem Neurosci 2018 Nov 28. Epub 2018 Nov 28.

Glutamate, a primary excitatory neurotransmitter as well as an important intermediate of the cellular metabolism in the brain, has a widespread influence in the sleep-wake regulatory system. Glutamate transporters, including vesicular glutamate transporters and excitatory amino acid transporters, serve as the main force to control the extracellular concentration of glutamate in the brain. They are likely to be critical tools needed for the brain to modulate the sleep-wake cycle, and likely innervated by the circadian rhythm system in a day-night variant pattern either. Read More

View Article
November 2018
1 Read

A triply-responsive supramolecular vesicle fabricated by α-cyclodextrin based host-guest recognition and double dynamic covalent bonds.

Soft Matter 2018 Nov 28. Epub 2018 Nov 28.

Agricultural University of Hebei, College of Science, Baoding 071001, P. R. China.

The supramolecular construction of multi-stimuli assemblies is a challenging task for prospective use. In this work, a novel supramolecular amphiphile was fabricated by introducing molecules with dynamic covalent bonds into host-guest inclusion. The amphiphile formed a vesicle, which was characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), 1H NMR and UV-vis spectra. Read More

View Article
November 2018

Strong stimulation triggers full fusion exocytosis and very slow endocytosis of the small dense core granules in carotid glomus cells.

J Neurogenet 2018 Sep;32(3):267-278

a Department of Pharmacology and Neuroscience and Metal Health Institute , University of Alberta , Edmonton , Canada.

Chemosensory glomus cells of the carotid bodies release transmitters, including ATP and dopamine mainly via the exocytosis of small dense core granules (SDCGs, vesicular diameter of ∼100 nm). Using carbon-fiber amperometry, we showed previously that with a modest uniform elevation in cytosolic Ca concentration ([Ca] of ∼0.5 µM), SDCGs of rat glomus cells predominantly underwent a "kiss-and-run" mode of exocytosis. Read More

View Article
September 2018

Different Subgroups of Cholinergic Neurons in the Basal Forebrain Are Distinctly Innervated by the Olfactory Regions and Activated Differentially in Olfactory Memory Retrieval.

Front Neural Circuits 2018 13;12:99. Epub 2018 Nov 13.

University of Chinese Academy of Sciences (UCAS), Beijing, China.

The mammalian basal forebrain (BF), a heterogenous structure providing the primary cholinergic inputs to cortical and limbic structures, plays a crucial role in various physiological processes such as learning/memory and attention. Despite the involvement of the BF cholinergic neurons (BFCNs) in olfaction related memory has been reported, the underlying neural circuits remain poorly understood. Here, we combined viral trans-synaptic tracing systems and ChAT-cre transgenic mice to systematically reveal the relationship between the olfactory system and the different subsets of BFCNs. Read More

View Article
November 2018
3 Reads
3.570 Impact Factor

Sympathetic Hyperactivity and Age Affect Segregation and Expression of Neurotransmitters.

Front Cell Neurosci 2018 13;12:411. Epub 2018 Nov 13.

Departamento de Biología Celular & Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México, Mexico.

Sympathetic neurons of the rat superior cervical ganglion (SCG) can segregate their neurotransmitters and co-transmitters to separate varicosities of single axons. We have shown that transmitter segregation is a plastic phenomenon and that it is correlated with the strength of synaptic transmission. Here, we determined whether sympathetic dysfunction occurring in stress and hypertension was correlated with plastic changes of neurotransmitter segregation. Read More

View Article
November 2018

α-Synuclein Trafficking in Parkinson's Disease: Insights From Fly and Mouse Models.

ASN Neuro 2018 Jan-Dec;10:1759091418812587

1 School of Life Science and Technology, ShanghaiTech University, Shanghai, China.

Protein aggregation and accumulation are common pathological hallmarks in neurodegenerative diseases. To efficiently clear and eliminate such aggregation becomes an important cellular strategy for cell survival. Lewy bodies inclusion and aggregation of α-Synuclein (α-Syn) during the pathogenesis of Parkinson's disease (PD) serve as a good example and are potentially linked to other pathological PD features such as progressive dopaminergic neuron cell death, behavioral defects, and nonmotor symptoms like anosmia, cognitive impairment, and depression. Read More

View Article
November 2018

Cholinergic activity regulates the secretome of epicardial adipose tissue: Association with atrial fibrillation.

J Cell Physiol 2018 Nov 27. Epub 2018 Nov 27.

Cardiology Group, Health Research Institute of Santiago de Compostela, Santiago de Compostela, Spain.

Botulinum toxin injection on epicardial fat, which inhibits acetylcholine (ACh) release, reduced the presence of atrial fibrillation (AF) in patients after heart surgery. Thus, we wanted to study the profile of the released proteins of epicardial adipose tissue (EAT) under cholinergic activity (ACh treatment) and their value as AF predictors. Biopsies, explants, or primary cultures were obtained from the EAT of 85 patients that underwent open heart surgery. Read More

View Article
November 2018
1 Read

Glutamate receptors and white matter stroke.

Neurosci Lett 2018 Nov 23;694:86-92. Epub 2018 Nov 23.

Achucarro Basque Center for Neuroscience, CIBERNED and Department of Neuroscience, University of the Basque Country, Leioa, Spain. Electronic address:

White matter (WM) damage during ischemia occurs at multiple sites including myelin, oligodendrocytes, astrocytes and axons. A major driver of WM demise is excitoxicity as a consequence of excessive glutamate release by vesicular and non-vesicular mechanisms from axons and glial cells. This results in over-activation of ionotropic glutamate receptors (GluRs) profusely expressed by all cell compartments in WM. Read More

View Article
November 2018

Factors Associated With Cysticidal Treatment Response in Extraparenchymal Neurocysticercosis.

J Clin Pharmacol 2018 Nov 26. Epub 2018 Nov 26.

Neuroinflammation Unit, IIBM-UNAM/INNN/Facultad de Medicina- UNAM, Ciudad de México, México.

Extraparenchymal neurocysticercosis is the most severe form of cysticercosis, and response to treatment is suboptimal. We sought to determine how demographic and clinical characteristics and albendazole sulfoxide concentrations were related to cysticidal treatment response. We conducted a longitudinal study of 31 participants with extraparenchymal vesicular parasites who received the same treatment, albendazole 30 mg/kg/day for 10 days with dexamethasone 0. Read More

View Article
November 2018
4 Reads

Treatment of oral manifestations of toxic epidermal necrolysis with low-level laser therapy in a pediatric patient.

Pediatr Dermatol 2018 Nov 26. Epub 2018 Nov 26.

Department of Oral Surgery and Pathology, Faculty of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Drug-induced reactions are complications associated with high mortality and significant morbidity. Stevens-Johnson Syndrome (SJS) and toxic epidermal necrolysis (TEN) are examples of these conditions, which are characterized by skin and mucous lesions. Here, we report a case of a 9-year-old girl who presented with blisters associated with an extensive vesicular rash and multiple ulcerations on the lips and oral cavity. Read More

View Article
November 2018
3 Reads

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay.

J Vis Exp 2018 Nov 11(141). Epub 2018 Nov 11.

Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba; Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba; Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences;

The Rho GTPase family belongs to the Ras superfamily and includes approximately 20 members in humans. Rho GTPases are important in the regulation of diverse cellular functions, including cytoskeletal dynamics, cell motility, cell polarity, axonal guidance, vesicular trafficking, and cell cycle control. Changes in Rho GTPase signaling play an essential regulatory role in many pathological conditions, such as cancer, central nervous system diseases, and immune system-dependent diseases. Read More

View Article
November 2018
4 Reads

Limbic encephalitis: Experience of a moroccan center.

Brain Behav 2018 Nov 25:e01177. Epub 2018 Nov 25.

Department of Neurology, Hassan II University Teaching Hospital, Fez, Morocco.

Objectives: Histologically defined as an inflammation-degeneration of limbic structures, limbic encephalitis (LE) is a rare disease and often difficult to diagnose particularly in institutions with limited access to laboratory tests such as antineuronal antibodies or HSV-PCR, and functional imaging. We aimed to describe the demographic, clinical, paraclinical, and etiological features of LE, as well as its medium-term prognosis in Moroccan patients.

Materials And Methods: We collected retrospectively all patients diagnosed with LE in the Department of Neurology of the University Hospital Hassan II of Fez (Morocco) between September 2008 and December 2016. Read More

View Article
November 2018
5 Reads

Combination of virotherapy with VSV and tumor vaccination significantly enhances the efficacy of antitumor therapy.

Acta Virol 2018 ;62(4):394-400

Oncolytic virotherapy offers the potential to treat tumors both as a single agent and in combination with conventional therapies such as chemotherapy and immunological therapy. Here, we describe an effective treatment regimen which combines virotherapy with immunotherapy. IFN-α and co-stimulator IL-2 along with tumor cell lysate vaccination with intratumoral administration of oncolytic vesicular stomatitis virus (VSV) resulted in regression of established TC1 papilloma tumor model in C57BL/6 mice. Read More

View Article
January 2018
3 Reads

Does the Surgical Timing and Decompression Alone or Fusion Surgery in Lumbar Stenosis Influence Outcome in Cauda Equina Syndrome?

Asian Spine J 2018 Nov 27. Epub 2018 Nov 27.

Department of Spine Surgery, Stavya Spine Hospital and Research Institute, Ahmedabad, India.

Study Design: A retrospective comparative analysis of 64 patients with cauda equina syndrome (CES), who underwent either decompression alone (NF) or fusion (F) surgery.

Purpose: We compared the outcomes and timing effects.

Overview Of Literature: CES can cause loss of autonomic control of vesicular function and lower limb neurological deficits. Read More

View Article
November 2018
6 Reads

Diet-Induced Insulin Resistance Elevates Hippocampal Glutamate as well as VGLUT1 and GFAP Expression in AβPP/PS1 Mice.

J Neurochem 2018 Nov 25. Epub 2018 Nov 25.

Department of Neurology, Center for Alzheimer's Disease and Related Disorders, Neurosciences Institute.

The symptomologies of Alzheimer's disease (AD) develop over decades suggesting modifiable life-style factors may contribute to disease pathogenesis. In humans, hyperinsulinemia associated with type 2 diabetes mellitus increases the risk for developing AD and both diseases share similar age-related etiologies including amyloidogenesis. Since we have demonstrated that soluble Aβ elicits glutamate release, we wanted to understand how diet-induced insulin resistance alters hippocampal glutamate dynamics, which are important for memory formation and consolidation. Read More

View Article
November 2018
3 Reads

Decreased pallidal vesicular monoamine transporter type 2 availability in Parkinson's disease: The contribution of the nigropallidal pathway.

Neurobiol Dis 2018 Nov 22;124:176-182. Epub 2018 Nov 22.

The Edmond J. Safra Program in Parkinson's Disease & Movement Disorder Unit, Toronto Western Hospital, University Health Network, University of Toronto, Ontario, Canada; Krembil Research Institute, University Health Network, University of Toronto, Ontario, Canada; Research Imaging Centre, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, University of Toronto, Ontario, Canada. Electronic address:

To date, the contribution of the nigropallidal pathway degeneration to Parkinson's disease (PD) motor symptoms has received little attention and is generally poorly understood in spite of solid evidence that the globus pallidus (GP) receives a dense neuronal projection from the substantia nigra. To explore the dopaminergic (DA) changes of the GP in PD, we measured the availability of vesicular monoamine transporter 2 (VMAT2) using [C]DTBZ and positron emission tomography in 30 PD patients and 12 controls. PD patients were classified in two groups based on severity of disease. Read More

View Article
November 2018
3 Reads

Remodeling of cholinergic input to the hippocampus after noise exposure and tinnitus induction in Guinea pigs.

Hippocampus 2018 Nov 24. Epub 2018 Nov 24.

Kresge Hearing Research Institute, Department of Otolaryngology, University of Michigan, Ann Arbor, Michigan.

Here, we investigate remodeling of hippocampal cholinergic inputs after noise exposure and determine the relevance of these changes to tinnitus. To assess the effects of noise exposure on the hippocampus, guinea pigs were exposed to unilateral noise for 2 hr and 2 weeks later, immunohistochemistry was performed on hippocampal sections to examine vesicular acetylcholine transporter (VAChT) expression. To evaluate whether the changes in VAChT were relevant to tinnitus, another group of animals was exposed to the same noise band twice to induce tinnitus, which was assessed using gap-prepulse Inhibition of the acoustic startle (GPIAS) 12 weeks after the first noise exposure, followed by immunohistochemistry. Read More

View Article
November 2018
2 Reads

Sorbitol-penetration enhancer containing vesicles loaded with baicalin for the protection and regeneration of skin injured by oxidative stress and UV radiation.

Int J Pharm 2018 Nov 20;555:175-183. Epub 2018 Nov 20.

Dept. of Scienze della Vita e dell'Ambiente, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.

Aiming at improving the protective effects of baicalin on the skin, new highly-biocompatible penetration enhancer containing vesicles (PEVs) were developed by modifying the base formulation of transfersomes with sorbitol, thus obtaining sorbitol-PEVs. An extensive evaluation of the physico-chemical features of both transfersomes and sorbitol-PEVs was carried out. Transfersomes were mainly close-packed, multi-compartment vesicles, while sorbitol-PEVs appeared mostly as single, spherical, unilamellar vesicles. Read More

View Article
November 2018
1 Read

Antiapoptotic role of Agaricus blazei extract in rodent model of Parkinson's disease.

Front Biosci (Elite Ed) 2019 01 1;11:12-19. Epub 2019 Jan 1.

Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu 600073, India.

Rotenone is a pesticide that has been shown to induce the pathological symptoms of Parkinson's disease (PD) in both cellular and animal models. In this study, we investigated the protective effect of Agaricus blazei extract on rotenone-induced dopaminergic degeneration and apoptosis in mice model. A. Read More

View Article
January 2019
3 Reads

A study on preganglionic connections and possible viscerofugal projections from urinary bladder intramural ganglia to the caudal mesenteric ganglion in the pig.

J Anat 2018 Nov 23. Epub 2018 Nov 23.

Department of Human Physiology, School Of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.

The present study was designed to (1) ascertain the distribution and immunohistochemical characteristics of sympathetic preganglionic neurons supplying the caudal mesenteric ganglion (CaMG) and (2) verify the existence of viscerofugal projections from the urinary bladder trigone intramural ganglia (UBT-IG) to the CaMG in female pigs (n = 6). Combined retrograde tracing and immunofluorescence methods were used. Injections of the neuronal tracer Fast Blue (FB) into the right CaMG revealed no retrogradely labelled (FB-positive; FB ) nerve cells in the intramural ganglia; however, many FB neurons were found in the spinal cord sympathetic nuclei. Read More

View Article
November 2018
1 Read

Catestatin regulates vesicular quanta through modulation of cholinergic and peptidergic (PACAPergic) stimulation in PC12 cells.

Cell Tissue Res 2018 Nov 22. Epub 2018 Nov 22.

Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0732, USA.

We have previously shown that the chromogranin A (CgA)-derived peptide catestatin (CST: hCgA) inhibits nicotine-induced secretion of catecholamines from the adrenal medulla and chromaffin cells. In the present study, we seek to determine whether CST regulates dense core (DC) vesicle (DCV) quanta (catecholamine and chromogranin/secretogranin proteins) during acute (0.5-h treatment) or chronic (24-h treatment) cholinergic (nicotine) or peptidergic (PACAP, pituitary adenylyl cyclase activating polypeptide) stimulation of PC12 cells. Read More

View Article
November 2018
1 Read

Bile Acids and Their Derivatives as Potential Modifiers of Drug Release and Pharmacokinetic Profiles.

Front Pharmacol 2018 8;9:1283. Epub 2018 Nov 8.

Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia.

Bile acids have received considerable interest in the drug delivery research due to their peculiar physicochemical properties and biocompatibility. The main advantage of bile acids as drug absorption enhancers is their ability to act as both drug solubilizing and permeation-modifying agents. Therefore, bile acids may improve bioavailability of drugs whose absorption-limiting factors include either poor aqueous solubility or low membrane permeability. Read More

View Article
November 2018
2 Reads

USP14 promotes K63-linked RIG-I deubiquitination and suppresses antiviral immune responses.

Eur J Immunol 2018 Nov 22. Epub 2018 Nov 22.

Institute of Immunology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Retinoic acid-inducible gene I (RIG-I) is a critical RNA virus sensor that initiates antiviral immune response through K63-linked ubiquitination. In this study, we demonstrated USP14, a deubiquitinating enzyme, as a negative regulator in antiviral responses by directly deubiquitinating K63-linked RIG-I. USP14 knockdown significantly enhanced RIG-I-triggered type I IFN signaling and inhibited vesicular stomatitis virus (VSV) replication both in mouse peritoneal macrophages and THP1 cells. Read More

View Article
November 2018
6 Reads

Ganoderma tsugae induced ROS-independent apoptosis and cytoprotective autophagy in human chronic myeloid leukemia cells.

Food Chem Toxicol 2018 Nov 19;124:30-44. Epub 2018 Nov 19.

Institute of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, 40402, Taiwan. Electronic address:

The medicinal fungus Ganoderma, known in Chinese as Lingzhi or Reishi, traditionally has various medicinal uses and has been employed in cancer treatment in Asia for centuries. This study used ethanol-extracted Ganoderma tsugae (GT) and examined its antitumor activities on human chronic myeloid leukemia cells as well as its molecular mechanism of action. Treatment with GT (200-400 μg/mL) significantly reduced cell viability and caused G2/M arrest in K562 cells. Read More

View Article
November 2018
2 Reads
2.900 Impact Factor

Presynaptic regulation of dopamine release: Role of the DAT and VMAT2 transporters.

Neurochem Int 2018 Nov 19;122:94-105. Epub 2018 Nov 19.

Department of Psychology, Brock University, St. Catharines, ON, L2S 3A1, Canada. Electronic address:

The signaling dynamics of the neurotransmitter dopamine has been established to have an important role in a variety of behavioural processes including motor control, cognition, and emotional processing. Key regulators of transmitter release and the signaling dynamics of dopamine are the plasma membrane reuptake transporter (DAT) and the vesicular monoamine transporter (VMAT2). These proteins serve to remove dopamine molecules from the extracellular and cytosolic space, respectively and both determine the amount of transmitter released from synaptic vesicles. Read More

View Article
November 2018
2 Reads

Cationic vesicles for efficient shRNA transfection in the MCF-7 breast cancer cell line.

Int J Nanomedicine 2018 6;13:7107-7121. Epub 2018 Nov 6.

Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran,

Introduction: Novel and safe delivery solutions for RNAi therapeutics are essential to obtain the full potential of cancer gene therapy.

Methods: In this study, cationic vesicular nanocarrier was applied for delivering lnc urothelial carcinoma-associated 1 (lnc UCA1) shRNA expression vector to MCF-7 cells. The physicochemical characteristics, cytotoxicity, and transfection efficiency of cationic vesicles prepared from various molar ratios of amphiphilic surfactant Tween 80 (T), squalene (S), cationic charge lipid didodecyldimethylammonium bromide, and polyethylenimine were investigated. Read More

View Article
November 2018
1 Read